Insect repellent

Abstract

The invention discloses an insect repellent. The insect repellent provided by the invention comprises anthranilate and deet. The invention also provides an insect repellent spray which comprises the insect repellent, an organic solvent and water. The insect repellent provided by the invention is applied to preparation of products for repelling pests. The mosquito and tick repellent is a mixed preparation of anthranilate and deet, and can play a role in enhancing the effect of repelling mosquitoes and ticks; the preparation can be applied to environments with high mosquito and tick density, such as humid and hot forests, lakesides, grassland, marshes and the like with dense vegetation, is suitable for outdoor mountain climbing, exploration, fishing, picnic and field farming activities, can be sprayed to skin or clothes, can effectively prevent various mosquito and tick bites, and has a good application prospect. Comprise aedes aegypti, aedes inside, aedes albopictus, culex pipicorus, culex modestus, haemaphysalis unguiculata, picetophysalis katsumadai and the like.

Description

Technical Field

[0001] The present invention belongs to the field of pest control and hygiene, and relates to an insect repellent. Background Art

[0002] Sanitary pests can endanger human life in various ways such as harassment, biting, and parasitism, and can spread pathogens to cause the outbreak of epidemics such as plague, typhoid, malaria, and encephalitis, seriously threatening people's lives.

[0003] There are a large number of existing mosquito repellent products. Most of the ingredients for repelling insects mainly include plant-derived ingredients and chemically synthesized agents. Plant-derived ingredients such as lemon eucalyptus oil, citronellol, and mint, although sourced from natural environments and relatively safe for human health, have a relatively short effective mosquito repellent time and have no repellent effect on ticks. Insect repellents are active chemical substances that are produced by plants or synthesized artificially and have the effect of repelling insects. The insect repellent with the patent publication number CN 105744832 B has methyl anthranilate and fatty acids as its active ingredients; in Examples 17 and 24, the methyl anthranilate and fatty acid preparation repels Aedes aegypti. After applying the preparation to a membrane feeder containing a blood meal, it can repel the mosquito's blood-sucking mode feeder; in Example 16, the methyl anthranilate and fatty acid preparation can repel the brown dog tick; however, the insect repellent among them has not been actually verified in the field environment. Repellents are agents that can make pests flee. Although these agents themselves have no toxic effect on killing pests, due to their special smell, they can make pests avoid or disperse. Chemical components such as diethyltoluamide (DEET), ethyl hexanediol 3-(N-butyl-N-acetyl) aminopropionate (IR3535), picaridin, and pyrethrins, the effective time of repellents is relatively long, but a single-component repellent cannot resist mosquito bites at a high density. For example, diethyltoluamide (DEET), a traditional mosquito repellent ingredient, is widely used in various mosquito repellent products. Most of the common effective mosquito repellent waters on the market contain DEET. Spraying it on the skin or clothes can avoid mosquito bites. The patent (CN 102920617 B) discloses an aqueous DEET composition and its preparation, proving that DEET can effectively repel Aedes albopictus and has no irritating reaction to the skin of New Zealand rabbits. The patent (CN102396572A) discloses a method for making an aerosol mosquito repellent with 10-20% DEET, 10-20% citronella oil, 10-20% polyethylene glycol, and 50-80% inert gas. Such substances are convenient to use and have an ideal effect, but the cost is relatively high. The mosquito repellent solution is made into an oil-based type for smearing to prevent mosquitoes. For example, the patent (CN1391800A) discloses a method for making a mosquito repellent using zinc stearate, magnesium stearate, glycerol, polyethylene glycol, and water; however, the effects of these mosquito repellents have only been verified in the laboratory, and the repellent effects in the wild with a high mosquito density are unknown. Methyl anthranilate esters, including methyl anthranilate, ethyl anthranilate, and butyl anthranilate; methyl anthranilate and ethyl anthranilate are light yellow liquids with a grape-like smell and are approved for use as food additives. Currently, there is no effective repellent product for repelling ticks.

[0004] Therefore, for extreme environments with high densities of mosquitoes and ticks and epidemic areas of mosquito- and tick-borne diseases, a powerful and effective repellent is needed to achieve the effect of repelling insects. Summary of the Invention

[0005] The object of the present invention is to provide an insect repellent.

[0006] The present invention designs a pest repellent composition containing DEET and o-aminobenzoates for mosquitoes and ticks.

[0007] The present invention provides an insect repellent, comprising an o-aminobenzoate and DEET.

