Feed colorant composition and application thereof in improving skin color of aquatic animals

Abstract

The invention relates to the technical field of feeding colorants, in particular to a feeding colorant composition and application thereof in improving the skin color of aquatic animals. The feed colorant composition provided by the invention has the efficacy of improving the skin coloring effect of the aquatic animals, and when the feed colorant composition is used for feeding the aquatic animals, the yellow chromaticity of skin tissues of the aquatic animals can be remarkably improved, and particularly, the feed colorant composition has an excellent yellow coloring effect on back and abdomen skins. The feeding colorant composition can be used as a feed additive, can be added into a daily feed for aquatic animals such as cultured fishes for feeding, realizes simultaneous improvement of skin color and brightness, has an effect obviously better than that of an existing colorant, and has a relatively good application prospect in aquatic animal culture.

Description

Technical Field

[0001] The present invention relates to the technical field of feed colorants, and particularly to a feed colorant composition and its application in improving the skin color of aquatic animals. Background Art

[0002] With the continuous improvement of people's living standards, the quality of artificially cultured aquatic products has received increasing attention. The market value and ornamental value of many aquatic products are largely affected by their external morphology and body color. The rich and colorful body colors of aquatic animals are mainly attributed to the role of carotenoids. However, since they lack the ability to synthesize such pigments themselves, they must obtain them from food sources. However, with the intensive development of the aquaculture industry, the breeding scale of economic fish has expanded rapidly, and the breeding cycle has gradually shortened. After feeding a large amount of artificial compound feed, the body colors of these fish often become lighter or even show abnormal phenomena, resulting in a significant reduction in their commercial value.

[0003] The currently used yellow colorants have not yet achieved a stable yellow coloring effect in improving the body colors of aquatic animals such as Pelteobagrus fulvidraco and soft-shelled turtles that have a yellow coloring requirement. Therefore, developing an economical, healthy and environmentally friendly yellow coloring composition is of great significance for enhancing the yellow body color of aquatic animals such as Pelteobagrus fulvidraco and soft-shelled turtles that have a yellow coloring requirement. Summary of the Invention

[0004] The present invention provides a feed colorant composition and its application in improving the skin color of aquatic animals.

[0005] Aiming at the deficiencies of the existing technology, the present invention provides a colorant composition for the yellow coloring requirement of the body color (i.e., skin color) of aquatic animals. Feeding aquatic animals with the colorant composition of the present invention can significantly improve the yellow color and brightness of their skin and enhance the coloring effect of the skin.

[0006] Specifically, the present invention provides the following technical solutions.

[0007] In a first aspect, the present invention provides a feed colorant composition, which comprises zeaxanthin and anhydrous lutein in a mass ratio of 1:(5 - 20).

[0008] In the present invention, the anhydrous lutein is preferably anhydrous lutein I, with the chemical formula C 40 H 54 O. The chemical formula of zeaxanthin is C 43 H 62 O 3 , and it can be prepared by methods such as plant extraction and chemical synthesis. Both zeaxanthin and anhydrous lutein can be obtained through commercial channels.

[0009] The present invention has found that although feeding aquatic animals with zeaxanthin and anhydrolutein separately can also endow their body colors with a certain degree of yellow and brightness, the yellow and brightness are both relatively poor, especially the coloring effect on the abdominal skin is even worse. However, when zeaxanthin and anhydrolutein are compounded and used according to the above mass ratio, the yellow and brightness of the dorsal and abdominal skins can be significantly improved, and the coloring effect of feeding with the same dose is significantly better than that of using them separately.

[0010] In some embodiments of the present invention, the feed coloring agent composition comprises zeaxanthin and anhydrolutein in a mass ratio of 1:(5 - 10).

[0011] In some embodiments of the present invention, the feed coloring agent composition consists of zeaxanthin and anhydrolutein in a mass ratio of 1:(5 - 10). When it consists only of zeaxanthin and anhydrolutein, the ratio of the two is preferably controlled within the range of 1:(5 - 10).

[0012] Furthermore, the above-mentioned feed coloring agent composition further comprises lutein, and the lutein is provided in the form of lutein monomer, lutein ester or other stable forms of lutein other than lutein ester; wherein, the mass ratio of zeaxanthin, anhydrolutein and lutein is 1:(5 - 20):(5 - 20).

