Automatic separation device for biological extracellular vesicles

Abstract

The invention provides an automatic separation device for biological extracellular vesicles, and relates to the technical field of biological cell separation, the automatic separation device comprises a fixing frame, a group of storage tanks for storing raw materials and a group of peristaltic pumps are arranged above the fixing frame, a group of separation columns for separation are arranged in the fixing frame, and valves are arranged on the lower surfaces of the group of separation columns; electromagnetic valves for discharging are arranged on the lower surfaces of the group of valves, a group of plungers for extruding air are arranged above the group of separation columns, the group of plungers continuously move and are connected with the group of connection pipes in a sleeving manner through the group of threaded rings, the plungers can extrude samples in the connection pipes, and the samples are separated from the separation columns through the extrusion of the plungers; according to the utility model, a sample can be in full contact with the filter membrane, the filtering speed is extremely low by only relying on gravity for some samples with higher viscosity or containing more impurities, and the pressure difference can be increased by piston extrusion, so that the capability of liquid for overcoming the resistance of the filter membrane is enhanced, the filtering process is obviously accelerated, and the time required by separation is saved.

Description

Technical Field

[0001] The present invention belongs to the technical field of biological cell separation, and more specifically, particularly relates to an automatic separation device for biological extracellular vesicles. Background Art

[0002] As an important carrier for intercellular communication, biological extracellular vesicles exhibit great application potential in the fields of disease diagnosis, treatment, and basic biological research. An automatic separation device for biological extracellular vesicles is a key equipment for accurately and efficiently obtaining extracellular vesicles from complex biological samples. This device aims to utilize advanced technical means to overcome the many drawbacks of traditional manual separation methods and achieve rapid and automated processing of extracellular vesicles.

[0003] During the separation process of biological extracellular vesicles, the following key structures and technologies are commonly used:

[0004] 1. Filter membrane: As one of the core components of separation, it realizes the screening of substances of different sizes through its specific pore size. Filter membranes of different materials and pore sizes, such as polyethersulfone (PES) membranes with pore sizes ranging from dozens of nanometers to micrometers, can be selected according to the size range of extracellular vesicles to retain or permeate specific substances.

[0005] 2. Centrifugation device: Utilizes centrifugal force to make different components in the sample sediment in layers in the centrifuge tube according to differences in density, size, etc. High-speed centrifuges can generate strong centrifugal force to accelerate the separation process and achieve preliminary rough separation in a short time.

[0006] 3. Collection container: Used to collect the separated extracellular vesicles and other components. These containers must have good biocompatibility to prevent affecting the biological activity of extracellular vesicles and usually adopt specially treated plastic or glass materials.

[0007] 4. Control system: Responsible for regulating the entire separation process, including setting the centrifugation speed and time, controlling the pressure and flow rate during the filter membrane separation process, etc., to ensure the stability and repeatability of the separation process.

[0008] Currently, researchers and equipment manufacturers have adopted various technical solutions to achieve efficient separation of extracellular vesicles. Some devices rely solely on high-speed centrifugation and gradually increase the purity of extracellular vesicles by setting different centrifugation parameters and performing multiple centrifugations. Others focus on optimizing the filter membrane separation technology, developing new filter membrane materials and structures, and improving the flux and selectivity of the filter membrane. In addition, some advanced devices attempt to simply combine multiple traditional separation methods, such as centrifugation first and then filtration, in the hope of improving the separation effect.

[0009] However, the above existing embodiments still have the following problems. In simple centrifugal separation, although extracellular vesicles can be initially separated, it is difficult to effectively remove impurities with sizes and densities similar to those of extracellular vesicles, resulting in low separation purity. When relying solely on membrane filtration separation, for samples with high viscosity or a large number of impurities, the filter membrane is prone to clogging, greatly reducing the separation efficiency, and the filtration time is long, which may affect the activity of extracellular vesicles. Moreover, in the existing simple combination of centrifugation and membrane filtration separation, the two technologies operate independently and do not fully utilize their synergistic advantages. The equipment is bulky and the operation is complex. At the same time, during the membrane filtration separation process, there is a lack of an effective stirring mechanism, and problems such as uneven concentration gradient and local precipitation are likely to occur on the surface of the filter membrane, further reducing the separation efficiency. In response to these problems, the present application proposes an innovative solution, designing an automatic separation device for biological extracellular vesicles based on the principle of complementary centrifugal separation and membrane filtration separation and integrated design, and a stirring mechanism is provided in the membrane filtration separation to improve the separation efficiency. This device can effectively improve the separation purity and efficiency of extracellular vesicles, reduce impurity interference, and has a simple operation, providing strong support for the research and application of extracellular vesicles.

[0010] In view of this, research and improvement are carried out on the existing structure and deficiencies to provide an automatic separation device for biological extracellular vesicles, with the expectation of achieving a more practical value. Summary of the Invention

[0011] In order to solve the above technical problems, the present invention provides an automatic separation device for biological extracellular vesicles to solve the above problems.

