A peristaltic pump for ultrafiltration of varicella-zoster virus liquid and an ultrafiltration process

Abstract

The present invention discloses a peristaltic pump for ultrafiltration of varicella-zoster virus liquid and an ultrafiltration process, which relates to the technical field of biopharmaceuticals and includes an energy-saving motor, a main wheel, a backup wheel, a vertical seat, and a pump tube. An extension cylinder is fixedly arranged on the rear side surface of the vertical seat, and the energy-saving motor is fixedly connected to the side surface of the extension cylinder. In the present invention, a set of main wheels and a set of backup wheels are arranged inside the peristaltic pump housing, and a switching component is arranged inside the peristaltic pump housing. Under normal conditions, the main wheels perform the peristaltic work of the peristaltic pump. When the main wheels of the peristaltic pump are damaged, the user can quickly remove the main wheels from the peristaltic position through the switching component, and then place the secondary wheels at the peristaltic position, avoiding long-term downtime waiting, effectively improving the working continuity and production efficiency of the equipment, ensuring that the peristaltic pump can resume work as soon as possible when a failure occurs, and also reducing the dependence on highly skilled workers, making the maintenance work simpler and more efficient.

Description

Technical Field

[0001] The present invention relates to the technical field of biopharmaceuticals, and particularly relates to a peristaltic pump for ultrafiltration of varicella-zoster virus liquid and an ultrafiltration process. Background Art

[0002] Varicella-zoster virus liquid ultrafiltration requires the use of a peristaltic pump. This is mainly because the ultrafiltration process requires filtering macromolecular substances such as proteins and virus particles in the virus liquid through a semi-permeable membrane to achieve separation and concentration. The peristaltic pump can provide stable pressure and flow during the ultrafiltration process, ensuring that the liquid passes through the ultrafiltration membrane evenly, effectively promoting the contact between the liquid and the membrane surface, improving the filtration efficiency, maintaining the operating pressure of the membrane, preventing the filtration speed from decreasing, and helping to remove contaminants on the membrane surface, ensuring the continuity and high efficiency of the ultrafiltration process.

[0003] In a traditional peristaltic pump, generally only one set of pressing wheels is provided inside. There are usually more than two pressing wheels in this set. When this set of pressing wheels needs to be replaced due to damage, the peristaltic pump needs to be shut down at this time. Workers first need to remove the old pressing wheels, and then install the new pressing wheels inside the peristaltic pump, which involves multiple steps and takes a long time as a whole, seriously affecting the normal operation of the pump. Moreover, the disassembly and installation process requires a high level of professionalism from workers. Improper operation may cause equipment damage or improper installation, increasing the maintenance difficulty and cost. Summary of the Invention

[0004] The purpose of the present invention is to solve the problem that in the prior art, generally only one set of pressing wheels is provided inside the peristaltic pump, and there are usually more than two pressing wheels in this set. When this set of pressing wheels needs to be replaced due to damage, the peristaltic pump needs to be shut down at this time. Workers first need to remove the old pressing wheels, and then install the new pressing wheels inside the peristaltic pump, which involves multiple steps and takes a long time as a whole, seriously affecting the normal operation of the pump. Moreover, the disassembly and installation process requires a high level of professionalism from workers. Improper operation may cause equipment damage or improper installation, increasing the maintenance difficulty and cost. Therefore, a peristaltic pump for ultrafiltration of varicella-zoster virus liquid and an ultrafiltration process are proposed.