[0008] In the above-mentioned insect repellent, the volume ratio of the o-aminobenzoate to the DEET is 1:1 to 5.

[0009] In the above-mentioned insect repellent, the o-aminobenzoate is selected from at least one of methyl o-aminobenzoate, ethyl o-aminobenzoate, and butyl o-aminobenzoate.

[0010] The present invention also provides an insect repellent spray, comprising the above-mentioned insect repellent, an organic solvent, and water.

[0011] In the above-mentioned insect repellent spray, the organic solvent is selected from at least one of propylene glycol, ethanol, ethylene glycol, and glycerol, preferably a mixed solution of propylene glycol and ethanol, more preferably propylene glycol and ethanol with a volume ratio of 3:40. Other solvents may cause the insect repellent spray to layer.

[0012] In the above-mentioned insect repellent spray, the insect repellent spray comprises the following components in volume percentage: based on a total volume of 100%:

[0013] o-aminobenzoate 5% - 20%;

[0014] DEET 5% - 20%;

[0015] organic solvent 30% - 45%;

[0016] the balance is water.

[0017] In the above-mentioned insect repellent spray, the insect repellent spray is composed of the following components in volume percentage: DEET 10 - 20%, methyl o-aminobenzoate 20%, propylene glycol 3%, ethanol 40%, and the balance is water.

[0018] In the present invention, (1) the insect repellent spray is specifically composed of the following components in volume percentage: DEET 20%, methyl o-aminobenzoate 20%, propylene glycol 3%, ethanol 40%, and water 37%;

[0019] (2) the insect repellent spray is specifically composed of the following components in volume percentage: DEET 10%, methyl o-aminobenzoate 10%, propylene glycol 3%, ethanol 40%, and water 27%.

[0020] The insect repellent described in the present invention is applied to the preparation of products for repelling pests.

[0021] In the above application, the pest expulsion includes Diptera or ticks;

[0022] In the above application, the pest expulsion includes at least one of Aedes aegypti, Aedes caspicus, Aedes vexans, Culex pipiens, Culex modestus, Haemaphysalis longicornis, and Rhipicephalus turanicus.

[0023] The insect repellent spray described in the present invention is applied to the preparation of products for expelling pests.

[0024] In the above application, the pest expulsion includes Diptera or ticks.

[0025] In the above application, the pest expulsion includes at least one of Aedes aegypti, Aedes caspicus, Aedes vexans, Culex pipiens, Culex modestus, Haemaphysalis longicornis, and Rhipicephalus turanicus.

[0026] The present invention has the following beneficial effects:

[0027] 1. The phthalate esters are food and cosmetic additives, colorless oily liquids or white crystals, with the aroma and sweetness of neroli and glucose; another active ingredient is DEET, a widely used mosquito repellent with low toxicity. The mixed preparation of phthalate esters and DEET can enhance the effect of expelling mosquitoes and ticks.

[0028] 2. The mixed preparation of phthalate esters and DEET can be applied to environments with a high density of mosquitoes and ticks, such as densely vegetated, humid and hot forests, lakesides, grasslands, swamps, etc., and is suitable for outdoor activities such as mountain climbing, exploration, fishing, picnicking, and field farming. The preparation can be sprayed on the skin or clothing to effectively prevent bites from various mosquitoes and ticks, including Aedes aegypti, Aedes caspicus, Aedes vexans, Culex pipiens, Culex modestus, Haemaphysalis longicornis, and Rhipicephalus turanicus, etc. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 It shows the effect of expelling Aedes aegypti in the laboratory in Example 1 of the present invention.

[0030] Figure 2 It shows the effect of expelling Haemaphysalis longicornis in the laboratory.

[0031] Figure 3 It shows the effect of expelling mosquitoes in Nanhe.

[0032] Figure 4 It shows the effect of expelling mosquitoes in Beiwan.

[0033] Figure 5 It shows the effect of expelling Aedes aegypti in the laboratory in Example 2 of the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0034] Unless otherwise specified, the experimental methods used in the following examples are all conventional methods.

[0035] Unless otherwise specified, the materials, reagents, etc. used in the following examples are all commercially available.

[0036] Example 1

[0037] (I) Repellent formulation

[0038] Formulation A (as a control): Prepare the following formulation (volume %) for testing the insect repellency rate: Mix 20% DEET, 3% propylene glycol, 40% ethanol, and 37% water until homogeneous, and fill it into a spray bottle.

[0039] Formulation B (experimental group): Prepare the following formulation (volume %) for testing the insect repellency rate: Mix 20% DEET, 20% methyl anthranilate, 3% propylene glycol, 40% ethanol, and 17% water until homogeneous, and fill it into a spray bottle.

