Composite liquid preparation for relieving female dysmenorrhea

Abstract

The invention discloses a composite liquid preparation for relieving female dysmenorrhea, and belongs to the technical field of preparations. According to the invention, black sesame, peanut coat, dark plum and dendrobium officinale are selected as main components for relieving dysmenorrhea, and sialic acid and nicotinamide are supplemented to further improve the relieving effect. The composite liquid preparation prepared by the invention can effectively relieve dysmenorrhea, effectively relieve pain and reduce the expression of prostaglandin during dysmenorrhea.

Description

Technical Field

[0001] The present invention relates to a compound liquid preparation for relieving dysmenorrhea in women, and belongs to the technical field of preparations. Background Art

[0002] Dysmenorrhea refers to lower abdominal pain that occurs in women during or before and after menstruation, often accompanied by symptoms such as nausea, vomiting, headache, and low back pain. According to its causes, dysmenorrhea is divided into two categories: primary and secondary. Primary dysmenorrhea is mainly related to excessive uterine contractions, elevated prostaglandin levels, and endometrial ischemia, while secondary dysmenorrhea is mostly caused by diseases such as endometriosis and pelvic inflammation. Dysmenorrhea seriously affects the quality of life of women, especially causing troubles to the work and study of young women.

[0003] Currently, there are a wide variety of drugs or products for relieving dysmenorrhea on the market. Common ones include: Non-steroidal anti-inflammatory drugs (NSAIDs): such as ibuprofen, paracetamol, etc. These drugs relieve pain and inflammation by inhibiting the synthesis of prostaglandins and are commonly used drugs for relieving dysmenorrhea. Hormonal drugs: such as oral contraceptives, mainly relieve dysmenorrhea symptoms by inhibiting ovulation and regulating hormone levels. Traditional Chinese medicines and Chinese patent medicines: Traditional Chinese medicine conditioning relieves dysmenorrhea through the effects of promoting blood circulation to remove blood stasis and warming the meridians to relieve pain. Common traditional Chinese medicine preparations contain ingredients such as motherwort and angelica. Hot compress products: Hot compress patches, warm baby patches, etc. promote local blood circulation through heat therapy, relax uterine muscles, and thus relieve pain. Supplements: such as vitamins B1, B6 and minerals such as magnesium, which help reduce uterine spasms and relieve dysmenorrhea. Non-steroidal anti-inflammatory drugs and hormonal drugs usually have side effects such as gastrointestinal discomfort, ulcers, renal function damage, hormonal level disorders, causing weight gain, breast hyperplasia, skin problems, etc.; while the relief effect of hot compress products is not obvious enough and is limited by individual differences.

[0004] Endless traditional Chinese medicine preparations have emerged. Patent CN105582232A discloses the preparation of a preparation for relieving dysmenorrhea using Corydalis yanhusuo, Chuanxiong, etc.; Patent CN105582518A discloses a traditional Chinese medicine preparation for treating dysmenorrhea; Patent CN117427126A discloses the application of Dendrobium officinale in the preparation of drugs for treating primary dysmenorrhea; Patent CN105663762A discloses the preparation of an oral liquid for relieving dysmenorrhea using Dendrobium officinale, angelica, salvia miltiorrhiza, safflower, etc. In addition, patents for treating dysmenorrhea are uneven in quality, mostly using a combination of multiple blood-activating traditional Chinese medicines for compounding, making it difficult to distinguish their true efficacy. At the same time, the effective components of traditional Chinese medicine prescriptions are complex, and the efficacy is subject to individual differences.

[0005] Currently, there is a rich variety of drugs and products for relieving dysmenorrhea on the market, but each type of product has its limitations. Therefore, developing a liquid preparation with simple raw materials, homologous medicine and food, and rapid effect for relieving dysmenorrhea has great practical value and economic value. Summary of the Invention

[0006] To solve the above problems, the present invention selects black sesame seeds, peanut skins, smoked plums, and Dendrobium officinale as the main ingredients for relieving dysmenorrhea, and is supplemented with sialic acid and niacinamide to further improve the relief effect.

