Organic liquid fertilizer containing acetylisovaleryltylosin tartrate finished product liquid and preparation process thereof

Abstract

The invention belongs to the field of organic liquid fertilizers, and particularly relates to an organic liquid fertilizer containing an acetylisovaleryltylosin tartrate finished product liquid and a preparation process thereof. Comprising the following steps: diluting dry powder of acetylisovaleryltylosin tartrate according to a mass ratio of 1: (100-500) to obtain finished product liquid of acetylisovaleryltylosin tartrate; performing aerobic culture in a liquid culture medium containing starch, yeast powder, soybean meal, yeast extract and inorganic salt, and controlling the fermentation time to be 36-110 hours to obtain acetylisovaleryltylosin tartrate fermentation liquor; the preparation method comprises the following steps: putting modified medical stone, humic acid, acetylisovaleryltylosin tartrate fermentation liquor, pyrethrin, brown sugar, sophocarpidine, honey, cinnabar, alum, ethylicin, stemona japonica, hymexazol, camphor and water into a fermentation tank according to the proportion, fully stirring at the temperature of 20-30 DEG C twice a day, fermenting for 7-10 days, and then fermenting for 7 days to obtain the organic liquid fertilizer containing the acetylisovaleryltylosin tartrate finished product liquid. The organic liquid fertilizer containing the acetylisovaleryltylosin tartrate finished product liquid can effectively improve the growth of crops.

Description

Technical Field

[0001] The present invention belongs to the field of organic liquid fertilizers, and specifically relates to an organic liquid fertilizer containing tylvalosin finished product liquid and a preparation process thereof. Background Art

[0002] Liquid compound fertilizer (Fluid Fertilizer), also known as fluid compound fertilizer, commonly known as liquid fertilizer, is a liquid product containing two or more nutrient elements required by crops. These nutrient elements are dissolved in water as solutes to form a solution or suspended in water as a suspension paste with the help of a suspending agent. Since the development of liquid fertilizers to date, there are many varieties, which can be roughly divided into two categories: liquid nitrogen fertilizers and liquid compound fertilizers. The effective substances in liquid nitrogen fertilizers include ammonium, nitrate and amide nitrogen, such as liquid ammonia, ammonia water, and nitrogen solution. Liquid compound fertilizers contain all the nutrient elements required for plant growth, such as nitrogen, phosphorus, potassium, calcium, magnesium, sulfur and trace elements, and organic substances soluble in water (such as humic acid, amino acid, plant growth regulator, etc.) can also be added. Liquid compound fertilizers can design fertilizer formulas according to the nutrient requirements of crop growth and adjust the fertilizer formulas at any time according to the different growth trends of crops. A scientific formula can significantly improve the fertilizer utilization rate, and the fertilizer efficiency of liquid fertilizers is usually more than 30% higher than that of conventional compound fertilizers.

[0003] Tylvalosin, also known as super tylosin, has the chemical name of 3-O-acetyl-4”-O-isovaleryl tylosin, and is a special antibiotic for livestock and poultry. Tylvalosin is a new macrolide antibiotic obtained by biotransformation of tylosin by Streptomyces thermotolerans and acetylation at the 3-position and isovalerylation at the 4”-position. Due to the change in its chemical structure, it has obvious advantages compared with tylosin in terms of antibacterial spectrum, antibacterial activity, drug resistance, chemical stability, oral absorption and blood drug concentration.

[0004] Organic liquid fertilizers have characteristics such as comprehensive nutrition, long-lasting fertilizer efficiency, improving soil structure, promoting the reproduction of microorganisms and enhancing the soil's fertilizer retention capacity, which can make up for the deficiencies of chemical fertilizers.

[0005] CN101774842A discloses an organic liquid fertilizer containing biomass active extraction components and a preparation method thereof. The organic liquid fertilizer of the biomass active extraction solution contains 1-200 g of potassium salt, 0-50 g of copper salt, 1.5-25 g of ferrous salt, and 1-30 g of boric acid. This organic liquid fertilizer can not only provide comprehensive nutrition for plants, but also has the effects of antibacterial and bactericidal, insect repellent and insecticidal, and regulating plant growth. However, potassium salt, copper salt and ferrous salt need to be added additionally in this organic liquid fertilizer, resulting in a higher cost.

