Feed additive for enhancing disease resistance of piglets, preparation method and application

Abstract

The invention relates to the technical field of feed additives, in particular to a feed additive for enhancing disease resistance of piglets, a preparation method and application. The composition comprises the following raw materials: 4.6%-7% of gypenoside; 6.6%-9.4% of sodium caseinate; 9%-12% of mannan oligosaccharide; 4%-6% of bacillus; 1.6%-2.4% of vitamin C; according to the feed additive capable of enhancing the disease resistance of the piglets, the preparation method and the application, through the synergistic effect of various raw materials, such as the fiveleaf gynostemma herb saponin for stimulating immune organs, the sodium caseinate for supplementing nutrition, the mannan oligosaccharide and the bacillus for regulating intestinal flora, the vitamin C for resisting oxidation and the like, the disease resistance of the piglets is obviously enhanced; the health level is improved; through the preparation process of the feed additive, all the raw materials can be fully mixed, the uniform distribution of all the components in the additive is improved, the stable effect of the feed additive is improved, and the mixing of impurities and harmful substances is effectively reduced, so that the quality and the use effect of the feed additive are improved.

Description

Technical Field

[0001] The present invention relates to the technical field of feed additives, and specifically, to a feed additive for enhancing the disease resistance of piglets, a preparation method and an application thereof. Background Art

[0002] The weaning stage of piglets is a "high-risk period" in their growth process. In terms of nutritional intake, transitioning from breast milk to feed, for the tender digestive system of piglets, due to the low secretion and activity of digestive enzymes, it is difficult to efficiently decompose macromolecular nutrients in the feed, resulting in frequent problems such as indigestion and nutritional absorption disorders. This not only affects the normal growth of piglets but also greatly weakens their immunity.

[0003] At the same time, the immune system of piglets has not yet developed maturely, the functions of immune cells are not yet perfect, the immune response mechanism is slow and inefficient. The intestine, as the first line of defense against pathogen invasion, due to the fragile mucosal barrier function and the unstable colonization of beneficial bacteria, makes piglets extremely vulnerable to the invasion of pathogens such as Escherichia coli and Salmonella, thereby triggering gastrointestinal diseases such as diarrhea and enteritis. These diseases not only increase the mortality rate of piglets but also increase the breeding cost, including treatment costs, feed waste, and losses caused by the extended growth cycle.

[0004] Existing piglet feed additives can improve the growth conditions of piglets to a certain extent, but there are still many deficiencies. For example, the survival rate of some probiotics is significantly reduced during the high-temperature granulation process and cannot effectively colonize and play a role in the piglet intestine. On the other hand, the functions of existing additives are often relatively single, mostly only focusing on one aspect of nutritional supplementation or intestinal regulation, lacking a comprehensive and integrated regulation of the immune system of piglets, and unable to fundamentally improve the disease resistance of piglets. In addition, in practical applications, there are problems with compatibility with different feeds, and precipitation and stratification are likely to occur, resulting in uneven distribution of feed nutrients, affecting piglet feeding and nutrient intake, thereby reducing the quality and use effect of the feed additive.

[0005] In view of this, there is an urgent need for a feed additive for enhancing the disease resistance of piglets, a preparation method and an application thereof. Summary of the Invention

[0006] The purpose of the present invention is to provide a feed additive for enhancing the disease resistance of piglets, a preparation method and an application thereof to solve the problems raised in the above background art.

[0007] To achieve the above purpose, on the one hand, the present invention provides a preparation method of a feed additive for enhancing the disease resistance of piglets, including the following steps:

[0008] S1. Raw material preparation: Prepare gypenosides, sodium caseinate, mannan oligosaccharides, Bacillus, vitamin C and corn starch;

[0009] S2, Bacillus pretreatment: Pour Bacillus into a container with sterile saline, then shake the container to preliminarily and evenly disperse Bacillus and sterile saline. After that, place it inside a constant-temperature shaking incubator for shaking culture to obtain a Bacillus bacterial liquid in a highly active resuscitated state;

[0010] S3, Initial mixing: Put gypenoside, sodium caseinate, and vitamin C into a blender, turn on the blender for stirring, and at the same time, monitor the material temperature through the temperature sensor inside the blender;

