Traditional Chinese medicine composition for preventing and treating lung heat cough and asthma of livestock as well as preparation method and application of traditional Chinese medicine composition

Abstract

The invention belongs to the technical field of traditional Chinese veterinary medicines, and relates to a traditional Chinese medicine composition for preventing and treating lung heat cough and asthma of livestock and a preparation method and application thereof, and the traditional Chinese medicine composition is prepared from the following raw materials: rhizoma atractylodis, astragalus membranaceus, white mulberry root-bark, rhizoma anemarrhenae, semen lepidii, semen armeniacae amarae, amomum tsao-ko and the like. The traditional Chinese medicine composition not only can clear away heat and toxic materials, clear away lung heat and relieve cough and asthma, but also has the effects of nourishing blood and preventing miscarriage and the like, and can quickly relieve lung heat cough and asthma to quickly stop abortion. According to the traditional Chinese medicine composition, part of the traditional Chinese medicine raw materials are pretreated through a steam explosion technology, compared with traditional decoction, extracted medicinal components are more comprehensive, the clinical medicinal effect is more reliable, and the traditional Chinese medicine composition is suitable for being used by livestock with lung heat, cough and asthma and abortion.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese veterinary medicines, and particularly relates to a traditional Chinese medicine composition for preventing and treating lung-heat cough and asthma in livestock, and a preparation method and application thereof. Background Art

[0002] In the pig farming industry, pig lung heat cough and asthma are common respiratory diseases, which are mostly caused by exogenous wind-heat, pathogenic heat blocking the lungs, improper feeding and management, poor ventilation in pens, etc. Sick pigs show symptoms such as fever, cough, asthma, and dyspnea, which not only affect the growth and development of pigs, but can also lead to death in severe cases, causing huge economic losses. From the perspective of Western medicine, blue ear virus, mycoplasma pneumonia, and contagious pleuropneumonia are mostly manifested as lung heat cough and asthma. At the same time, sow abortion is also a thorny problem in the pig farming process. Its causes are complex. In addition to infection with certain viruses and bacteria (such as blue ear disease virus, Brucella, etc.), excessive heat and imbalance of qi and blood in the sow's body can also induce abortion, which brings a heavy blow to farmers.

[0003] Table 1 Syndrome types and clinical manifestations of lung-heat cough and asthma

[0004] .

[0005] At present, the prevention and treatment of pig lung heat cough and abortion mostly rely on chemical drugs, but the long-term use of chemical drugs is prone to drug resistance and drug residues, threatening food safety and the ecological environment. Traditional Chinese veterinary medicines, with their advantages of being natural, having few toxic side effects, and not prone to drug resistance, provide a new direction for the prevention and treatment of pig diseases. However, some existing Chinese veterinary medicine prescriptions have defects such as uncertain efficacy, complex formulations, or inconvenient use in the treatment of pig lung heat cough and abortion, which makes it difficult to meet the actual needs of the pig farming industry.

[0006] Traditional Chinese medicine believes that pig lung heat cough and asthma are mostly caused by exogenous wind heat or wind cold entering the body and transforming into heat, which accumulates in the lungs and causes the lungs to fail to descend. Miscarriage is related to factors such as insufficient qi and blood, spleen and kidney deficiency, qi stagnation and blood stasis. The present invention is based on the core treatment principle of veterinary medicine for lung heat cough and asthma, "clearing heat and detoxifying, promoting lung and relieving asthma, strengthening the body and eliminating evil", and treats the symptoms in an emergency (acute phase), and reuses medicinal materials that clear lung heat (antiviral + inhibiting inflammatory storm); treats the root cause in a slow manner (recovery phase), focusing on replenishing qi and yin (immune repair).

[0007] Chinese patent CN 116077578 A discloses a Chinese medicine composition for treating blue ear disease in pigs and its application, aiming to develop a Chinese medicine preparation that can simultaneously treat blue ear disease and improve symptoms such as fever, cough, asthma, skin erythema, etc. caused by blue ear disease, and can also improve the growth performance of sick pigs, but does not disclose the beneficial effects on nursery pigs and sows' fetal preservation.

[0008] Chinese patent CN103041286A discloses a Chinese medicine composition with antitussive and antiasthmatic functions and a preparation method thereof. The composition contains ingredients such as Anemarrhena asphodeloides, Fructus Aurantii Immaturus, Ephedra sinica, Platycodon grandiflorum, Apricot kernel, Lepidium tiliaceus, Morus alba bark, Citrus reticulatae, Gypsum, Peucedanum peucedanum, Belamcanda chinensis, Eriobotrya japonicae, Ligusticum chuanxiong, Licorice, Scutellaria baicalensis, Honeysuckle, Curcuma aromatica, and Forsythia suspensa. The composition is only used to treat lung-heat cough and asthma in livestock, and does not disclose improvement of other symptoms caused by lung-heat cough and asthma, such as miscarriage.

