An apparatus and method for microbial remediation of groundwater
By rotating an ultra-fine bubble generator and an integrated device for uniform distribution of bubbles and bacterial solution, the effective range of the bacterial solution is expanded, solving the problems of uneven bacterial agent dispersion and limited aeration range. This achieves full mixing of bacterial solution and oxygen and a long-term aerobic state, thereby improving the microbial remediation effect of groundwater.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- 浙江省环境科技股份有限公司
- Filing Date
- 2025-03-26
- Publication Date
- 2026-06-30
AI Technical Summary
Existing groundwater microbial remediation technologies suffer from problems such as uneven dispersion of microbial agents, limited aeration range, and uneven mixing of oxygen and microbial agents, resulting in poor remediation effects.
The device employs an integrated unit of an ultra-fine bubble generator and a bubble and bacterial solution distribution system. The asymmetrical force emitted from the aeration head causes the device to rotate, expanding the range of action of the bacterial solution. The ultra-fine bubbles create a long-lasting aerobic environment, ensuring that the bacterial solution is evenly dispersed and fully mixed.
It significantly improved the dispersion uniformity and range of action of the bacterial agent, stabilized the aerobic state, enhanced the mixing effect of the bacterial agent and oxygen, formed a highly efficient aerobic microbial remediation microenvironment, and improved the remediation effect of groundwater.
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Figure CN120097531B_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of groundwater pollution remediation technology, specifically to an apparatus and method for microbial remediation of groundwater. Background Technology
[0002] In the field of environmental engineering, microbial remediation technology is an important remediation method, especially in the treatment of groundwater pollution. This technology utilizes the metabolic activity of microorganisms to degrade and transform pollutants in groundwater, thereby achieving the purpose of remediation.
[0003] In the field of water treatment technology, aeration is a common technique. By injecting air into the water, the concentration of oxygen in the water can be increased, promoting the growth and reproduction of aerobic microorganisms, thereby improving the self-purification capacity of the water body.
[0004] Existing groundwater microbial remediation technologies mainly employ static addition of microbial agents, which involves directly adding the agents to the groundwater and relying on natural diffusion and mixing to achieve contact between the agents and pollutants. Aerobic microbial remediation technology, on the other hand, uses aeration devices to inject air into the groundwater, promoting the growth and reproduction of aerobic microorganisms.
[0005] However, existing groundwater microbial remediation technologies face several challenges in practical applications. First, the static addition of microbial agents limits their effective range, making it difficult to ensure uniform dispersion and leading to excessively high or low concentrations in localized areas, thus affecting remediation efficacy. Second, existing aeration devices struggle to adapt to complex underground environments, with limited aeration range and short gas retention times, making it difficult to maintain a consistently aerobic environment and achieve comprehensive groundwater remediation. Furthermore, current technologies often neglect the uniform mixing of air and microbial agents when injecting air into groundwater, resulting in a mismatch between oxygen and microbial agents in terms of effective area and duration, impacting microbial growth and remediation effectiveness.
[0006] Patent specification CN113290033A discloses a method for in-situ enhanced remediation of soil and groundwater using a microbubble generator. The method includes: Step 1, adding water and / or reagent to a dissolving tank and turning on a stirrer to prepare the remediation reagent; Step 2, using the microbubble generator to simultaneously draw in gas and the prepared reagent, and pressurizing it in a pump to produce microbubbles; Step 3, injecting the microbubble dissolved reagent into the contaminated soil and groundwater environment through pipelines via a group of injection wells; Step 4, after the microbubbles from Step 3 enter the ground, allowing them to slowly release and gradually rise; Step 5, repeating Step 3 until all designed injection points have been injected. In Step 1, the prepared remediation reagent, depending on the different pollutants in the soil and groundwater, includes any one or more combinations of oxidants, reducing agents, activators, regulators, microbial nutrients, or microbial strains. Although this patented technology introduces microbubbles, it is essentially still a static method of adding bacterial agents through natural diffusion, where the diffusion effect of microbubbles on bacterial agents is still very limited. Summary of the Invention
[0007] In view of the above-mentioned technical problems and the shortcomings existing in this field, the present invention provides an apparatus and method for microbial remediation of groundwater.
