Antibacterial composition as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of antibiosis, and discloses an antibacterial composition as well as a preparation method and application thereof. The antibacterial composition comprises a cinnamon extract, an aloe extract, a pseudo-ginseng extract and a scutellaria baicalensis extract. According to the antibacterial composition disclosed by the invention, the cortex cinnamomi extract, the aloe extract, the radix notoginseng extract and the radix scutellariae extract are mutually matched for use, so that a synergistic antibacterial effect is generated, and an unexpected technical effect is achieved in the aspects of resisting staphylococcus aureus and escherichia coli.

Description

Technical Field

[0001] The invention belongs to the field of antibacterial technology, and particularly relates to an antibacterial composition and a preparation method and application thereof. Background Art

[0002] Bacteria and viruses are everywhere in our lives, and there are many types of bacteria and viruses that are harmful to human health, which pose a threat to human health. In order to improve the level of human health, various antibacterial products have emerged. These antibacterial products can be roughly divided into two categories, one is antibacterial compounds, and the other is natural antibacterial compositions. Among them, antibacterial compounds are often prone to obvious side effects or a single antibacterial type. Natural antibacterial compositions have few side effects and also have certain broad-spectrum antibacterial properties, but they often have poor antibacterial effects against specific strains. For example, the antibacterial effects against Staphylococcus aureus and Escherichia coli need to be improved.

[0003] Therefore, there is an urgent need to provide a new antibacterial composition that has a good antibacterial effect on Staphylococcus aureus and Escherichia coli, and the raw material components of the antibacterial composition are derived from natural substances and have little side effects. Summary of the invention

[0004] The present invention aims to solve at least one of the technical problems existing in the above-mentioned prior art. To this end, the present invention provides an antibacterial composition and a preparation method and application thereof. The antibacterial composition of the present invention has good antibacterial properties, especially significant antibacterial effects on Staphylococcus aureus and Escherichia coli. Moreover, the raw material components of the antibacterial composition are derived from natural substances, and the side effects are small.

[0005] A first aspect of the present invention provides an antimicrobial composition.

[0006] Specifically, an antibacterial composition includes cinnamon extract, aloe extract, Panax notoginseng extract, and Scutellaria baicalensis extract.

[0007] Preferably, the antibacterial composition comprises 5-20 parts of cinnamon extract, 3-15 parts of aloe extract, 1-5 parts of Panax notoginseng extract, and 5-15 parts of Scutellaria baicalensis extract by weight. The appropriate dosage of each component helps to improve the antibacterial effect of the antibacterial composition.

[0008] Further preferably, the antibacterial composition comprises, by weight, 8-15 parts of cinnamon extract, 5-12 parts of aloe extract, 2-5 parts of Panax notoginseng extract, and 8-12 parts of Scutellaria baicalensis extract.

[0009] Preferably, the method for preparing the cinnamon extract comprises the following steps:

[0010] Take cinnamon, crush it, mix it with water, heat it to boil in a distillation device, collect the product, and obtain cinnamon extract.

[0011] Preferably, the weight ratio of cinnamon to water is 1:(5-10), more preferably 1:(5-6).

[0012] Preferably, the heating and boiling time is 1-7 hours, more preferably 2-5 hours.

[0013] Preferably, after the product is collected, it is dried, for example, using anhydrous sodium sulfate.

[0014] Preferably, the preparation method of the aloe extract comprises the following steps:

[0015] Take aloe vera, crush it, add ethanol to soak it, then use microwave radiation to extract it, then filter it to obtain an extract a and a filter residue, and recover the solvent to obtain a concentrated solution a;

[0016] The filter residue is soaked in a water-saturated n-butanol solution, extracted by ultrasonication, filtered to obtain an extract solution b, and the solvent is recovered to obtain a concentrated solution b;

[0017] The concentrated solution a and the concentrated solution b are mixed and dried to finally obtain the aloe vera extract.

[0018] Preferably, the weight ratio of aloe vera to ethanol is 1:(5-15), more preferably 1:(8-12).

[0019] Preferably, the temperature of the ethanol is 40-60°C.

