A traditional Chinese medicine composition for preventing and treating pulmonary heat cough and asthma in livestock, its preparation method and application

Abstract

The present invention belongs to the technical field of traditional Chinese veterinary medicine, and relates to a traditional Chinese medicine composition for preventing and treating lung heat cough and asthma in livestock, its preparation method and application, which are prepared from raw materials such as Atractylodes lancea, Astragalus membranaceus, Mulberry Bark, Anemarrhena asphodeloides, Lepidium apetalum, Bitter Apricot Seed, Tsaoko Fruit, etc. This traditional Chinese medicine composition can not only clear heat and detoxify, clear the lung heat, relieve cough and asthma, but also has the effects of nourishing blood and preventing miscarriage, etc., and can quickly relieve lung heat cough and asthma and stop miscarriage quickly. The traditional Chinese medicine composition of the present invention adopts the steam explosion process to pretreat some traditional Chinese medicine raw materials. Compared with the traditional decoction, the extracted medicinal components are more comprehensive and the clinical medicinal effects are more reliable, and it is suitable for livestock with lung heat cough and asthma complicated with miscarriage.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese veterinary medicine, and particularly relates to a traditional Chinese medicine composition for preventing and treating pulmonary heat cough and asthma in livestock, and its preparation method and application. Background Art

[0002] In the pig breeding industry, pig pulmonary heat cough and asthma are common respiratory diseases, which are mostly caused by factors such as exogenous wind-heat, pathogenic heat congesting the lungs, improper feeding management, and poor ventilation in the pens. Diseased pigs show symptoms such as fever, cough, asthma, and dyspnea, which not only affect the growth and development of pigs, but may also lead to death in severe cases, causing huge economic losses. Judging from the perspective of Western medicine, porcine reproductive and respiratory syndrome virus, mycoplasma pneumonia, and contagious pleuropneumonia often show symptoms of pulmonary heat cough and asthma. At the same time, sow abortion is also a thorny problem in the pig breeding process. Its causes are complex. In addition to infection with certain viruses and bacteria (such as porcine reproductive and respiratory syndrome virus, Brucella, etc.), excessive heat in the sow's body and disharmony of qi and blood can also induce abortion, bringing a heavy blow to farmers.

[0003] Table 1 Syndromes and clinical manifestations related to pulmonary heat cough and asthma

[0004] 。

[0005] At present, the prevention and treatment methods for pig pulmonary heat cough and asthma mostly rely on chemical drugs. However, long-term use of chemical drugs is prone to problems such as drug resistance and drug residues, threatening food safety and the ecological environment. Traditional Chinese veterinary medicine, with its advantages of being natural, having small toxic and side effects, and being less likely to produce drug resistance, provides a new direction for the prevention and treatment of pig diseases. However, some existing traditional Chinese veterinary medicine prescriptions have defects such as inaccurate curative effects, complex formulas, or inconvenient use when treating pig pulmonary heat cough and asthma, and it is difficult to meet the actual needs of the pig breeding industry.

[0006] Traditional Chinese medicine believes that pig pulmonary heat cough and asthma are mostly caused by exogenous wind-heat or exogenous wind-cold transforming into heat, accumulating in the lungs, and the lungs losing their function of dispersing and descending. Abortion is related to factors such as qi and blood deficiency, spleen and kidney deficiency, and qi stagnation and blood stasis. According to the core treatment principles of traditional Chinese veterinary medicine for pulmonary heat cough and asthma, "clearing heat and detoxifying, dispersing the lung qi and relieving asthma, strengthening the healthy qi and eliminating pathogenic factors", treating the symptoms first in the acute stage (urgent situation), and using herbs that clear pulmonary heat (anti-virus + inhibiting inflammatory storm) in large amounts; treating the root cause in the recovery stage (slow situation), focusing on supplementing qi and yin (immune repair).

[0007] Chinese Patent CN 116077578 A discloses a traditional Chinese medicine composition for treating porcine reproductive and respiratory syndrome and its application, aiming to develop a traditional Chinese medicine preparation that can simultaneously treat porcine reproductive and respiratory syndrome, and can also improve symptoms such as fever, cough, asthma, and skin erythema caused by porcine reproductive and respiratory syndrome, and can also improve the growth performance of diseased pigs, but does not disclose the beneficial effects on raising pigs and protecting the fetuses of sows.

[0008] Chinese Patent CN103041286A discloses a traditional Chinese medicine composition with cough-relieving and asthma-calming functions and its preparation method. The components in the formula include Anemarrhena Rhizome, Fructus Aurantii, Ephedra, Platycodon Root, Semen Armeniacae Amarum, Semen Lepidii, Cortex Mori, Pericarpium Citri Reticulatae, Gypsum Fibrosum, Peucedanum Praeruptorum, Belamcanda Chinensis, Folium Eriobotryae, Licorice Root, Scutellaria Baicalensis, Flos Lonicerae, Radix Curcumae, and Fructus Forsythiae. It is only used for treating the lung heat cough and asthma in livestock, and does not disclose the improvement of other symptoms caused by lung heat cough and asthma such as miscarriage.