[0011] S4, Mixing mannan oligosaccharide and Bacillus: Add mannan oligosaccharide to the blender. At the same time, use a peristaltic pump to inject the resuscitated Bacillus bacterial liquid into the blender and turn on the blender for stirring;

[0012] S5, Mixing corn starch: Pour corn starch into the inside of a vacuum drying oven, turn on the vacuum drying oven for drying to make the moisture content of corn starch 10% - 12%. Then, pour the dried corn starch into the above-mentioned blender, turn on the blender for stirring to obtain a mixed material;

[0013] S6, Granulation and forming: Use an infrared thermometer to detect the temperature of each part of the mold. Then, convey the mixed material to a flat die granulator, turn on the flat die granulator for granulation. After granulation, take 15 - 20 pieces to measure the compressive strength, and the compressive strength is not less than 6N;

[0014] S7, Drying and finishing: Use a fluidized bed dryer to dry the granulated product to make the moisture content of the product less than 10%, and then the finished feed additive can be obtained.

[0015] As a further improvement of this technical solution, in S1, the fineness of gypenoside is 80 - 100 mesh; the fineness of sodium caseinate is 100 - 120 mesh; the fineness of mannan oligosaccharide is 70 - 90 mesh; Bacillus is in the form of freeze-dried Bacillus powder; the fineness of vitamin C is 60 - 80 mesh; the fineness of corn starch is 120 - 140 mesh.

[0016] As a further improvement of this technical solution, in S2, the temperature of the constant-temperature shaking incubator is constant at 30°C - 32°C, and the constant-temperature shaking incubator continuously shakes at a speed of 100 r / min - 120 r / min for 1.5 h - 2 h.

[0017] As a further improvement of this technical solution, in S3, the blender stirs at a speed of 250 - 270 r / min for 5 - 7 min; the temperature sensor monitors the temperature of the material at 30°C - 35°C.

[0018] As a further improvement of the technical solution, in S4, the mannooligosaccharide is added at a rate of 2 g / s - 3 g / s; the injection rate of the Bacillus bacterium solution is 1 ml / s - 2 ml / s; the blender stirs at a speed of 200 r / min - 220 r / min for 8 min - 10 min.

[0019] As a further improvement of the technical solution, in S5, the vacuum drying oven dries at a temperature of 40°C - 50°C for 2 h - 3 h, and the vacuum degree is 0.08 - 0.09 MPa; the blender stirs at a speed of 150 r / min - 170 r / min for 9 min - 11 min.

[0020] As a further improvement of the technical solution, in S6, the infrared thermometer detects that the temperature difference of each part of the mold does not exceed 3°C; the granulation temperature of the flat die granulator is 55°C - 65°C, the pressure of the flat die granulator is 2 MPa - 3 MPa, and the particle diameter of the granulation by the flat die granulator is 1.5 mm - 2.5 mm.

[0021] As a further improvement of the technical solution, in S7, the fluidized bed dryer continuously dries at a temperature of 45 - 55°C for 1.5 h - 2 h.

[0022] On the other hand, the present invention provides a feed additive for enhancing the disease resistance of piglets prepared by the preparation method described in any one of the above, comprising the following raw materials:

[0023] Gypenoside: 4.6% - 7%;

[0024] Sodium caseinate: 6.6% - 9.4%;

[0025] Mannooligosaccharide: 9% - 12%;

[0026] Bacillus: 4% - 6%;

[0027] Vitamin C: 1.6% - 2.4%;

[0028] Corn starch: the balance.

[0029] The feed additive for enhancing the disease resistance of piglets provided by the present invention is used in the piglet stage of pig breeding.

[0030] Compared with the prior art, the beneficial effects of the present invention:

[0031] 1. In the feed additive, preparation method and application for enhancing the disease resistance of piglets, through the synergistic effect of various raw materials, such as gypenosides stimulating immune organs, sodium caseinate for nutritional supplementation, mannan oligosaccharides and Bacillus for regulating intestinal flora, and vitamin C for antioxidant, etc., the disease resistance of piglets is significantly enhanced and their health level is improved; moreover, through the process of preparing the feed additive, not only can the full mixing of various raw materials be achieved, the uniform distribution of each component in the additive be improved, the stable efficacy of the feed additive be increased, but also the risk of impurities and harmful substances being mixed in can be effectively reduced. At the same time, the inactivation of each raw material due to improper operation during the processing is avoided, thereby improving the quality and use effect of the feed additive.