[0020] Preferably, the temperature for soaking in ethanol is 40-60° C., and the soaking time for soaking in ethanol is 2-5 hours.

[0021] Preferably, during the microwave radiation extraction process, the power of the microwave radiation is 150-200 W, and the microwave radiation time is 3-8 minutes.

[0022] Preferably, the weight ratio of the filter residue to the water-saturated n-butanol solution is 1:(3-12), and more preferably 1:(8-12).

[0023] Preferably, the filter residue is soaked in a water-saturated n-butanol solution at a temperature of 35-60° C. and a soaking time of 0.5-1 hour.

[0024] Preferably, the ultrasonic extraction process is to first extract at 40-60KHz for 1-3 minutes, then extract at 80-90KHz for 5-10 minutes, then extract at 40-60KHz for 4-6 minutes, and then extract at 80-90KHz for 5-10 minutes. The aloe extract extracted under this process, combined with other components, can significantly improve the antibacterial effect of the antibacterial composition of the present invention on Staphylococcus aureus and Escherichia coli.

[0025] Preferably, the method for preparing the Panax notoginseng extract comprises the following steps:

[0026] Take Panax notoginseng, crush it, mix it with water, heat it to boiling in a distillation device, collect the product, and obtain Panax notoginseng extract.

[0027] Preferably, the weight ratio of Panax notoginseng to water is 1:(4-10), and more preferably 1:(4-6).

[0028] Preferably, the heating and boiling time is 4-7 hours, more preferably 4-5 hours.

[0029] Preferably, after the product is collected, it is dried, for example, using anhydrous sodium sulfate.

[0030] Preferably, the method for preparing the scutellaria baicalensis extract comprises the following steps:

[0031] Take Scutellaria baicalensis, crush it, soak it in water, and then extract it by microwave radiation, the power of microwave radiation is 180-200W, the time of microwave radiation is 3-8 minutes, then filter to obtain extract 1 and filter residue, concentrate extract 1 to obtain concentrated solution 1;

[0032] The filter residue is soaked in ethanol and ultrasonically extracted, wherein the ultrasonic extraction process is firstly to extract at 20-30KHz for 5-10 minutes, then to extract at 85-95KHz for 5-10 minutes, then to extract at 20-30KHz for 5-10 minutes, then to extract at 85-95KHz for 5-10 minutes, and then to filter to obtain extract 2, and to concentrate extract 2 to obtain concentrate 2;

[0033] The concentrated solution 1 and the concentrated solution 2 are mixed and dried to finally obtain the scutellaria baicalensis extract. The aloe extract extracted under this process, combined with other components, can make the antibacterial composition of the present invention significantly improve the antibacterial effect against Staphylococcus aureus and Escherichia coli.

[0034] Preferably, the weight ratio of Scutellaria baicalensis to water is 1:(3-18), more preferably 1:(8-15).

[0035] Preferably, the temperature of the water is 70-80°C.

[0036] Preferably, the temperature for soaking in water is 70-80° C., and the soaking time for soaking in water is 1-3 hours.

[0037] Preferably, the weight ratio of the filter residue to ethanol is 1:(6-15), more preferably 1:(8-14).

[0038] Preferably, the filter residue is soaked in ethanol at a temperature of 35-60° C. and a soaking time of 0.5-1.5 hours.

[0039] Preferably, the antibacterial composition further comprises 0.1-5 parts of Phellodendron chinense extract. The Phellodendron chinense extract is prepared by conventional water extraction method. The addition of Phellodendron chinense extract helps to further enhance the antibacterial effect of the antibacterial composition.

[0040] The second aspect of the present invention provides a method for preparing an antibacterial composition.

[0041] Specifically, a method for preparing an antibacterial composition comprises the following steps:

[0042] The components are mixed to obtain the antibacterial composition.

[0043] The third aspect of the present invention provides a use of an antibacterial composition.

[0044] Application of the antibacterial composition of the present invention in the preparation of antibacterial products.

[0045] Preferably, the antibacterial product includes a medical drug or a medical device.