[0009] Chinese Patent CN107050167A discloses a traditional Chinese medicine composition for preventing and treating pig lung heat cough and asthma, its preparation method and preparation. The formula consists of Ephedra, Scutellaria Baicalensis, Apricot Kernel, Gypsum Fibrosum, Semen Lepidii, Cortex Mori, Perilla Seed, Houttuynia Cordata, and Licorice Root. The indications for its treatment are only common diseases such as pneumonia and lung heat cough and asthma, and do not involve the prevention and control of viral diseases.

[0010] In summary, those skilled in the art urgently need to develop a traditional Chinese medicine composition that can simultaneously treat lung heat cough and asthma, improve symptoms such as miscarriage caused by lung heat cough and asthma, clear heat without damaging healthy qi, strengthen healthy qi without assisting pathogenic factors, and can be safely used cyclically to achieve equal emphasis on prevention and treatment. Summary of the Invention

[0011] Through in-depth and meticulous experiments, the present invention has successfully developed a traditional Chinese veterinary medicine composition for preventing and treating lung heat cough and asthma in livestock. This composition can not only clear heat and moisten the lungs, relieve cough and calm asthma, effectively prevent and treat pig lung heat cough and asthma diseases, but also has the effect of nourishing blood and preventing miscarriage, can reduce the risk of sow miscarriage, and is safe without residues. The preparation method of the traditional Chinese veterinary medicine composition of the present invention can improve the extraction rate of active ingredients and increase the drug loading capacity of traditional decoctions through process technology upgrading, thereby improving the treatment effect of diseases.

[0012] The technical solution of the present invention is as follows:

[0013] In the first aspect of the present invention, there is provided a traditional Chinese medicine composition for preventing and treating lung heat cough and asthma in livestock. The traditional Chinese medicine composition is composed of the following raw materials: Atractylodes Rhizome, Anemarrhena Rhizome, Licorice Root, Poria, Astragalus Membranaceus, Houttuynia Cordata, Cortex Mori, Semen Lepidii, Semen Armeniacae Amarum, Tsaoko Fruit, Gypsum Fibrosum, Pinellia Rhizome Praeparata, Pogostemon Cablin, Artemisia Annua, Perilla Leaf, Perilla Stem, Cortex Lycii Radicis, and Atractylodes Macrocephala.

[0014] The weight parts of each raw material in the traditional Chinese medicine composition are: Atractylodes Rhizome 1 - 12 parts, Anemarrhena Rhizome 1 - 10 parts, Licorice Root 1 - 10 parts, Poria 2 - 15 parts, Astragalus Membranaceus 1 - 12 parts, Houttuynia Cordata 2 - 15 parts, Cortex Mori 2 - 15 parts, Semen Lepidii 2 - 15 parts, Semen Armeniacae Amarum 2 - 15 parts, Tsaoko Fruit 2 - 15 parts, Gypsum Fibrosum 2 - 15 parts, Pinellia Rhizome Praeparata 2 - 15 parts, Pogostemon Cablin 2 - 15 parts, Artemisia Annua 1 - 10 parts, Perilla Leaf 1 - 10 parts, Perilla Stem 1 - 10 parts, Cortex Lycii Radicis 1 - 10 parts, and Atractylodes Macrocephala 1 - 10 parts.

[0015] Preferably, the weight parts of each raw material in the traditional Chinese medicine composition are as follows: Atractylodes lancea 5 - 10 parts, Anemarrhena asphodeloides 1 - 5 parts, Glycyrrhiza uralensis 1 - 5 parts, Poria cocos 5 - 10 parts, Astragalus membranaceus 2 - 7 parts, Houttuynia cordata 3 - 7 parts, Morus alba root - bark 4 - 10 parts, Lepidium apetalum 2 - 7 parts, Prunus armeniaca 2 - 7 parts, Tsaoko fruit 3 - 7 parts, Gypsum fibrosum 4 - 10 parts, Pinellia ternata 2 - 7 parts, Pogostemon cablin 3 - 7 parts, Artemisia annua 2 - 6 parts, Perilla leaf 1 - 5 parts, Perilla stem 1 - 5 parts, Lycium chinense root - bark 1 - 5 parts, Atractylodes macrocephala 1 - 5 parts.

[0016] More preferably, the weight parts of each raw material in the traditional Chinese medicine composition are as follows: Atractylodes lancea 9 parts, Anemarrhena asphodeloides 3.6 parts, Glycyrrhiza uralensis 3.6 parts, Poria cocos 9 parts, Astragalus membranaceus 5.4 parts, Houttuynia cordata 6 parts, Morus alba root - bark 8.2 parts, Lepidium apetalum 5.2 parts, Prunus armeniaca 5.4 parts, Tsaoko fruit 6 parts, Gypsum fibrosum 8.2 parts, Pinellia ternata 5.4 parts, Pogostemon cablin 6 parts, Artemisia annua 4.6 parts, Perilla leaf 3.6 parts, Perilla stem 3.6 parts, Lycium chinense root - bark 3.6 parts, Atractylodes macrocephala 3.6 parts.