[0005] In order to achieve the above purpose, the present invention adopts the following technical scheme:

[0006] A peristaltic pump for ultrafiltration of varicella-zoster virus liquid, comprising an energy-saving motor, a main wheel, a replacement wheel, a vertical seat, and a pump tube. An extension cylinder is fixedly arranged on the rear side surface of the vertical seat, the energy-saving motor is fixedly connected to the side surface of the extension cylinder, a limiting component for fixing and limiting the pump tube is arranged on the vertical seat on the opposite side of the energy-saving motor, the limiting component includes a fixing ring, a left fixing ring, a right fixing ring, and a quick-fixing component, and the quick-fixing component realizes the quick disassembly and assembly between the left fixing ring and the right fixing ring;

[0007] An extension post is fixedly arranged at the output end of the energy-saving motor. A one-word rod is fixedly arranged at the end of the extension post. The one-word rod is horizontally inserted into a horizontal cylinder in a movable manner. A rectangular plate is fixedly arranged on the outer wall of the horizontal cylinder. Guide grooves are symmetrically arranged on the rectangular plate in the vertical direction. Guide posts are movably inserted into the guide grooves. A first connecting post and a second connecting post are sequentially and fixedly arranged on the outer wall of the guide post in the horizontal direction. A main wheel is rotatably connected to the end of the first connecting post. A substitute wheel is rotatably connected to the end of the second connecting post. A switching assembly for switching the main wheel or the substitute wheel to the peristaltic working position is arranged on the side of the second connecting post. The switching assembly includes a pushing cylinder, a driving rod, a limiting assembly and an indicating assembly. The limiting assembly is used for limiting the main wheel or the substitute wheel at the peristaltic working position. The indicating assembly indicates that the main wheel or the substitute wheel is at the peristaltic working position.

[0008] Optionally, a left fixing ring is fixedly arranged on the side of the vertical seat. Two fixing rings are fixedly arranged on the vertical seat on one side of the left fixing ring in the vertical direction. A rectangular groove is arranged at the middle position on the side of the left fixing ring. A rectangular column is fixedly arranged on the side of the right fixing ring. The rectangular column is movably inserted into the interior of the rectangular groove. A left fixing groove is arranged on the side of the left fixing ring. A right fixing groove is arranged on the side of the right fixing ring. The quick-fixing assembly includes a threaded column, a locking grip and a quick-fixing block.

[0009] Optionally, one end of the threaded column is fixedly connected to the side of the vertical seat. A quick-fixing block is movably inserted outside the threaded column. A locking grip is screwed onto the outer wall at the end of the threaded column. A limiting groove is arranged on the side of the quick-fixing block. The total width of the limiting groove is greater than the total thickness of the vertical seat, the left fixing ring and the right fixing ring.

[0010] Optionally, a pushing cylinder is screwed onto the outer wall of the horizontal cylinder. An auxiliary nut is fixedly arranged at the end of the outer wall of the pushing cylinder. A receiving groove is arranged on the outer wall of the pushing cylinder. A driven ring is rotatably connected to the interior of the receiving groove. Driving rods are symmetrically and rotatably connected to the outer wall of the driven ring. The remaining ends of the driving rods are rotatably connected to the side of the second connecting post.

[0011] Optionally, the limiting assembly includes a limiting cylinder, a first limiting ring, a second limiting ring and a limiting block. A triangular plate is fixedly arranged at one end of the guide post. An extension rod is fixedly arranged at the other end of the triangular plate. A limiting block is fixedly arranged at the other end of the extension rod. The limiting cylinder is concentrically and fixedly arranged on the side of the extension cylinder.

[0012] Optionally, a first limiting ring and a second limiting ring are sequentially and fixedly arranged in the inner cavity of the limiting cylinder. The distance between the end of the limiting cylinder and the first limiting ring is equal to the distance between the first limiting ring and the second limiting ring. The limiting block extends into the inner cavity of the limiting cylinder. The width of the limiting block is equal to the distance between the first limiting ring and the second limiting ring.

[0013] Optionally, the indicating component includes a U-shaped rod, a positioning post, a first abutting ring, and a second abutting ring. A positioning post is fixedly arranged at one end of the T-shaped rod away from the energy-saving motor. A U-shaped rod is fixedly arranged at the end of the horizontal cylinder. The positioning post passes through the center position of the U-shaped rod. The first abutting ring and the second abutting ring are fixedly arranged on both sides of the U-shaped rod in sequence along the positioning post.

