Chinese herbal medicine composition for preventing and treating coccidiosis of northern Shaanxi white cashmere goats as well as preparation method and application of Chinese herbal medicine composition

Abstract

The invention provides a Chinese herbal medicine composition for preventing and treating coccidiosis of northern Shaanxi white cashmere goats and a preparation method and application thereof, and belongs to the technical field of Chinese herbal medicines. The Chinese herbal medicine composition comprises the following components in parts by weight: 10-20 parts of sweet wormwood, 8-16 parts of cape jasmine, 4-8 parts of rheum officinale, 8-16 parts of rhizoma atractylodis, 6-12 parts of radix bupleuri, 10-20 parts of oriental wormwood, 4-8 parts of astragalus membranaceus, 4-8 parts of liquorice, 5-12 parts of sophora alopecuroide and 4-9 parts of antifebrile dichroa. Natural Chinese herbal medicines such as artemisia apiacea and astragalus membranaceus produced in the northern Shaanxi region are developed and utilized, so that the problem of high incidence rate of coccidiosis of the northern Shaanxi white cashmere goats is solved, and the characteristics of medicinal value and no medicine residue of the artemisia apiacea and the astragalus membranaceus are utilized; when the feed additive is used for feeding the Shaanbei white cashmere goats, the problem of chemical medicine residues in animal products can be solved, the immunity of the goats is improved, and the coccidiosis morbidity of the goats is reduced.

Description

Technical Field

[0001] The invention belongs to the technical field of Chinese herbal medicines, and in particular relates to a Chinese herbal medicine composition for preventing and treating coccidiosis of white cashmere goats in northern Shaanxi, and a preparation method and application thereof. Background Art

[0002] In mutton sheep farming, the incidence of coccidiosis remains high. At present, the most effective means of controlling sheep coccidiosis is mainly to rely on chemical drugs for prevention and treatment, but with the long-term and irregular use of anticoccidial drugs, drug-resistant strains continue to emerge. Therefore, finding and developing safe and effective anticoccidial alternative drugs has become a research hotspot in the mutton sheep farming industry. How to scientifically utilize local Chinese herbal medicine resources to develop the Shaanxi white cashmere goat farming industry and reduce the incidence of coccidiosis and the residues of chemical drugs in livestock products is one of the current problems that need to be solved urgently. Summary of the invention

[0003] In view of this, the purpose of the present invention is to provide a Chinese herbal medicine composition for preventing and treating coccidiosis of white cashmere goats in northern Shaanxi, and a preparation method and application thereof. The present invention utilizes the medicinal value of Artemisia annua and Astragalus membranaceus and the characteristics of no drug residue to obtain a Chinese herbal medicine composition capable of preventing and treating coccidiosis of white cashmere goats in northern Shaanxi. The Chinese herbal medicine composition of the present invention is used to prevent and treat parasitic diseases of white cashmere goats in northern Shaanxi, while making full use of local excellent Chinese herbal medicine resources, effectively solving the problem of chemical drug residues in livestock products, reducing the incidence of coccidiosis, and improving breeding benefits.

[0004] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0005] The invention provides a Chinese herbal medicine composition for preventing and treating coccidiosis of white cashmere goats in northern Shaanxi. The composition comprises the following components in parts by weight: 10-20 parts of Artemisia annua, 8-16 parts of Gardenia jasminoides, 4-8 parts of Rhubarb, 8-16 parts of Atractylodes lancea, 6-12 parts of Bupleurum chinense, 10-20 parts of Artemisia capillaris, 4-8 parts of Astragalus membranaceus, 4-8 parts of Licorice, 5-12 parts of Sophora alopecuroides and 4-9 parts of Dichroa fescue.

[0006] Preferably, the following components are included in weight proportions: 11-14 parts of Artemisia annua, 8.5-12 parts of Gardenia jasminoides, 4.5-6 parts of Rhubarb, 8.5-12 parts of Atractylodes lancea, 6.5-8 parts of Bupleurum chinense, 11.5-14 parts of Capillaris ternata, 4.5-6 parts of Astragalus membranaceus, 4.5-6 parts of Licorice, 5.5-7 parts of Sophora alopecuroides, and 4.5-6 parts of Dichroa schizophreniae.

[0007] Preferably, the composition comprises the following components in parts by weight: 12 parts of Artemisia annua, 9 parts of Gardenia jasminoides, 5 parts of Rhubarb, 9 parts of Atractylodes lancea, 7 parts of Bupleurum chinense, 12 parts of Capillaris ternata, 5 parts of Astragalus membranaceus, 5 parts of Licorice, 6 parts of Sophora alopecuroides, and 5 parts of Dichroa serrata.

[0008] The present invention provides a method for preparing the Chinese herbal medicine composition. The method comprises the following steps: washing and drying the components, crushing the components, sieving the components, and collecting the components under the sieve to obtain the Chinese herbal medicine composition.

[0009] The present invention provides the use of the Chinese herbal medicine composition or the Chinese herbal medicine composition obtained according to the preparation method in preparing a preparation for preventing and treating coccidiosis of white cashmere goats in northern Shaanxi.

