Bacterial inhibitor composition and application thereof

Abstract

The invention relates to a bacterial inhibitor composition and application thereof. The active ingredients of the composition comprise malononitrile compounds and copper hydroxide. According to the composition disclosed by the invention, the malononitrile compound FM-86 and the copper hydroxide are compounded to generate a synergistic effect, so that the dosage of pesticides is reduced, and the pesticide cost and the residual quantity of the pesticides on crops are reduced. Meanwhile, the malononitrile compound and the copper hydroxide have different action mechanisms, and the malononitrile compound and the copper hydroxide are compounded for use, so that the resistance generation of the bacteriostatic agent can be effectively delayed, and the service life of the pesticide variety is prolonged.

Description

Technical Field

[0001] The invention belongs to the field of bacterial inhibitors, and in particular relates to a bacterial inhibitor composition and application thereof. Background Art

[0002] Chinese invention patent CN202010062721.0 and PCT application WOCN111484429 disclose a malononitrile compound, which also has a good control effect on plant pathogenic bacteria. FM-86 belongs to the malononitrile compound, and its chemical structure is shown in Formula I. It is safe, efficient, low-toxic, green and environmentally friendly, has a good antibacterial effect on citrus canker, and has excellent control effect on citrus canker in the field.

[0003]

[0004] Copper hydroxide is a broad-spectrum protective fungicide that can be used to prevent and treat bacterial and fungal diseases of a variety of crops. Its fungicidal mechanism is to release copper ions, destroy the cell wall and membrane of pathogens, interfere with their metabolism and respiration, and thus inhibit their growth and reproduction. Agricultural copper hydroxide is mainly used as a fungicide to treat diseases such as citrus canker and anthracnose. Copper hydroxide is not easy to develop drug resistance and can be mixed with other fungicides to increase the prevention and control effect. It also has a certain slow-release effect and can form a lasting protective film on the surface of crops to prolong the efficacy of the drug. It can also reduce the effect of frost and improve the cold resistance of crops. It is also less toxic to the environment and humans and animals, and is safe and reliable.

[0005] Reasonable fungicidal compositions have important functions such as expanding fungicidal spectrum, improving control effect, extending application period, reducing dosage, reducing pesticide damage, reducing residue, delaying the occurrence and development of drug resistance, etc. Therefore, in the art, it is expected to develop reasonable fungicidal compositions to solve the above problems and meet the needs of agriculture. Summary of the invention

[0006] Problem that the invention aims to solve

[0007] Based on the above problems existing in the prior art, the object of the present invention is to provide a bacterial inhibitor composition containing a malononitrile compound FM86 and copper hydroxide, while enhancing its control effect and expanding its application range.

[0008] Solutions for solving problems

[0009] The invention provides a bacteria inhibitor composition, wherein the active ingredients of the bacteria inhibitor composition include: a malononitrile compound and copper hydroxide.

[0010] Preferably, the structure of the malononitrile compound is as shown in Formula I:

[0011]

[0012] Preferably, the weight ratio of the malononitrile compound to copper hydroxide is 1:100 - 100:1; more preferably, the weight ratio of the malononitrile compound to copper hydroxide is 1:50 - 50:1; further preferably, the weight ratio of the malononitrile compound to copper hydroxide is 1:10 - 10:1; most preferably, the weight ratio of the malononitrile compound to copper hydroxide is 1:1 - 1:3.

[0013] In certain embodiments, the weight ratio of the malononitrile compound to copper hydroxide is 1:100 - 100:1.

[0014] In certain embodiments, the weight ratio of the malononitrile compound to copper hydroxide is 1:50 - 50:1.

[0015] In certain embodiments, the weight ratio of the malononitrile compound to copper hydroxide is 1:10 - 10:1.

[0016] In certain embodiments, the weight ratio of the malononitrile compound to copper hydroxide is 1:1 - 1:3.

[0017] In certain embodiments, the weight ratio of the malononitrile compound to copper hydroxide is 1:1, or 1:2, or 1:3.

[0018] The bacterial inhibitor composition can be prepared into any agriculturally acceptable dosage form; more preferably, the dosage form includes emulsifiable concentrate, microemulsion, aqueous emulsion, suspension concentrate, oil-dispersible suspension, wettable powder, emulsifiable powder, granule, water-dispersible granule, emulsifiable granule, dry flowable, tablet, effervescent tablet; further preferably, the dosage form is wettable powder, or water-dispersible granule, or suspension concentrate.

