Multi-channel liquid chromatography-mass spectrometry device

Abstract

The application relates to a multi-channel liquid chromatography-mass spectrometry device, the multi-channel liquid chromatography-mass spectrometry device comprises a first switching valve, a mass spectrometer and a plurality of channels, the mass spectrometer and the plurality of channels are respectively connected with the first switching valve; each channel comprises a sample introduction module and a chromatographic separation module, and the first switching valve is used for enabling one channel to be communicated with the mass spectrometer, so that a sample can enter the chromatographic separation module through the corresponding sample introduction module and then enter the mass spectrometer through the first switching valve. According to the multi-channel liquid chromatography-mass spectrometry device provided by the invention, the sample injection starting time of each channel can be freely arranged, so that two adjacent channels can conveniently start sample injection at a smaller time interval, the two adjacent channels can perform sample peak appearance at a smaller time interval, and the efficiency of multiple channels is fully exerted.

Description

Technical Field

[0001] This application relates to the technical field of liquid chromatography-mass spectrometry (LC-MS) devices, and particularly to a multi-channel LC-MS device. Background Art

[0002] The balance of human biochemical metabolism is crucial for health. Metabolic disorders may lead to genetic diseases or chronic diseases such as hypertension, cardiovascular diseases, diabetes, and reproductive system diseases. The detection of biochemical metabolic markers is crucial for disease diagnosis and the evaluation of treatment responses. Although immunological methods are widely used, they have limitations. The liquid chromatography-mass spectrometry (LC-MS / MS) technology, with its high sensitivity and specificity, has become the gold standard for detecting small molecule biochemical markers and is widely used in clinical testing, showing great application potential.

[0003] Since clinical testing requires a large number of samples to be tested in a short time, many laboratories need to configure multiple sets of LC-MS devices to meet the experimental requirements. During the process of LC-MS, the chromatographic separation time is usually longer than the mass spectrometry acquisition time. For example, in the standard method for detecting 25-hydroxyvitamin D by liquid chromatography-tandem mass spectrometry, the entire chromatographic separation process for vitamin D2 and vitamin D3 takes 3 minutes, and the sample elution time is from 2.5 minutes to 3.0 minutes. Therefore, the mass spectrometer only needs to collect signals from 2.5 minutes to 3.0 minutes, and the rest of the time is idle.

[0004] To improve the sample testing throughput, a multi-channel LC-MS device is usually used for detection. Common multi-channel LC-MS devices include an injector, a sample loop, multiple chromatographic columns, and switching valves. The injector sucks the sample into the sample loop, and the sample in the sample loop is pushed into one of the chromatographic columns through the switching valve and the solvent pump, and then the chromatographic column is connected to the mass spectrometer through the switching valve. In this way, the injector, the sample loop, and one of the chromatographic columns are connected to form a channel. Since a single injector is shared among multiple channels, and each sample suction process of the injector takes dozens of seconds, that is, the minimum time interval between the start of sample injection for adjacent two channels is at least the time required for the injector to complete one sample suction. Therefore, it is difficult to keep the sample injection time of each channel at a small interval, and thus the efficiency of the multi-channel cannot be fully utilized. Summary of the Invention

[0005] Based on this, it is necessary to provide a multi-channel LC-MS device to solve the problem that the working efficiency of the multi-channel cannot be fully utilized due to being restricted by the sample suction time of the injector.

[0006] A multi-channel liquid chromatography-mass spectrometry device. The multi-channel liquid chromatography-mass spectrometry device includes a first switching valve, a mass spectrometer, and multiple channels. The mass spectrometer and the multiple channels are respectively connected to the first switching valve. Each channel includes an injection module and a chromatographic separation module respectively. The first switching valve is used to connect one of the channels to the mass spectrometer, so that the sample can enter the chromatographic separation module through the corresponding injection module, and then enter the mass spectrometer through the first switching valve.

[0007] In one embodiment, each chromatographic separation module includes a second switching valve and two chromatographic columns arranged in parallel. The second switching valve is used to connect one of the chromatographic columns between the injection module and the first switching valve.