[0013] Lutein, also known as phytoxanthin, is a kind of carotenoid with the chemical formula C 40 H 56 O 2 , and is widely present in plants such as vegetables, fruits, and flowers. Lutein has been widely used as a yellow coloring agent in seasonings, tobacco, pastries, candies and various feed processing. In the present invention, there is no special requirement for the mass proportion of trans-lutein in the lutein.

[0014] The present invention has found that when using lutein alone as a coloring agent to feed aquatic animals, its coloring effect on the animal skin is limited, especially the coloring effect on the abdominal skin is relatively poor. However, when lutein is added to the above-mentioned coloring agent composition and compounded with zeaxanthin and anhydrolutein according to the above ratio, the coloring effect of the above-mentioned coloring agent composition can be further improved, and the yellow and brightness of the dorsal and abdominal skins of aquatic animals can be significantly improved. In addition, the coloring effect of compounding lutein with zeaxanthin and anhydrolutein is also significantly better than the coloring effect of compounding lutein with zeaxanthin or lutein with anhydrolutein alone. The above results show that lutein, zeaxanthin and anhydrolutein have a positive cooperative effect in improving the yellow coloring effect and color brightness, and have a synergistic effect in the coloring effect.

[0015] Since lutein is relatively sensitive to light, heat, etc. and has poor stability. Therefore, lutein in the above composition can be added in monomer form, or in the form of lutein ester or other stable forms. When added in the form of lutein ester or other stable forms, its mass can be converted according to the mass of lutein (i.e., monomer) required in the composition.

[0016] In some embodiments of the present invention, the feed coloring agent composition consists of zeaxanthin, anhydrous lutein and lutein with a mass ratio of 1:(5 - 20):(5 - 20).

[0017] Based on the above coloring agent composition with the compounding of lutein, zeaxanthin and anhydrous lutein, the present invention finds that controlling the pH of the composition can further improve its coloring effect.

[0018] Preferably, the pH of the 2% aqueous solution of the feed coloring agent composition is 7.2 - 9.0.

[0019] More preferably, the pH of the 2% aqueous solution of the feed coloring agent composition is 7.8 - 9.0. Even more preferably, the pH of the 2% aqueous solution of the feed coloring agent composition is 8.3 - 9.0.

[0020] The above 2% aqueous solution means that the concentration of the feed coloring agent composition in the aqueous solution is 2%.

[0021] Preferably, the feed coloring agent composition further contains an alkaline pH regulator so that the pH of the 2% aqueous solution of the feed coloring agent composition is 7.8 - 9.0.

[0022] In a second aspect, the present invention provides a feed composition, and the feed composition contains the above-mentioned feed coloring agent composition.

[0023] Optionally, the feed composition further contains one or more selected from oils and fats, vitamins, proteins, carbohydrates, carriers and auxiliaries. Compounding nutrients such as oils and fats, vitamins, proteins, carbohydrates, etc. on the basis of the above coloring agent composition can provide nutrition for animals. Adding excipients such as carriers and auxiliaries can prepare the feed composition into the required dosage forms (such as powders, granules, tablets, suspensions, etc.), and the required carriers or auxiliaries can be added according to the selected dosage form.

[0024] In a third aspect, the present invention provides a feed additive, and the feed additive contains the above-mentioned feed coloring agent composition.

[0025] For the preparation type of the above feed additive, the present invention has no special limitation, and it can be powder, liquid or microcapsule.

[0026] Fourth aspect, the present invention provides a feed, which comprises the above-mentioned feed coloring agent composition or the above-mentioned feed composition or the feed additive.

[0027] Preferably, the mass percentage content of the feed coloring agent composition in the feed is 0.1% - 0.25%.

[0028] Fifth aspect, the present invention provides any one of the following applications of the above-mentioned feed coloring agent composition or the above-mentioned feed composition or the above-mentioned feed additive or the above-mentioned feed: (1) Coloring of animal tissues; (2) Improving the color of animal tissues; (3) Improving the yellow coloring effect of animal tissues.

[0029] Preferably, the animal is an aquatic animal. More preferably, it is a fish, a soft-shelled turtle or a shrimp. Among them, the fish can be any fish with a yellow coloring requirement, including but not limited to common fish with yellow to red body colors such as Pelteobagrus fulvidraco, Larimichthys crocea, Amphilophus citrinellus, and Trionyx sinensis.