[0006] CN107986888A discloses an organic liquid fertilizer based on the application of Chinese medicinal materials. The organic liquid fertilizer includes sodium polyacrylate, organic sugar alcohols, an active aqueous solution, and amino acid bacillus. Using the organic liquid fertilizer provided by this method can improve the ability of Chinese herbal medicines to resist adverse environments and photosynthesis, regulate the endocrine of Chinese herbal medicines, improve the soil, and degrade the residues of chemical fertilizers and pesticides. However, this organic liquid fertilizer requires a large amount of Chinese medicinal materials and has a high cost, which is not conducive to actual production applications.

[0007] CN105948959A discloses a rice straw nitrogen-fixing, phosphorus-dissolving, potassium-dissolving, insect-resistant, selenium-rich organic microbial liquid fertilizer. This method mixes the fermented and boiled concentrated solution of rice straw, the fermented and boiled concentrated solution of Cordyceps militaris mycelium, the nitrogen-fixing bacteria solution of rice straw, the phosphorus-dissolving and potassium-dissolving bacteria solution of rice straw, and the insect-resistant bacteria solution of rice straw in equal or unequal amounts. This method turns waste straw into treasure and can promote crop growth, metabolism, and insect resistance.

[0008] CN105724320A discloses a method for culturing earthworms using blue-green algae. This method drains the blue-green algae, adjusts the pH value to 6.2 - 6.8 with citric acid to make acidic blue-green algae; dries and cuts the crop straw; mixes the acidic blue-green algae and the cut straw segments evenly to obtain a mixture, and adds lactic acid bacteria, EM stock solution, and yeast to the mixture; composts, turning it once every 6 days, and the composting ends in 15 - 20 days to obtain a culture substrate for culturing earthworms. Although the above two methods apply blue-green algae and straw, the utilization rate is limited. Summary of the Invention

[0009] In order to make up for the deficiencies of the prior art and solve at least one technical problem proposed in the background art, such as the problem that if the content of a certain or certain nutrient elements is insufficient or excessive after using the compound liquid fertilizer, it may cause the photosynthesis of crops to be blocked.

[0010] The technical solution adopted by the present invention to solve its technical problems is: The preparation process of an organic liquid fertilizer containing tylvalosin finished liquid of the present invention includes the following steps: Step 1: Dilute tylvalosin dry powder according to a mass ratio of 1:100 - 500 to obtain tylvalosin finished liquid; Step 2: Aerobically culture in a liquid medium containing starch, yeast powder, soybean powder, yeast extract, and inorganic salts. During the fermentation process of tylvalosin, the pH will rise to about 7.5 at the initial stage of fermentation. First, add an appropriate amount of acetic acid to regulate the pH. When the pH drops to 7.3, start to feed and add a mixture of substrate tylosin dry powder and inducer. After adding this mixture, the pH starts to rise. It is necessary to add an appropriate amount of glucose solution to regulate the pH during the fermentation process to keep it always controlled within 7.00 - 7.30, and the fermentation time is controlled within 36 - 110h to obtain tylvalosin fermentation broth; Step 3: Put modified picrite, humic acid, tylvalosin fermentation broth, pyrethrins, brown sugar, matrine, honey, cinnabar, alum, ethyl garlicin, centipeda minima, hymexazol, camphor and water into a fermentation tank according to the above ratios, stir well, keep the temperature within 20 - 30 °C, stir twice a day, and after fermenting for 7 - 10 days, after fermenting for 7 days, an organic liquid fertilizer containing the finished tylvalosin liquid is obtained.

[0011] As a further scheme of the present invention: tylosin dry powder : L - leucine : L - valine = 10 : 2 : 1 (w:w:w).

