Composition for improving activity of intestinal digestive enzyme and application thereof

Abstract

The invention provides a composition for improving intestinal digestive enzyme activity and application thereof, and belongs to the technical field of animal medicine. The composition is prepared from the polysaccharide of the root of slender habenaria, ginsenoside Rb1 and ginsenoside Rg1. In the composition, the mass ratio of the polysaccharide to the ginsenoside Rb1 to the ginsenoside Rg1 is 90: 5: 5 or 95: 2.5: 2.5, preferably 90: 5: 5, and acting intestinal digestive enzymes are specifically duodenal trypsin and amylase. Experimental results show that the composition can remarkably and synergistically improve the activity of duodenal trypsin and amylase of laying hens and improve the feed utilization rate, so that the laying rate is increased, the egg weight and the eggshell strength are improved, and the breeding benefits of the laying hens are effectively improved.

Description

Technical Field

[0001] The present invention belongs to the technical field of animal medicine, and particularly relates to a composition for improving the activity of intestinal digestive enzymes and its application. Background Art

[0002] The laying performance of laying hens is one of the important indicators to measure the economic benefits and production level of the poultry industry, directly reflecting the comprehensive level of breeding management, feed utilization and the health status of laying hens. Among the many factors affecting laying performance, the activity of digestive enzymes, as a key physiological index determining the efficiency of nutrient absorption and metabolism, plays a crucial role. The high efficiency of digestive enzymes not only ensures the full decomposition and absorption of nutrients in feed, providing sufficient energy and essential nutrients for laying hens, but also maintains the overall health of laying hens, enhancing their immunity and disease resistance.

[0003] By improving the activity of digestive enzymes in laying hens, the utilization rate of feed can be significantly optimized, reducing feed waste and cost input. This optimization process not only reduces the breeding cost and improves the breeding efficiency, but also promotes the rapid growth and high-efficiency egg production of laying hens by increasing the nutrient absorption efficiency. In addition, the enhancement of digestive enzyme activity directly affects the egg quality, thus meeting the market demand for high-quality eggs and further improving the economic benefits of farmers. Therefore, improving the activity of digestive enzymes in laying hens is the key way to improve the overall breeding efficiency. Summary of the Invention

[0004] The present invention aims to provide a composition, feed and its application for improving the activity of intestinal digestive enzymes, laying performance and egg quality of laying hens. In view of the deficiencies in the prior art, the present invention combines polysaccharide from Diplazium esculentum (Retz.) Sw. with ginsenoside Rb1 and Rg1 in a specific ratio, synergistically improving the activity of intestinal digestive enzymes in laying hens, and further improving the laying performance and egg quality of laying hens.

[0005] Terminology: In the present invention, the term "polysaccharide from Diplazium esculentum (Retz.) Sw." refers to polysaccharide substances extracted from Diplazium esculentum (Retz.) Sw.; the terms "ginsenoside Rb1" and "ginsenoside Rg1" refer to the active ingredients extracted from ginseng. The CAS number of ginsenoside Rb1 is 41753-43-9, and its molecular formula is C 54 H 92 O 23 , the CAS number of ginsenoside Rg1 is 22427-39-0, and its molecular formula is C 42 H 72 O 14 ; the term "intestinal digestive enzyme" refers to the enzymes in the intestine of laying hens that participate in the digestion of proteins, fats and starches, mainly including trypsin, lipase and amylase.

[0006] The technical solution of the present invention includes: In the first aspect, the present invention provides a composition for improving the intestinal digestive enzyme activity of laying hens, which is characterized in that the composition is composed of ophiorrhiza japonica thunb polysaccharide, ginsenoside Rb1 and ginsenoside Rg1.

[0007] Preferably, the ophiorrhiza japonica thunb polysaccharide is prepared by the following preparation method:

[0008] (1) After cleaning and pulverizing the dried ophiorrhiza japonica thunb, it is mixed with water according to a solid-liquid ratio of 1:15;

[0009] (2) After adding cellulase for enzymatic hydrolysis, it is heated to inactivate, and ultrasonic extraction is carried out according to the conditions of ultrasonic power of 250 w, temperature of 60 °C, and time of 45 minutes;

[0010] (3) After centrifuging to collect the supernatant, decolorization treatment is carried out using activated carbon;

[0011] (4) The filtrate is collected by filtration, concentrated, and then the protein is removed using sevage reagent to obtain the crude ophiorrhiza japonica thunb polysaccharide solution;

[0012] (5) After adding 3 times the amount of ethanol for alcohol precipitation, the precipitate is centrifuged and collected and washed to obtain the crude ophiorrhiza japonica thunb polysaccharide;

[0013] (6) After dissolving the crude ophiorrhiza japonica thunb polysaccharide, the ophiorrhiza japonica thunb polysaccharide solution with a molecular weight of 10 kDa - 50 kDa is collected using an ultrafiltration membrane;

[0014] (7) After concentration, it is freeze-dried to obtain the ophiorrhiza japonica thunb polysaccharide.