[0010] Preferably, the preparation method of the preparation is to grind the Chinese herbal composition and mix it with water to obtain the preparation; the mass volume ratio of the Chinese herbal composition to water is 15-85g:400mL.

[0011] The present invention also provides the use of the Chinese herbal medicine composition or the Chinese herbal medicine composition obtained according to the preparation method as an additive in preparing feed with the function of preventing and treating coccidiosis of white cashmere goats in northern Shaanxi.

[0012] Preferably, the additive is added in an amount of 0.5% to 7% of the feed mass.

[0013] The present invention also provides a feed for preventing and treating coccidiosis of white cashmere goats in northern Shaanxi, wherein the feed comprises the Chinese herbal medicine composition and a complete mixed diet; the mass ratio of the Chinese herbal medicine composition to the complete mixed diet is 1 to 7:100.

[0014] Preferably, the total mixed diet comprises the following components in percentage by mass: 25% to 35% corn, 4% to 8% millet, 4% to 8% sprayed corn husk, 10% to 14% soybean meal, 0.4% to 0.8% stone powder, 0.4% to 0.8% calcium hydrogen phosphate, 0.4% to 0.8% baking soda, 0.4% to 0.8% salt, 0.4% to 0.8% premix, 1% to 5% wheat bran, 20% to 30% peanut seedlings, and 10% to 20% corn stalks.

[0015] Compared with the prior art, the present invention has the following beneficial effects: the present invention develops and utilizes the natural Chinese herbal medicines Artemisia annua and Astragalus membranaceus produced in northern Shaanxi, which not only solves the problem of high incidence of coccidiosis in white cashmere goats in northern Shaanxi, but also utilizes their medicinal value and the characteristics of no drug residue. Feeding white cashmere goats in northern Shaanxi with the feed obtained by the present invention can solve the problem of chemical drug residues in livestock products, improve immunity, and reduce the incidence of coccidiosis. The prevention and control effect of coccidiosis in white cashmere goats in northern Shaanxi is better than that of existing Chinese herbal feed additives; compared with chemical drugs, it significantly reduces drug residues in livestock products and improves animal food safety; and at the same time promotes the sustainable development of the development and utilization of Chinese herbal medicine resources. DETAILED DESCRIPTION

[0016] The present invention provides a Chinese herbal medicine composition for preventing and treating coccidiosis of white cashmere goats in northern Shaanxi, comprising the following components in parts by weight:

[0017] 10-20 parts of Artemisia annua, preferably 11-14 parts, more preferably 12 parts, Artemisia annua has the effects of clearing away heat and relieving summer heat, relieving deficiency fever, and is mainly used to treat exogenous summer heat, yin deficiency fever, and parasitic diseases. It is the main drug in the present invention;

[0018] 10 to 20 parts of Artemisia capillaris, preferably 11.5 to 14 parts, more preferably 12 parts, Artemisia capillaris has the effects of clearing away heat and dampness, and relieving jaundice, and is the main drug in the present invention;

[0019] 8 to 16 parts of Gardenia, preferably 8.5 to 12 parts, more preferably 9 parts, Gardenia has the effect of clearing away dampness and heat in the triple energizer and making dampness and heat go out through urine, and is an adjuvant in the present invention;

[0020] 4 to 8 parts of rhubarb, preferably 4.5 to 6 parts, more preferably 5 parts, rhubarb can relieve stagnant heat and make damp heat go down through feces, and is an adjuvant drug in the present invention;

[0021] 5 to 12 parts of bitter beans, preferably 5.5 to 7 parts, more preferably 6 parts, bitter beans have the effects of clearing away heat and dampness, relieving pain, and killing insects, and are the ministerial drug in the present invention;

[0022] 4 to 9 parts of Radix Dichroae, preferably 4.5 to 6 parts, more preferably 5 parts, Radix Dichroae has the effects of killing insects, removing phlegm and eliminating accumulation, and is mainly used to treat coccidiosis and prevent old grass from turning, and is the ministerial drug in the present invention.

[0023] 8 to 16 parts of Atractylodes lancea, preferably 8.5 to 12 parts, more preferably 9 parts, Atractylodes lancea has the effects of drying dampness and strengthening the spleen, dispelling wind and cold, and improving eyesight, and is an adjuvant in the present invention;

[0024] 6 to 12 parts of Bupleurum, preferably 6.5 to 8 parts, more preferably 7 parts. Bupleurum has the effects of dispelling heat and tonifying the interior, raising Yang Qi, soothing the liver and relieving depression, and is an adjuvant in the present invention;

[0025] 4 to 8 parts of Astragalus, preferably 4.5 to 6 parts, more preferably 5 parts. Astragalus has the effects of invigorating Qi and raising Yang, consolidating the exterior and stopping sweating, supporting sores and promoting tissue regeneration, and promoting diuresis and reducing swelling. It is an adjuvant in the present invention;

[0026] 4-8 parts of licorice, preferably 4.5-6 parts, more preferably 5 parts, licorice has the effects of tonifying the middle and replenishing qi, moistening the lungs and relieving cough, clearing away heat and detoxifying, and alleviating the medicinal properties, and is used as a drug in the present invention;

[0027] In the present invention, Artemisia annua and Artemisia capillaris are used as the main drugs to eliminate dampness; Gardenia jasminoides, rhubarb, Sophora alopecuroides and Dichroa chinensis are used as the assistant drugs to clear away dampness and heat; Atractylodes lancea, Bupleurum chinense and Astragalus membranaceus are used as the adjuvant drugs to eliminate dampness and strengthen the spleen; Licorice is used as the guiding drug to harmonize the medicinal properties. Through the reasonable compatibility of the drugs, the therapeutic effect on goat coccidiosis is achieved together.

