Improving agent for improving artificial breeding survival rate of plectropomus latifolius and application of improving agent

Abstract

The invention discloses a modifier for improving the artificial breeding survival rate of red plectropomus latus and application, and belongs to the technical field of fish culture. The modifier is prepared from the following components in parts by weight: 15 to 20 parts of traditional Chinese medicine probiotic fermentation liquor, 8 to 10 parts of herba portulacae, 8 to 10 parts of radix rehmanniae, 5 to 8 parts of cypress essential oil and 5 to 8 parts of phenylalanine, the traditional Chinese medicine probiotic fermentation liquor is obtained by fermenting evening primrose, exocarpium citri grandis and tussilago farfara through lactobacillus reuteri. Through verification, by using the modifier prepared by the invention, the survival rate of artificially bred young pterus salmoides is 96% or above; by measuring the body weight and the overall length of the red nine-spiny perch fry in the breeding period, it is found that the fry obtained by using the modifier is neat in specification and more consistent in food grabbing and growth speed, possibly due to the fact that the competition and survival pressure caused by individual differences is reduced, and the overall survival rate is increased. Therefore, the invention provides a new thought and technical support for improving the survival rate of the red pink bass.

Description

Technical Field

[0001] The present invention relates to the technical field of fish farming, and particularly to an improver for improving the survival rate of artificial breeding of Cephalopholis sonnerati and its application. Background Art

[0002] Cephalopholis sonnerati, commonly known as reticulated rockcod, crosshatch rockcod, coral hind, red grouper, red jewfish, is a fish of the genus Cephalopholis in the family Serranidae, suborder Percoidei, order Perciformes, class Actinopterygii. The body is red, with dark brown reticulations on the head and the front of the back, without spots and stripes. There are no canines in the middle of the lower jaw, and the anal fin has 3 fin spines and 9 fin rays. The trailing edge of the caudal fin is round, without stripes. It often inhabits the shallow sea with rocky bottoms, feeding on invertebrates and fish, and is a tropical coral reef and coastal fish. As a high-grade edible fish in the existing market, Cephalopholis sonnerati is deeply loved by consumers. At present, the population of wild Cephalopholis sonnerati has decreased significantly due to overfishing. However, the current artificial breeding technology of Cephalopholis sonnerati is not yet mature, and there is little research on the artificial breeding method of Cephalopholis sonnerati fry. It is not possible to carry out large-scale artificial breeding of Cephalopholis sonnerati fry. Especially during the breeding process, the feeding rate of the fry is unstable, resulting in low growth and development, low reproductive ability, and low survival rate of the fry, unable to meet the market demand for Cephalopholis sonnerati.

[0003] The control of the ecological environment of aquaculture water bodies is the key to the sustainable and healthy development of aquaculture. The quality of the water quality and sediment in the aquaculture water bodies determines the ecological environment quality of the aquaculture water bodies. In the aquaculture water bodies, the water quality is the intuitive manifestation of the water body environment quality. Some soluble substances and gases affect the water quality, while the water body sediment is the root of the water body environment quality. With the development of the aquaculture industry, the aquaculture methods pursuing high yield and high efficiency have brought unprecedented ecological pressure to the water body sediment. With the development of aquaculture, high-density aquaculture requires a large amount of feed input. The residual bait and the excrement of aquatic animals pollute the aquaculture water bodies, resulting in an increase in harmful substances such as NH 3 、H 2 S, organic matter, etc., an increase in chemical oxygen demand (COD) and biological oxygen demand (BOD), seriously affecting the growth of aquatic animals. It causes osmotic imbalance, stress, and decreased immunity in the cultured animals, making them prone to outbreak of pathogenic bacteria infection and death, resulting in aquaculture failure. In the past, a large number of aquaculture farmers used antibiotics for prevention and treatment. The abuse of antibiotics has accelerated the deterioration of water quality, causing secondary stress to the cultured animals and bringing greater difficulties to disease prevention and treatment. It is easy to cause outbreak of death of the cultured animals and affect the quality of the cultured animals. Exports are affected, causing indirect harm to the human body. Therefore, timely regulation of water quality and increasing the aquaculture success rate have become problems for the sustainable development of the aquaculture industry. Summary of the Invention

[0004] The object of the present invention is to provide an improver for improving the survival rate of artificial breeding of Cephalopholis sonnerati and its application to solve the problems existing in the above-mentioned prior art. By fermenting evening primrose, tangerine peel and coltsfoot flower with Lactobacillus reuteri and then mixing them with purslane, rehmannia root, cypress essential oil and phenylalanine, the improver for improving the survival rate of artificial breeding of Cephalopholis sonnerati is prepared. The preparation process is simple and easy to operate. It has been verified that by using the improver prepared by the present invention, the survival rate of the artificially bred Cephalopholis sonnerati seedlings is above 96%, and the obtained fry have uniform specifications, making them more consistent in food competition and growth rate, reducing competition and survival pressure caused by individual differences, thereby improving the overall survival rate.