[0009] Chinese patent CN107050167A discloses a traditional Chinese medicine composition for preventing and treating lung heat cough and asthma in pigs, and its preparation method and preparation. The composition consists of ephedra, scutellaria, apricot, gypsum, scutellaria baicalensis, mulberry bark, perilla seed, houttuynia cordata and licorice. Its therapeutic indications are only common pneumonia, lung heat cough and asthma and other diseases, and do not involve the prevention and control of viral diseases.

[0010] In summary, technical personnel in this field urgently need to develop a Chinese medicine composition that can simultaneously treat lung heat, cough and asthma, and can improve symptoms such as miscarriage caused by lung heat, cough and asthma, and can clear away heat without damaging the body, strengthen the body without aiding evil, and can be used periodically safely to achieve both prevention and treatment. Summary of the invention

[0011] After in-depth and meticulous experiments, the present invention has successfully developed a Chinese veterinary medicine composition for preventing and treating lung heat, cough and asthma in livestock. The composition can not only clear away heat and moisten the lungs, relieve cough and asthma, and effectively prevent and treat pig lung heat, cough and asthma, but also has the effects of nourishing blood and calming the fetus, can reduce the risk of sow abortion, and is safe and residue-free. The preparation method of the Chinese veterinary medicine composition of the present invention can improve the extraction rate of active ingredients and increase the drug loading of traditional decoctions through process technology upgrades, thereby improving the therapeutic effect of the disease.

[0012] The technical solution of the present invention is as follows:

[0013] In a first aspect, the present invention provides a traditional Chinese medicine composition for preventing and treating lung heat, cough and asthma in livestock, wherein the traditional Chinese medicine composition is composed of the following raw materials: Atractylodes lancea, Anemarrhena asphodeloides, Licorice, Poria cocos, Astragalus membranaceus, Houttuynia cordata, Morus alba, Lemongrass seeds, Apricot kernel, Amomum villosum, Gypsum ​​Plantago asiatica, Patchouli, Artemisia annua, Perilla leaf, Perilla stem, Lycium bark, and Atractylodes macrocephala.

[0014] The weight proportions of the raw materials in the traditional Chinese medicine composition are as follows: 1-12 parts of Atractylodes lancea, 1-10 parts of Anemarrhena asphodeloides, 1-10 parts of Licorice, 2-15 parts of Poria cocos, 1-12 parts of Astragalus, 2-15 parts of Houttuynia cordata, 2-15 parts of Morus alba bark, 2-15 parts of Lepidium officinale seeds, 2-15 parts of Apricot kernel, 2-15 parts of Amomum villosum, 2-15 parts of Gypsum Fibrosum, 2-15 parts of Pinellia ternata, 2-15 parts of Patchouli, 1-10 parts of Artemisia annua, 1-10 parts of Perilla leaf, 1-10 parts of Perilla stem, 1-10 parts of Lycium bark, and 1-10 parts of Atractylodes macrocephala.

[0015] Preferably, the weight proportions of each raw material in the traditional Chinese medicine composition are: 5-10 parts of Atractylodes lancea, 1-5 parts of Anemarrhena asphodeloides, 1-5 parts of Licorice, 5-10 parts of Poria, 2-7 parts of Astragalus, 3-7 parts of Houttuynia cordata, 4-10 parts of Morus alba, 2-7 parts of Lemongrass seeds, 2-7 parts of Apricot kernel, 3-7 parts of Amomum villosum, 4-10 parts of Gypsum, 2-7 parts of Pinellia ternata, 3-7 parts of Patchouli, 2-6 parts of Artemisia annua, 1-5 parts of Perilla leaves, 1-5 parts of Perilla stems, 1-5 parts of Lycium bark, and 1-5 parts of Atractylodes macrocephala.

[0016] More preferably, the weight proportions of each raw material in the traditional Chinese medicine composition are: 9 parts of Atractylodes lancea, 3.6 parts of Anemarrhena asphodeloides, 3.6 parts of Licorice, 9 parts of Poria, 5.4 parts of Astragalus, 6 parts of Houttuynia cordata, 8.2 parts of Morus alba, 5.2 parts of Lemongrass seeds, 5.4 parts of Apricot kernel, 6 parts of Amomum villosum, 8.2 parts of Gypsum, 5.4 parts of Pinellia ternata, 6 parts of Patchouli, 4.6 parts of Artemisia annua, 3.6 parts of Perilla leaves, 3.6 parts of Perilla stems, 3.6 parts of Lycium bark, and 3.6 parts of Atractylodes macrocephala.

[0017] The Chinese medicine composition for preventing and treating lung heat, cough and asthma in livestock is weighed according to weight, crushed and sieved respectively, mixed evenly, and prepared into Chinese medicine powder for animal use.