[0017] The traditional Chinese medicine composition for preventing and treating pulmonary heat cough and asthma in livestock is weighed according to weight parts, respectively pulverized and sieved, and then mixed evenly to prepare a traditional Chinese medicine powder for animal use.

[0018] In the second aspect of the present invention, a preparation method of a traditional Chinese medicine composition for preventing and treating pulmonary heat cough and asthma in livestock is provided. The preparation steps are as follows: (1) Weigh each raw material component according to weight parts. Pretreat Atractylodes lancea, Atractylodes macrocephala, Morus alba root - bark, Tsaoko fruit, and Perilla stem in the traditional Chinese medicine composition by steam explosion process to obtain steam - exploded raw materials; (2) Place the obtained steam - exploded raw materials and the remaining 13 traditional Chinese medicine raw materials in the same extraction tank for water extraction and concentration, and then spray - dry to obtain dry powder; (3) Prepare various traditional Chinese medicine preparations according to the conventional process of veterinary drugs.

[0019] The conditions for the pretreatment by steam explosion process in step (1) are: pressure 1 - 12 MPa, pressure - holding time 10 s - 15 minutes, and temperature controlled at 100 - 250 °C. This process can effectively destroy the cell - wall structure of traditional Chinese medicine, making the active ingredients in the cells more easily dissolved. Compared with the traditional processing method, it can greatly improve the extraction efficiency, thereby enhancing the clinical treatment effect.

[0020] The water extraction and concentration step in step (2) is as follows: Weigh Anemarrhena asphodeloides, Glycyrrhiza uralensis, Poria cocos, Astragalus membranaceus, Houttuynia cordata, Lepidium apetalum, Prunus armeniaca, Gypsum fibrosum, Pinellia ternata, Pogostemon cablin, Artemisia annua, Perilla leaf, and Lycium chinense root - bark according to weight parts, place them in the same extraction tank with the steam - exploded raw materials, and reflux - extract with 6 - 10 times the amount (equivalent to the mass of medicinal materials) of water for 1 - 3 times, 0.5 - 2 hours each time. Combine the extraction liquids and concentrate under reduced pressure to a thick paste with a relative density of 1.10 - 1.20 (55 °C).

[0021] The unique steam explosion pretreatment process combined with the subsequent reasonable preparation process can extract the effective ingredients of traditional Chinese medicine to the greatest extent, improve the bioavailability of the drugs, reduce the dosage of the drugs, reduce the breeding costs, and avoid chemical residues, ensuring the quality and safety of pork, which is in line with the development trend of green breeding.

[0022] The third aspect of the present invention provides the use of the traditional Chinese medicine composition for preventing and treating lung heat, cough and asthma in livestock in medicine for preventing and treating lung heat, cough and asthma in pigs and reducing the risk of abortion in sows.

[0023] The fourth aspect of the present invention provides the use of the traditional Chinese medicine composition for preventing and treating lung heat, cough and asthma in the preparation of drugs for preventing and treating porcine blue ear disease, mycoplasma pneumonia and contagious pleuropneumonia.

[0024] The Chinese veterinary medicine composition of the present invention is scientifically formulated to target the pathogenesis of pig lung heat, cough and asthma and abortion, and has a multi-target synergistic effect. It comprehensively regulates pig body functions from multiple aspects such as clearing heat, moistening the lungs, strengthening the spleen, and calming the fetus, and its preventive and therapeutic effects are significantly better than traditional prescriptions.

[0025] Atractylodes lancea dries dampness, strengthens the spleen and relieves depression, while Poria cocos promotes diuresis, eliminates dampness and calms the mind. The two are used together to target the core pathogenesis of blue ear disease "dampness and toxins blocking the lungs and spleen dysfunction", and the principle of "treating dampness first" is in line with the treatment principle of "when the dampness is gone, the heat is isolated" in "Treatise on Epidemics"; Houttuynia cordata clears heat and detoxifies; Morus alba bark purges the lungs and relieves asthma. Together they form the monarch medicine, and have a rapid therapeutic effect on the pathological stage when the pathogenic factors have entered the qi and heat and toxins block the lungs.

[0026] The seeds of Trichosanthes can purge the lungs and relieve asthma, promote diuresis and reduce swelling, while the bitter almond can relieve cough and asthma, and moisten the intestines and promote bowel movements. Together, they can purge the lungs and relieve asthma, descend qi and promote diuresis, and help the main medicine relieve lung qi stagnation, forming a "promoting upward and unblocking downward" trend, which is in line with the "lung and large intestine are exterior and interior to each other" theory; the seeds of Anemarrhena can clear away heat and purge fire, nourish yin and moisten dryness, while the seeds of Gypsum can clear away heat and purge fire, and relieve restlessness and quench thirst. Both of them clear qi and resolve real heat, and block the heat evil from damaging body fluids. They are the core drug pair variations of Baihu Decoction, targeting high fever and retention, and are all assistant drugs.