[0005] To achieve the above object, the present invention provides the following solutions:

[0006] The present invention provides an improver for improving the survival rate of artificial breeding of Cephalopholis sonnerati, which comprises the following components by weight:

[0007] 15-20 parts of traditional Chinese medicine probiotic fermentation broth, 8-10 parts of purslane, 8-10 parts of rehmannia root, 5-8 parts of cypress essential oil and 5-8 parts of phenylalanine;

[0008] The traditional Chinese medicine probiotic fermentation broth is obtained by fermenting evening primrose, tangerine peel and coltsfoot flower with Lactobacillus reuteri.

[0009] Preferably, the weight ratio of evening primrose, tangerine peel and coltsfoot flower is (5-7):(3-5):(2-3); the strain number of Lactobacillus reuteri is CICC 6119.

[0010] The present invention also provides a preparation method of the improver, which comprises the following steps:

[0011] Take 5-7 parts of evening primrose, 3-5 parts of tangerine peel and 2-3 parts of coltsfoot flower, mix and ultrafinely pulverize to obtain ultrafine powder, soak in water and grind to obtain slurry; inoculate Lactobacillus reuteri liquid into the slurry, ferment, centrifuge and take the supernatant to obtain traditional Chinese medicine probiotic fermentation broth;

[0012] Take purslane and rehmannia root, dry and pulverize them into ultrafine powder to obtain a mixture powder; add water to the mixture powder, decoct and filter to obtain decoction A and medicinal residues; add water to the medicinal residues, decoct and filter again to obtain decoction B; combine decoction A and decoction B to obtain traditional Chinese medicine extract;

[0013] Mix the traditional Chinese medicine probiotic fermentation broth, traditional Chinese medicine extract, cypress essential oil and phenylalanine to obtain the improver.

[0014] Optionally, the volume ratio of the ultrafine powder to water is 1:(8-10);

[0015] The inoculation amount of the Lactobacillus reuteri bacterial liquid is 5%, and the concentration of the Lactobacillus reuteri bacterial liquid is 4×10 9 cfu / mL.

[0016] Optionally, the fermentation conditions are a pH of 7, a temperature of 39°C, and a fermentation time of 28 hours.

[0017] Optionally, the volume ratio of the mixture powder to water is 1:(20 - 25); the decocting time is 80 min - 90 min.

[0018] Optionally, the volume ratio of the medicinal residues to water is 1:(7 - 9); the time for re - decocting is 50 min - 70 min.

[0019] The present invention also provides the application of the said modifier in improving the survival rate of artificial breeding of Cephalopholis sonnerati.

[0020] Optionally, the dosage of the modifier is 10 - 20 mL / m 2 .

[0021] The present invention also provides the application of the said modifier in the preparation of a product for improving the survival rate of artificial breeding of Cephalopholis sonnerati.

[0022] The present invention discloses the following technical effects:

[0023] After fermenting evening primrose, tangerine peel, and coltsfoot flower with Lactobacillus reuteri, and then mixing them with purslane, rehmannia root, cypress essential oil, and phenylalanine to prepare a modifier for improving the survival rate of artificial breeding of Cephalopholis sonnerati, the preparation process is simple and easy to operate; through experimental verification, by using the modifier prepared by the present invention, the survival rate of artificially bred Cephalopholis sonnerati fry is above 96%. By measuring the body weight and total length of the Cephalopholis sonnerati fry during the breeding period, it is found that the fry sizes obtained by using the modifier prepared by the present invention are uniform, making them more consistent in food competition and growth rate. This may be due to reducing competition and survival pressure caused by individual differences, thereby improving the overall survival rate. It can be seen that the present invention provides new ideas and technical support for improving the survival rate of Cephalopholis sonnerati. Detailed Embodiments

[0024] The various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, characteristics, and implementation schemes of the present invention.

