Sample smear preparation device for microbiological detection

Abstract

The sample smear preparation device comprises a sealing box, a drawing box is arranged on the side face of the sealing box, a sample storage mechanism is arranged in an inner cavity of the drawing box, a stand column is arranged in the middle of the inner cavity of the sealing box, and a motor is installed at the top of the stand column in a sinking mode. And a rotating strip is arranged on an output shaft of the motor in a sleeving mode, a hydraulic mechanism is arranged at the bottom of the rotating strip, a suction pipe is installed at the bottom of the hydraulic mechanism in a butt joint mode, and a pressing ring is arranged on the lower middle portion of the outer ring of the suction pipe. By means of the suction pipe with the pressing ring, the storage pipe, the hollow pipe and the structural design on the suction pipe and the storage pipe, when a microorganism sample is sucked, disturbance of a sample mixed solution can be conducted in a self-adaptive mode on the premise that the sample is isolated from the outside in the sampling process, and therefore the uniformity of the content of microorganisms in the smear sample is improved, and the sampling efficiency is improved. Therefore, the accuracy of detection data is improved.

Description

Technical Field

[0001] The invention relates to the technical field of microbial detection, in particular to a sample smear preparation device for microbial detection. Background Art

[0002] Microbial sample smears are widely used in the inoculation, cultivation, separation and detection of microorganisms, and are indispensable experimental materials in microbial analysis and detection. In actual operation, taking microbial sample collection as an example, the experimenter usually uses a sampling tool to dip a small amount of the sample to be tested and evenly spreads it on the surface of the slide to complete the smear preparation. However, the existing technology still has many technical problems in the process of microbial sample smears.

[0003] At present, there are related patent technologies to improve microbial sample smear tools. For example:

[0004] 1. A Chinese patent (CN205241683U) proposes a microbial sample smear pen, which includes a mechanical connection end and a free end, which are rigidly connected. The mechanical connection end is detachably connected to an external mechanical structure, while the free end is arranged parallel to the slide and is equipped with a medical fiber coating at the part in contact with the slide.

[0005] 2. A Chinese patent (CN109112060A) discloses an improved microbial sample smear pen, the structure of which includes a pen housing, a pen holder, a rotating wheel, a liquid pushing mechanism, a blocking mechanism, a pen head, a flow control mechanism and a moving mechanism to optimize the smear process.

[0006] However, both the traditional manual smear method and the above-mentioned improved mechanical smear scheme still have the following technical problems:

[0007] a. Limited operation accuracy and insufficient stability - manual operation is easily affected by human factors, and there are large errors in the smear quality, resulting in poor experimental repeatability, which in turn affects the accuracy of subsequent microbial testing;

[0008] b. Biological contamination risk: Microbial samples are exposed to the external environment during the smearing process, which may cause cross-contamination and affect the reliability of the experimental results. There is also a potential risk of operators being exposed to pathogenic microorganisms;

[0009] c. Changes in sample status - The time interval from sample collection to smearing may cause coagulation or precipitation of microbial samples, thereby affecting the uniform distribution of samples and the accuracy of subsequent experimental analysis.

[0010] Based on the above technical problems, the present application provides a sample smear preparation device for microbial detection to optimize the smear quality, improve operational stability, and reduce the risk of biological contamination, thereby improving the accuracy and reliability of microbial detection. Summary of the invention

[0011] The object of the present invention is to provide a sample smear preparation device for microbial detection to solve the problems raised in the above background technology.

[0012] To achieve the above object, the present invention provides the following technical solutions:

[0013] A sample smear preparation device for microorganism detection comprises a sealed box, a drawer box is arranged on the side of the sealed box, and a sample storage mechanism is arranged in the inner cavity of the drawer box.

[0014] A column is arranged in the middle of the inner cavity of the sealing box, a motor is installed in a sunken manner on the top of the column, a rotating bar is mounted on the output shaft of the motor, a hydraulic mechanism is arranged at the bottom of the rotating bar, a suction pipe is installed at the bottom of the hydraulic mechanism, and a pressure ring is arranged at the middle and lower part of the outer ring of the suction pipe.

[0015] The sample storage mechanism mainly includes a storage tube for accommodating a microbial mixture, a hollow tube is fixedly installed on the inner wall of the storage tube, a compression rod is installed through the inner cavity of the hollow tube, a sealing plug is fixedly installed at the bottom of the compression rod, a small spring is installed at the bottom of the sealing plug, and a limiting plate is fixedly installed on the top of the compression rod.