[0012] As a further scheme of the present invention: In Step 3, the weight ratio of modified picrite, humic acid, tylvalosin fermentation broth, pyrethrins, brown sugar, matrine, honey, cinnabar, alum, ethyl garlicin, centipeda minima, hymexazol, camphor and water is 100 - 1000 : 4 - 40 : 20 - 50 : 0.5 - 5 : 1.5 - 15 : 1 - 10 : 1.2 - 12 : 1.5 - 15 : 1.2 - 12 : 1.1 - 11 : 1.2 - 12 : 2 - 20 : 1.1 - 11 : 1000 - 3000.

[0013] As a further scheme of the present invention: In Step 3, the preparation process of modified picrite includes the following steps: Take picrite, clean the surface, and perform preliminary crushing after drying. Transfer it to a muffle furnace and roast it at 300 - 400 °C for 1 - 2 h. Take it out after cooling, add 20 - 30 vt% hydrochloric acid solution, and soak for 3 - 5 h. Wash with deionized water and dry in an oven. Put it into a stirred ball mill and grind for 10 - 25 h. Among them, the grinding medium is steel balls, the mass ratio of balls to materials is 6 : 1, and the rotational speed of the ball mill is 400 - 450 r / min. Transfer it to a muffle furnace and roast it at 300 - 400 °C for 60 - 90 min. After taking it out, add 5 - 10 wt% cetyltrimethylammonium bromide solution and polyethylene glycol, and stir for 30 - 50 min. Filter and dry. Evenly spread it on the ground electrode in a low - temperature plasma treatment device, and treat it for 10 - 15 min under the conditions of a power frequency of 10 - 15 kHz, a working voltage of 20 kV, and a discharge power of 70 - 80 W. After taking it out, add mannitol, transfer it to an ultrasonic instrument and ultrasonicate for 20 - 30 min. Filter and dry to obtain modified picrite powder.

[0014] As a further solution of the present invention: the solid-liquid mass ratio of the medical stone powder and the cetyltrimethylammonium bromide solution is 1:5 - 10.

[0015] As a further solution of the present invention: the solid-liquid mass ratio of the medical stone powder and polyethylene glycol is 1:2 - 8.

[0016] As a further solution of the present invention: the solid-liquid mass ratio of the medical stone powder and polyethylene glycol is 1:2 - 8.

[0017] As a further solution of the present invention: the particle size of the modified medical stone powder ≤ 10 μm.

[0018] As a further solution of the present invention: the solid-liquid mass ratio of the medical stone powder and mannitol is 1:3.

[0019] An organic liquid fertilizer containing tylvalosin finished product liquid, comprising the following raw materials: Modified medical stone, humic acid, tylvalosin fermentation broth, pyrethrins, brown sugar, matrine, honey, cinnabar, alum, ethyl garlicin, centipede grass, hymexazol, camphor and water; The ratio of the raw materials is as follows: The weight ratio of the modified medical stone, humic acid, tylvalosin fermentation broth, pyrethrins, brown sugar, matrine, honey, cinnabar, alum, ethyl garlicin, centipede grass, hymexazol, camphor and water is 100 - 1000:4 - 40:20 - 50:0.5 - 5:1.5 - 15:1 - 10:1.2 - 12:1.5 - 15:1.2 - 12:1.1 - 11:1.2 - 12:2 - 20:1.1 - 11:1000 - 3000.