[0008] In one embodiment, the second switching valve has ports A1, B1, C1, D1, E1, and F1. One of the chromatographic columns is connected to ports A1 and B1, and the other chromatographic column is connected to ports D1 and E1. Port C1 is connected to the injection module, and port F1 is connected to the first switching valve. Among them, the second switching valve has state one and state two. When the second switching valve is in state one, port A1 is connected to port F1, port B1 is connected to port C1, and port D1 is connected to port E1. When the second switching valve is in state two, port A1 is connected to port B1, port C1 is connected to port D1, and port E1 is connected to port F1.

[0009] In one embodiment, each injection module includes a sampler, a solvent pump, and a third switching valve. The sampler has a first end and a second end. The first end is connected to the third switching valve, and the second end is used to aspirate the sample or to connect to the chromatographic separation module. The solvent pump is connected to the third switching valve. Among them, the third switching valve can connect or disconnect the solvent pump from the first end.

[0010] In one embodiment, each injection module further includes a needle seat, and the needle seat has an inlet and an outlet. The third switching valve has ports A2, B2, C2, D2, E2, and F2. The solvent pump is connected to port B2, the first end is connected to port C2, the second end is detachably installed at the inlet, and the outlet is connected to port F2. Among them, the third switching valve has state one and state two. When the third switching valve is in state one, port A2 is connected to port F2, port B2 is connected to port C2, and port D2 is connected to port E2. When the third switching valve is in state two, port A2 is connected to port B2, port C2 is connected to port D2, and port E2 is connected to port F2.

[0011] In one embodiment, the number of channels is configured to be two. The two channels are the first channel and the second channel respectively. The first switching valve has ports A3, B3, C3, D3, E3 and F3. The mass spectrometer is connected to port A3, the first channel is connected to port B3, and the second channel is connected to port F3. Among them, the first switching valve has state one and state two. When the first switching valve is in state one, port A3 is communicated with port F3, port B3 is communicated with port C3, and port D3 is communicated with port E3. When the first switching valve is in state two, port A3 is communicated with port B3, port C3 is communicated with port D3, and port E3 is communicated with port F3.

[0012] In one embodiment, the first channel has a sampling mode. When the first channel is in the sampling mode, the third switching valve of the first channel is in state two, and the first switching valve is in state one. The second channel has a sampling mode. When the second channel is in the sampling mode, the third switching valve of the second channel is in state two, and the first switching valve is in state two.

[0013] In one embodiment, the first channel has a working mode. When the first channel is in the working mode, the third switching valve of the first channel is in state one, and the first switching valve is in state two. The second channel has a working mode. When the second channel is in the working mode, the third switching valve of the second channel is in state one, and the third switching valve is in state one.

[0014] In one embodiment, the multi-channel liquid chromatography-mass spectrometry device further includes a transmission mechanism and a sample bottle. The sample bottle is used for storing samples, and the transmission mechanism is used to drive the sampler to insert into the sample bottle.

[0015] In one embodiment, multiple samplers are spaced along a first direction, and the sample bottles are spaced from the multiple samplers in a second direction, where the first direction is perpendicular to the second direction.

[0016] Compared with the prior art, in the multi-channel liquid chromatography-mass spectrometry device provided by the present application, since each channel has an injection module and a chromatographic separation module, each channel can work completely independently. Specifically, the injection module in each channel can inject samples independently, and then send the samples to the chromatographic separation module for chromatographic separation. Therefore, the injection time of each channel can be freely arranged to facilitate adjacent two channels to start injection at a smaller time interval, so that adjacent two channels can have sample peaks at a smaller time interval, thereby giving full play to the efficiency of the multi-channel. Description of the Drawings

[0017] To more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present application. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0018] Figure 1 It is a system block diagram of a multi-channel liquid chromatography-mass spectrometry device in one of the embodiments provided by the present application; Figure 2 It is a schematic diagram of the relative positions of multiple samplers and sample bottles provided by the present application; Figure 3 It is a schematic diagram of the first channel in the sample injection mode in one of the embodiments provided by the present application; Figure 4 It is a schematic diagram of the first channel in the working mode in one of the embodiments provided by the present application; Figure 5 It is a schematic diagram of the second channel in the sample injection mode in one of the embodiments provided by the present application; Figure 6 It is a schematic diagram of the second channel in the working mode in one of the embodiments provided by the present application.