[0028] The present invention provides a method for preparing the Chinese herbal medicine composition. The method comprises the following steps: washing and drying the components, crushing the components, sieving the components, and collecting the components under the sieve to obtain the Chinese herbal medicine composition.

[0029] In the present invention, there is no special limitation on washing, drying and crushing, and conventional washing, drying and crushing methods can be used. The mesh number of the sieving is 60 to 100 meshes, preferably 70 to 90 meshes, and more preferably 80 meshes.

[0030] The present invention provides the use of the Chinese herbal medicine composition or the Chinese herbal medicine composition obtained according to the preparation method in preparing a preparation for preventing and treating coccidiosis of white cashmere goats in northern Shaanxi.

[0031] In the present invention, the preparation method of the preparation is to crush the Chinese herbal composition and mix it with water to obtain the preparation; the mass volume ratio of the Chinese herbal composition to water is 15-85g:400mL, preferably 50-80g:400mL, and further preferably 75g:400mL.

[0032] The present invention also provides the use of the Chinese herbal medicine composition or the Chinese herbal medicine composition obtained according to the preparation method as an additive in preparing feed with the function of preventing and treating coccidiosis of white cashmere goats in northern Shaanxi.

[0033] In the present invention, the additive is added in an amount of 0.5% to 7% of the feed mass, preferably 1% to 5%, more preferably 2% to 5%, and 5% is optimal.

[0034] The present invention also provides a feed for preventing and treating coccidiosis of white cashmere goats in northern Shaanxi, wherein the feed comprises the Chinese herbal medicine composition and a complete mixed diet; the mass ratio of the Chinese herbal medicine composition to the complete mixed diet is 1 to 7:100.

[0035] In the present invention, the mass ratio of the Chinese herbal medicine composition to the total mixed diet is preferably 3-6:100, more preferably 5:100, and the total mixed diet comprises the following components in mass percentage: 25%-35% corn, preferably 28%-32%, more preferably 30%, 4%-8% millet, preferably 5%-7%, more preferably 6%, 4%-8% sprayed corn husk, preferably 5%-7%, more preferably 6%, 10%-14% soybean meal, preferably 11%-13%, more preferably 12%, 0.4%-0.8% stone powder, preferably 0.5%-0.7%, more preferably 0.6%, 0.4%-0.0 .8%, preferably 0.5% to 0.7%, more preferably 0.6%, baking soda 0.4% to 0.8%, preferably 0.5% to 0.7%, more preferably 0.6%, salt 0.4% to 0.8%, preferably 0.5% to 0.7%, more preferably 0.6%, premix 0.4% to 0.8%, preferably 0.5% to 0.7%, more preferably 0.6%, wheat bran 1% to 5%, preferably 2% to 4%, more preferably 3%, peanut seedlings 20% to 30%, preferably 22% to 25%, more preferably 24%, corn straw 10% to 20%, preferably 15% to 18%, more preferably 16%.

[0036] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0037] The preparation method of the total mixed diet is as follows: 30% by mass of corn, 6% by mass of millet, 6% by mass of sprayed corn husk, 12% by mass of soybean meal, 0.6% by mass of stone powder, 0.6% by mass of calcium hydrogen phosphate, 0.6% by mass of baking soda, 0.6% by mass of salt, 0.6% by mass of premix, 3% by mass of wheat bran, 24% by mass of peanut vines, and 16% by mass of corn stalks are uniformly mixed to obtain the total mixed diet.

[0038] The complete mixed diets used in the subsequent examples were prepared in the above manner.

[0039] Example 1

[0040] Weigh 12g of washed and dried Artemisia annua, 9g of Gardenia jasminoides, 5g of Rhubarb, 9g of Atractylodes lancea, 7g of Bupleurum chinense, 12g of Artemisia capillaris, 5g of Astragalus membranaceus, 5g of Licorice root, 6g of Sophora alopecuroides, and 5g of Dichroa chinensis, crush them, and pass through an 80-mesh sieve to obtain a Chinese herbal medicine composition for preventing and treating coccidiosis in white cashmere goats in northern Shaanxi. The Chinese herbal medicine composition is mixed with 1500g of a complete mixed diet to obtain a feed for preventing and treating coccidiosis in white cashmere goats in northern Shaanxi, which is used for feeding one goat.

