Oil control composition as well as preparation method and application thereof

Abstract

The invention provides an oil control composition as well as a preparation method and application thereof. The oil control composition is prepared from the following components in parts by weight: 20 to 60 parts of rhizoma anemarrhenae root extract, 2 to 20 parts of swertia bimaculata extract and 10 to 90 parts of solvent. Experiments show that the oil control composition can play a role in inhibiting expression of regulatory factors SREBP-1, SCD1 and FASN, meanwhile, expression of AMPK is promoted, and generation of grease is effectively inhibited by precisely regulating and controlling the targets; on the other hand, the oil control composition can also promote expression of ABCA1 and accelerate discharge of generated grease, so that the comprehensive oil control effect is achieved.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to an oil control composition, a preparation method and application thereof. Background Art

[0002] In daily life, excessive skin oil secretion is a common problem that troubles many people. Excessive oil not only makes the skin surface greasy and sticky, but also easily absorbs dust, dirt and bacteria, which in turn clogs the pores and causes a series of skin problems, such as acne, blackheads, seborrheic dermatitis, etc., which seriously affect the health of the skin.

[0003] Based on this, oil control effect has become a hot topic in efficacy research within the industry, and is also one of the hot issues in people's increasingly diverse skin care needs.

[0004] The oil-control effect of current cosmetics is mainly achieved by inhibiting the 5α-reductase pathway to inhibit oil secretion. However, 5α-reductase is only one of the many regulatory factors of human oil production. If it only acts on 5α-reductase, its oil-control effect is not ideal. Summary of the invention

[0005] In view of the deficiencies of the prior art, the object of the present invention is to provide an oil control composition, a preparation method and its application, aiming to solve the technical problem that the prior art only inhibits oil secretion by inhibiting the 5α-reductase pathway, but has a poor oil control effect.

[0006] In order to achieve the above objectives, in a first aspect, the present invention provides an oil control composition, comprising the following components in parts by weight: 20 to 60 parts of an extract from the roots of Anemarrhena asphodeloides, 2 to 20 parts of an extract from Swertia japonica, and 10 to 90 parts of a solvent.

[0007] Furthermore, the invention comprises the following components in parts by weight: 30 to 50 parts of anemarrhena root extract, 5 to 10 parts of swertia japonica extract and 20 to 80 parts of a solvent.

[0008] Furthermore, the solvent includes at least one of glycerol, butylene glycol, propylene glycol and deionized water.

[0009] In a second aspect, the present invention provides a method for preparing an oil control composition, which is used to prepare the oil control composition as described in the first aspect above, and the method for preparing the oil control composition comprises the following steps:

[0010] Mixing the extract of the root of Anemarrhena asphodeloides with a solvent to form a first mixture, and performing a first stirring treatment on the first mixture to obtain a first material to be used;

[0011] Mixing the Swertia japonica extract with a solvent to form a second mixture, and performing a second stirring treatment on the second mixture to obtain a second material to be used;

[0012] The first object to be used and the second object to be used are mixed into a third mixture, and the third mixture is subjected to a third stirring process to obtain an oil control composition.

[0013] In a third aspect, the present invention provides a use of the oil control composition as described in the first aspect in preparing cosmetics.

[0014] Furthermore, the weight proportion of the oil control composition in the cosmetic is 0.01% to 30%.

[0015] Furthermore, the cosmetic is shampoo, conditioner, shower gel or facial cleanser.

[0016] Compared with the prior art, the beneficial effect of the present invention is that: experiments show that the oil control composition can inhibit the expression of regulatory factors SREBP-1, SCD1, and FASN, and promote the expression of AMPK, and effectively inhibit the production of oil by precisely regulating these targets; on the other hand, the oil control composition can also promote the expression of ABCA1, accelerate the discharge of generated oil, and thus achieve a comprehensive oil control effect. That is, compared with the method of inhibiting oil secretion only by inhibiting the pathway of 5α reductase, the composition of the present application can produce a regulatory effect at multiple targets, achieving a better comprehensive oil control effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 is a flow chart of the method for preparing the oil control composition in Example 6 of the present invention;

[0018] The following specific implementation manner will further illustrate the present invention in conjunction with the above-mentioned drawings. DETAILED DESCRIPTION

[0019] In order to facilitate the understanding of the present invention, the present invention will be described more fully below with reference to the relevant drawings. Several embodiments of the present invention are given in the drawings. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present invention more thorough and comprehensive.

[0020] It should be noted that when an element is referred to as being "fixed to" another element, it may be directly on the other element or there may be a central element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only.