[0020] The beneficial effects of the present invention are as follows: The present invention dilutes the tylvalosin dry powder according to a mass ratio of 1:100 to obtain the tylvalosin finished product liquid; aerobically culture in a liquid medium containing starch, yeast powder, soybean powder, yeast extract and inorganic salts. During the tylvalosin fermentation process, the pH will rise to about 7.5 at the initial stage of fermentation. First, add an appropriate amount of acetic acid to regulate the pH. When the pH drops to 7.3, start to flow in and add a mixture of the substrate tylosin dry powder (800 μg / ml) and the inducer (L-leucine and L-valine). After flowing in this mixture, the pH starts to rise. It is necessary to add an appropriate amount of glucose solution to adjust the pH during the fermentation process to keep it always controlled within 7.00 - 7.30. The fermentation time is controlled within 36 - 110 h to obtain the tylvalosin fermentation broth; Among them, tylosin dry powder: L-leucine: L-valine = 10:2:1 (w:w:w); Put modified picrite, humic acid, tiamulin fermentation broth, pyrethrins, brown sugar, matrine, honey, cinnabar, alum, ethyl garlicin, centipede grass, hymexazol, camphor and water into the fermentation tank according to the above ratio, stir well, keep the temperature within 20 - 30 degrees, stir twice a day, after fermenting for 7 - 10 days, after fermenting for 7 days, an organic liquid fertilizer containing the finished product liquid of tiamulin is obtained; Among them, modified picrite has multiple functions in fertilizers, which are mainly reflected in the following aspects: I. Improve soil properties Adjust the pH value: Modified picrite is a neutral substance that can adjust the pH value of the soil, turning acidic soil into neutral or alkaline soil, thus creating a more suitable soil environment for plant growth.

[0021] Increase air permeability and water retention: The porous structure of modified picrite gives it good air permeability and water retention, which can improve the soil structure, make the soil more loose and breathable, and at the same time improve the water retention capacity of the soil, providing better growth conditions for plant roots.

[0022] II. Provide trace elements Modified picrite contains a large amount of trace elements such as calcium oxide, magnesium oxide, iron oxide, etc. These elements are crucial for plant growth and development. Adding picrite can increase the content of trace elements in the soil, meet the nutritional requirements of plants, and promote the healthy growth of plants.

[0023] III. Promote plant growth and development Improve photosynthesis: Certain components in modified picrite can enhance the photosynthesis of plants, enabling plants to more effectively utilize light energy for growth, thereby increasing the yield and quality of plants.

[0024] Enhance stress resistance: The potassium element in picrite can enhance the stress resistance of plants, enabling plants to still maintain good growth under adverse conditions such as drought and high temperature.

[0025] Enhance disease resistance: The silicon element in picrite can enhance the hardness and stability of the plant cell wall, improve the disease resistance of plants, and prevent the invasion of pests and diseases.

[0026] Tylvalosin, also known as Super tylosin, with the chemical name of 3-O-acetyl-4”-O-isovaleryl tylosin, is an antibiotic specifically for livestock and poultry. Tylvalosin is a new macrolide antibiotic obtained by biotransformation of tylosin by Streptomyces thermotolerans, with acetylation at the 3-position and isovalerylation at the 4”-position. Due to the change in its chemical structure, it has obvious advantages compared with tylosin in terms of antibacterial spectrum, antibacterial activity, drug resistance, chemical stability, etc. The organic liquid fertilizer containing tylvalosin finished liquid of the present invention can effectively improve the growth of crops. Specific Embodiments

[0027] In order to make the technical means, creative features, achieved purposes and functions of the present invention easy to understand, the present invention will be further described below in conjunction with specific embodiments.

