Production area processing method for improving content of index components of salvia miltiorrhiza

Abstract

The invention provides a producing area processing method for improving the content of index components of salvia miltiorrhiza, and belongs to the technical field of traditional Chinese medicine processing. The invention provides a production place processing method of salvia miltiorrhiza. The production place processing method comprises the following steps: performing adversity stress treatment on salvia miltiorrhiza; and drying the salvia miltiorrhiza subjected to adversity stress treatment. When the processing method of the radix salviae miltiorrhizae in the producing area is used for processing the fresh radix salviae miltiorrhizae medicinal material, the content of four index components including salvianolic acid B, cryptotanshinone, tanshinone I and tanshinone IIA in the radix salviae miltiorrhizae is remarkably increased, and further, the processing method of the radix salviae miltiorrhizae in the producing area has the effects of promoting the increase of secondary metabolites of the radix salviae miltiorrhizae and improving the yield of the radix salviae miltiorrhizae. The content of index components of salvia miltiorrhiza is increased, and meanwhile, the obtained dried salvia miltiorrhiza medicinal material is good in appearance property and good in condition. In conclusion, the radix salviae miltiorrhizae producing area processing method provided by the invention is low in cost, simple and feasible to operate in an adversity stress stage and a drying stage after stress, low in manpower and material resource consumption, environment-friendly, wide in application prospect and good in economic value.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicine processing, and particularly relates to a production site processing method for improving the content of index components of salvia miltiorrhiza. Background Art

[0002] Danshen is the dried root and rhizome of Salvia miltiorrhiza Bge., a plant of the Labiatae family. It is included in the 2020 edition of the Chinese Pharmacopoeia. It has the effects of promoting blood circulation and removing blood stasis, relieving pain by promoting menstruation, clearing the heart and eliminating vexation, cooling blood and eliminating carbuncle. It is used for chest pain, heart pain, abdominal pain, accumulation of masses, pain caused by heat arthralgia, restlessness and insomnia, irregular menstruation, dysmenorrhea and amenorrhea, and swelling and pain caused by sores. The main components of Danshen are phenolic acids and quinone compounds, among which the contents of salvianolic acid B, cryptotanshinone, tanshinone I and tanshinone II A are the indicator components of the 2020 edition of the Chinese Pharmacopoeia. Danshen is known as the "essential medicine for gynecology". As a major medicinal material with a long history of use in my country, it was included in the United States Pharmacopoeia in 2014 and plays an important role in the treatment of diseases and economic benefits. However, the current processing technology of fresh Salvia miltiorrhiza on the market is inefficient and the processing methods are not uniform. How to further improve the quality of Salvia miltiorrhiza, standardize and unify the processing methods at the origin, and provide higher quality Salvia miltiorrhiza Chinese medicinal materials has attracted widespread attention.

[0003] Processing at the origin is an important link that affects the quality of Chinese herbal medicines. Studies have found that processing methods at different origins have a significant impact on the quality of Chinese herbal medicines, especially some special processing can significantly increase the content of active ingredients in Chinese herbal medicines. At present, there is no unified processing method for the origin of Salvia miltiorrhiza. In actual production, most of them are directly dried after harvesting, but the drying temperature varies. Summary of the invention

[0004] In view of the defects in the prior art, the object of the present invention is to provide a production site processing method for improving the index components of Salvia miltiorrhiza, which can increase the accumulation of the index components during the harvesting and processing of Salvia miltiorrhiza.

[0005] The objective of the present invention is achieved through the following technical solutions:

[0006] The present invention provides a method for processing salvia miltiorrhiza, comprising the following steps:

[0007] The salvia miltiorrhiza is subjected to stress treatment; the temperature of the stress treatment is 30 to 60° C.; the humidity of the stress treatment is 70% to 98%; the time of the stress treatment is 0.5 to 3 hours;

[0008] The Salvia miltiorrhiza treated with adverse stress was dried.

[0009] Preferably, the temperature of the stress treatment is 30-50°C; the humidity of the stress treatment is 80%-98%; and the time of the stress treatment is 1-2 hours.