[0041] Example 2

[0042] Weigh 7g of washed and dried Artemisia annua, 6g of Gardenia jasminoides, 3g of Rhubarb, 6g of Atractylodes lancea, 4g of Bupleurum chinense, 7g of Artemisia capillaris, 3g of Astragalus membranaceus, 3g of Licorice root, 3g of Sophora alopecuroides, and 3g of Dichroa chinensis, crush them, and pass through an 80-mesh sieve to obtain a Chinese herbal medicine composition for preventing and treating coccidiosis in white cashmere goats in northern Shaanxi. The Chinese herbal medicine composition is mixed with 1500g of a complete mixed diet to obtain a feed for preventing and treating coccidiosis in white cashmere goats in northern Shaanxi, which is used for feeding one goat.

[0043] Example 3

[0044] Weigh 2.5g of washed and dried Artemisia annua, 1.9g of Gardenia jasminoides, 0.9g of Rhubarb, 1.9g of Atractylodes lancea, 1.4g of Bupleurum chinense, 2.3g of Artemisia capillaris, 0.9g of Astragalus membranaceus, 0.9g of Licorice root, 1.4g of Sophora alopecuroides, and 0.9g of Dichroa chinensis, crush them, and pass through an 80-mesh sieve to obtain a Chinese herbal medicine composition for preventing and treating coccidiosis in white cashmere goats in northern Shaanxi. The Chinese herbal medicine composition is mixed with 1500g of a complete mixed diet to obtain a feed for preventing and treating coccidiosis in white cashmere goats in northern Shaanxi, which is used for feeding one goat.

[0045] Example 4

[0046] Weigh 12g of washed and dried Artemisia annua, 9g of Gardenia jasminoides, 5g of Rhubarb, 9g of Atractylodes lancea, 7g of Bupleurum chinense, 12g of Capillaris lancea, 5g of Astragalus membranaceus, 5g of Licorice root, 6g of Sophora alopecuroides, and 5g of Dichroa chinensis, crush them, and pass through an 80-mesh sieve to obtain a Chinese herbal medicine composition for preventing and treating coccidiosis in white cashmere goats in northern Shaanxi. The Chinese herbal medicine composition is mixed with 400mL of water to obtain a medicament for preventing and treating coccidiosis in white cashmere goats in northern Shaanxi, which is used for gavage of one goat.

[0047] Example 5

[0048] Weigh 7g of washed and dried Artemisia annua, 6g of Gardenia jasminoides, 3g of Rhubarb, 6g of Atractylodes lancea, 4g of Bupleurum chinense, 7g of Capillaris scoparia, 3g of Astragalus, 3g of Licorice, 3g of Sophora alopecuroides, and 3g of Dichroa chinensis, crush them, and pass through an 80-mesh sieve to obtain a Chinese herbal medicine composition for preventing and treating coccidiosis in white cashmere goats in northern Shaanxi. The Chinese herbal medicine composition is mixed with 400mL of water to obtain a medicament for preventing and treating coccidiosis in white cashmere goats in northern Shaanxi, which is used for gavage of one goat.

[0049] Example 6

[0050] Weigh 2.5g of washed and dried Artemisia annua, 1.9g of Gardenia jasminoides, 0.9g of Rhubarb, 1.9g of Atractylodes lancea, 1.4g of Bupleurum chinense, 2.3g of Capillaris ternata, 0.9g of Astragalus membranaceus, 0.9g of Licorice, 1.4g of Sophora alopecuroides, and 0.9g of Dichroa chinensis, crush them, and pass through an 80-mesh sieve to obtain a Chinese herbal medicine composition for preventing and treating coccidiosis in white cashmere goats in northern Shaanxi. The Chinese herbal medicine composition is mixed with 400mL of water to obtain a medicament for preventing and treating coccidiosis in white cashmere goats in northern Shaanxi, which is used for gavage of one goat.

[0051] Experimental example

[0052] This experiment was conducted at Yulin Ruixing Agriculture and Animal Husbandry Technology Co., Ltd. 42 Shaanxi white cashmere goats with similar weight and age (8 months old) and all infected with coccidia were selected and randomly divided into 7 groups, 6 in each group. The control group was fed a total mixed diet (no medication), among which, the Experiment I (Example 3), II (Example 2), and III (Example 1) groups added 1%, 3%, and 5% of the mass of the total mixed diet to the total mixed diet; the Experiment IV (Example 6), V (Example 5), and VI (Example 4) groups were fed a total mixed diet and before feeding every morning, the original medicine of the Chinese herbal medicine composition of 1%, 3%, and 5% of the mass of the total mixed diet was respectively diluted into 400mL of liquid and administered orally.

[0053] The experimental diet was prepared as a total mixed ration according to the "Meat Sheep Feeding Standard" (NT / T816-2004) and production practice. The feeding experiment included a 7-day pre-trial period and a 14-day formal period.

[0054] The coccidia killing effect was tested before formal feeding, at 7d, 14d, and 7d, 14d, and 28d after drug withdrawal, and the results are shown in Table 1. The oocyst reduction rate and the oocyst relative reduction rate were tested at 7d, 14d, and 7d, 14d, and 28d after drug withdrawal, and the results are shown in Tables 2 and 3.

[0055] The evaluation of coccidia killing effect is based on the oocyst reduction rate and the relative oocyst reduction rate. The higher the two are, the better the killing effect.