[0015] Preferably, in the composition, the mass ratio of ophiorrhiza japonica thunb polysaccharide, ginsenoside Rb1 and ginsenoside Rg1 is 90:5:5 or 95:2.5:2.5.

[0016] Preferably, in the composition, the mass ratio of ophiorrhiza japonica thunb polysaccharide, ginsenoside Rb1 and ginsenoside Rg1 is preferably 90:5:5.

[0017] Preferably, the intestinal digestive enzymes are duodenal trypsin and amylase.

[0018] Preferably, the laying hens are preferably Hy-Line Brown laying hens.

[0019] In the second aspect, the present invention provides a feed for improving the intestinal digestive enzyme activity of laying hens. The feed contains the basic diet for laying hens and the above composition, and the addition amount of the composition in the feed is greater than or equal to 0.05%. The feed of the present invention can enhance the digestive ability of laying hens by improving the intestinal digestive enzyme activity, thereby improving the feed conversion rate and overall production efficiency.

[0020] Preferably, the addition amount of the composition in the feed is preferably 0.05%.

[0021] In the third aspect, the present invention provides an application of the above composition in preparing a feed for improving the intestinal digestive enzyme activity of laying hens, and the intestinal digestive enzymes are duodenal trypsin and amylase.

[0022] In the fourth aspect, the present invention provides a composition for improving the laying performance and egg quality of laying hens, which is characterized in that the composition is composed of ophiorrhiza japonica thunb polysaccharide, ginsenoside Rb1 and ginsenoside Rg1. In the composition, the mass ratio of ophiorrhiza japonica thunb polysaccharide, ginsenoside Rb1 and ginsenoside Rg1 is 90:5:5 or 95:2.5:2.5.

[0023] Preferably, in the composition, the mass ratio of ophiorrhiza japonica thunb polysaccharide, ginsenoside Rb1 and ginsenoside Rg1 is preferably 90:5:5.

[0024] Preferably, the laying performance refers to the laying rate, and the egg quality refers to the egg weight and the eggshell strength;

[0025] The ophiorrhiza japonica thunb polysaccharide is prepared according to the above preparation method.

[0026] In the fifth aspect, the present invention provides a feed for improving the laying performance and egg quality of laying hens, which is characterized in that the feed contains a basic diet for laying hens and the above composition, and the addition amount of the composition in the feed is greater than or equal to 0.05%.

[0027] Preferably, the laying performance refers to the laying rate, and the egg quality refers to the egg weight and the eggshell strength.

[0028] In the sixth aspect, the present invention provides an application of a composition in preparing a feed for improving the laying performance and egg quality of laying hens, and the composition is composed of ophiorrhiza japonica thunb polysaccharide, ginsenoside Rb1 and ginsenoside Rg1;

[0029] The ophiorrhiza japonica thunb polysaccharide is prepared according to the above preparation method.

[0030] Preferably, in the composition, the mass ratio of ophiorrhiza japonica thunb polysaccharide, ginsenoside Rb1 and ginsenoside Rg1 is 90:5:5 or 95:2.5:2.5.

[0031] Preferably, in the composition, the mass ratio of ophiorrhiza japonica thunb polysaccharide, ginsenoside Rb1 and ginsenoside Rg1 is preferably 90:5:5.

[0032] Preferably, the laying performance refers to the laying rate, and the egg quality refers to the egg weight and the eggshell strength.

[0033] The beneficial effects of the present invention are as follows: Through the reasonable ratio of polysaccharide from Ophiopogon japonicus (Thunb.) Ker-Gawl. roots to ginsenoside Rb1 and Rg1, the composition of the present invention significantly enhances the intestinal digestive enzyme activities of laying hens, especially the activities of trypsin and amylase. The enhanced digestive enzyme activities contribute to improving the feed digestion and absorption efficiency of laying hens, and promoting laying hens to more efficiently absorb the nutritional components in the feed.