[0016] Preferably, the bottom of the hollow tube is an open structure, the bottom of the small spring is against the bottom of the inner wall of the storage tube, and a blocking piece is fixedly installed on the outer wall of the storage tube.

[0017] Preferably, the outer diameter of the suction tube is smaller than the inner diameter of the storage tube, a through hole structure for the mixed liquid to flow into is opened on the outer wall of the suction tube, and the outer diameter of the pressure ring is the same as the inner diameter of the storage tube.

[0018] Preferably, a tooth structure is arranged at equal distances on the inner wall of the pull-out box, a notch structure is provided on the outer wall of the suction tube, an annular groove is provided on the inner wall of the suction tube and behind the notch structure, and a toothed disc is clamped in the inner cavity of the annular groove.

[0019] Preferably, a mixing shaft is fixedly installed in the middle of the bottom of the toothed disc, and a stirring blade is arranged at the bottom of the outer side wall of the mixing shaft.

[0020] Preferably, a strip groove is provided at the top of the rotating bar, a limit rod is fixedly installed in the inner cavity of the strip groove, a push spring is mounted on the outer ring of the limit rod, and a limit block is installed on the top of the hydraulic mechanism, and the limit block is mounted on the outer ring of the limit rod.

[0021] Preferably, a recessed area is provided at the top of the inner wall of the suction tube, a movable plate is clamped in the inner cavity of the recessed area, the top of the movable plate is docked and installed with the telescopic rod structure of the hydraulic mechanism, a smear spring is installed on the top of the movable plate, a smear head is installed at the bottom of the suction tube, a rolling ball structure is rotatably installed in the inner cavity of the smear head, and a groove structure for accommodating the sample solution is provided on the outer wall of the rolling ball structure.

[0022] Preferably, a slope structure is provided at the bottom of the inner wall of the pull-out box, an inner plate is fixedly mounted at the bottom of the inner wall of the pull-out box, and brackets for placing glass slides are provided on the inner plate and the inner wall of the pull-out box.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] 1. In the sample smear preparation device for microbial detection, the entire microbial mixed liquid sample and the smear environment are in a closed state, and no manual operation is required for smearing, thereby effectively solving the problems of errors in manual smearing and susceptibility to secondary contamination when exposed to the external environment.

[0025] 2. The sample smear preparation device for microbial detection, through the suction tube with a pressure ring, the storage tube, the hollow tube and the structural design thereon, can adaptively disturb the sample mixture during the sampling process while maintaining isolation from the outside world when sucking the microbial sample, thereby improving the uniformity of the microbial content in the smear sample, that is, improving the accuracy of the detection data in disguise.

[0026] 3. The sample smear preparation device for microbiological detection is designed and used with a continuously rotating suction tube, toothed disc, mixing shaft, stirring blade and tooth structure. When the smear microbiological sample is transferred, the toothed disc, mixing shaft and stirring blade rotate, and the sample mixture is always stirred and mixed just before the smear. This not only maintains a uniform distribution of microorganisms in the smear sample, but also solves the problems of sample agglomeration, flocculation and precipitation, thereby improving the quality of the smear, that is, improving the accuracy and credibility of the comparison of each group of data in subsequent tests.

[0027] 4. This sample smear preparation device for microbial detection uses a multi-layer slide placement solution and is used in conjunction with an adaptive up and down suction tube. It can coat multiple groups of slides with the same batch of sample liquid on the premise of uniform sample liquid, thereby ensuring that multiple groups of test samples are prepared in the same environment, thereby further improving the feasibility and accuracy of subsequent test data and comparison data. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1It is a schematic diagram of the structure of the present invention;

[0029] Figure 2 This is a structural assembly diagram of the present invention;

[0030] Figure 3 It is a schematic diagram of the structure of the sample storage mechanism of the present invention;

[0031] Figure 4 This is a schematic diagram of the structure of the drawer box of the present invention;

[0032] Figure 5 This is a schematic diagram of the sealing box structure of the present invention;

[0033] Figure 6 It is a cross-sectional view of the suction tube structure of the present invention.

