Chinese herbal medicine composition capable of repairing skin and preparation method thereof

Abstract

The invention relates to the technical field of traditional Chinese medicine compositions, and discloses a Chinese herbal medicine composition capable of repairing skin and a preparation method thereof, the Chinese herbal medicine composition comprises the following raw materials by weight: 8-15 parts of dahurian angelica root, 8-15 parts of giant typhonium rhizome, 20-40 parts of mung bean, 12-24 parts of Chinese pulsatilla root, 5-10 parts of gentian, 6-12 parts of cortex dictamni, 9-17 parts of common andrographis herb, 8-18 parts of corydalis tuber, 10-20 parts of catclaw buttercup root, 15-25 parts of Chinese lobelia, 3-10 parts of reed rhizome, and 12-24 parts of witch hazel. The problems of skin irritation, drug resistance, easy relapse after drug withdrawal, low patient compliance and the like in the existing western medicine treatment are solved.

Description

Technical Field

[0001] The present invention relates to the technical field of Chinese medicine compositions, and in particular to a Chinese herbal medicine composition capable of repairing skin and a preparation method thereof. Background Art

[0002] Acne, commonly known as pimples or blackheads, is a chronic inflammatory skin disease of the hair follicles and sebaceous glands. It is prone to occur in areas rich in sebaceous glands such as the face, chest, and back, forming papules, blackheads, pustules, nodules, or cysts. It mostly occurs in young men and women aged 15-30. About 80% of adolescents suffer from acne to varying degrees. Severe cases are accompanied by scar formation, which affects the patient's appearance. Even worse, it has a serious impact on the psychological and social aspects of adolescents.

[0003] At present, Western medicine is mainly used to treat acne in clinical practice. Common drugs include: ① Anti-androgen drugs, which mainly reduce facial sebum secretion by inhibiting the synthesis of androgen in the body. ② Anti-abnormal keratinization drugs of hair follicle ducts, such as retinoic acid drugs. ③ Anti-acne bacteria drugs, such as benzoyl peroxide, erythromycin, clindamycin, tetracycline, etc. Although Western medicine treatment can achieve certain effects in the short term, there are also major problems. For example, the use of anti-androgen drugs may cause endocrine disorders; the use of rapid bactericidal and acne-removing drugs will cause dry skin, peeling, erythema and enlarged pores. All these indicate that the current clinical treatment of acne drugs has limited efficacy, and there is still a lack of a safe and effective treatment method, so that many acne patients need long-term repeated treatment, but eventually form obvious marks, which seriously affect the patient's appearance and self-confidence.

[0004] With the vigorous development of traditional Chinese medicine, people favor natural products, and traditional Chinese medicine for the treatment of acne has been increasingly valued and recognized. Nowadays, the application of herbal medicine products to treat acne has achieved good results. For example, patent No. CN201810132362.4 discloses a Chinese medicine composition for the treatment of acne and its preparation method and use. The main raw materials of the composition are loquat leaves, scutellaria, dandelion, forsythia, trichosanthes, honeysuckle, salvia miltiorrhiza, Eclipta prostrata, Ligustrum lucidum and licorice. After washing and drying, the above raw medicinal materials are prepared into fine powder using ultrafine grinding equipment and added to common matrices to prepare various external preparations or extracted by conventional extraction methods and prepared into oral preparations for use. The composition of the invention has a reasonable formula and clear efficacy. It has a good therapeutic effect on acne caused by various reasons, with quick effect, low cost, high cure rate and low recurrence rate.

[0005] For another example, CN202110732664.7 discloses a Chinese medicine composition, a Chinese medicine extract, a Chinese medicine preparation, a preparation method and an application. The Chinese medicine composition includes: 100-300 parts of honeysuckle, 100-300 parts of Sophora japonica, 100-300 parts of Lithospermum officinale, 200-400 parts of Forsythia suspensa, 100-300 parts of Scutellaria baicalensis, 100-300 parts of Viola yedoensis, 100-300 parts of Gleditsia sinensis, 100-300 parts of Prunella vulgaris, 400-800 parts of Coix seeds, 100-300 parts of Atractylodes macrocephala, 200-400 parts of Portulaca oleracea, 150-350 parts of Hawthorn, 100-300 parts of Salvia miltiorrhiza, 100-300 parts of Paeonia suffruticosa, and 80-200 parts of Chrysanthemum. The Chinese medicine preparation prepared by using the Chinese medicine composition is used for treating acne, seborrheic dermatitis, rosacea and rosacea-like dermatitis, is simple and easy to use, has good tolerance, has no side effects, and the content of chlorogenic acid is above 0.5 mg / g, which can ensure the stability of the efficacy of the Chinese medicine preparation.

[0006] According to the pathogenesis and treatment principles of acne, the inventor screened out the raw materials in the Chinese medicine composition of the present invention and adjusted the most suitable raw material dosage. The composition of the present invention has a reasonable formula and clear efficacy. It has a good therapeutic effect on acne caused by various reasons, with quick effect, low cost, high cure rate and low recurrence rate. Summary of the invention

[0007] Therefore, in view of the above, the present invention provides a Chinese herbal medicine composition capable of repairing skin and a preparation method thereof, so as to solve the problems of skin irritation, drug resistance, easy recurrence after drug withdrawal and low patient compliance in existing western medicine treatments.

