Extraction method of soluble protein extract of fresh tobacco leaves

Abstract

The invention relates to the technical field of protein extraction, in particular to a method for extracting soluble protein extract of fresh tobacco leaves, which comprises the following steps: putting decolorized and degreased fresh tobacco leaf filter residues into a flash extractor containing an extraction monitoring system for extraction and filtration; adding neutral salt into the supernatant until the salt solution is in a saturated state, and drying the precipitate to obtain the soluble protein extract of the fresh tobacco leaves; wherein the soluble protein extraction progress is obtained through calculation, and the extraction state is evaluated; obtaining related factor data under the monitoring period corresponding to the extracted unqualified signals, performing influence analysis on the related factor data and the soluble protein extraction progress to obtain influence length and influence depth, and determining factors influencing the soluble protein extraction progress by comprehensively analyzing the influence length and the influence depth; regulating and controlling factors according to the factors influencing the extraction progress of the soluble protein; factors are regulated and controlled in time, and the extraction efficiency of the soluble protein of the fresh tobacco leaves is guaranteed.

Description

Technical Field

[0001] The present invention relates to the technical field of protein extraction, and specifically relates to a method for extracting soluble protein extract from fresh tobacco leaves. Background Art

[0002] Proteins are an important material basis for human activities. In the global supply of protein resources, most are plant proteins, accounting for 70% of the total protein, while animal proteins only account for 30%. In addition, plant proteins with advantages such as economy, nutrition, and functionality are playing an increasingly important role in establishing a healthy diet structure and are attracting more and more attention.

[0003] Leaf protein is a protein product separated from the juice after fresh plant green leaves are chopped and pressed. Large-scale development and utilization of leaf protein resources is an effective way to increase the total protein production. Among the many components of tobacco leaves, the protein content is relatively rich. For example, the protein content in burley tobacco is as high as 20.48%. Animal experiments on tobacco leaf protein show that its nutritional value is higher than that of soybean protein and casein.

[0004] In the prior art, during the extraction process of the soluble protein extract from fresh tobacco leaves, due to changes in extraction parameters, the extraction process deviates from the expected process, making it difficult to complete the start-stop and regulation strategies for the extraction of soluble protein from fresh tobacco leaves in a timely manner, reducing human operation deviations, and improving the problem of production consistency. Summary of the Invention

[0005] The purpose of the present invention is to provide a method for extracting soluble protein extract from fresh tobacco leaves. The technical problem solved by the present invention is: due to changes in extraction parameters, the extraction process deviates from the expected process, making it difficult to complete the start-stop and regulation strategies for the extraction of soluble protein from fresh tobacco leaves in a timely manner, reducing human operation deviations, and improving the problem of production consistency.

[0006] The purpose of the present invention can be achieved through the following technical solutions: A method for extracting soluble protein extract from fresh tobacco leaves, comprising the following steps: Placing the decolorized and defatted fresh tobacco leaf residue in a flash extractor containing an extraction monitoring system for extraction and filtration; Adding neutral salt to the supernatant until the salt solution is saturated, precipitating and drying to obtain the soluble protein extract of fresh tobacco leaves; Among them, the steps of extraction monitoring performed by the flash extractor are as follows: Obtaining the soluble protein content during the extraction process of the fresh tobacco leaf residue solution in the flash extractor, calculating the soluble protein extraction progress, and evaluating the extraction status; If the extraction status is an unqualified extraction signal, a detection instruction is generated; When the detection instruction is obtained, relevant factor data for the monitoring period corresponding to the unqualified extraction signal is acquired, and an impact analysis is performed with the soluble protein extraction progress to obtain the impact length and impact depth. Through comprehensive analysis of the impact length and impact depth, the factors affecting the soluble protein extraction progress are determined; Based on the factors affecting the soluble protein extraction progress, the factors are regulated.

[0007] As a further solution of the present invention: The calculation process of the soluble protein extraction progress is as follows: A monitoring period is set. During the extraction process of the fresh tobacco leaf filter residue solution in the flash extractor, the soluble protein content for different monitoring periods is obtained to obtain the soluble protein extraction progress.