[0034] In the figure: 1. Sealing box; 11. Column; 12. Motor; 13. Rotating bar; 14. Limiting block; 15. Hydraulic mechanism; 16. Limiting rod; 17. Pushing spring; 18. Suction tube; 181. Smear head; 182. Recessed area; 183. Movable plate; 184. Smear spring; 185. Notch structure; 186. Toothed disc; 187. Mixing shaft; 188. Stirring blade; 189. Pressing ring; 2. Pull-out box; 21. Inner plate; 22. Bracket; 23. Slope structure; 24. Tooth structure; 3. Sample storage mechanism; 31. Storage tube; 32. Blocking piece; 33. Hollow tube; 34. Compression rod; 35. Sealing plug; 36. Small spring; 37. Limiting piece. DETAILED DESCRIPTION

[0035] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0036] See also Figure 1-6 The present invention provides a technical solution for a sample smear preparation device for microbial detection:

[0037] Embodiment 1:

[0038] The device mainly comprises a sealed box 1, a draw-out opening is provided at the side of the sealed box 1, a draw-out box 2 is inserted into the inner cavity of the draw-out opening, and a sample storage mechanism 3 is arranged in the inner cavity of the draw-out box 2.

[0039] A column 11 is arranged in the inner cavity of the sealing box 1, a motor 12 is sunken installed on the top of the column 11, a rotating bar 13 is mounted on the output shaft of the motor, a hydraulic mechanism 15 is arranged at the bottom position of the rotating bar 13, a suction pipe 18 is docked and installed at the bottom of the hydraulic mechanism 15, and a pressure ring 189 is arranged at the middle and lower layer position of the outer side wall of the suction pipe 18.

[0040] The sample storage mechanism 3 mainly includes a storage tube 31, on the inner wall of which a hollow tube 33 is fixedly installed, in the inner cavity of the hollow tube 33 a compression rod 34 is inserted through, at the bottom of the compression rod 34 a sealing plug 35 is installed, at the bottom of the sealing plug 35 a small spring 36 is fixedly bonded, and at the top of the compression rod 34 a limiting plate 37 is fixedly installed.

[0041] The bottom of the hollow tube 33 is an open structure, and the bottom of the small spring 36 is against the bottom of the inner wall of the storage tube 31 .

[0042] The outer diameter of the suction tube 18 is smaller than the inner diameter of the storage tube 31 , and a through hole for the sample to enter is opened on the outer wall of the suction tube 18 . The outer diameter of the pressure ring 189 is the same as the inner diameter of the storage tube 31 .

[0043] A baffle 32 is fixedly mounted on the outer wall of the storage tube 31. When the drawer box 2 is initially placed, it is necessary to ensure that the suction tube 18 is located in front of the drawer opening, so that the baffle 32 can be used to keep the suction tube 18 next to the storage tube 31 during the placement of the drawer box.

[0044] In this embodiment, when in use, the sample mixture obtained by sampling is placed in the storage tube 31, and then the pull-out box 2 with the sample mixture is directly inserted into the sealed box 1, and sealing strips are provided on the sides of the pull-out box 2 and the sides of the pull-out opening. After the pull-out box 2 is placed, the entire device remains in a sealed state.

[0045] Then the smearing action is performed, that is, the motor 12 and the hydraulic mechanism 15 are started. The hydraulic mechanism 15 continuously contracts upward to drive the entire suction tube 18 to move upward until it reaches the highest position. At this time, the lowest end of the suction tube 18 is higher than the upper end of the storage tube 31. Then the motor 12 drives the rotating bar 13 together with the hydraulic mechanism 15 and the suction tube 18 to move to the position just above the storage tube 31. Then the entire suction tube 18 is continuously pushed into the inner cavity of the storage tube 31 through the hydraulic mechanism 15. In the process of lowering the suction tube 18, the pressure ring 189 gradually squeezes The limiting plate 37 continuously pushes the compression rod 34 and the sealing plug 35 downward. When the sealing plug 35 moves downward, the mixed liquid remaining in the inner cavity of the hollow tube 33 is pushed into the storage tube 31. When pushing, due to the action of force, the mixed liquid discharged from the hollow tube 33 will impact the mixed liquid in the storage tube 31, thereby achieving the purpose of disturbing the sample. As the suction tube 18 continues to go deeper, the disturbed sample mixed liquid flows into the suction tube 18 from the through hole, and finally the suction tube is drawn out of the storage tube 31 through the hydraulic mechanism 15 again.