[0019] Reference numerals: 100, multi-channel liquid chromatography-mass spectrometry device; 10, first switching valve; 20, mass spectrometer; 30, channel; 310, first channel; 320, second channel; 31, sample injection module; 311, sampler; 301, first end; 302, second end; 312, solvent pump; 313, third switching valve; 314, needle seat; 315, syringe; 304, inlet; 305, outlet; 32, chromatographic separation module; 321, second switching valve; 322, chromatographic column; 50, sample bottle; 60, waste liquid pool. Detailed Embodiments

[0020] To make the above objects, features, and advantages of the present application more obvious and understandable, the following will describe the detailed embodiments of the present application in conjunction with the drawings. Many specific details are set forth in the following description to fully understand the present application. However, the present application can be implemented in many other ways different from those described herein. Those skilled in the art can make similar improvements without departing from the connotation of the present application. Therefore, the present application is not limited by the specific embodiments disclosed below.

[0021] It should be noted that when a component is referred to as "fixed to" or "disposed on" another component, it can be directly on the other component or there may also be an intermediate component. When a component is considered to be "connected" to another component, it can be directly connected to the other component or there may be an intermediate component at the same time. The terms "vertical", "horizontal", "upper", "lower", "left", "right" and similar expressions used in the description of this application are only for illustrative purposes and do not represent the only implementation.

[0022] In addition, the terms "one" and "two" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, the features defined with "one" and "two" may explicitly or implicitly include at least one of such features. In the description of this application, the meaning of "a plurality" is at least two, such as two, three, etc., unless otherwise specifically defined.

[0023] In this application, unless otherwise clearly specified and defined, a feature on a second feature "above" or "below" may be that the first feature is in direct contact with the second feature, or the first feature is indirectly in contact with the second feature through an intermediate medium. Moreover, a feature "above", "over" and "on top of" a second feature may be that the first feature is directly above or obliquely above the second feature, or only indicates that the horizontal height of the first feature is higher than that of the second feature. A feature "below", "beneath" and "underneath" a second feature may be that the first feature is directly below or obliquely below the second feature, or only indicates that the horizontal height of the first feature is less than that of the second feature.

[0024] Unless otherwise defined, all technical and scientific terms used in the description of this application have the same meaning as commonly understood by those skilled in the technical field to which this application belongs. The terms used in the description of this application are only for the purpose of describing specific implementations and are not intended to limit this application. The term "and / or" used in the description of this application includes any and all combinations of one or more of the related listed items.

[0025] Please refer to Figure 1 , this application provides a multi-channel liquid chromatography-mass spectrometry device 100. The multi-channel liquid chromatography-mass spectrometry device 100 includes a first switching valve 10, a mass spectrometer 20, and a plurality of channels 30. The mass spectrometer 20 and the plurality of channels 30 are respectively connected to the first switching valve 10. Each channel 30 respectively includes an injection module 31 and a chromatographic separation module 32. The first switching valve 10 is used to connect one of the channels 30 to the mass spectrometer 20, so that the sample can enter the chromatographic separation module 32 through the corresponding injection module 31, and then enter the mass spectrometer 20 through the first switching valve 10.

[0026] The multi-channel liquid chromatography-mass spectrometry device 100 provided by the present application. Since each channel 30 is equipped with a sampling module 31 and a chromatographic separation module 32, each channel 30 can work completely independently. Specifically, the sampling module 31 in each channel 30 can sample independently and then send the sample into the chromatographic separation module 32 for chromatographic separation. Therefore, the sampling time of each channel 30 can be freely arranged. For example, multiple sampling modules 31 can start sampling at smaller time intervals and be used alternately, so that the sample peaks in adjacent two channels 30 occur at smaller time intervals, giving full play to the efficiency of the multi-channel. Or, multiple sampling modules 31 can also start sampling simultaneously.

[0027] It should be noted that in the multi-channel liquid chromatography-mass spectrometry device 100 provided by the present application, the mass spectrometer 20 can also be replaced with other detectors. For example, the mass spectrometer 20 can be replaced with an ultraviolet fluorescence detector.