[0056] The detection method for killing coccidia is: Oocyst count per gram of feces (OPG) detection method (McMaster method): take 1g of feces and place it in a beaker, add 30mL of saturated salt water, crush the feces with a glass rod and mix it evenly, filter and discard the feces residue, use a rubber-tipped dropper to absorb the suspension and add it to the counting plate, let it stand for 2 minutes, and count the number of eggs under a microscope. The calculation formula is:

[0057] OPG = [number of oocysts counted × (T / V)] / F

[0058] Note: T is the total volume of fecal suspension; V is the total volume of the sample added to the counting plate; F is the weight of feces.

[0059] Oocyst reduction rate = (number of oocysts per gram of feces before medication - number of oocysts per gram of feces after medication) / number of oocysts per gram of feces before medication × 100%

[0060] Relative reduction rate of oocysts = (number of oocysts per gram of feces in the control group - number of oocysts per gram of feces in the medication group) / number of oocysts per gram of feces in the control group × 100%

[0061] After 14 days of continuous medication, 5 mL of fresh blood was collected from all test sheep through the jugular vein before morning feeding. Serum was prepared by centrifugation at 3000 r / min for 10 min for subsequent determination of serum biochemical, antioxidant and immune indicators. The results are shown in Tables 4 to 6.

[0062] The detection method of serum biochemistry is: ALB, TP, GLO, Ca, GLU, BUN, P, CHOL, AMY, ALT, ALP, CK, TBIL, and CRE in the collected blood are measured using the method described in the operating instructions of the fully automatic blood biochemistry analyzer. The results are shown in Table 4.

[0063] Detection method of antioxidant indexes: The total antioxidant capacity of CAT catalase, SOD superoxide dismutase, GPX glutathione peroxidase, MDA malondialdehyde and T-AOC was determined by the method described in the kit instructions. The results are shown in Table 5. The kits were purchased from Jiangsu Addison Biotechnology Co., Ltd.

[0064] Detection method of immune indicators: IL-10 interleukin-10, IL-17 interleukin-17, IFN-γ interferon-γ, IgM immunoglobulin M, IgG immunoglobulin G, and IgA immunoglobulin A were measured using the method described in the kit instructions. The results are shown in Table 6. The kits were purchased from Jiangsu Jingmei Biotechnology Co., Ltd.

[0065] Table 1 Effects of different treatment groups on infection intensity before and after treatment

[0066]

[0067] Table 2 Oocyst reduction rate in different treatment groups

[0068]

[0069]

[0070] Table 3 Relative reduction rate of oocysts in different treatment groups

[0071]

[0072] Table 4 Serum biochemical parameters in different treatment groups

[0073]

[0074]

[0075] Note: The unit of ALB, TP, GLO is g / L; the unit of Ca, GLU, BUN, P, CHOL is mmol / L; the unit of AMY, ALT, ALP, CK is U / L; the unit of TBIL, CRE is μmol / L.

[0076] Table 5 Antioxidant indexes of different treatment groups

[0077]

[0078]

[0079] Note: CAT is catalase; SOD is superoxide dismutase; GPX is glutathione peroxidase; MDA is malondialdehyde; T-AOC is total antioxidant capacity.

[0080] Table 6 Cytokines in different treatment groups

[0081]

[0082] Note: IL-10 interleukin-10; IL-17 interleukin-17; IFN-γ interferon-γ; IgM immunoglobulin M; IgG immunoglobulin G; IgA immunoglobulin A.

[0083] Infection intensity: can reflect the number of coccidia oocysts per gram of feces of the test animals; as shown in Table 1, the number of coccidia in each test group of animals at each stage after medication was reduced to varying degrees, indicating that the Chinese herbal medicine composition obtained by the present invention has a preventive and therapeutic effect on coccidiosis of Shaanxi white cashmere goats.

[0084] Oocyst reduction rate: can reflect the percentage of the number of coccidia oocysts in the feces of the test animals after medication compared with before medication; the relative oocyst reduction rate can reflect the percentage of the number of coccidia oocysts in the feces of the test animals after medication compared with the control group; as can be seen from Tables 2 to 3, the use of the Chinese herbal medicine composition of the present invention can kill the coccidia oocysts in the bodies of the northern Shaanxi white cashmere goats, thereby having a preventive and therapeutic effect on the northern Shaanxi white cashmere goats.