[0014] Optionally, a transparent cover is fixedly arranged on the outside of the right fixing ring by bolts, and a central hole is opened at the center position of the side surface of the transparent cover.

[0015] Optionally, rear brackets are symmetrically and fixedly arranged on the rear side surface of the vertical seat. A support seat is fixedly arranged at the bottom between the two rear brackets. The top of the support seat is fixedly arranged at the bottom of the energy-saving motor.

[0016] An ultrafiltration process for varicella-zoster virus liquid includes the following steps:

[0017] S1, feeding stage:

[0018] Store the varicella-zoster virus liquid in a dedicated sterile storage tank to ensure that the virus liquid is stored under low-temperature and constant conditions to maintain its activity. Quantitatively transport the virus liquid through this peristaltic pump so that it enters the ultrafiltration module in the prior art. Set the rotation speed parameter of the peristaltic pump to control the feeding flow rate, so that the virus liquid enters the ultrafiltration system stably and uniformly, thereby ensuring the continuity and stability of the filtration process and avoiding affecting the filtration efficiency and the integrity of virus particles due to flow rate fluctuations.

[0019] S2, ultrafiltration stage:

[0020] The ultrafiltration module uses a hollow fiber ultrafiltration membrane as the core filtration medium. By adjusting the ultrafiltration pressure and the transmembrane flow rate, the virus liquid forms a tangential flow on the membrane surface. During this process, the flow rate, pressure, and temperature of the filtrate are monitored in real time to ensure the stability of the ultrafiltration process, and the operation parameters are adjusted in a timely manner according to the actual situation to ensure obtaining a virus liquid with high purity and high concentration.

[0021] Compared with the prior art, the present invention has the following advantages:

[0022] 1. In the present invention, a set of main wheels and a set of substitute wheels are arranged inside the peristaltic pump housing, and a switching component is arranged inside the peristaltic pump housing. Under normal conditions, the main wheels perform the peristaltic work of the peristaltic pump. When the main wheels of the peristaltic pump are damaged, the user can quickly remove the main wheels from the peristaltic working position through the switching component, and then place the substitute wheels at the peristaltic working position, avoiding long-term downtime waiting, effectively improving the working continuity and production efficiency of the equipment, ensuring that the peristaltic pump can resume work as soon as possible when a failure occurs, thereby reducing the production interruption time caused by equipment failures, ensuring the efficient operation of the production line, reducing the time for manual disassembly and installation, saving maintenance costs and labor costs, and also reducing the dependence on highly skilled workers, making the maintenance work simpler and more efficient.

[0023] 2. One of the core components in the switching component of the present invention is the limit component. First, the limit component ensures the correct position of the main wheels, enabling the substitute wheels to be accurately moved to the position where the pump tube needs to be pressed. Such a design ensures the consistency and accuracy of each switching operation, helps improve the working efficiency and performance of the equipment. In addition, when the user operates the switching component, after the switching component forces the main wheels to move away from the pump tube first, the main wheels can be removed, and then the substitute wheels move to the position where the pump tube needs to be pressed, forcibly restricting the operation sequence during the switching process, avoiding conflicts or damage between the main wheels and the pump tube, ensuring that each operation is carried out at a predetermined position, and reducing the risk of equipment failures.

[0024] 3. One of the core components in the switching component of the present invention is the indicating component. The indicating component is composed of a pure mechanical structure and extends from the side of the peristaltic pump housing. When the worker operates the main wheels and the substitute wheels using the switching component, specifically when using the limit component to remove the main wheels and the substitute wheels move to the pump tube position, the worker can quickly determine whether the main wheels or the substitute wheels are in the peristaltic position with the help of the indicating component. The worker can directly understand whether the main wheels or the substitute wheels are in the correct peristaltic position through the indicating component. This intuitive indication method greatly simplifies the operation process, improves the convenience of the worker's operation, reduces the operation difficulty, and also reduces the dependence on highly skilled workers, making the maintenance work simpler and more efficient. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a schematic diagram of the overall structure of the present invention.