[0027] Amomum villosum can dry dampness and warm the middle, stop malaria and eliminate phlegm, while Pinellia ternata can dry dampness and resolve phlegm, reduce adverse effects and stop vomiting. Both of them can clear turbidity with aromatic properties, dry dampness and resolve phlegm, cut off the formation of dampness and turbidity, embody the idea of "treating disease before it occurs", and prevent dampness and turbidity from obscuring the pericardium; Astragalus + Atractylodes macrocephala can replenish Qi and strengthen the exterior, strengthen the body and eliminate evil, prevent excessive attack, and comply with the principle of "eliminating evil without hurting the body"; Artemisia annua + Rehmannia root bark can clear away virtual heat and prevent heat evil from deeply lurking in the yin, and are used as adjuvants for the common low-grade fever in the late stage of blue ear disease.

[0028] Perilla leaves and stems can invigorate the lungs and lead the medicine upward, and they also have the effect of dispelling exterior pathogens and reducing fever; licorice harmonizes the various medicines, alleviates the severe cold nature, nourishes the spleen and replenishes qi, and assists in transportation and transformation, and they are all guiding medicines.

[0029] The whole formula has the effects of clearing away heat and moistening the lungs, relieving cough and asthma, strengthening the spleen and drying dampness, and calming the fetus. It is effective in preventing and treating pig lung heat, cough, asthma and miscarriage.

[0030] The traditional Chinese medicine composition complies with the treatment principle of "diffusing and discharging" in "An Outline of Warm Diseases", has a balanced system of clearing heat without promoting dampness and promoting dampness without promoting heat, and intervenes at multiple targets with traditional Chinese veterinary medicine, reflecting the idea of "intercepting and reversing" the transmission of diseases, and has important value for preventing and controlling the viremia stage.

[0031] The beneficial effects of the present invention are as follows:

[0032] 1. Based on traditional Chinese medicine theory, the present invention scientifically formulates a prescription aiming at the etiological factors and pathogenesis of porcine pulmonary heat cough and abortion, and the various herbs are synergistically formulated to treat both the symptoms and the root causes.

[0033] 2. The raw materials of the traditional Chinese veterinary medicine composition of the present invention are all natural Chinese medicinal herbs, which are green and environmentally friendly, have small toxic and side effects, are not easy to produce drug residues in pigs, are safe for consumption, and meet the development needs of modern animal husbandry for green breeding and antibiotic-free breeding.

[0034] 3. The preparation process is unique, and methods such as steam explosion, water extraction, and spray drying are adopted, which are easy for the extraction of all effective components, operation control, have low costs, are suitable for large-scale industrial production, and are convenient for popularization and application in the pig breeding industry. Specific implementation modes

[0035] The following will further elaborate on the above content through specific implementation modes in the form of examples. However, this should not be understood as limiting the scope of the above subject matter to the following examples. All technologies implemented based on the content of the present invention fall within the scope.

[0036] Example 1 Preparation of soluble powder

[0037] Formulation: 90 kg of Atractylodes lancea, 36 kg of Anemarrhena asphodeloides, 36 kg of Glycyrrhiza uralensis, 90 kg of Poria cocos, 54 kg of Astragalus membranaceus, 60 kg of Houttuynia cordata, 82 kg of Morus alba root bark, 52 kg of Lepidium apetalum, 54 kg of Prunus armeniaca var. ansu, 60 kg of Tsaoko fruit, 82 kg of Gypsum fibrosum, 54 kg of Pinellia ternata, 60 kg of Pogostemon cablin, 46 kg of Artemisia annua, 36 kg of Perilla frutescens leaf, 36 kg of Perilla frutescens stem, 36 kg of Lycium chinense root bark, 36 kg of Atractylodes macrocephala. Preparation method: Accurately weigh each raw material according to the above weight parts. Among them, Atractylodes lancea, Atractylodes macrocephala, Morus alba root bark, Tsaoko fruit, and Perilla frutescens stem are treated by steam explosion process, with a steam explosion pressure of 1.5 MPa and a holding time of 60 s. After steam explosion, add the remaining medicinal materials, add 10 times the amount of water, decoct and extract 2 times, each time for 1.5 hours, combine the extraction solutions, concentrate under reduced pressure to a relative density of 1.2, and spray dry (inlet air temperature 160 °C, outlet air temperature 90 °C) to prepare a soluble powder.

[0038] Example 2 Preparation of powder

[0039] Formulation: Atractylodes lancea 90 kg, Anemarrhena asphodeloides 36 kg, Glycyrrhiza uralensis 36 kg, Poria cocos 90 kg, Astragalus membranaceus 54 kg, Houttuynia cordata 60 kg, Morus alba root bark 82 kg, Lepidium apetalum 52 kg, Armeniaca vulgaris 54 kg, Tsaoko fruit 60 kg, Gypsum fibrosum 82 kg, Pinellia ternata 54 kg, Pogostemon cablin 60 kg, Artemisia annua 46 kg, Perilla frutescens leaf 36 kg, Perilla frutescens stem 36 kg, Lycium chinense root bark 36 kg, Atractylodes macrocephala 36 kg. Preparation method: Weigh the above-mentioned medicinal materials according to the prescription amount, crush them separately, pass through a 24-mesh sieve, and mix evenly.