[0028] Example 1 An organic liquid fertilizer containing tylvalosin finished liquid and its preparation process according to the embodiment of the present invention include the following steps: Step 1: Dilute tylvalosin dry powder in a ratio of 1:100 by mass to obtain tylvalosin finished liquid; Step 2: Aerobically culture in a liquid medium containing starch, yeast powder, soybean powder, yeast extract and inorganic salts. During the fermentation of tylvalosin, the pH will rise to about 7.5 at the initial stage of fermentation. First, add an appropriate amount of acetic acid to regulate the pH. When the pH drops to 7.3, start to feed a mixture of substrate tylosin dry powder (800 μg / ml) and inducer (L-leucine and L-valine). After feeding this mixture, the pH begins to rise. It is necessary to add an appropriate amount of glucose solution to adjust the pH during the fermentation process to keep it always controlled within 7.00 - 7.30, and the fermentation time is controlled within 36 - 110 h to obtain tylvalosin fermentation broth; Among them, tylosin dry powder : L-leucine : L-valine = 10:2:1 (w:w:w); Step 3: Put modified zeolite, humic acid, tylvalosin fermentation broth, pyrethrin, brown sugar, matrine, honey, cinnabar, alum, ethyl garlicin, centipede grass, hymexazol, camphor and water into the fermentation tank according to the above ratio, stir well, keep the temperature within 20 - 30 degrees, stir twice a day, and after fermenting for 7 - 10 days, after fermenting for 7 days, obtain the organic liquid fertilizer containing tylvalosin finished liquid; Among them, the weight ratio of modified picrite, humic acid, tylosin fermentation broth, pyrethrins, brown sugar, matrine, honey, cinnabar, alum, ethyl garlicin, centipeda minima, hymexazol, camphor and water is 1000:40:50:5:15:10:12:15:12:11:1.12:20:11:3000; Among them, the preparation process of the modified picrite includes the following steps: Take picrite, clean the surface, and perform preliminary crushing after drying; Transfer it to a muffle furnace and calcine it at 300 - 400 °C for 1 - 2 h; Take it out after cooling, add 30 vt% hydrochloric acid solution, and soak it for 3 - 5 h; Wash it with deionized water and dry it in an oven; Put it into a stirred ball mill for grinding for 10 - 25 h. Among them, the grinding medium is steel balls, the mass ratio of balls to materials is 6:1, and the rotational speed of the ball mill is 400 - 450 r / min; Transfer it to a muffle furnace and calcine it at 300 - 400 °C for 60 - 90 min; After taking it out, add 10 wt% cetyltrimethylammonium bromide solution and polyethylene glycol, and stir for 30 - 50 min; Filter and dry; Spread it evenly on the ground electrode in a low-temperature plasma treatment device, and treat it for 10 - 15 min under the conditions of a power frequency of 10 - 15 kHz, a working voltage of 20 kV, and a discharge power of 70 - 80 W; After taking it out, add mannitol, transfer it to an ultrasonic instrument and ultrasonicate for 20 - 30 min. The solid-liquid mass ratio of the picrite powder to mannitol is 1:3; Filter and dry to obtain the modified picrite powder.

[0029] Among them, the solid-liquid mass ratio of the picrite powder to the cetyltrimethylammonium bromide solution is 1:10, and the solid-liquid mass ratio of the picrite powder to polyethylene glycol is 1:8; the particle size of the modified picrite powder ≤ 10 μm.

[0030] Example Two The process for improving the soil air permeability of a compound liquid fertilizer of tylosin finished product liquid according to the embodiment of the present invention includes the following steps: Step 1: Dilute tylosin dry powder according to a mass ratio of 1:300 to obtain tylosin finished product liquid; Step 2: Aerobically cultivate in a liquid medium containing starch, yeast powder, soybean powder, yeast extract, and inorganic salts. During the fermentation process of tylvalosin, the pH will rise to about 7.5 at the initial stage of fermentation. First, add an appropriate amount of acetic acid to regulate the pH. When the pH drops to 7.3, start to feed a mixture of the substrate tylosin dry powder (800 μg / ml) and the inducer (L-leucine and L-valine). After feeding this mixture, the pH starts to rise. It is necessary to add an appropriate amount of glucose solution to adjust the pH during the fermentation process and keep it controlled at 7.00 - 7.30. The fermentation time is controlled at 36 - 110 h to obtain the tylvalosin fermentation broth; Among them, the weight ratio of tylosin dry powder : L-leucine : L-valine = 10 : 2 : 1 (w:w:w); Step 3: Put modified zeolite, humic acid, tylvalosin fermentation broth, pyrethrins, brown sugar, matrine, honey, cinnabar, alum, ethyl garlicin, centipede grass, hymexazol, camphor, and water into the fermentation tank according to the above ratio, stir well. The temperature is within 20 - 30 °C, and stir twice a day. After fermenting for 7 - 10 days, after fermenting for 7 days, obtain the organic liquid fertilizer containing the finished tylvalosin solution; Among them, the weight ratio of modified zeolite, humic acid, tylvalosin fermentation broth, pyrethrins, brown sugar, matrine, honey, cinnabar, alum, ethyl garlicin, centipede grass, hymexazol, camphor, and water is 100 : 4 : 20 : 0.5 : 1.5 : 1 : 1.2 : 1.5 : 1.2 : 1.1 : 1.2 : 2 : 1.1 : 1000; Among them, the preparation process of modified zeolite includes the following steps: Take zeolite, clean the surface, and perform preliminary crushing after drying; Transfer to a muffle furnace and calcine at 300 - 400 °C for 1 - 2 h; Take out after cooling, add 20 vt% hydrochloric acid solution, and soak for 3 - 5 h; Wash with deionized water and dry in an oven; Put into a stirred ball mill and grind for 10 - 25 h. Among them, the grinding medium is steel balls, the ball-to-material mass ratio is 6 : 1, and the rotational speed of the ball mill is 400 - 450 r / min; Transfer to a muffle furnace and calcine at 300 - 400 °C for 60 - 90 min; Take out, add 5 wt% cetyltrimethylammonium bromide solution and polyethylene glycol, and stir for 30 - 50 min; Filter and dry; Evenly spread on the ground electrode in a low-temperature plasma treatment device, and treat for 10 - 15 min under the conditions of a power frequency of 10 - 15 kHz, a working voltage of 20 kV, and a discharge power of 70 - 80 W; After taking it out, mannitol is added, and it is transferred to an ultrasonic instrument for ultrasonic treatment for 20 - 30 min. The solid-liquid mass ratio of the medical stone powder to mannitol is 1:3; Filter and dry to obtain the modified medical stone powder.