[0026] Figure 2 is Figure 1 a semi-exploded structure schematic diagram of

[0027] Figure 3 is Figure 2 a schematic diagram of another perspective structure of

[0028] Figure 4 is Figure 2 a top view structure schematic diagram of

[0029] Figure 5 Another perspective structural schematic diagram of Figure 1 .

[0030] Figure 6 Structural schematic diagram of a quick-fixing component.

[0031] Figure 7 Structural schematic diagram of an extension cylinder and its connecting piece.

[0032] Figure 8 Structural schematic diagram of a switching component and its connecting piece.

[0033] Figure 9 Half-sectional structural schematic diagram of a limiting cylinder.

[0034] Figure 10 Structural schematic diagram of a switching component.

[0035] Figure 11 Another perspective structural schematic diagram of Figure 10 .

[0036] Figure 12 Structural schematic diagram of a horizontal cylinder and its connecting piece.

[0037] In the figure: 1, fixed ring; 2, vertical seat; 3, left fixing ring; 4, rectangular groove; 5, right fixing ring; 51, rectangular column; 52, right fixing groove; 53, pump pipe; 6, transparent cover; 61, central hole; 7, rear support frame; 8, energy-saving motor; 81, extension column; 810, cross bar; 9, extension cylinder; 10, support seat; 11, left fixing groove; 12, threaded column; 13, locking grip; 14, quick-fixing block; 141, limiting groove; 15, limiting cylinder; 151, second limiting ring; 152, first limiting ring; 16, limiting block; 17, extension rod; 18, triangular plate; 19, guiding column; 20, first connecting column; 21, second connecting column; 22, main wheel; 220, backup wheel; 23, driving rod; 24, pushing cylinder; 241, accommodating groove; 25, driven ring; 26, auxiliary nut; 27, rectangular plate; 271, guiding groove; 28, horizontal cylinder; 281, cross groove; 29, U-shaped rod; 30, positioning column; 301, first abutting ring; 302, second abutting ring. Specific embodiments

[0038] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments.

[0039] In the description of the present invention, it should be understood that the orientation or positional relationship indicated by the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present invention.

[0040] Referring to Figure 1-12 , a peristaltic pump for ultrafiltration of varicella-zoster virus liquid, which includes an energy-saving motor 8, a main wheel 22, a backup wheel 220, a vertical seat 2, and a pump tube 53. An extension cylinder 9 is fixedly arranged on the rear side surface of the vertical seat 2, and the energy-saving motor 8 is fixedly connected to the side surface of the extension cylinder 9. Selecting the energy-saving motor 8 as the power source of the peristaltic pump can significantly reduce energy consumption, improve operation efficiency, reduce equipment heating, and extend service life. The model of the energy-saving motor 8 is selected as a series of servo motors such as MSMD012G1U, which has the characteristics of high efficiency and energy saving, smooth start, high control precision, and is suitable for high-demand scenarios such as ultrafiltration of virus liquid. Compared with traditional motors, the energy-saving motor 8 can reduce energy consumption, lower operation costs, and has better overload capacity, adapting to long-term stable operation. Its low-noise and low-vibration characteristics help to maintain the stability of the laboratory or biopharmaceutical environment.

[0041] Rear support frames 7 are symmetrically and fixedly arranged on the rear side surface of the vertical seat 2. A support seat 10 is fixedly arranged at the bottom between the two rear support frames 7, and the top of the support seat 10 is fixedly arranged at the bottom of the energy-saving motor 8. The rear support frames 7 are used for the stable support of the vertical seat 2. A limiting component for fixing and limiting the pump tube 53 is arranged on the opposite side of the vertical seat 2 to the energy-saving motor 8. The limiting component includes a fixing ring 1, a left fixing ring 3, a right fixing ring 5, and a quick-fixing component. The quick-fixing component realizes the quick disassembly and assembly between the left fixing ring 3 and the right fixing ring 5.