[0025] It should be understood that the terms described in the present invention are only for describing particular embodiments and are not intended to limit the present invention. Additionally, for the numerical ranges in the present invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Any intermediate value within any stated value or stated range, as well as each smaller range between any other stated value or intermediate value within the stated range, is also included in the present invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0026] Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Although the present invention only describes preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials related to the documents. In case of conflict with any incorporated document, the content of this specification shall prevail.

[0027] Without departing from the scope or spirit of the present invention, various improvements and changes can be made to the specific embodiments of the present invention specification, which are obvious to those skilled in the art. Other embodiments obtained from the present invention specification are obvious to those skilled in the art. The present invention specification and examples are merely exemplary.

[0028] Regarding the use of "comprising", "including", "having", "containing", etc. herein, they are all open-ended terms, meaning including but not limited to.

[0029] In the prior art, a variety of technical solutions for improving the survival rate of red grouper fry have been disclosed. For example, in Patent CN118901632A - A Cultivation Method for High Survival Rate of Red Grouper Fry, it includes: (1) Regulating the light source environment: During the growth process of red grouper fry, irradiate with a blue light source with a wavelength range of 420 - 450 nm for 60 - 75 days; (2) Transforming the bottom environment: Lay a layer of artificial material on the bottom, introduce water with a salinity of 30 - 35‰ as the aquaculture water body, and use an aerator to aerate throughout the day; (3) Alternately feeding Feed A and Feed B: First feed Feed A for 8 - 10 days, then feed Feed A for 8 - 10 days, and alternate feeding until the juvenile fish stage; Feed A is prepared by adding Daphniphyllum calycinum to the basic feed, and Feed B is prepared by adding loperamide to the basic feed. By means of these three parallel measures of regulating the light source + transforming the bottom + alternate feeding, it is beneficial to improve the high survival rate of red grouper fry, thereby increasing economic benefits. This patent application uses a blue light source with a specific spectrum to regulate the light environment of red grouper fry, meets the light requirements of red grouper, improves the problem of uneven specifications during the growth process of red grouper, makes the growth rate of red grouper not vary much within the same time, and effectively improves the economic benefits of red grouper aquaculture. This patent application uses cetyltrimethoxysilane as a modifier to hydrophobically modify oyster shell powder - kaolin to improve adsorption, and synergistically purifies the water body with sodium silicate, superphosphate, and the metabolite of Clostridium butyricum, improves the water environment, and is beneficial to improving the survival rate of red grouper fry; moreover, the metabolite of Clostridium butyricum promotes the growth of probiotics in the fry intestine, inhibits harmful bacteria, corrects the disorder of the flora, reduces toxin production, regulates the microecological balance of the fish intestine, and thus shortens the breeding time of red grouper fry and reduces the breeding cost. This patent application alternately feeds Feed A and Feed B. Feed A contains traditional Chinese medicine, and Feed B contains Western medicine. The components of traditional Chinese medicine are consistent with the activity of the fish body, can be fully absorbed and utilized by the body, and will not produce drug residues in the body at all, avoiding the problem of drug residues; while Western medicine takes effect quickly and can quickly promote the fish body to digest food and absorb nutrients. The combined application of traditional Chinese medicine and Western medicine can produce a synergistic effect, regulate the body's immunity, and improve the problem of low yield of red grouper. Although the method provided by this patent can improve the survival rate of red grouper fry, it is complex in operation, involves multiple operations, and is not easy to popularize.