[0010] Preferably, the temperature of the stress treatment is 30-40°C; the humidity of the stress treatment is 80%-90%; and the time of the stress treatment is 2 hours.

[0011] Preferably, the drying method includes any one or more of sun drying, shade drying and oven drying.

[0012] Preferably, the drying temperature is 40-100°C.

[0013] Preferably, the drying temperature is 50-70°C.

[0014] The present invention provides salvia miltiorrhiza prepared by the origin processing method described in the above technical scheme.

[0015] Preferably, the mass percentage of the index components of the salvia miltiorrhiza is 4.90% to 8.96%; the index components include salvianolic acid B and tanshinones; the tanshinones include cryptotanshinone, tanshinone I and tanshinone IIA.

[0016] The present invention provides an application of the origin processing method described in the above technical solution in improving the content of index components of Salvia miltiorrhiza.

[0017] Preferably, the indicative components of Salvia miltiorrhiza include any one or two or more of salvianolic acid B, cryptotanshinone, tanshinone I and tanshinone IIA.

[0018] Beneficial effects of the present invention

[0019] The present invention provides a method for processing salvia miltiorrhiza at the place of origin, comprising the following steps: subjecting salvia miltiorrhiza to stress treatment; the temperature of the stress treatment is 30-60°C; the humidity of the stress treatment is 70%-98%; the stress treatment time is 0.5-3h; and the salvia miltiorrhiza treated with stress is dried. The method for processing at the place of origin provided by the present invention creates stress in the adverse environment before drying after the salvia miltiorrhiza is harvested, and performs stress treatment, so that the fresh salvia miltiorrhiza can maintain life activities for a long time and promote the secondary metabolism level of salvia miltiorrhiza, thereby improving the quality of salvia miltiorrhiza and increasing the accumulation of index component content during the harvesting and processing of salvia miltiorrhiza. The fresh salvia miltiorrhiza medicinal material is processed by the origin processing method of salvia miltiorrhiza provided by the present invention, which significantly increases the content of four index components of salvia miltiorrhiza, namely, salvianolic acid B, cryptotanshinone, tanshinone I and tanshinone ⅡA. Compared with direct drying of fresh medicinal materials, shade drying and sun drying treatments, and sweating treatments, the total content of index components of salvia miltiorrhiza medicinal materials is significantly improved, far exceeding the requirements of the 2020 edition of the "Chinese Pharmacopoeia". Furthermore, the origin processing method of salvia miltiorrhiza adopted by the present invention promotes the increase of secondary metabolites of salvia miltiorrhiza and improves the content of index components of salvia miltiorrhiza, while the obtained dried salvia miltiorrhiza medicinal material has good appearance and appearance. In summary, the origin processing method of salvia miltiorrhiza provided by the present invention is low in cost, simple and feasible to operate in both the adverse stress stage and the drying stage after stress, less consumption of manpower and material resources, environmentally friendly, and has broad application prospects and good economic value. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0021] Figure 1 This is a bar chart of the total content of index components of fresh Salvia miltiorrhiza samples in experimental case 1 after drying at different temperatures, sun drying, shade drying, drying after sweating, and drying after adverse stress;

[0022] Figure 2 The bar graph is a graph of the total content of indicative components of the fresh Salvia miltiorrhiza sample in Example 1 after being dried at 60° C. after being treated with different adverse stresses;

[0023] Figure 3 The bar graph of the total content of indicative components of the fresh Salvia miltiorrhiza samples in Example 2 after being treated with different adverse stresses and dried at 60°C;

[0024] Figure 4 The bar graph of the total content of indicative components of the fresh Salvia miltiorrhiza samples in Example 3 after being treated with different adverse stresses and dried at 60°C;

[0025] Figure 5 This is the HPLC chromatogram for the detection of index components of Salvia miltiorrhiza.

[0026] Figure 6 These are the appearance pictures of Salvia miltiorrhiza treated in different ways. DETAILED DESCRIPTION

[0027] The present invention provides a method for processing salvia miltiorrhiza, comprising the following steps:

[0028] The salvia miltiorrhiza is subjected to stress treatment; the temperature of the stress treatment is 30 to 60° C.; the humidity of the stress treatment is 70% to 98%; the time of the stress treatment is 0.5 to 3 hours;

[0029] The Salvia miltiorrhiza treated with adverse stress was dried.