[0008] As a further solution of the present invention: The process of evaluating the extraction status is as follows: If the soluble protein extraction progress is less than the standard soluble protein content for different monitoring periods, an unqualified extraction signal is generated.

[0009] As a further solution of the present invention: The calculation process of the impact length is as follows: Relevant factor data for the monitoring period corresponding to the unqualified extraction signal is acquired, and a relevant factor curve is obtained by fitting; The soluble protein extraction progress for the monitoring period corresponding to the unqualified extraction signal is acquired, and an extraction progress curve is obtained by fitting; The monitoring period is divided into several monitoring time segments, and an abnormal similarity analysis is performed between the relevant factor curve and the extraction progress curve to obtain an abnormal mutual influence curve segment; Calculate the proportion of the total time of the abnormal mutual influence curve segment in the duration of the monitoring period to obtain the impact length.

[0010] As a further solution of the present invention: The calculation process of the impact depth is as follows: The relevant factor slope and extraction progress slope of the abnormal mutual influence curve segment are acquired, and the deviation mean value of the relevant factor slope and the extraction progress slope is calculated to obtain the impact depth.

[0011] As a further solution of the present invention: The determination process of the factors affecting the soluble protein extraction progress is as follows: Calculate the ratio of the impact depth to the impact length to obtain an impact degree value; If the impact depth is less than the impact depth threshold, an impact signal is generated.

[0012] As a further solution of the present invention: The process of the abnormal similarity analysis includes: Obtain the mean value of the relevant factor data for the relevant factor curve at any number of monitoring time periods. If the mean value of the relevant factor data is lower than the process standard value, then mark the curve corresponding to this monitoring time period as an abnormal curve segment of the relevant factor. Obtain the extraction progress of the relevant factor curve at any number of monitoring time periods. If the extraction progress is lower than the extraction progress standard value, then mark the curve corresponding to this monitoring time period as an abnormal curve segment of the extraction progress.

[0013] As a further solution of the present invention: The process of abnormal similarity analysis further includes: Obtain the abnormal curve segment of the relevant factor and the normal curve segment of the extraction progress at the same monitoring time period, calculate the Pearson correlation coefficient of the corresponding data of the abnormal curve segment of the relevant factor and the normal curve segment of the extraction progress at the same moment. If the Pearson correlation coefficient is greater than the coefficient threshold, mark the abnormal curve segment of the relevant factor and the normal curve segment of the extraction progress at the same monitoring time period as an abnormal curve segment of mutual influence.

[0014] As a further solution of the present invention: The process of regulating the factor includes: Obtain the extraction progress of the soluble protein at the monitoring period corresponding to the extraction unqualified signal, and determine the compensation ratio. Multiply the compensation ratio by the mean value of the relevant factor data at the monitoring period corresponding to the extraction unqualified signal to obtain the factor regulation value for the subsequent monitoring period.

[0015] As a further solution of the present invention: The process of determining the compensation ratio is: Calculate the deviation ratio of the extraction progress of the soluble protein at the monitoring period corresponding to the extraction unqualified signal to the extraction progress standard value.

[0016] Advantages of the present invention: The present invention monitors the soluble protein content in real time during the extraction process, judges the extraction efficiency or progress of the soluble protein extract of fresh tobacco leaves, and according to the judgment result, controls the extraction process in a timely and effective manner, optimizes the start-stop strategy. It can also identify the influencing factors of the extraction process according to the judgment result, and timely regulate the factors under the determined influencing factors to ensure the extraction efficiency of the soluble protein of fresh tobacco leaves. Description of the Drawings

[0017] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following will briefly introduce the drawings required for the description of the embodiments. Obviously, the following drawings are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained according to these drawings.