[0032] 2. In the feed additive, preparation method and application for enhancing the disease resistance of piglets, through the combination of gypenosides and sodium caseinate, on the one hand, the active ingredients of gypenosides are effectively protected and are not easily inactivated in a complex environment, and on the other hand, the release mode of gypenosides is changed, its action time is extended, and the efficacy of immune regulation is increased; combined with the entire preparation process, the produced feed additive has stable and reliable quality. Long-term use can enhance the physique of piglets, improve their resistance to various pathogens, thereby reducing the economic losses in the pig-raising industry. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 It is a flow chart of the preparation method of the feed additive of the present invention;

[0034] Figure 2 It is a bar chart of the immune enhancement effect of the present invention;

[0035] Figure 3 It is a bar chart of the improvement of intestinal health of the present invention;

[0036] Figure 4 It is a bar chart of the growth performance improvement rate of the present invention;

[0037] Figure 5 It is a bar chart of the stability of the present invention;

[0038] Figure 6 It is a bar chart of the disease resistance rate of the present invention. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0039] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments in the present invention, all other embodiments obtained by those skilled in the art without creative efforts belong to the scope of protection of the present invention.

[0040] The embodiments of the present invention provide a feed additive for enhancing the disease resistance of piglets, including the following raw materials:

[0041] Gynostemma pentaphyllum saponins: 4.6% - 7%, Gynostemma pentaphyllum saponins can stimulate the development of immune organs in piglets and enhance the activity of immune cells;

[0042] Sodium caseinate: 6.6% - 9.4%, Sodium caseinate plays an emulsifying and stabilizing role and can also supplement protein for piglets;

[0043] Mannan oligosaccharides: 9% - 12%, Mannan oligosaccharides, as prebiotics, optimize the intestinal flora of piglets, inhibit harmful bacteria, reduce the probability of intestinal infection, and enhance disease resistance;

[0044] Bacillus: 4% - 6%, Bacillus can colonize in the intestinal tract of piglets, secrete antibacterial substances, enhance the intestinal barrier function, and act synergistically with prebiotics;

[0045] Vitamin C: 1.6% - 2.4%, Vitamin C has an antioxidant effect, reduces oxidative damage caused by weaning stress, enhances the body's resistance, and helps piglets grow healthily;

[0046] Corn starch: the balance.

[0047] According to Figure 1 As shown, the embodiment of the present invention also provides a preparation method for preparing the above-mentioned feed additive for enhancing the disease resistance of piglets, including the following steps:

[0048] Step 1, Raw material preparation: Prepare Gynostemma pentaphyllum saponins, sodium caseinate, mannan oligosaccharides, Bacillus, vitamin C, and corn starch; among them: Gynostemma pentaphyllum saponins are in a fineness of 80 - 100 mesh; sodium caseinate is in a fineness of 100 - 120 mesh; mannan oligosaccharides are in a fineness of 70 - 90 mesh; Bacillus is in the form of freeze-dried Bacillus powder; vitamin C is in a fineness of 60 - 80 mesh; corn starch is in a fineness of 120 - 140 mesh;

[0049] Step 2, Pretreatment of Bacillus: Pour Bacillus into a container with sterile physiological saline, and the sterile physiological saline needs to be sterilized by high temperature and high pressure. Then, shake the container to make Bacillus and sterile physiological saline preliminarily evenly dispersed. After that, place it inside a constant temperature shaking incubator for shaking culture. The temperature of the constant temperature shaking incubator is kept constant at 30°C - 32°C, and the constant temperature shaking incubator continuously shakes at a speed of 100 r / min - 120 r / min for 1.5 h - 2 h to obtain a Bacillus bacterial liquid in a highly active resuscitated state;

[0050] Step 3. Primary mixing: Put gypenoside, sodium caseinate and vitamin C into a blender. The blender is a double - helix conical blender. Start the blender for stirring. The blender stirs at a speed of 250 - 270 r / min for 5 - 7 min. When stirring, open the observation window and lighting. If the material adheres to the wall, scrape it off with a silicone spatula. At the same time, monitor the material temperature through the temperature sensor inside the blender. The temperature sensor monitors the material temperature at 30℃ - 35℃. When the material temperature exceeds 35℃, stop stirring and ventilate to cool down;