[0030] The present invention treats Salvia miltiorrhiza with stress. In the present invention, the Salvia miltiorrhiza is preferably fresh Salvia miltiorrhiza; the fresh Salvia miltiorrhiza is preferably a two-year-old fresh Salvia miltiorrhiza in Weifang, Shandong. The present invention preferably pre-treats Salvia miltiorrhiza before treating Salvia miltiorrhiza with stress. The method for pre-treating Salvia miltiorrhiza in the present invention is not particularly limited, and any conventional Salvia miltiorrhiza pre-treatment method in the art can be used. As an optional embodiment of the present invention, the method for pre-treating Salvia miltiorrhiza may include removing fibrous roots, washing with clean water and sorting. After obtaining the pre-treated Salvia miltiorrhiza, the present invention treats Salvia miltiorrhiza with stress. In the present invention, the temperature of the stress treatment is 30 to 60°C, may be 30 to 50°C, or may be 30 to 40°C. As an optional embodiment of the present invention, the temperature of the stress treatment may be 30°C, 40°C, 50°C or 60°C. In the present invention, the humidity of the stress treatment is 70% to 98%, may be 80% to 98%, or may be 80% to 90%. As an optional embodiment of the present invention, the humidity of the adversity stress treatment can be 70%, 80%, 90% or 98%. In the present invention, the time of the adversity stress treatment is 0.5 to 3 hours, can be 1 to 3 hours, can be 1 to 2 hours, or can be 2 to 3 hours. As an optional embodiment of the present invention, the time of the adversity stress treatment can be 0.5 hours, 1 hour, 2 hours or 3 hours. After the adversity stress treatment is completed, the present invention obtains adversity stress-treated Salvia miltiorrhiza. The present invention creates adversity stress and performs adversity stress treatment, so that fresh Salvia miltiorrhiza can not only maintain life activities for a long time, but also promote the secondary metabolism level of Salvia miltiorrhiza, increase the accumulation of index component content during the harvesting and processing of Salvia miltiorrhiza, thereby improving the quality of Salvia miltiorrhiza.

[0031] After obtaining the salvia miltiorrhiza treated with adverse stress, the present invention dries the salvia miltiorrhiza treated with adverse stress. The present invention does not particularly limit the drying method, and any conventional drying method in the art can be used. The drying method can be any one or more of sun drying, shade drying and oven drying. When the present invention is dried, the drying temperature can be 40 to 100°C, 50 to 70°C, or 60°C. As an optional embodiment of the present invention, the drying temperature can be 40°C, 50°C, 60°C, 70°C, 80°C, 90°C or 100°C. After drying, the present invention obtains salvia miltiorrhiza processed at the place of origin.

[0032] The application of the origin processing method of Danshen provided by the present invention to fresh Danshen medicinal materials significantly increases the content of four index components in Danshen, namely, salvianolic acid B, cryptotanshinone, tanshinone I and tanshinone IIA. Compared with direct drying of fresh medicinal materials, shade drying, sun drying and sweating treatment, the total content of index components of Danshen medicinal materials is significantly increased, far exceeding the requirements of the 2020 edition of the "Chinese Pharmacopoeia". Furthermore, the origin processing method of Danshen adopted by the present invention promotes the increase of secondary metabolites of Danshen and improves the content of index components of Danshen, and the obtained dried Danshen medicinal materials have good appearance and appearance.