[0042] An extension column 81 is fixedly arranged at the output end of the energy-saving motor 8. A one-way rod 810 is fixedly arranged at the end of the extension column 81. The one-way rod 810 is horizontally inserted into a horizontal cylinder 28 in an activity manner. A rectangular plate 27 is fixedly arranged on the outer wall of the horizontal cylinder 28. Guide grooves 271 are symmetrically opened in the vertical direction on the rectangular plate 27. Guide columns 19 are inserted into the guide grooves 271 in an activity manner. First connecting columns 20 and second connecting columns 21 are fixedly arranged on the outer wall of the guide column 19 in the horizontal direction in sequence. The guide column 19 is a cuboid, and the guide column 19 has the same width as the guide groove 271. A one-way groove 281 is arranged on the side surface of the horizontal cylinder 28. The cross-sectional dimension of the one-way groove 281 is the same as the cross-sectional dimension of the one-way rod 810. The cooperation between the one-way rod 810 and the one-way groove 281 enables the energy-saving motor 8 to drive the upper structure of the horizontal cylinder 28 to rotate, while the horizontal cylinder 28 can move along the axis direction of the one-way rod 810.

[0043] At the end of the first connecting post 20, a main wheel 22 is rotatably connected. At the end of the second connecting post 21, a replacement wheel 220 is rotatably connected. On the side of the second connecting post 21, there is a switching component for switching the main wheel 22 or the replacement wheel 220 to the peristaltic working position. The switching component includes a pushing cylinder 24, a driving rod 23, a limiting component, and an indicating component. The limiting component is used to limit the main wheel 22 or the replacement wheel 220 to the peristaltic working position, and the indicating component indicates that the main wheel 22 or the replacement wheel 220 is in the peristaltic working position. For the peristaltic pump for ultrafiltration of virus liquid, the material selection of its pressing wheel needs to meet corrosion resistance, wear resistance, and chemical compatibility. Therefore, the materials of the main wheel 22 and the replacement wheel 220 can be selected as stainless steel, rubber-coated stainless steel, etc.

[0044] On the side of the vertical seat 2, a left fixing ring 3 is fixedly arranged. In the middle position on the side of the left fixing ring 3, a rectangular groove 4 is opened. On the side of the right fixing ring 5, a rectangular column 51 is fixedly arranged, and the rectangular column 51 is movably inserted into the interior of the rectangular groove 4. On the side of the left fixing ring 3, a left fixing groove 11 is opened. On the side of the right fixing ring 5, a right fixing groove 52 is opened. The quick-fixing component includes a threaded column 12, a locking grip 13, a quick-fixing block 14. One end of the threaded column 12 is fixedly connected to the side of the vertical seat 2. The outside of the threaded column 12 is movably inserted with the quick-fixing block 14, and the part of the threaded column 12 in contact with the quick-fixing block 14 is a smooth rod part.

[0045] At the end of the outer wall of the threaded column 12, the locking grip 13 is screwed in. On the side of the quick-fixing block 14, a limiting groove 141 is opened. The total width of the limiting groove 141 is greater than the total thickness of the vertical seat 2, the left fixing ring 3, and the right fixing ring 5. The depth and shape of the left fixing groove 11 and the right fixing groove 52 are set appropriately. When the limiting groove 141 of the quick-fixing block 14 is stuck to the tops of the vertical seat 2, the left fixing ring 3, and the right fixing ring 5, the left fixing ring 3 and the right fixing ring 5 can wrap the outer wall of the pump tube 53, and the inner wall of the pump tube 53 will be exposed, which is convenient for the main wheel 22 and the replacement wheel 220 to press into the interior of the pump tube 53. And on one side of the left fixing ring 3 of the vertical seat 2, two fixing rings 1 are fixedly arranged along the vertical direction, and the two fixing rings 1 can be used to assist in fixing both ends of the pump tube 53. When the pump tube 53 needs to be replaced, the locking grip 13 is loosened manually, the quick-fixing block 14 can rotate around the threaded column 12, and the limiting groove 141 of the quick-fixing block 14 no longer gets stuck to the tops of the vertical seat 2, the left fixing ring 3, and the right fixing ring 5. At this time, the right fixing ring 5 can be separated from the left fixing ring 3.