[0018] In a second aspect, the present invention provides a method for preparing a Chinese medicine composition for preventing and treating lung heat, cough and asthma in livestock, the preparation steps comprising: (1) weighing each raw material component by weight, and pre-treating the five Chinese medicines in the Chinese medicine composition, namely, Atractylodes lancea, Atractylodes macrocephala, Morus alba bark, Amomum villosum, and Perilla frutescens stem, by steam explosion to obtain steam explosion raw materials; (2) placing the obtained steam explosion raw materials and the remaining 13 Chinese medicine raw materials in the same extraction tank for water extraction and concentration, and spray drying to obtain dry powder; and (3) preparing various Chinese medicine preparations according to conventional processes for veterinary drug preparations.

[0019] The conditions of the steam explosion pretreatment in step (1) are: pressure 1-12 MPa, pressure holding time 10s-15 minutes, and temperature controlled at 100-250°C. This process can effectively destroy the cell wall structure of traditional Chinese medicine, making the effective ingredients in the cells easier to dissolve. Compared with traditional processing methods, it can greatly improve the extraction efficiency, thereby enhancing the clinical treatment effect.

[0020] The water extraction and concentration step in step (2) is as follows: weigh Rhizoma Anemarrhenae, Radix Glycyrrhizae, Poria, Radix Astragali, Herba Houttuyniae, Semen Lepidii, Apricot Kernel, Gypsum Fibrosum, Rhizoma Pinelliae, Herba Patchouli, Artemisia Annua, Folium Perillae, and Cortex Lycii by weight, place them in the same extraction tank with the steam-exploded raw materials, extract them with 6 to 10 times the amount of water (equivalent to the weight of the medicinal materials) by reflux for 1 to 3 times, each time for 0.5 to 2 hours, combine the extracts, and concentrate under reduced pressure to a thick paste with a relative density of 1.10 to 1.20 (55° C.).

[0021] The unique steam explosion pretreatment process combined with the subsequent reasonable preparation process can extract the effective ingredients of traditional Chinese medicine to the greatest extent, improve the bioavailability of the drugs, reduce the dosage of the drugs, reduce the breeding costs, and avoid chemical residues, ensuring the quality and safety of pork, which is in line with the development trend of green breeding.

[0022] The third aspect of the present invention provides the use of the traditional Chinese medicine composition for preventing and treating lung heat, cough and asthma in livestock in medicine for preventing and treating lung heat, cough and asthma in pigs and reducing the risk of abortion in sows.

[0023] The fourth aspect of the present invention provides the use of the traditional Chinese medicine composition for preventing and treating lung heat, cough and asthma in the preparation of drugs for preventing and treating porcine blue ear disease, mycoplasma pneumonia and contagious pleuropneumonia.

[0024] The Chinese veterinary medicine composition of the present invention is scientifically formulated to target the pathogenesis of pig lung heat, cough and asthma and abortion, and has a multi-target synergistic effect. It comprehensively regulates pig body functions from multiple aspects such as clearing heat, moistening the lungs, strengthening the spleen, and calming the fetus, and its preventive and therapeutic effects are significantly better than traditional prescriptions.

[0025] Atractylodes lancea dries dampness, strengthens the spleen and relieves depression, while Poria cocos promotes diuresis, eliminates dampness and calms the mind. The two are used together to target the core pathogenesis of blue ear disease "dampness and toxins blocking the lungs and spleen dysfunction", and the principle of "treating dampness first" is in line with the treatment principle of "when the dampness is gone, the heat is isolated" in "Treatise on Epidemics"; Houttuynia cordata clears heat and detoxifies; Morus alba bark purges the lungs and relieves asthma. Together they form the monarch medicine, and have a rapid therapeutic effect on the pathological stage when the pathogenic factors have entered the qi and heat and toxins block the lungs.

[0026] The seeds of Trichosanthes can purge the lungs and relieve asthma, promote diuresis and reduce swelling, while the bitter almond can relieve cough and asthma, and moisten the intestines and promote bowel movements. Together, they can purge the lungs and relieve asthma, descend qi and promote diuresis, and help the main medicine relieve lung qi stagnation, forming a "promoting upward and unblocking downward" trend, which is in line with the "lung and large intestine are exterior and interior to each other" theory; the seeds of Anemarrhena can clear away heat and purge fire, nourish yin and moisten dryness, while the seeds of Gypsum can clear away heat and purge fire, and relieve restlessness and quench thirst. Both of them clear qi and resolve real heat, and block the heat evil from damaging body fluids. They are the core drug pair variations of Baihu Decoction, targeting high fever and retention, and are all assistant drugs.