[0021] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0022] See also Figure 1 In the first aspect, Example 1 of the present invention provides an oil control composition, comprising an extract from the roots of Anemarrhena asphodeloides, an extract from Swertia japonica and a solvent. Specifically, it comprises the following components in parts by weight: 20 parts of the extract from the roots of Anemarrhena asphodeloides, 2 parts of the extract from Swertia japonica and 10 parts of a solvent.

[0023] The extract of the root of Anemarrhena asphodeloides can be prepared by the following method: crushing the root of Anemarrhena asphodeloides decoction pieces into root of Anemarrhena asphodeloides particles, mixing the root of Anemarrhena asphodeloides particles in deionized water at a solid-liquid ratio of 1:10, reflux extraction at 90°C, the number of reflux extractions is 2 times, the reflux extraction time is 1.5 hours, to obtain a first extract and a second extract, mixing the first extract and the second extract into a first mixed extract, filtering and centrifuging the first mixed extract to obtain a first treated liquid, and performing reduced pressure concentration and drying on the first treated liquid to obtain the root of Anemarrhena asphodeloides extract;

[0024] The Swertia japonica extract can be prepared by the following method: washing the whole Swertia japonica herb and cutting it into sections, and performing hot air drying and crushing on the sections to obtain Swertia japonica particles, mixing the Swertia japonica particles in a 50% ethanol solution at a solid-liquid ratio of 1:15, and performing ultrasonic assisted extraction at 70°C, wherein the number of ultrasonic assisted extractions is 3 times and the time of ultrasonic assisted extraction is 30 minutes to obtain a third extract, a fourth extract and a fifth extract, mixing the third extract, the fourth extract and the fifth extract into a second mixed extract, filtering the second mixed extract and performing rotary evaporation concentration treatment to obtain the Swertia japonica extract.

[0025] Preferably, the solvent includes at least one of glycerol, butylene glycol, propylene glycol and deionized water. In this embodiment, the solvent includes butylene glycol, propylene glycol and deionized water. By using the butylene glycol, the propylene glycol and the deionized water to form the solvent, the solvent dissolves the effective substance to promote the penetration of the oil control composition, making the efficacy of the oil control composition more excellent.

[0026] Example 2 of the present invention provides an oil control composition, which is different from the oil control composition described in Example 1 in that:

[0027] The oil control composition comprises the following components by weight: 60 parts of anemarrhena root extract, 20 parts of swertia japonica extract and 90 parts of solvent.

[0028] Example 3 of the present invention provides an oil control composition, which is different from the oil control composition described in Example 1 in that:

[0029] The oil control composition comprises the following components by weight: 30 parts of anemarrhena root extract, 5 parts of swertia japonica extract and 20 parts of solvent.

[0030] Example 4 of the present invention provides an oil control composition, which is different from the oil control composition described in Example 1 in that:

[0031] The oil control composition comprises the following components in parts by weight: 50 parts of anemarrhena root extract, 10 parts of swertia japonica extract and 80 parts of a solvent.

[0032] Example 5 of the present invention provides an oil control composition, which is different from the oil control composition described in Example 1 in that:

[0033] The oil control composition comprises the following components by weight: 35 parts of anemarrhena root extract, 8 parts of swertia japonica extract and 55 parts of solvent.

[0034] Comparative Example 1 of the present invention provides an oil control composition, which is different from the oil control composition described in Example 1 in that:

[0035] The oil control composition comprises the following components by weight: 5 parts of anemarrhena root extract, 1 part of swertia japonica extract and 8 parts of solvent.

[0036] Comparative Example 2 of the present invention provides an oil control composition, which is different from the oil control composition described in Example 1 in that:

[0037] The oil control composition includes the following components by weight: 2 parts of Swertia japonica extract and 10 parts of solvent. That is, in this comparative example, the oil control composition does not use the Anemarrhena asphodeloides root extract.

[0038] Comparative Example 3 of the present invention provides an oil control composition, which is different from the oil control composition described in Example 1 in that:

[0039] The oil control composition comprises the following components by weight: 20 parts of Anemarrhena root extract and 10 parts of solvent. That is, in this comparative example, the oil control composition does not use Swertia japonica extract.

[0040] Comparative Example 4 of the present invention provides an oil control composition, which is different from the oil control composition described in Example 1 in that:

[0041] The oil control composition is prepared in a traditional manner, and the oil control composition inhibits oil secretion only by inhibiting the pathway of 5α-reductase.