[0046] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0047] (1) The antibacterial composition of the present invention produces a synergistic antibacterial effect by using cinnamon extract, aloe extract, Panax notoginseng extract and Scutellaria baicalensis extract in combination with each other, and achieves unexpected technical effects in terms of anti-Staphylococcus aureus and Escherichia coli.

[0048] (2) The special extraction method of the aloe vera extract and the scutellaria baicalensis extract of the present invention (microwave and ultrasonic extraction are performed successively, and the ultrasonic extraction is performed by alternating high and low frequencies, which is conducive to the full extraction of effective antibacterial components) can fully obtain the effective antibacterial molecules in aloe vera and scutellaria baicalensis, and then combine them with the effective ingredients in cinnamon extract and Panax notoginseng extract, thereby significantly improving the antibacterial effect against Staphylococcus aureus and Escherichia coli. DETAILED DESCRIPTION

[0049] In order to make the technical scheme of the present invention more clearly understood by those skilled in the art, the following embodiments are listed for illustration. It should be pointed out that the following embodiments do not limit the protection scope of the present invention.

[0050] Unless otherwise specified, the raw materials, reagents or devices used in the following examples can be obtained from conventional commercial sources or by existing known methods.

[0051] Example 1

[0052] An antibacterial composition comprises, by weight, 8 parts of cinnamon extract, 5 parts of aloe extract, 2 parts of notoginseng extract and 7 parts of scutellaria baicalensis extract.

[0053] The preparation method of cinnamon extract comprises the following steps:

[0054] Take cinnamon, crush it, mix it with water, heat it to boil in a distillation device, collect the product, and obtain cinnamon extract;

[0055] The weight ratio of cinnamon to water is 1:9, and the boiling time is 5 hours;

[0056] After the product was collected, it was dried over anhydrous sodium sulfate.

[0057] The preparation method of aloe extract comprises the following steps:

[0058] Take aloe vera, crush it, add ethanol to soak it, the weight ratio of aloe vera to ethanol is 1:10, the temperature of adding ethanol to soak it is 60°C, the time of adding ethanol to soak it is 2 hours, then use microwave radiation to extract it, the power of microwave radiation is 200W, the time of microwave radiation is 5 minutes, then filter to obtain extract a and filter residue, and recover the solvent to obtain concentrated solution a;

[0059] The filter residue is soaked in a water-saturated n-butanol solution, the weight ratio of the filter residue to the water-saturated n-butanol solution is 1:8, and ultrasonic extraction is performed. The ultrasonic extraction process is firstly extracted at 50KHz for 2 minutes, then extracted at 80KHz for 6 minutes, then extracted at 50KHz for 4 minutes, and then extracted at 80KHz for 8 minutes, and then filtered to obtain an extract b, and the solvent is recovered to obtain a concentrated solution b;

[0060] The concentrated solution a and the concentrated solution b are mixed and dried to finally obtain the aloe vera extract.

[0061] The preparation method of Panax notoginseng extract comprises the following steps:

[0062] Take Panax notoginseng, crush it, and then mix it with water, the weight ratio of Panax notoginseng to water is 1:5, heat it to boiling in a distillation device, the boiling time is 5 hours, collect the product, and dry it with anhydrous sodium sulfate to obtain Panax notoginseng extract.

[0063] The preparation method of the scutellaria baicalensis extract comprises the following steps:

[0064] Take scutellaria baicalensis, crush it, soak it in water (the soaking temperature is 70°C, and the soaking time is 1.5 hours), the weight ratio of scutellaria baicalensis to water is 1:12, then use microwave radiation extraction, the microwave radiation power is 20W, the microwave radiation time is 4 minutes, then filter to obtain extract 1 and filter residue, concentrate extract 1 to obtain concentrate 1;

[0065] The filter residue is soaked in ethanol (the soaking temperature is 45° C. and the soaking time is 1 hour), the weight ratio of the filter residue to the ethanol is 1:8, and ultrasonic extraction is performed, wherein the ultrasonic extraction process is firstly performed at 30 KHz for 5 minutes, then at 85 KHz for 8 minutes, then at 30 KHz for 5 minutes, then at 85 KHz for 8 minutes, and then filtered to obtain extract 2, and the extract 2 is concentrated to obtain a concentrated solution 2;

[0066] The concentrated solution 1 and the concentrated solution 2 are mixed and dried to finally obtain the Scutellaria baicalensis extract.