[0008] [1] A device for microbial remediation of groundwater, comprising an ultrafine bubble generator, a bubble delivery pipe, a bacterial solution storage device, a bacterial solution delivery pipe, an adapter, and an integrated device for uniformly distributing bubbles and bacterial solution;
[0009] The integrated device for uniformly distributing bubbles and bacterial solution includes a gas zone and a bacterial solution zone that are not interconnected. The gas zone is connected to a bubble delivery pipe, and the bacterial solution zone is connected to a bacterial solution delivery pipe. The integrated device for uniformly distributing bubbles and bacterial solution has a cylindrical structure. Aeration heads are evenly distributed on the side corresponding to the gas zone, and bacterial solution delivery ports are evenly distributed on the side corresponding to the bacterial solution zone. The air outlet direction of the aeration heads is perpendicular to the diameter direction of the cylinder. When the aeration heads spray gas, the integrated device for uniformly distributing bubbles and bacterial solution can rotate.
[0010] The ultrafine bubble generator is connected to the adapter via a bubble delivery pipe, and the bacterial solution storage device is also connected to the adapter via a bacterial solution delivery pipe. Below the adapter is an integrated device for uniformly distributing both the bubble and bacterial solution. The adapter connects the bubble delivery pipe and the bacterial solution delivery pipe above it, and the integrated device for uniformly distributing both the bubble and bacterial solution below it, providing support and fixation.
[0011] The air outlet direction of the aeration head of the present invention is perpendicular to the diameter direction of the cylinder of the integrated device for uniformly distributing bubbles and bacterial liquid. This layout can generate asymmetrical force during aeration, causing the integrated device for uniformly distributing bubbles and bacterial liquid to rotate clockwise or counterclockwise.
[0012] The device for groundwater microbial remediation of this invention is based on the asymmetric force generated when ultrafine bubbles are ejected from the aeration head. This causes the integrated device, which distributes both bubbles and bacterial solution evenly, to rotate, significantly expanding the range of action of the bacterial solution in the groundwater and ensuring uniform dispersion. At the same time, the ultrafine bubbles create a long-lasting and fully distributed aerobic environment, allowing the bacterial solution and ultrafine bubbles to mix thoroughly. This achieves a good match in terms of both the range and duration of action, forming a highly efficient aerobic microbial remediation microenvironment, which is beneficial for the microbial remediation of groundwater.
[0013] The aforementioned device for groundwater microbial remediation, including an ultrafine bubble generator and a bacterial solution storage device, can be installed on the ground in the area where groundwater needs to be remediated.
[0014] The device for microbial remediation of groundwater, wherein the ultrafine bubble generator can be made of metal and / or corrosion-resistant materials.
[0015] The aforementioned device for groundwater microbial remediation uses an ultrafine bubble generator that produces bubbles with a diameter below 1000 nm, an internal pressure of 3–10 bar, a float time of over 21 days at 1 meter, a retention time of over 90 days, and a bubble density exceeding 10. 8 per mL.
[0016] The device for microbial remediation of groundwater, which integrates air bubbles and bacterial solution, can be made of plastic composite materials and / or corrosion-resistant materials.
[0017] The device for microbial remediation of groundwater, wherein the height-to-diameter ratio of the integrated device for uniformly distributing air bubbles and bacterial solution can be 1 to 4:1, for example 2.5:1.
[0018] In some embodiments, the cylindrical side of the device for microbial remediation of groundwater is divided into two parts corresponding to the gas zone and the bacterial solution zone, respectively, in an integrated device for uniformly distributing bubbles and bacterial solution. The aeration head occupies half of the area of the cylindrical side, and the bacterial solution delivery port occupies half of the area of the cylindrical side.
[0019] The device for microbial remediation of groundwater has an aeration hole diameter of 1–3 mm.