[0030] Patent CN109349173A - Cultivation method for improving the survival rate of grouper fry. A cultivation method for improving the survival rate of grouper fry is provided, including indoor seedling cultivation and outdoor seedling cultivation. Indoor seedling cultivation specifically involves hatching grouper fertilized eggs in an indoor seedling cultivation pool and cultivating for 3 days to obtain fry. Outdoor seedling cultivation specifically includes the following steps: S1. Inject water into an outdoor seedling cultivation pool with a sunshade shed above to a depth of 7.5 - 8 cm, and add a bactericidal liquid with a quantity of 0.005 times the total mass of water to the outdoor seedling cultivation pool. Then transfer the fry to the outdoor seedling cultivation pool and cultivate for 3 days. Among them, the bactericidal liquid includes the following raw materials in parts by weight: 3 parts of table salt, 2.5 parts of sodium citrate, 2 parts of antioxidant, 30 parts of protease, 50 parts of traditional Chinese medicine residue extract, 7 parts of compound vitamin, and 20 parts of water. S2. Inject water into the outdoor seedling cultivation pool to a depth of 1.5 m and cultivate for 10 days. S3. Remove the sunshade shed on the outdoor seedling cultivation pool and continue to cultivate for 4 days to obtain seedlings. This patent discloses that by using the bactericidal liquid and modified animal bait, the growth of bacteria in the outdoor seedling cultivation pool can be effectively inhibited, and the survival rate of grouper fry can be improved. The traditional Chinese medicine residue extract, in combination with sodium citrate, protease, table salt, and compound vitamin, can effectively inhibit the growth of bacteria, improve the water quality, and reduce the incidence of vibriosis in grouper fry, playing a role in improving the survival rate of grouper fry. Among them, the content of the bactericidal liquid in S1 is high, which can not only sterilize and disinfect the fry but also the traditional Chinese medicine residue extract can improve the stress resistance of the fry. Using a mixed solution composed of table salt, acetic acid, water, and methanol to extract betel nut residue, mint residue, bee hive residue, mulberry leaf residue, and willow branch residue compared with extracting after mixing betel nut, betel nut, bee hive, mulberry leaf, and willow branch with water, the traditional Chinese medicine residue extract can effectively reduce the incidence of vibriosis and play a role in improving the survival rate of grouper fry. The modified animal bait used is added with egg yolk, cicada skin, leech powder, lotus root powder, and orange powder, which can not only promote the metabolism, digestion, and regulate the peristalsis of the intestines and stomach of grouper fry, thereby promoting the growth of grouper fry, shortening the cultivation time of fry, and reducing the bait coefficient. The combined use of the modified animal bait and the bactericidal liquid not only sterilizes the grouper fry and reduces the incidence rate, but also the traditional Chinese medicine residue extract in the bactericidal liquid can promote the digestion and absorption of fly maggot powder, bone meal, and shrimp powder by grouper fry, and further promote the growth of grouper fry and reduce the bait coefficient. The modified animal bait is added with filter residue, which can effectively improve the dispersibility of the modified animal bait, avoid the aggregation of the modified animal bait, make the growth of grouper fry uniform, and at the same time, adding leech powder, orange powder, and lotus root powder can make the fragrance strong, improve the appetite of grouper fry, and further promote the growth of grouper fry. Treating the egg yolk with honey can promote the digestion and absorption of the egg yolk by grouper fry. The defect of this patent application is also that the operation is complex, requiring the combination of indoor and outdoor seedling cultivation and the preparation of the bactericidal liquid, and the control of the operation conditions for multiple steps is relatively strict.Therefore, based on the various methods of improving the survival rate of red nine-spined sea bass fry in the existing technology, but all of them require the combination of multiple stages of operations, and the control of operating conditions is relatively strict. It is still necessary to explore a new, simple and easy method to improve the survival rate of red nine-spined sea bass fry.

[0031] The Lactobacillus reuteri used in the present invention was purchased from the China Industrial Microbiological Culture Collection Center, with the strain number CICC 6119. The phenylalanine used was purchased from Guangdong Hengtian Biotechnology Co., Ltd., CAS: 167088-01-9. The cypress essential oil used was purchased from Guangzhou Hewei Pharmaceutical Technology Co., Ltd., CAS: 8013-86-3.

[0032] Example 1

[0033] An improver for improving the survival rate of artificial breeding of red nine-spined perch, comprising the following components by weight:

[0034] 17 parts of Chinese herbal probiotic fermentation liquid (evening primrose, tangerine peel and coltsfoot flower probiotic fermentation liquid), 9 parts of purslane, 9 parts of rehmannia root, 6 parts of cypress essential oil and 5 parts of phenylalanine.

[0035] The preparation method comprises the following steps:

[0036] (1) Preparation of Chinese herbal probiotic fermentation broth: Lactobacillus reuteri was inoculated into NA medium and cultured anaerobically at 30°C for 18 h to obtain activated Lactobacillus reuteri. Then, 10 wt% of the inoculum was inoculated into MRS medium and cultured anaerobically at 30°C for 48 h to obtain a viable cell count of 4×10 9 cfu / mL of Lactobacillus reuteri liquid. ;

[0037] Take 6 parts of evening primrose, 4 parts of tangerine peel and 2 parts of coltsfoot flower, mix and ultrafine grind to obtain ultrafine powder, add 10 times volume of pure water to soak, grind to obtain slurry;

