Rhodiola rosea double-effect nano cold soap and preparation method thereof
By using a combination of alpine rhodiola and nanosilver in cold soap, a highly effective antibacterial dual-effect nano-cold soap was prepared, which solved the problem of insufficient antibacterial effect of existing soap liquids and achieved significant inhibition and killing effects on a variety of pathogens.
Patent Information
- Application Number
- CN202510116569.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-24
- Publication Date
- 2025-06-06
AI Technical Summary
The existing cold soap has shortcomings in its antibacterial effect, especially in the inhibition and killing ability of various pathogens.
The preparation method of alpine Rhodiola double-effect nano-cooled soap is prepared by mixing sodium hydroxide with nanosilver sol to form nanosilver alkali liquid, and mixing it with oil and alpine Rhodiola powder. The nano-anti-bacterial double-effect cold soap is prepared through specific process steps.
The efficient inhibition and killing of Staphylococcus aureus and Candida albicans was achieved, especially under specific conditions, the bactericidal rate of Staphylococcus aureus reached 95%, and the bactericidal rate of Candida albicans reached 92%.
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Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of nano antibacterial and daily necessities, and particularly relates to alpine rhodiola rosea double-effect nano cold-process soap and a preparation method thereof. Background Art
[0002] Rhodiola rosea (Rhodiola sachalinensis), also known as Sakhalin Rhodiola rosea, is a perennial herb with strong vitality. It has a significant effect in anti-hypoxia, anti-radiation, anti-fatigue, anti-virus and other resistance tests. The main components of Rhodiola rosea are salidroside and tyrosol, in addition to starch, protein, fat, tannin, flavonoids and trace volatile oils, as well as trace elements such as iron, zinc, tin, molybdenum, and manganese. It is mainly used to treat sexual dysfunction, diabetes, hypotension and other diseases.
[0003] Cold-process soap uses natural plant oils and fats without unnecessary chemical additives (flavors, preservatives, pigments, chemically synthesized surfactants, etc.); during the saponification process, cold-process soap will produce about 20% natural glycerin, which is a very good skin moisturizer that can maximize the retention of skin care ingredients in the oil. The oil also contains nutrients that do not participate in saponification.
[0004] It can maintain the skin while cleansing it. Skin conditioning ingredients such as plant essence, various essential oils, goat milk, sea salt, propolis, etc. can also be added to cold-processed handmade soap to enhance the effect of handmade soap. Summary of the invention
[0005] The invention aims to provide a nano antibacterial alpine rhodiola rosea double-effect nano cold-process soap and a preparation method thereof.
[0006] The preparation method of alpine rhodiola rosea double-effect nano cold-process soap comprises:
[0007] 1) Pour sodium hydroxide into a container containing nanosilver sol and cool it to 40°C to obtain nanosilver alkali solution;
[0008] The weight ratio of sodium hydroxide to nano silver sol is 0.8-1.2:2.5;
[0009] 2) Heat the oil to 60°C and then cool it to 40°C;
[0010] 3) Pour 350-400g of nanosilver lye into 1000g of oil, stirring while adding;
[0011] 4) Add 0.3-0.5g of alpine rhodiola rosea powder to every 1000g of oil;
[0012] 5) Pour the soap solution into the mold gently and keep it warm for 24 hours before demoulding;
[0013] 6) Place the demoulded soap in a cool and dry place and let it mature for 3-4 weeks before use;
[0014] The oil comprises 25%-35% white oil, 25%-35% coconut oil, 15%-25% castor oil and 15%-25% canola oil in weight percentage, sodium hydroxide, nano silver sol and rhodiola rosea powder.
[0015] The weight ratio of sodium hydroxide to nano silver sol in step 1) is 1:2.5;
[0016] Step 4) adding 0.4 g of alpine rhodiola rosea powder to every 1000 g of oil;
[0017] The rhodiola rosea powder is prepared by the following method:
[0018] 1) Weigh 20 g of dried roots of Rhodiola rosea, place them in an appropriate container, add 100 times the amount of distilled water and soak for 5 days; after soaking, filter with non-woven fabric and collect the filtrate and residue separately.