[0085] The reference range of ALB in serum biochemical indicators is 21-37. When it is elevated, it is common in dehydration, while when it is reduced, it is common in malnutrition, impaired liver function, massive bleeding, parasites or intestinal wasting diseases. The reference range of TP is 57-81. When it is elevated, it is common in dehydration and increased immunoglobulins. When it is reduced, it is common in liver, kidney, gastrointestinal diseases, malnutrition, and chronic inflammation. The reference range of GLO is 20-60. When it is elevated, it indicates chronic inflammation and infection. A / G reduction is common in liver diseases (such as chronic hepatitis, cirrhosis, liver cancer, liver damage, etc.), nephrotic syndrome, acute and chronic nephritis, infection or malnutrition. The reference range of Ca is 2.23-2.93. When it is elevated, it is common in hypercalcemia, primary hyperthyroidism, and multiple myeloma. When it is reduced, it is common in hypocalcemia and small intestinal absorption disorders. The reference range of GLU is 2.77-5.17. When it is elevated, it is common in lack of insulin secretion, and when it is reduced, it is common in malnutrition, indigestion, and chronic anemia. BUN reference range 1.8 ~ 7.1, increased common in kidney disease, excessive protein decomposition, high protein intake: decreased common in liver failure, insufficient protein absorption is related to calcium ion metabolism, increased common in kidney disease, growth period, bone healing period; decreased common in unbalanced diet, hyperthyroidism. P reference range 1.29 ~ 2.87, related to calcium ion metabolism, increased common in kidney disease, growth period, bone healing period; decreased common in unbalanced diet, hyperthyroidism. AMY reference range 141 ~ 540, increased common in pancreatitis, kidney disease, intestinal mucosal disease; decreased common in pancreatic necrosis caused by pancreatic duct obstruction. CHOL reference range 1.05 ~ 2.12, increased common in liver and kidney disease, endocrine abnormalities; decreased common in excessive exercise, malnutrition, progressive liver disease, hyperthyroidism. ALT reference range 5 ~ 28, increased common in primary hepatocellular and hepatobiliary system diseases, metabolic disorders, secondary liver diseases, drug effects. The reference range of TBIL is 0-7. Increased values ​​are common in liver and gallbladder dysfunction; decreased values ​​are common in anemia. The reference range of ALP is 26.9-283.3. Increased values ​​are common in liver and gallbladder diseases, malignant tumors, and drug effects; decreased values ​​are common in hypothyroidism and zinc deficiency. The reference range of CRE is 53-159. Increased values ​​are common in kidney diseases; decreased values ​​are common in malnutrition and muscle atrophy. Increased values ​​of BUN / CRE are often seen in fever, dehydration, gastrointestinal bleeding, heart failure, hepatorenal syndrome, urinary tract obstruction, and high-protein diet; decreased values ​​are often seen in hunger, low-protein diet, combined with severe liver failure, and the use of diuretics. The reference range of CK is 8-510. Increased values ​​are often seen in myocardial infarction or muscle atrophy.

[0086] Among the antioxidant indicators, the CAT value is higher than that of the control group, which means that the experimental animals are actively resisting coccidiosis infection after taking the medicine, and trying to maintain the stability of the internal environment by enhancing antioxidant capacity. The relationship between SOD activity and the treatment of coccidiosis with Chinese herbal medicine is mainly reflected in the repair of antioxidant damage: coccidiosis infection can cause the animal body to produce a large number of free radicals, causing oxidative stress damage. Some Chinese herbal medicines are rich in antioxidant ingredients, which can increase SOD activity, enhance the body's ability to scavenge free radicals, reduce oxidative damage, and promote body recovery. For example, the astragalus in the present invention can increase SOD activity and reduce the oxidative stress caused by coccidiosis infection. In summary, when using the Chinese herbal composition of the present invention to treat coccidiosis, the increase in SOD activity value is a positive manifestation of the body's self-protection, immune regulation and functional recovery under the action of the drug, reflecting that the Chinese herbal composition of the present invention has played a certain role in promoting the treatment of coccidiosis and the recovery of the body. In the process of using the Chinese herbal medicine composition of the present invention to treat coccidiosis, the increase in the value of GSH-Px enzyme activity is an important manifestation of the body's self-protection and recovery under the action of the drug, reflecting the positive effect of the Chinese herbal medicine composition of the present invention on the treatment of coccidiosis. After the body is infected with coccidia, immune dysfunction will aggravate oxidative stress and tissue damage, leading to an increase in MDA. The astragalus in the present invention can enhance the body's immune function, enable immune cells to better remove coccidia, reduce inflammatory reactions, indirectly reduce oxidative stress and the generation of MDA, and promote the reduction of MDA values. In summary, when the Chinese herbal medicine composition of the present invention is used to treat coccidiosis, the reduction in MDA values ​​is the result of the Chinese herbal medicine playing the role of anti-oxidation and immune regulation, reflecting the reduction of oxidative damage to the body and the improvement of the condition. In the process of using the Chinese herbal medicine composition of the present invention to treat coccidiosis, the increase in T-AOC values ​​is a comprehensive manifestation of the body's enhanced antioxidant defense ability, improved immune function and improved overall state under the action of Chinese herbal medicine, reflecting the positive effect of the Chinese herbal medicine composition of the present invention on the treatment of coccidiosis.