[0040] Formulation C (as a control): Prepare the following formulation (volume %) for testing the insect repellency rate: Mix 20% methyl anthranilate, 3% propylene glycol, 40% ethanol, and 37% water until homogeneous, and fill it into a spray bottle.

[0041] (II) Repellent against Aedes aegypti

[0042] Prepare 4 colorless and transparent mosquito cages with a height of 170 mm and an inner diameter of 120 mm. Put 40 unfed female Aedes aegypti on the 5th day after eclosion in each mosquito cage, and place them in an incubator at 28 °C for 5 h to allow the mosquitoes to recover their vitality before the test. ICR mice, 4 - 6 weeks old. Shave the abdominal hair of the ICR mice clean, and evenly spray the repellent formulations A, B, and C (150 μl) on the abdomen and limbs of each mouse. Spray an equal amount of distilled water on the control group, and put them back into the mouse cage; Fix the mice sprayed with the repellent and distilled water on the mosquito cage respectively, and align their abdomen with the mosquito cage net so that the mosquito mouthparts can touch the mouse skin, and observe whether the mosquitoes suck blood; Record the full blood-sucking rate of the mosquitoes. As Figure 1 shown, formulations A, B, and C all have the repellent effect on Aedes aegypti. Formulation A (DEET) as a positive control can achieve complete protection for 4 hours. After 5 hours of formulation C (methyl anthranilate), only 11 mosquitoes sucked blood, while 40 mosquitoes sucked blood in the control group. Formulation B (DEET + methyl anthranilate) can achieve complete protection for 5 hours. It shows that the formulation B of the present invention has a very excellent repellent effect on Aedes aegypti.

[0043] (III) Repellent against Haemaphysalis longicornis

[0044] Prepare adult female Haemaphysalis longicornis ticks that have molted for 3 months for the repellent experiment. Use ICR mice, 4 - 6 weeks old. Shave the abdominal hair of the ICR mice clean, and evenly spray repellent preparations A, B, and C (150 μl) on the abdomen and limbs of each mouse. Spray an equal amount of distilled water on the control group, and then place the mice back into the cages. Fix the mice sprayed with the repellents and distilled water on the mouse racks respectively, place the mouse racks in transparent plastic boxes with a length of 200 mm, a width of 300 mm, and a height of 300 mm. Place 30 hungry ticks on the backs of the mice and observe whether the ticks suck blood. Every 1 h, put the ticks scattered in the box back onto the backs of the mice and record the number of ticks that bite the mice. As Figure 2 shown, preparations A, B, and C all have the effect of repelling Haemaphysalis longicornis. Preparation A (DEET) is used as a positive control and can achieve complete protection for 5 hours. After 3 hours of preparation C (methyl anthranilate), only 1 Haemaphysalis longicornis tick sucks blood, while 26 Haemaphysalis longicornis ticks suck blood in the control group. After 5 hours of preparation B (DEET + methyl anthranilate), only 4 Haemaphysalis longicornis ticks suck blood, while 27 Haemaphysalis longicornis ticks suck blood in the control group. By using preparation A as a positive control, preparation B of the present invention has a very excellent repellent effect on Haemaphysalis longicornis.

[0045] (IV) Repellents repel Aedes aegypti in the field environment

[0046] Test sites: South River Birch Forest and the forest grassland by the pond in Beihai, Regiment 185, Xinjiang Production and Construction Corps. The mosquito density in these two areas is as high as 1000 mosquitoes / cm 2 , and the mosquitoes collected are mainly Aedes stimulans, Aedes caspius, Culex pipiens pipiens, and Culex modestus. There is 1 person in the control group (not spraying repellent), and 4 - 5 people in the experimental group (spraying repellent preparation B). The testers cannot use any cosmetics, including perfume, cologne, or lotion on the day of the test and the day before. The subjects wash their legs with soap after the test and monitor their health conditions. Test process: Expose one calf of the experimental group, spray 1 g of repellent preparation B, and cover the remaining parts completely to prevent being bitten. Conduct the detection 15 minutes after application. Every 30 minutes, the testers are transferred to a new location, at least 10 meters away from the previous location. All testers sit on chairs, at least 10 meters apart, with their legs exposed for 5 minutes. Use a mosquito suction device to collect the mosquitoes that land on and bite them, try to prevent being bitten, and count the number of mosquitoes. Continuously monitor for 0 to 5 hours. When the test site is in the South River of Regiment 185, Xinjiang Production and Construction Corps, after 5 hours, 280 mosquitoes are collected by the control group within 5 minutes, while the experimental group collects an average of 10 mosquitoes, and the repellent effect is greater than 96%. When the test site is in Beihai, Xinjiang, after 5 hours, 108 mosquitoes are collected by the control group within 5 minutes, while the experimental group collects an average of 4 mosquitoes, and the repellent effect is greater than 96%. As Figure 3 and Figure 4As shown, in the outdoor environment with a large number of mosquitoes, when the leg is sprayed with the repellent preparation B, the repellent effect can reach at least 5 hours, indicating that the preparation B of the present invention has an excellent repellent effect on outdoor mosquitoes.