[0019] 2) Place the filter residue in a stainless steel pot, add 30 times the amount of distilled water and boil for 20 minutes; after boiling, filter with non-woven fabric to collect the alpine Rhodiola rosea filtrate;
[0020] 3) Mix the distilled water soaking filtrate and the decoction filtrate, and concentrate them using a rotary evaporator. Stop the operation when the volume of the concentrate reaches about 10 mL; freeze-dry to obtain the alpine Rhodiola rosea water extract, seal it in a brown reagent bottle, and place it in a 4°C refrigerator for later use;
[0021] 4) Take a certain volume of macroporous resin D101 in a large beaker, slowly add 95% volume concentration ethanol solution into the beaker until the ethanol solution level exceeds the macroporous resin particles, let it stand for about 12 hours, rinse with distilled water until there is no smell, and then load it into the column. Load it to two-thirds of the glass column, prepare the alpine Rhodiola rosea water extract to a concentration of 5 mg / mL for sample loading, and the sample volume is one-third of the volume of the macroporous resin. After the loading is completed, let it stand for 12 hours, rinse with distilled water, 10% ethanol, 20% ethanol, and 80% ethanol in turn, collect the 80% ethanol elution component, concentrate the eluate, and dry it into powder, which is the Rhodiola rosea powder.
[0022] The nano silver sol is prepared by the following method:
[0023] 1) Rinse the purple cabbage with distilled water, dry it, chop it, weigh 50-60 grams, add 100 mL of distilled water, heat and boil for 5 minutes, cool to room temperature, and vacuum filter to obtain the purple water extract;
[0024] 2) Dilute to 250 mL with distilled water and store at 4°C for later use;
[0025] 3) Mix the purple cabbage water extract and 0.001 mol∙L -1 Silver nitrate is mixed in a volume ratio of 1-9:9-1, placed under a xenon lamp light source under magnetic stirring, and reacted for 1-40 minutes.
[0026] Light intensity is 1W∙cm 2 , react for 1 to 20 minutes.
[0027] Step 3) The volume ratio is 8:2, and the reaction is carried out for 20 minutes.
[0028] The invention provides a preparation method of alpine rhodiola rosea double-effect nano cold-processed soap, comprising: 1) pouring sodium hydroxide into a container filled with nano silver sol, cooling to 40°C to obtain nano silver alkali solution; the weight ratio of the sodium hydroxide to the nano silver sol is 0.8-1.2:2.5; 2) heating fat to 60°C, and then cooling to 40°C; 3) pouring 350-400g of nano silver alkali solution into 1000g of fat, stirring while adding; 4) adding 0.3-0 .5g alpine rhodiola rosea powder; 5) pour the soap solution into the mold gently and demold it after keeping warm for 24 hours; 6) place the demolded handmade soap in a cool and dry place and let it stand for 3-4 weeks before use; the bactericidal rate of 2×MIC against Staphylococcus aureus reaches 95% at 3min, while it is only 92% for Candida albicans under the same conditions; the bactericidal rate of 2×MIC against Staphylococcus aureus reaches 92.86% at 30s, while it is 86.50% for Candida albicans under the same conditions. BRIEF DESCRIPTION OF THE DRAWINGS
[0029] Figure 1 UV-visible absorption spectra of synthesized nanosilver with different volume ratios of reaction solution;
[0030] Figure 2 UV-visible absorption spectra of silver nanoparticles at different reaction time intervals (every 5 min);
[0031] Figure 3 UV-visible absorption spectra of synthesized nanosilver under different light intensities;
[0032] Figure 4 Infrared spectrum of purple cabbage extract;
[0033] Figure 5 TEM electron microscope image of nanosilver;
[0034] Figure 6 XRD pattern of nanosilver;
[0035] Figure 7 The antibacterial effect of different soap samples on pathogenic bacteria;
[0036] Figure 8 Time killing curve of sample soap BAC against pathogens; A: Time killing curve of sample soap BAC against Staphylococcus aureus; B: Time killing curve of sample soap BAC against Candida albicans. DETAILED DESCRIPTION
[0037] Example 1 Preparation of Rhodiola rosea powder
[0038] Weigh 20g of dried roots of Rhodiola rosea, place them in an appropriate container, add 100 times the amount of distilled water and soak for 5 days. After soaking, filter with non-woven fabric and collect the filtrate and residue respectively. Place the residue in a stainless steel pot, add 30 times the amount of distilled water to the pot and boil for 20 minutes. After decoction, filter with non-woven fabric and collect the filtrate of Rhodiola rosea. Mix the distilled water soaking filtrate and the decoction filtrate, use a rotary evaporator to concentrate, and stop the operation when the volume of the concentrated solution reaches about 10mL. Freeze-dry to obtain the water extract of Rhodiola rosea, seal it in a brown reagent bottle, and place it in a 4°C refrigerator for use.