[0019] Preferably, the bacterial inhibitor composition formulation further includes at least one auxiliary and / or at least one carrier; more preferably, the active ingredient in the bacterial inhibitor composition accounts for 0.5% - 95% of the total weight; preferably 1% - 90%. In certain embodiments, according to different formulation types, the content of the active component is different. Generally, the content of the active ingredient in the liquid formulation accounts for 1% - 80% of the total weight, preferably 5% - 50%; the content of the active ingredient in the solid formulation accounts for 1% - 85% of the total weight, preferably 5% - 80%.

[0020] In certain embodiments, the weight percentage content of the active ingredient is 16% - 40% of the composition.

[0021] In certain embodiments, the weight percentage content of the active ingredient is 16%, or 20%, or 24%, or 30%, or 40% of the composition.

[0022] In certain embodiments, the weight percentage content of the active ingredient is 30% of the composition.

[0023] In certain embodiments, the preparation can be processed into any pesticide-acceptable dosage form as needed, and the more preferred dosage forms are: emulsifiable concentrate, microemulsion, aqueous emulsion, suspension concentrate, oil-dispersible suspension, wettable powder, emulsifiable powder, granule, water-dispersible granule, emulsifiable granule, dry flowable, tablet, effervescent tablet.

[0024] The bacterial inhibitor composition of the present invention can be prepared by conventional processing methods known to those skilled in the art, that is, after mixing the active substance with a liquid carrier or a solid carrier, one or several surfactants such as emulsifiers, dispersants, wetting agents, thickeners, stabilizers and defoamers are added. Usually, the composition contains at least one carrier and at least one surfactant. In all cases, it should be ensured that the active components of the composition of the present invention are evenly distributed.

[0025] The emulsifiable concentrate is prepared by uniformly mixing FM86, copper hydroxide, surfactant, and organic solvent to form a homogeneous oily liquid. The emulsifiable concentrate usually contains 1-70% of FM86, 1-70% of copper hydroxide, 5-20% of emulsifier, 0-20% of other additives such as penetrant, stabilizer, etc., and the balance of the liquid carrier.

[0026] The microemulsion is prepared by uniformly mixing FM86, copper hydroxide, surfactant, water, and organic solvent to form a homogeneous transparent liquid. The microemulsion usually contains 1-50% of FM86, 1-50% of copper hydroxide, 5-30% of emulsifier, 2-10% of antifreeze, 0-20% of other additives such as penetrant, stabilizer, etc., and the balance of the liquid carrier.

[0027] The aqueous emulsion is prepared by uniformly mixing FM86, copper hydroxide, surfactant, and organic solvent to form an oil phase; water and antifreeze are mixed together to form a water phase. The oil phase is sheared at high speed with a high-shear emulsifier, and the water phase is slowly added to the oil phase at the same time to obtain a uniformly dispersed aqueous emulsion. It usually contains 1-50% of FM86, 1-50% of copper hydroxide, 5-20% of emulsifier, 2-5% of antifreeze, 0-20% of other additives such as thickener, preservative, stabilizer, etc., and the balance of the liquid carrier.

[0028] The described suspending agent is prepared by uniformly mixing FM86, copper hydroxide, dispersant, wetting agent, antifreeze, and water, and then grinding it with a sand mill to obtain a stable, non-depositing, and flowable liquid. The suspending agent usually contains 1-50% of FM86, 1-50% of copper hydroxide, 2-10% of dispersant, 2-10% of wetting agent, 2-5% of antifreeze, 0-20% of other additives such as defoaming agent, thickening agent, preservative, etc., and the balance is the liquid carrier.

[0029] The described dispersible oil suspending agent is prepared by uniformly mixing FM86, copper hydroxide, surfactant, and oil-based carrier, and then grinding it with a sand mill to obtain a stable, non-depositing, and flowable liquid. The dispersible oil suspending agent usually contains 1-50% of FM86, 1-50% of copper hydroxide, 5-30% of surfactant, 0-20% of other additives such as thickening agent, stabilizer, and the balance is the liquid carrier.

[0030] The described wettable powder is prepared by mixing FM86, copper hydroxide, dispersant, wetting agent, and carrier, and then grinding to obtain a powder. The wettable powder usually contains 1-80% of FM86, 1-80% of copper hydroxide, 1-10% of dispersant, 1-10% of wetting agent, 0-20% of other additives such as defoaming agent, stabilizer, etc., and the balance is the solid carrier.

[0031] The described emulsifiable powder is prepared by mixing FM86, copper hydroxide, surfactant, and organic solvent to form a homogeneous transparent oil phase, and then uniformly spraying the above oil phase on the pre-ground carrier. The emulsifiable powder usually contains 1-50% of FM86, 1-50% of copper hydroxide, 5-30% of emulsifier, 5-20% of wetting dispersant, 0-30% of organic solvent, 0-20% of other additives such as defoaming agent, stabilizer, etc., and the balance is the solid carrier.