[0018] Figure 1It is a flowchart of a method for extracting soluble protein extract from fresh tobacco leaves provided in Embodiment 1 of the present invention; Figure 2 It is a flowchart of extraction monitoring in a method for extracting soluble protein extract from fresh tobacco leaves provided in Embodiment 2 of the present invention. Detailed implementation manners

[0019] In order to enable those skilled in the art to better understand the solution of the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0020] Embodiment 1

[0021] As Figure 1 and Figure 2 shown, a method for extracting soluble protein extract from fresh tobacco leaves provided in Embodiment 1 of the present invention includes the following steps: Add the decolorized and defatted fresh tobacco leaf residue to a NaCl solution with a concentration of 0.01 mol / L, then adjust the pH value to 7.0 - 8.0 with a NaOH solution with a concentration of 0.1 mol / L. After soaking for a period of time, place it in a flash extractor containing an extraction monitoring system for extraction and filtration. After centrifuging the filtrate to remove the precipitate, take the supernatant for standby; wherein, the model of the flash extractor: KM1-JHBE-50T; Add neutral salt to the supernatant until the salt solution is saturated. Let it stand to fully precipitate the protein, then centrifuge. After the precipitate is desalted by dialysis, place it in a vacuum drying oven for low-temperature drying to obtain the soluble protein extract; By using a flash extractor containing an extraction monitoring system to optimize the start-stop and control strategies for extracting soluble protein from fresh tobacco leaves, it is possible to effectively reduce human operation errors and improve production consistency; The steps of extraction monitoring performed by the flash extractor are as follows: S1. Obtain the soluble protein content in the process of extracting the fresh tobacco leaf residue solution in the flash extractor, calculate the soluble protein extraction progress, and evaluate the extraction status; Specifically, the calculation process of the soluble protein extraction progress is: Set the monitoring period. During the extraction process of the fresh tobacco leaf residue solution in the flash extractor, obtain the soluble protein content in different monitoring periods to obtain the soluble protein extraction progress; It should be explained that the determination of soluble proteins is based on the color reaction of proteins with specific reagents. The absorbance value is measured by spectrophotometry and the concentration is calculated by comparing with the standard curve. Compare the extraction progress of soluble proteins with the standard soluble protein content in different monitoring periods. If the extraction progress of soluble proteins is less than the standard soluble protein content in different monitoring periods, an unqualified extraction signal is generated. If the extraction progress of soluble proteins is greater than or equal to the standard soluble protein content in different monitoring periods, a qualified extraction signal is generated. S2. Generate extraction instructions according to the evaluated extraction status. Specifically, the process of generating extraction instructions is as follows: When a qualified extraction signal is obtained, compare the extraction progress of soluble proteins with the standard soluble protein content in the final monitoring period. If it reaches, generate an extraction stop instruction; if not, generate an extraction continue instruction. It should be explained that when an extraction stop instruction is obtained, it means that the extraction work of soluble proteins from the current fresh tobacco leaves is completed and the current extraction work can be terminated. When an extraction continue instruction is obtained, it means that the extraction work of soluble proteins from the current fresh tobacco leaves is not completed and the extraction work needs to be continued. When an unqualified extraction signal is obtained, generate a detection instruction. S3. When a detection instruction is obtained, obtain the relevant factor data in the monitoring period corresponding to the unqualified extraction signal, conduct an impact analysis with the extraction progress of soluble proteins, obtain the impact length and impact depth, and determine the factors affecting the extraction progress of soluble proteins through comprehensive analysis of the impact length and impact depth. Specifically, the calculation process of the impact length and impact depth is as follows: Obtain the relevant factor data in the monitoring period corresponding to the unqualified extraction signal and fit to obtain the relevant factor curve; among them, the relevant factor data includes extraction speed and extraction voltage. In addition, obtain the extraction progress of soluble proteins in the monitoring period corresponding to the unqualified extraction signal and fit to obtain the extraction progress curve. Divide the monitoring period into several monitoring time periods, conduct an abnormal similarity analysis between the relevant factor curve and the extraction progress curve to obtain the mutually influential abnormal curve segments. Calculate the proportion of the total time of the mutually influential abnormal curve segments in the duration of the monitoring period to obtain the impact length. Obtain the relevant factor slope and extraction progress slope of the mutually influential abnormal curve segments, calculate the deviation mean of the relevant factor slope and the extraction progress slope to obtain the impact depth. Calculate the ratio of the impact depth to the impact length to obtain the impact degree value. Compare the influence depth with the influence depth threshold; If the influence depth is greater than or equal to the influence depth threshold, a non-influence signal is generated; If the influence depth is less than the influence depth threshold, an influence signal is generated; It should be noted that: when an influence signal is generated, it means that this factor has a high impact on the progress of soluble protein extraction; when a non-influence signal is generated, it means that