[0046] In the present technical solution, through the suction tube 18 with a pressure ring 189, the storage tube 31, the hollow tube 33 and the structural design thereon, when aspirating the microbial sample, the sample mixture can be adaptively disturbed while being isolated from the outside world during the sampling process, thereby improving the uniformity of the microbial content in the smear sample, that is, improving the accuracy of the detection data in disguise.

[0047] Embodiment 2:

[0048] A tooth structure 24 is equidistantly arranged on the upper position of the inner wall of the pull-out box 2, a notch structure 185 is opened on the side of the suction tube 18, an annular groove is arranged on the inner wall of the suction tube 18 and behind the notch structure 185, a toothed disc 186 is clamped in the inner cavity of the annular groove, a mixing shaft 187 is installed in the middle of the bottom of the toothed disc 186, and a stirring blade 188 is arranged at the bottom of the outer side of the mixing shaft 187.

[0049] Among them, a strip groove is opened at the top of the rotating bar 13, a limit rod 16 is installed in the inner cavity of the strip groove, a push spring 17 is mounted on the outer ring of the limit rod 16, and a limit block 14 is installed on the top of the hydraulic mechanism 15, and the limit block 14 is mounted on the outer rings of the two limit rods 16.

[0050] In this embodiment, based on the embodiment 1, after the suction tube 18 takes out the smear sample and detaches from the storage tube 31, the push spring 17 loses its force restraint, and pushes the hydraulic mechanism 15 and the suction tube 18 to move close to the inner wall of the pull-out box 2 until the pressure ring 189 rests on the inner wall of the pull-out box 2. At this time, the tooth structure 24 is inserted into the notch structure 185 and meshes with the toothed disc 186. Then, driven by the motor 12, the suction tube 18 starts to move in a circular motion, and during the movement, the toothed disc 186 rotates in the annular groove, driving the mixing shaft 187 and the stirring blade 188 at its bottom to rotate. During the movement of the suction tube 18, the sucked sample can be stirred and mixed at all times. On the one hand, the internal microorganisms can be kept evenly distributed, and on the other hand, the problem of agglomeration or flocculation of the substances in the sample can be avoided.

[0051] In the present technical solution, through the design and use of the continuously rotating suction tube 18, the toothed disc 186, the mixing shaft 187, the stirring blade 188 and the tooth structure 24, when transferring the smear microbial sample, the toothed disc 186, the mixing shaft 187 and the stirring blade 188 rotate, and the sample mixture is always stirred and mixed just before the smear, which not only maintains the uniform distribution of microorganisms in the smear sample, but also solves the problems of sample agglomeration, flocculation and precipitation, thereby improving the quality of the smear, that is, improving the accuracy and credibility of the comparison of each group of data in subsequent tests.

[0052] Embodiment 3:

[0053] A recessed area 182 is provided at the top of the inner wall of the suction tube 18, a movable plate 183 is clamped in the inner cavity of the recessed area 182, and the top of the movable plate 183 is docked and installed with the telescopic rod structure of the hydraulic mechanism 15, and a smear spring 184 is installed at the outer edge of the top of the movable plate 183.

[0054] A smear head 181 is installed at the bottom of the suction tube 18 , a rolling ball structure is clamped at the bottom of the inner cavity of the smear head 181 , and a groove structure for accommodating the sample liquid is arranged on the outer wall of the rolling ball structure.

[0055] A slope structure 23 is provided at the bottom of the inner wall of the pull-out box 2 , an inner plate 21 is provided at the bottom of the inner wall of the pull-out box 2 , and a bracket 22 for placing a slide is provided in a stepped manner on the inner plate 21 and the inner wall of the pull-out box 2 .

[0056] In this embodiment, based on Embodiment 1 and Embodiment 2, when the suction tube 18 climbs to the top along the ramp structure 23, after continuing to move, the smear head 181 begins to contact the glass slide, and cooperates with the reverse force of the smear spring 184, so that the smear head 181 can always have a certain force between the glass slide, so the smear head 181 will rotate when moving, and the sample mixture in the groove structure is applied to the glass slide. The stepped glass slide design can be immediately lowered to the next glass slide for smearing after one is coated, that is, multiple groups of smears can be performed with the same batch of test samples in the same cycle, without any interference from other variables. In this technical solution, in order to ensure the quality of the smear, try to perform two to three smear operations.