[0039] Take a certain volume of macroporous resin (D101) in a large beaker, slowly add 95% volume concentration ethanol solution into the beaker until the ethanol solution level exceeds the macroporous resin particles, let it stand for about 12 hours, rinse with distilled water until there is no smell, then load it into the column, load it to two-thirds of the glass column, prepare the alpine Rhodiola rosea water extract into a concentration of 5 mg / mL for loading, and the loading amount is one-third of the volume of the macroporous resin. After loading, let it stand for 12 hours, rinse with distilled water, 10% ethanol, 20% ethanol, and 80% ethanol in turn, collect the 80% ethanol elution component, concentrate the eluate, and dry it into powder, which is the Rhodiola rosea powder.
[0040] Example 2 Preparation of Nanosilver Sol
[0041] 1. Synthesis of Nanosilver
[0042] After the purple cabbage was rinsed with distilled water, it was dried and chopped. 50 g of the purple cabbage was weighed, and 100 mL of distilled water was added to boil for 5 min. The purple water extract was obtained by vacuum filtration, and then diluted to 250 mL with distilled water and stored at 4 °C for later use. -1 Silver nitrate and purple cabbage water extract were mixed in different volume ratios (1:9, 2:8, 3:7, 4:6, 5:5, 6:4, 7:3, 8:2, 9:1) and irradiated under a 350W xenon lamp light source for a certain period of time (0-40min) under magnetic stirring. When the reaction was completed, the mixed solution changed from light purple to reddish brown, and the product was verified by UV-visible absorption spectroscopy.
[0043] 2. Optimization of Nanosilver Preparation Conditions
[0044] 1) Effect of reactant volume ratio
[0045] The purple cabbage extract and 0.001 mol∙L -1 AgNO 3 The volume ratios were 9:1, 8:2, 7:3, 6:4, 5:5, 4:6, 3:7, 2:8 and 1:9, respectively, and the light intensity was 1 W∙cm 2 After 20 minutes of reaction, the UV-visible absorption spectrum of the solution was measured. Figure 1 As shown. In the wavelength range of 400-450nm, nanosilver will have a typical absorption peak. Figure 1 It can be observed that the surface plasmon resonance absorption peak of nanosilver is in the wavelength range of 407nm-437nm, which proves that nanosilver is generated under the above reaction conditions. When the volume ratio of purple cabbage to silver nitrate solution is 9:1, the absorption peak of the generated nanosilver is located at 407nm. When the reaction ratio of the two is gradually reduced to 1:9, the absorption peak of nanosilver gradually red-shifts from 407nm to 437nm, indicating that the particle size of nanosilver gradually increases. This may be due to the reduction in the proportion of purple cabbage water extract, and the generated nanosilver particles agglomerate close to the silver core, making the nanosilver particle size larger and the ultraviolet-visible absorption peak red-shifted. When the volume of purple cabbage water extract and silver nitrate is 8:2, the ultraviolet-visible spectrum absorbance value of nanosilver is the largest, at 414nm, and from Figure 1 It can also be seen from the color illustration that the color of the nanosilver solution is the darkest at this reaction ratio, indicating that the concentration of nanosilver in the solution is the highest. Therefore, the volume ratio of purple cabbage water extract to silver nitrate was determined to be 8:2 as the optimal reaction ratio for subsequent research.