[0020] The device for microbial remediation of groundwater has a bacterial solution delivery port diameter of 1–5 mm, such as 2 mm.
[0021] The aforementioned device for groundwater microbial remediation includes a microbial degradation agent stored in a bacterial solution storage device. The microbial degradation agent can be constructed by selecting highly complementary degradation strains to form a microbial degradation community, and the microbial degradation solution is prepared through large-scale cultivation and engineering parameter design. Furthermore, the microbial degradation agent may include *Variovorax paradoxus* and *Pseudomonas veronii*, among others.
[0022] [2] A method for microbial remediation of groundwater, using the device for microbial remediation of groundwater described in [1];
[0023] The method for microbial remediation of groundwater includes:
[0024] Start the ultra-fine bubble generator and deliver ultra-fine bubbles to the gas zone through the bubble delivery pipe. The bubbles are ejected through the aeration head. The asymmetric force generated when the ultra-fine bubbles are ejected causes the bubbles and bacterial liquid to be evenly distributed in the integrated device to rotate.
[0025] The bacterial solution storage device is activated, and the microbial solution is transported to the bacterial solution area through the bacterial solution delivery pipe. During the rotation of the integrated device for uniform distribution of bubbles and bacterial solution, the microbial solution is evenly fed into the groundwater from the bacterial solution delivery port.
[0026] This invention effectively increases the range of action of the bacterial solution in groundwater by utilizing the rotation caused by ultra-fine bubble aeration and ensures uniform dispersion of the bacterial solution. At the same time, it uses ultra-fine bubbles to create a fully distributed and long-lasting aerobic environment, which allows the bacterial solution and ultra-fine bubbles to mix thoroughly, forming a good aerobic microbial remediation microenvironment. This achieves a good match between the range of action and the time of action, thereby improving the microbial remediation effect of groundwater.
[0027] Compared with the prior art, the beneficial effects of this invention are as follows:
[0028] 1. More uniform dispersion and wider range of action: Existing technologies use static addition of bacterial agents, relying on natural diffusion and mixing, resulting in uneven dispersion and limited range of action. This invention, however, utilizes the asymmetric force generated when ultra-fine bubbles are ejected from the aeration head, causing the integrated device for uniformly distributing bubbles and bacterial solution to rotate. This significantly expands the range of action of the bacterial solution in groundwater and ensures uniform dispersion, avoiding the problem of excessively high or low concentrations of bacterial agent in localized areas, thus improving the remediation effect.
[0029] 2. More stable aerobic state and better aeration effect: Existing aerobic microbial remediation technologies use aeration devices that are difficult to adapt to complex underground environments, have limited aeration range, short gas retention time, and are difficult to maintain an aerobic state for a long time. This invention utilizes ultrafine bubbles to create a long-lasting and fully evenly distributed aerobic state, solving the shortcomings of existing aeration devices. This is conducive to the growth and reproduction of aerobic microorganisms, thereby improving the self-purification capacity of water bodies and achieving comprehensive remediation of groundwater.
[0030] 3. More thorough mixing of microbial agents and air, resulting in more precise matching of effects: Existing technologies often neglect the uniform mixing of air and microbial agents when injecting air into groundwater, leading to a mismatch between oxygen and the microbial agents in terms of the area and time of action. This invention achieves a good match in both the range and time of action by thoroughly mixing the bacterial solution with ultrafine bubbles, forming a highly efficient aerobic microbial remediation microenvironment. This is conducive to the growth of microorganisms and the exertion of their remediation effects, further enhancing the effectiveness of groundwater microbial remediation. Attached Figure Description
[0031] Figure 1 This is a schematic diagram of the device structure for microbial remediation of groundwater in a specific implementation.
[0032] Figure 2 This is a schematic diagram of the device transfer structure for groundwater microbial remediation in a specific implementation.
