Industrial hemp plant hydroponic nutrient solution and application thereof
By combining hydroponic nutrient solution with EM bacteria fermentation broth, compound amino acids and Hogland nutrition solution, the problem of imbalance in trace elements and low active ingredients in the existing hemp hydroponic nutrient solution is solved, and the significant improvement of cannabis roots and stems and leaves growth and the increase of active ingredients content is achieved.
Patent Information
- Application Number
- CN202510497775.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-21
- Publication Date
- 2025-06-13
AI Technical Summary
The existing hemp hydroponic nutrient solution has problems such as imbalance of trace elements, inadequate proportions, poor targeting and low content of active ingredients. It is difficult to effectively promote the growth of cannabis roots and stems and leaves, and increase the content of active ingredients such as CBD.
Provide an industrial hemp plant hydroponic nutrient solution, including EM bacteria fermentation broth, complex amino acids, Hogland nutrient solution, humic acid, chitosan, chlorogenic acid and ferulic acid. Through the combination of these ingredients, the roots and stems and leaves of hemp are promoted, and the content of active ingredients is increased.
This hydroponic nutrient solution can significantly promote the growth of roots and stems and leaves in the early stages of cannabis transplantation, and enhance the content of cannabidiol. Compared with the existing Hogland nutrient solution, its growth-promoting effect is more obvious.
Abstract
Description
Technical Field
[0001] The present invention belongs to the technical field of plant cultivation, and particularly relates to a hydroponic nutrient solution for industrial hemp plants and its application. Background Art
[0002] The fiber of the hemp stem bark is long and tough, and can be used to weave linen, make fishing nets, ropes, etc.; its whole stalk has excellent pulping performance and can be used for papermaking. This "non-tree paper" has the advantage of saving resources; the seeds can be pressed for oil to be used in manufacturing paints, coatings, etc., and the oil residue can also be used as feed, and is widely used in fields such as textiles, papermaking, bio-composites, and medicine.
[0003] Currently, the hydroponic nutrient solution for hemp mostly follows the general Hoagland formula, and there are problems such as unbalanced trace elements, inappropriate ratios, lack of pertinence, and low content of active ingredients. It is hoped to provide a hydroponic nutrient solution for industrial hemp plants to promote the rooting and stem and leaf growth of hemp and increase the content of active ingredients (such as CBD). Summary of the Invention
[0004] The purpose of the present invention is to provide a hydroponic nutrient solution that promotes the rooting of hemp and improves the active ingredients of hemp.
[0005] In order to achieve the above-mentioned invention purpose, the present invention provides the following technical solutions:
[0006] The present invention provides a hydroponic nutrient solution for industrial hemp plants, comprising the following raw materials in parts by weight: 8 - 12 parts of EM bacteria fermentation broth, 1 - 3 parts of compound amino acids, 30 - 40 parts of Hoagland nutrient solution, 4 - 6 parts of humic acid, 0.04 - 0.07 parts of chitosan, 0.01 - 0.03 parts of chlorogenic acid, and 0.01 - 0.03 parts of ferulic acid.
[0007] Preferably, the fermentation substrate of the EM bacteria fermentation broth is straw, soybean meal, poultry manure, and aloe vera juice, and the mass ratio is 6 - 8:3 - 5:1 - 2:1.
[0008] Preferably, the preparation method of the EM bacteria fermentation broth is: mixing straw, soybean meal, manure, and aloe vera juice in proportion, inoculating EM bacteria according to an inoculation amount of 1 - 3%, performing aerobic fermentation at 35 - 40°C for 60 - 90 days, stirring once every 7 - 10 days during the period, adding water 4 - 6 times the total volume of the substrate after fermentation is completed, stirring, oscillating and leaching for 4 - 6 hours, and performing solid-liquid separation to obtain the fermentation broth.
[0009] Preferably, the compound amino acids include cysteine, arginine, aspartic acid, and glutamine. The mass ratio of cysteine, arginine, aspartic acid, and glutamine is 1 - 6:1 - 3:1 - 2:1.
[0010] The present invention also provides the application of the plant hydroponic nutrient solution in cultivating industrial hemp.
[0011] In the present invention, after organic matters such as corn straw, soybean meal, and chicken manure are fermented and decomposed by EM bacteria, various small-molecule soluble substances such as secreted enzymes, peptides, amino acids, soluble sugars, fatty acids, extracellular polysaccharides, and organic nitrogen can be obtained. Hoagland nutrient solution contains rich mineral elements, and the combination of the two can effectively promote the growth of hemp stems and leaves and increase the content of active ingredients in hemp. Chitosan, chlorogenic acid, and ferulic acid cooperate synergistically to further promote root growth and stem and leaf growth. After fermentation, aloe vera juice will produce antibacterial components, which can prevent excessive reproduction of microorganisms when acting together with components such as chlorogenic acid. Chitosan can form a protective film on the roots of plants, and when used together with other components, it can effectively prevent pathogenic microorganisms from invading the roots of plants and ensure the normal growth of plants.