this factor has no high impact on the progress of soluble protein extraction; Among them, the process of abnormal similarity analysis is as follows: First, perform abnormal analysis: Obtain the mean value of the relevant factor data of the relevant factor curve at any number of monitoring time periods. If the mean value of the relevant factor data is lower than the process standard value, mark the curve corresponding to this monitoring time period as the abnormal curve segment of the relevant factor; otherwise, mark the curve corresponding to this monitoring time period as the normal curve segment of the relevant factor; Obtain the extraction progress of the relevant factor curve at any number of monitoring time periods. If the extraction progress is lower than the extraction progress standard value, mark the curve corresponding to this monitoring time period as the abnormal curve segment of the extraction progress; otherwise, mark the curve corresponding to this monitoring time period as the normal curve segment of the extraction progress; First, perform similarity analysis: Obtain the abnormal curve segment of the relevant factor and the normal curve segment of the extraction progress at the same monitoring time period, and calculate the Pearson correlation coefficient of the corresponding data of the abnormal curve segment of the relevant factor and the normal curve segment of the extraction progress at the same moment. If the Pearson correlation coefficient is greater than 0.85, mark the abnormal curve segment of the relevant factor and the normal curve segment of the extraction progress at the same monitoring time period as the mutually influencing abnormal curve segment; Exemplarily, obtain the extraction voltage data under the monitoring period corresponding to the extraction unqualified signal, and fit to obtain the extraction voltage curve; And obtain the soluble protein extraction progress under the monitoring period corresponding to the extraction unqualified signal, and fit to obtain the extraction progress curve; Divide the monitoring period into several monitoring time periods, perform abnormal similarity analysis on the extraction voltage curve and the extraction progress curve, and obtain the mutually influencing abnormal curve segment; Calculate the proportion of the total time of the mutually influencing abnormal curve segment in the duration of the monitoring period to obtain the influence length; Obtain the extraction voltage slope and the extraction progress slope of the mutually influencing abnormal curve segment, and calculate the mean deviation of the extraction voltage slope and the extraction progress slope to obtain the influence depth; Comprehensively analyze the influence length and the influence depth to obtain the influence degree value; among them, the comprehensive analysis can adopt the method of weight calculation. The weight coefficient of the influence length is 0.63, and the weight coefficient of the influence depth is 0.37; Among them, the process of abnormal similarity analysis is as follows: First, perform anomaly analysis: Obtain the average value of the extraction voltage data of the extraction voltage curve in any number of monitoring periods. If the average value of the extraction voltage data is lower than the process standard value, mark the curve corresponding to this monitoring period as an abnormal extraction voltage curve segment; otherwise, mark the curve corresponding to this monitoring period as a normal extraction voltage curve segment. Obtain the extraction progress of the extraction voltage curve in any number of monitoring periods. If the extraction progress is lower than the extraction progress standard value, mark the curve corresponding to this monitoring period as an abnormal extraction progress curve segment; otherwise, mark the curve corresponding to this monitoring period as a normal extraction progress curve segment. Obtain the abnormal extraction voltage curve segment and the normal extraction progress curve segment in the same monitoring period, and calculate the Pearson correlation coefficient of the corresponding data of the abnormal extraction voltage curve segment and the normal extraction progress curve segment at the same moment. If the Pearson correlation coefficient is greater than 0.85, mark the abnormal extraction voltage curve segment and the normal extraction progress curve segment in the same monitoring period as mutually influencing abnormal curve segments. S4. Regulate the factors according to the factors that affect the extraction progress of soluble proteins. Specifically, the process of regulating the factors includes: Obtain the extraction progress of soluble proteins in the monitoring cycle corresponding to the extraction unqualified signal, and determine the compensation ratio. Multiply the compensation ratio by the average value of the relevant factor data in the monitoring cycle corresponding to the extraction unqualified signal to obtain the factor regulation value for the subsequent monitoring cycle; send the obtained factor regulation value for the subsequent monitoring cycle to the flash extractor and perform the work according to the regulated parameters. Among them, the process of determining the compensation ratio is: Calculate the deviation ratio of the extraction progress of soluble proteins in the monitoring cycle corresponding to the extraction unqualified signal to the extraction progress standard value, that is, through the following formula: ; In the formula, B represents the compensation ratio, D represents the extraction progress of soluble proteins in the monitoring cycle corresponding to the extraction unqualified signal, and D b represents the extraction progress standard value. Exemplarily, when the factor that affects the extraction progress of soluble proteins is the extraction voltage, obtain the extraction progress of soluble proteins in the monitoring cycle corresponding to the extraction unqualified signal, and determine the compensation ratio. Multiply the compensation ratio by the average value of the extraction voltage data in the monitoring cycle corresponding to the extraction unqualified signal to obtain the extraction voltage regulation value for the subsequent monitoring cycle.