[0040] Example 3 Preparation of oral liquid.

[0041] Formulation: Atractylodes lancea 80 kg, Anemarrhena asphodeloides 40 kg, Glycyrrhiza uralensis 40 kg, Poria cocos 80 kg, Astragalus membranaceus 50 kg, Houttuynia cordata 60 kg, Morus alba root bark 80 kg, Lepidium apetalum 50 kg, Armeniaca vulgaris 50 kg, Tsaoko fruit 60 kg, Gypsum fibrosum 80 kg, Pinellia ternata 50 kg, Pogostemon cablin 60 kg, Artemisia annua 55 kg, Perilla frutescens leaf 30 kg, Perilla frutescens stem 40 kg, Lycium chinense root bark 40 kg, Atractylodes macrocephala 40 kg. Preparation method: Accurately weigh each raw material according to the above weight parts. Among them, Atractylodes lancea, Atractylodes macrocephala, Morus alba root bark, Tsaoko fruit, and Perilla frutescens stem are treated by steam explosion process, with a steam explosion pressure of 2.5 MPa and a holding time of 80 s. After the steam explosion is completed, add the remaining medicinal materials, add 10 times the amount of water, decoct and extract 2 times, each time for 2 hours, combine the extraction liquid, concentrate under reduced pressure to a relative density of 1.2, filter with a 300-mesh filter cloth, formulate into a concentration of 1.5 kg of raw medicinal materials per kg of medicinal liquid, mix evenly, sterilize with flowing steam for 30 minutes, and fill into bottles to obtain the product.

[0042] Example 4 Preparation of granules

[0043] Formulation: Atractylodes lancea 60 kg, Anemarrhena asphodeloides 30 kg, Glycyrrhiza uralensis 30 kg, Poria cocos 90 kg, Astragalus membranaceus 50 kg, Houttuynia cordata 40 kg, Morus alba root bark 60 kg, Lepidium apetalum 40 kg, Armeniaca vulgaris 40 kg, Tsaoko fruit 60 kg, Gypsum fibrosum 80 kg, Pinellia ternata 40 kg, Pogostemon cablin 50 kg, Artemisia annua 30 kg, Perilla frutescens leaf 30 kg, Perilla frutescens stem 30 kg, Lycium chinense root bark 30 kg, Atractylodes macrocephala 30 kg. Preparation method: Accurately weigh each raw material according to the above weight parts. Among them, Atractylodes lancea, Atractylodes macrocephala, Morus alba root bark, Tsaoko fruit, and Perilla frutescens stem are treated by steam explosion process, with a steam explosion pressure of 2.0 MPa and a holding time of 120 s. After the steam explosion is completed, add the remaining medicinal materials, add 10 times the amount of water, decoct and extract 2 times, each time for 1.5 hours, combine the extraction liquid, concentrate under reduced pressure to a relative density of 1.2, filter with a 300-mesh filter cloth, mix evenly with 50 kg of dextrin and 60 kg of powdered sugar, use 60% ethanol as a binder to make granules, dry, size the granules, and package them to obtain the product.

[0044] Example 5 Preparation of soluble powder

[0045] Formulation: Atractylodes lancea 50 kg, Anemarrhena asphodeloides 10 kg, Glycyrrhiza uralensis 10 kg, Poria cocos 50 kg, Astragalus membranaceus 20 kg, Houttuynia cordata 30 kg, Mori cortex 40 kg, Lepidium apetalum 20 kg, Armeniaca vulgaris var. ansu 20 kg, Tsaoko fruit 30 kg, Gypsum fibrosum 40 kg, Pinellia ternata 20 kg, Pogostemon cablin 30 kg, Artemisia annua 20 kg, Perilla frutescens leaf 10 kg, Perilla frutescens stem 10 kg, Lycii radicis cortex 10 kg, Atractylodes macrocephala 10 kg. Preparation method: Weigh each raw material accurately according to the above weight parts. Among them, Atractylodes lancea, Atractylodes macrocephala, Mori cortex, Tsaoko fruit, and Perilla frutescens stem are processed by steam explosion technology with a steam explosion pressure of 3.5 MPa and a holding time of 60 s. After the steam explosion, add the remaining medicinal materials, add 10 times the amount of water, decoct and extract 2 times, each time for 1.5 hours, combine the extraction liquid, concentrate under reduced pressure to a relative density of 1.2, and spray dry (inlet air temperature 160 °C, outlet air temperature 90 °C) to make a soluble powder.