[0067] The above concentration process is a conventional process.

[0068] A method for preparing an antibacterial composition comprises the following steps:

[0069] The components are mixed to obtain an antibacterial composition.

[0070] Example 2

[0071] An antibacterial composition comprises, by weight, 8 parts of cinnamon extract, 5 parts of aloe extract, 2 parts of notoginseng extract and 7 parts of scutellaria baicalensis extract.

[0072] The preparation method of cinnamon extract comprises the following steps:

[0073] Take cinnamon, crush it, mix it with water, heat it to boil in a distillation device, collect the product, and obtain cinnamon extract;

[0074] The weight ratio of cinnamon to water is 1:9, and the boiling time is 5 hours;

[0075] After the product was collected, it was dried over anhydrous sodium sulfate.

[0076] The preparation method of aloe extract comprises the following steps:

[0077] Take aloe vera, crush it, add ethanol to soak it, the weight ratio of aloe vera to ethanol is 1:10, the temperature of adding ethanol to soak it is 60°C, the time of adding ethanol to soak it is 2 hours, then use microwave radiation to extract it, the power of microwave radiation is 200W, the time of microwave radiation is 5 minutes, then filter to obtain extract a and filter residue, and recover the solvent to obtain concentrated solution a;

[0078] The filter residue is soaked in a water-saturated n-butanol solution, the weight ratio of the filter residue to the water-saturated n-butanol solution is 1:8, and ultrasonic extraction is used. The ultrasonic extraction process is firstly to extract at 50KHz for 20 minutes, filter to obtain an extract b, and recover the solvent to obtain a concentrated solution b;

[0079] The concentrated solution a and the concentrated solution b are mixed and dried to finally obtain the aloe vera extract.

[0080] The preparation method of Panax notoginseng extract comprises the following steps:

[0081] Take Panax notoginseng, crush it, and then mix it with water, the weight ratio of Panax notoginseng to water is 1:5, heat it to boiling in a distillation device, the boiling time is 5 hours, collect the product, and dry it with anhydrous sodium sulfate to obtain Panax notoginseng extract.

[0082] The preparation method of the scutellaria baicalensis extract comprises the following steps:

[0083] Take scutellaria baicalensis, crush it, soak it in water (the soaking temperature is 70°C, and the soaking time is 1.5 hours), the weight ratio of scutellaria baicalensis to water is 1:12, then use microwave radiation extraction, the microwave radiation power is 20W, the microwave radiation time is 4 minutes, then filter to obtain extract 1 and filter residue, concentrate extract 1 to obtain concentrate 1;

[0084] The filter residue is soaked in ethanol (the soaking temperature is 45° C. and the soaking time is 1 hour), the weight ratio of the filter residue to the ethanol is 1:8, and ultrasonic extraction is performed, wherein the ultrasonic extraction process is firstly performed at 30 KHz for 5 minutes, then at 85 KHz for 8 minutes, then at 30 KHz for 5 minutes, then at 85 KHz for 8 minutes, and then filtered to obtain extract 2, and the extract 2 is concentrated to obtain a concentrated solution 2;

[0085] The concentrated solution 1 and the concentrated solution 2 are mixed and dried to finally obtain the Scutellaria baicalensis extract.

[0086] The above concentration process is a conventional process.

[0087] A method for preparing an antibacterial composition comprises the following steps:

[0088] The components are mixed to obtain an antibacterial composition.

[0089] That is, the difference between Example 2 and Example 1 is that the ultrasonic extraction conditions in the preparation process of the aloe extract are changed.

[0090] Example 3

[0091] Compared with Example 1, the difference of Example 3 is that in the preparation process of the scutellaria baicalensis extract, the ultrasonic extraction is performed at 85KHz for 26 minutes, and the other components and preparation process are the same as those of Example 1. That is, Example 3 changes the conditions of the ultrasonic extraction in the preparation process of the scutellaria baicalensis extract.