[0038] Inoculate the slurry with Lactobacillus reuteri liquid at a rate of 5%, ferment at pH = 7, temperature 39° C. for 28 hours, centrifuge at 95° C., and obtain the supernatant to obtain a Chinese medicine probiotic fermentation liquid;

[0039] (2) Take Purslane and Rehmannia root, rinse them thoroughly with pure water, and then dry them until the moisture content is less than 10%; use an ultrafine grinder to fully grind the dried medicinal materials into ultrafine powder to obtain a medicinal mixture powder; add 20 times the volume of pure water to the medicinal mixture powder, heat and boil, boil for 90 minutes, cool and filter to obtain decoction A and medicinal residue. Add 8 times the volume of pure water to the medicinal residue, heat and boil again, boil for 60 minutes, cool and filter to obtain decoction B, and discard the medicinal residue. Combine decoction A and decoction B to obtain a traditional Chinese medicine extract;

[0040] (3) Mix the Chinese herbal medicine probiotic fermentation broth, Chinese herbal medicine extract, cypress essential oil and phenylalanine in proportion to obtain the modifier.

[0041] Example 2

[0042] A modifier for improving the artificial breeding survival rate of Cephalopholis sonnerati, by weight, comprises the following components:

[0043] Chinese herbal medicine probiotic fermentation broth (Oenothera biennis, Exocarpium Citri Grandis and Tussilago farfara probiotic fermentation broth) 15 parts, Portulaca oleracea 10 parts, Rehmannia glutinosa 10 parts, cypress essential oil 5 parts and phenylalanine 8 parts.

[0044] The preparation method comprises the following steps:

[0045] (1) Prepare the Chinese herbal medicine probiotic fermentation broth: Inoculate Lactobacillus reuteri into NA medium and anaerobically culture at 30 °C for 18 h to obtain activated Lactobacillus reuteri. Then inoculate it into MRS medium at an inoculation amount of 10 wt% and anaerobically culture at 30 °C for 48 h to obtain a Lactobacillus reuteri bacterial liquid with a viable count of 4×10 9 cfu / mL of Lactobacillus reuteri bacterial liquid;

[0046] Take 5 parts of Oenothera biennis, 3 parts of Exocarpium Citri Grandis and 2 parts of Tussilago farfara, mix and ultrafinely pulverize to obtain an ultrafine powder, add 8 times the volume of pure water, soak and grind to obtain a slurry;

[0047] Inoculate the Lactobacillus reuteri bacterial liquid into the slurry at an inoculation amount of 5%, and ferment at pH = 7 and a temperature of 39 °C for 28 hours. Centrifuge at 95 °C and take the supernatant to obtain the Chinese herbal medicine probiotic fermentation broth;

[0048] (2) Take Portulaca oleracea and Rehmannia glutinosa, thoroughly rinse them with pure water and then dry them until the moisture content is less than 10%; Thoroughly pulverize the dried medicinal materials into an ultrafine powder with an ultrafine pulverizer to obtain a drug mixture powder; Add 25 times the volume of pure water to the drug mixture powder, heat to boiling, decoct for 90 min, cool and filter to obtain decoction A and medicinal residues. Add 7 times the volume of pure water to the medicinal residues, heat to boiling again, decoct for 50 min, cool and filter to obtain decoction B, and discard the medicinal residues. Combine decoction A and decoction B to obtain the Chinese herbal medicine extract;

[0049] (3) Mix the Chinese herbal medicine probiotic fermentation broth, Chinese herbal medicine extract, cypress essential oil and phenylalanine in proportion to obtain the modifier.

[0050] Example 3

[0051] A modifier for improving the artificial breeding survival rate of Cephalopholis sonnerati, by weight, comprises the following components:

[0052] Chinese herbal medicine probiotic fermentation broth (Oenothera biennis, Exocarpium Citri Grandis and Tussilago farfara probiotic fermentation broth) 20 parts, Portulaca oleracea 8 parts, Rehmannia glutinosa 8 parts, cypress essential oil 8 parts and phenylalanine 6 parts.