[0027] Amomum villosum can dry dampness and warm the middle, stop malaria and eliminate phlegm, while Pinellia ternata can dry dampness and resolve phlegm, reduce adverse effects and stop vomiting. Both of them can clear turbidity with aromatic properties, dry dampness and resolve phlegm, cut off the formation of dampness and turbidity, embody the idea of ​​"treating disease before it occurs", and prevent dampness and turbidity from obscuring the pericardium; Astragalus + Atractylodes macrocephala can replenish Qi and strengthen the exterior, strengthen the body and eliminate evil, prevent excessive attack, and comply with the principle of "eliminating evil without hurting the body"; Artemisia annua + Rehmannia root bark can clear away virtual heat and prevent heat evil from deeply lurking in the yin, and are used as adjuvants for the common low-grade fever in the late stage of blue ear disease.

[0028] Perilla leaves and stems can invigorate the lungs and lead the medicine upward, and they also have the effect of dispelling exterior pathogens and reducing fever; licorice harmonizes the various medicines, alleviates the severe cold nature, nourishes the spleen and replenishes qi, and assists in transportation and transformation, and they are all guiding medicines.

[0029] The whole formula has the effects of clearing away heat and moistening the lungs, relieving cough and asthma, strengthening the spleen and drying dampness, and calming the fetus. It is effective in preventing and treating pig lung heat, cough, asthma and miscarriage.

[0030] The Chinese medicine composition complies with the "separate elimination and dissipation" treatment principle of "Treatise on Febrile Diseases", has a balanced system of clearing away heat without promoting dampness, and removing dampness without promoting heat, and multi-target intervention of Chinese veterinary medicine, embodies the idea of ​​"intercepting and reversing" the transmission of epidemic diseases, and is of great value for preventing and controlling the viremia period.

[0031] The beneficial effects of the present invention are:

[0032] 1. The present invention is based on the theory of traditional Chinese medicine, aims at the etiology and pathogenesis of pig lung heat cough and asthma, and abortion, scientifically formulates the prescription, and coordinates the drugs to treat both the symptoms and the root causes.

[0033] 2. The raw materials of the Chinese veterinary medicine composition of the present invention are all natural Chinese medicinal materials, which are green and environmentally friendly, have little toxic and side effects, are not easy to produce drug residues in pigs, are safe to eat, and meet the development needs of modern animal husbandry for green breeding and antibiotic-free breeding.

[0034] 3. The preparation process is unique, using methods such as steam explosion, water extraction, and spray drying. It is easy to extract the full-effect ingredients and control the operation. It has low cost and is suitable for large-scale industrial production, and is easy to promote and apply in the pig farming industry. DETAILED DESCRIPTION

[0035] The above contents will be further described in detail below through specific implementation methods in the form of embodiments. However, this should not be understood as the scope of the above subject matter being limited to the following embodiments. All technologies realized based on the contents of the present invention are within the scope.

[0036] Example 1 Preparation of soluble powder

[0037] Formula: 90kg of Atractylodes lancea, 36kg of Anemarrhena asphodeloides, 36kg of Licorice, 90kg of Poria cocos, 54kg of Astragalus, 60kg of Houttuynia cordata, 82kg of Morus alba, 52kg of Lemongrass, 54kg of Apricot kernel, 60kg of Amomum villosum, 82kg of Gypsum, 54kg of Pinellia ternata, 60kg of Patchouli, 46kg of Artemisia annua, 36kg of Perilla leaf, 36kg of Perilla stem, 36kg of Lycium bark, and 36kg of Atractylodes macrocephala. Preparation method: Accurately weigh each raw material according to the above weight portions, wherein Atractylodes lancea, Atractylodes macrocephala, Morus alba, Amomum villosum, and Perilla stem are treated by steam explosion process, with steam explosion pressure of 1.5MPa and maintenance time of 60s. After the steam explosion, add the remaining medicinal materials, add 10 times the amount of water, decoct and extract twice, each time for 1.5 hours, combine the extracts, concentrate under reduced pressure to a relative density of 1.2, spray dry (inlet air temperature 160℃, outlet air temperature 90℃), and make into a soluble powder.

[0038] Example 2 Preparation of powder

[0039] Formula: 90kg of Atractylodes lancea, 36kg of Anemarrhena, 36kg of Licorice, 90kg of Poria, 54kg of Astragalus, 60kg of Houttuynia, 82kg of Morus alba, 52kg of Lemongrass, 54kg of Apricot, 60kg of Amomum villosum, 82kg of Gypsum, 54kg of Pinellia ternata, 60kg of Patchouli, 46kg of Artemisia annua, 36kg of Perilla leaf, 36kg of Perilla stem, 36kg of Lycium bark, and 36kg of Atractylodes macrocephala. Preparation method: Weigh the above medicinal materials according to the prescription amount, crush them separately, pass through a 24-mesh sieve, and mix them evenly.

[0040] Example 3 Preparation of oral solution.