[0012] The plant hydroponic nutrient solution provided by the present invention can promote root growth and stem and leaf growth in the initial stage of hemp transplantation, and can also increase the content of cannabidiol in hemp. Compared with the existing Hoagland nutrient solution, the plant hydroponic nutrient solution provided by the present application has a more obvious growth-promoting effect on hemp. Specific Embodiments
[0013] The formula of the Hoagland nutrient solution in the present invention is: calcium nitrate tetrahydrate 945 mg / L, potassium nitrate 506 mg / L, ammonium nitrate 80 mg / L, potassium dihydrogen phosphate 136 mg / L, magnesium sulfate 493 mg / L, ferrous sulfate heptahydrate 13.9 mg / L, disodium ethylenediaminetetraacetate 18.65 mg / L, potassium iodide 0.83 mg / l, boric acid 6.2 mg / L, manganese sulfate 22.3 mg / L, zinc sulfate 8.6 mg / L, sodium molybdate 0.25 mg / L, copper sulfate 0.025 mg / L, cobalt chloride 0.025 mg / L.
[0014] EM bacteria (20 billion / g) were purchased from Shandong Xinzhuoyuan Biotechnology Co., Ltd., and the product number is 20180620.
[0015] The technical solutions provided by the present invention will be described in detail below in conjunction with embodiments, but they should not be construed as limiting the protection scope of the present invention.
[0016] Example 1
[0017] An industrial hemp plant hydroponic nutrient solution is composed of the following raw materials in parts by weight: 8 parts of EM bacteria fermentation broth, 2 parts of compound amino acids (the mass ratio of cysteine, arginine, aspartic acid, and glutamine is 1:1:1:1), 30 parts of Hoagland nutrient solution, 5 parts of humic acid, 0.05 part of chitosan, 0.02 part of chlorogenic acid, and 0.03 part of ferulic acid.
[0018] The preparation method of the EM bacteria fermentation broth is as follows: Mix corn straw, soybean meal, chicken manure, and aloe vera juice in a mass ratio of 7:5:2:1, adjust the humidity of the substrate to 65%, then inoculate EM bacteria at an inoculation amount of 1%, and perform aerobic fermentation at 37°C for 90 days. Stir once every 10 days during this period. After fermentation is completed, add water 5 times the total volume of the substrate, stir, and perform oscillating extraction for 5 hours, followed by solid-liquid separation to obtain the fermentation broth.
[0019] Example 2
[0020] An industrial hemp plant hydroponic nutrient solution is composed of the following raw materials in parts by weight: 10 parts of EM bacteria fermentation broth, 1 part of compound amino acids (the mass ratio of cysteine, arginine, aspartic acid, and glutamine is 4:3:2:1), 35 parts of Hoagland nutrient solution, 4 parts of humic acid, 0.06 part of chitosan, 0.02 part of chlorogenic acid, and 0.02 part of ferulic acid. The preparation method of the EM bacteria fermentation broth is the same as that in Example 1.
[0021] Example 3
[0022] An industrial hemp plant hydroponic nutrient solution is composed of the following raw materials in parts by weight: 12 parts of EM bacteria fermentation broth, 3 parts of compound amino acids (the mass ratio of cysteine, arginine, aspartic acid, and glutamine is 6:3:2:1), 30 parts of Hoagland nutrient solution, 5 parts of humic acid, 0.04 part of chitosan, 0.03 part of chlorogenic acid, and 0.03 part of ferulic acid. The preparation method of the EM bacteria fermentation broth is the same as that in Example 1.
[0023] Comparative Example 1
[0024] Different from Example 3, in this comparative example, the preparation method of the fermentation broth is as follows: Mix corn straw and chicken manure in a mass ratio of 1:1, then add 1% of EM bacteria, perform aerobic fermentation at 50°C for 30 days, add water 5 times the total volume of the substrate, stir, and perform oscillating extraction for 5 hours, followed by solid-liquid separation to obtain the fermentation broth.
[0025] Comparative Example 2
[0026] Different from Example 3, in this comparative example, no fermentation broth is added.
[0027] Comparative Example 3
[0028] Different from Example 3, in this comparative example, no chitosan is added.