[0053] The preparation method comprises the following steps:

[0054] (1) Prepare the traditional Chinese medicine probiotic fermentation broth: Inoculate Lactobacillus reuteri into NA medium, anaerobically culture at 30 °C for 18 h to obtain activated Lactobacillus reuteri. Then inoculate it into MRS medium at an inoculation amount of 10 wt%, and anaerobically culture at 30 °C for 48 h to prepare a Lactobacillus reuteri bacterial solution with a viable count of 4×10 9 cfu / mL;

[0055] Take 7 parts of evening primrose, 4 parts of dried tangerine peel, and 3 parts of coltsfoot flower, mix and ultrafinely pulverize to obtain ultrafine powder, add 10 times the volume of pure water to soak and grind to obtain slurry;

[0056] Inoculate the Lactobacillus reuteri bacterial solution into the slurry at an inoculation amount of 5%, ferment at pH = 7 and a temperature of 39 °C for 28 hours, centrifuge at 95 °C and take the supernatant to obtain the traditional Chinese medicine probiotic fermentation broth;

[0057] (2) Take purslane and rehmannia root, fully rinse them with pure water and then dry them until the water content is less than 10%; fully pulverize the dried medicinal materials into ultrafine powder with an ultrafine pulverizer to obtain a drug mixture powder; add 20 times the volume of pure water to the drug mixture powder, heat to boiling, decoct for 90 min, cool and filter to obtain decoction A and medicinal residues. Add 8 times the volume of pure water to the medicinal residues, heat to boiling again, decoct for 60 min, cool and filter to obtain decoction B, and discard the medicinal residues. Combine decoction A and decoction B to obtain the traditional Chinese medicine extract;

[0058] (3) Mix the traditional Chinese medicine probiotic fermentation broth, the traditional Chinese medicine extract, cypress essential oil and phenylalanine in proportion to obtain the modifier.

[0059] Comparative Example 1

[0060] The difference from Example 1 is only that evening primrose, dried tangerine peel and coltsfoot flower are not subjected to probiotic fermentation, but are decocted together with purslane and rehmannia root to obtain the traditional Chinese medicine extract. Specifically as follows.

[0061] A modifier for improving the artificial breeding survival rate of Cephalopholis sonnerati, calculated by weight, comprises the following components:

[0062] 6 parts of evening primrose, 4 parts of dried tangerine peel, 2 parts of coltsfoot flower, 9 parts of purslane, 9 parts of rehmannia root, 6 parts of cypress essential oil and 5 parts of phenylalanine.

[0063] The preparation method comprises the following steps:

[0064] (1) Take evening primrose, tangerine peel, coltsfoot flower, purslane and rehmannia root, rinse them thoroughly with purified water and then dry them until the moisture content is less than 10%; use an ultrafine pulverizer to fully pulverize the dried medicinal materials into ultrafine powder to obtain a drug mixture powder; add 20 times the volume of purified water to the drug mixture powder, heat to boiling, decoct for 90 minutes, cool and filter to obtain decoction A and medicinal residues. Add 8 times the volume of purified water to the medicinal residues, heat to boiling again, decoct for 60 minutes, cool and filter to obtain decoction B, and discard the medicinal residues. Combine decoction A and decoction B to obtain a traditional Chinese medicine extract;

[0065] (2) Mix the traditional Chinese medicine extract, cypress essential oil and phenylalanine in proportion to obtain a modifier.

[0066] Effect of the application example on the survival rate of red grouper fry

[0067] The experiment was divided into three groups: experimental groups (adding the modifiers prepared in Examples 1-3 respectively), a control group (adding the modifier prepared in Comparative Example 1), and a blank control group (without adding a modifier).

[0068] Parent preparation: Prepare male and female red groupers to be bred, place the parents in a cultivation pond for conventional cultivation, and add the corresponding modifier at 15 mL / m in each cultivation pond. 2 Add the corresponding modifier.

[0069] Parent reproduction: Place the parents in a spawning pond for mating, manage them using conventional management methods, and the fish eggs produced by mating will fall into the spawning pond.

[0070] Hatching into fry: After the eggs hatch into fry, add the corresponding modifier at 15 mL / m in each spawning pond and culture for three days. 2 Add the corresponding modifier and culture for three days.

[0071] Fry cultivation: Three days later, put 1000 fry into each nursery pond and introduce the same water quality for feeding. After stocking, add the corresponding modifier at 15 mL / m in each group. The other cultivation conditions are the same. After 30 days, count the number of harvested tails in each group and calculate the survival rate. The survival rate = number of harvested / number of stocked. The results are shown in Table 1. 2 Add the corresponding modifier, and the other cultivation conditions are the same. After 30 days, count the number of harvested tails in each group and calculate the survival rate. The survival rate = number of harvested / number of stocked. The results are shown in Table 1.