[0032] The described granule is prepared by mixing FM86, copper hydroxide, auxiliary agent, and carrier, and then kneading, granulating, drying, and coating. The granule usually contains 0.5-20% of FM86, 0.5-20% of copper hydroxide, 0.1-10% of binder, 0-10% of other additives such as stabilizer, defoaming agent, etc., and the balance is the solid carrier.

[0033] The described water dispersible granule is prepared by mixing FM86, copper hydroxide, dispersant, wetting agent, disintegrant, and carrier, and then kneading, granulating, and drying. The water dispersible granule usually contains 1-80% of FM86, 1-80% of copper hydroxide, 1-10% of dispersant, 1-10% of wetting agent, 0.1-10% of binder, 0.1-10% of disintegrant, 0-10% of other additives such as defoaming agent, stabilizer, etc., and the balance is the solid carrier.

[0034] The described emulsifiable granule is prepared by mixing FM86, copper hydroxide, surfactant, and organic solvent to form a homogeneous and transparent oil phase, and then uniformly spraying the above oil phase onto a pre-crushed carrier, followed by kneading, granulating, and drying. The emulsifiable granule usually contains 1 - 50% of FM86, 1 - 50% of copper hydroxide, 5 - 30% of emulsifier, 5 - 10% of wetting and dispersing agent, 0 - 15% of organic solvent, 0 - 20% of other additives such as disintegrant, stabilizer, binder, defoamer, etc., and the balance is the solid carrier.

[0035] The described dry suspension is prepared by uniformly mixing FM86, copper hydroxide, dispersant, wetting agent, carrier, and water, followed by sand milling with a sand mill, and then spray drying after sand milling. The dry suspension usually contains 1 - 80% of FM86, 1 - 80% of copper hydroxide, 2 - 20% of dispersant, 2 - 10% of wetting agent, 0 - 20% of other additives such as defoamer, thickener, etc., and the balance is the solid carrier.

[0036] The described tablet is prepared by mixing FM86, copper hydroxide, dispersant, wetting agent, disintegrant, and carrier, followed by kneading, tabletting, and drying. The tablet usually contains 1 - 30% of FM86, 1 - 30% of copper hydroxide, 1 - 10% of dispersant, 1 - 10% of wetting agent, 1 - 10% of disintegrant, 0.1 - 10% of binder, 0 - 15% of other additives such as defoamer, stabilizer, etc., and the balance is the solid carrier.

[0037] The described effervescent tablet is prepared by mixing FM86, copper hydroxide, wetting and dispersing agent, effervescent disintegrant, and carrier, followed by kneading and tabletting. The effervescent tablet usually contains 0.5 - 30% of FM86, 0.5 - 30% of copper hydroxide, 2 - 20% of wetting and dispersing agent, 2 - 20% of effervescent disintegrant, 0 - 20% of other additives such as stabilizer, binder, defoamer, etc., and the balance is the solid carrier.

[0038] The composition described in the present invention is mixed with (liquid or solid) agriculturally acceptable carriers and adjuvants known to those skilled in the art. For example, it includes but is not limited to the following various substances.

[0039] In the preparation of the bacterial inhibitor composition of the present invention, suitable surfactants can be emulsifiers, dispersants or wetting agents; they can be one or several of non-ionic or ionic types. Ionic surfactants are selected from sulfonates, sulfates, carboxylates, phosphate esters, succinate esters, lignosulfonates, acrylamide-acrylic acid copolymers, etc. Non-ionic surfactants are selected from fatty alcohol polyoxyethylene ethers, alkylphenol polyoxyethylene ethers, fatty amine polyoxyethylene ethers, fatty acid polyoxyethylene ethers, acid alcohol esters and their polyoxyethylene ethers, alkyl polyethylene glycol ethers, alkyl phenyl polyethylene glycol ethers, fatty acid amides and their polyoxyethylene ethers, alkanolamides and their polyoxyethylene ethers, polyoxyethylene-polyoxypropylene block copolymers, sodium alkyl naphthalene sulfonate fatty alcohol polyoxyethylene ether, sorbitan fatty acid ester polyoxyethylene ether, etc.