[0012] The automatic separation device for biological extracellular vesicles includes a fixed frame. Above the fixed frame, there is a set of storage tanks for storing raw materials and a set of peristaltic pumps. Inside the fixed frame, there is a set of separation columns for separation. Valves are provided on the lower surfaces of the set of separation columns, and solenoid valves for discharging materials are provided on the lower surfaces of the set of valves. Above the set of separation columns, there is a set of plungers for squeezing air. A stirring rod for stirring is rotatably connected inside each plunger. Detection probes are provided on the circumferential sides of the set of separation columns. Above the set of plungers, there is a transmission belt, and a set of belt pulleys are movably clamped inside the transmission belt. Threaded rings are threadedly sleeved on the upper ends of the set of separation columns, and a connecting pipe is fixedly connected inside each threaded ring. Two filter meshes are provided below each connecting pipe, and a filter membrane is provided between each pair of filter meshes. Above the transmission belt, there is a second connecting plate. A fixed column is rotatably connected to the upper surface of the second connecting plate, and a first connecting plate is fixedly connected to the upper surface of the fixed column. The set of plungers are respectively movably sleeved with the set of connecting pipes. A protective sleeve is fixedly connected inside the fixed frame, and a second servo motor is fixedly installed inside the protective sleeve.

[0013] Preferably, a fixed disk is rotatably connected to the upper end of the protective sleeve, the output shaft of the second servo motor is fixedly connected to the fixed disk, a group of bolts are fixedly connected to the upper surface of the fixed disk, a limiting block is fixedly connected to the circumferential side of each separating column, and a group of the limiting blocks are respectively movably sleeved on a group of bolts. A cylinder is fixedly connected to the upper surface of the fixed frame, and the piston of the cylinder is fixedly connected to the first connecting plate.

[0014] Preferably, a first servo motor is fixedly installed on the upper surface of the second connecting plate. The output shaft of the first servo motor is fixedly connected to the leftmost pulley in a group of pulleys. A connecting column is fixedly connected to the upper surface of each pulley, and a group of the connecting columns are all fixedly connected to the second connecting plate. A round rod is fixedly connected to the upper surface of each stirring rod, and a group of the round rods are respectively fixedly connected to a group of pulleys.

[0015] Preferably, two limiting rods are fixedly connected to the upper surface of the lower filter screen in each pair of filter screens. A group of the limiting rods are respectively fixedly connected to a group of threaded rings. Two rubber plugs are fixedly connected to the upper surface of the lower filter screen in each pair of filter screens. A pair of the rubber plugs are respectively movably sleeved on the upper filter screen in each pair of filter screens.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] In the present invention, the plunger will extrude the sample in the connecting pipe. Through the extrusion of the plunger, the sample can be more fully contacted with the filter membrane. For some samples with high viscosity or containing more impurities, relying solely on gravity filtration is extremely slow, while piston extrusion can increase the pressure difference, enhance the ability of the liquid to overcome the resistance of the filter membrane, thereby significantly accelerating the filtration process and saving the time required for separation.

[0018] In the present invention, the upper filter screen is moved downward to be sleeved and fixed with two rubber plugs fixed on the lower filter screen, so that the two filter screens are in a tightly fitting state. The two filter screens fix the filter membrane and play a role in buffering and dispersing pressure, reducing the tearing, breakage, etc. of the filter membrane caused by air pressure, thereby prolonging the service life of the filter membrane.

[0019] In the present invention, the stirring action of the stirring rod can make the different components in the sample more fully contact and interact with the surface of the filter membrane, and the stirring can generate local flow and pressure changes inside the sample, cooperate with the extrusion force of the plunger to form a more complex flow field, provide additional power for the sample to pass through the filter membrane, help overcome the resistance of the filter membrane, make the liquid pass through the filter membrane faster, improve the filtration speed, and further accelerate the separation process.

[0020] In the present invention, centrifugal separation utilizes a centrifugal force field to further separate substances with different densities, sizes or shapes under the action of centrifugal force. The overall separation based on centrifugal force complements the principle of membrane filtration separation, enabling more rapid and efficient separation of various components in a mixed system, greatly improving the overall separation efficiency. The integrated operation significantly saves space and time. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 is a schematic diagram of the overall structure of the present invention;

[0022] Figure 2 is a schematic diagram of the structure of the fixing frame of the present invention;

[0023] Figure 3 is a schematic diagram of the structure of the separation column of the present invention;

[0024] Figure 4 is a schematic diagram of the structure of the fixing plate of the present invention;

[0025] Figure 5 is a schematic diagram of the structure of the connecting pipe of the present invention;

[0026] Figure 6 is a schematic diagram of the structure of the filter screen of the present invention;

[0027] Figure 7 is a schematic diagram of the structure of the connecting plate of the present invention;

[0028] Figure 8 is a schematic diagram of the structure of the plunger of the present invention.