[0007] The first object of the present invention is to provide a compound liquid preparation for relieving dysmenorrhea in women. In the compound liquid preparation, by mass fraction, it contains: 4 - 8 parts of black sesame seed extract, 4 - 8 parts of peanut skin extract, 2 - 4 parts of Dendrobium officinale extract, 2 - 4 parts of smoked plum extract, 0.5 - 2 parts of sialic acid, and 0.5 - 2 parts of niacinamide, and the balance is made up to 100 parts with water.

[0008] In one embodiment, the preparation method of the black sesame seed extract is as follows: dry and crush the black sesame seeds to obtain black sesame powder; mix the black sesame powder with ethanol, extract, and dry to prepare it.

[0009] The preparation method of the peanut skin extract is as follows: crush the peanut skins to obtain peanut skin powder; mix the peanut skin powder with ethanol, extract, and dry to prepare it.

[0010] The preparation method of the Dendrobium officinale extract is as follows: crush the Dendrobium officinale to obtain Dendrobium officinale powder; mix the Dendrobium officinale powder with water, extract, and dry to prepare it.

[0011] The preparation method of the smoked plum extract is as follows: mix the smoked plums with water, extract, and dry to prepare it.

[0012] In one embodiment, the dosage ratio of the black sesame powder to ethanol is 1 g: 5 - 15 mL.

[0013] The dosage ratio of the peanut skin powder to ethanol is 1 g: 5 - 20 mL.

[0014] The dosage ratio of the Dendrobium officinale powder to water is 1 g: 10 - 30 mL.

[0015] The dosage ratio of the smoked plums to water is 1 g: 10 - 30 mL.

[0016] In one embodiment, the extraction in mixing the black sesame powder with ethanol for extraction is carried out at an ultrasonic power of 50 - 150 W and a temperature of 40 - 50 °C for 2 - 4 h.

[0017] In one embodiment, the extraction in mixing the peanut skin powder with ethanol for extraction is carried out at an ultrasonic power of 50 - 150 W and a temperature of 40 - 50 °C for 1 - 3 h.

[0018] In one embodiment, the extraction in mixing the Dendrobium officinale powder with water for extraction is carried out at an ultrasonic power of 50 - 150 W and a temperature of 70 - 85 °C for 1 - 3 h.

[0019] In one embodiment, the extraction in mixing smoked plum with water for extraction is carried out at an ultrasonic power of 50 - 150 W and a temperature of 40 - 50 °C for 2 - 4 h.

[0020] The second object of the present invention is to provide a product containing any of the above-mentioned compound liquid preparations;

[0021] Optionally, the product includes food, medicine or health care products.

[0022] The third object of the present invention is to provide the use of black sesame extract or peanut skin extract in the preparation of a product for relieving dysmenorrhea. The preparation method of the black sesame extract is as follows: drying and crushing black sesame to obtain black sesame powder; mixing the black sesame powder with ethanol, extracting and drying to prepare it;

[0023] The preparation method of the peanut skin extract is as follows: crushing peanut skin to obtain peanut skin powder; mixing the peanut skin powder with ethanol, extracting and drying to prepare it;

[0024] Optionally, the preparation method of the black sesame extract is as follows: mixing the black sesame powder with ethanol at a dosage ratio of 1 g : 5 - 15 mL, extracting at an ultrasonic power of 50 - 150 W and a temperature of 40 - 50 °C for 2 - 4 h;

[0025] Optionally, the preparation method of the peanut skin extract is as follows: mixing the peanut skin powder with ethanol at a dosage ratio of 1 g : 5 - 20 mL, extracting at an ultrasonic power of 50 - 150 W and a temperature of 40 - 50 °C for 1 - 3 h.

[0026] The fourth object of the present invention is to provide a method for improving the biological activity of dendrobium officinale, sialic acid or niacinamide. The method is to mix dendrobium officinale, niacinamide, sialic acid with black sesame extract, peanut skin extract and smoked plum extract to obtain a mixture with improved activity;

[0027] Optionally, the biological activity is the effect of relieving dysmenorrhea;

[0028] Optionally, in the mixture with improved activity, by mass, the black sesame extract is 4 - 8 parts, the peanut skin extract is 4 - 8 parts, the dendrobium officinale extract is 2 - 4 parts, the smoked plum extract is 2 - 4 parts, sialic acid is 0.5 - 2 parts, and niacinamide is 0.5 - 2 parts.