[0046] 2) Impact of reaction time
[0047] After mixing the purple cabbage water extract and silver nitrate in a volume ratio of 8:2, the light intensity was 1W∙cm 2 2 mL of the reaction solution was taken out every 5 minutes for UV-visible spectrum scanning. The results are as follows Figure 2 As shown. 5 minutes after the reaction started, the characteristic absorption peak of nanosilver appeared at 414nm. As the reaction time increased, the absorbance value gradually increased. This is because the amount of nanosilver generated increased gradually with the extension of the reaction time. Figure 2 It can also be seen that the absorbance value of nanosilver increases rapidly when the reaction time is short from 5min to 20min. After 20min, the absorbance growth rate of nanosilver slows down, indicating that the reaction between purple cabbage water extract and silver nitrate is mainly concentrated in the 0-20min time period of illumination. Considering the time efficiency factor, the optimal reaction time is determined to be 20min
[0048] 3) Effect of light intensity on the reaction
[0049] The purple cabbage water extract was mixed with silver nitrate in a volume ratio of 8:2 and the samples were exposed to different light intensities (1.5 W∙cm 2 , 1W∙cm 2 , 500mW∙cm 2 After 20 minutes of reaction, the UV-visible scanning spectrum of the reaction solution was measured. The results are as follows Figure 3 When the light intensity is 500mW∙cm 2 When the light intensity is 1 W∙cm 2 When the light intensity increases to 1.5 W∙cm 2 When the absorbance of the nanosilver solution was 0.872, the increase was not large. Therefore, considering the light utilization rate, the optimal light intensity for the reaction was determined to be 1 W∙cm 2 .
[0050] 4) Calculation of Nanosilver Yield
[0051] The silver ion concentration before the reaction was 21.6 mg / L (0.001 mol / L×108×1000×2mL÷10 mL). After the reaction, the silver ion concentration measured by ICP spectroscopy was 0.009856 mg / L. The calculated nanosilver yield was 99.96%.
[0052] 3) Characterization of Nanosilver
[0053] 1) Infrared spectroscopy
[0054] The infrared spectrum of purple cabbage extract is as follows Figure 4 As shown, 3386cm -1 The stretching vibration of hydroxyl group is 2928cm -1 Methylene -CH 2 -Stretching vibration, 1626cm -1 Related to the stretching vibration of carbon-carbon double bond, 1412 cm -1 The methyl group has an angle-variable vibration, 1052 cm -1 The stretching vibration of CO bond and the skeleton vibration of CC single bond are at 800cm -1 Nearby is the out-of-plane bending vibration of the CH bond. These characteristic peaks confirm the presence of flavonoids and phenolic compounds in the purple cabbage water extract, which have reducing properties and can reduce the silver ions in silver nitrate to nanosilver.
[0055] 2) TEM images
[0056] From the transmission electron microscope image of nanosilver ( Figure 5) It can be seen that the nanosilver particles are spherical or ellipsoidal, some of the nanosilver particles are slightly agglomerated, the particle diameter is about 10nm, and the particle size is relatively uniform.
[0057] 3) X-ray diffraction analysis
[0058] From the XRD spectrum of nanosilver ( Figure 6 ) It can be seen that the 2θ angle is located at 38.17 ◦ , 44.23 ◦ , 64.57 ◦ , 77.27 ◦ and 81.37 ◦ There are five diffraction peaks, corresponding to the five crystal planes of nanosilver (111), (200), (220), (311), and (222). In the figure, there is a slight noise, which is due to the presence of a small amount of plant extract on the surface of nanosilver.
[0059] Example 3 Alpine Rhodiola rosea double-effect nano cold-process soap
[0060] 1. Handmade soap formula: white oil (25%-35%), coconut oil (25%-35%), castor oil (15%-25%), canola oil (15%-25%), sodium hydroxide, nano silver sol, rhodiola rosea powder.
[0061] 2. Soap making steps:
[0062] (1) Weigh a certain amount of sodium hydroxide and pour it into a measuring cup containing nanosilver sol (sodium hydroxide: nanosilver sol = 1:2.5), and cool it to 40℃.
[0063] (2) Weigh various oils and fats, place them directly into a stainless steel basin, heat them to 60°C and then cool them to 40°C.
[0064] (3) Slowly pour 378 grams of lye into 1000 grams of oil and start stirring.
[0065] (4) After stirring until a clear “8” can be drawn on the surface of the soap liquid, add 1000g of mixed oil and 0.4g of alpine rhodiola rosea powder, stir evenly and then pour into the mold.
[0066] (5) Pour the soap solution gently into the mold and remove it from the mold after keeping it warm for 24 hours.
[0067] (6) Place the demoulded soap in a cool, dry place and let it mature for 3-4 weeks before use.
[0068] Example 4 Detection of the antibacterial effect of different soap samples on pathogenic bacteria
[0069] 1. Preparation method of sample soap BAC soap solution
[0070] Take 20g of sample BAC and dissolve it in 100ml of distilled water in a 60℃ water bath. After dissolution, the sample concentration is 200mg / ml, which is the sample soap BAC soap solution.