[0072] Table 1 Survival rate of fry in each group

[0073]

[0074] As can be seen from Table 1, the survival rates of the fry in the groups of Examples 1-3 are all above 96%. In Comparative Example 1, since evening primrose, tangerine peel and coltsfoot flower were not fermented with probiotics but directly prepared into an extract, the survival rate of the fry was significantly lower than that of the groups of Examples 1-3.

[0075] Application example weight and total length test

[0076] Measure the body weight and total length of the juvenile red snappers during the breeding period, and evaluate the growth performance of the juvenile red snappers. Randomly select 50 juvenile red snappers in each group, and use a weighing scale with an accuracy of 0.01 g and a ruler with an accuracy of 0.01 mm to measure the body weight and total length of the juvenile red snappers at 30 days and 60 days. The results are shown in Table 2.

[0077] Table 2 Body weight and total length of each group

[0078]

[0079] As can be seen from Table 2, the modifier prepared by the present invention can better improve the body weight and total length of the fish, and the growth rate is faster; and from the data of Examples 1-3, it can be seen that the fry specifications obtained by using the modifier prepared by the present invention are neat, making them more consistent in food grabbing and growth rate. This may be due to reducing the competition and survival pressure caused by individual differences, thereby improving the overall survival rate.

[0080] The above-described embodiments are only descriptions of the preferred embodiments of the present invention, and do not limit the scope of the present invention. Without departing from the design spirit of the present invention, various deformations and improvements made by those of ordinary skill in the art to the technical solutions of the present invention shall fall within the protection scope determined by the claims of the present invention.

Claims

1. A modifier for improving the survival rate of artificial breeding of red nine-spined seabass, characterized in that: By weight, it includes the following components: 15-20 parts of Chinese herbal probiotic fermentation liquid, 8-10 parts of purslane, 8-10 parts of rehmannia root, 5-8 parts of cypress essential oil and 5-8 parts of phenylalanine; The traditional Chinese medicine probiotic fermentation liquid is obtained by fermenting evening primrose, tangerine peel and coltsfoot flower with Lactobacillus reuteri.

2. The improver according to claim 1, characterized in that The weight ratio of evening primrose, tangerine peel and coltsfoot flower is (5-7): (3-5): (2-3); the strain number of Lactobacillus reuteri is CICC 6119.

3. A method for preparing the improver according to claim 1 or 2, characterized in that: The following steps are involved: 5-7 parts of evening primrose, 3-5 parts of tangerine peel and 2-3 parts of coltsfoot flower are mixed and ultrafinely crushed to obtain ultrafine powder, and then soaked and ground into slurry with water; Lactobacillus reuteri liquid is added into the slurry for fermentation, and the supernatant is obtained by centrifugation to obtain a Chinese medicine probiotic fermentation liquid; Purslane and Rehmannia root are dried and crushed into ultrafine powder to obtain a mixture powder; water is added to the mixture powder, and the mixture is boiled and filtered to obtain a decoction A and a medicinal residue; water is added to the medicinal residue, and the mixture is boiled again and filtered to obtain a decoction B; the decoction A and the decoction B are combined to obtain a Chinese medicine extract; The improver is obtained by mixing the fermentation liquid of Chinese medicinal probiotics, the Chinese medicinal extract, cypress essential oil and phenylalanine.

4. The preparation method according to claim 3, characterized in that: The volume ratio of the superfine powder to water is 1:(8-10); The inoculation amount of the Lactobacillus reuteri liquid is 5%, and the concentration of the Lactobacillus reuteri liquid is 4×10 9 cfu / mL.

5. The preparation method according to claim 3, characterized in that: The fermentation conditions are as follows: pH 7, temperature 39° C., and fermentation time 28 hours.

6. The preparation method according to claim 3, characterized in that: The volume ratio of the mixture powder to water is 1:(20-25); the decoction time is 80min-90min.

7. The preparation method according to claim 3, characterized in that: The volume ratio of the medicinal residue to water is 1:(7-9); the time for the second decoction is 50min-70min.

8. Use of the improver as claimed in claim 1 in improving the survival rate of artificial breeding of red nine-spined seabass.

9. The use according to claim 8, characterized in that The dosage of the improver is 10-20 mL / m 2 .

10. Use of the improver according to claim 1 in preparing a product for improving the survival rate of artificial breeding of red nine-spined seabass.

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