[0033] The present invention provides salvia miltiorrhiza prepared by the origin processing method described in the above technical scheme. In the present invention, the mass percentage of the index component of salvia miltiorrhiza is 4.90% to 8.96%, or 7.31% to 8.96%, or 7.54% to 8.96%. As an optional embodiment of the present invention, the mass percentage of the index component of salvia miltiorrhiza can be 4.90%, 5.35%, 6.35%, 6.82%, 7.20%, 7.31%, 7.54%, 8.27%, 8.36% or 8.96%. In the present invention, the index component includes salvianolic acid B and tanshinones; the tanshinones include cryptotanshinone, tanshinone I and tanshinone IIA. In the present invention, the mass percentage of salvianolic acid B of salvia miltiorrhiza can be 2.17% to 6.23%. As an optional embodiment of the present invention, the salvianolic acid B of the salvia miltiorrhiza can be 2.17%, 2.88%, 3.39%, 4.31%, 4.60%, 4.99%, 5.38%, 5.94%, 6.21% or 6.23%. In the present invention, the mass percentage of tanshinones in the salvia miltiorrhiza can be 1.83% to 3.00%. As an optional embodiment of the present invention, the mass percentage of tanshinones in the salvia miltiorrhiza can be 1.83%, 2.13%, 2.16%, 2.33%, 2.47%, 2.59%, 2.74%, 2.75%, 2.99% or 3.00%. In the present invention, the mass percentage of cryptotanshinone in the salvia miltiorrhiza can be 0.55% to 0.78%. As an optional embodiment of the present invention, the mass percentage of cryptotanshinone in the salvia miltiorrhiza can be 0.55%, 0.57%, 0.58%, 0.62%, 0.69%, 0.71%, 0.74%, 0.75% or 0.78%. In the present invention, the mass percentage of tanshinone I in the salvia miltiorrhiza can be 0.16% to 0.32%; as an optional embodiment of the present invention, the mass percentage of tanshinone I in the salvia miltiorrhiza can be 0.16%, 0.20%, 0.21%, 0.22%, 0.23%, 0.24%, 0.26%, 0.28%, 0.31% or 0.32%. In the present invention, the mass percentage of tanshinone ⅡA in the salvia miltiorrhiza can be 1.10% to 1.98%; as an optional embodiment of the present invention, the mass percentage of tanshinone ⅡA in the salvia miltiorrhiza can be 1.10%, 1.35%, 1.39%, 1.48%, 1.53%, 1.73%, 1.74%, 1.97% or 1.98%.

[0034] The present invention provides the application of the production area processing method described in the above technical solution in increasing the content of the index components of salvia miltiorrhiza. In the present invention, the index components of salvia miltiorrhiza include any one or more of salvianolic acid B, cryptotanshinone, tanshinone I and tanshinone II A.

[0035] In order to further illustrate the present invention, the technical solution provided by the present invention is described in detail below in conjunction with the accompanying drawings and embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0036] The salvia miltiorrhiza used in the following embodiments and experimental cases is from the same batch and the same specification, and the specifications of the salvia miltiorrhiza are the same.

[0037] Experimental Case 1

[0038] 1. Effects of different drying conditions on the index components of Salvia miltiorrhiza

[0039] Each test group took two-year-old fresh Danshen medicinal materials from Weifang, Shandong, removed the fibrous roots, washed with clean water, and sorted them. The drying temperatures of the oven were set at 40℃, 50℃, 60℃, 70℃, 80℃, 90℃, and 100℃, respectively. One test group corresponded to one temperature. After preheating, 100g of the treated medicinal materials were placed at each drying temperature for drying. The drying degree was ≤12.0% according to the current "Chinese Pharmacopoeia" regulations.

[0040] After drying, the index components of the dried Salvia miltiorrhiza in each experimental group were tested.

[0041] The detection method is:

[0042] Extraction method: Grind the dried Salvia miltiorrhiza into powder, take about 0.5g of the powder (pass through No. 3 sieve), accurately weigh, put it in a stoppered conical flask, accurately add 25mL of methanol, stopper it, weigh it, ultrasonically treat it (power 250W, frequency 33kHz) for 30min, cool it, weigh it again, make up the lost weight with methanol, shake it well, filter it, and you are done.

[0043] Chromatographic conditions: Elite Hypersil ODS column (250 mm × 4.6 mm, 5 μm); mobile phase: 0.1% formic acid solution (A)-acetonitrile (B); gradient elution program: 0-32.0 min 10.0%-38% B, 32.0-34.0 min 38.0%-59.0% B, 34.0-40.0 min 59.0%-59.0% B, 40.0-60.0 min 59.0%-90.0% A; flow rate: 1.0 mL min -1 ; Column temperature: 25℃; Injection volume: 10μL. Detection wavelength: Salvianolic acid B 286nm, Tanshinones 270nm.