[0030] In some embodiments of the present invention, the fish is Pelteobagrus fulvidraco or Trionyx sinensis.

[0031] Preferably, the tissue is the skin.

[0032] Preferably, the application includes: feeding the animal with the feed coloring agent composition or the feed composition or the feed additive or the feed.

[0033] In some embodiments of the present invention, the feed coloring agent composition or the feed composition is added to the daily feed of the aquatic animal for feeding.

[0034] Preferably, the mass ratio of the feed coloring agent composition in the feed is 0.1% - 0.25%.

[0035] In the above (2), improving the color of animal tissues preferably refers to improving the skin color of aquatic animals, including the yellow chroma and / or brightness of the skin.

[0036] In the above (3), the yellow coloring effect includes yellow chroma (b * ), and / or brightness (L * ).

[0037] Sixth aspect, the present invention provides a method for improving the coloring effect of the skin of aquatic animals, the method comprising: feeding the aquatic animal with the feed coloring agent composition or the feed composition or the feed additive or the feed.

[0038] The beneficial effects of the present invention at least include: The feed coloring agent composition provided by the present invention has the effect of improving the skin coloring effect of aquatic animals. Feeding aquatic animals with this feed coloring agent composition can significantly enhance the yellow chromaticity of their skin tissues, especially having an excellent yellow coloring effect on the back and abdominal skin. This feed coloring agent can be used as a feed additive and added to the daily feed of aquatic animals such as farmed fish for feeding, achieving the simultaneous improvement of the skin color and brightness, and the effect is significantly better than existing coloring agents, having good application prospects in the aquaculture of aquatic animals.

[0039] It should be noted that the endpoints and any values within the ranges disclosed herein are not limited to the exact ranges or values. These ranges or values should be understood to include values close to these ranges or values. For numerical ranges, the endpoints of each range, between the endpoints of each range and individual point values, and between individual point values can be combined with each other to obtain one or more new numerical ranges, and these numerical ranges should be regarded as specifically disclosed herein. Detailed implementation manners

[0040] To make the objectives, technical solutions, and advantages of the present invention clearer, the technical solutions in the present invention will be clearly and completely described below. Apparently, the described embodiments are some, but not all, of the embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0041] For those not indicating specific techniques or conditions in the following embodiments, they shall be in accordance with the techniques or conditions described in the literature in this field or in accordance with the product specifications. For reagents or instruments not indicating the manufacturer, they are all conventional products that can be obtained through regular channels.

[0042] In the following embodiments, zeaxanthin, anhydrous lutein, and lutein are all commercially available; among them, anhydrous lutein is anhydrous lutein I.

[0043] Example 1 This example provides a feed coloring agent composition, which is composed of zeaxanthin and anhydrous lutein in a mass ratio of 1:5. The pH of the 2% aqueous solution product of this feed coloring agent composition is 7.5.

[0044] Example 2 This example provides a feed coloring agent composition, which is composed of zeaxanthin and anhydrous lutein in a mass ratio of 1:10. The pH of the 2% aqueous solution product of this feed coloring agent composition is 7.4.

[0045] Example 3 This embodiment provides a feed coloring agent composition, which is composed of zeaxanthin, anhydrous lutein and lutein in a mass ratio of 1:10:20. The pH of the aqueous solution product with a 2% content of this feed coloring agent composition is 7.6.

[0046] Example 4 This embodiment provides a feed coloring agent composition, which is composed of zeaxanthin, anhydrous lutein and lutein in a mass ratio of 1:20:20. The pH of the aqueous solution product with a 2% content of this feed coloring agent composition is 7.3.

[0047] Example 5 This embodiment provides a feed coloring agent composition, which is composed of zeaxanthin, anhydrous lutein and lutein in a mass ratio of 1:15:15. The pH of the aqueous solution product with a 2% content of this feed coloring agent composition is 7.6.

[0048] Example 6 This embodiment provides a feed coloring agent composition, which is composed of zeaxanthin, anhydrous lutein and lutein in a mass ratio of 1:10:10. The pH of the aqueous solution product with a 2% content of this feed coloring agent composition is 7.4.