[0092] Example 4

[0093] Compared with Example 1, the only difference of Example 4 is that the antibacterial composition further comprises 0.5 parts by weight of Phellodendron chinense extract. The Phellodendron chinense extract is obtained by conventional water extraction method.

[0094] Comparative Example 1

[0095] Compared with Example 1, the difference of Comparative Example 1 is that an equal amount of clove extract (the clove extract is prepared by a conventional water extraction method) is used to replace the aloe extract in Example 1, and other components and processes are the same as those in Example 1.

[0096] Comparative Example 2

[0097] Compared with Example 1, the difference of Comparative Example 2 is that the scutellaria baicalensis extract in Example 1 is replaced by an equal amount of aloe extract, and the other components and processes are the same as those in Example 1.

[0098] Comparative Example 3

[0099] Compared with Example 1, the difference of Comparative Example 3 is that the aloe extract in Example 1 is replaced by an equal amount of cinnamon extract, and the other components and processes are the same as those in Example 1.

[0100] Comparative Example 4

[0101] Compared with Example 1, the difference of Comparative Example 4 is that the Panax notoginseng extract in Example 1 is replaced with an equal amount of cinnamon extract, and the other components and processes are the same as those in Example 1.

[0102] Product effect testing

[0103] The antibacterial effect of the antibacterial compositions prepared in the above examples and comparative examples was tested.

[0104] Take a commercially available liquid LB medium, sterilize it, take the revived Staphylococcus aureus and Escherichia coli and inoculate them into 5 mL of liquid LB medium respectively, shake and culture them overnight at 37°C, and test the absorbance (OD) value of the obtained bacterial culture solution at 562 nm with a microplate reader, dilute the bacterial culture solution with a commercially available liquid LB medium to a bacterial culture solution with an absorbance (OD) value of 0.5 (that is, obtain a bacterial culture solution containing Staphylococcus aureus with an OD value of 0.5 and a bacterial culture solution containing Escherichia coli with an OD value of 0.5), and set them aside.

[0105] The antibacterial compositions prepared in the above examples and comparative examples were placed on a sterile 96-well plate, and the concentrations of the antibacterial compositions diluted with commercially available liquid LB medium were 80 μg / mL, 40 μg / mL, 20 μg / mL, 10 μg / mL, 5 μg / mL, 2.5 μg / mL, and 1.25 μg / mL, respectively. 100 μL of a bacterial culture solution containing Staphylococcus aureus with an OD value of 0.5 or a bacterial culture solution containing Escherichia coli with an OD value of 0.5 was added to each well with a pipette; the negative control well was the same volume of commercially available liquid LB medium, and the positive control well was the same volume of a bacterial culture solution containing Staphylococcus aureus with an OD value of 0.5 or a bacterial culture solution containing Escherichia coli with an OD value of 0.5. The 96-well plate was placed in a constant temperature incubator at 37°C for 24 hours and then taken out for observation. The concentration of the antibacterial composition corresponding to the wells where the test bacteria did not grow was taken as the minimum inhibitory concentration. Three groups of parallel controls were set during the experiment, and the experimental results were averaged. The results are shown in Table 1.

[0106] Table 1

[0107]

[0108]

[0109] It can be seen from Table 1 that the antibacterial effects of the antibacterial compositions prepared in Examples 1-4 of the present invention are significantly better than those in Comparative Examples 1-4.

[0110] It can also be seen from Example 1 and Comparative Examples 1-4 that the antibacterial effect of the cinnamon extract, aloe extract, Panax notoginseng extract and Scutellaria baicalensis extract used in combination with each other is significantly better than the antibacterial effect of the three extracts thereof, and the aloe extract of the present invention cannot be replaced by the clove extract.

[0111] It can be further seen from Examples 1, 2 and 3 that the preparation methods of the aloe extract and the scutellaria baicalensis extract can also have a significant impact on the antibacterial effect of the antibacterial composition.

[0112] It can also be seen from Examples 1 and 4 that further adding the Phellodendron chinense extract can improve the antibacterial effect of the antibacterial composition against Staphylococcus aureus and Escherichia coli.