[0029] In one embodiment, the preparation method of the black sesame extract is as follows: drying and crushing black sesame to obtain black sesame powder; mixing the black sesame powder with ethanol, extracting and drying to prepare it;

[0030] The preparation method of the peanut skin extract is as follows: crushing peanut skin to obtain peanut skin powder; mixing the peanut skin powder with ethanol, extracting and drying to prepare it;

[0031] The preparation method of the Dendrobium officinale extract is as follows: Dendrobium officinale is crushed to obtain Dendrobium officinale powder; the Dendrobium officinale powder and water are mixed, extracted, and dried to obtain it.

[0032] The preparation method of the Prunus mume extract is as follows: Prunus mume and water are mixed, extracted, and dried to obtain it.

[0033] In one embodiment, the dosage ratio of black sesame powder to ethanol is 1 g: 5 - 15 mL;

[0034] The dosage ratio of peanut skin powder to ethanol is 1 g: 5 - 20 mL;

[0035] The dosage ratio of Dendrobium officinale powder to water is 1 g: 10 - 30 mL;

[0036] The dosage ratio of Prunus mume to water is 1 g: 10 - 30 mL.

[0037] In one embodiment, the extraction in the mixing and extraction of black sesame powder and ethanol is carried out at an ultrasonic power of 50 - 150 W and a temperature of 40 - 50 °C for 2 - 4 h.

[0038] In one embodiment, the extraction in the mixing and extraction of peanut skin powder and ethanol is carried out at an ultrasonic power of 50 - 150 W and a temperature of 40 - 50 °C for 1 - 3 h.

[0039] In one embodiment, the extraction in the mixing and extraction of Dendrobium officinale powder and water is carried out at an ultrasonic power of 50 - 150 W and a temperature of 70 - 85 °C for 1 - 3 h.

[0040] In one embodiment, the extraction in the mixing and extraction of Prunus mume and water is carried out at an ultrasonic power of 50 - 150 W and a temperature of 40 - 50 °C for 2 - 4 h.

[0041] Advantages of the present invention

[0042] The present invention selects black sesame, peanut skin, Prunus mume, and Dendrobium officinale as the main components for relieving dysmenorrhea, and at the same time supplements with sialic acid and niacinamide to further improve the relief effect. The compound liquid preparation prepared by the present invention can effectively relieve dysmenorrhea, effectively relieve pain, and reduce the expression of prostaglandins during dysmenorrhea. Specific embodiments

[0043] The following are descriptions of the preferred embodiments of the present invention. It should be understood that the embodiments are for better explaining the present invention and are not used to limit the present invention.

[0044] Dendrobium officinale, peanut skin, black sesame, and Prunus mume are all commercially available;

[0045] Niacinamide is purchased from a powerful supplier of Shanghai Canal Materials Technology;

[0046] N-acetylneuraminic acid was purchased from Changzhou 123 Biotechnology Co., Ltd.;

[0047] Diethylstilbestrol injection was purchased from Tianjin KingYork Pharmaceutical Co., Ltd.;

[0048] Oxytocin injection was purchased from Shanghai Hefeng Pharmaceutical Co., Ltd.;

[0049] Rat prostaglandin PGF2ɑ enzyme-linked immunosorbent assay kit and rat prostaglandin PGF2ɑ enzyme-linked immunosorbent assay kit were purchased from Shanghai BlueGene Biotechnology Co., Ltd.

[0050] 2. Test method:

[0051] Clean-grade female SD rats, weighing 200±15 g, were provided by the Experimental Animal Center of Shanghai University of Traditional Chinese Medicine. Before the experiment, the animals were acclimatized to the environment indoors for 1 week at a room temperature of 20-25 °C.