[0042] The preparation methods of the oil control compositions described in Examples 1 to 5 and Comparative Examples 1 to 4 of the present invention were used to prepare oil control compositions, and the oil control effects of the different oil control compositions were tested respectively, specifically:

[0043] The golden hamsters were randomly divided into blank group, positive drug group and test group (10 groups were administered with Examples 1 to 5 and Comparative Examples 1 to 5, respectively), totaling 12 groups, 6 mice in each group. The area of ​​sebaceous glands on the back of each group of mice was measured before the experiment and on the last day of the experiment. The blank group was not given any special intervention; the positive drug group was given rosiglitazone tablets 4 mg / (kg·d) (dissolved in 2 mL of distilled water) by gavage, once a day, 0.1 mL / mouse each time; the test group was coated with 1 mL of the sample of the corresponding concentration, twice a day, and applied topically to the sebaceous gland spots on the back of the golden hamster. After 4 weeks of continuous intervention, 10% chloral hydrate was injected intraperitoneally for anesthesia, and skin tissue of sebaceous gland spots of about 1 cm×1 cm on both sides of the back was cut, placed in 4% formaldehyde solution for fixation, and marked for inspection. HE staining was used to observe the pathological manifestations of the sebaceous gland spots of each group of golden hamsters.

[0044] Furthermore, immunohistochemistry was used to detect the comprehensive expression of oil regulatory factors in sebaceous gland spots, and an image analysis system (Aipathwell) was used for analysis and calculation. Specifically, the average optical density of each regulatory factor protein in the sebaceous gland spot tissue of each group of golden hamsters was measured. The test method is as follows:

[0045] (I) Preparation of paraffin sections

[0046] 1. Fixation and sampling: Immediately place the skin tissue at the sebaceous gland spot into the tissue fixative for more than 24 hours, and store and transport at room temperature. Take the tissue out of the tissue fixative, trim the tissue with a scalpel in a fume hood, and place the trimmed tissue and the corresponding label in a dehydration box.

[0047] 2. Dehydration and wax dipping: put the dehydration box into the dehydration machine and dehydrate with graded alcohol in sequence. 75% alcohol for 4 hours, 85% alcohol for 2 hours, 90% alcohol for 2 hours, 95% alcohol for 1 hour, anhydrous ethanol I for 30 minutes, anhydrous ethanol II for 30 minutes, alcohol benzene for 5-10 minutes, xylene I for 5-10 minutes, xylene II for 5-10 minutes, 65° melted paraffin I for 1 hour, 65° melted paraffin II for 1 hour, 65° melted paraffin III for 1 hour to complete dehydration and wax dipping.

[0048] 3. Paraffin embedding: embed the wax-soaked tissue in the embedding machine. First, put the melted wax into the embedding frame. Before the wax solidifies, take the tissue out of the dehydration box and put it into the embedding frame according to the requirements of the embedding surface and affix the corresponding label. Cool it in a -20℃ freezer. After the wax solidifies, take the wax block out of the embedding frame and trim the wax block.

[0049] 4. Paraffin section: Place the trimmed wax block on a paraffin slicer and slice it to a thickness of 4μm. Float the slices on the 40℃ warm water of the slice spreader to flatten the tissue, pick up the tissue with a glass slide, and bake the slices in a 60℃ oven. After the wax is baked dry, take it out and store it at room temperature for later use.

[0050] (II) Immunohistochemistry experimental steps

[0051] 1. Dewax the paraffin sections to water: put the sections into environmentally friendly dewaxing liquid I for 10 minutes - environmentally friendly dewaxing liquid II for 10 minutes - environmentally friendly dewaxing liquid III for 10 minutes - anhydrous ethanol I for 5 minutes - anhydrous ethanol II for 5 minutes - anhydrous ethanol III for 5 minutes - distilled water to wash.

[0052] 2. Antigen repair: During this process, the buffer should be prevented from evaporating excessively and the slides should not be dried. After cooling naturally, place the slides in PBS (pH 7.4) and shake on a decolorizing shaker for 3 times, 5 minutes each time. (The repair solution and repair conditions are determined according to the tissue)

[0053] 3. Block endogenous peroxidase: Place the sections in 3% hydrogen peroxide solution and incubate at room temperature in the dark for 25 minutes. Place the slides in PBS (pH 7.4) and wash them three times on a decolorizing shaker, each time for 5 minutes.

[0054] 4. Serum blocking: Add 3% BSA in the histochemical circle to evenly cover the tissue, and block at room temperature for 30 minutes (if the primary antibody is from goat, use rabbit serum to block, and if it is from other sources, use BSA to block).

[0055] 5. Add primary antibody: Gently shake off the blocking solution, add primary antibody prepared in a certain proportion with PBS on the slices, and incubate the slices flat in a humidified box at 4°C overnight.

[0056] 6. Add secondary antibody: Place the slide in PBS (pH 7.4) and shake on a decolorizing shaker for 3 times, 5 minutes each time. After the slices are slightly dried, add the secondary antibody (HRP labeled) of the same species as the primary antibody in the circle to cover the tissue and incubate at room temperature for 50 minutes.