[0022] Technical solution of the embodiment of the present invention: Obtain the soluble protein content during the extraction process of the fresh tobacco leaf filter residue solution in a flash extractor, calculate the soluble protein extraction progress, and evaluate the extraction status; generate an extraction instruction corresponding to the evaluated extraction status; when a detection instruction is obtained, obtain the relevant factor data under the monitoring period corresponding to the extraction unqualified signal, and perform an impact analysis with the soluble protein extraction progress to obtain the impact length and impact depth. Through comprehensive analysis of the impact length and impact depth, determine the factors that affect the soluble protein extraction progress; regulate the factors according to the factors that affect the soluble protein extraction progress. The present invention monitors the soluble protein content in real time during the extraction process, judges the extraction efficiency or progress of the soluble protein extract of fresh tobacco leaves, controls the extraction process in a timely and effective manner according to the judged result, optimizes the start-stop strategy, and can also identify the influencing factors of the extraction process according to the judged result. Under the determined influencing factors, regulate the factors in a timely manner to ensure the extraction efficiency of the soluble protein of fresh tobacco leaves.

[0023] Embodiment Two Based on the above Embodiment One, the extraction monitoring system includes the following modules: S1. Obtain the soluble protein content during the extraction process of the fresh tobacco leaf filter residue solution in a flash extractor, calculate the soluble protein extraction progress, and evaluate the extraction status; Specifically, the calculation process of the soluble protein extraction progress is as follows: Set the monitoring period. During the extraction process of the fresh tobacco leaf filter residue solution in the flash extractor, obtain the soluble protein content in different monitoring periods to obtain the soluble protein extraction progress; Compare the soluble protein extraction progress with the standard soluble protein content in different monitoring periods; If the soluble protein extraction progress is less than the standard soluble protein content in different monitoring periods, generate an extraction unqualified signal; If the soluble protein extraction progress is greater than or equal to the standard soluble protein content in different monitoring periods, generate an extraction qualified signal; S2. Generate an extraction instruction corresponding to the evaluated extraction status; Specifically, the generation process of the extraction instruction is as follows: When an extraction qualified signal is obtained, compare the soluble protein extraction progress with the standard soluble protein content in the final monitoring period. If it reaches, generate an extraction stop instruction; if it does not reach, generate an extraction continue instruction; It should be explained that: when an extraction stop instruction is obtained, it means that the soluble protein extraction work of the current fresh tobacco leaves is completed, and the current extraction work can be terminated. When an extraction continue instruction is obtained, it means that the soluble protein extraction work of the current fresh tobacco leaves is not completed and the extraction work needs to be continued; When an extraction unqualified signal is obtained, a detection instruction is generated; S3. When a detection instruction is obtained, obtain the relevant factor data under the monitoring period corresponding to the extraction unqualified signal, and conduct an impact analysis with the soluble protein extraction progress to obtain the impact length and impact depth. Through comprehensive analysis of the impact length and impact depth, determine the factors that affect the soluble protein extraction progress; Specifically, the calculation processes of the impact length and impact depth are as follows: Obtain the relevant factor data under the monitoring period corresponding to the extraction unqualified signal, and fit to obtain the relevant factor curve; among them, the relevant factor data includes the extraction rotation speed and extraction voltage; And obtain the soluble protein extraction progress under the monitoring period corresponding to the extraction unqualified signal, and fit to obtain the extraction progress curve; Divide the monitoring period into several monitoring time periods, conduct an abnormal similarity analysis on the relevant factor curve and the extraction progress curve, and obtain the mutually influential abnormal curve segments; Calculate the proportion of the total time of the mutually influential abnormal curve segments in the duration of the monitoring period to obtain the impact length; Obtain the relevant factor slope and extraction progress slope of the mutually influential abnormal curve segments, calculate the deviation mean value of the relevant factor slope and the extraction progress slope to