[0034] Meanwhile, experimental data show that feeding the feed of the present invention can significantly increase the egg production rate of laying hens and reduce feed waste. Due to the improvement of digestive enzyme activities, laying hens can more effectively utilize the nutrients in the feed, thereby increasing the egg production.

[0035] Secondly, the use of the composition can not only increase the egg production rate, but also significantly improve the egg weight and eggshell strength, helping laying hens produce more and better-quality eggs to meet the market demand for high-quality egg products. Brief Description of the Drawings

[0036] Figure 1 are the results of the differences in digestive enzyme activities of laying hens in different experimental groups;

[0037] Among them, Figure 1 (a) are the results of the differences in trypsin, Figure 1 (b) are the results of the differences in lipase, Figure 1 (c) are the results of the differences in amylase;

[0038] Figure 2 are the results of the differences in egg production rates of laying hens in different experimental groups;

[0039] Figure 3 are the results of the differences in egg qualities of laying hens in different experimental groups;

[0040] Among them, Figure 3 (a) are the results of the differences in egg weights, Figure 3 (b) are the results of the differences in eggshell strengths. Detailed Embodiments

[0041] Next, the optional embodiments of the present invention application will be elaborated in more detail. Although the optional embodiments of the present invention application have been described, it should be clear that the present invention application can be implemented in various forms and should not be restricted by the embodiments listed here. In fact, these embodiments are provided to make the content of the present invention application clearer and more comprehensive, so as to ensure that those skilled in the art can accurately and completely understand the scope of the present invention application.

[0042] In the application of the present invention, the terms used are only for describing specific embodiments and are not intended to limit the application of the present invention. Moreover, in the present application and the appended claims, the singular forms "a", "the", and "said" used, in the absence of context clearly indicating other meanings, also cover plural forms. In addition, it should also be understood that the term "and / or" appearing in the text refers to any or all possible combinations including one or more of the associated listed items.

[0043] Example 1

[0044] (1) After washing the dried Diploclisia glaucescens (tuber) 3 times with clear water, the Diploclisia glaucescens was pulverized into fine powder with a pulverizer and passed through an 80-mesh sieve to obtain Diploclisia glaucescens powder;

[0045] (2) To the Diploclisia glaucescens powder, 15 times the amount of deionized water was added, and at the same time, 2.5% cellulase was added, and the reaction was carried out in a water bath at 50 °C for 60 minutes;

[0046] (3) After inactivating at 90 °C for 10 minutes, ultrasonic extraction was carried out at a power of 250 W and a temperature of 60 °C for 45 min;

[0047] (4) Centrifugation was carried out at 4000 rpm for 15 min, the supernatant was collected, activated carbon was added according to a solid-liquid ratio of 5:100, and stirring and decolorization were carried out for 30 minutes;

[0048] (5) The filtrate was collected by filtration, the filtrate was concentrated to 1 / 3 of the original volume, and the same volume of sevage reagent was added to remove protein 5 times to obtain a crude polysaccharide solution of Diploclisia glaucescens;

[0049] (6) 3 times the amount of 95% ethanol cooled to 4 °C was added to the Diploclisia glaucescens solution, and static alcohol precipitation was carried out at 4 °C for 16 hours;

[0050] (7) Centrifugation was carried out at 4000 rpm for 20 minutes, the precipitate was collected, washed 2 times with pre-cooled 95% ethanol, and then washed 2 times with pre-cooled acetone, and dried at room temperature to obtain crude polysaccharide of Diploclisia glaucescens;

[0051] (8) The crude polysaccharide extract of Diploclisia glaucescens was dissolved with 10 times the amount of water and filtered through a 50 kDa ultrafiltration membrane, and the permeate was collected;

[0052] (9) The permeate was filtered through a 10 kDa ultrafiltration membrane, and the retentate was collected to obtain a polysaccharide extract of Diploclisia glaucescens;

[0053] (10) After being concentrated under reduced pressure to 1 / 3 of the original volume, it was freeze-dried in a freeze dryer to obtain polysaccharide of Diploclisia glaucescens.

[0054] Example 2

[0055] The formula of laying hen feed 1 consists of 99.95% basic diet and 0.05% of composition 1;

[0056] The basic diet is composed of 67.00%, 3.00% wheat bran, 14.00% soybean meal, 5% cottonseed meal, 4% rapeseed meal, 2.00% meat floss powder, 4.00% limestone powder and 1.00% premix.