[0052] (1) Observation of pain writhing response in rats

[0053] Record the number of writhing animals, the number of writhing times, and the average number of writhing times in each group within 30 min after intraperitoneal injection of oxytocin in rats. The writhing response was defined as the concave abdomen of the rat, the extension of the trunk and hind limbs, and the distortion of the buttocks.

[0054] (2) Detection of PGF2ɑ and PGE2 contents in rat uterine tissue

[0055] Thirty minutes after the observation of the writhing response, the rats were sacrificed, the uterus was dissected, 0.5 g was precisely weighed, 1 mL of normal saline was added, homogenized, centrifuged, and the supernatant was taken. The contents of PGF2ɑ and PGE2 in the rat uterus were detected using the prostaglandin PGF2ɑ enzyme-linked immunosorbent assay kit and the prostaglandin PGE2 enzyme-linked immunosorbent assay kit.

[0056] (3) Determination of the contraction amplitude and frequency of uterine smooth muscle

[0057] After dissecting the rat uterus, quickly dissect one side of the rat uterus, cut a 2-cm section of the uterus, place it in a small trough containing 30 mL of Locke's solution, connect it to a sensor, maintain the water bath at (36±0.5) °C, and continuously supply oxygen. Use the MS-302 multimedia biological signal recording and analysis system to record the contraction curve of uterine smooth muscle and collect the data of contraction amplitude and frequency.

[0058] Locke's solution (1000 mL): NaCl 9.0 g, KCl 0.42 g, CaCl 2 0.24 g, NaHCO 3 0.1-0.3 g, glucose 1.0-2.5 g.

[0059] Example 1: Preparation of the preparation

[0060] 1. Preparation of black sesame extract

[0061] (1) Dry and crush black sesame seeds to obtain black sesame powder; mix the black sesame powder and 85% ethanol at a dosage ratio of 1 g: 5 mL, and extract at an ultrasonic power of 100 W, 45 °C, and 100 rpm for 3 h;

[0062] (2) After the extraction is completed, filter to obtain the supernatant, and freeze-dry to obtain the black sesame extract.

[0063] 2. Preparation of peanut skin extract

[0064] (1) Crush peanut skins to obtain peanut skin powder; mix the peanut skin powder and 65% ethanol at a dosage ratio of 1 g: 10 mL, and extract at an ultrasonic power of 100 W, 45 °C, and 100 rpm for 1 h;

[0065] (2) After the extraction is completed, filter to obtain the supernatant, and freeze-dry to obtain the peanut skin extract.

[0066] 3. Preparation of Dendrobium officinale extract

[0067] (1) Crush Dendrobium officinale to obtain Dendrobium officinale powder; mix the Dendrobium officinale powder and water at a dosage ratio of 1 g: 20 mL, and extract at an ultrasonic power of 100 W, 80 °C, and 100 rpm for 2 h;

[0068] (2) After the extraction is completed, filter to obtain the supernatant, and freeze-dry to obtain the Dendrobium officinale extract.

[0069] 4. Preparation of smoked plum extract

[0070] (1) Mix smoked plums and water at a dosage ratio of 1 g: 20 mL, and extract at an ultrasonic power of 100 W, 45 °C, and 100 rpm for 3 h;

[0071] (2) After the extraction is completed, filter to obtain the supernatant, and freeze-dry to obtain the smoked plum extract.

[0072] 5. Preparation of compound liquid preparation

[0073] According to the mass parts, mix 6 parts of black sesame extract, 6 parts of peanut skin extract, 3 parts of Dendrobium officinale extract, 3 parts of smoked plum extract, 1 part of sialic acid, and 1 part of niacinamide, and make up to 100 parts with water to prepare a compound liquid preparation (that is, 100 g of the liquid preparation contains 20 g of the active ingredient).

[0074] Control Example 1: Without adding peanut skin extract

[0075] On the basis of Example 1, the formulation ratio of the compound liquid preparation in Step 5 was changed as follows: By mass parts, 6 parts of black sesame extract, 6 parts of smoked plum extract, 6 parts of Dendrobium officinale extract, 1 part of sialic acid, and 1 part of niacinamide were mixed, and water was added to make up 100 parts to prepare the compound liquid preparation.