[0046] Example 6 Preparation of soluble powder

[0047] Formulation: Atractylodes lancea 100 kg, Anemarrhena asphodeloides 50 kg, Glycyrrhiza uralensis 50 kg, Poria cocos 100 kg, Astragalus membranaceus 70 kg, Houttuynia cordata 70 kg, Mori cortex 100 kg, Lepidium apetalum 70 kg, Armeniaca vulgaris var. ansu 70 kg, Tsaoko fruit 70 kg, Gypsum fibrosum 100 kg, Pinellia ternata 70 kg, Pogostemon cablin 70 kg, Artemisia annua 60 kg, Perilla frutescens leaf 50 kg, Perilla frutescens stem 50 kg, Lycii radicis cortex 50 kg, Atractylodes macrocephala 50 kg. Preparation method: Weigh each raw material accurately according to the above weight parts. Among them, Atractylodes lancea, Atractylodes macrocephala, Mori cortex, Tsaoko fruit, and Perilla frutescens stem are processed by steam explosion technology with a steam explosion pressure of 3.5 MPa and a holding time of 60 s. After the steam explosion, add the remaining medicinal materials, add 10 times the amount of water, decoct and extract 2 times, each time for 1.5 hours, combine the extraction liquid, concentrate under reduced pressure to a relative density of 1.2, and spray dry (inlet air temperature 160 °C, outlet air temperature 90 °C) to make a soluble powder.

[0048] Comparative Example 1 Preparation of soluble powder

[0049] The formulation is the same as that in Example 1, and the preparation process is: After all of Atractylodes lancea, Anemarrhena asphodeloides, Glycyrrhiza uralensis, Poria cocos, Astragalus membranaceus, Houttuynia cordata, Mori cortex, Lepidium apetalum, Armeniaca vulgaris var. ansu, Tsaoko fruit, Gypsum fibrosum, Pinellia ternata, Pogostemon cablin, Artemisia annua, Perilla frutescens leaf, Perilla frutescens stem, Lycii radicis cortex, and Atractylodes macrocephala are processed by steam explosion technology, they are extracted with water. The steam explosion and water extraction process conditions are the same as those in Example 1.

[0050] Comparative Example 2

[0051] Prepare a traditional Chinese medicine preparation using the formulation and process in Example 3 of Patent CN116077578A.

[0052] Example 7 Clinical efficacy test for prevention and treatment of porcine reproductive and respiratory syndrome

[0053] 1. Test drugs

[0054] 30 kg each of the products of Example 1, Examples 5 - 6, and Comparative Examples 1 - 2 of the present invention, control group products, marker pens, blood collection consumables (syringes, vacuum blood collection tubes, 2.0 ml EP tubes, etc.).

[0055] 2. Experimental methods

[0056] 2.1 Grouping of test pig herds

[0057] In a certain 5000 - sow farm in Fuzhou, Fujian, it has always been positive for porcine reproductive and respiratory syndrome (PRRS), and pulmonary heat, cough, and asthma are common in weaned pigs. On July 21, 2024, 298 weaned piglets in farrowing house No. 10 were transferred to nursery house 1 - 1. They were divided into 6 groups according to the east - west - middle and north - south of the feeding line, as follows.

[0058] Table 2 Grouping and drug administration

[0059] 。

[0060] 2.2 Collecting samples after grouping

[0061] Before and after the test, 10 pig blood samples were randomly collected from each of the product groups of Example 1, Example 5, Example 6, Comparative Example 1, and Comparative Example 2, and 10 pig blood samples were randomly collected from the control group, for a total of 60 blood samples; the ear tags were marked as 01 - 10, 11 - 20, 21 - 30, 31 - 40, 41 - 50, 51 - 60; the ear tags of the control group were marked as 51 - 60. At the same time, the S / P value of PRRS antibody and the detection of PRRSV pathogen were carried out to evaluate the current PRRSV infection level of the test pig farm and ensure that they were at the same infection level.

[0062] 3. Treatment and drug administration

[0063] The product group of Example 1 was administered for treatment simultaneously with the product groups of Example 5, Example 6, Comparative Example 1, and Comparative Example 2. Each group of products was 3 kg / ton and used for 14 consecutive days.

[0064] Forty - eight pigs in the control group were administered 2 kg / ton of 20% tilmicosin premix + 1 kg / ton of 10% compound amoxicillin in the feed and continuously administered for 14 days.

[0065] 4. Experimental results

[0066] Collection of production data and evaluation of experimental data for the treatment experiment of nursery and growing pigs.

[0067] 1. The S / P value of PRRS antibody and the PRRSV pathogen value before and after the test.

[0068] Statistically analyze the S / P values of porcine reproductive and respiratory syndrome (PRRS) antibodies and PRRSV pathogen values before and after the tests for each experimental group and the control group, calculate the average values and dispersions of the test reports, and the statistical results are shown in the following table.

[0069] Table 3 Comparative Analysis of Test Data for the Experimental Group and the Control Group

[0070] 。

[0071] Table 4 Record Form for Barn Rounds during the Test

[0072] 。

[0073] Note: The weak and sick pigs include, but are not limited to, pigs showing symptoms such as coughing, shortness of breath, emaciation, lameness, anorexia, listlessness, and stunted pigs. Take a video of the pig group every day, about 1 minute in length, and the person taking the video should state the time, location, and the state of the pig group.