[0071] 2. Experimental methods: Punch method and microbroth dilution method were used to determine the antibacterial effects of different sample soaps on Staphylococcus aureus and Candida albicans.
[0072] This experiment found that (see Figure 7 ), each group of samples had different degrees of antibacterial effect on Candida albicans and Staphylococcus aureus, among which the BAC group had the best antibacterial effect on Candida albicans, and the diameter of the antibacterial circle could reach 3cm; at the same dose, the overall antibacterial effect of BC was better than that of BA; from the results of the blank group, the soap solution itself had an antibacterial effect on Candida albicans and Staphylococcus aureus, but its antibacterial circle diameter was lower than that of other sample groups, indicating that the addition of A or C could improve the antibacterial ability of the soap solution. By comparing the BA group and the blank group, we found that the addition of sample A could improve the antibacterial effect of the sample soap on Candida albicans, indicating that sample A had specific killing ability on Candida albicans; by comparing the blank group and the BC group, we found that the addition of sample C could improve the antibacterial ability of the soap solution on Staphylococcus aureus and Candida albicans; by comparing the BAC group and the BA group, we found that the addition of sample C could help further improve the antibacterial ability of the soap solution on Candida albicans.
[0073] ;
[0074] By observing the turbidity in each treated sample well, the concentration at which no bacterial growth was observed was defined as the minimum inhibitory concentration. The MIC results were consistent with the results of the punching method. When Candida albicans was used as the target, the BAC treatment group had the best antibacterial effect, reaching 1.5 mg / ml, and when Staphylococcus aureus was used as the target, the minimum inhibitory concentrations of the blank group and the BA group both reached 0.3 mg / ml; the minimum inhibitory concentrations of the BC group and the BCA group reached 0.15 mg / ml.
[0075]
[0076] In summary, adding samples A, C, and AC to the sample soap can improve the antibacterial effect of the sample soap on Candida albicans and Staphylococcus aureus, among which the addition of sample C plays a key role in improving the antibacterial effect, and the simultaneous addition of AC can effectively improve the antibacterial effect of the sample soap on Candida albicans, and the minimum inhibitory concentration for Candida albicans reaches 1.56 mg / ml, and the minimum inhibitory concentration for Staphylococcus aureus reaches 0.156 mg / ml.
[0077] 5. Determination of the time-killing ability of sample soap BAC against pathogens
[0078] Experimental methods:
[0079] The time-killing ability of sample soap BAC against Staphylococcus aureus and Candida albicans was detected by referring to the method described in GB159979-2002. The specific scheme is as follows:
[0080] 1) Prepare the bacterial solution to be tested using the same method as above;
[0081] 2) Use PBS to dilute the melted soap solution to 0.5×MIC, 1×MIC, and 2×MIC, respectively. Then add 100μl of bacterial solution to 900μl of diluted sample soap solution, start timing, and take out samples for dilution at 30s, 1min, and 3min, respectively. Use PBS for dilution, and the dilution multiples are 10 -4 , 10 -5 , 10 -6 ; Then the colony counts of each group of samples were performed and the sterilization rate was calculated.
[0082] Sterilization rate = (blank - treatment group) blank group * 100%
[0083] Experimental results: As shown in the table below, the killing ability of sample soap BAC against Staphylococcus aureus and Candida albicans gradually increased with time. At the same time, we found that the time killing ability was different for different strains of bacteria. The killing rate of 2×MIC for Staphylococcus aureus reached 95% at 3 minutes, while it was only 92% for Candida albicans under the same conditions. This may be because the fungal cell structure is more complex than that of bacteria and is therefore more difficult to kill. This result is consistent with our previous test results.
[0084] ;
[0085] ;
[0086] The results show that ( Figure 8 ): Sample soap BAC has a strong inhibitory effect on Staphylococcus aureus and Candida albicans. The killing ability of sample soap BAC on Staphylococcus aureus and Candida albicans gradually increases with time. At the same time, we found that the time killing ability is different for different two strains of bacteria. The killing rate of 2×MIC for Staphylococcus aureus reaches 95% at 3min, while it is only 92% for Candida albicans under the same conditions. The killing rate of 2×MIC for Staphylococcus aureus reaches 92.86% at 30s, while it is only 86.50% for Candida albicans under the same conditions. Sample soap BAC is a better antibacterial soap for inhibiting Staphylococcus aureus and Candida albicans.