[0029] Comparative Example 4
[0030] Different from Example 3, in this comparative example, salicylic acid is used to replace ferulic acid in equal amount.
[0031] Test Example
[0032] Cannabis sativa L. cv. Yunma 7 was used as the experimental variety. Taking the standard Hoagland nutrient solution formula as the control, and Examples 3 and Comparative Examples 1-4 as the experimental groups, there were a total of 6 groups of treatments, with 5 seedlings in each treatment, and the experiment was repeated 3 times. The seedlings with 2-3 true leaves and uniform growth were removed from the seedling trays, their roots were washed, and they were transferred to 250 mL conical flasks containing different formula nutrient solutions. One seedling was placed in each conical flask and cultured in an artificial climate chamber. The light intensity was set at 2000 lx, the temperature was 25 °C, the humidity was 70%, and the light duration was 16 h / 8 h. The leaves of the seedlings were sprayed with distilled water every day, and the nutrient solution was changed every 7 days. The pH of the nutrient solution used was maintained at 6.0. At 20 days after transplantation, the plant height (from the base of the stem to the growth point), root length, and stem diameter were measured, and the number of new roots was recorded. Harvesting was carried out 145 days after transplantation, and the cannabidiol content was measured (the measurement method was carried out with reference to NY / T 3252.1-2018 "Industrial Hemp Seeds Part 1: Varieties", where the extraction solution was changed to methanol and the mobile phase B was changed to pure water). The results are shown in Table 1 and Table 2:
[0033] Table 1 Effects of Different Nutrient Solutions on Plant Growth
[0034] Group Plant height / cm Root length / cm Stem diameter / mm Number of new roots / root Control group 14.23 6.88 1.58 4.95 Example 3 17.64 12.54 1.83 13.57 Comparative example 1 17.25 10.97 1.76 11.61 Comparative example 2 15.25 8.68 1.64 7.24 Comparative example 3 16.47 9.89 1.71 10.58 Comparative example 4 15.98 9.15 1.68 9.68
[0035] Table 2 Effects of Different Nutrient Solutions on the Content of Active Ingredients
[0036] Group Control group Example 3 Comparative example 1 Comparative example 2 Comparative example 3 Comparative example 4 CBD 1.617％ 2.255％ 2.143％ 1.815％ 1.967％ 1.846％
[0037] As can be seen from the above examples, the hydroponic nutrient solution for plants provided by the present invention can promote the rooting and stem and leaf growth of cannabis in the initial stage of transplantation, and can also increase the cannabidiol content. Compared with Comparative Examples 1-4 and the control group, the plant hydroponic nutrient solution provided by this application has a more obvious growth-promoting effect on cannabis, indicating that the nutrient solution provided by the present invention has more advantages than the existing Hoagland nutrient solution.
[0038] The above are only the preferred embodiments of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and modifications can be made, and these improvements and modifications should also be regarded as the protection scope of the present invention.
Claims
1. A hydroponic nutrient solution for industrial hemp plants, characterized in that: The invention comprises the following raw materials in parts by weight: 8 to 12 parts of EM bacteria fermentation liquid, 1 to 3 parts of compound amino acids, 30 to 40 parts of Hoagland nutrient solution, 4 to 6 parts of humic acid, 0.04 to 0.07 parts of chitosan, 0.01 to 0.03 parts of chlorogenic acid and 0.01 to 0.03 parts of ferulic acid.
2. The plant hydroponic nutrient solution according to claim 1, characterized in that: The fermentation substrates of the EM bacteria fermentation liquid are straw, soybean meal, poultry manure and aloe juice, and the mass ratio is 6-8:3-5:1-2:
1.
3. The plant hydroponic nutrient solution as claimed in claim 2, characterized in that: The preparation method of the EM bacteria fermentation liquid is as follows: straw, soybean meal, feces and aloe juice are mixed in proportion, EM bacteria are inoculated at an inoculation rate of 1-3%, and aerobic fermentation is carried out at 35-40° C. for 60-90 days, during which stirring is performed every 7-10 days, and after the fermentation is completed, water 4-6 times the total volume of the substrate is added, stirred, shaken and extracted for 4-6 hours, and solid-liquid separation is performed to obtain the fermentation liquid.
4. The plant hydroponic nutrient solution according to claim 1, characterized in that: The composition of the complex amino acid includes cysteine, arginine, aspartic acid and glutamine.
5. The plant hydroponic nutrient solution as claimed in claim 4, characterized in that: The mass ratio of cysteine, arginine, aspartic acid and glutamine is 1-6:1-3:1-2:
1.
6. Use of the plant hydroponic nutrient solution according to any one of claims 1 to 5 in cultivating industrial hemp.