[0049] Example 7 This embodiment provides a feed coloring agent composition, which is composed of zeaxanthin, anhydrous lutein and lutein in a mass ratio of 1:5:5. The pH of the aqueous solution product with a 2% content of this feed coloring agent composition is 7.5.

[0050] Example 8 This embodiment provides a feed coloring agent composition, which is composed of zeaxanthin, anhydrous lutein and lutein in a mass ratio of 1:5:10. The pH of the aqueous solution product with a 2% content of this feed coloring agent composition is 7.4.

[0051] Example 9 This embodiment provides a feed coloring agent composition, which is composed of zeaxanthin, anhydrous lutein and lutein in a mass ratio of 1:5:10. Meanwhile, the pH of the aqueous solution product with a 2% content of this feed coloring agent composition is adjusted to 7.8 by the processing aid sodium hydroxide.

[0052] Example 10 This embodiment provides a feed coloring agent composition, which is composed of zeaxanthin, anhydrous lutein and lutein in a mass ratio of 1:5:10. Meanwhile, the pH of the aqueous solution product with a 2% content of this feed coloring agent composition is adjusted to 8.0 by the processing aid sodium hydroxide.

[0053] Example 11 This embodiment provides a feed coloring agent composition, which is composed of zeaxanthin, anhydrous lutein and lutein in a mass ratio of 1:5:10. At the same time, the pH value of the 2% content aqueous solution product of this feed coloring agent composition is adjusted to 9.0 by the processing aid sodium hydroxide.

[0054] Example 12 This embodiment provides a feed coloring agent composition, which is composed of zeaxanthin, anhydrous lutein and lutein in a mass ratio of 1:5:10. At the same time, the pH of the 2% content aqueous solution product of this feed coloring agent composition is adjusted to 8.3 by the processing aid sodium hydroxide.

[0055] Comparative Example 1 This comparative example provides a yellow coloring agent, which is lutein. The pH of the 2% content aqueous solution product of this yellow coloring agent is 7.3.

[0056] Comparative Example 2 This comparative example provides a yellow coloring agent, which is zeaxanthin. The pH of the 2% content aqueous solution product of this yellow coloring agent is 7.4.

[0057] Comparative Example 3 This comparative example provides a yellow coloring agent, which is anhydrous lutein. The pH of the 2% content aqueous solution product of this yellow coloring agent is 7.2.

[0058] Comparative Example 4 This comparative example provides a feed coloring agent composition, which is composed of zeaxanthin and lutein in a mass ratio of 1:4. The pH of the 2% content aqueous solution product of this feed coloring agent composition is 7.6.

[0059] Comparative Example 5 This comparative example provides a feed coloring agent composition, which is composed of zeaxanthin and lutein in a mass ratio of 1:8. The pH of the 2% content aqueous solution product of this feed coloring agent composition is 7.4.

[0060] Comparative Example 6 This comparative example provides a feed coloring agent composition, which is composed of zeaxanthin and lutein in a mass ratio of 1:25. The pH of the 2% content aqueous solution product of this feed coloring agent composition is 7.5.

[0061] Comparative Example 7 This comparative example provides a feed coloring agent composition, which is composed of zeaxanthin and anhydrous lutein in a mass ratio of 1:3. The pH of the 2% content aqueous solution product of this feed coloring agent composition is 7.6.

[0062] Comparative Example 8 This comparative example provides a feed coloring composition composed of zeaxanthin and lutein in a mass ratio of 10:1. The pH of the aqueous solution product with a 2% content of this feed coloring composition is 7.4.

[0063] Comparative Example 9 This comparative example provides a feed coloring composition. Based on the optimal ratio of zeaxanthin, anhydrous lutein, and lutein in a mass ratio of 1:5:10, zeaxanthin is removed, that is, it is composed of anhydrous lutein and lutein in a mass ratio of 5:10. The pH of the aqueous solution product with a 2% content of this feed coloring composition is 7.5.

[0064] Experimental Example 1 Feed the yellow catfish with the colorants of the above-mentioned examples and comparative examples, and evaluate the skin coloring effect of each colorant on the yellow catfish.