[0074] The pig selection refers to the operation process in which breeders or professional technical personnel conduct a comprehensive and detailed observation of a pen of pigs, identify individuals with health problems or abnormal growth and development based on multiple indicators such as the appearance signs, behavior manifestations, and mental states of the pigs, and select them from the original breeding group and transfer them to a separate pen for special breeding.

[0075] The purpose of pig selection is to focus on and manage pigs with potential problems, so as to timely detect and handle diseases, improve their health conditions, and at the same time avoid possible disease transmission, ensuring the health and production efficiency of the entire pig group.

[0076] 5. Experimental Conclusions

[0077] From the analysis of the S / P values of PRRS antibodies and PRRSV pathogens, compared with before the test, the S / P values of PRRS antibodies and PRRSV pathogens in the blood samples of the product group of Example 1 decreased significantly after the test; the product groups of Example 5, Example 6, Comparative Example 1, and Comparative Example 2 had certain effects, but the cure rates were significantly different from that of the product of Example 1. The control group had a tendency to recover, but far less than that of the traditional Chinese medicine group. The product group of Example 1 was slightly better than the product groups of Example 5 and Example 6, but the difference was not significant; the product groups of the embodiments of the present invention were significantly different from the product groups of Comparative Example 1, Comparative Example 2, and the control group; the product groups of Comparative Example 1 and Comparative Example 2 were not as good as other experimental groups in terms of laboratory test indicators, but were better than the control group.

[0078] Based on the comprehensive mortality and clinical observations, it can be concluded that in Examples 1, 5, and 6 of the present invention, the veterinary medicine composition has significantly better curative effects in treating porcine pulmonary heat cough, asthma, etc. than the control group and the comparative group, can effectively relieve the symptoms of pigs, improve the cure rate, and fully demonstrate the good application prospects of the traditional Chinese veterinary medicine composition of the present invention in preventing and treating porcine reproductive and respiratory syndrome.

[0079] Example 8 Clinical Trial on Preventing and Treating Abortion of Gestating Sows

[0080] To verify the clinical efficacy of the traditional Chinese veterinary medicine composition of the present invention, the following tests were conducted:

[0081] Test animals: Select a pig farm with positive mycoplasma and porcine reproductive and respiratory syndrome virus detections. Select 240 gestating sows showing signs of abortion and randomly divide them into 6 groups, with 40 sows in each group.

[0082] 1. Test drugs and dosage

[0083] Experimental group 1: The traditional Chinese veterinary medicine composition prepared in Example 1 of the present invention, used at a dosage of 3 kg / ton of feed for 14 consecutive days.

[0084] Experimental group 2: The traditional Chinese veterinary medicine composition prepared in Example 5 of the present invention, used at a dosage of 3 kg / ton of feed for 14 consecutive days.

[0085] Experimental group 3: The traditional Chinese veterinary medicine composition prepared in Example 6 of the present invention, used at a dosage of 3 kg / ton of feed for 14 consecutive days.

[0086] Experimental group 4: The traditional Chinese veterinary medicine composition prepared in Comparative Example 1 of the present invention, used at a dosage of 3 kg / ton of feed for 14 consecutive days.

[0087] Experimental group 5: The traditional Chinese veterinary medicine composition prepared in Comparative Example 2 of the present invention, used at a dosage of 3 kg / ton of feed for 14 consecutive days.

[0088] Control group: Commercially available 20% tilmicosin premix at 2 kg + 10% compound amoxicillin powder at 1 kg / ton of feed, used for 14 consecutive days.

[0089] 2. Test method

[0090] Observe and record the changes in symptoms such as coughing, wheezing, and body temperature of pigs, record the abortion situation of sows, and statistically analyze the success rate of fetal protection and the health status of the piglets born.

[0091] 3. Curative effect judgment criteria

[0092] Cured: Symptoms such as coughing and wheezing completely disappear, body temperature returns to normal, food intake and water intake return to normal; the sow does not have an abortion, gives birth normally at the expected date of delivery, and the piglets born are healthy and lively.

[0093] Effective: Symptoms such as cough and asthma are significantly reduced, body temperature is basically normal, food intake and water intake are improved; significantly fewer sows have miscarriages, give normal birth at the expected due date, and more than 90% of the piglets born are healthy and lively.

[0094] Ineffective: Symptoms show no obvious improvement or get worse; sows have miscarriages and the proportion exceeds 3%.

[0095] 4. Test Results

[0096] The symptoms of different test groups were statistically analyzed respectively, the differences between different groups were analyzed, and the efficacy was evaluated. The statistics are shown in the following table.

[0097] Table 5 Symptom Improvement and Efficacy of Different Experimental Groups

[0098] 。

[0099] The test results show that the traditional Chinese veterinary medicine composition of the present invention has significantly better curative effects than the control group in treating pulmonary heat cough and asthma in pigs and preventing sow miscarriages, can effectively relieve the symptoms of pigs, improve the cure rate, the success rate of protecting the fetus, and the health rate of the piglets born, and fully shows the good application prospects of the traditional Chinese veterinary medicine composition of the present invention in preventing and treating pulmonary heat cough and asthma and miscarriages in pigs.