[0044] The index components of the obtained Salvia miltiorrhiza were shown in Table 1 and Table 2. Figure 1 Among them, the HPLC chromatogram of the index components detected after Danshen was dried at 60°C is shown in Figure 5As shown in A. Salvia miltiorrhiza dried at 60℃ Figure 6 As shown in (a) in .

[0045] Table 1 Contents of index components in Salvia miltiorrhiza

[0046]

[0047]

[0048] As shown in Table 1, the contents of salvianolic acid B, cryptotanshinone, tanshinone I and tanshinone IIA were used as indicators to conduct a comprehensive evaluation of the dried samples (the contribution of each indicator was the same). The HPLC test results showed that at 40℃, the content of salvianolic acid B was 3.53%, the content of cryptotanshinone was 0.50%, the content of tanshinone I was 0.11%, the content of tanshinone II A was 0.71%, and the total content of tanshinones was 1.32%; at 50℃, the content of salvianolic acid B was 4.00%, the content of cryptotanshinone was 0.50%, the content of tanshinone I was 0.12%, the content of tanshinone II A was 0.70%, and the total content of tanshinones was 1.32%; at 60℃, the content of salvianolic acid B was 5.46%, the content of cryptotanshinone was 0.66%, the content of tanshinone I was 0.15%, the content of tanshinone II A was 0.97%, and the total content of tanshinones was 1.78%; at 70℃, the content of salvianolic acid B was 3.82%, the content of cryptotanshinone was 0.40%, the content of tanshinone I was 0.16%, the content of tanshinone II The content of salvianolic acid A at 80℃ was 2.56%, the content of cryptotanshinone was 0.53%, the content of tanshinone I was 0.13%, the content of tanshinone IIA was 0.83%, and the total content of tanshinones was 1.48%; the content of salvianolic acid B at 90℃ was 3.05%, the content of cryptotanshinone was 0.48%, the content of tanshinone I was 0.12%, the content of tanshinone IIA was 0.69%, and the total content of tanshinones was 1.29%; the content of salvianolic acid B at 100℃ was 4.70%, the content of cryptotanshinone was 0.46%, the content of tanshinone I was 0.15%, the content of tanshinone IIA was 0.67%, and the total content of tanshinones was 1.28%. The results showed that the content of the index components was higher when directly dried at 60℃ under various drying temperature conditions.

[0049] 2. Effects of sun-drying and shade-drying on the index components of Salvia miltiorrhiza

[0050] Take 100g of two-year-old fresh Danshen medicinal materials from Weifang, Shandong, remove the fibrous roots, wash with clean water, sort, and dry the processed medicinal materials in the sun. The sun-drying method is to expose the fresh medicinal materials to the sun until they are dry. At the same time, take 100g of fresh Danshen medicinal materials, remove the fibrous roots, wash with clean water, sort, and dry the processed medicinal materials in the shade. The shade drying method is to place the fresh medicinal materials in a dark, cool and ventilated place to dry naturally at room temperature. The degree of sun drying and shade drying is ≤12.0% according to the current "Chinese Pharmacopoeia".

[0051] The contents of index components in sun-dried and shade-dried Salvia miltiorrhiza are shown in Table 2 and Figure 1 shown. Figure 1 The sun in the Chinese character for sun-drying corresponds to sun-drying. Figure 1 The shade in the above sentence corresponds to shade drying. Figure 6 As shown in (b) in the figure; the appearance of the dried Salvia miltiorrhiza is as follows Figure 6 As shown in (c) in .