[0033] Figure 3 This is a schematic diagram of the cross-sectional structure of the integrated device for the microbial remediation of groundwater, in a specific embodiment. The arrows indicate the orientation of the aeration heads, which are perpendicular to the diameter of the cylinder. Detailed Implementation
[0034] The present invention will be further described below with reference to the accompanying drawings and specific embodiments. It should be understood that these embodiments are for illustrative purposes only and are not intended to limit the scope of the invention. Operating methods not specifically specified in the following embodiments are generally performed under conventional conditions or as recommended by the manufacturer.
[0035] See Figures 1 to 3A device for microbial remediation of groundwater includes an ultrafine bubble generator 1, a bubble delivery pipe 2, a bacterial solution storage device 3, a bacterial solution delivery pipe 4, an adapter 5, and an integrated device for uniformly distributing bubbles and bacterial solution 6. The integrated device 6 is divided into separate gas and bacterial solution zones, with the gas zone connected to the bubble delivery pipe 2 and the bacterial solution zone connected to the bacterial solution delivery pipe 4. The integrated device 6 has a cylindrical structure. The side of the cylinder is divided into two parts corresponding to the gas and bacterial solution zones, respectively. Aeration heads 7 are evenly distributed on the side corresponding to the gas zone, occupying half of the side area of the cylinder. Bacterial solution delivery ports 8 are evenly distributed on the side corresponding to the bacterial solution zone, also occupying half of the side area of the cylinder. The air outlet direction of the aeration heads 7 is perpendicular to the diameter direction of the cylinder, and the aeration heads 7 can cause the integrated device 6 to rotate when they spray gas. An ultrafine bubble generator 1 is connected to an adapter 5 via a bubble delivery pipe 2. A bacterial solution storage device 3 is connected to the adapter 5 via a bacterial solution delivery pipe 4. Below the adapter 5 is an integrated device 6 for uniformly distributing both the bubble and the bacterial solution. The ultrafine bubble generator 1 is made of metal and / or corrosion-resistant materials. The bubbles generated by the ultrafine bubble generator 1 have a diameter of less than 1000 nm, an internal pressure of 3–10 bar, a float time of over 21 days at 1 m, a retention time of over 90 days, and a bubble density exceeding 10. 8 aerobic microbial agents are stored in the bacterial solution storage device 3. The integrated device 6, which distributes both air bubbles and bacterial solution evenly, is made of plastic composite materials and / or corrosion-resistant materials.
[0036] A method for microbial remediation of groundwater, employing the aforementioned apparatus for microbial remediation of groundwater, comprising:
[0037] Step 1: Prepare the following components: 1. Ultrafine bubble generator; 2. Bubble delivery pipe; 3. Bacterial solution storage device; 4. Bacterial solution delivery pipe; 5. Adapter; and 6. Integrated device for uniform distribution of bubbles and bacterial solution. The ultrafine bubble generator 1 utilizes high-speed rotary cutting and Venturi mixing technology to produce ultrafine bubbles with a diameter of less than 1000 nm. Compared to ordinary bubbles, ultrafine bubbles possess unique physicochemical properties, exhibiting high density, long float time, and long retention time, thus homogenizing and enhancing dissolved oxygen. The ultrafine bubble generator 1 is made of metal and produces bubbles with a density exceeding 10... 8 The bacterial culture storage device 3 uses a 50L stainless steel tank to store the microbial degradation agent. The microbial degradation agent consists of Variovorax paradoxus and Pseudomonas veronii at a 1:1 bacterial culture volume ratio (bacterial culture OD). 600A bacterial solution composed of the same volume (when the concentration is 1.0) was used for the degradation of benzene series compounds in groundwater. The effective viable bacteria count of the bacterial solution used was ≥100 million / L, and the number of molds and other bacteria was ≤3.0 × 10⁻⁶. 6 The bacterial count / mL was ≤10%, and under laboratory evaluation conditions, the degradation rate of benzene series compounds reached over 50% within one month. The ultrafine bubble generator 1 and the bacterial solution storage device 3 were installed on the ground in the benzene series compound contaminated area of the groundwater in the medical and chemical industrial park.