[0040] The above-mentioned surfactant can be selected from one or more of the surfactants shown, such as: sodium or calcium salt of lignin sulfonate, polyoxyethylene (n20) phenethyl phenol ether oleate, alkyl aromatic polyoxyethylene polyoxypropylene ether, tristyrylphenol polyoxyethylene (n20) ether phosphorylated triethanolamine salt, Nongru 0201B, Nongru 0203B, Nongru 100#, Nongru 600#, Nongru 700#, Nongru 1601#, Nongru AEO-3, Nongru AEO-5, Nongru AEO-7, Nongru T-20, Nongru T-80, Nongru T-85, Nongru S-80, Nongru S-85, Nongru NP -7, Nongru NP-10, Nongru NP-15, Nongru OX-2681, Nongru OX-8686, Nongru OX-690, Nongru 2201#, polycarboxylate dispersant GY-D800, polycarboxylate dispersant GY-D04, polycarboxylate dispersant GY-D02, alkyl naphthalene sulfonate formaldehyde condensate (NNO), naphthol sulfonic acid formaldehyde condensate sodium salt, alkylphenol polyoxyethylene polyoxypropylene ether, styrene maleic anhydride, methylnaphthalene sulfonic acid formaldehyde condensate, castor oil ethylene oxide adduct, alkylphenol polyoxyethylene polyoxypropylene ether, alkyl-diethylene glycol ether-sulfonate sodium, N-methyl-oleoyl Sodium taurate, detergent LS, sodium methylenenaphthalene sulfonate, sodium oleate methylaminoethyl sulfonate, dispersant SP-28F, dispersant SP-SC3, Darun dispersant D909S, alkyl aryl polyoxyethylene ether, dodecyl polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether formaldehyde condensate, sodium dibutylnaphthalene sulfonate (pulling powder BX), dibutylnaphthalene sulfonic acid formaldehyde condensate, dispersant SD-811, dispersant SD815, dispersant SK-24, dispersant SK-20TX, dispersant SK-5218, dispersant SK-33H, dispersant SK-10LX, dispersant SK -551, Dispersant Atlox4913, Dispersant EL-20, Dispersant EL-40, Dispersant EL-90, Dispersant YUS-NV1203, Dispersant YUS-NV1420, Dispersant YUS-WG4, Dispersant YUS-TG285, Dispersant YUS-WP1, Dispersant YUS-110, Dispersant YUS-EP60P, Dispersant YUS-CH1100, Dispersant SP-OF3468, Dispersant SP-OF3472, Dispersant SP-2728, Dispersant SP-SC29, Dispersant Supragil MNS / 90, dispersant Soprophor FD, dispersant YUS-FS1, dispersant YUS-PQ100, dispersant YUS-WG5, YUS-D935, octylphenol polyoxyethylene ether sulfate, Morwet EFW, wetting agent Igepal BC / 10, wetting agent GEROPONL-WET / P, wetting agent Rhodasurf860 / p, wetting agent SP-SC3266, wetting agent PICO-SW2, wetting agent PICO-SW3, wetting agent YUS-LXC, wetting agent YUS-204, sodium sulfate of alkyl alcohol polyoxyethylene ether, formaldehyde condensate sulfate of alkylphenol polyoxyethylene ether, alkyl succinic acid sulfonate, sodium dodecylbenzene sulfonate, sodium dodecyl sulfate, calcium dodecylbenzene sulfonate, etc.

[0041] The suitable liquid carrier in the preparation of the bacterial inhibitor composition of the present invention can be one or more of water, organic solvents, and oily media. Suitable organic solvents are selected from aromatic hydrocarbons, chlorinated aromatic hydrocarbons, aliphatic hydrocarbons, chlorinated aliphatic hydrocarbons, alcohols, and their ethers and esters, ketones, etc., such as benzene, xylene, toluene, alkylbenzenes, alkylnaphthalenes, chlorobenzenes, vinyl chloride, trichloroethane, dichloromethane, chloroform, carbon tetrachloride, polychlorinated ethanes, petroleum fractions, cyclohexane, methanol, ethanol, isopropanol, butanol, ethylene glycol, propylene glycol, glycerol, sorbitol, benzyl alcohol, furfuryl alcohol, cyclohexanol, acetone, methyl ethyl ketone, methyl isobutyl ketone, cyclohexanone, N-methyl-pyrrolidone, tributyl phosphate, dimethylformamide, and dimethyl sulfoxide, etc. Suitable oily media are selected from soybean oil, methyl oleate, light mineral oil, liquid paraffin, kerosene, turpentine, etc.

[0042] The solid carrier includes natural or synthetic ones. It can be selected from, but not limited to, clay, rock powder, chalk, quartz, clay, montmorillonite, sodium sulfate, silicon dioxide, diatomaceous earth, pumice, gypsum, talc, bentonite, kaolin, attapulgite, light calcium carbonate, pottery clay, montmorillonite, aluminum magnesium silicate, atavistic clay, white carbon black, ammonium sulfate, coumarone resin, superphosphate, aluminum oxide, calcite, marble, pumice, etc. Suitable particle carriers include broken and graded natural rocks such as sepiolite and dolomite and synthetic particles made of organic and inorganic powders.