[0076] Comparative Example 2: Without adding sialic acid and niacinamide

[0077] On the basis of Example 1, the formulation ratio of the compound liquid preparation in Step 5 was changed as follows: By mass parts, 6 parts of black sesame extract, 6 parts of peanut skin extract, 4 parts of Dendrobium officinale extract, and 4 parts of smoked plum extract were mixed, and water was added to make up 100 parts to prepare the compound liquid preparation.

[0078] Comparative Example 3: Without adding black sesame extract

[0079] On the basis of Example 1, the formulation ratio of the compound liquid preparation in Step 5 was changed as follows: By mass parts, 6 parts of Dendrobium officinale extract, 6 parts of peanut skin extract, 6 parts of smoked plum extract, 1 part of sialic acid, and 1 part of niacinamide were mixed, and water was added to make up 100 parts to prepare the compound liquid preparation.

[0080] Example 2: Evaluation of the preparation function

[0081] 1. Grouping of animal experiments

[0082] Thirty SD rats were randomly divided into a blank group (5 rats), a model group (5 rats), an Example 1 group (5 rats), a Comparative Example 1 group (5 rats), a Comparative Example 2 group (5 rats), and a Comparative Example 3 group (5 rats).

[0083] Blank group: From the tenth day, gavaged with an equal volume of distilled water;

[0084] Model group: Subcutaneously injected with diethylstilbestrol for 10 consecutive days. Among them, on the first day and the tenth day, 0.5 mg / rat was injected, and from the second to the ninth day, 0.25 mg / rat was injected; From the tenth day, gavaged with an equal volume of distilled water for two consecutive days; 40 minutes after the last gavage, the rats were intraperitoneally injected with 2 U of oxytocin.

[0085] Example 1 group: Subcutaneously injected with diethylstilbestrol for 10 consecutive days. Among them, on the first day and the tenth day, 0.5 mg / rat was injected, and from the second to the ninth day, 0.25 mg / rat was injected; From the tenth day, gavaged with the compound liquid preparation prepared in Example 1 for two consecutive days, and the dose was 3 g of active ingredient / (kg·d); 40 minutes after the last administration, each group of rats was intraperitoneally injected with 2 U of oxytocin.

[0086] Control group 1: Diethylstilbestrol was subcutaneously injected continuously for 10 days. Among them, 0.5 mg / rat was injected on the first and tenth days, and 0.25 mg / rat was injected from the second to the ninth day. Starting from the tenth day, the compound liquid preparation obtained in Control group 1 was administered by gavage for two consecutive days at a dose of 3 g of active ingredient / (kg·d). 40 minutes after the last administration, 2 U of oxytocin was intraperitoneally injected into the rats in each group.

[0087] Control group 2: Diethylstilbestrol was subcutaneously injected continuously for 10 days. Among them, 0.5 mg / rat was injected on the first and tenth days, and 0.25 mg / rat was injected from the second to the ninth day. Starting from the tenth day, the compound liquid preparation obtained in Control group 2 was administered by gavage for two consecutive days at a dose of 3 g of active ingredient / (kg·d). 40 minutes after the last administration, 2 U of oxytocin was intraperitoneally injected into the rats in each group.

[0088] Control group 3: Diethylstilbestrol was subcutaneously injected continuously for 10 days. Among them, 0.5 mg / rat was injected on the first and tenth days, and 0.25 mg / rat was injected from the second to the ninth day. Starting from the tenth day, the compound liquid preparation obtained in Control group 3 was administered by gavage for two consecutive days at a dose of 3 g of active ingredient / (kg·d). 40 minutes after the last administration, 2 U of oxytocin was intraperitoneally injected into the rats in each group.

[0089] 2. Test methods

[0090] (1) Writhing times of SD rats

[0091] The number of writhing animals, the number of writhing times and the average number of writhing times in each group were recorded within 30 minutes after the intraperitoneal injection of oxytocin in rats. The writhing reaction was judged by the criteria that the rat's abdomen was concave, the trunk and hind limbs were extended, and the hip was twisted.