[0100] Although the specific implementation manners of the present invention have been described above, those skilled in the art of this technology should understand that the specific embodiments we described are illustrative rather than used to limit the scope of the present invention. Equivalent modifications and variations made by those skilled in the art of this technology in accordance with the spirit of the present invention should be covered by the scope protected by the claims of the present invention.

Claims

1. A traditional Chinese medicine composition for preventing and treating pulmonary heat cough and asthma in livestock, characterized in that: The traditional Chinese medicine composition is composed of the following raw materials: Atractylodes lancea, Anemarrhena asphodeloides, Glycyrrhiza uralensis, Poria cocos, Astragalus membranaceus, Houttuynia cordata, Morus alba root bark, Lepidium apetalum, Prunus armeniaca var. ansu, Tsaoko fruit, Gypsum fibrosum, Pinellia ternata, Pogostemon cablin, Artemisia annua, Perilla leaf, Perilla stem, Lycium chinense Mill., and Atractylodes macrocephala; the traditional Chinese medicine composition is prepared from the following raw materials according to the weight parts ratio: 5-10 parts of Atractylodes lancea, 1-5 parts of Anemarrhena asphodeloides, 1-5 parts of Glycyrrhiza uralensis, 5-10 parts of Poria cocos, 2-7 parts of Astragalus membranaceus, 3-7 parts of Houttuynia cordata, 4-10 parts of Morus alba root bark, 2-7 parts of Lepidium apetalum, 2-7 parts of Prunus armeniaca var. ansu, 3-7 parts of Tsaoko fruit, 4-10 parts of Gypsum fibrosum, 2-7 parts of Pinellia ternata, 3-7 parts of Pogostemon cablin, 2-6 parts of Artemisia annua, 1-5 parts of Perilla leaf, 1-5 parts of Perilla stem, 1-5 parts of Lycium chinense Mill., and 1-5 parts of Atractylodes macrocephala; The preparation method of the traditional Chinese medicine composition for preventing and treating pulmonary heat cough and asthma in livestock is as follows: (1) Weigh each raw material according to the weight parts, and perform pretreatment on 5 traditional Chinese medicines, namely Atractylodes lancea, Atractylodes macrocephala, Morus alba root bark, Tsaoko fruit, and Perilla stem, by using the steam explosion process to obtain the steam-exploded raw materials; (2) Place the steam-exploded raw materials obtained in step (1) and other traditional Chinese medicine raw materials in the same extraction tank for water extraction and concentration, and then perform spray drying to obtain dry powder; (3) Prepare various traditional Chinese medicine preparations from the dry powder in step (2) according to the conventional process of veterinary drug preparations.

2. The traditional Chinese medicine composition according to claim 1, wherein: The traditional Chinese medicine composition is prepared from the following raw materials according to the weight parts ratio: 9 parts of Atractylodes lancea, 3.6 parts of Anemarrhena asphodeloides, 3.6 parts of Glycyrrhiza uralensis, 9 parts of Poria cocos, 5.4 parts of Astragalus membranaceus, 6 parts of Houttuynia cordata, 8.2 parts of Morus alba root bark, 5.2 parts of Lepidium apetalum, 5.4 parts of Prunus armeniaca var. ansu, 6 parts of Tsaoko fruit, 8.2 parts of Gypsum fibrosum, 5.4 parts of Pinellia ternata, 6 parts of Pogostemon cablin, 4.6 parts of Artemisia annua, 3.6 parts of Perilla leaf, 3.6 parts of Perilla stem, 3.6 parts of Lycium chinense Mill., and 3.6 parts of Atractylodes macrocephala.

3. The traditional Chinese medicine composition according to claim 1, wherein: The conditions of the steam explosion process described in step (1) of the preparation are: pressure 1-12 MPa, pressure holding time 10 s-15 minutes, and the temperature is controlled at 100-250 °C.

4. The traditional Chinese medicine composition according to claim 1, wherein: The steps of the water extraction and concentration described in step (2) of the preparation are to reflux and extract 1-3 times with 6-10 times the amount of water equivalent to the mass of the medicinal materials, each time for 0.5-2 hours, combine the extraction solutions, and perform decompression concentration to obtain a thick paste with a relative density of 1.10-1.20 at 55 °C.

5. Use of a traditional Chinese medicine composition for preventing and treating pulmonary heat cough and asthma in livestock according to any one of claims 1-2 in the preparation of a drug for preventing and treating pulmonary heat cough and asthma in pigs and reducing the risk of miscarriage symptoms in sows.

6. Use of a traditional Chinese medicine composition for preventing and treating pulmonary heat cough and asthma in livestock according to any one of claims 1-2 in the preparation of a drug for preventing and treating porcine reproductive and respiratory syndrome, mycoplasma pneumonia, and contagious pleuropneumonia.