[0047] Example 2

[0048] Preparation D (as a control): Prepare the following preparation (volume %) for testing the insect repellent rate: Mix 10% DEET, 3% propylene glycol, 40% ethanol, and 47% water until homogeneous, and fill it in a spray bottle.

[0049] Preparation E (experimental group): Prepare the following preparation (volume %) for testing the insect repellent rate: Mix 10% DEET, 20% methyl anthranilate, 3% propylene glycol, 40% ethanol, and 27% water until homogeneous, and fill it in a spray bottle.

[0050] Preparation C (as a control): Prepare the following preparation (volume %) for testing the insect repellent rate: Mix 20% methyl anthranilate, 3% propylene glycol, 40% ethanol, and 37% water until homogeneous, and fill it in a spray bottle.

[0051] Prepare 4 colorless and transparent mosquito cages with a height of 170 mm and an inner diameter of 120 mm. Put 40 non-blood-sucking female Aedes aegypti on the 5th day after eclosion in each mosquito cage, and place them in an incubator at 28 °C for 5 h to let the mosquitoes recover their vitality before the experiment. ICR mice, 4 - 6 weeks old. Remove the abdominal hair of the ICR mice completely, and evenly spray the repellent preparations D, E, and C (150 μl) on the abdomen and limbs of each mouse. Spray an equal amount of distilled water on the control group, and put them back into the mouse cages; Fix the mice sprayed with the repellent and distilled water on the mosquito cages respectively, and align their abdomens with the mosquito net of the mosquito cage so that the mosquito mouthparts can touch the mouse skin, and observe whether the mosquitoes suck blood; Record the blood-satiation rate of the mosquitoes. As Figure 5 shown, preparations D, E, and C all have the repellent effect on Aedes aegypti. Preparation E (10% DEET + 20% methyl anthranilate) has a better repellent protection effect than using preparation D (10% DEET) or preparation C (20% methyl anthranilate) alone. It shows that the preparation E of the present invention has a very excellent repellent effect on Aedes aegypti.

Claims

1. An insect repellent composition, characterized in that, it comprises o-aminobenzoate and DEET.

2. The insect repellent composition according to claim 1, characterized in that, the volume ratio of the o-aminobenzoate to the DEET is 1:1 to 5.

3. The insect repellent composition according to claim 1 or 2, characterized in that, the o-aminobenzoate is selected from at least one of methyl o-aminobenzoate, ethyl o-aminobenzoate and butyl o-aminobenzoate.

4. An insect repellent spray, characterized in that, it comprises the insect repellent composition according to any one of claims 1-3, an organic solvent and water.

5. The insect repellent spray according to claim 4, characterized in that, the organic solvent is selected from at least one of propylene glycol, ethanol, ethylene glycol and glycerol.

6. The insect repellent spray according to claim 4 or 5, characterized in that, the insect repellent spray comprises the following components in volume percentage: based on the total volume of 100%, o-aminobenzoate 5% - 20%; DEET 5% - 20%; organic solvent 30% - 45%; the balance is water.

7. The insect repellent spray according to claim 6, characterized in that, the insect repellent spray consists of the following components in volume percentage: DEET 10 - 20%, methyl o-aminobenzoate 20%, propylene glycol 3%, ethanol 40%, and the balance is water.

8. Use of the insect repellent composition according to any one of claims 1-3 or the insect repellent spray according to any one of claims 4-7 in the preparation of a product for repelling pests.

9. The use according to claim 8, characterized in that, the pest repelling includes Diptera or ticks.

10. The use according to claim 9, characterized in that, the pest repelling includes at least one of Aedes aegypti, Aedes caspicus, Aedes vexans, Culex pipiens, Culex modestus, Haemaphysalis longicornis and Rhipicephalus turanicus.

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