[0043] The adhesive and thickener include synthetic or natural water-soluble polymers, and can be selected from, but not limited to, carboxymethyl alcohol, polyvinyl alcohol, polyvinyl acetate, xanthan gum, gelatin, gum arabic, polyvinyl pyrrolidone, magnesium aluminum silicate, polyvinyl alcohol, polyethylene glycol, phenolic resin, shellac, methyl cellulose, soluble starch, cyclodextrin, carboxymethyl cellulose and sodium alginate, and are added to the preparation in the form of powder, granules or latex.

[0044] The defoaming agent can be selected from but not limited to defoaming agent SAG1522, silicones, silicone oil, C8-10 fatty alcohols, phosphates, C10-20 saturated fatty acids (such as capric acid) and amides.

[0045] The antifreeze agent may be selected from but is not limited to ethanol, isopropanol, ethylene glycol, propylene glycol, glycerol, diethylene glycol, ethylene glycol butyl ether, propylene glycol butyl ether, ethylene glycol butyl ether acetate, urea, and the like.

[0046] The penetrant may be selected from but not limited to silicone, penetrant T, penetrant JFC, and the like.

[0047] The preservative can be selected from but not limited to sodium benzoate, kasone, potassium sorbate and the like.

[0048] The stabilizer is selected from epoxy soybean oil, epichlorohydrin, triphenyl phosphite, glycidyl ether and pentaerythritol.

[0049] The disintegrant can be selected from but not limited to sodium carboxymethyl starch, cross-linked sodium carboxymethyl starch, cross-linked sodium carboxymethyl cellulose, modified starch, cross-linked polyvinyl pyrrolidone, ammonium sulfate, sodium sulfate, sodium chloride, ammonium chloride and the like.

[0050] The effervescent disintegrant can be an acidic component and / or an alkaline component, wherein the acidic component can be selected from organic acids, inorganic acids, such as tartaric acid, citric acid, salicylic acid, phosphoric acid, etc.; the alkaline component can be selected from basic carbonates, carbonates, such as sodium bicarbonate, sodium carbonate, potassium bicarbonate, potassium carbonate, ammonium bicarbonate, etc.

[0051] The present invention also provides an application of the bacteria inhibition composition in preventing and / or treating plant bacterial diseases.

[0052] Preferably, the plant bacterial diseases include bacterial angular leaf spot of cucumber and tobacco wildfire caused by Pseudomonas species, rice white leaf blight, rice bacterial leaf streak, cucumber bacterial leaf blight, cabbage black rot, citrus canker caused by Xanthomonas species, Chinese cabbage soft rot and apricot fire blight caused by Erwinia species, tomato and peanut bacterial wilt caused by Ralstonia species, and tomato canker caused by Corynebacterium species; more preferably, the plant bacterial disease is citrus canker.

[0053] In certain embodiments, the bacterial inhibitor composition preparation can be diluted by the user or directly added to water for spraying before use, or can be used directly.

[0054] Effects of the Invention

[0055] FM-86 is a malononitrile compound with excellent antibacterial effect; copper hydroxide is a broad-spectrum protective fungicide that releases copper ions to destroy the cell walls and membranes of pathogens, interfere with their metabolism and respiration, and thus inhibit their growth and reproduction. The two active ingredients have different mechanisms of action, and their combined use can effectively delay the development of resistance to antibacterial agents.

[0056] The composition of the invention produces a synergistic effect through the compounding of FM-86 and copper hydroxide, reduces the amount of pesticide used, reduces the cost of pesticide use and the amount of pesticide residue on crops. DETAILED DESCRIPTION

[0057] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is illustrated by the following specific embodiments. The embodiments are intended to better explain the present invention rather than to limit the protection scope of the present invention. All modifications and changes made based on the basic ideas and principles of the present invention fall within the scope of protection requested by the present invention.

[0058] Formulation Examples

[0059] The percentages in the formulas of all preparations used in the present invention are all weight percentages, the active components in the formulas are metered and added after 100% reduction, and the auxiliary agents and raw materials are conventional commercially available varieties.

[0060] Example 1: 10% FM-86·Copper hydroxide suspension (1:1)

[0061] FM-86 5%, copper hydroxide 5%, dispersant Atlox 4913 2%, wetting agent TXC 2%, agricultural milk 1601# 2%, white carbon black 2%, xanthan gum 0.2%, SAG1522 1%, ethylene glycol 5%, water to 100%.

[0062] Example 2: 30% FM-86·Copper hydroxide suspension (1:2)

[0063] FM-86 10%, copper hydroxide 20%, dispersant Atlox 4913 5%, emulsifier T-20 2%, agricultural milk 700# 3%, xanthan gum 0.2%, propylene glycol 5%, Kasson 0.5%, silicone defoamer 0.2%, water to 100%.