[0092] Table 1 Comparison of writhing times of SD rats

[0093]

[0094]

[0095] As shown in Table 1, compared with the model group, the writhing times of rats in the blank group were significantly increased; although the compound liquid preparations prepared in Example 1 and Control groups 1-3 could all reduce the writhing times, the effect of the compound liquid preparation in Example 1 was significantly better than that in Control groups 1-3.

[0096] (2) Detection of PGF2ɑ and PGE2 contents in rat uterine tissue

[0097] Thirty minutes after the observation of the writhing response ended, the rats were sacrificed, the uterine tissues were dissected, 0.5 g was weighed, 1 mL of normal saline was added, homogenized, centrifuged, and the supernatant was taken. The contents of prostaglandin PGF2α and prostaglandin PGE2 in the uterine tissues of the rats were detected using the prostaglandin PGF2α enzyme-linked immunosorbent assay kit and the prostaglandin PGE2 enzyme-linked immunosorbent assay kit.

[0098] Table 2 Comparison of the contents of PGF2α and PGE2 in the uterine tissues of SD rats in each group

[0099] Group PGF2ɑ (pg / mL) PGE2 (ng / mL) Blank group 54.02±7.12 3.55±0.74 Model group 193.65±19.84 1.69±1.07 Example 1 group 103.11±9.52 4.37±1.53 Comparative example 1 group 148.61±11.25 2.57±0.63 Comparative example 2 group 139.97±13.21 2.76±1.24 Comparative example 3 group 130.37±6.57 2.98±1.58

[0100] PGF2α is a strong vasoconstrictor and also has the effect of promoting uterine contractions. During the inflammatory process, the release of PGF2α is usually associated with tissue damage, cell injury, and the production of other inflammatory mediators; PGE2 usually has various physiological functions such as regulating immune responses, anti-inflammatory, and anticoagulant effects during inflammation. In inflammatory conditions, the production of PGE2 is usually related to inhibiting the inflammatory response and reducing tissue damage. As shown in Table 2, compared with the model group, the content of PGF2α in the uterine tissues of the rats in Example 1 and Comparative Examples 1-3 decreased while the content of PGE2 increased. Among them, the lowest PGF2α and the highest PGE2 contents were in Example 1, which was significantly better than that in Comparative Examples 1-3.

[0101] (3) Determination of the contraction amplitude and frequency of uterine smooth muscle

[0102] After dissecting the uterine tissues of the rats, one side of the uterus of the rats was quickly dissected, 2 cm of the uterus was cut, placed in a small trough containing 30 mL of Locke's solution, connected to a sensor, the water bath was maintained at (36 ± 0.5) °C, and oxygen was continuously introduced. The contraction curve of uterine smooth muscle was recorded using the MS-302 multimedia biological signal recording and analysis system, and the data of contraction amplitude and frequency were collected.

[0103] Table 3 Comparison of the contraction amplitude and frequency of uterine smooth muscle of rats in each group

[0104]

[0105]

[0106] As shown in Table 3, compared with the model group, the contraction amplitude and frequency of uterine smooth muscle of the rats in Example 1 and Comparative Examples 1-3 decreased significantly. Among them, the effect of Example 1 group was the best, which was significantly better than that of Comparative Examples 1-3.

[0107] Based on the above results, it can be seen from the comparison between Example 1 and Comparative Examples 1-3 that in the compound liquid preparation, peanut tegument extract, Dendrobium officinale extract, sialic acid, and niacinamide have a synergistic effect. Although the compound liquid preparation prepared by lacking any one of these components also has the effect of relieving dysmenorrhea, the effect is significantly lower than that of the compound liquid preparation prepared by adding all four components.

[0108] Although the present invention has been disclosed above with preferred embodiments, it is not intended to limit the present invention. Anyone familiar with this technology can make various modifications and alterations without departing from the spirit and scope of the present invention. Therefore, the protection scope of the present invention should be defined by the claims.