[0065] Randomly select 90 yellow catfish with the same size, specification, physical health, and no diseases in each example, comparative example, and blank group. Before the formal test, put the yellow catfish into a temporary holding bucket for acclimation for 2 weeks. After the acclimation, select the yellow catfish with uniform specifications and good health, with an average weight of (100 ± 5.21) g, and randomly distribute them into 3 small breeding buckets, with 30 tails in each small bucket. Every 3 small buckets are regarded as 1 group, and feed the experimental group feed (that is, the feed added with the colorants of the above-mentioned examples and comparative examples (the formula is shown in Table 2), and the addition amount of each colorant is 0.15 wt%). At the same time, set up a blank group (the difference from the experimental group is that the feed fed does not add the colorants of the above-mentioned examples and comparative examples). The breeding cycle is 8 weeks, and feed is given regularly 2 times a day. During the test, the control parameters of the breeding water are: temperature 22 - 28 °C, pH 7.0 - 8.5, salinity ≤ 0.7%, alkalinity ≤ 25 mmol / L, and dissolved oxygen content above 3 mg / L.

[0066] After the breeding test, select 5 tails from each replicate. Take the section from the posterior margin of the gill cover to the anus of the yellow catfish as the body color test site (measure 5 sites on the back and abdomen at equal intervals). Before the test, wipe the surface moisture dry with filter paper and zero the color difference meter. For the test of the skin body color parameters, use a color difference meter to detect the brightness, redness, and yellowness values of the fish body in the dark and record (take the average value). The yellow coloring situation of the fish body is shown in Table 1.

[0067] Table 1

[0068] As can be seen from Table 1, for the yellow catfish in Examples 1 - 12, the L * 、b *The increase in value indicates that both the color and brightness of the yellow catfish are significantly improved. Among Examples 1-8, the coloring agent compositions of Examples 3-8 have a better yellow coloring effect on the body color of yellow catfish than Examples 1-2; among Examples 9-12, increasing the pH of the composition can effectively improve the brightness of coloring and the b value of the abdomen. * The coloring agents of Examples 11-12 are superior to Examples 9-10, and the L and b values of the back and abdomen of the yellow catfish are the highest, indicating the best yellow coloring effect; while in Examples 9-10, the L and b values of the back and abdomen of the yellow catfish are relatively high, but the effect is weaker than that of Examples 11-12. * and b * values are the highest, indicating the best yellow coloring effect; while in Examples 9-10, the L and b values of the back and abdomen of the yellow catfish are relatively high, but the effect is weaker than that of Examples 11-12. * and b * values are higher, but the effect is weaker than that of Examples 11-12.

[0069] In Comparative Examples 1-9, using the yellow coloring agent has a yellow coloring effect on the yellow catfish, but the L and b values of the yellow coloring agent on the back and abdomen of the yellow catfish under the same addition amount are lower than those of Examples 1-12. The above results show that the coloring agent composition in the examples of the present invention has a significantly superior yellow coloring effect on the body color of the yellow catfish compared with other yellow coloring agents. * and b * values are lower than those of Examples 1-12. The above results show that the coloring agent composition in the examples of the present invention has a significantly superior yellow coloring effect on the body color of the yellow catfish compared with other yellow coloring agents.

[0070] Experimental Example 2 Different coloring agents were used to feed soft-shelled turtles (i.e., Chinese soft-shelled turtles) to evaluate the skin coloring effect of each coloring agent on soft-shelled turtles.

[0071] The experimental soft-shelled turtles were randomly divided into 8 groups, with 30 tails in each group. The daily feeding amount was 1.5%-3.0% of the body weight and was adjusted accordingly every week according to the feeding situation of the soft-shelled turtles. Feed was given twice a day (8:00, 17:00), and the breeding period lasted for one and a half months. During the breeding period, the temperature, dissolved oxygen, pH, ammonia nitrogen, and nitrite of the breeding water body were continuously monitored. The feeding amount, residual bait amount, weather conditions, water quality conditions, and death number were recorded every day, and ten soft-shelled turtles were taken from each group for color difference analyzer analysis.

[0072] The tests were divided into a blank group, Experimental Group 1, Experimental Group 2, Experimental Group 3, Experimental Group 4, Experimental Group 5, Experimental Group 6, and Experimental Group 7. Among them, the blank group was fed a basal diet (Table 2), and the diets of the other experimental groups were added with the corresponding coloring agent composition on the basis of the basal diet.