[0008] The present invention also provides the use of the above-mentioned Chinese herbal medicine composition capable of repairing skin in the preparation of medicines or cosmetics for preventing and / or treating acne.

[0009] To achieve the above object, the present invention is achieved through the following technical solutions:

[0010] A Chinese herbal medicine composition capable of repairing skin comprises the following raw materials in parts by weight: 8-15 parts of angelica dahurica, 8-15 parts of white aconite root, 20-40 parts of mung bean, 12-24 parts of pulsatilla, 5-10 parts of gentian, 6-12 parts of dittany, 9-17 parts of andrographis paniculata, 8-18 parts of corydalis, 10-20 parts of cat's claw, 15-25 parts of lobelia, 3-10 parts of reed root and 12-24 parts of witch hazel.

[0011] A further improvement is that the raw material composition includes the following parts by weight: 8-12 parts of angelica dahurica, 10-15 parts of white aconite root, 24-36 parts of mung bean, 14-24 parts of pulsatilla, 6-9 parts of gentian, 7-11 parts of dittany, 10-16 parts of andrographis paniculata, 10-18 parts of corydalis, 12-20 parts of cat's claw, 16-24 parts of lobelia, 6-10 parts of reed root, and 14-22 parts of witch hazel.

[0012] A further improvement is that the raw materials are composed of the following parts by weight: 10 parts of Angelica dahurica, 12 parts of Rhizoma Cyperi, 30 parts of Mung Bean, 20 parts of Pulsatilla scabra, 8 parts of Gentiana scabra, 9 parts of Dictamni Cortex, 12 parts of Andrographis paniculata, 15 parts of Corydalis yanhusuo, 16 parts of Cat's Claw, 20 parts of Lobelia, 8 parts of Phragmites australis, and 18 parts of Hamamelis virginiana.

[0013] The preparation method of the above-mentioned Chinese herbal medicine composition capable of repairing skin comprises the following steps:

[0014] S1. Weigh angelica dahurica, white aconite root, mung bean, pulsatilla, gentian, Dictamni cortex, andrographis paniculata, Corydalis yanhusuo, cat's claw, lobelia, reed root, and Hamamelis virginiana according to the weight of the formula, mix and crush to 60-100 mesh, add 80wt% ethanol solution of 10-15 times of raspberry, soak for 1-2h, then perform ultrasonic assisted extraction for 30-60min, the ultrasonic extraction temperature is 40-50℃, filter after the extraction to obtain a first filtrate and a first filter residue; add 80wt% ethanol solution to the first filter residue for reflux extraction, and filter to obtain a second filtrate and a second filter residue;

[0015] S2. Combine the filtrates and concentrate under reduced pressure, then extract and decolorize with an equal volume of ethyl acetate, and then extract the aqueous phase with an equal volume of n-butanol; then rotary evaporate the n-butanol phase and concentrate to obtain the product.

[0016] A further improvement is that the solid-liquid ratio of the first filter residue to the ethanol solution is 1:8-12, and the extraction time is 1.5-2.5 hours.

[0017] A further improvement is: adding ethanol solution to the second filter residue, further reflux extraction, and filtering to obtain a third filtrate.

[0018] A further improvement is that the solid-liquid ratio of the second filter residue to the ethanol solution is 1:6-9, and the extraction time is 1-2 hours.

[0019] The present invention also provides the use of the above-mentioned Chinese herbal medicine composition capable of repairing skin in the preparation of medicines or cosmetics for preventing and / or treating acne.

[0020] A further improvement is that the medicine or cosmetic includes powder, ointment, gel, spray or liniment. That is, the active ingredients of the medicine of the present invention can be added with various conventional excipients required for preparing different dosage forms, such as lubricants, adhesives, etc., and prepared into any commonly used external dosage form, such as powder, ointment, gel, spray or liniment, etc., by conventional Chinese medicine preparation methods.

[0021] The common pharmacological effects of the Chinese medicinal raw materials selected in the present invention in this field are as follows:

[0022] Angelica dahurica is warm in nature, pungent in taste, and belongs to the lung and stomach meridians. It has the effects of dispelling wind and relieving exterior symptoms, reducing swelling and draining pus, and clearing the orifices. Acne is prone to occur on the head and face, and the head and face are the meeting points of all yang. Angelica dahurica is pungent in taste, can disperse and move, belongs to the lung meridian, and is also a meridian-leading drug for the Yangming meridian, so it can reach the clear orifices, clear the lungs, reduce swelling and drain pus. Modern pharmacological studies have shown that Angelica dahurica has antibacterial, antioxidant, anti-inflammatory, neuroprotective and analgesic effects, and mainly contains a variety of chemical components such as coumarins, volatile oils, glycosides, and alkaloids.