[0038] Step 2: Connect the ultrafine bubble generator 1 to the adapter 5 via the bubble delivery pipe 2, and connect the bacterial solution storage device 3 to the adapter 5 via the bacterial solution delivery pipe 4. Connect the adapter 5 to the integrated bubble and bacterial solution distribution device 6 below. The integrated bubble and bacterial solution distribution device 6 is made of plastic composite material, and is a cylindrical structure with a diameter of 20cm and a height of 50cm. The side of the cylinder is divided into two parts: one part is evenly distributed with aeration heads 7, and the other part is evenly distributed with bacterial solution delivery ports 8. The aeration heads 7 have a hole diameter of 1mm and a spacing of 1cm, and the orientation of the aeration heads 7 is perpendicular to the diameter direction of the cylinder. The bacterial solution delivery ports 8 have a hole diameter of 2mm and are evenly distributed on the side of the cylinder.
[0039] Step 3: Start the ultrafine bubble generator 1, and supply ultrafine bubbles to the adapter 5 through the bubble delivery pipe 2. The ultrafine bubbles have a diameter of less than 1000 nm, an internal pressure of 3-10 bar, a floating time of more than 21 days at 1 meter, and a lifespan of more than 90 days. The ultrafine bubbles enter the integrated bubble and bacterial solution distribution device 6 through the adapter 5 and are ejected through the aeration head 7. Based on the asymmetric force generated when the ultrafine bubbles are ejected, the integrated bubble and bacterial solution distribution device 6 rotates itself.
[0040] Step 4: Simultaneously, activate the bacterial solution storage device 3, and deliver the microbial agent to the adapter 5 through the bacterial solution delivery pipe 4. The microbial agent enters the integrated bubble and bacterial solution distribution device 6 through the adapter 5, and is then delivered into the groundwater from the bacterial solution delivery port 8.
[0041] Step 5: By controlling the flow rates of the ultrafine bubble generator 1 and the bacterial solution storage device 3, the aeration rate and the amount of bacterial agent added are adjusted to ensure that the residence time of the ultrafine bubbles matches the remediation cycle of the microbial agent, and that the action area of the ultrafine bubbles matches the action range of the microbial agent, thus guaranteeing the microbial remediation effect of the groundwater. Oxygen supply is a limiting factor for the degradation of benzene series compounds in the groundwater of contaminated sites. Ultrafine bubbles have a high specific surface area and long residence time, which can significantly increase the dissolved oxygen concentration. Ordinary bubbles float 1 meter in water for less than ten minutes and exist for several hours. In contrast, ultrafine bubbles float 1 meter for more than 21 days and exist for more than 90 days, which can homogenize and increase dissolved oxygen. The results show that under the condition of increased dissolved oxygen concentration of 2-3 mg / L, the degradation rate of benzene series compounds by microorganisms is increased, with the biodegradation rate of benzene increasing by more than 60%. The remediation of contaminated groundwater at the site meets the standards within a 90-day remediation cycle.
[0042] Through the above steps, the integrated bubble and bacterial solution distribution device 6 of this embodiment can significantly expand the range of action of the bacterial solution, achieve uniform dispersion of the bacterial solution in groundwater, and improve the microbial remediation effect of groundwater through the long-term action of ultrafine bubbles and the full and uniform mixing of bubbles and bacterial solution.
[0043] Furthermore, it should be understood that after reading the above description of the present invention, those skilled in the art can make various alterations or modifications to the present invention, and these equivalent forms also fall within the scope defined by the appended claims.