[0046] On the outer wall of the horizontal cylinder 28, the pushing cylinder 24 is screwed in. At the end of the outer wall of the pushing cylinder 24, an auxiliary nut 26 is fixedly arranged. On the outer wall of the pushing cylinder 24, a receiving groove 241 is opened. Inside the receiving groove 241, a driven ring 25 is rotatably connected. Symmetrically on the outer wall of the driven ring 25, the driving rods 23 are rotatably connected. The remaining ends of the driving rods 23 are rotatably connected to the side of the second connecting post 21, and the driving rods 23 are in an inclined state.

[0047] The limiting component includes a limiting cylinder 15, a second limiting ring 152, a first limiting ring 151, and a limiting block 16. One end of the guiding column 19 is fixedly provided with a triangular plate 18, the other end of the triangular plate 18 is fixedly provided with an extension rod 17, and the other end of the extension rod 17 is fixedly provided with the limiting block 16. The limiting cylinder 15 is concentrically and fixedly arranged on the side surface of the extension cylinder 9.

[0048] The first limiting ring 152 and the second limiting ring 151 are sequentially and fixedly arranged in the inner cavity of the limiting cylinder 15. The distance between the end of the limiting cylinder 15 and the first limiting ring 152 is equal to the distance between the first limiting ring 152 and the second limiting ring 151. The limiting block 16 extends into the inner cavity of the limiting cylinder 15, and the width of the limiting block 16 is equal to the distance between the first limiting ring 152 and the second limiting ring 151. The indicating component includes a U-shaped rod 29, a positioning column 30, a first abutting ring 301, and a second abutting ring 302. A positioning column 30 is fixedly arranged at one end of the one-word rod 810 far away from the energy-saving motor 8. A U-shaped rod 29 is fixedly arranged at the end of the horizontal cylinder 28. The positioning column 30 passes through the center position of the U-shaped rod 29. The first abutting ring 301 and the second abutting ring 302 are sequentially and fixedly arranged on both side surfaces of the positioning column 30 with respect to the U-shaped rod 29. A transparent cover 6 is fixedly arranged on the outside of the right fixing ring 5 by bolts. A central hole 61 is opened at the center position of the side surface of the transparent cover 6. The diameter of the central hole 61 needs to be set larger to avoid affecting the rotation of the driving rod 23. The setting of the transparent cover 6 facilitates the user to observe the operating state inside the peristaltic pump housing.

[0049] It should be added that, in order to facilitate the quick disassembly and assembly of the horizontal cylinder 28 and all the structures installed on the horizontal cylinder 28, the second abutting ring 302 can be fixedly connected to the outer wall of the positioning column 30 by bolts. When it is necessary to separate the horizontal cylinder 28 and all the structures installed on the horizontal cylinder 28 from the one-word rod 810, the second abutting ring 302 can be disassembled from the outer wall of the positioning column 30. At this time, the horizontal cylinder 28 and all the structures installed on the horizontal cylinder 28 can be pulled out from the outer wall of the one-word rod 810.

[0050] The specific implementation steps and principles of the present invention are as follows:

[0051] When the entire peristaltic pump is working normally, the limiting block 16 is in the gap between the end of the limiting cylinder 15 and the first limiting ring 152, and the side surface of the U-shaped rod 29 is attached to the side surface of the first abutting ring 301. At this time, the horizontal cylinder 28 cannot move along the axial direction of the one-word rod 810, and the main wheel 22 is attached to the inner wall of the pump tube 53. The energy-saving motor 8 drives the main wheels 22 at both ends of the first connecting column 20 to be attached to the inner wall of the pump tube 53 through the extension column 81, the one-word rod 810, and the horizontal cylinder 28.