[0031] Among them, the solid-liquid mass ratio of the medical stone powder to cetyltrimethylammonium bromide solution is 1:5, and the solid-liquid mass ratio of the medical stone powder to polyethylene glycol is 1:2; the particle size of the modified medical stone powder ≤ 10 μm.

[0032] Example 3 The process for improving the soil air permeability of a composite liquid fertilizer of tylvalosin finished product liquid according to the embodiment of the present invention includes the following steps: Step 1: Dilute the tylvalosin dry powder according to a ratio of 1:500 by mass to obtain the tylvalosin finished product liquid; Step 2: Aerobically culture in a liquid medium containing starch, yeast powder, soybean powder, yeast extract, and inorganic salts. During the tylvalosin fermentation process, the pH will rise to about 7.5 at the initial stage of fermentation. First, add an appropriate amount of acetic acid to regulate the pH. When the pH drops to 7.3, start to flow in and supplement a mixture of the substrate tylosin dry powder (800 μg / ml) and the inducer (L-leucine and L-valine). After flowing in this mixture, the pH starts to rise. It is necessary to supplement an appropriate amount of glucose solution to adjust the pH during the fermentation process to keep it always controlled within 7.00 - 7.30, and the fermentation time is controlled within 36 - 110 h to obtain the tylvalosin fermentation broth; Among them, tylosin dry powder : L-leucine : L-valine = 10:2:1 (w:w:w); Step 3: Put the modified medical stone, humic acid, tylvalosin fermentation broth, pyrethrins, brown sugar, matrine, honey, cinnabar, alum, ethyl garlicin, centipede grass, hymexazol, camphor, and water into a fermentation tank according to the above ratio, stir well. The temperature is within 20 - 30 degrees, stir twice a day. After fermenting for 7 - 10 days, after fermenting for 7 days, obtain the organic liquid fertilizer containing the tylvalosin finished product liquid; Among them, the weight ratio of the modified medical stone, humic acid, tylvalosin fermentation broth, pyrethrins, brown sugar, matrine, honey, cinnabar, alum, ethyl garlicin, centipede grass, hymexazol, camphor, and water is 500:20:30:2:8:6:6:8:7:6:6:10:6:2000; Among them, the preparation process of the modified medical stone includes the following steps: Take the medical stone, clean the surface, and dry it before preliminary crushing; Transfer it to a muffle furnace and roast it at 300 - 400 °C for 1 - 2 h; Take it out after cooling, add 25 vt% hydrochloric acid solution, and soak for 3 - 5 h; Wash with deionized water and dry in an oven; Put it into a stirred ball mill for grinding for 10 - 25 h. Among them, the grinding medium is steel balls, the mass ratio of balls to materials is 6:1, and the rotational speed of the ball mill is 400 - 450 r / min; Transfer it to a muffle furnace and calcine at 300 - 400 °C for 60 - 90 min; After taking it out, add 7 wt% cetyltrimethylammonium bromide solution and polyethylene glycol, and stir for 30 - 50 min; Filter and dry; Evenly spread it on the ground electrode in a low-temperature plasma treatment device, and treat it for 10 - 15 min under the conditions of a power frequency of 10 - 15 kHz, a working voltage of 20 kV, and a discharge power of 70 - 80 W; After taking it out, add mannitol, transfer it to an ultrasonic instrument and ultrasonicate for 20 - 30 min. The solid-liquid mass ratio of the medical stone powder to mannitol is 1:3; Filter and dry to obtain the modified medical stone powder.