[0064] Example 3: 20% FM-86·Copper hydroxide suspension (1:3)

[0065] FM-86 5%, copper hydroxide 15%, polycarboxylate dispersant GY-D800 2%, xanthan gum 0.2%, magnesium aluminum silicate 1%, ethylene glycol 5%, sodium benzoate 0.3%, silicone defoamer 0.5%, water to 100%.

[0066] Example 4: 20% FM-86·Copper hydroxide dispersible oil suspension (1:1)

[0067] FM-86 10%, copper hydroxide 10%, alkylnaphthalene sulfonate formaldehyde condensate NNO 3%, NongRu 500# 5%, BY-125 5%, NongRu 1601# 4%, bentonite 1%, light mineral oil to 100%.

[0068] Example 5: 30% FM-86·Copper hydroxide dispersible oil suspension (1:2)

[0069] FM-86 10%, copper hydroxide 20%, dispersant SP-0F3468 6%, dispersant SP-0F3472 6%, agricultural milk 1601# 2%, bentonite 2%, methyl oleate to 100%.

[0070] Example 6: 36% FM-86·Copper hydroxide dispersible oil suspension (1:3)

[0071] FM-86 9%, copper hydroxide 27%, alkylnaphthalene sulfonate formaldehyde condensate NNO 3%, NongRu 500# 5%, BY-125 5%, NongRu 1601# 4%, bentonite 1%, light mineral oil to 100%.

[0072] Example 7: 20% FM-86·Copper hydroxide water dispersible granules (1:1)

[0073] FM-86 10%, copper hydroxide 10%, Morwet EFW 2%, dispersant D425 4%, soluble starch 10%, ammonium sulfate 8%, diatomaceous earth to 100%.

[0074] Example 8: 30% FM-86·Copper hydroxide water dispersible granules (1:2)

[0075] FM-86 10%, copper hydroxide 20%, wetting agent GEROPONL-WET / P 2%, dispersant SK-20TX 4%, white carbon black 1%, ammonium sulfate 8%, diatomaceous earth to 100%.

[0076] Example 9: 36% FM-86·Copper hydroxide water dispersible granules (1:3)

[0077] FM-86 9%, copper hydroxide 27%, Morwet EFW 2%, dispersant D425 4%, white carbon black 1%, ammonium sulfate 8%, calcined kaolin to 100%.

[0078] Example 10: 10% FM-86·Copper hydroxide wettable powder (1:1)

[0079] FM-86 5%, copper hydroxide 5%, Morwet EFW 2%, dispersant D425 4%, white carbon black 5%, light calcium carbonate to 100%.

[0080] Example 11: 30% FM-86·Copper hydroxide wettable powder (1:2)

[0081] FM-86 10%, copper hydroxide 20%, wetting agent GEROPONL-WET / P 2%, dispersant SK-20TX 4%, white carbon black 5%, calcined kaolin to make up to 100%.

[0082] Example 12: 20% FM-86·Copper hydroxide wettable powder (1:3)

[0083] FM-86 5%, copper hydroxide 15%, Morwet EFW 2%, dispersant D425 4%, pull open powder BX 2%, white carbon black 5%, light calcium carbonate is supplemented to 100%.

[0084] Example 13: 10% FM-86·Copper hydroxide emulsifiable concentrate (1:1)

[0085] FM-86 5%, copper hydroxide 5%, emulsifier T-20 3%, agricultural milk 2201 10%, ethyl acetate 20%, biodiesel to 100%.

[0086] Example 14: 15% FM-86·Copper hydroxide emulsion (2:1)

[0087] FM-86 10%, copper hydroxide 5%, Nong Ru 500# 4%, Nong Ru 700# 3%, Nong Ru 1601# 5%, ethyl acetate 30%, solvent oil to 100%.

[0088] Example 15: 15% FM-86·Copper hydroxide microemulsion (1:2)

[0089] FM-86 5%, copper hydroxide 10%, Nong Ru 600# 8%, Nong Ru 500# 6%, cyclohexanone 20%, N-methylpyrrolidone 10%, epichlorohydrin 1%, water to 100%.

[0090] Example 16: 20% FM-86·Copper hydroxide aqueous emulsion (1:3)

[0091] FM-86 5%, copper hydroxide 15%, agricultural milk T-20 8%, agricultural milk S-85 4%, tributyl phosphate 5%, solvent oil 2005%, water to 100%.

[0092] Example 17: 15% FM-86·Copper hydroxide emulsifiable powder (1:2)

[0093] FM-86 5%, copper hydroxide 10%, NongRu 1601# 2%, NongRu 500# 3%, NongRu 0201B 5%, N-methyl-pyrrolidone 5%, white carbon black 5%, bentonite to make up to 100%.