[0073] Table 2

[0074] Experimental Group 1: A coloring agent composition composed of zeaxanthin and lutein in a mass ratio of 1:1 was added to the basal diet.

[0075] Experimental Group 2: A coloring agent composition composed of zeaxanthin and lutein added to the basal diet in a mass ratio of 1:10.

[0076] Test group 3: Lutein was added to the basal diet.

[0077] Test group 4: Zeaxanthin was added to the basal diet.

[0078] Test group 5: A coloring agent composition composed of zeaxanthin, anhydrous lutein and lutein added to the basal diet in a mass ratio of 1:5:10; the pH of the aqueous solution product with a 2% content of this feed coloring agent composition is 7.4. (The coloring agent composition of Example 8).

[0079] Test group 6: A coloring agent composition composed of zeaxanthin, anhydrous lutein and lutein added to the basal diet in a mass ratio of 1:5:10, and the pH of the coloring agent composition was adjusted to 8.5 by the processing aid sodium hydroxide.

[0080] Test group 7: A coloring agent composition composed of zeaxanthin, anhydrous lutein and lutein added to the basal diet in a mass ratio of 1:20:20, and the pH of the coloring agent composition was adjusted to 8.5 by the processing aid sodium hydroxide.

[0081] Table 3

[0082] As can be seen from Table 3, the L * value and b * value of each test group are higher than those of the blank group, indicating that each test group has a yellow coloring effect on soft-shelled turtles. The coloring effects of test group 5, test group 6 and test group 7 are significantly better than those of test group 1-4, indicating that the combination of the three components of zeaxanthin, anhydrous lutein and lutein is superior to the single use and the combination of two components. The coloring effects of test group 6-7 are further superior to those of test group 5, indicating that after adjusting the pH of the coloring agent composition, its yellow coloring effect is further improved compared with that without adjusting the pH.

[0083] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A feed colorant composition, characterized in that: The feed colorant composition comprises zeaxanthin and dehydrated lutein in a mass ratio of 1:(5-20).

2. The feed colorant composition according to claim 1, characterized in that The feed colorant composition comprises zeaxanthin and dehydrated lutein in a mass ratio of 1:(5-10).

3. The feed colorant composition according to claim 1, characterized in that The feed colorant composition further comprises lutein, which is provided in the form of monomer, ester or other lutein stable forms other than lutein ester; Among them, the mass ratio of zeaxanthin, dehydrated lutein and lutein is 1: (5-20): (5-20).

4. The feed colorant composition according to any one of claims 1 to 3, characterized in that The pH of a 2% aqueous solution of the feed colorant composition is 7.2-9.

0.

5. The feed colorant composition according to any one of claims 1 to 4, characterized in that The pH of a 2% aqueous solution of the feed colorant composition is 7.8-9.0; Preferably, the feed colorant composition further comprises an alkaline pH regulator, so that the pH of a 2% aqueous solution of the feed colorant composition is 7.8-9.

0.

6. A feeding composition, characterized in that: The feed composition comprises the feed colorant composition according to any one of claims 1 to 5.

7. The feed composition according to claim 6, characterized in that The feeding composition further comprises one or more selected from oil, vitamins, proteins, carbohydrates, carriers and adjuvants.

8. A feed additive, characterized in that: The feed additive comprises the feed colorant composition according to any one of claims 1 to 5.

9. A feed, characterized in that: The feed comprises the feed colorant composition according to any one of claims 1 to 5, the feed composition according to claim 6 or 7, or the feed additive according to claim 8.

10. Any of the following uses of the feed colorant composition according to any one of claims 1 to 5, the feed composition according to claim 6 or 7, the feed additive according to claim 8, or the feed according to claim 9: (1) Animal tissue coloring; (2) Improve the color of animal tissues; (3) Improve the yellow coloring effect of animal tissues; Preferably, the animal is an aquatic animal; more preferably, a fish, a turtle or a shrimp; and / or, the tissue is skin; And / or, the application includes: The feed colorant composition according to any one of claims 1 to 5, the feed composition according to claim 6 or 7, the feed additive according to claim 8, or the feed according to claim 9 is administered to the animal by feeding.

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