[0057] The premix provides per kilogram of diet: VA 10000 IU, VD 3 2500 IU, VE 18 IU, VK 3 1 mg, VB 1 2 mg, VB 2 6 mg, VB 6 3.5 mg, VB 12 15 μg, pantothenic acid 18 mg, niacin 30 mg, biotin 0.15 mg, folic acid 0.4 mg, Fe 80 mg, Cu 9 mg, Zn 70 mg, Mn 80 mg, I 0.6 mg, Se 0.3 mg.

[0058] The composition consists of ophiorrhiza polysaccharide, ginsenoside Rb1 and Rg1, and the mass ratio of ophiorrhiza polysaccharide, ginsenoside Rb1 and Rg1 is 90:5:5.

[0059] Example 3

[0060] The formula of laying hen feed 2 consists of 99.95% basic diet and 0.05% of composition 2;

[0061] The components of the basic diet are the same as those in Example 2;

[0062] Composition 2 consists of ophiorrhiza polysaccharide, ginsenoside Rb1 and Rg1, and the mass ratio of ophiorrhiza polysaccharide, ginsenoside Rb1 and Rg1 is 95:2.5:2.5.

[0063] Comparative Example 1

[0064] The formula of laying hen feed 3 consists of 99.95% basic diet and 0.05% of ophiorrhiza polysaccharide.

[0065] Comparative Example 2

[0066] The formula of laying hen feed 4 consists of 99.95% basic diet and 0.05% of composition 3;

[0067] The components of the basic diet are the same as those in Example 2;

[0068] Composition 3 consists of ganoderma lucidum polysaccharide, ginsenoside Rb1 and Rg1, and the mass ratio of ganoderma lucidum polysaccharide, ginsenoside Rb1 and Rg1 is 90:5:5.

[0069] Comparative Example 3

[0070] The formula of laying hen feed 5 consists of 99.95% of the basal diet and 0.05% of Composition 4;

[0071] The components of the basal diet are the same as those in Example 2;

[0072] Composition 4 consists of ophiorrhiza japonica polysaccharide and ginsenoside Rb1, and the mass ratio of ophiorrhiza japonica polysaccharide to ginsenoside Rb1 is 90:10.

[0073] Comparative Example 4

[0074] The formula of laying hen feed 6 consists of 99.95% of the basal diet and 0.05% of Composition 5;

[0075] The components of the basal diet are the same as those in Example 2;

[0076] Composition 5 consists of ophiorrhiza japonica polysaccharide and ginsenoside Rg1, and the mass ratio of ophiorrhiza japonica polysaccharide to ginsenoside Rg1 is 90:10.

[0077] Example 4

[0078] (1) Select 56-week-old Hy-Line Brown laying hens with healthy and consistent egg-laying performance, and randomly divide them into 7 groups (control group and experimental groups a-f), with 3 replicates in each group and 20 hens in each replicate;

[0079] (2) The control group was fed the basal diet, experimental group a was fed laying hen feed 1, experimental group b was fed laying hen feed 2, experimental group c was fed laying hen feed 3, experimental group d was fed laying hen feed 4, and experimental group e was fed laying hen feed 5;

[0080] (3) The laying hens were raised individually in cages, and a water dispenser was set in each cage. During the experiment, the temperature was set at 25 °C, the relative humidity was 65%, and the lighting was 6 hours / 8 hours of darkness;

[0081] (4) The pre-experiment lasted for 1 week for the laying hens to adapt to the environment and feed, and the formal experiment lasted for 6 weeks.

[0082] Example 5

[0083] (1) On the day when the experiment ended, 5 laying hens were randomly selected from each replicate, sacrificed and dissected, and the duodenum was taken out;

[0084] (2) Gently squeeze the intestinal segment, and collect the contents into a pre-cooled sterile centrifuge tube;

[0085] (3) Dilute the duodenal contents 1:10 with 0.1% normal saline on ice, and homogenize with a sterile homogenizer to obtain a homogenate;

[0086] (4) Centrifuge at 4°C and 12,000 r / min for 15 min, collect the supernatant, and use an ELISA kit (Nanjing Jiancheng Bioengineering Co., Ltd.) to detect the activity differences of trypsin, lipase, and amylase. The results are shown in Table 1 and Figure 1 as follows.

[0087] Table 1 Activity differences of digestive enzymes in the duodenum of laying hens in different experimental groups

[0088]

[0089] According to the results in Table 1, it can be seen that the trypsin activity and amylase activity of experimental group a and experimental group b are significantly higher than those of other experimental groups and the control group, indicating that the digestive enzyme activity of laying hens fed with laying hen feed 1 and laying hen feed 2 provided in Example 2 and Example 3 of the present invention has been significantly improved. Among them, the trypsin activity and amylase activity of laying hens in experimental group a are significantly higher than those in experimental group b, indicating that when combining dioscorea bulbifera polysaccharide, ginsenoside Rb1, and Rg1 according to the formula of laying hen feed 1, the synergistic effect of the three in promoting the protease activity and amylase activity of laying hens can be fully exerted.