[0041] Formula: 80kg of Atractylodes lancea, 40kg of Anemarrhena asphodeloides, 40kg of Licorice, 80kg of Poria cocos, 50kg of Astragalus, 60kg of Houttuynia cordata, 80kg of Morus alba, 50kg of Lemongrass, 50kg of Apricot kernel, 60kg of Amomum villosum, 80kg of Gypsum, 50kg of Pinellia ternata, 60kg of Patchouli, 55kg of Artemisia annua, 30kg of Perilla leaf, 40kg of Perilla stem, 40kg of Lycium bark, and 40kg of Atractylodes macrocephala. Preparation method: Accurately weigh each raw material according to the above weight portions, wherein Atractylodes lancea, Atractylodes macrocephala, Morus alba, Amomum villosum, and Perilla stem are treated by steam explosion process, with steam explosion pressure of 2.5MPa and maintenance time of 80s. After the steam explosion, add the remaining medicinal materials and 10 times the amount of water, decoct and extract twice, each time for 2 hours, combine the extracts, concentrate under reduced pressure to a relative density of 1.2, filter with a 300-mesh filter cloth, and prepare a concentration of 1.5 kg of original medicinal materials per kg of liquid medicine. Mix evenly, sterilize with circulating steam for 30 minutes, and fill.

[0042] Example 4 Preparation of granules

[0043] Formula: 60kg of Atractylodes lancea, 30kg of Anemarrhena asphodeloides, 30kg of Licorice, 90kg of Poria cocos, 50kg of Astragalus, 40kg of Houttuynia cordata, 60kg of Morus alba, 40kg of Lemongrass, 40kg of Apricot kernel, 60kg of Amomum villosum, 80kg of Gypsum, 40kg of Pinellia ternata, 50kg of Patchouli, 30kg of Artemisia annua, 30kg of Perilla leaf, 30kg of Perilla stem, 30kg of Lycium bark, and 30kg of Atractylodes macrocephala. Preparation method: Accurately weigh each raw material according to the above weight parts, wherein Atractylodes lancea, Atractylodes macrocephala, Morus alba, Amomum villosum, and Perilla stem are treated by steam explosion process, with steam explosion pressure of 2.0MPa and maintenance time of 120s. After the steam explosion, add the remaining medicinal materials, add 10 times the amount of water, decoct and extract twice, each time for 1.5 hours, combine the extracts, concentrate under reduced pressure to a relative density of 1.2, filter with 300 mesh filter cloth, mix evenly with 50kg of dextrin and 60kg of powdered sugar, use 60% ethanol as a binder, make into granules, dry, granulate, and package.

[0044] Example 5 Preparation of soluble powder

[0045] Formula: 50kg of Atractylodes lancea, 10kg of Anemarrhena asphodeloides, 10kg of Licorice, 50kg of Poria, 20kg of Astragalus, 30kg of Houttuynia cordata, 40kg of Morus alba, 20kg of Lemongrass, 20kg of Apricot kernel, 30kg of Amomum villosum, 40kg of Gypsum, 20kg of Pinellia ternata, 30kg of Patchouli, 20kg of Artemisia annua, 10kg of Perilla leaf, 10kg of Perilla stem, 10kg of Lycium bark, and 10kg of Atractylodes macrocephala. Preparation method: Accurately weigh each raw material according to the above weight portions, wherein Atractylodes lancea, Atractylodes macrocephala, Morus alba, Amomum villosum, and Perilla stem are treated by steam explosion process, with steam explosion pressure of 3.5MPa and maintenance time of 60s. After the steam explosion, add the remaining medicinal materials, add 10 times the amount of water, decoct and extract twice, each time for 1.5 hours, combine the extracts, concentrate under reduced pressure to a relative density of 1.2, spray dry (inlet air temperature 160℃, outlet air temperature 90℃), and make into a soluble powder.

[0046] Example 6 Preparation of soluble powder

[0047] Formula: 100kg of Atractylodes, 50kg of Anemarrhena, 50kg of Licorice, 100kg of Poria, 70kg of Astragalus, 70kg of Houttuynia, 100kg of Morus alba, 70kg of Lemongrass, 70kg of Apricot, 70kg of Amomum villosum, 100kg of Gypsum, 70kg of Pinellia ternata, 70kg of Patchouli, 60kg of Artemisia annua, 50kg of Perilla leaf, 50kg of Perilla stem, 50kg of Lycium bark, and 50kg of Atractylodes macrocephala. Preparation method: Accurately weigh each raw material according to the above weight parts, wherein Atractylodes, Atractylodes, Morus alba, Amomum villosum, and Perilla stem are treated by steam explosion process, with steam explosion pressure of 3.5MPa and maintenance time of 60s. After the steam explosion, add the remaining medicinal materials, add 10 times the amount of water, decoct and extract twice, each time for 1.5 hours, combine the extracts, concentrate under reduced pressure to a relative density of 1.2, spray dry (inlet air temperature 160℃, outlet air temperature 90℃), and make into a soluble powder.