obtain the impact depth; Calculate the ratio of the impact depth to the impact length to obtain the impact degree value; Compare the impact depth with the impact depth threshold; If the impact depth is greater than or equal to the impact depth threshold, then generate a non-impact signal; If the impact depth is less than the impact depth threshold, then generate an impact signal; It should be explained that: when an impact signal is generated, it means that this factor has a relatively high impact on the soluble protein extraction progress; when a non-impact signal is generated, it means that this factor does not have a relatively high impact on the soluble protein extraction progress; Among them, the process of the abnormal similarity analysis is as follows: First, conduct an abnormal analysis: obtain the mean value of the relevant factor data of the relevant factor curve in any monitoring time period. If the mean value of the relevant factor data is lower than the process standard value, then mark the curve corresponding to this monitoring time period as the relevant factor abnormal curve segment; otherwise, mark the curve corresponding to this monitoring time period as the relevant factor normal curve segment; Obtain the extraction progress of the relevant factor curve in any monitoring time period. If the extraction progress is lower than the extraction progress standard value, then mark the curve corresponding to this monitoring time period as the extraction progress abnormal curve segment; otherwise, mark the curve corresponding to this monitoring time period as the extraction progress normal curve segment; First, perform similarity analysis: Obtain the abnormal curve segments of relevant factors and the normal curve segments of extraction progress during the same monitoring period, calculate the Pearson correlation coefficient of the corresponding data of the abnormal curve segments of relevant factors and the normal curve segments of extraction progress at the same moment. If the Pearson correlation coefficient is greater than 0.85, mark the abnormal curve segments of relevant factors and the normal curve segments of extraction progress during the same monitoring period as mutually influential abnormal curve segments. S4. Regulate the factors according to the factors that affect the extraction progress of soluble proteins. Specifically, the process of regulating the factors includes: Obtain the extraction progress of soluble proteins during the monitoring period corresponding to the extraction unqualified signal, and determine the compensation ratio. Multiply the compensation ratio by the average value of the relevant factor data during the monitoring period corresponding to the extraction unqualified signal to obtain the factor regulation value for the subsequent monitoring period. Among them, the process of determining the compensation ratio is: Calculate the deviation ratio of the extraction progress of soluble proteins during the monitoring period corresponding to the extraction unqualified signal from the extraction progress standard value, that is, through the following formula: ; In the formula, B represents the compensation ratio, D represents the extraction progress of soluble proteins during the monitoring period corresponding to the extraction unqualified signal, and D b represents the extraction progress standard value.

[0024] The above has described a detailed example of the present invention, but the content described is only a preferred example of the present invention and cannot be considered as limiting the scope of implementation of the present invention. All equivalent changes and improvements made according to the scope of the present invention application should still fall within the scope covered by the patent of the present invention.

Claims

1. A method for extracting a soluble protein extract from fresh tobacco leaves, characterized in that: The following steps are involved: The decolorized and defatted fresh tobacco leaf residue is placed in a flash extractor with an extraction monitoring system for extraction and filtration; adding neutral salt to the supernatant until the salt solution is saturated, precipitating and drying to obtain a soluble protein extract of fresh tobacco leaves; The steps of extraction monitoring performed by the flash extractor are as follows: Obtain the soluble protein content of the fresh tobacco leaf residue solution during the extraction process in the flash extractor, calculate the soluble protein extraction progress, and evaluate the extraction status; If the extraction status is an extraction failure signal, a detection instruction is generated; If a detection instruction is received, the data of relevant factors in the monitoring period corresponding to the extraction failure signal are obtained, and the impact analysis is performed with the soluble protein extraction progress to obtain the impact length and impact depth. Through a comprehensive analysis of the impact length and impact depth, the factors affecting the soluble protein extraction progress are determined; Factors are regulated according to the factors that affect the progress of soluble protein extraction.