[0057] 7. DAB color development: Place the slide in PBS (pH 7.4) and shake on a decolorizing shaker for 3 times, 5 minutes each time. After the slices are slightly dried, add freshly prepared DAB color development solution in the circle, and control the color development time under a microscope. The positive color is brown-yellow, and the slices are rinsed with tap water to stop the color development.

[0058] 8. Re-staining of cell nuclei: Re-stain with hematoxylin for about 3 minutes, wash with tap water, differentiate with hematoxylin differentiation solution for a few seconds, rinse with tap water, re-blue with hematoxylin bluing solution, and rinse with running water.

[0059] 9. Dehydration and sealing: Put the slices into 75% alcohol for 5 minutes - 85% alcohol for 5 minutes - anhydrous ethanol I for 5 minutes - anhydrous ethanol II for 5 minutes - n-butanol for 5 minutes - xylene I for 5 minutes to dehydrate and make them transparent. Take the slices out of xylene, dry them slightly, and seal them with sealing glue.

[0060] 10. Microscopic examination: Place under a white light microscope to interpret the results.

[0061] The results of the sebaceous gland spot area detection and the average optical density measurement results of each regulatory factor protein are shown in Table 1 below:

[0062] Table 1

[0063]

[0064] As can be seen from the above table, the oil control composition described in Examples 1 to 5 of the present application is compared with the oil control composition described in Comparative Examples 1 to 4. After the components in the oil control composition described in Examples 1 to 4 of the present application are mixed, they play a good role in oil control. The oil control composition can inhibit the expression of regulatory factors SREBP-1, SCD1, and FASN, and promote the expression of AMPK. Through the precise regulation of these targets, the production of oil is effectively inhibited; on the other hand, the oil control composition can also promote the expression of ABCA1, accelerate the discharge of generated oil, and thus achieve a comprehensive oil control effect. That is, compared with the method of inhibiting oil secretion only by inhibiting the pathway of 5α reductase, the composition of the present application can produce a regulatory effect at multiple targets, achieving a better comprehensive oil control effect. The lack of its components has a greater impact on the effect of oil control. And by controlling the weight of each component, it can effectively ensure a more significant oil control effect.

[0065] See also Figure 1 In a second aspect, Example 6 of the present invention provides a method for preparing an oil control composition, which is used to prepare the oil control composition described in Examples 1 to 5 above, and the method for preparing the oil control composition comprises the following steps:

[0066] S10: mixing the extract of the root of Anemarrhena asphodeloides with a solvent to form a first mixture, and performing a first stirring treatment on the first mixture to obtain a first material to be used;

[0067] S20: mixing the Swertia japonica extract with a solvent to form a second mixture, and performing a second stirring treatment on the second mixture to obtain a second material to be used;

[0068] S30: mixing the first object to be used and the second object to be used into a third mixture, and performing a third stirring process on the third mixture to obtain an oil control composition.

[0069] In a third aspect, Example 7 of the present invention provides a use of the oil control composition described in Examples 1 to 5 above in the preparation of cosmetics.

[0070] Preferably, the weight proportion of the oil control composition in the cosmetic is 0.01% to 30%, and the cosmetic is shampoo, conditioner, shower gel or facial cleanser.

[0071] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.

[0072] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. An oil control composition, characterized in that: The invention comprises the following components in parts by weight: 20 to 60 parts of anemarrhena root extract, 2 to 20 parts of swertia japonica extract and 10 to 90 parts of solvent.

2. The oil control composition according to claim 1, characterized in that The invention comprises the following components in parts by weight: 30 to 50 parts of anemarrhena root extract, 5 to 10 parts of swertia japonica extract and 20 to 80 parts of solvent.

3. The oil control composition according to claim 1 or 2, characterized in that: The solvent includes at least one of glycerol, butylene glycol, propylene glycol and deionized water.

4. A method for preparing an oil control composition, for preparing the oil control composition according to any one of claims 1 to 3, characterized in that: The preparation method of the oil control composition comprises the following steps: Mixing the extract of the root of Anemarrhena asphodeloides with a solvent to form a first mixture, and performing a first stirring treatment on the first mixture to obtain a first material to be used; Mixing the Swertia japonica extract with a solvent to form a second mixture, and performing a second stirring treatment on the second mixture to obtain a second material to be used; The first object to be used and the second object to be used are mixed into a third mixture, and the third mixture is subjected to a third stirring process to obtain an oil control composition.

5. Use of the oil control composition according to any one of claims 1 to 3 in the preparation of cosmetics.

6. The use according to claim 5, characterized in that: The weight proportion of the oil control composition in the cosmetic is 0.01% to 30%.

7. The use according to claim 5, characterized in that: The cosmetic is shampoo, conditioner, shower gel or facial cleanser.

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