[0033] Among them, the solid-liquid mass ratio of the medical stone powder to the cetyltrimethylammonium bromide solution is 1:7, and the solid-liquid mass ratio of the medical stone powder to polyethylene glycol is 1:5; the particle size of the modified medical stone powder ≤ 10 μm.

[0034] Comparative Example 1 Comparative Example 1 adopts the preparation process of an organic liquid fertilizer described above, including the following steps: Put medical stone, humic acid, CM complex bacteria fermentation broth, pyrethrin, brown sugar, matrine, honey, cinnabar, alum, ethyl garlicin, centipede herb, hymexazol, camphor and water into a fermentation tank according to the above ratio, stir well, keep the temperature within 20 - 30 degrees, stir twice a day, and after fermenting for 7 - 10 days, after fermenting for 7 days, obtain an organic liquid fertilizer containing tylvalosin finished liquid; Among them, the weight ratio of medical stone, humic acid, CM complex bacteria fermentation broth, pyrethrin, brown sugar, matrine, honey, cinnabar, alum, ethyl garlicin, centipede herb, hymexazol, camphor and water is 100 - 1000:4 - 40:20 - 50:0.5 - 5:1.5 - 15:1 - 10:1.2 - 12:1.5 - 15:1.2 - 12:1.1 - 11:1.2 - 12:2 - 20:1.1 - 11:1000 - 3000.

[0035] Performance test: For the compound liquid fertilizer of tylvalosin finished product solution prepared in Examples 1-3 and Comparative Example 1, the field experiment took the cultivation of corn as the experimental object. Based on the plant height and root length of corn, the effects of different tylvalosin finished product solution liquid fertilizers on corn production were obtained, and Table 1 was obtained:

[0036] It can be seen from the above table that the compound liquid fertilizer of tylvalosin finished product solution of the present invention can effectively improve the growth of crops.

[0037] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above embodiments. What is described in the above embodiments and the specification only illustrates the principle of the present invention. Without departing from the spirit and scope of the present invention, the present invention will have various changes and improvements, and these changes and improvements all fall within the scope of the present invention claimed. The scope of protection claimed by the present invention is defined by the appended claims and their equivalents.

Claims

1. A process for preparing an organic liquid fertilizer containing tylvalosin finished liquid, characterized in that: The following steps are involved: Step 1: diluting the tyvalosin dry powder at a mass ratio of 1:100-500 to obtain a tyvalosin finished liquid; Step 2: aeration culture is carried out in a liquid culture medium containing starch, yeast powder, soybean powder, yeast paste and inorganic salts. During the fermentation process of tylosin, the pH value will rise to about 7.5 at the initial stage of fermentation. First, an appropriate amount of acetic acid is added to adjust the pH value. When the pH value drops to 7.3, a mixture of substrate tylosin dry powder and an inducer is added. After the mixture is added, the pH value begins to rise. An appropriate amount of glucose solution needs to be added to adjust the pH value of the fermentation process so that the pH value is always controlled at 7.00-7.