[0057] In the present technical solution, a multi-layer slide placement solution is used in conjunction with an adaptive up and down suction tube 18, so that multiple groups of slides can be coated with the same batch of sample liquid on the premise of uniform sample liquid, thereby ensuring that multiple groups of test samples are prepared in the same environment, thereby further improving the feasibility and accuracy of subsequent test data and comparison data.

[0058] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A sample smear preparation device for microbial detection, comprising a sealed box (1), characterized in that: A drawer box (2) is provided on the side of the sealed box (1), and a sample storage mechanism (3) is provided in the inner cavity of the drawer box (2); A column (11) is provided in the middle of the inner cavity of the sealing box (1), a motor (12) is installed in a sunken manner on the top of the column (11), a rotating bar (13) is mounted on the output shaft of the motor (12), a hydraulic mechanism (15) is provided at the bottom of the rotating bar (13), a suction pipe (18) is installed at the bottom of the hydraulic mechanism (15), and a pressure ring (189) is provided at the middle and lower part of the outer ring of the suction pipe (18); The sample storage mechanism (3) mainly comprises a storage tube (31) for accommodating a microbial mixture, a hollow tube (33) is fixedly mounted on the inner wall of the storage tube (31), a compression rod (34) is installed through the inner cavity of the hollow tube (33), a sealing plug (35) is fixedly mounted on the bottom of the compression rod (34), a small spring (36) is installed on the bottom of the sealing plug (35), and a limiting plate (37) is fixedly mounted on the top of the compression rod (34).

2. A sample smear preparation device for microbial detection according to claim 1, characterized in that: The bottom of the hollow tube (33) is an open structure, the bottom of the small spring (36) is against the bottom of the inner wall of the storage tube (31), and a blocking sheet (32) is fixedly mounted on the outer wall of the storage tube (31).

3. A sample smear preparation device for microorganism detection according to claim 1, characterized in that: The outer diameter of the suction tube (18) is smaller than the inner diameter of the storage tube (31); a through hole structure for the mixed liquid to flow into is provided on the outer wall of the suction tube (18); and the outer diameter of the pressure ring (189) is the same as the inner diameter of the storage tube (31).

4. A sample smear preparation device for microorganism detection according to claim 1, characterized in that: A tooth structure (24) is arranged at equal distances on the inner wall of the pull-out box (2), a notch structure (185) is provided on the outer wall of the suction tube (18), an annular groove is provided on the inner wall of the suction tube (18) and at the rear of the notch structure (185), and a toothed disc (186) is clamped in the inner cavity of the annular groove.

5. A sample smear preparation device for microorganism detection according to claim 4, characterized in that: A mixing shaft (187) is fixedly mounted in the middle of the bottom of the toothed disc (186), and a stirring blade (188) is arranged at the bottom of the outer side wall of the mixing shaft (187).

6. A sample smear preparation device for microorganism detection according to claim 1, characterized in that: A strip groove is provided at the top of the rotating bar (13), a limit rod (16) is fixedly installed in the inner cavity of the strip groove, a push spring (16) is sleeved on the outer ring of the limit rod (16), and a limit block (14) is installed on the top of the hydraulic mechanism (15), and the limit block (14) is sleeved on the outer ring of the limit rod (16).

7. A sample smear preparation device for microorganism detection according to claim 1, characterized in that: A recessed area (182) is provided at the top of the inner wall of the suction tube (18), a movable plate (183) is clamped in the inner cavity of the recessed area (182), the top of the movable plate (183) is docked and installed with the telescopic rod structure of the hydraulic mechanism (15), a smear spring (184) is installed on the top of the movable plate (183), a smear head (181) is installed at the bottom of the suction tube (18), a rolling ball structure is rotatably installed in the inner cavity of the smear head (181), and a groove structure for accommodating a sample solution is provided on the outer wall of the rolling ball structure.

8. A sample smear preparation device for microorganism detection according to claim 1, characterized in that: The bottom of the inner wall of the pull-out box (2) is provided with a slope structure (23), the bottom of the inner wall of the pull-out box (2) is fixedly mounted with an inner side plate (21), and the inner side plate (21) and the inner side wall of the pull-out box (2) are both provided with a bracket (22) for placing a glass slide.

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