[0051] Step 4. Mix mannan oligosaccharide and Bacillus: Add mannan oligosaccharide into the blender at a speed of 2 g / s - 3 g / s. At the same time, use a peristaltic pump to inject the resuscitated Bacillus bacterial liquid into the blender at a speed of 1 ml / s - 2 ml / s. Then start the blender for stirring. The blender stirs at a speed of 200 r / min - 220 r / min for 8 min - 10 min;

[0052] Step 5. Mix corn starch: Pour corn starch into the inside of a vacuum drying oven. Start the vacuum drying oven for drying to make the moisture content of the corn starch 10% - 12%. The vacuum drying oven dries at a temperature of 40℃ - 50℃ for 2 h - 3 h, with a vacuum degree of 0.08 - 0.09 MPa. Then, pour the dried corn starch into the above - mentioned blender. Start the blender for stirring. The blender stirs at a speed of 150 r / min - 170 r / min for 9 min - 11 min to obtain a mixed material;

[0053] Step 6. Granulation and forming: Use an infrared thermometer to detect the temperature of each part of the mold. The temperature difference of each part of the mold detected by the infrared thermometer does not exceed 3℃. Then, convey the mixed material to a flat - die granulator. Start the flat - die granulator for granulation. The granulation temperature of the flat - die granulator is 55℃ - 65℃, the pressure of the flat - die granulator is 2 MPa - 3 MPa, and the particle diameter of the granulation by the flat - die granulator is 1.5 mm - 2.5 mm. After granulation, take 15 - 20 grains to measure the compressive strength, and the compressive strength is not less than 6 N;

[0054] Step 7. Drying the finished product: Use a fluidized - bed dryer to dry the granulated product. The fluidized - bed dryer continuously dries at a temperature of 45 - 55℃ for 1.5 h - 2 h to make the moisture content of the product less than 10%, and then the finished feed additive can be obtained.

[0055] In the invention, first, gypenosides are used to endow piglets with immune power. Sodium caseinate has both emulsifying and nutritional supplement functions. Mannan oligosaccharide and Bacillus synergistically optimize the intestinal ecosystem. Vitamin C resists oxidative attacks. Corn starch increases the uniformity of materials. The mixing of various materials can not only strengthen the disease resistance foundation of piglets in multiple dimensions, reduce the risk of infection, and enable them to grow healthily. At the same time, through the preparation process, such as the high-activity resuscitation pretreatment of Bacillus, the effective survival rate and biological activity of the bacterial cells are ensured, and the digestion and absorption efficiency of the feed can also be effectively improved, increasing the high activity, thereby effectively improving the quality and stability of the feed additive finished product;

[0056] In addition, the mixing of gypenosides and sodium caseinate can enhance the immune system of piglets and improve the feed quality. Moreover, through the secondary combination of gypenosides and sodium caseinate, not only can a stable complex structure be formed, effectively preventing the oxidation, degradation and other losses of gypenosides during storage and processing, maintaining the integrity of its active ingredients, but also this combination may change the release characteristics of gypenosides, enabling them to be released more slowly and continuously in the gastrointestinal tract of piglets, prolonging their action time, and enhancing the immune regulation effect. The reaction mechanism mainly lies in the non-covalent binding of functional groups such as hydroxyl and carbonyl groups of gypenoside molecules and amino and carboxyl groups of sodium caseinate molecules through hydrogen bonds, electrostatic interactions, etc.

[0057] Through the synergistic effect of various raw materials, such as gypenosides stimulating immune organs, sodium caseinate for nutritional supplement, mannan oligosaccharide and Bacillus regulating intestinal flora, and vitamin C for antioxidant, etc., the disease resistance ability of piglets is significantly enhanced, and their health level is improved. And through the process of preparing the feed additive, not only can the full mixing of various raw materials be achieved, the uniform distribution of each component in the additive be improved, the stable efficacy of the feed additive be increased, but also the risk of impurities and harmful substances mixing in can be effectively reduced. At the same time, the inactivation of each raw material due to improper operation during the processing is avoided, thereby improving the quality and use effect of the feed additive.