[0023] Radix et Rhizoma Aconiti Lateralis, pungent in taste, sweet, warm in nature, toxic, enters stomach and liver meridians. Radix et Rhizoma Aconiti Lateralis has the effects of dispelling wind and phlegm, removing toxins and dispersing stagnation, helps to dissipate the local induration and inflammation of acne, improves the acne symptoms such as papules, pustules caused by heat-toxic phlegm, and promotes the swelling of acne to disappear. Radix et Rhizoma Aconiti Lateralis also has an anti-inflammatory effect, for the redness and swelling, pain of acne inflammation period, can play a certain mitigation effect, alleviate the stimulation of inflammatory reaction to skin, and contribute to the recovery of skin inflammation.

[0024] Pulsatilla, bitter in taste, cold in nature, enters the stomach and large intestine meridians. It has the effects of clearing away heat and detoxifying, cooling blood and stopping dysentery. Pulsatilla has a certain inhibitory effect on Propionibacterium acnes, it can clear away heat and detoxify, and relieve the inflammatory response caused by acne.

[0025] Gentiana scabra is bitter and cold in nature. It enters the liver and gallbladder meridians and is used to clear away heat and dampness, and to relieve liver and gallbladder fire. It is used for damp-heat jaundice, vaginal swelling and itching, leucorrhea, eczema itching, liver-fire red eyes, tinnitus and deafness, flank pain and bitter mouth, strong middle, convulsions. Gentiana scabra has good heat-clearing and detoxifying effects, can inhibit the release of inflammatory mediators in the body, and reduce inflammatory reactions.

[0026] Dictamni peel is bitter in taste and cold in nature. It enters the spleen, stomach and bladder meridians. It has the effects of clearing away heat and dampness, dispelling wind and detoxifying. It is mainly used for clearing heat and dampness, purging fire and detoxifying, dispelling wind and relieving itching, jaundice and red urine, and damp-heat pain; it is used externally for eczema, scabies, skin itching, skin ulcers, and yellow water dripping. Modern medical research and clinical application have proved that Dictamni peel contains dittanic acid, dittanylactone, sitosterol, choline, and osmoticum. It has different degrees of inhibitory effects on a variety of pathogenic fungi. Dictamni peel also has an antipyretic effect. Dictamni peel can regulate the oil balance of the skin, inhibit excessive secretion of sebaceous glands, and is also helpful to improve symptoms such as skin itching caused by excessive secretion of sebaceous glands.

[0027] Andrographis paniculata is bitter and cold in nature. It enters the heart, lung, large intestine and bladder meridians. It has the effects of clearing away heat and detoxifying, cooling blood and reducing swelling, and drying dampness. It is used to treat colds and fever, sore throat, mouth sores, coughs, diarrhea, dysentery, hot and painful stranguria, carbuncles, sores, etc.

[0028] Corydalis yanhusuo, with a pungent and bitter taste and warm nature, enters the heart, liver and spleen meridians. Corydalis yanhusuo has a significant effect of promoting blood circulation and relieving pain, and has a good effect on relieving various pain symptoms caused by blood stasis, such as chest pain, heart pain, stomach pain, dysmenorrhea, etc. Corydalis yanhusuo also has the effect of anti-inflammatory and swelling, and has a certain therapeutic effect on swelling, pain and other symptoms caused by inflammation.

[0029] Cat's claw is sweet and spicy, warm in nature, and enters the liver and lung meridians. Cat's claw has the effect of resolving phlegm and dispersing nodules. It can help dissolve phlegm and dampness in the body. It has a certain improvement effect on acne caused by phlegm and dampness accumulation, such as hard and nodular acne, and can gradually dissipate the nodules. Cat's claw can detoxify and reduce swelling. Acne is, to some extent, a manifestation of "toxic swelling" of the skin. When acne appears red, swollen, and painful, cat's claw can play its detoxifying role, reduce the local inflammatory response of acne, relieve the symptoms of redness, swelling, heat and pain, and promote the recovery of acne.

[0030] Lobelia has a sweet and light taste and a cold nature. It is suitable for the heart, small intestine and lung meridians. Lobelia can remove heat and toxins from the body and reduce inflammation caused by heat and toxins in acne, such as relieving symptoms such as redness, swelling, pain and pustules of acne. Lobelia has a diuretic and detumescent effect, which can promote the body's water metabolism and help excrete toxins. From the perspective of traditional Chinese medicine, the occurrence of acne is related to the accumulation of moisture and toxins in the body. By promoting diuresis, it can remove some of the pathological factors that cause acne and play an auxiliary role in the treatment of acne. When these toxins are discharged from the body, the toxin pressure on the skin is reduced, which is helpful for improving the condition of the skin damaged by toxin accumulation and enabling the skin to better repair itself. Lobelia contains a variety of active ingredients with anti-inflammatory and antibacterial effects. For skin damage, such as small wounds caused by acne damage and abrasions, it can inhibit the growth of bacteria at the wound, reduce inflammatory reactions, create a good healing environment for the wound, thereby promoting wound healing and reducing the risk of infection.