[0094] Example 18: 20% FM-86·Copper hydroxide granules (1:3)

[0095] FM-86 5%, copper hydroxide 15%, tributyl phosphate 3%, epichlorohydrin 2%, sodium sulfate 10%, diatomaceous earth to 100%.

[0096] Example 19: 20% FM-86·Copper hydroxide emulsifiable granules (1:3)

[0097] FM-86 5%, copper hydroxide 15%, Nong Ru 0201B 4%, Nong Ru 700# 2%, soluble starch 15%, N-methyl-pyrrolidone 3%, white carbon black 5%, light calcium carbonate to 100%.

[0098] Example 20: 50% FM-86·Copper hydroxide dry suspension (1:1)

[0099] FM-86 25%, copper hydroxide 25%, sodium lignin sulfonate 4%, wetting agent TXC 2%, white carbon black 2%, kaolin to 100%.

[0100] Example 21: 28% FM-86·Copper hydroxide tablets (1:3)

[0101] FM-86 7%, copper hydroxide 21%, Morwet EFW 2%, sodium dodecyl sulfate 1%, soluble starch 15%, sodium sulfate 8%, white carbon black 5%, diatomaceous earth to 100%.

[0102] Example 22: 18% FM-86·Copper hydroxide tablets (5:1)

[0103] FM-86 15%, copper hydroxide 3%, Morwet EFW 2%, dispersant D425 4%, ammonium sulfate 4%, soluble starch 20%, calcined kaolin to 100%.

[0104] Example 23: 15% FM-86·Copper hydroxide effervescent tablets (1:2)

[0105] FM-86 5%, copper hydroxide 10%, alkylnaphthalene sulfonate formaldehyde condensate NNO 4%, sodium lauryl sulfate 2%, citric acid 5%, soluble starch 20%, calcined kaolin to 100%.

[0106] Example 24: 16% FM-86·Copper hydroxide effervescent tablets (3:1)

[0107] FM-86 12%, copper hydroxide 4%, sodium dibutylnaphthalene sulfonate (pulled powder BX) 4%, calcium lignin sulfonate 2%, sodium carbonate 5%, soluble starch 20%, calcined kaolin to make up to 100%.

[0108] Bioassay Example

[0109] Example 25: Biological Activity Test

[0110] The method of protection test was adopted. Specifically, during the period of new citrus shoots, which is susceptible to citrus canker, the citrus leaves were first treated with post-wound spraying of the agent. After the agent was naturally dried in the shade, the citrus canker bacteria were artificially inoculated. According to Sun Yunpei's co-toxicity coefficient method, the co-toxicity coefficient was between 80-120, indicating that there was no obvious interaction between the single agents after mixing, showing an additive effect of drug efficacy. When the co-toxicity coefficient was less than 80, it indicated that there was a significant reduction in efficacy between the single agents after mixing. When the co-toxicity coefficient was greater than 120, it indicated that the two agents showed a synergistic effect. The results of the combined toxicity test of FM-86 and copper hydroxide are shown in Table 1. In the joint toxicity test, when the ratio of FM-86 to copper hydroxide was 5:1, 3:1, and 1:5, the co-toxicity coefficient of the mixed control of citrus canker was between 80 and 120, showing an additive effect of drug efficacy. When the ratio of FM-86 to copper hydroxide is between 1:1 and 1:3, the co-toxicity coefficient of the mixture is greater than 120, showing a synergistic effect.

[0111] Table 1: Calculation results of the co-toxicity coefficient of FM-86 and copper hydroxide mixed to control citrus canker

[0112]

[0113] The results show that the combination of FM-86 and copper hydroxide has a synergistic effect. Within the measured range of 1:1 to 3, the combination has a good effect, significant synergy, and can effectively reduce the dosage.

[0114] Example 26: Field efficacy test of the suspension concentrate of Example 2 (30% FM86 + copper hydroxide SC) for controlling citrus canker

[0115] Test crops: Citrus varieties: Wogan

[0116] Target: Citrus canker (Xanthomonas axonopodis pv.citri)

[0117] Table 2: Test agents

[0118] Chinese name / dosage form content Product Name Production Unit Experimental Drugs Example 2 Suspension — Guangxi Siyue Biotechnology Co., Ltd. Control drug 37.5% Coronavirus SC Corona Fungus Nufarm Australia Limited Clear water control Blank control — —

[0119] Suitable application period and dosage: Use before or at the early stage of the disease. For young fruit trees, the main purpose is to protect the shoots: When the shoots of citrus are 3-5cm long in spring, summer and autumn, start to use the medicine for prevention and control, and apply the medicine again after an interval of about 10 days, and apply the medicine continuously for 3-4 times. For adult fruit trees, the main purpose is to protect the fruit: Apply the medicine once after the flowers fall, and apply the medicine continuously for 3-4 times.