[0058] When gypenosides are combined with sodium caseinate, on the one hand, the active ingredients of gypenosides are effectively protected, making them not easily inactivated in a complex environment. On the other hand, the release mode of gypenosides is changed, prolonging their action time and increasing the efficacy of immune regulation. Combined with the entire preparation process, the produced feed additive has stable and reliable quality. Long-term use can enhance the physique of piglets, improve their resistance to various pathogens, and thus reduce the economic losses of the pig-raising industry.

[0059] According to the usage amounts of different raw materials, the feed additive provided by the present invention will be further described through the following specific embodiments.

[0060] Example 1

[0061] Raw material dosage: gypenoside 4.6%, sodium caseinate 9.4%, mannan oligosaccharide 9%, bacillus 5.2%, vitamin C 1.6%, corn starch 70.2%;

[0062] Preparation process: Strictly follow the standard process. Control the particle size during raw material preparation, 80 mesh for gypenoside and 100 mesh for sodium caseinate; Pretreat the bacillus by oscillating at 30°C and 100 r / min for 1.5 h to fully activate it; For the first mixing, stir at 250 r / min for 5 min, and strictly control the addition speed and stirring time during the subsequent mixing steps; Granulation is carried out at 55°C and 2 MPa, with a particle diameter of 1.5 mm and a compressive strength of 7 N. Finally, dry at 45°C for 1.5 h until the moisture content is less than 10%.

[0063] Example 2

[0064] Raw material dosage: gypenoside 5.5%, sodium caseinate 8.5%, mannan oligosaccharide 11%, bacillus 4.5%, vitamin C 2.4%, corn starch 68.1%;

[0065] Preparation process: 90 mesh for gypenoside and 110 mesh for sodium caseinate. Oscillate the bacillus at 31°C and 110 r / min for 1.7 h. For the first mixing, stir at 260 r / min for 6 min, add mannan oligosaccharide at 2.5 g / s and the bacillus solution at 1.5 ml / s, and stir at 210 r / min for 9 min, and stir corn starch at 160 r / min for 10 min. Granulation is carried out at 60°C and 2.5 MPa, with a particle diameter of 2 mm and a compressive strength of 7.5 N, and dry at 50°C for 1.7 h.

[0066] Example 3

[0067] Raw material dosage: gypenoside 6.3%, sodium caseinate 7.5%, mannan oligosaccharide 10%, bacillus 4%, vitamin C 2.2%, corn starch 70%;

[0068] Preparation process: 100 mesh for gypenoside and 120 mesh for sodium caseinate. Oscillate the bacillus at 32°C and 120 r / min for 2 h. For the first mixing, stir at 270 r / min for 7 min, and follow the standard operation for subsequent mixing. Granulation is carried out at 65°C and 3 MPa, with a particle diameter of 2.5 mm and a compressive strength of 8 N, and dry at 55°C for 2 h.

[0069] Example 4

[0070] Raw material dosage: gypenoside 7%, sodium caseinate 6.6%, mannan oligosaccharide 12%, bacillus 6%, vitamin C 1.8%, corn starch 66.6%;

[0071] Preparation process: The same as that of Example 1.

[0072] Table 1 Dosages of Raw Materials in Examples 1 - 4

[0073] Example 1 Example 2 Example 3 Example 4 Gypenoside % 4.6 5.5 6.3 7 Sodium caseinate % 9.4 8.5 7.5 6.6 Mannan oligosaccharide % 9 11 10 12 Bacillus % 5.2 4.5 4 6 Vitamin C % 1.6 2.4 2.2 1.8 Corn starch % 70.2 68.1 70 66.6

[0074] In order to verify that the feed additive prepared in the examples of the present invention has good effectiveness and stability, the feed additive provided in the examples of the present invention is described through the following test examples.

[0075] Test Example

[0076] The purpose of this test group is to explore the effects of different component ratios on the feed additive, and to detect the immune enhancement effect, improvement of intestinal health, growth performance improvement rate, stability and disease resistance rate of the feed additive of the present invention.