[0031] Reed root, sweet in taste, cold in nature, belongs to the lung and stomach meridians. Reed root can clear away heat and purge fire, and has a good therapeutic effect on acne caused by excessive heat in the lung and stomach. For example, when acne manifests as redness, swelling, heat and pain, and pustules, reed root can clear the heat of the lung and stomach, reduce the inflammatory response of acne, and relieve the redness, swelling, and pain of the skin. Reed root has a detoxifying effect. It can help the body excrete toxins. For acne, which is an inflammation of the hair follicles and sebaceous glands of the skin, reed root can play a detoxifying role and promote the discharge of pus. For acne that has already become purulent, reed root is conducive to the healing of the sore and reduces the possibility of acne leaving scars. The diuretic effect of reed root can promote the metabolism of water in the body. From the perspective of traditional Chinese medicine, the occurrence of acne is also related to the accumulation of moisture in the body. Reed root can assist in the treatment of acne by promoting water and removing moisture, reducing the impact of moisture on the skin.

[0032] Witch hazel is sweet and astringent in taste, neutral in nature, and enters the liver and spleen meridians. Witch hazel has astringent, soothing and anti-inflammatory effects. Its extracts are often used in skin care products. They can regulate the secretion of skin oil, shrink pores, make facial oil distribution more even, and prevent local oil accumulation from clogging pores. For acne scars after inflammation, witch hazel can reduce the inflammatory response of the local skin and relieve the redness and swelling of the acne scars. At the same time, witch hazel extract can promote skin metabolism and help skin renewal, so that the skin cells in the acne scar area can regenerate faster, gradually fade the acne scars, and improve the overall texture of the skin, making the skin smoother.

[0033] Mung bean tastes sweet, is cold in nature, and enters the heart and stomach meridians. It has the effect of clearing away heat and detoxifying. First of all, mung bean can remove heat and toxins from the body, reduce inflammatory reactions, and have a certain improvement effect on acne caused by internal heat, damp heat and other factors. Secondly, mung bean also has a diuretic effect. It can promote water metabolism in the body and help excrete toxins. When toxins in the body are excreted through urine and other means, it can reduce the accumulation of toxins on the skin, thereby playing a positive auxiliary role in the treatment of acne. In addition, mung beans are rich in protein, B vitamins, and minerals such as calcium and iron. These nutrients are of great significance to maintaining the normal metabolism and physiological functions of the skin, helping to repair damaged skin and promote the recovery of acne.

[0034] By adopting the above technical solution, the beneficial effects of the present invention are:

[0035] The present invention studies the pathogenesis of acne on the basis of syndrome differentiation and treatment, combines various Chinese herbal medicines, and produces products with greatly improved efficacy and safety, avoiding the irritation of chemical drugs to the skin and the drug resistance problem caused by long-term use, and the patient compliance is high.

[0036] Specifically, the Chinese herbal medicine composition has the effects of clearing away heat and dampness, removing toxins and eliminating carbuncle, and promoting blood circulation and removing blood stasis. By clearing away heat and drying dampness, the damp and hot environment in the body is improved, and the accumulation of damp and hot evil in the skin is reduced. By promoting blood circulation and removing blood stasis, the circulation of qi and blood can be regulated, the blood circulation of the skin can be improved, and it is helpful to remove local heat toxins and metabolic waste. Detoxification and elimination of carbuncle can promote the discharge of pus, avoid pus accumulation in the skin, and reduce the risk of inflammation spread and scar formation. In addition, the Chinese herbal medicine composition can also inhibit the large-scale secretion of the hair follicle sebaceous glands in the body, and effectively balance the distribution of facial oils, avoid a large amount of oils clogging the pores, thereby curbing the growth and reproduction of Propionibacterium acnes in an anaerobic environment. DETAILED DESCRIPTION

[0037] The following will describe the implementation methods of the present invention in detail in conjunction with specific embodiments, so that the implementation process of how the present invention applies technical means to solve technical problems and achieve technical effects can be fully understood and implemented accordingly.

[0038] Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the reagents and products used are all commercially available. The sources, trade names and components of the reagents used are indicated when they first appear.

[0039] Example 1

[0040] A Chinese herbal medicine composition for repairing skin comprises the following raw materials in parts by weight: 8 parts of angelica dahurica, 10 parts of white aconite root, 24 parts of mung bean, 14 parts of pulsatilla, 6 parts of gentian, 7 parts of dittany, 10 parts of andrographis paniculata, 10 parts of corydalis, 12 parts of cat's claw, 16 parts of lobelia, 6 parts of reed root, and 14 parts of witch hazel.