[0113] The above results show that the cinnamon extract, aloe extract, Panax notoginseng extract and Scutellaria baicalensis extract of the present invention have a synergistic effect.

Claims

1. An antibacterial composition, characterized in that Including cinnamon extract, aloe extract, Panax notoginseng extract, and Scutellaria baicalensis extract.

2. The antibacterial composition according to claim 1, characterized in that By weight, the invention comprises 5-20 parts of cinnamon extract, 3-15 parts of aloe extract, 1-5 parts of notoginseng extract and 5-15 parts of scutellaria baicalensis extract.

3. The antibacterial composition according to claim 1, characterized in that The preparation method of the cinnamon extract comprises the following steps: Take cinnamon, crush it, mix it with water, heat it to boil in a distillation device, collect the product, and obtain cinnamon extract.

4. The antibacterial composition according to claim 1, characterized in that The preparation method of the aloe extract comprises the following steps: Take aloe vera, crush it, add ethanol to soak it, then use microwave radiation to extract it, then filter it to obtain an extract a and a filter residue, and recover the solvent to obtain a concentrated solution a; The filter residue is soaked in a water-saturated n-butanol solution, extracted by ultrasonication, filtered to obtain an extract solution b, and the solvent is recovered to obtain a concentrated solution b; The concentrated solution a and the concentrated solution b are mixed and dried to finally obtain the aloe vera extract.

5. The antibacterial composition according to claim 4, characterized in that The weight ratio of aloe vera to ethanol is 1:(5-15); and / or the temperature of the ethanol is 40-60°C; and / or the temperature of the ethanol soaking is 40-60°C, and the soaking time is 2-5 hours; and / or, during the microwave radiation extraction, the power of the microwave radiation is 150-200W, and the microwave radiation time is 3-8 minutes; and / or, the weight ratio of the filter residue to the water-saturated n-butanol solution is 1:(3-12); and / or, the soaking temperature of the filter residue in the water-saturated n-butanol solution is 35-60°C, and the soaking time is 0.5-1 hour; and / or, the ultrasonic extraction process is firstly extracted at 40-60KHz for 1-3 minutes, then extracted at 80-90KHz for 5-10 minutes, then extracted at 40-60KHz for 4-6 minutes, and then extracted at 80-90KHz for 5-10 minutes.

6. The antibacterial composition according to claim 1, characterized in that The preparation method of the scutellaria baicalensis extract comprises the following steps: Take Scutellaria baicalensis, crush it, soak it in water, and then extract it by microwave radiation, the power of microwave radiation is 180-200W, the time of microwave radiation is 3-8 minutes, then filter to obtain extract 1 and filter residue, concentrate extract 1 to obtain concentrated solution 1; The filter residue is soaked in ethanol and ultrasonically extracted, wherein the ultrasonic extraction process is firstly to extract at 20-30KHz for 5-10 minutes, then to extract at 85-95KHz for 5-10 minutes, then to extract at 20-30KHz for 5-10 minutes, then to extract at 85-95KHz for 5-10 minutes, and then to filter to obtain extract 2, and to concentrate extract 2 to obtain concentrate 2; The concentrated solution 1 and the concentrated solution 2 are mixed and dried to finally obtain the Scutellaria baicalensis extract.

7. The antibacterial composition according to claim 6, characterized in that The weight ratio of the scutellaria baicalensis to water is 1:(3-18); and / or the temperature of the water is 70-80°C; and / or the weight ratio of the filter residue to ethanol is 1:(6-15); and / or the immersion temperature of the filter residue in ethanol is 35-60°C, and the immersion time is 0.5-1.5 hours.

8. The antibacterial composition according to any one of claims 1 to 7, characterized in that: The antibacterial composition also includes a Phellodendron chinense extract.

9. The method for preparing the antibacterial composition according to any one of claims 1 to 8, characterized in that: The following steps are involved: The components are mixed to obtain the antibacterial composition.

10. Use of the antibacterial composition according to any one of claims 1 to 8 in the preparation of antibacterial products.

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