[0077] Test Objectives: Test Group A, Test Group B, Test Group C and Test Group D respectively adopt the component ratios of the feed additives provided in Examples 1 - 4; the control examples adopt Control Group A, Control Group B, Control Group C and Control Group D, where:

[0078] Control Group A

[0079] Formula: Mainly contains specific proportions of minerals, vitamins, amino acids and a small amount of plant extracts, but does not use gypenosides and sodium caseinate;

[0080] Preparation Method: First, each raw material is separately pulverized to the specified fineness, and then stirred and mixed in a common stirring device at 180 - 200 r / min for 6 - 8 min. After mixing evenly, it is directly granulated; the granulation temperature is controlled at 60 - 70 °C, the pressure is 1.5 - 2 MPa, and the particle compressive strength is not strictly detected after granulation. Finally, it is simply dried to a moisture content below 12%.

[0081] Control Group B

[0082] Formula: Select a common feed additive formula on the market that features enhancing the disease resistance of piglets. The main components are a variety of vitamins and mineral premixes, with a small amount of chemically synthesized antibacterial agents added.

[0083] Preparation Method: According to the production process in the product manual, the raw materials are simply mixed and stirred. The stirring speed and time are not strictly and precisely controlled, approximately stirring at 150 - 170 r / min for 5 - 7 min; in the granulation process, an ordinary granulator is used, and the temperature and pressure fluctuate greatly, in the ranges of 50 - 65 °C and 1 - 2.5 MPa respectively. After granulation, the product is not carefully quality - tested, only routine moisture detection is carried out, and the moisture content after drying is controlled at 10 - 13%.

[0084] Control Group C

[0085] Dosage of raw materials: 7% of gypenosides, 12% of mannan oligosaccharides, 6% of Bacillus, 2.4% of vitamin C, 72.6% of corn starch, without sodium caseinate;

[0086] Preparation process: same as Example 1, but without the process of sodium caseinate.

[0087] Control group D

[0088] Dosage of raw materials: 9% of sodium caseinate, 12% of mannan oligosaccharides, 6% of Bacillus, 2.4% of vitamin C, 70.6% of corn starch, without gypenosides;

[0089] Preparation process: same as Example 1, but without the process of gypenosides.

[0090] Test method: According to the immune enhancement effect, improvement of intestinal health, growth performance improvement rate, stability and disease resistance rate of the feed additive of the present invention, tests are carried out respectively, and the specific test methods are as follows:

[0091] Immune enhancement effect:

[0092] After piglets are born, healthy individuals with similar weights are selected and randomly divided into experimental groups and control groups. The experimental groups are fed with feeds containing the feed additives of the corresponding examples, and the control groups are fed with the corresponding control feeds. After the feeding period ends, the piglets are slaughtered, the immune organ indices are measured, peripheral blood mononuclear cells are separated by density gradient centrifugation, the activities of immune cells are detected by MTT method or flow cytometry, and then the levels of immune factors such as interleukin and interferon in the serum are detected by ELISA to evaluate the immune enhancement effect of the feed additive by comparison.

[0093] Improvement of intestinal health:

[0094] During the test period, piglet fecal samples are collected regularly, and the types and relative abundances of intestinal flora are analyzed by high-throughput sequencing, and the changes in the numbers of beneficial bacteria and harmful bacteria are concerned. After the test ends, intestinal tissue samples are obtained from the slaughtered piglets, paraffin sections are made, and after HE staining, the intestinal mucosa integrity, intestinal villus length and crypt depth are observed under a microscope to judge the improvement effect of the feed additive on intestinal health.

[0095] Growth performance improvement rate:

[0096] At the beginning and end of the test, each piglet in each group is weighed on an empty stomach, and the daily feed feeding amount and remaining amount are recorded in detail to calculate the average daily feed intake. By calculating key indicators such as average daily weight gain and feed-to-meat ratio, the differences in growth rate and feed conversion efficiency among different groups of piglets are compared to intuitively show the promoting effect of the feed additive on the growth performance of piglets.

[0097] Stability:

[0098] The feed additives of each test group and the control group were respectively placed in accelerated aging environments such as high temperature, high humidity, and light. Appearance observation was carried out at regular intervals to detect whether there were phenomena such as caking, discoloration, and mildew. At the same time, analytical techniques such as high performance liquid chromatography were used to determine the content changes of each active ingredient in the additive to evaluate its stability.

