Anti-cancer selenium-enriched soft-shelled turtle soup medicated diet and making method thereof
By using selenium-rich turtle and low-temperature staged stewing process to extract anti-cancer ingredients, the problems of low selenium content and poor ingredient synergy in existing anti-cancer medicinal diets are solved, and efficient anti-cancer and immune regulation effects are achieved, and the safety is also in line with food safety standards.
Patent Information
- Application Number
- CN202510345314.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-24
- Publication Date
- 2025-06-10
AI Technical Summary
In the existing anti-cancer medicinal diet, ordinary turtles have low selenium content and poor synergies of ingredients. Stewing at high temperatures leads to degradation of heat-sensitive ingredients, making it difficult to effectively inhibit tumor cells.
Selenium-rich turtle is used as the core raw material, and Ganoderma lucidum triterpenes and astragalus polysaccharides are extracted through a low-temperature phased stewing process, and it is coordinated with Chinese herbal medicines such as yam and wolfberry to form a multi-target anti-cancer mechanism.
The anti-cancer effect of high selenium content and multiple active ingredients was achieved. In vitro experiments showed that the inhibition rate of liver cancer cells reached 62.5%, and animal experiments showed that the tumor volume was reduced by 54.3%, and the immune function was significantly improved in clinical trials, and the safety was in line with food safety standards.
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of functional medicated diets, and specifically relates to a medicated diet formula and preparation process that uses selenium-rich soft-shelled turtles as the core raw material and has the effects of preventing and treating cancer and regulating immunity. Background Art
[0002] Existing anti-cancer medicated diets mostly use ordinary soft-shelled turtles or single Chinese medicinal materials (such as Ganoderma lucidum) in combination, and there are the following problems: 1. Insufficient selenium content: The selenium content of ordinary soft-shelled turtles is low (usually ≤0.1 mg / kg), making it difficult to reach the dose threshold for inhibiting tumor cells; 2. Poor component synergy: Traditional formulas lack the synergistic design of selenium with polysaccharides and triterpenoids; 3. Process destroys activity: High-temperature long-term stewing causes the degradation of heat-sensitive components such as Ganoderma lucidum triterpenoids and selenium proteins. Summary of the Invention
[0003] Object of the Invention To provide an anti-cancer medicated diet with high selenium content and synergistic multi-active ingredients, and through optimizing the formula and process, achieve the synergistic compatibility of selenium and Chinese medicinal materials. Technical Solution
[0004] 1. Innovation of the core formula: Selenium-rich soft-shelled turtle: Raised with selenium-rich feed, the main selenium form in its liver and muscle is selenocysteine, with high bioavailability; Synergy of active ingredients: The combination of Ganoderma lucidum (Ganoderma lucidum triterpenoids) and selenium can activate the Nrf2 / ARE antioxidant pathway, and astragalus polysaccharide enhances the activity of NK cells, forming a multi-target anti-cancer mechanism.
[0005] 2. Process innovation: Low-temperature staged stewing: In the first stage, extract Ganoderma lucidum triterpenoids and astragalus polysaccharide at low temperature (60 - 80°C); in the second stage, add Chinese yam and wolfberry to avoid the loss of antioxidant components such as vitamin C; Pretreatment to remove fishy smell: After blanching the soft-shelled turtle, marinate it with ginger and rice wine for 10 minutes to remove the fishy smell and keep the selenium protein structure intact.
[0006] Beneficial Effects: 1. Verification of anti-cancer activity: In vitro experiments show that the inhibition rate of the extract of this medicated diet on HepG2 liver cancer cells (after 48 hours) reaches 62.5% (IC50 = 85 μg / mL); Animal experiments show that after intragastric administration to tumor-bearing mice for 28 days, the tumor volume is reduced by 54.3% compared with the control group (P < 0.01).
[0007] 2. Immune regulation: In clinical trials, after 30 radiotherapy and chemotherapy patients continuously took the medicine for 4 weeks, the CD4+ / CD8+ ratio increased by 1.8 times, and the proportion of white blood cell count restored to the normal level increased by 65%.
[0008] 3. Safety: No liver and kidney damage was found in the acute toxicity test (LD50>15 g / kg) and the long-term toxicity test (90 days), meeting the food safety standards. Specific implementation manners
[0009] Example 1 (basic formula) Raw materials: 600 g of selenium-rich soft-shelled turtle (selenium content 0.6 mg / kg), 15 g of Ganoderma lucidum, 20 g of Astragalus membranaceus, 15 g of Lycium barbarum, 80 g of Chinese yam, 30 g of Poria cocos, 12 g of ginger, 2500 ml of clear water, 4 g of salt.
[0010] Steps: 1. After blanching the soft-shelled turtle, cook it together with Ganoderma lucidum (pre-soaked), Astragalus membranaceus, Poria cocos, and ginger at 80°C for 2 hours; 2. Add Chinese yam and cook for 35 minutes, and add Lycium barbarum and salt 10 minutes before turning off the heat.
[0011] Example 2 (extended formula) On the basis of Example 1, add 8 g of American ginseng and 30 g of coix seed, which is applicable to tumor patients with damp-heat constitution.
Claims
1. A medicinal diet rich in selenium turtle soup for cancer prevention and treatment, characterized in that: It includes the following raw materials in parts by weight: 1 selenium-rich turtle (500-800g) Lingzhi 10-20g Astragalus 15-30g 10-20g wolfberry 50-100g Chinese Yam Poria 20-40g 10-15g ginger 2000-3000ml water 3-6g salt.
2. The medicated diet according to claim 1, characterized in that: The selenium-rich turtle is an artificially bred selenium-rich turtle, and its selenium content is ≥0.5 mg / kg (based on dry weight).
3. The medicated diet according to claim 1, characterized in that: Also includes at least one of the following optional ingredients: American ginseng 5-10g Coix seeds 20-40g Red dates 10-20g 10-20g lily.
4. A method for preparing the selenium-enriched turtle soup medicinal diet according to any one of claims 1 to 3, characterized in that: The following steps are involved: (1) Pretreatment: Kill the turtle, remove the internal organs, blanch it in boiling water for 2-3 minutes to remove the epidermis and mucous membrane, and cut it into pieces for later use; (2) First stew: Add soft-shell turtle, ganoderma, astragalus, poria, and ginger to water, bring to a boil over high heat, then simmer at 60-80℃ for 1.5-2 hours; (3) Second stew: Add yam and wolfberry and continue stewing for 30-40 minutes. Add salt to taste.
5. The method according to claim 4, characterized in that In step (2), the Ganoderma lucidum needs to be soaked in 40-50°C warm water for 30 minutes in advance to activate the spore activity.
6. The method according to claim 4, characterized in that The amount of salt added in step (3) is 0.1%-0.3% (w / v) of the total soup.