2. The method for extracting a soluble protein extract from fresh tobacco leaves according to claim 1, characterized in that: The calculation process of soluble protein extraction progress is: The monitoring cycle is set, and during the extraction process of the fresh tobacco leaf residue solution in the flash extractor, the soluble protein content in different monitoring cycles is obtained to obtain the soluble protein extraction progress.

3. The method for extracting a soluble protein extract from fresh tobacco leaves according to claim 2, characterized in that: The process of extracting the status assessment is: If the soluble protein extraction progress is less than the standard soluble protein content in different monitoring periods, an extraction failure signal is generated.

4. The method for extracting a soluble protein extract from fresh tobacco leaves according to claim 1, characterized in that: The calculation process of the impact length is: Obtain relevant factor data in the monitoring period corresponding to the extraction of unqualified signals, and obtain relevant factor curves by fitting; Obtaining the soluble protein extraction progress in the monitoring period corresponding to the extraction failure signal, and fitting to obtain the extraction progress curve; The monitoring cycle is divided into several monitoring periods, and the abnormal similarity analysis is performed on the relevant factor curve and the extraction progress curve to obtain the mutually influencing abnormal curve segments; The influence length is obtained by calculating the proportion of the total time of the mutually influencing abnormal curve segments in the monitoring period.

5. The method for extracting a soluble protein extract from fresh tobacco leaves according to claim 4, characterized in that: The calculation process of the impact depth is: Obtain the slope of the relevant factors and the extraction progress slope of the mutually influencing abnormal curve segment, calculate the mean deviation between the slope of the relevant factors and the slope of the extraction progress, and obtain the influence depth.

6. The method for extracting a soluble protein extract from fresh tobacco leaves according to claim 5, characterized in that: The process of determining the factors that affect the progress of soluble protein extraction is as follows: Calculate the ratio of the impact depth to the impact length to obtain the impact degree value; If the impact depth is less than the impact depth threshold, an impact signal is generated.

7. The method for extracting a soluble protein extract from fresh tobacco leaves according to claim 1, characterized in that: The process of anomaly similarity analysis includes: Obtain the mean value of the relevant factor data of the relevant factor curve in any monitoring period. If the mean value of the relevant factor data is lower than the process standard value, the curve corresponding to the monitoring period is marked as an abnormal curve segment of the relevant factor; The extraction progress of the relevant factor curve in any monitoring period is obtained. If the extraction progress is lower than the standard value of the extraction progress, the curve corresponding to the monitoring period is marked as an abnormal extraction progress curve segment.

8. The method for extracting a soluble protein extract from fresh tobacco leaves according to claim 7, characterized in that: The process of anomaly similarity analysis also includes: Obtain the abnormal curve segments of related factors and the normal curve segments of extraction progress in the same monitoring period, calculate the Pearson correlation coefficient of the corresponding data of the abnormal curve segments of related factors and the normal curve segments of extraction progress at the same time, and if the Pearson correlation coefficient is greater than the coefficient threshold, mark the abnormal curve segments of related factors and the normal curve segments of extraction progress in the same monitoring period as mutually influencing abnormal curve segments.

9. The method for extracting a soluble protein extract from fresh tobacco leaves according to claim 1, characterized in that: The process of regulating factors includes: Obtaining the soluble protein extraction progress in the monitoring period corresponding to the extraction failure signal, and determining the compensation ratio; The compensation ratio is multiplied by the mean value of the relevant factor data in the monitoring period corresponding to the extraction of the unqualified signal to obtain the factor control value of the subsequent monitoring period.

10. The method for extracting a soluble protein extract from fresh tobacco leaves according to claim 9, characterized in that: The process of determining the compensation ratio is as follows: Calculate the deviation ratio between the soluble protein extraction progress and the standard value of the extraction progress in the monitoring period corresponding to the extraction failure signal.

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