[0048] Comparative Example 1 Preparation of soluble powder

[0049] The formula is consistent with that in Example 1, and the preparation process is as follows: Atractylodes lancea, Rhizoma Anemarrhenae, Glycyrrhiza uralensis, Poria cocos, Astragalus membranaceus, Houttuynia cordata, Morus alba, Semen Lepidium, Apricot kernel, Amomum villosum, Gypsum foetida, Pinellia ternata, Agastache rugosa, Artemisia annua, Perilla leaf, Perilla stem, Cortex Lycii, and Atractylodes macrocephala are all treated by steam explosion and then water extracted, wherein the steam explosion and water extraction process conditions are the same as those in Example 1.

[0050] Comparative Example 2

[0051] The Chinese medicine preparation was prepared by adopting the formula and process in Example 3 of patent CN116077578A.

[0052] Example 7 Clinical effect test on prevention and treatment of blue ear disease

[0053] 1. Experimental drugs

[0054] 30 kg each of the products of Example 1, Examples 5-6, and Comparative Examples 1-2 of the present invention, products of the control group, a marker pen, and blood collection consumables (syringe, vacuum blood collection tube, 2.0 ml EP tube, etc.).

[0055] 2. Experimental Methods

[0056] 2.1 Grouping of experimental pigs

[0057] A 5,000-sow farm in Fuzhou, Fujian, has always been blue ear positive, and weaned pigs generally have lung heat, cough and asthma. On July 21, 2024, 298 weaned piglets were transferred from the delivery room 10 to the nursery 1-1 house; they were divided into 6 groups according to the feed line, east-west, middle, north-south, as follows.

[0058] Table 2 Grouping and medication

[0059] .

[0060] 2.2 Collecting samples after grouping

[0061] Before and after the test, 10 blood samples were randomly collected from each of the product groups of Example 1, Example 5, Example 6, Comparative Example 1, and Comparative Example 2, and 10 blood samples were randomly collected from each of the product groups of Example 1, Example 5, Example 6, Comparative Example 1, and Comparative Example 2, for a total of 60 blood samples; the ear tags were marked with 01-10, 11-20, 21-30, 31-40, 41-50, and 51-60, respectively; the ear tag of the control group was marked with 51-60. At the same time, the detection of the S / P value of blue ear antibodies and the detection of PRRSV pathogens were carried out to evaluate the current PRRSV infection level of the test pig farm and ensure that it was at the same infection level.

[0062] 3. Therapeutic medication

[0063] The product group of Example 1, the product group of Example 5, the product group of Example 6, the product group of Comparative Example 1, and the product group of Comparative Example 2 were treated with medication at the same time, with each group of products at 3 kg / ton, and the medication was used for 14 consecutive days.

[0064] The 48 heads of the control group were given 20% tilmicosin premix 2kg / ton + 10% compound amoxicillin 1kg / / ton in feed for 14 consecutive days.

[0065] 4. Experimental Results

[0066] Collection of production data and evaluation of experimental data in nursery-growing pig therapeutic trials.

[0067] 1. S / P value of blue ear antibody and PRRSV pathogen value before and after the test.

[0068] The S / P values ​​of blue ear antibodies and PRRSV pathogen values ​​before and after the test in each test group and control group were statistically analyzed, and the average value and dispersion of the test report were calculated. The statistical results are shown in the following table.

[0069] Table 3 Comparative analysis of test data between the experimental group and the control group

[0070] .

[0071] Table 4 Inspection record form during the experiment

[0072] .

[0073] Note: The sick pigs include but are not limited to those with symptoms such as coughing, rapid breathing, emaciation, lameness, anorexia, depression, and stiff pigs. Videos of the pigs are taken every day, with a length of about 1 minute. The photographer describes the time, location, and status of the pigs.

[0074] The so-called pig picking refers to the process in which breeders or professional technicians conduct comprehensive and detailed observation of a pen of pigs, identify individuals with health problems or abnormal growth and development based on multiple indicators such as the pigs' appearance, behavioral performance, and mental state, select them from the original breeding group, and transfer them to a separate pen for special breeding.

[0075] The purpose of pig selection is to focus on and manage pigs with potential problems in a targeted manner so as to detect and treat diseases in a timely manner, improve their health status, avoid possible disease spread, and ensure the health and production benefits of the entire pig herd.