[0052] When the user observes that the peristaltic flow rate of the pump tube 53 significantly decreases, stop the energy-saving motor 8. Hold the U-shaped rod 29 manually so that the rectangular plate 27 cannot rotate. Manually rotate the auxiliary nut 26 counterclockwise. The auxiliary nut 26 drives the first contact post 20 and the second contact post 21, which are an integral structure, to move closer to each other by driving the push cylinder 24 and the drive rod 23. At this time, the main wheels 22 at both ends of the first contact post 20 leave the inner wall of the pump tube 53 until the guide post 19 moves to the limit position of the guide groove 271. It is difficult to rotate the auxiliary nut 26. The limit block 16 disengages from the gap between the end of the limit cylinder 15 and the first limit ring 152. The horizontal cylinder 28 can move along the axis direction of the one-word rod 810. Manually pull the U-shaped rod 29 until the U-shaped rod 29 fits against the side of the second abutting ring 302. At this time, the limit block 16 moves to the gap position between the first limit ring 152 and the second limit ring 151. The replacement wheel 220 moves into the inner circle of the pump tube 53. Finally, rotate the auxiliary nut 26 in the reverse direction. By driving the push cylinder 24 and the drive rod 23, drive the first contact post 20 and the second contact post 21, which are an integral structure, to move away from each other. At this time, the replacement wheels 220 at both ends of the second contact post 21 fit against and hold the inner wall of the pump tube 53.

[0053] The above is only a preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution and inventive concept of the present invention, makes equivalent substitutions or changes, and should be covered within the protection scope of the present invention.

Claims

1. A peristaltic pump for ultrafiltration of varicella zoster virus liquid, comprising an energy-saving motor, a main wheel, a substitute wheel, a vertical seat, and a pump tube, characterized in that, An extension cylinder is fixedly arranged on the rear side surface of the vertical seat. The energy-saving motor is fixedly connected to the side surface of the extension cylinder. A limiting component for fixedly limiting the pump pipe is arranged on the opposite side of the vertical seat to the energy-saving motor. The limiting component includes a fixing ring, a left fixing ring, a right fixing ring and a quick-fixing component. The quick-fixing component realizes the quick disassembly and assembly between the left fixing ring and the right fixing ring. The left fixing ring is fixedly arranged on the side surface of the vertical seat. Two fixing rings are fixedly arranged on the side surface of the vertical seat along the vertical direction on one side of the left fixing ring. A rectangular groove is formed in the middle position of the side surface of the left fixing ring. A rectangular column is fixedly arranged on the side surface of the right fixing ring. The rectangular column is movably inserted into the rectangular groove. A left fixing groove is formed in the side surface of the left fixing ring. A right fixing groove is formed in the side surface of the right fixing ring. The quick-fixing component includes a threaded column, a locking grip and a quick-fixing block. One end of the threaded column is fixedly connected to the side surface of the vertical seat. The quick-fixing block is movably inserted outside the threaded column. The locking grip is screwed onto the end of the outer wall of the threaded column. A limiting groove is formed in the side surface of the quick-fixing block. The total width of the limiting groove is greater than the total thickness of the vertical seat, the left fixing ring and the right fixing ring; An extension column is fixedly arranged at the output end of the energy-saving motor. A cross bar is fixedly arranged at the end of the extension column. The cross bar is movably inserted into a horizontal cylinder along the horizontal direction. A rectangular plate is fixedly arranged on the outer wall of the horizontal cylinder. Guide grooves are symmetrically formed in the rectangular plate along the vertical direction. Guide posts are movably inserted into the guide grooves. A first connecting post and a second connecting post are sequentially fixedly arranged on the outer wall of the guide post along the horizontal direction. A main wheel is rotatably connected to the end of the first connecting post. A backup wheel is rotatably connected to the end of the second connecting post. A switching component for switching the main wheel or the backup wheel to the peristaltic working position is arranged on the side surface of the second connecting post. The switching component includes a pushing cylinder, a driving rod, a limiting component and an indicating component. The limiting component is used for limiting the main wheel or the backup wheel to the peristaltic working position. The indicating component indicates that the main wheel or the backup wheel is in the peristaltic working position.