[0052] Table 2 Contents of index components in sun-dried and shade-dried Salvia miltiorrhiza

[0053]

[0054] As shown in Table 2, the dried samples were comprehensively evaluated with the contents of salvianolic acid B, cryptotanshinone, tanshinone I and tanshinone IIA as indicators (the contribution of each indicator is the same). The HPLC test results show that the content of salvianolic acid B in sun-dried salvia miltiorrhiza is 5.19%, the content of cryptotanshinone is 0.42%, the content of tanshinone I is 0.13%, the content of tanshinone IIA is 0.63%, and the total content of tanshinones is 1.18%; the content of salvianolic acid B in shade-dried salvia miltiorrhiza is 3.74%, the content of cryptotanshinone is 0.50%, the content of tanshinone I is 0.18%, the content of tanshinone IIA is 0.92%, and the total content of tanshinones is 1.60%. Compared with the above-mentioned method of directly drying the fresh medicinal materials at 60℃, the total content of salvianolic acid B and tanshinone components in the salvia miltiorrhiza medicinal materials obtained by this method is lower than that of direct drying at 60℃.

[0055] 3. Effects of sweating treatment on the index components of Salvia miltiorrhiza

[0056] Take 10kg of two-year-old fresh Salvia miltiorrhiza from Weifang, Shandong, remove the fibrous roots, wash with clean water, sort, pile up the processed fresh roots for 10 days, wrap them with plastic cloth to generate heat, spread the roots out for one night every two days, and cover them with plastic cloth again. When the inner part of the root turns purple-red, the sweating process ends and it is dried at 60℃. The degree of drying is ≤12.0% according to the current "Chinese Pharmacopoeia". The chromatogram of 60℃ drying after sweating is as follows Figure 5 As shown in B. The appearance of Danshen dried after sweating is as follows Figure 6 As shown in (d) in .

[0057] The dried samples were comprehensively evaluated with the properties of medicinal materials, salvianolic acid B, cryptotanshinone, tanshinone I and tanshinone IIA as indicators (the contribution of each indicator is the same). The HPLC test results show that the content of salvianolic acid B after sweating at 60°C is 3.46%, the content of cryptotanshinone is 0.66%, the content of tanshinone I is 0.30%, the content of tanshinone IIA is 1.82%, and the total content of tanshinones is 2.78%. In comparative example 1, the fresh medicinal materials were directly dried at 60°C. According to the total content of the index components, the content of salvianolic acid B in the salvia miltiorrhiza medicinal materials obtained by this method is low, and the tanshinone components are increased from 1.78% to 2.78% compared with direct drying at 60°C, but its process cycle is long, it is easy to mold, it is greatly affected by the weather, and the processing process is unstable and cumbersome. Figure 1 The sweating in the figure corresponds to the total content of the indicative components of the Salvia miltiorrhiza obtained by this treatment.

[0058] Example 1

[0059] Take two-year-old fresh Salvia miltiorrhiza medicinal materials from Weifang, Shandong, remove the fibrous roots, wash with clean water, sort, and obtain the treated Salvia miltiorrhiza. The treated Salvia miltiorrhiza was evenly and dispersedly spread in the middle of a constant temperature and humidity reaction box, and the parameters were set to 98% humidity, 40°C temperature, and the reaction time was 0.5h, 1h, 2h, and 3h for adversity treatment to obtain Salvia miltiorrhiza treated with adversity stress. The weight of the Salvia miltiorrhiza corresponding to each experimental group of reaction time was 100g. After the adversity stress treatment, the oven was preheated to 60°C and the Salvia miltiorrhiza treated with adversity stress was placed in it for drying. The degree of drying was ≤12.0% according to the current "Chinese Pharmacopoeia".

[0060] The contents of index components of Salvia miltiorrhiza treated with different stress times are shown in Table 3 and Figure 2 shown.

[0061] Table 3 Contents of index components in Salvia miltiorrhiza treated with different stress times

[0062]