30. The fermentation time is controlled at 36-110 hours to obtain a tylosin fermentation liquid. Step 3: Put modified medical stone, humic acid, tyvalosin fermentation liquid, pyrethrin, brown sugar, matrine, honey, cinnabar, alum, ethyl allicin, scutellaria baicalensis, meconium chloride, camphor and water into a fermentation tank in the above proportions, stir well, keep the temperature within 20-30 degrees, stir twice a day, ferment for 7-10 days, and obtain an organic liquid fertilizer containing tyvalosin finished liquid after 7 days of fermentation.

2. A process for preparing an organic liquid fertilizer containing tyvalosin finished liquid according to claim 1, characterized in that: Tylosin dry powder: L-leucine: L-valine = 10:2:1 (w:w:w).

3. A process for preparing an organic liquid fertilizer containing tyvalosin finished liquid according to claim 2, characterized in that: Step 3, the weight ratio of modified medical stone, humic acid, tyvalosin fermentation broth, pyrethrin, brown sugar, matrine, honey, cinnabar, alum, ethyl allicin, herba centipedes, hymexazol, camphor and water is 100-1000:4-40:20-50:0.5-5:1.5-15:1-10:1.2-12:1.5-15:1.2-12:1.1-11:1.2-12:2-20:1.1-11:1000-3000.

4. A process for preparing an organic liquid fertilizer containing tyvalosin finished liquid according to claim 3, characterized in that: In step 3, the preparation process of modified medical stone comprises the following steps: Take medical stone, clean the surface, dry it and then crush it preliminarily; Transfer to a muffle furnace and calcine at 300-400°C for 1-2h; After cooling, take out, add 20-30vt% hydrochloric acid solution, and soak for 3-5h; Wash with deionized water and dry in an oven; Put it into a stirring ball mill for grinding for 10-25 hours, wherein the grinding medium is steel balls, the ball-to-material mass ratio is 6:1, and the ball mill speed is 400-450r / min; Transfer to a muffle furnace and calcine at 300-400°C for 60-90min; After taking out, add 5-10wt% hexadecyltrimethylammonium bromide solution and polyethylene glycol, and stir for 30-50min; Filtering and drying; Evenly spread on the ground electrode in the low-temperature plasma treatment device, and treat for 10-15 minutes under the conditions of power frequency 10-15kHz, working voltage 20kV, and discharge power 70-80W; After taking out, add mannitol and transfer to an ultrasonic instrument for ultrasonication for 20-30 minutes; Filter and dry to obtain modified medical stone powder.

5. The process for preparing an organic liquid fertilizer containing tyvalosin finished liquid according to claim 4, characterized in that: The solid-liquid mass ratio of medical stone powder to hexadecyltrimethylammonium bromide solution is 1:5-10.

6. A process for preparing an organic liquid fertilizer containing tyvalosin finished liquid according to claim 5, characterized in that: The solid-liquid mass ratio of medical stone powder and polyethylene glycol is 1:2-8.

7. The process for preparing an organic liquid fertilizer containing tyvalosin finished liquid according to claim 6, characterized in that: The solid-liquid mass ratio of medical stone powder and polyethylene glycol is 1:2-8.

8. The process for preparing an organic liquid fertilizer containing tyvalosin finished liquid according to claim 7, characterized in that: The particle size of modified medical stone powder is ≤10μm.

9. The process for preparing an organic liquid fertilizer containing tyvalosin finished liquid according to claim 8, characterized in that: The solid-liquid mass ratio of medical stone powder and mannitol is 1:

3.

10. An organic liquid fertilizer containing tylvalosin finished liquid, characterized in that: Including the following ingredients: Modified medical stone, humic acid, tyvalosin fermentation liquid, pyrethrin, brown sugar, matrine, honey, cinnabar, alum, ethyl garlic, basil, hymexazol, camphor and water; The ratio of the raw materials is: The weight ratio of modified medical stone, humic acid, tyvalosin fermentation liquid, pyrethrin, brown sugar, matrine, honey, cinnabar, alum, ethyl allicin, herba centipedes, hymexazol, camphor and water is 100-1000:4-40:20-50:0.5-5:1.5-15:1-10:1.2-12:1.5-15:1.2-12:1.1-11:1.2-12:2-20:1.1-11:1000-3000.

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