[0028] Optionally, adjacent two channels 30 start sampling at a time interval of T1, where T1 is the time required for the mass spectrometer 20 to complete one sampling.

[0029] Exemplarily, when the multi-channel liquid chromatography-mass spectrometry device 100 is used for detecting the 25-hydroxyvitamin D standardization method. The number of channels 30 is configured to be six. By switching the first switching valve 10, one of the channels 30 is connected to the mass spectrometer 20 through the first switching valve 10. When each channel 30 is connected to the mass spectrometer 20, the entire process of chromatographic separation is 3 minutes; the sample peak time of each chromatographic separation module 32 starts from 2.5 minutes to 3.0 minutes, and the time required for the mass spectrometer 20 to complete one sampling is 0.5 minutes, that is, T1 = 0.5 minutes. By making adjacent two channels 30 start sampling at a time interval of 0.5 minutes, the throughput of sample testing can be increased to 6 times the original, that is, the effect of the multi-channel can be fully exerted, and the working efficiency of the multi-channel liquid chromatography-mass spectrometry device 100 can be improved.

[0030] Wherein, each chromatographic separation module 32 includes a second switching valve 321 and two chromatographic columns 322 arranged in parallel. The second switching valve 321 is used to connect one of the chromatographic columns 322 between the sampling module 31 and the first switching valve 10. When the chromatographic column 322 is connected between the sampling module 31 and the first switching valve 10, the sample can flow through the chromatographic column 322 from the sampling module 31 for chromatographic separation. By setting the second switching valve 321, only one chromatographic column 322 performs chromatographic separation in each chromatographic separation module 32, and the other chromatographic column 322 is used as a standby. In this way, when one of the chromatographic columns 322 is damaged, the standby chromatographic column 322 can be connected between the sampling module 31 and the first switching valve 10 by switching the second switching valve 321, so that the chromatographic separation module 32 can work continuously.

[0031] Optionally, in one embodiment, the second switching valve 321 has ports A1, B1, C1, D1, E1 and F1. One chromatographic column 322 is connected to ports A1 and B1, and the other chromatographic column 322 is connected to ports D1 and E1. Port C1 is connected to the injection module 31, and port F1 is connected to the first switching valve 10. The second switching valve 321 has state one and state two. When the second switching valve 321 is in state one, port A1 communicates with port F1, port B1 communicates with port C1, and port D1 communicates with port E1. When the second switching valve 321 is in state two, port A1 communicates with port B1, port C1 communicates with port D1, and port E1 communicates with port F1. That is, in this embodiment, the second switching valve 321 is a six-way valve. When the second switching valve 321 is in state one, one chromatographic column 322 is connected between the injection module 31 and the first switching valve 10. When the second switching valve 321 is in state two, the other chromatographic column 322 is connected between the injection module 31 and the first switching valve 10.

[0032] Each injection module 31 includes a sampler 311, a solvent pump 312, and a third switching valve 313. The sampler 311 has a first end 301 and a second end 302. The first end 301 is connected to the third switching valve 313, and the second end 302 is used to aspirate a sample or to connect to the chromatographic separation module 32. The solvent pump 312 is connected to the third switching valve 313. The third switching valve 313 can connect or disconnect the solvent pump 312 from the first end 301.

[0033] When the sampler 311 aspirates a sample through the second end 302, the third switching valve 313 disconnects the solvent pump 312 from the first end 301. In this way, it is possible to prevent the solvent in the solvent pump 312 from pushing out the sample in the sampler 311. When the second end 302 is connected to the chromatographic separation module 32 and the third switching valve 313 connects the solvent pump 312 to the first end 301, the solvent in the solvent pump 312 pushes the sample in the sampler 311 to the chromatographic separation module 32.

[0034] Furthermore, each injection module 31 further includes a needle seat 314. The needle seat 314 has an inlet 304 and an outlet 305. The third switching valve 313 has ports A2, B2, C2, D2, E2 and F2. The solvent pump 312 is connected to port B2, the first end 301 is connected to port C2, the second end 302 is detachably installed at the inlet 304, and the outlet 305 is connected to port F2. The third switching valve 313 has state one and state two. When the third switching valve 313 is in state one, port A2 communicates with port F2, port B2 communicates with port C2, and port D2 communicates with port E2. When the third switching valve 313 is in state two, port A2 communicates with port B2, port C2 communicates with port D2, and port E2 communicates with port F2.