[0063] As shown in Table 3, the contents of salvianolic acid B, cryptotanshinone, tanshinone I and tanshinone IIA were used as indicators to conduct a comprehensive evaluation of the dried samples (the contribution of each indicator is the same). The HPLC test results show that the content of salvianolic acid B was 4.99%, the content of cryptotanshinone was 0.57%, the content of tanshinone I was 0.16%, the content of tanshinone IIA was 1.10%, and the total content of tanshinones was 1.83% after 0.5h of constant temperature and humidity stress treatment; the content of salvianolic acid B was 4.60%, the content of cryptotanshinone was 0.62%, the content of tanshinone I was 0.24%, the content of tanshinone IIA was 1.73%, and the total content of tanshinones was 1.83% after 1h of constant temperature and humidity stress treatment. 2.59%; after 2h of constant temperature and humidity stress treatment, the content of salvianolic acid B was 4.31%, the content of cryptotanshinone was 0.71%, the content of tanshinone I was 0.32%, the content of tanshinone II A was 1.97%, and the total content of tanshinones was 3.00%; after 3h of constant temperature and humidity stress treatment, the content of salvianolic acid B was 3.39%, the content of cryptotanshinone was 0.75%, the content of tanshinone I was 0.26%, the content of tanshinone II A was 1.98%, and the total content of tanshinones was 2.99%. Compared with the direct drying of fresh medicinal materials at 60℃, the content of salvianolic acid B in the obtained Salvia miltiorrhiza medicinal materials under constant temperature and humidity stress treatment for 2h decreased from 5.46% to 4.31%, but the content of tanshinones increased from 1.78% to 3.00%, and the total content of index components was higher than that of direct drying at 60℃.

[0064] Example 2

[0065] Take two-year-old fresh Danshen medicinal materials from Weifang, Shandong, remove the fibrous roots, wash with clean water, and sort them. Evenly and dispersedly spread them in the middle of the constant temperature and humidity reaction box, set the parameters to 70%, 80%, 90%, and 98% respectively, and treat Danshen with adversity stress. Each humidity treatment is 100g of fresh medicinal materials. The temperature of adversity stress treatment is 40℃, and the reaction time of adversity stress treatment is 2h. After the treatment, the obtained medicinal materials are dried in an oven, and the drying temperature is set to 60℃. After preheating to 60℃, the medicinal materials treated with adversity stress are placed. The degree of drying is in accordance with the current "Chinese Pharmacopoeia" with a water content of ≤12.0%.

[0066] The contents of index components of Salvia miltiorrhiza treated with stress under different humidity conditions are shown in Table 4 and Figure 3 Among them, the HPLC chromatogram of the index component detection of Salvia miltiorrhiza after being subjected to stress treatment under 90% humidity, 40°C, and 2h and then dried at 60°C is shown in Figure 5 As shown in C, the appearance of the dried Salvia miltiorrhiza after adversity treatment is as follows Figure 6 As shown in (e) in .

[0067] Table 4 Contents of index components in Salvia miltiorrhiza treated with stress under different humidity conditions

[0068]

[0069] As shown in Table 4, the contents of salvianolic acid B, cryptotanshinone, tanshinone I and tanshinone IIA were used as indicators to conduct a comprehensive evaluation of the dried samples (the contribution of each indicator is the same). The HPLC test results show that the content of salvianolic acid B under constant temperature and humidity stress treatment at 70% humidity is 2.17%, the content of cryptotanshinone is 0.69%, the content of tanshinone I is 0.31%, the content of tanshinone IIA is 1.74%, and the total content of tanshinones is 2.74%; the content of salvianolic acid B under constant temperature and humidity stress treatment at 80% humidity is 6.23%, the content of cryptotanshinone is 0.55%, the content of tanshinone I is 0.23%, the content of tanshinone IIA is 1.35%, and the total content of tanshinones is 2. .13%; the content of salvianolic acid B, cryptotanshinone, tanshinone I, tanshinone II A, and total tanshinone content were 6.21%, 0.74%, 0.28%, 1.73%, and 2.75% respectively under constant temperature and humidity stress treatment at 90% humidity; the content of salvianolic acid B, cryptotanshinone, 0.71%, 0.32%, 1.97%, and 3.00% respectively under constant temperature and humidity stress treatment at 98% humidity. In the comparative test case, Salvia miltiorrhiza was directly dried at 60℃. At 90% humidity, the content of salvianolic acid increased significantly, from 5.46% to 6.21%, and the content of tanshinones increased from 1.78% to 2.75%. The total content was significantly better than the processing method in the test case, and the quality far exceeded the standard of the Chinese Pharmacopoeia.