[0120] Recommended concentration: The recommended economical and effective concentration in production is 400-600 mg / kg.

[0121] How to use: Use the spray method to evenly spray the back and front of the leaves (fruits) until they are moist.

[0122] Survey method: 20 trees were surveyed in each treatment area, and samples were taken from each tree at five points: east, west, south, north, and center. All leaves on two branches were surveyed at each point, and the number of leaves (fruits) at each level was recorded.

[0123] Leaf (fruit) grading method

[0124] Level 0: no spots;

[0125] Level 1: 1 to 5 lesions on each leaf (fruit);

[0126] Level 3: There are 6 to 10 lesions on each leaf (fruit);

[0127] Level 5: There are 11 to 15 lesions on each leaf (fruit);

[0128] Level 7: 16 to 20 lesions on each leaf (fruit);

[0129] Level 9: There are more than 21 lesions on each leaf (fruit);

[0130] Calculation method of drug efficacy:

[0131] Disease index = {∑[number of diseased leaves (fruits) at each level × relative level value] / total number of leaves (fruits) investigated × 9} × 100

[0132] Control effect (%) = [(disease index of blank control area - disease index of treated area) / disease index of blank control area] × 100

[0133] The test results are shown in Table 3.

[0134] Table 3: Pesticide efficacy

[0135]

[0136] The results showed that the experimental agent 30% FM-86·Copper Hydroxide SC had a relatively ideal control effect on citrus canker. Under the premise of reducing the dosage of heavy metal copper ions, the field control effect on citrus canker could reach at least 80%, which could effectively control the occurrence and damage of canker in the field and reduce the yield loss caused by canker infection.

[0137] In summary, the FM-86 and copper hydroxide antibacterial composition of the present invention has a significant effect in preventing and treating citrus canker, and has good safety for target crops. The compound preparation not only improves the control effect, but also expands the control range of bacteria, so the compound composition is of great significance for production practice.

[0138] It should be understood that the above embodiments are exemplary and are not intended to include all possible implementations included in the claims. Various modifications and changes may be made on the basis of the above embodiments without departing from the scope of the present disclosure. Similarly, the various technical features of the above embodiments may be arbitrarily combined to form other embodiments of the present invention that may not be explicitly described. Therefore, the above embodiments only express several implementations of the present invention and do not limit the scope of protection of the patent of the present invention.

Claims

1. A bacterial inhibitor composition, characterized in that, the active ingredients of the bacterial inhibitor composition include: malononitrile compounds and copper hydroxide.

2. The bacterial inhibitor composition according to claim 1, characterized in that, the structure of the malononitrile compound is shown in Formula I:

3. The bacterial inhibitor composition according to claim 2, characterized in that, the weight ratio of the malononitrile compound to copper hydroxide is 1:100 - 100:1; preferably, the weight ratio of the malononitrile compound to copper hydroxide is 1:50 - 50:1; more preferably, the weight ratio of the malononitrile compound to copper hydroxide is 1:10 - 10:1; further preferably, the weight ratio of the malononitrile compound to copper hydroxide is 1:1 - 1:

3.

4. The bacterial inhibitor composition according to any one of claims 1 - 3, characterized in that, the bacterial inhibitor composition can be prepared into any agriculturally acceptable dosage form; preferably, the dosage form includes emulsifiable concentrate, microemulsion, aqueous emulsion, suspension concentrate, oil-dispersible suspension concentrate, wettable powder, emulsifiable powder, granule, water-dispersible granule, emulsifiable granule, dry suspension, tablet, effervescent tablet; more preferably, the dosage form is wettable powder, or water-dispersible granule, or suspension concentrate.

5. The bacterial inhibitor composition according to claim 1, characterized in that, the bacterial inhibitor composition further includes at least one adjuvant and / or at least one carrier; preferably, the active ingredients in the bacterial inhibitor composition account for 0.5% - 95% of the total weight; preferably 1% - 90%.

6. Use of the bacterial inhibitor composition according to any one of claims 1 - 5 in the prevention and / or treatment of plant bacterial diseases.

7. The use according to claim 6, characterized in that, the plant bacterial diseases include cucumber bacterial angular leaf spot, tobacco wildfire caused by Pseudomonas spp., rice bacterial blight, rice bacterial leaf streak, cucumber bacterial leaf blight, cabbage black rot, citrus canker caused by Xanthomonas spp., Chinese cabbage soft rot, pear fire blight caused by Erwinia spp., tomato and peanut bacterial wilt caused by Ralstonia spp., tomato canker caused by Clavibacter spp.; preferably, the plant bacterial disease is citrus canker.

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