[0035] That is, the third switching valve 313 is configured as a six-way valve. When the third switching valve 313 is in the first state and the second end 302 is installed at the inlet 304, the solvent pump 312 is connected to the first end 301, and the second end 302 is connected to the chromatographic separation module 32 through the needle seat 314. The solvent in the solvent pump 312 pushes the sample in the sampler 311 to the chromatographic separation module 32. During this process, the solvent cleans the sampler 311, thus saving the cleaning time of the sampler 311. And compared with the related structure that needs to set a sample loop, each injection module 31 in this application cancels the sample loop, which also saves the cleaning time of the sample loop. When the third switching valve 313 is in the second state, the solvent pump 312 is connected to the chromatographic separation module 32, and the solvent in the solvent pump 312 enters the chromatographic separation module 32 through the B2 port and the A2 port. At this time, the second end 302 is disassembled from the inlet 304 and is used to aspirate the sample. In this way, the third switching valve 313 can not only be used to connect or disconnect the solvent pump 312, but also be used to connect or disconnect the needle seat 314 and the chromatographic separation module 32.

[0036] Further, as Figure 3 shown, the A2 port is connected to the C1 port, and the D2 port is connected to a syringe 315. In this way, when the third switching valve 313 is in the second state, the syringe 315, the D2 port, the C2 port, the first end 301, and the second end 302 are connected in sequence. The syringe 315 provides a suction force, so that the sample enters the sampler 311 through the second end 302.

[0037] The multi-channel liquid chromatography-mass spectrometry device 100 further includes a transmission mechanism and a sample bottle 50. The sample bottle 50 is used to store samples, and the transmission mechanism is used to drive the sampler 311 to insert into the sample bottle 50.

[0038] Further, as Figure 2 shown, a plurality of samplers 311 are spaced apart along the first direction X, and the sample bottle 50 is spaced apart from the plurality of samplers 311 in the second direction Y, wherein the first direction X and the second direction Y are perpendicular to each other. In this way, when it is necessary to connect a certain channel 30 to the mass spectrometer 20, the transmission mechanism first moves the sampler 311 in this channel 30 along the first direction X so that this sampler 311 faces the sample bottle 50 along the second direction Y. Then, the transmission mechanism moves this sampler 311 along the second direction Y so that the corresponding second end 302 is inserted into the sample bottle 50 to aspirate the sample.

[0039] Exemplarily, in one embodiment, as Figure 1As shown, the number of channels 30 is configured to be two, and the first switching valve 10 is configured as a six-way valve. Specifically, the two channels 30 are the first channel 310 and the second channel 320 respectively. The first switching valve 10 has ports A3, B3, C3, D3, E3, and F3. The mass spectrometer 20 is connected to port A3, the first channel 310 is connected to port B3, and the second channel 320 is connected to port F3. Among them, the first switching valve 10 has state one and state two. When the first switching valve 10 is in state one, port A3 is connected to port F3, port B3 is connected to port C3, and port D3 is connected to port E3. When the first switching valve 10 is in state two, port A3 is connected to port B3, port C3 is connected to port D3, and port E3 is connected to port F3. It can be understood that when the first switching valve 10 is in state one, the second channel 320 is connected to the mass spectrometer 20; when the first switching valve 10 is in state two, the first channel 310 is connected to the mass spectrometer 20.

[0040] Among them, the F1 port of the first channel 310 is connected to port B3, the F1 port of the second channel 320 is connected to port F3, and the C1 port and the E1 port are respectively connected to the waste liquid pool 60.

[0041] However, it is not limited thereto. In other embodiments, the number of channels 30 may also be three, four, or five, etc., and the first switching valve 10 is configured as a corresponding multi-way valve or a combination of multi-way valves.

[0042] Taking the number of channels 30 configured to be two as an example, the working mode of each channel of the multi-channel liquid chromatography-mass spectrometry device will be introduced below.