Claims

1. A composite liquid preparation for relieving dysmenorrhea in women, characterized in that: The composite liquid preparation contains, by weight, 4 to 8 parts of black sesame extract, 4 to 8 parts of peanut skin extract, 2 to 4 parts of dendrobium officinale extract, 2 to 4 parts of black plum extract, 0.5 to 2 parts of bird's nest acid, and 0.5 to 2 parts of niacinamide, which is made up to 100 parts with water.

2. The composite liquid preparation according to claim 1, characterized in that: The preparation method of the black sesame extract comprises: drying and crushing the black sesame to obtain black sesame powder; mixing the black sesame powder and ethanol, extracting, and drying to obtain the black sesame extract; The preparation method of the peanut skin extract comprises: crushing the peanut skin to obtain peanut skin powder; mixing the peanut skin powder with ethanol, extracting, and drying to obtain the peanut skin extract; The preparation method of the Dendrobium officinale extract comprises: crushing the Dendrobium officinale to obtain the Dendrobium officinale powder; mixing the Dendrobium officinale powder with water, extracting, and drying to obtain the extract; The preparation method of the black plum extract is: mixing black plum and water, extracting, and drying to prepare the extract.

3. The composite liquid preparation according to claim 2, characterized in that: The dosage ratio of black sesame powder to ethanol is 1g:5-15mL; The dosage ratio of peanut skin powder to ethanol is 1g:5-20mL; The dosage ratio of Dendrobium officinale powder and water is 1g:10-30mL; The dosage ratio of black plum and water is 1g:10~30mL.

4. The composite liquid preparation according to claim 2, characterized in that: The black sesame powder and ethanol are mixed and extracted at an ultrasonic power of 50 to 150 W and 40 to 50° C. for 2 to 4 hours.

5. The composite liquid preparation according to claim 2, characterized in that: The peanut skin powder and ethanol are mixed and extracted at an ultrasonic power of 50 to 150 W and 40 to 50° C. for 1 to 3 hours.

6. The composite liquid preparation according to claim 2, characterized in that: The Dendrobium officinale powder and water are mixed, and the extraction is performed at an ultrasonic power of 50-150W and 70-85°C for 1-3h.

7. The composite liquid preparation according to claim 2, characterized in that: Mix the black plum and water, and extract at an ultrasonic power of 50-150W and 40-50°C for 2-4h.

8. A product, characterized in that The product contains the composite liquid preparation according to any one of claims 1 to 8; Optionally, the product includes food, medicine or health care product.

9. Use of black sesame extract or peanut skin extract in preparing a product for relieving dysmenorrhea, characterized in that: The preparation method of the black sesame extract comprises: drying and crushing the black sesame to obtain black sesame powder; mixing the black sesame powder and ethanol, extracting, and drying to obtain the black sesame extract; The preparation method of the peanut skin extract comprises: crushing the peanut skin to obtain peanut skin powder; mixing the peanut skin powder with ethanol, extracting, and drying to obtain the peanut skin extract; Optionally, the preparation method of the black sesame extract is: mixing black sesame powder and ethanol in a dosage ratio of 1 g: 5-15 mL, and extracting at an ultrasonic power of 50-150 W and 40-50° C. for 2-4 hours; Optionally, the peanut skin extract is prepared by mixing peanut skin powder and ethanol in a dosage ratio of 1 g: 5-20 mL, and extracting at an ultrasonic power of 50-150 W and 40-50° C. for 1-3 hours.

10. A method for improving the biological activity of Dendrobium officinale, bird's nest acid or nicotinamide, characterized in that: Mixing Dendrobium officinale, niacinamide, bird's nest acid, black sesame extract, peanut skin extract, and black plum extract to obtain a mixture with enhanced activity; Optionally, the biological activity is the effect of relieving dysmenorrhea; Optionally, in the mixture with enhanced activity, the following are included by weight: 4-8 parts of black sesame extract, 4-8 parts of peanut skin extract, 2-4 parts of Dendrobium officinale extract, 2-4 parts of black plum extract, 0.5-2 parts of bird's nest acid, and 0.5-2 parts of niacinamide.

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