[0041] The above-mentioned Chinese herbal medicine composition capable of repairing skin is made into an ointment and applied to prevent and / or treat acne. The preparation method of the ointment comprises the following steps:

[0042] (1) According to the weight proportion of the formula, Angelica dahurica, Radix Aconiti Lateralis, Mung Bean, Pulsatilla scabra, Gentiana scabra, Cortex Dictamni, Andrographis paniculata, Corydalis yanhusuo, Herba Cat's Claw, Herba Lobeliae, Rhizoma Phragmites, and Hamamelis Virginiana are weighed, the mixture is crushed into 60 meshes, 10 times the amount of 80 wt % ethanol solution of raspberry is added, the mixture is soaked for 1 hour, and then ultrasonic-assisted extraction is performed for 30 minutes at a temperature of 40° C. After the extraction, the mixture is filtered to obtain a first filtrate and a first filter residue; 8 times the amount of 80 wt % ethanol solution is added to the first filter residue, the mixture is refluxed for 1.5 hours, and the second filtrate and the second filter residue are obtained by filtering; 6 times the amount of 80 wt % ethanol solution is added to the second filter residue, the mixture is refluxed for 1 hour, and the third filtrate is obtained by filtering.

[0043] (2) The filtrates were combined and concentrated under reduced pressure, then extracted and decolorized with an equal volume of ethyl acetate, and the aqueous phase after extraction was extracted with an equal volume of n-butanol; the n-butanol phase was then rotary evaporated to obtain a thick paste, and 800 g of white vaseline and 100 g of lanolin were added to every 100 g of the thick paste, and the mixture was heated and mixed to obtain an ointment.

[0044] Example 2

[0045] A Chinese herbal medicine composition for repairing skin comprises the following raw materials in parts by weight: 12 parts of angelica dahurica, 15 parts of white aconite root, 36 parts of mung bean, 24 parts of pulsatilla, 9 parts of gentian, 11 parts of dittany, 16 parts of andrographis paniculata, 18 parts of corydalis, 20 parts of cat's claw, 24 parts of lobelia, 10 parts of reed root and 22 parts of witch hazel.

[0046] The above-mentioned Chinese herbal medicine composition capable of repairing skin is made into an ointment and applied to prevent and / or treat acne. The preparation method of the ointment comprises the following steps:

[0047] (1) According to the weight proportion of the formula, Angelica dahurica, Radix Aconiti Lateralis, Mung Bean, Pulsatilla scabra, Gentiana scabra, Cortex Dictamni, Andrographis paniculata, Corydalis yanhusuo, Herba Cat's Claw, Herba Lobeliae, Rhizoma Phragmites, and Hamamelis Virginiana are weighed, the mixture is crushed into 80 meshes, 12 times the amount of 80 wt % ethanol solution of raspberry is added, the mixture is soaked for 1.5 h, and then ultrasonic-assisted extraction is performed for 45 min at a temperature of 45° C. After the extraction, the mixture is filtered to obtain a first filtrate and a first filter residue; 10 times the amount of 80 wt % ethanol solution is added to the first filter residue, the mixture is refluxed for 2 h, and the second filtrate and the second filter residue are obtained by filtering; 8 times the amount of 80 wt % ethanol solution is added to the second filter residue, the mixture is refluxed for 1.5 h, and the third filtrate is obtained by filtering.

[0048] (2) The filtrates were combined and concentrated under reduced pressure, then extracted and decolorized with an equal volume of ethyl acetate, and the aqueous phase after extraction was extracted with an equal volume of n-butanol; the n-butanol phase was then rotary evaporated to obtain a thick paste, and 800 g of white vaseline and 100 g of lanolin were added to every 100 g of the thick paste, and the mixture was heated and mixed to obtain an ointment.

[0049] Example 3

[0050] A Chinese herbal medicine composition for repairing skin comprises the following raw materials in parts by weight: 10 parts of angelica dahurica, 12 parts of white aconite root, 30 parts of mung bean, 20 parts of pulsatilla, 8 parts of gentian, 9 parts of dittany, 12 parts of andrographis paniculata, 15 parts of corydalis, 16 parts of cat's claw, 20 parts of lobelia, 8 parts of reed root, and 18 parts of witch hazel.

[0051] The above-mentioned Chinese herbal medicine composition capable of repairing skin is made into an ointment and applied to prevent and / or treat acne. The preparation method of the ointment comprises the following steps:

[0052] (1) According to the weight proportion of the formula, Angelica dahurica, Radix Aconiti Lateralis, Mung Bean, Pulsatilla scabra, Gentiana scabra, Cortex Dictamni, Andrographis paniculata, Corydalis yanhusuo, Herba Cat's Claw, Herba Lobeliae, Rhizoma Phragmites, and Hamamelis Virginiana are weighed, the mixture is crushed to 100 mesh, 15 times the amount of 80 wt % ethanol solution of Raspberry is added, the mixture is soaked for 2 h, and then ultrasonic-assisted extraction is performed for 60 min at a temperature of 50° C. After the extraction, the mixture is filtered to obtain a first filtrate and a first filter residue; 12 times the amount of 80 wt % ethanol solution is added to the first filter residue, the mixture is refluxed for 2.5 h, and the second filtrate and the second filter residue are obtained by filtering; 9 times the amount of 80 wt % ethanol solution is added to the second filter residue, the mixture is refluxed for 2 h, and the third filtrate is obtained by filtering.