[0076] 5. Experimental Conclusion

[0077] From the analysis of blue ear antibody S / P value and PRRSV pathogen, compared with before and after the test, the blue ear antibody S / P value and PRRSV pathogen detected in the blood sample of the product group of Example 1 were significantly reduced; the product group of Example 5, the product group of Example 6, the product group of Comparative Example 1, and the product group of Comparative Example 2 had certain effects, but the cure rate was significantly different from that of the product of Example 1. The control group had a tendency to recover, but it was far less than the group using traditional Chinese medicine. The product group of Example 1 was slightly better than the product group of Example 5 and the product group of Example 6, but the difference was not significant; the product group of the embodiment of the present invention was significantly different from the product group of Comparative Example 1, the product group of Comparative Example 2, and the control group; the product group of Comparative Example 1 and the product group of Comparative Example 2 were not as good as the other test groups in laboratory detection indicators, but they were better than the control group.

[0078] Based on the mortality and clinical observations, it can be concluded that the Chinese veterinary drug compositions of Examples 1, 5, and 6 of the present invention are significantly better than those of the comparative example group and the control group in treating pig lung heat cough and asthma, can effectively alleviate the symptoms of pigs, and improve the cure rate, fully demonstrating the good application prospects of the Chinese veterinary drug compositions of the present invention in preventing and treating blue ear disease in pigs.

[0079] Example 8 Clinical trial on preventing and treating abortion in pregnant sows

[0080] In order to verify the clinical efficacy of the veterinary drug composition of the present invention, the following experiments were conducted:

[0081] Experimental animals: A pig farm with positive mycoplasma and blue ear tests was selected, and 240 pregnant sows showing signs of abortion were selected and randomly divided into 6 groups, with 40 sows in each group.

[0082] 1. Test drug and dosage

[0083] Experimental group 1: The Chinese veterinary drug composition prepared in Example 1 of the present invention was administered at a dosage of 3 kg / ton of feed for 14 consecutive days.

[0084] Experimental Group 2: The Chinese veterinary drug composition prepared in Example 5 of the present invention was administered at a dosage of 3 kg / ton of feed for 14 consecutive days.

[0085] Experimental group 3: The Chinese veterinary drug composition prepared in Example 6 of the present invention was administered at a dosage of 3 kg / ton of feed for 14 consecutive days.

[0086] Experimental Group 4: The Chinese veterinary medicine composition prepared in Comparative Example 1 of the present invention was administered at a dosage of 3 kg / ton of material for 14 consecutive days.

[0087] Experimental Group 5: The Chinese veterinary medicine composition prepared in Comparative Example 2 of the present invention was administered at a dosage of 3 kg / ton of material for 14 consecutive days.

[0088] Control group: 2kg of commercially available 20% tilmicosin premix + 1kg of 10% compound amoxicillin powder / ton of material were used for continuous administration for 14 days.

[0089] 2. Test methods

[0090] Observe and record changes in symptoms such as coughing, panting, body temperature, etc. of pigs, record sow miscarriage, and calculate the success rate of fetal preservation and the health status of the piglets.

[0091] 3. Criteria for determining efficacy

[0092] Cure: Symptoms such as coughing and wheezing completely disappeared, body temperature returned to normal, and eating and drinking returned to normal; the sows did not suffer from miscarriage, and gave birth normally on the expected date, and the piglets born were healthy and lively.

[0093] Effective: Symptoms such as coughing and wheezing were significantly alleviated, body temperature was basically normal, and eating and drinking were improved; sow abortion was significantly reduced, and sows gave birth normally on the expected date, and more than 90% of the piglets born were healthy and lively.

[0094] Ineffective: No significant improvement or aggravation of symptoms; miscarriage in sows exceeds 3%.

[0095] 4. Test results

[0096] The symptoms of different test groups were statistically analyzed, the differences between different groups were analyzed, and the effects were evaluated. The statistical results are shown in the table below.

[0097] Table 5 Symptom improvement and effects in different experimental groups

[0098] .

[0099] The test results show that the Chinese veterinary medicine composition of the present invention is significantly better than the control group in treating pig lung heat, cough and asthma and preventing sow abortion. It can effectively alleviate the symptoms of pigs, improve the cure rate, the success rate of fetal preservation and the health rate of piglets produced, which fully demonstrates the good application prospects of the Chinese veterinary medicine composition of the present invention in preventing and treating pig lung heat, cough and asthma and abortion.

[0100] Although the specific implementation modes of the present invention are described above, those skilled in the art should understand that the specific implementation modes described are only illustrative and are not intended to limit the scope of the present invention. The equivalent modifications and changes made by those skilled in the art in accordance with the spirit of the present invention should be included in the scope of protection of the claims of the present invention.

Claims

1. A Chinese medicine composition for preventing and treating lung-heat cough and asthma in livestock, characterized in that: The Chinese medicine composition is composed of the following raw materials: atractylodes, rhizoma anemarrhenae, liquorice, tuckahoe, astragalus, houttuynia cordata, white mulberry bark, scutellaria baicalensis, bitter almond, tsaoko, gypsum, pinellia tuber, patchouli, artemisia annua, perilla leaf, perilla stem, cortex lycii and atractylodes.