[0087] Among the cytokine indicators, in general, coccidia infection can trigger an inflammatory response in the body, leading to the release of cytokines such as IL-10. IL-10 is an anti-inflammatory cytokine, and proper secretion helps to reduce inflammatory damage, but excessive secretion can inhibit the body's immune response, which is not conducive to the removal of coccidia. The Chinese herbal composition of the present invention can reduce the secretion of IL-10 to a reasonable level by regulating the body's immune balance, which can not only avoid excessive inflammatory reactions, but also ensure that the body has sufficient immune ability to remove coccidia. After the body is infected with coccidia, the function of immune cells will change, affecting the secretion of IL-10. The astragalus in the present invention can enhance the phagocytic function of macrophages, make the cytokines secreted by macrophages more balanced, reduce the excessive secretion of IL-10, so that the body's immune response is more conducive to the removal of coccidia and promote recovery of the disease. When the Chinese herbal composition of the present invention is used to treat coccidiosis, the reduction of IL-10 values ​​is a manifestation of the reasonable regulation of the body's immune and inflammatory responses, reflecting that the Chinese herbal composition of the present invention has played a positive role in the treatment of coccidiosis, but the specific situation still needs to be combined with other immune indicators and clinical symptoms for comprehensive judgment.

[0088] IL-17 is a pro-inflammatory cytokine that plays an important role in the body's resistance to coccidia infection. It can recruit immune cells such as neutrophils to the site of infection, enhancing the body's phagocytosis and killing of coccidia. Some Chinese herbal medicines can activate immune cells and promote the secretion of IL-17, thereby enhancing the body's immune defense capabilities. Although IL-17 is a pro-inflammatory factor, it can help the body control the inflammatory response caused by coccidia infection to a certain extent. Coccidia infection can cause damage to tissues such as the intestines and cause inflammation. The Chinese herbal medicine composition of the present invention regulates the expression of IL-17 to keep the inflammatory response in a moderate state, which is beneficial for clearing coccidia and avoiding excessive inflammation from causing damage to the body. In the process of using the Chinese herbal medicine composition of the present invention to treat coccidiosis, the increase in IL-17 values ​​is a manifestation that the body's immune function is activated and the inflammatory response is reasonably regulated, which reflects that the Chinese herbal medicine composition of the present invention plays an anti-coccidiosis role by regulating immune-related factors.

[0089] IFN-γ can inhibit the growth and reproduction of coccidia by activating immune cells to produce substances such as nitric oxide. The Chinese herbal composition of the present invention can induce the body to produce IFN-γ, thereby indirectly exerting an anti-coccidial effect. When the Chinese herbal composition of the present invention is used to treat coccidiosis, the increase in IFN-γ value is a manifestation of enhanced immune function and reasonable regulation of immune response, which helps the body to eliminate coccidia and alleviate infection symptoms, reflecting the positive role of the Chinese herbal composition of the present invention in anti-coccidiosis.

[0090] IgM is the earliest antibody produced in the body's primary immune response. After coccidia infect the body, the immune system will recognize coccidia antigens and initiate an immune response. The astragalus in the present invention can enhance the body's immune recognition ability, promote B cell differentiation and proliferation, thereby accelerating the production of IgM and increasing the IgM value. It helps the body to quickly eliminate coccidia in the early stages of infection. In the process of using the Chinese herbal composition of the present invention to treat coccidiosis, the increase in IgM value is usually a manifestation of enhanced immune function and improved immune environment of the body, reflecting that the Chinese herbal composition of the present invention promotes the body to produce an immune response through multiple pathways to fight against coccidia infection.

[0091] IgG is the main antibody of the body against infection and plays an important role in the secondary immune response. The effective ingredients in the Chinese herbal composition of the present invention can stimulate the body's immune system, promote the proliferation and differentiation of B cells into plasma cells, thereby producing more IgG. Improve the body's immune defense ability against coccidia. After infection with coccidia, the body's immune system will form immune memory. The Chinese herbal composition of the present invention helps to strengthen this immune memory, so that the body can produce IgG faster and more strongly when it contacts the coccidia antigen again. It can regulate the function of immune cells and enhance the activity of immune memory cells. When coccidia invade again, it can quickly induce the production of a large amount of IgG, remove coccidia in time, and alleviate the symptoms of infection. The Chinese herbal composition of the present invention has an immunomodulatory effect, which can make the body's immune response in a balanced state, which is conducive to the production and function of IgG. The Chinese herbal composition of the present invention can regulate the function of T cells and B cells, balance cellular immunity and humoral immunity, create a good immune environment for the production of IgG, increase the secretion of IgG and better exert its immune effect, thereby effectively fighting coccidia infection. When the Chinese herbal medicine composition of the present invention is used to treat coccidiosis, the increase in IgG value is a comprehensive manifestation of the body's enhanced immune function, enhanced immune memory and immune balance regulation, which helps the body to more effectively resist coccidia infection and promote recovery from the disease.