[0090] Further observation shows that the trypsin activity and amylase activity of experimental group c are somewhat higher than those of the control group, but the effect is not significant enough. This indicates that using only laying hen feed 3 containing dioscorea bulbifera polysaccharide can promote the activities of trypsin and amylase to a certain extent, but the effect is relatively limited and fails to achieve the expected significant improvement. This suggests that the strategy of single addition of functional polysaccharides in the feed formula is difficult to fully exert its potential.

[0091] The trypsin activity and amylase activity of experimental group d are the lowest among all experimental groups, indicating that when replacing dioscorea bulbifera polysaccharide with ganoderma lucidum polysaccharide, even when combining ganoderma lucidum polysaccharide with ginsenoside Rb1 and Rg1 in a timely manner, the synergistic effect of promoting the digestive enzyme activity of laying hens cannot be exerted. This may be because ganoderma lucidum polysaccharide fails to form an effective synergistic effect with ginsenoside Rb1 and Rg1 under the experimental conditions and cannot replace the function of dioscorea bulbifera polysaccharide in enhancing digestive enzyme activity.

[0092] In addition, the results of experimental group e and experimental group f further illustrate that when using ginsenoside Rb1 or ginsenoside Rg1 alone in combination with dioscorea bulbifera polysaccharide, although the effect is better than that of laying hen feed only added with dioscorea bulbifera polysaccharide, there are still very significant differences compared with the combined effect of adding ginsenoside Rb1 and Rg1 simultaneously. This indicates that relying solely on the combined use of a single ginsenoside (Rb1 or Rg1) and dioscorea bulbifera polysaccharide cannot fully exert the synergistic effect. Therefore, the combined use of ginsenoside Rb1 and Rg1 is the key condition for achieving synergistic improvement of the digestive enzyme activity of laying hens with dioscorea bulbifera polysaccharide.

[0093] Example 6

[0094] (1) During the whole experiment, the number of eggs laid by laying hens was collected daily and the egg production rate was calculated. The detailed egg production rate results are shown in Table 2 and Figure 2 as follows.

[0095] (2) The egg weight and eggshell strength were detected. The results are shown in Table 3 and Figure 3 as follows.

[0096] Table 2 Differences in egg production rate of laying hens in different experimental groups

[0097] Detection index Control group Experimental group a Experimental group b Experimental group c Experimental group d Experimental group e Experimental group f Egg production rate / % 85.33±1.28 93.99±1.53 91.11±1.36 87.18±1.21 86.13±1.32 88.72±1.31 88.87±1.54

[0098] Table 3 Differences in egg quality of laying hens in different experimental groups

[0099] Detection index Control group Experimental group a Experimental group b Experimental group c Experimental group d Experimental group e Experimental group f Egg weight / g 61.47±0.46 67.02±0.60 65.12±0.71 62.20±0.51 61.71±0.69 62.71±0.39 63.17±0.56 Eggshell strength / kgf .129±0.045 .674±0.062 .505±0.043 3.203±0.035 .199±0.029 .284±0.044 3.328±0.028

[0100] Table 2 shows the differences in egg production rate of laying hens in different experimental groups. The egg production rate of the control group was 85.33%, while those of experimental groups a and b were 93.99% and 91.11% respectively, which were significantly higher than those of the control group and other experimental groups. The egg production rate of experimental group c was 87.18%, slightly higher than that of the control group, but the difference was not significant. The egg production rate of experimental group d was only 86.13%, similar to that of the control group, indicating that Ganoderma lucidum polysaccharide replacing Diplazium esculentum polysaccharide had limited effect on the egg production rate. The egg production rates of experimental groups e and f were 88.72% and 88.87% respectively, both higher than that of the control group, but lower than those of experimental groups a and b.

[0101] These results further verified the findings in Table 1, that is, the combination of Diplazium esculentum polysaccharide, ginsenoside Rb1 and Rg1 significantly increased the egg production rate of laying hens. The egg production rate of experimental group a was the highest, indicating that according to the formula of laying hen feed 1, the combination of Diplazium esculentum polysaccharide, ginsenoside Rb1 and Rg1 could effectively promote the physiological function of laying hens and improve the egg production efficiency.