2. The Chinese medicine composition according to claim 1, characterized in that: The Chinese medicine composition is prepared by mixing the following raw materials in proportion by weight: 1-12 parts of Atractylodes lancea, 1-10 parts of Anemarrhena asphodeloides, 1-10 parts of Licorice, 2-15 parts of Poria cocos, 1-12 parts of Astragalus, 2-15 parts of Houttuynia cordata, 2-15 parts of Morus alba bark, 2-15 parts of Lepidium officinale seeds, 2-15 parts of Apricot kernel, 2-15 parts of Amomum villosum, 2-15 parts of Gypsum Fibrosum, 2-15 parts of Pinellia ternata, 2-15 parts of Patchouli, 1-10 parts of Artemisia annua, 1-10 parts of Perilla leaf, 1-10 parts of Perilla stem, 1-10 parts of Lycium bark, and 1-10 parts of Atractylodes macrocephala.

3. The Chinese medicine composition according to claim 2, characterized in that: The Chinese medicine composition is prepared by mixing the following raw materials in proportion by weight: 5-10 parts of Atractylodes lancea, 1-5 parts of Anemarrhena asphodeloides, 1-5 parts of Licorice, 5-10 parts of Poria cocos, 2-7 parts of Astragalus, 3-7 parts of Houttuynia cordata, 4-10 parts of Morus alba bark, 2-7 parts of Lepidium officinale seeds, 2-7 parts of Apricot kernel, 3-7 parts of Amomum villosum, 4-10 parts of Gypsum Fibrosum, 2-7 parts of Pinellia ternata, 3-7 parts of Patchouli, 2-6 parts of Artemisia annua, 1-5 parts of Perilla leaf, 1-5 parts of Perilla stem, 1-5 parts of Lycium bark, and 1-5 parts of Atractylodes macrocephala.

4. The Chinese medicine composition according to claim 3, characterized in that: The Chinese medicine composition is prepared by mixing the following raw materials in proportion by weight: 9 parts of Atractylodes lancea, 3.6 parts of Anemarrhena asphodeloides, 3.6 parts of Licorice, 9 parts of Poria, 5.4 parts of Astragalus, 6 parts of Houttuynia cordata, 8.2 parts of Morus alba, 5.2 parts of Lemongrass seeds, 5.4 parts of Apricot kernel, 6 parts of Amomum villosum, 8.2 parts of Gypsum, 5.4 parts of Pinellia ternata, 6 parts of Patchouli, 4.6 parts of Artemisia annua, 3.6 parts of Perilla leaf, 3.6 parts of Perilla stem, 3.6 parts of Lycium bark, and 3.6 parts of Atractylodes macrocephala.

5. The Chinese medicine composition according to any one of claims 1 to 4, characterized in that: The Chinese medicine composition is weighed according to weight, crushed and sieved respectively, and then mixed evenly to prepare a Chinese medicine powder for animal use.

6. A method for preparing a Chinese medicine composition for preventing and treating lung heat, cough and asthma in livestock as claimed in any one of claims 1 to 4, wherein the preparation steps are as follows: (1) Weighing each raw material by weight, pre-treating five Chinese medicinal materials, namely, Atractylodes lancea, Atractylodes macrocephala, Morus alba bark, Amomum villosum, and Perilla frutescens stem, by steam explosion to obtain steam-exploded raw materials; (2) placing the steam explosion raw material obtained in step (1) and other Chinese medicine raw materials in the same extraction tank for water extraction and concentration, and spray drying to obtain dry powder; (3) The dried powder in step (2) is prepared into various Chinese medicine preparations according to conventional veterinary drug preparation processes.

7. The preparation method according to claim 6, characterized in that: The conditions of the steam explosion process in step (1) are: pressure 1-12 MPa, pressure holding time 10s-15 minutes, and temperature controlled at 100-250°C.

8. The preparation method according to claim 6, characterized in that: The water extraction and concentration step in step (2) is to use water equivalent to 6 to 10 times the weight of the medicinal material for reflux extraction 1 to 3 times, each time for 0.5 to 2 hours, combine the extracts, and concentrate under reduced pressure to a thick paste with a relative density of 1.10 to 1.20 at 55°C.

9. Use of a Chinese medicine composition for preventing and treating lung heat, cough and asthma in livestock according to any one of claims 1 to 4 in the preparation of a medicine for preventing and treating lung heat, cough and asthma in pigs and reducing the risk of abortion in sows.

10. Use of a traditional Chinese medicine composition for preventing and treating lung heat, cough and asthma in livestock according to any one of claims 1 to 4 in the preparation of medicines for preventing and treating porcine blue ear disease, mycoplasma pneumonia and contagious pleuropneumonia.