Claims
1. A device for microbial remediation of groundwater, characterized in that, It includes an ultra-fine bubble generator (1), a bubble delivery pipe (2), a bacterial liquid storage device (3), a bacterial liquid delivery pipe (4), an adapter (5), and an integrated device for uniformly distributing bubbles and bacterial liquid (6). The integrated device for uniformly distributing bubbles and bacterial liquid (6) includes a gas zone and a bacterial liquid zone that are not interconnected. The gas zone is connected to the bubble delivery pipe (2), and the bacterial liquid zone is connected to the bacterial liquid delivery pipe (4). The integrated device for uniformly distributing bubbles and bacterial liquid (6) is a cylindrical structure. Aeration heads (7) are evenly distributed on the side corresponding to the gas zone, and bacterial liquid delivery ports (8) are evenly distributed on the side corresponding to the bacterial liquid zone. The cylindrical side of the integrated device for uniformly distributing bubbles and bacterial liquid (6) is divided into two parts corresponding to the gas zone and the bacterial liquid zone, respectively. The aeration heads (7) occupy half of the area of the cylindrical side, and the bacterial liquid delivery ports (8) occupy half of the area of the cylindrical side. The air outlet direction of the aeration heads (7) is perpendicular to the diameter direction of the cylinder. When the aeration heads (7) spray gas, the integrated device for uniformly distributing bubbles and bacterial liquid (6) can rotate. The ultra-fine bubble generator (1) is connected to the adapter (5) through the bubble delivery pipe (2), the bacterial liquid storage device (3) is connected to the adapter (5) through the bacterial liquid delivery pipe (4), and the adapter (5) is connected to the integrated device for uniform distribution of bubbles and bacterial liquid (6). The ultra-fine bubble generator (1) delivers ultra-fine bubbles to the gas zone through the bubble delivery pipe (2). The bubbles are ejected through the aeration head (7). The asymmetrical force generated when the ultra-fine bubbles are ejected causes the bubbles and bacterial liquid to be evenly distributed. The integrated device (6) rotates. The bacterial liquid storage device (3) delivers microbial liquid to the bacterial liquid area through the bacterial liquid delivery pipe (4). During the rotation of the integrated device (6) for uniform distribution of microbial liquid and air bubbles, the microbial liquid is evenly fed into the groundwater from the bacterial liquid delivery port (8).
2. The device for microbial remediation of groundwater according to claim 1, characterized in that, The ultrafine bubble generator (1) and the bacterial liquid storage device (3) are placed on the ground in the area where the groundwater needs to be remediated.
3. The device for microbial remediation of groundwater according to claim 1, characterized in that, The ultrafine bubble generator (1) is made of metal and / or corrosion-resistant materials; The ultrafine bubble generator (1) produces bubbles with a diameter of less than 1000 nm, an internal pressure of 3~10 bar, a float time of more than 21 days to 1 m, a residence time of more than 90 days, and a bubble density of more than 10. 8 per mL.
4. The apparatus for microbial remediation of groundwater according to claim 1, characterized in that, The integrated device for uniform distribution of bubbles and bacterial liquid (6) is made of plastic composite material and / or corrosion-resistant material; The ratio of the height to the diameter of the cylinder in the integrated device for uniform distribution of bubbles and bacterial liquid (6) is 1~4:
1.
5. The apparatus for microbial remediation of groundwater according to claim 1, characterized in that, The diameter of the aeration head (7) is 1~3 mm.
6. The apparatus for microbial remediation of groundwater according to claim 1, characterized in that, The diameter of the bacterial liquid delivery port (8) is 1~5 mm.
7. The apparatus for microbial remediation of groundwater according to claim 1, characterized in that, Microbial degradation agents are stored in the bacterial liquid storage device (3); The microbial degradation agents include *Gnaphalium affine* and *Pseudomonas vesiculosus*.
8. A method for microbial remediation of groundwater, characterized in that, The apparatus for microbial remediation of groundwater as described in any one of claims 1 to 7 is used; The method for microbial remediation of groundwater includes: Start the ultra-fine bubble generator (1), and deliver ultra-fine bubbles to the gas zone through the bubble delivery pipe (2). The bubbles are ejected through the aeration head (7). The asymmetrical force generated when the ultra-fine bubbles are ejected causes the bubbles and bacterial liquid to be evenly distributed. The integrated device (6) rotates. Start the bacterial liquid storage device (3) and deliver the microbial liquid to the bacterial liquid area through the bacterial liquid delivery pipe (4). During the rotation of the integrated device (6) for uniform distribution of microbial liquid and air bubbles, the microbial liquid is evenly fed into the groundwater from the bacterial liquid delivery port (8).