[0053] (2) The filtrates were combined and concentrated under reduced pressure, then extracted and decolorized with an equal volume of ethyl acetate, and the aqueous phase after extraction was extracted with an equal volume of n-butanol; the n-butanol phase was then rotary evaporated to obtain a thick paste, and 800 g of white vaseline and 100 g of lanolin were added to every 100 g of the thick paste, and the mixture was heated and mixed to obtain an ointment.

[0054] Comparative Example 1

[0055] A Chinese herbal medicine composition for repairing skin, which is different from Example 3 in that gentian, corydalis, cat's claw and lobelia are replaced with other components in proportion, and the total mass remains unchanged. Then, the desired ointment is prepared according to the same process flow.

[0056] Comparative Example 2

[0057] A Chinese herbal medicine composition for repairing skin, which differs from Example 3 in that: Gentiana is replaced by Corydalis yanhusuo, cat's claw and Lobelia chinensis in proportion, and the total mass remains unchanged. Then, the desired ointment is prepared according to the same process flow.

[0058] Comparative Example 3

[0059] A Chinese herbal medicine composition for repairing skin, which differs from Example 3 in that: Corydalis yanhusuo is replaced by Gentiana scabra, Cat's Claw and Lobelia chinensis in proportion, and the total mass remains unchanged. Then, the desired ointment is prepared according to the same process flow.

[0060] Comparative Example 4

[0061] A Chinese herbal medicine composition for repairing skin, which differs from Example 3 in that cat's claw is replaced by three components, namely, Corydalis yanhusuo, Gentiana scabra and Lobelia chinensis, in proportion, and the total mass remains unchanged. Then, the desired ointment is prepared according to the same process flow.

[0062] Comparative Example 5

[0063] A Chinese herbal medicine composition for repairing skin, which differs from Example 3 in that: Lobelia is replaced with Corydalis yanhusuo, cat's claw and gentian in proportion, and the total mass remains unchanged. Then, the desired ointment is prepared according to the same process flow.

[0064] Comparative Example 6

[0065] A Chinese herbal medicine composition for repairing skin, which differs from Example 3 in that gentian, corydalis, cat's claw and lobelia are replaced with coptis chinensis of equal mass, and the total mass remains unchanged. Then, the desired ointment is prepared according to the same process flow.

[0066] Comparative Example 7

[0067] A Chinese herbal medicine composition for repairing skin, which differs from Example 3 in that the gentian is replaced with coptis chinensis of equal mass, and the total mass remains unchanged. Then, the desired ointment is prepared according to the same process flow.

[0068] Comparative Example 8

[0069] A Chinese herbal medicine composition for repairing skin, which differs from Example 3 in that: Corydalis yanhusuo is replaced with coptis chinensis of equal mass, and the total mass remains unchanged. Then, the desired ointment is prepared according to the same process flow.

[0070] Comparative Example 9

[0071] A Chinese herbal medicine composition for repairing skin, which differs from Example 3 in that cat's claw is replaced with coptis chinensis of equal mass, and the total mass remains unchanged. Then, the desired ointment is prepared according to the same process flow.

[0072] Comparative Example 10

[0073] A Chinese herbal medicine composition for repairing skin, which differs from Example 3 in that the lobelia is replaced with coptis chinensis of equal mass, and the total mass remains unchanged. Then, the desired ointment is prepared according to the same process flow.

[0074] Comparative Example 11

[0075] A Chinese herbal medicine composition for repairing skin, the formula of the Chinese herbal medicine composition adopts patent number CN202310643014.4, and the specific preparation method is: weigh 10g of Senecio radix, 10g of Gentiana scabra, 30g of Goat Milk, 30g of Bletilla striata, 30g of Bletilla striata, 10g of Centella asiatica, 30g of Poria cocos, 30g of Coix seeds, 30g of Mother of Pearl, and 15g of Imperata root, mix the above Chinese medicines, add 1500mL of pure water, soak for 30min, boil over high heat and then simmer for 30min, pour the decocted medicinal liquid into a container, add 1200mL of pure water to the residue, boil over high heat and then simmer for 30min, combine the two decocted medicinal liquids, pass through a 160-mesh sieve, centrifuge at 3000 rpm for 5min, take the supernatant, and concentrate it to make a Chinese medicine extract with a concentration of 0.33g / mL, and store it in a refrigerator at 4°C for standby use to obtain the Chinese medicine composition.

[0076] Minimum inhibitory concentration test of Propionibacterium acnes

[0077] Experimental reagents: anaerobic liquid culture medium, anaerobic agar, Japan Mitsubishi MGC anaerobic gas production bag, Propionibacterium acnes ATCC 6919.

[0078] The thick pastes of the Chinese herbal medicine compositions prepared in Examples 1-3 and Comparative Examples 1-10 were formulated into aqueous solutions with mass fractions of 10%, 5%, 2.5%, 1.25%, 0.625%, 0.25%, and 0.125% (v / v) for later use.