[0070] Example 3

[0071] Take two-year-old fresh Danshen medicinal materials from Weifang, Shandong, remove the fibrous roots, wash with clean water, and sort. Evenly and dispersedly spread in the middle of the constant temperature and humidity reaction box, set the humidity parameter to 90%, and perform stress treatment at temperatures of 30℃, 40℃, 50℃, and 60℃, respectively. Treat 100g of fresh Danshen medicinal materials at each temperature, and the reaction time of stress treatment is 2h. After the stress treatment, the obtained medicinal materials are dried in an oven, and the drying temperature is set to 60℃. After preheating to 60℃, the medicinal materials treated with stress are placed in the oven. The degree of drying is ≤12.0% according to the current "Chinese Pharmacopoeia".

[0072] The contents of index components of Salvia miltiorrhiza treated with stress at different temperatures are shown in Table 5 and Figure 4 shown.

[0073] Table 5 Contents of index components in Salvia miltiorrhiza treated with stress at different temperatures

[0074]

[0075] As shown in Table 5, the contents of salvianolic acid B, cryptotanshinone, tanshinone I and tanshinone IIA were used as indicators to comprehensively evaluate the dried samples (the contribution of each indicator is the same). The HPLC test results showed that at 30℃, the content of salvianolic acid B was 5.94%, the content of cryptotanshinone was 0.58%, the content of tanshinone I was 0.22%, the content of tanshinone IIA was 1.53%, and the total content of tanshinones was 2.33%; at 40℃, the content of salvianolic acid B was 6.21%, the content of cryptotanshinone was 0.74%, the content of tanshinone I was 0.28%, the content of tanshinone IIA was 1.73%, and the total content of tanshinones was 2. .75%; at 50℃, the content of salvianolic acid B is 5.38%, the content of cryptotanshinone is 0.57%, the content of tanshinone I is 0.20%, the content of tanshinone II A is 1.39%, and the total content of tanshinones is 2.16%; at 60℃, the content of salvianolic acid B is 2.88%, the content of cryptotanshinone is 0.78%, the content of tanshinone I is 0.21%, the content of tanshinone II A is 1.48%, and the total content of tanshinones is 2.47%. In the comparative test case, the fresh medicinal materials are directly dried at 60℃ and treated with different adverse stresses in Example 1 and Example 2, with 90% humidity and 2h reaction time, the content of the index components of salvia miltiorrhiza at 40℃ is the most obvious, and the content of salvianolic acid B and tanshinone components is more abundant and average, which is in line with the traditional concept of Chinese medicine.

[0076] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A method for processing salvia miltiorrhiza at the place of origin, characterized in that: The following steps are involved: The salvia miltiorrhiza is subjected to stress treatment; the temperature of the stress treatment is 30 to 60° C.; the humidity of the stress treatment is 70% to 98%; the time of the stress treatment is 0.5 to 3 hours; The Salvia miltiorrhiza treated with adverse stress was dried.

2. The production site processing method according to claim 1, characterized in that: The temperature of the stress treatment is 30-50° C.; the humidity of the stress treatment is 80%-98%; and the time of the stress treatment is 1-2 hours.

3. The production site processing method according to claim 1, characterized in that: The temperature of the stress treatment is 30-40°C; the humidity of the stress treatment is 80%-90%; and the time of the stress treatment is 2 hours.

4. The production site processing method according to claim 1, characterized in that: The drying method includes any one or more of sun drying, shade drying and oven drying.

5. The production site processing method according to claim 4, characterized in that: The drying temperature is 40-100°C.

6. The production site processing method according to claim 4, characterized in that: The drying temperature is 50-70°C.

7. Salvia miltiorrhiza prepared by the origin processing method according to any one of claims 1 to 6.

8. The Salvia miltiorrhiza according to claim 7, characterized in that: The mass percentage of the index components of salvia miltiorrhiza is 4.90% to 8.96%; the index components include salvianolic acid B and tanshinones; the tanshinones include cryptotanshinone, tanshinone I and tanshinone II A.

9. Use of the origin processing method according to any one of claims 1 to 6 in increasing the content of index components in Salvia miltiorrhiza.

10. The use according to claim 9, characterized in that: The indicative components of salvia miltiorrhiza include any one or more of salvianolic acid B, cryptotanshinone, tanshinone I and tanshinone IIA.

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