[0099] Disease resistance rate:

[0100] In the later stage of the experiment, common pathogens such as Escherichia coli and Salmonella were artificially inoculated into the pigsty. The morbidity conditions of each group of piglets were observed and recorded, including the onset time, symptoms, morbidity rate, mortality rate, etc. The disease resistance rates of the piglets in each test group and the control group were obtained through statistical analysis to evaluate the improvement effect of the feed additive on the disease resistance ability of piglets.

[0101] The specific detection indexes are shown in Table 2.

[0102] Table 2 Detection indexes of each sample

[0103]

[0104]

[0105] According to Figure 2 - Figure 6 and Table 2, the summary of the above comparison data is as follows:

[0106] Immune enhancement effect: The immune enhancement effect scores of test groups A - D were all above 85 points, significantly higher than those of the control group. Among them, control group A only got 60 points because it did not use gypenosides and sodium caseinate; in the test groups, gypenosides stimulated the development of immune organs, and sodium caseinate supplemented protein. The two worked together with other components to greatly enhance the activity of immune cells and the level of immune factors.

[0107] Improvement of intestinal health: The test groups also showed excellent performance in improving intestinal health, with scores all above 82 points. Due to problems with the ingredients and preparation process, the scores of the control group were relatively low. In the present invention, mannan oligosaccharides and Bacillus spp. worked together to optimize the intestinal flora and maintain the integrity of the intestinal mucosa. For example, the 86 points of test group C reflected a good effect of improving intestinal health, while control groups C and D, which lacked key components, only got 75 points and 76 points, highlighting the importance of the complete formula.

[0108] Growth performance improvement rate: The growth performance improvement rate scores of test groups A - D were between 80 - 87 points, significantly higher than those of the control group. Through reasonable raw material collocation, the present invention improved the digestion and absorption efficiency of the feed and promoted the growth of piglets. For example, control group A only got 60 points, while test group B reached 83 points due to the appropriate raw material ratio, indicating that the feed additive of the present invention had a significant promoting effect on the growth rate and feed conversion efficiency of piglets.

[0109] Stability: In terms of stability, the scores of experimental groups A - D were all above 88 points, showing good stability. In contrast, the stabilities of control groups A and B were relatively poor, being 70 points and 75 points respectively. For example, in the high - performance liquid chromatography detection of experimental group D, the content change of the active ingredient was small, and there were no obvious phenomena such as caking and color change in appearance, proving that the preparation process of the present invention has a significant effect on improving the product stability.

[0110] Disease resistance rate: The disease resistance rate scores of the experimental groups were all above 86 points, far higher than those of the control groups. After artificial inoculation of pathogens, the feed additive of the present invention significantly reduced the incidence and mortality of piglets. For example, the disease resistance rate of control group B was only 70 points, while that of experimental group C reached 89 points. This is due to the synergistic effect of various raw materials strengthening the disease resistance foundation of piglets, reflecting the important value of the feed additive of the present invention in improving the disease resistance ability of piglets in the actual breeding environment.

[0111] Thus, through the synergistic effect of various raw materials such as gypenosides, sodium caseinate, mannan - oligosaccharides, bacillus, and vitamin C, it performs excellently in aspects such as immune enhancement effect, improvement of intestinal health, growth performance improvement rate, stability, and disease resistance rate, and the scores are far higher than those of control group 2 and control group 3. And through the preparation of the feed additive, it can not only effectively improve the immune function and intestinal health of piglets, but also improve the digestion and absorption efficiency and stability of the feed, reduce the incidence and mortality of piglets, thereby improving the quality and use effect of the feed additive.

[0112] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above - mentioned embodiments. The above - mentioned embodiments and the descriptions in the specification are only preferred examples of the present invention and are not used to limit the present invention. Without departing from the spirit and scope of the present invention, the present invention will have various changes and improvements, and these changes and improvements all fall within the scope of the present invention claimed. The scope of the present invention claimed is defined by the appended claims and their equivalents.