2. The peristaltic pump for ultrafiltration of varicella-zoster virus liquid according to claim 1, wherein, The pushing cylinder is screwed onto the outer wall of the horizontal cylinder. An auxiliary nut is fixedly arranged at the end of the outer wall of the pushing cylinder. A receiving groove is formed in the outer wall of the pushing cylinder. A driven ring is rotatably connected inside the receiving groove. Driving rods are symmetrically rotatably connected to the outer wall of the driven ring. The other end of each driving rod is rotatably connected to the side surface of the second connecting post.

3. A peristaltic pump for ultrafiltration of varicella-zoster virus liquid according to claim 1, characterized in that, The limiting component includes a limiting cylinder, a first limiting ring, a second limiting ring and a limiting block. A triangular plate is fixedly arranged at one end of the guide post. An extension rod is fixedly arranged at the other end of the triangular plate. The limiting block is fixedly arranged at the other end of the extension rod. The limiting cylinder is concentrically fixedly arranged on the side surface of the extension cylinder.

4. A peristaltic pump for ultrafiltration of varicella-zoster virus liquid according to claim 3, characterized in that, A first limiting ring and a second limiting ring are sequentially fixedly arranged inside the limiting cylinder. The distance between the end of the limiting cylinder and the first limiting ring is equal to the distance between the first limiting ring and the second limiting ring. The limiting block extends into the inner cavity of the limiting cylinder. The width of the limiting block is equal to the distance between the first limiting ring and the second limiting ring.

5. A peristaltic pump for ultrafiltration of varicella-zoster virus solution according to claim 1, wherein, The indicating component includes a U-shaped rod, a positioning post, a first abutting ring, and a second abutting ring. A positioning post is fixedly arranged at one end of the linear rod away from the energy-saving motor. A U-shaped rod is fixedly arranged at the end of the horizontal cylinder. The positioning post passes through the center position of the U-shaped rod. The first abutting ring and the second abutting ring are successively and fixedly arranged on both side surfaces of the U-shaped rod with respect to the positioning post.

6. A peristaltic pump for ultrafiltration of varicella zoster virus solution according to claim 1, characterized in that, A transparent cover is fixedly arranged on the outside of the right fixing ring by bolts, and a central hole is formed at the center position of the side surface of the transparent cover.

7. A peristaltic pump for ultrafiltration of varicella-zoster virus liquid according to claim 1, characterized in that, Rear support frames are symmetrically and fixedly arranged on the rear side surface of the vertical seat. A support seat is fixedly arranged at the bottom between the two rear support frames, and the top of the support seat is fixedly arranged at the bottom of the energy-saving motor.

8. An ultrafiltration process for varicella-zoster virus liquid, which is used for the peristaltic pump for ultrafiltration of varicella-zoster virus liquid according to any one of claims 1-7, characterized in that, It includes the following steps: S1, feeding stage: Store the varicella-zoster virus liquid in a dedicated sterile storage tank to ensure that the virus liquid is stored under low-temperature and constant conditions to maintain its activity. Quantitatively transport the virus liquid through this peristaltic pump so that it enters the ultrafiltration module. Set the rotation speed parameter of the peristaltic pump to control the feeding flow rate, so that the virus liquid enters the ultrafiltration system stably and evenly, thereby ensuring the continuity and stability of the filtration process and avoiding affecting the filtration efficiency and the integrity of virus particles due to flow rate fluctuations. S2, ultrafiltration stage: The ultrafiltration module uses a hollow fiber ultrafiltration membrane as the core filtration medium. By adjusting the ultrafiltration pressure and the transmembrane flow rate, the virus liquid forms a tangential flow on the membrane surface. During this process, the flow rate, pressure, and temperature of the filtrate are monitored in real time to ensure the stability of the ultrafiltration process, and the operation parameters are adjusted in a timely manner according to the actual situation to ensure the acquisition of a virus liquid with high purity and high concentration.

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