Claims
1. A method for preparing alpine rhodiola rosea double-effect nano cold-process soap, comprising: 1) Pour sodium hydroxide into a container containing nanosilver sol and cool it to 40°C to obtain nanosilver alkali solution; The weight ratio of sodium hydroxide to nano silver sol is 0.8-1.2:2.5; 2) Heat the oil to 60°C and then cool it to 40°C; 3) Pour 350-400g of nanosilver lye into 1000g of oil, stirring while adding; 4) Add 0.3-0.5g of alpine rhodiola rosea powder to every 1000g of oil; 5) Pour the soap solution into the mold gently and keep it warm for 24 hours before demoulding; 6) Place the demoulded soap in a cool and dry place and let it mature for 3-4 weeks before use.
2. The method for preparing the alpine rhodiola rosea double-effect nano cold-process soap according to claim 1, characterized in that: The oil comprises 25%-35% white oil, 25%-35% coconut oil, 15%-25% castor oil and 15%-25% canola oil in weight percentage, sodium hydroxide, nano silver sol and rhodiola rosea powder.
3. The method for preparing the alpine rhodiola rosea double-effect nano cold-process soap according to claim 2, characterized in that: The weight ratio of the sodium hydroxide to the nano silver sol in step 1) is 1:2.
5.
4. The method for preparing the alpine rhodiola rosea double-effect nano cold-process soap according to claim 3, characterized in that: In step 4), 0.4 g of alpine rhodiola rosea powder is added to every 1000 g of oil.
5. The method for preparing the alpine rhodiola rosea double-effect nano cold-process soap according to claim 1, 2, 3 or 4, characterized in that: The rhodiola rosea powder is prepared by the following method: 1) Weigh 20 g of dried roots of Rhodiola rosea, place them in an appropriate container, add 100 times the amount of distilled water and soak for 5 days; after soaking, filter with non-woven fabric and collect the filtrate and residue separately. 6.2) Place the filter residue in a stainless steel pot, add 30 times the amount of distilled water and boil for 20 minutes; after boiling, filter with non-woven fabric to collect the filtrate of Rhodiola rosea; 3) Mix the distilled water soaking filtrate and the decoction filtrate, and concentrate them using a rotary evaporator. Stop the operation when the volume of the concentrate reaches about 10 mL; freeze-dry to obtain the alpine Rhodiola rosea water extract, seal it in a brown reagent bottle, and place it in a 4°C refrigerator for later use; 4) Take a certain volume of macroporous resin D101 in a large beaker, slowly add 95% volume concentration ethanol solution into the beaker until the ethanol solution level exceeds the macroporous resin particles, let it stand for about 12 hours, rinse with distilled water until there is no smell, and then load it into the column. Load it to two-thirds of the glass column, prepare the alpine Rhodiola rosea water extract to a concentration of 5 mg / mL for sample loading, and the sample volume is one-third of the volume of the macroporous resin. After the loading is completed, let it stand for 12 hours, rinse with distilled water, 10% ethanol, 20% ethanol, and 80% ethanol in turn, collect the 80% ethanol elution component, concentrate the eluate, and dry it into powder, which is the Rhodiola rosea powder.
7. The method for preparing the alpine rhodiola rosea double-effect nano cold-process soap according to claim 5, characterized in that: The nano silver sol is prepared by the following method: 1) Rinse the purple cabbage with distilled water, dry it, chop it, weigh 50-60 grams, add 100 mL of distilled water, heat and boil for 5 minutes, cool to room temperature, and vacuum filter to obtain the purple water extract; 2) Dilute to 250 mL with distilled water and store at 4°C for later use; 3) Mix the purple cabbage water extract and 0.001 mol∙L -1 Silver nitrate is mixed in a volume ratio of 1-9:9-1, placed under a xenon lamp light source under magnetic stirring, and reacted for 1-40 minutes.
8. The method for green synthesis of nanosilver from purple cabbage extract according to claim 6, characterized in that: Light intensity is 1W∙cm 2 , react for 1 to 20 minutes.
9. The method for green synthesis of nanosilver from purple cabbage extract according to claim 7, characterized in that: The volume ratio is 8:2, and the reaction time is 20 minutes.