[0043] Sampling mode of the first channel 310 Please refer to Figure 1 and Figure 3 , when the first channel 310 is in the sampling mode, the third switching valve 313 of the first channel 310 is in state two, and the first switching valve 10 is in state one. At this time, the flow path of the sample is: the second end 302 - the first end 301 - port C3 - port D3 - the syringe 315. The flow path of the solvent is: the solvent pump 312 - port B3 - port A3 - port C2 - the chromatographic separation module 32 - port F2 - port B1 - port C1 - the waste liquid pool 60.

[0044] Working mode of the first channel 310 Please refer to Figure 1 and Figure 4, when the first channel 310 is in the working mode, the third switching valve 313 of the first channel 310 is in state one, and the first switching valve 10 is in state two. At this time, the flow path of the solvent is as follows: from the B3 port of the solvent pump 312, through the C3 port, to the first end 301, and then into the sampler 311. Then, it pushes the sample along the second end 302, through the inlet 304, the outlet 305, the F3 port, the A2 port, the C2 port, into the chromatographic separation module 32, through the F2 port, the B1 port, the A1 port, and then to the mass spectrometer 20.

[0045] Sampling mode of the second channel 320 Please refer to Figure 1 and Figure 5 , when the second channel 320 is in the sampling mode, the third switching valve 313 of the second channel 320 is in state two, and the first switching valve 10 is in state two. At this time, the flow path of the sample is as follows: from the second end 302, through the first end 301, the C3 port, the D3 port, and then to the syringe 315. The flow path of the solvent is as follows: from the B3 port of the solvent pump 312, through the A3 port, the C2 port, into the chromatographic separation module 32, through the F2 port, the F1 port, the E1 port, and then to the waste liquid pool 60.

[0046] Working mode of the second channel 320 Please refer to Figure 1 and Figure 6 , when the second channel 320 is in the working mode, the third switching valve 313 of the second channel 320 is in state one, and the third switching valve 313 is in state one. At this time, the flow path of the solvent is as follows: from the B3 port of the solvent pump 312, through the C3 port, to the first end 301, and then into the sampler 311. Then, it pushes the sample along the second end 302, through the inlet 304, the outlet 305, the F3 port, the A2 port, the C2 port, into the chromatographic separation module 32, through the F2 port, the F1 port, the A1 port, and then to the mass spectrometer 20.

[0047] The technical features of the above-described embodiments can be combined arbitrarily. For the sake of brevity of description, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, it should be considered as the scope described in this specification.

[0048] The above-described embodiments only represent several implementation manners of the present application. The description is relatively specific and detailed, but it should not be construed as a limitation on the scope of the patent application. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present application, several modifications and improvements can still be made, and these all belong to the protection scope of the present application. Therefore, the patent protection scope of the present application should be subject to the appended claims.

Claims

1. A multi-channel liquid-mass spectrometry device, characterized in that: The multi-channel liquid-mass spectrometry device (100) comprises a first switching valve (10), a mass spectrometer (20), and a plurality of channels (30), wherein the mass spectrometer (20) and the plurality of channels (30) are respectively connected to the first switching valve (10); Each of the channels (30) comprises a sample injection module (31) and a chromatographic separation module (32), and the first switching valve (10) is used to connect one of the channels (30) to the mass spectrometer (20), so that the sample can enter the chromatographic separation module (32) through the corresponding sample injection module (31), and then enter the mass spectrometer (20) through the first switching valve (10).

2. The multi-channel liquid chromatography-mass spectrometry device according to claim 1, characterized in that: Each of the chromatographic separation modules (32) comprises a second switching valve (321) and two chromatographic columns (322) arranged in parallel, wherein the second switching valve (321) is used to connect one of the chromatographic columns (322) between the injection module (31) and the first switching valve (10).