Claims

1. A method for preparing a feed additive for enhancing the disease resistance of piglets, characterized in that: The following steps are involved: S1. Raw material preparation: prepare gypenosin, sodium caseinate, mannooligosaccharide, Bacillus, vitamin C and corn starch; S2. Bacillus pretreatment: Pour the Bacillus into a container with sterile saline, then shake the container to make the Bacillus and the sterile saline preliminarily evenly dispersed, and then place it in a constant temperature shaking incubator for shaking culture to obtain a Bacillus liquid in a highly active recovery state; S3, initial mixing: putting gypenoside, sodium caseinate and vitamin C into a blender, turning on the blender for blending, and monitoring the material temperature through a temperature sensor inside the blender; S4, mixing manno-oligosaccharide and Bacillus: adding manno-oligosaccharide into a blender, and at the same time, injecting the revived Bacillus liquid into the blender using a peristaltic pump, and turning on the blender for stirring; S5, mixing corn starch: pouring corn starch into a vacuum drying oven, turning on the vacuum drying oven to dry the corn starch to make the moisture content of the corn starch 10%-12%, then pouring the dried corn starch into the above-mentioned mixer, turning on the mixer to stir, and obtaining a mixed material; S6, granulation and molding: Use an infrared thermometer to detect the temperature of each part of the mold, then convey the mixed material to the flat die granulator, start the flat die granulator for granulation, and after granulation, draw 15-20 pellets to measure the compressive strength, which is not less than 6N; S7. Drying the finished product: Use a fluidized bed dryer to dry the granulated product to reduce the moisture content of the product to less than 10%, and the finished feed additive product can be obtained.

2. The method for preparing a feed additive for enhancing the disease resistance of piglets according to claim 1, characterized in that: In S1, the fineness of gypenosin is 80-100 mesh; the fineness of sodium caseinate is 100-120 mesh; the fineness of manno-oligosaccharide is 70-90 mesh; the fineness of Bacillus is freeze-dried Bacillus powder; the fineness of vitamin C is 60-80 mesh; and the fineness of corn starch is 120-140 mesh.

3. The method for preparing a feed additive for enhancing the disease resistance of piglets according to claim 1, characterized in that: In S2, the temperature of the constant temperature shaking incubator is kept constant at 30°C-32°C, and the constant temperature shaking incubator is continuously shaken at a speed of 100r / min-120r / min for 1.5h-2h.

4. The method for preparing a feed additive for enhancing the disease resistance of piglets according to claim 1, characterized in that: In S3, the mixer stirs at a speed of 250-270 r / min for 5-7 min; the temperature sensor monitors the temperature of the material at 30° C.-35° C.

5. The method for preparing a feed additive for enhancing the disease resistance of piglets according to claim 1, characterized in that: In S4, the speed of adding mannooligosaccharide is 2 g / s-3 g / s; the speed of injecting Bacillus liquid is 1 ml / s-2 ml / s; and the mixer stirs at a speed of 200 r / min-220 r / min for 8 min-10 min.

6. The method for preparing a feed additive for enhancing the disease resistance of piglets according to claim 1, characterized in that: In S5, the vacuum drying oven is dried at a temperature of 40° C.-50° C. for 2 h-3 h, and the vacuum degree is 0.08-0.09 MPa; the mixer is stirred at a speed of 150 r / min-170 r / min for 9 min-11 min.

7. The method for preparing a feed additive for enhancing the disease resistance of piglets according to claim 1, characterized in that: In S6, the temperature difference of each part of the mold detected by the infrared thermometer does not exceed 3°C; the temperature of the flat die granulator is 55°C-65°C, the pressure of the flat die granulator is 2MPa-3MPa, and the diameter of the particles granulated by the flat die granulator is 1.5mm-2.5mm.

8. The method for preparing a feed additive for enhancing the disease resistance of piglets according to claim 1, characterized in that: In S7, the fluidized bed dryer continues drying at a temperature of 45-55° C. for 1.5 h to 2 h.

9. A feed additive prepared by the method for preparing a feed additive for enhancing the disease resistance of piglets according to any one of claims 1 to 8, characterized in that: Including the following ingredients: Gynostemma saponin: 4.6%-7%; Sodium caseinate: 6.6%-9.4%; Manno-oligosaccharides: 9%-12%; Bacillus: 4%-6%; Vitamin C: 1.6%-2.4%; Corn starch: balance.

10. An application of a feed additive for enhancing the disease resistance of piglets, characterized in that: The feed additive for enhancing the disease resistance of piglets as claimed in claim 9 is used in the piglet stage of pig breeding.

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