[0079] Experimental method: The strain was revived on anaerobic agar, cultured anaerobically at 37°C for 48 h, inoculated on anaerobic agar again, cultured anaerobically at 37°C for 48 h, and then prepared in anaerobic liquid culture medium (pH = 5.64) with a concentration of 10 5 CFU. Take a 96-well microplate and add 150 μL of samples to be screened at different concentrations of 10%, 5%, 2.5%, 1.25%, 0.625%, 0.25%, and 0.125% and 150 μL of anaerobic liquid culture medium with bacteria. Add 300 μL of anaerobic liquid culture medium with bacteria to the positive control group and 300 μL of sterile anaerobic liquid culture medium to the blank control group. Make 3 parallels for each dilution. After anaerobic culture at 37°C for 48 hours, observe the growth and take the positive control for good growth as the standard. The lowest sample concentration with no colony growth is taken as the minimum inhibitory concentration (MIC). The test results are shown in Table 1.

[0080] Table 1

[0081]

[0082]

[0083] The massive reproduction of Propionibacterium acnes stimulates the skin to produce an inflammatory response, forming typical acne symptoms such as papules and pustules. The experimental results show that the Chinese herbal extracts prepared in Examples 1-3 can have a significant inhibitory effect on Propionibacterium acnes at low concentrations.

[0084] Comparing Example 3 with Comparative Examples 1 to 5, it can be seen that the four natural plant active ingredients of Gentiana, Corydalis, Cat's Claw, and Lobelia produce a synergistic antibacterial effect after compounding, and have an excellent inhibitory effect on Propionibacterium acnes. The lack of any one of the components will significantly reduce the antibacterial effect. The inventor conducted in-depth research and found that when any two of the components are replaced by the remaining two components in proportion (the total mass remains unchanged), the minimum inhibitory concentration is further improved.

[0085] Comparing Example 3 with Comparative Example 6, it can be seen that the active ingredient of Coptis chinensis has a certain inhibitory effect on Propionibacterium acnes, but the effect is not as good as the product after the combination of Gentiana, Corydalis, Cat's Claw, and Lobelia. Comparing Example 3 with Comparative Examples 7 to 10, it can be seen that the combination of Coptis chinensis with any three components of Gentiana, Corydalis, Cat's Claw, and Lobelia does not have a synergistic antibacterial effect or the synergistic antibacterial effect is relatively weak.

[0086] Animal testing

[0087] First, the ointments prepared in Examples 1 to 3 were applied to the skin of mice. The results showed that no erythema, allergy, edema, etc. appeared on the skin of the experimental mice, indicating that the Chinese herbal ointment prepared by the present invention is highly safe, mild to the skin and non-irritating.

[0088] Efficacy test on P. acnes-induced ear inflammation in mice

[0089] Experimental method: (1) A strain of P. acnes was selected and cultured in brain heart infusion medium under anaerobic conditions at 37°C for 72 hours. The cultured bacteria were activated and proliferated. The bacterial colonies were collected, washed and filtered, and the concentration of the P. acnes culture solution was adjusted to 1×10 8 CFU / 20μl.

[0090] (2) Healthy mice were randomly divided into a control group, a model group, and groups 1 to 3 of Examples of the present invention.

[0091] 10. Mice were anesthetized intraperitoneally with pentobarbital (45 mg / kg), and the control group was intradermally injected with 20 μL of PBS solution in the center of the bilateral inner auricles of the mice, and the model group and the groups of Examples 1-3 of the present invention were intradermally injected with 20 μL of the above-mentioned P. acnes suspension in the center of the bilateral inner auricles of the mice. Five days after modeling, the groups of Examples 1-3 applied the Chinese herbal composition prepared in Examples 1-3 (all with a concentration of 0.33 g / mL) to the auricles of the mice, 0.1 mL each time, and the control group and the model group applied an equal amount of normal saline to the auricles of the mice, twice a day, for 14 consecutive days, and the mice were killed 24 hours after the last administration, and the mouse serum was collected. The ELISA method was used to perform the corresponding operations according to the instructions of the kit to detect the levels of IL-1β and TNF-ɑ. The test results are shown in Table 2.

[0092] Table 2

[0093] Group IL-1β / (ng / L) TNF-ɑ / (ng / L) Control group 41.59±6.01 16.84±2.57 Model Group 152.38±13.65 62.74±4.92 Example 1 72.46±7.06 37.35±3.60 Example 2 64.91±7.52 33.29±2.83 Example 3 51.06±5.64 22.37±2.18

[0094] As shown in Table 2, compared with the control group, the IL-1β and TNF-ɑ levels in the serum of the model group mice were significantly increased, confirming that the P.acnes intradermal injection mouse ear inflammation model was successfully established. Compared with the model group mice, the Chinese herbal medicine compositions prepared in Examples 1-3 of the present invention can reduce the IL-1β and TNF-ɑ levels in the serum of mice, and the difference is statistically significant (P<0.05), indicating that the Chinese herbal medicine composition of the present invention has the efficacy of treating acne.