3. The multi-channel liquid chromatography-mass spectrometry device according to claim 2, characterized in that: The second switching valve (321) has an A1 port, a B1 port, a C1 port, a D1 port, an E1 port and an F1 port, one of the chromatographic columns (322) is connected to the A1 port and the B1 port, another of the chromatographic columns (322) is connected to the D1 port and the E1 port, the C1 port is connected to the injection module (31), and the F1 port is connected to the first switching valve (10); The second switching valve (321) has state one and state two. When the second switching valve (321) is in state one, the A1 port is connected to the F1 port, the B1 port is connected to the C1 port, and the D1 port is connected to the E1 port. When the second switching valve (321) is in state two, the A1 port is connected to the B1 port, the C1 port is connected to the D1 port, and the E1 port is connected to the F1 port.

4. The multi-channel liquid chromatography-mass spectrometry device according to claim 3, characterized in that: Each of the injection modules (31) comprises a sampler (311), a solvent pump (312) and a third switching valve (313); the sampler (311) has a first end (301) and a second end (302); the first end (301) is connected to the third switching valve (313); the second end (302) is used to absorb a sample or to connect to the chromatographic separation module (32); and the solvent pump (312) is connected to the third switching valve (313); The third switching valve (313) is capable of connecting or disconnecting the solvent pump (312) and the first end (301).

5. The multi-channel liquid chromatography-mass spectrometry device according to claim 4, characterized in that: Each of the injection modules (31) further comprises a needle seat (314), and the needle seat (314) has an inlet (304) and an outlet (305), the third switching valve (313) has an A2 port, a B2 port, a C2 port, a D2 port, an E2 port and an F2 port, the solvent pump (312) is connected to the B2 port, the first end (301) is connected to the C2 port, the second end (302) is detachably mounted on the inlet (304), and the outlet (305) is connected to the F2 port; The third switching valve (313) has state one and state two. When the third switching valve (313) is in state one, the A2 port is connected to the F2 port, the B2 port is connected to the C2 port, and the D2 port is connected to the E2 port. When the third switching valve (313) is in state two, the A2 port is connected to the B2 port, the C2 port is connected to the D2 port, and the E2 port is connected to the F2 port.

6. The multi-channel liquid chromatography-mass spectrometry device according to claim 5, characterized in that: The number of the channels (30) is configured to be two, the two channels (30) are respectively a first channel (310) and a second channel (320), the first switching valve (10) has an A3 port, a B3 port, a C3 port, a D3 port, an E3 port and an F3 port, the mass spectrometer (20) is connected to the A3 port, the first channel (310) is connected to the B3 port, and the second channel (320) is connected to the F3 port; The first switching valve (10) has a state one and a state two. When the first switching valve (10) is in state one, the A3 port is connected to the F3 port, the B3 port is connected to the C3 port, and the D3 port is connected to the E3 port. When the first switching valve (10) is in state two, the A3 port is connected to the B3 port, the C3 port is connected to the D3 port, and the E3 port is connected to the F3 port.

7. The multi-channel liquid chromatography-mass spectrometry device according to claim 6, characterized in that: The first channel (310) has an injection mode, and when the first channel (310) is in the injection mode, the third switching valve (313) of the first channel (310) is in state two, and the first switching valve (10) is in state one; The second channel (320) has an injection mode. When the second channel (320) is in the injection mode, the third switching valve (313) of the second channel (320) is in state two, and the first switching valve (10) is in state two.

8. The multi-channel liquid chromatography-mass spectrometry device according to claim 6, characterized in that: The first channel (310) has a working mode, and when the first channel (310) is in the working mode, the third switching valve (313) of the first channel (310) is in state one, and the first switching valve (10) is in state two; The second channel (320) has a working mode. When the second channel (320) is in the working mode, the third switching valve (313) of the second channel (320) is in state one, and the third switching valve (313) is in state one.

9. The multi-channel liquid chromatography-mass spectrometry device according to claim 6, characterized in that: The multi-channel LC-MS device (100) further comprises a transmission mechanism and a sample bottle (50), wherein the sample bottle (50) is used to store samples, and the transmission mechanism is used to drive the sampler (311) to be inserted into the sample bottle (50).

10. The multi-channel liquid chromatography-mass spectrometry device according to claim 9, characterized in that: The plurality of samplers (311) are spaced apart along a first direction, and the sample bottle (50) is spaced apart from the plurality of samplers (311) in a second direction, wherein the first direction and the second direction are perpendicular to each other.

Images