[0102] Table 3 shows the differences in egg weight and eggshell strength of laying hens in different experimental groups. The egg weight of the control group was 61.47 g and the eggshell strength was 3.129 kgf. The egg weight and eggshell strength of experimental group a were 67.02 g and 3.674 kgf respectively, which were significantly higher than those of the control group. The egg weight of experimental group b was 65.12 g and the eggshell strength was 3.505 kgf, also significantly better than those of the control group. The egg weight and eggshell strength of experimental group c were 62.20 g and 3.203 kgf respectively, slightly higher than those of the control group but not significantly. The egg weight of experimental group d was 61.71 g and the eggshell strength was 3.199 kgf, similar to those of the control group. The egg weights of experimental groups e and f were 62.71 g and 63.17 g respectively, and the eggshell strengths were 3.284 kgf and 3.328 kgf respectively, both better than those of the control group, but lower than those of experimental groups a and b.

[0103] These data indicate that the combination of polysaccharide from Ophiopogon bodinieri Levl. and ginsenosides Rb1 and Rg1 not only improves the digestive enzyme activity and egg production rate of laying hens, but also significantly improves the egg quality of laying hens, specifically manifested as the increase in egg weight and eggshell strength. This further proves the effectiveness of the synergistic effect of the three, enhancing the overall production performance of laying hens.

Claims

1. A composition for improving the activity of digestive enzymes in the intestinal tract of laying hens, characterized in that: The composition consists of nephrodisiac polysaccharide, ginsenoside Rb1 and ginsenoside Rg1.

2. The composition according to claim 1, characterized in that The double kidney polysaccharide is prepared according to the following preparation method: (1) Wash and crush the dried kidney, and mix it with water at a solid-to-liquid ratio of 1:15; (2) After adding cellulase for enzymolysis, the mixture was inactivated by heating and ultrasonic extraction was performed at an ultrasonic power of 250 W, a temperature of 60 ° C, and a time of 45 min; (3) After collecting the supernatant by centrifugation, decolorization is performed using activated carbon; (4) collecting the filtrate by filtration, concentrating it, and removing the protein using a sevage reagent to obtain a crude polysaccharide solution of the nephrodisiac; (5) adding 3 times the amount of ethanol for alcohol precipitation, collecting the precipitate by centrifugation and washing to obtain crude polysaccharide of nephrodisiac; (6) After dissolving the crude nephrodisiac polysaccharide, using an ultrafiltration membrane to collect 10 kDa-50 kDa nephrodisiac polysaccharide solution; (7) After concentration, freeze-drying is performed to obtain the polysaccharide of the nephrodisiac.

3. The composition according to claim 1, characterized in that In the composition, the mass ratio of nephrodisiac polysaccharide, ginsenoside Rb1 and ginsenoside Rg1 is 90:5:5 or 95:2.5:2.

5.

4. The composition according to claim 1, characterized in that The intestinal digestive enzymes are duodenal trypsin and amylase.

5. A feed for improving the activity of digestive enzymes in the intestinal tract of laying hens, characterized in that: The feed comprises a basic diet for laying hens and the composition according to any one of claims 1 to 4, and the added amount of the composition in the feed is greater than or equal to 0.05%.

6. Use of the composition according to any one of claims 1 to 4 in preparing a feed for improving the activity of intestinal digestive enzymes in laying hens, characterized in that: The intestinal digestive enzymes are duodenal trypsin and amylase.

7. A composition for improving the egg-laying performance and egg-laying quality of laying hens, characterized in that: The composition consists of nephrodisiac polysaccharide, ginsenoside Rb1 and ginsenoside Rg1. In the composition, the mass ratio of nephrodisiac polysaccharide, ginsenoside Rb1 and ginsenoside Rg1 is 90:5:5 or 95:2.5:2.

5.

8. The composition according to claim 7, characterized in that The egg-laying performance refers to the egg-laying rate, and the egg-laying quality refers to the egg weight and eggshell strength; The bile syrup polysaccharide is prepared according to the preparation method described in claim 2.

9. A feed for improving the egg-laying performance and egg-laying quality of laying hens, characterized in that: The feed comprises a basic diet for laying hens and the composition according to claim 7 or 8, and the added amount of the composition in the feed is greater than or equal to 0.05%.

10. The feed according to claim 9, characterized in that The egg-laying performance refers to the egg-laying rate, and the egg-laying quality refers to the egg weight and the eggshell strength.

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