[0092] The increase in IgA values ​​is closely related to the treatment of coccidiosis by the Chinese herbal composition of the present invention. Coccidia mainly infect mucosal tissues such as the intestines, and IgA is an important component of mucosal immunity. The Chinese herbal composition of the present invention can enhance the immune function of the intestinal mucosa and promote the secretion of IgA by the intestinal mucosa-associated lymphoid tissue. The astragalus in the Chinese herbal composition of the present invention can regulate the function of intestinal immune cells, promote the differentiation of B cells in mucosal immune tissues such as lymph nodes into plasma cells, and then increase the secretion of IgA, forming an immune barrier on the surface of the intestinal mucosa to prevent coccidia from invading intestinal epithelial cells. The Chinese herbal composition of the present invention may enable the immune system to produce IgA more effectively against coccidia antigens by improving the body's ability to recognize antigens. At the same time, IgA can bind to coccidia antigens to form immune complexes, which are then engulfed and cleared by phagocytes. The effective ingredients in the Chinese herbal composition of the present invention can enhance the activity of immune cells, improve their ability to absorb and process antigens, and prompt the body to produce more IgA against coccidia antigens, thereby accelerating the removal of coccidia and their antigens and reducing the damage of coccidia to the body. After the body is infected with coccidia, the immune function will be disordered. The Chinese herbal medicine composition of the present invention can regulate the overall immune balance, which is conducive to the normal secretion and function of IgA. The astragalus in the Chinese herbal medicine composition of the present invention has an immunomodulatory effect. It can regulate the functions of immune cells such as T cells and B cells, maintain the interaction balance between immune cells, and provide a stable immune environment for the production and function of IgA, so that IgA can better play its immune protection role when fighting against coccidia infection, thereby leading to an increase in IgA values.

[0093] In the process of treating coccidiosis with the Chinese herbal medicine composition of the present invention, the increase in IgA value is a manifestation of enhanced mucosal immune function, improved antigen clearance ability and regulated immune balance, which helps the body resist coccidia infection and protect mucosal tissues such as the intestine from coccidia.

[0094] In summary, it can be seen from the serum biochemical indices, antioxidant indices and cytokines in Tables 4 to 6 that adding the Chinese herbal composition of the present invention to feed can improve the immunity and disease resistance of animals, and as the amount of addition increases, the immunity of animals gradually improves.

[0095] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A Chinese herbal medicine composition for preventing and treating coccidiosis in white cashmere goats in northern Shaanxi, characterized in that: The invention comprises the following components in parts by weight: 10-20 parts of artemisia annua, 8-16 parts of gardenia, 4-8 parts of rhubarb, 8-16 parts of atractylodes, 6-12 parts of bupleurum, 10-20 parts of capillaris, 4-8 parts of astragalus, 4-8 parts of liquorice, 5-12 parts of sophora alopecuroides and 4-9 parts of radix scutellariae.

2. The Chinese herbal medicine composition according to claim 1, characterized in that: The invention comprises the following components in parts by weight: 11-14 parts of Artemisia annua, 8.5-12 parts of Gardenia jasminoides, 4.5-6 parts of Rhubarb, 8.5-12 parts of Atractylodes lancea, 6.5-8 parts of Bupleurum chinense, 11.5-14 parts of Artemisia capillaris, 4.5-6 parts of Astragalus membranaceus, 4.5-6 parts of Licorice root, 5.5-7 parts of Sophora alopecuroides and 4.5-6 parts of Dichroa chinensis.

3. The Chinese herbal medicine composition according to claim 1 or 2, characterized in that: The invention comprises the following components in parts by weight: 12 parts of sweet artemisia annua, 9 parts of gardenia jasminoides, 5 parts of rhubarb, 9 parts of atractylodes, 7 parts of bupleurum, 12 parts of capillaris, 5 parts of astragalus, 5 parts of liquorice, 6 parts of sophora alopecuroides and 5 parts of radix changshanii.

4. The method for preparing the Chinese herbal medicine composition according to any one of claims 1 to 3, characterized in that: The preparation method comprises the following steps: washing and drying the components, crushing the components, sieving the components, and collecting the components under the sieve to obtain the Chinese herbal medicine composition.

5. Use of the Chinese herbal medicine composition according to any one of claims 1 to 3 or the Chinese herbal medicine composition obtained by the preparation method according to claim 4 in the preparation of a preparation for preventing and treating coccidiosis in white cashmere goats in northern Shaanxi.

6. The use according to claim 5, characterized in that: The preparation method of the preparation is to grind the Chinese herbal medicine composition and mix it with water to obtain the preparation; the mass volume ratio of the Chinese herbal medicine composition to water is 15-85g:400mL.

7. Use of the Chinese herbal medicine composition according to any one of claims 1 to 3 or the Chinese herbal medicine composition obtained by the preparation method according to claim 4 as an additive in preparing feed with the function of preventing and treating coccidiosis in Shaanxi white cashmere goats.

8. The use according to claim 7, characterized in that: The additive is added in an amount of 0.5% to 7% of the feed mass.

9. A feed for preventing and treating coccidiosis in white cashmere goats in northern Shaanxi, characterized in that: The feed comprises the Chinese herbal medicine composition according to any one of claims 1 to 3 and a total mixed diet; the mass ratio of the Chinese herbal medicine composition to the total mixed diet is 1 to 7:

100.

10. The feed according to claim 9, characterized in that The total mixed diet comprises the following components in percentage by mass: 25% to 35% corn, 4% to 8% millet, 4% to 8% sprayed corn husk, 10% to 14% soybean meal, 0.4% to 0.8% stone powder, 0.4% to 0.8% calcium hydrogen phosphate, 0.4% to 0.8% baking soda, 0.4% to 0.8% salt, 0.4% to 0.8% premix, 1% to 5% wheat bran, 20% to 30% peanut seedlings, and 10% to 20% corn stalks.

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