[0095] Clinical trials

[0096] Test subjects: 60 patients aged 15 to 30 years old, half of whom were male and half were female. All cases met the diagnostic criteria for "acne" (polymorphic lesions such as comedones, folliculitis papules, pustules and nodules, but no obvious skin ulcers). The above patients were randomly divided into 3 groups, 60 cases in each group, with a balanced distribution of males and females: Group 1 was treated with the ointment of Example 1 of the present application, Group 2 was treated with the ointment of Example 2 of the present application, and Group 3 was treated with the ointment of Example 3 of the present application.

[0097] Efficacy criteria: (1) Cured: The area of ​​facial acne is reduced by ≥90%, and the facial skin is shiny. (2) Significantly effective: The area of ​​facial acne is reduced by ≥60%, and the color is light red. (3) Effective: The area of ​​facial acne is reduced by ≥30%, and the color is bright red. (4) Ineffective: The area of ​​facial acne is reduced by <30%, and the color change is not obvious.

[0098] Treatment method: clean the face with warm water, and then apply the ointment prepared in the example to the skin lesions. All patients were treated once in the morning and evening for 8 consecutive weeks. The test results are shown in Table 3.

[0099] Table 3

[0100] Group Cured / person Significant effect / person Effective / person Invalid / person Total effective rate / % Example 1 12 4 3 1 95 Example 2 12 5 2 1 95 Example 3 14 5 1 0 100

[0101] The above records are only embodiments of the present invention. Any modification or change made by a person familiar with the present technology using the present invention shall fall within the patent scope claimed by the present invention and shall not be limited to those disclosed in the embodiments.

Claims

1. A Chinese herbal medicine composition capable of repairing skin, characterized in that: The invention comprises the following raw materials in parts by weight: 8-15 parts of angelica dahurica, 8-15 parts of white aconite root, 20-40 parts of mung bean, 12-24 parts of pulsatilla, 5-10 parts of gentian, 6-12 parts of dittany, 9-17 parts of andrographis paniculata, 8-18 parts of corydalis, 10-20 parts of cat's claw, 15-25 parts of lobelia, 3-10 parts of reed root and 12-24 parts of witch hazel.

2. A Chinese herbal medicine composition for repairing skin according to claim 1, characterized in that: The invention comprises the following raw materials in parts by weight: 8-12 parts of angelica dahurica, 10-15 parts of white aconite root, 24-36 parts of mung bean, 14-24 parts of pulsatilla, 6-9 parts of gentian, 7-11 parts of dittany, 10-16 parts of andrographis paniculata, 10-18 parts of corydalis, 12-20 parts of cat's claw, 16-24 parts of lobelia, 6-10 parts of reed root and 14-22 parts of witch hazel.

3. A Chinese herbal medicine composition capable of repairing skin according to claim 2, characterized in that: The invention comprises the following raw materials in parts by weight: 10 parts of angelica dahurica, 12 parts of white aconite root, 30 parts of mung bean, 20 parts of pulsatilla scabra, 8 parts of gentian, 9 parts of dittany, 12 parts of andrographis paniculata, 15 parts of corydalis, 16 parts of cat's claw, 20 parts of lobelia, 8 parts of reed root and 18 parts of witch hazel.

4. A method for preparing the Chinese herbal medicine composition for repairing skin according to any one of claims 1 to 3, characterized in that: The following steps are involved: S1. Weigh angelica dahurica, white aconite root, mung bean, pulsatilla, gentian, Dictamni cortex, andrographis paniculata, Corydalis yanhusuo, cat's claw, lobelia, reed root, and Hamamelis virginiana according to the weight of the formula, mix and crush to 60-100 mesh, add 80wt% ethanol solution of 10-15 times of raspberry, soak for 1-2h, then perform ultrasonic assisted extraction for 30-60min, the ultrasonic extraction temperature is 40-50℃, filter after the extraction to obtain a first filtrate and a first filter residue; add 80wt% ethanol solution to the first filter residue for reflux extraction, and filter to obtain a second filtrate and a second filter residue; S2. Combine the filtrates and concentrate under reduced pressure, then extract and decolorize with an equal volume of ethyl acetate, and then extract the aqueous phase with an equal volume of n-butanol; then rotary evaporate the n-butanol phase and concentrate to obtain the product.

5. The method for preparing a Chinese herbal medicine composition capable of repairing skin according to claim 4, characterized in that: The solid-liquid ratio of the first filter residue to the ethanol solution is 1:8-12, and the extraction time is 1.5-2.5 hours.

6. The method for preparing the skin-repairing Chinese herbal medicine composition according to claim 4, characterized in that: Add ethanol solution to the second filter residue, further reflux extraction, and filter to obtain a third filtrate.

7. The method for preparing the skin-repairing Chinese herbal medicine composition according to claim 6, characterized in that: The solid-liquid ratio of the second filter residue to the ethanol solution is 1:6-9, and the extraction time is 1-2 hours.

8. Use of the skin-repairing Chinese herbal medicine composition according to any one of claims 1 to 3 in the preparation of medicines or cosmetics for preventing and / or treating acne.

9. The use according to claim 8, characterized in that: The medicine or cosmetic includes powder, ointment, gel, spray or ointment.

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