[0029] In the figure, the corresponding relationship between the structural names and the drawing reference numerals is as follows: 1, fixing frame; 2, peristaltic pump; 3, storage tank; 4, cylinder; 5, fixing plate; 6, protective sleeve; 7, solenoid valve; 8, valve; 9, separation column; 10, connecting plate 1; 11, fixing column; 12, connecting plate 2; 13, plunger; 14, transmission belt; 15, servo motor 1; 16, bolt; 17, servo motor 2; 18, detection probe; 19, threaded ring; 20, connecting pipe; 21, filter screen; 22, limit block; 23, limit rod; 24, rubber plug; 25, filter membrane; 26, pulley; 27, connecting column; 28, round rod; 29, stirring rod. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0030] The following further describes in detail the embodiments of the present invention in conjunction with the drawings and examples. The following examples are used to illustrate the present invention, but cannot be used to limit the scope of the present invention.

[0031] Please refer to Figure 1 - Figure 8The present invention provides an automatic separation device for biological extracellular vesicles, including a fixed frame 1, a group of storage tanks 3 for storing raw materials and a group of peristaltic pumps 2 are arranged above the fixed frame 1, a group of separation columns 9 for separation are arranged in the fixed frame 1, a group of separation columns 9 are provided with valves 8 on the lower surface, a group of valves 8 are provided with electromagnetic valves 7 for discharging on the lower surface, a group of plungers 13 for squeezing air are arranged above the group of separation columns 9, each plunger 13 is rotatably connected with a stirring rod 29 for stirring, a group of separation columns 9 are provided with detection probes 18 on the annular side, a group of plungers 13 are provided with a transmission belt 14 above the group of plungers 13, a group of pulleys 26 are movably connected in the transmission belt 14, a group of separation columns 9 are threadedly sleeved with a threaded ring 19 at the upper end, each thread A connecting tube 20 is fixedly connected in the ring 19, two filter screens 21 are arranged under each connecting tube 20, and a filter membrane 25 is arranged between each pair of filter screens 21. A connecting plate 2 12 is arranged above the transmission belt 14, and a fixed column 11 is rotatably connected to the upper surface of the connecting plate 2 12, and a connecting plate 10 is fixedly connected to the upper surface of the fixed column 11. A group of plungers 13 are movably connected to a group of connecting tubes 20 respectively, and a protective sleeve 6 is fixedly connected in the fixed frame 1, and a servo motor 2 17 is fixedly installed in the protective sleeve 6. During use, the sample in the storage tank 3 is transported by the peristaltic pump 2, and the sample is transported to the separation column 9 for separation. The sample will first flow through the connecting tube 20 and be initially separated by the filter membrane 25 arranged between the two filter screens 21. The sample The liquid and small molecules in the sample pass through the filter membrane 25, while the exocysts are retained on the upper surface of the filter membrane 25. In order to improve the separation efficiency, the piston of the cylinder 4 is started to drive the fixed connecting plate 10 to move. When the connecting plate 10 moves, it will drive a group of plungers 13 arranged below to move together. A group of plungers 13 continuously moves through a group of threaded rings 19 to be sleeved with a group of connecting tubes 20. During the sleeved process, the plungers 13 will squeeze the sample in the connecting tubes 20. Through the squeezing of the plungers 13, the sample can be more fully contacted with the filter membrane 25. For some samples with high viscosity or containing more impurities, the filtration speed relying solely on gravity is extremely slow, and the piston squeezing can increase the pressure difference, so that the ability of the liquid to overcome the resistance of the filter membrane 25 is enhanced, thereby The filtration process is significantly accelerated and the time required for separation is saved. Filter screens 21 are provided on the upper and lower sides of the filter membrane 25 to fix it, so that the filter membrane 25 will not be damaged due to excessive air pressure. When the filter membrane 25 needs to be replaced, the fixed connecting pipe 20 and the two limit rods 23 are driven to rotate together by rotating the threaded ring 19. At this time, the threaded ring 19 and the limit of the separation column 9 are opened, and the two filter screens 21 can be taken out, and the upper filter screen 21 of the two filter screens 21 is moved upward, and the filter membrane 25 is taken out for replacement. After cleaning, the replaced filter membrane 25 is placed between the two filter screens 21, and the upper filter screen 21 is moved downward so that it is sleeved and fixed with the two rubber plugs 24 fixed on the lower filter screen 21, so that the two filter screens 21 are in a tightly fitted state.The filter membrane 25 is fixed by two filter meshes 21, which play a role in buffering and dispersing pressure, reducing the tearing, damage, etc. of the filter membrane 25 caused by air pressure, thereby prolonging the service life of the filter membrane 25.

[0032] The upper end of the protective sleeve 6 is rotatably connected to the fixed disk 5. The output shaft of the second servo motor 17 is fixedly connected to the fixed disk 5. A set of bolts 16 are fixedly connected to the upper surface of the fixed disk 5. A limiting block 22 is fixedly connected to the circumferential side of each separation column 9. A set of limiting blocks 22 are respectively movably sleeved on a set of bolts 16. A cylinder 4 is fixedly connected to the upper surface of the fixed frame 1. The piston of the cylinder 4 is fixedly connected to the first connecting plate 10. During the extrusion process of the plunger 13, by starting the first servo motor 15, its output shaft will drive the fixed connecting column 27 to rotate. During the rotation of the connecting column 27, the pulley 26 fixed below it will be driven to rotate together. The pulley 26 will drive the transmission belt 14 to rotate. The rotation of the transmission belt 14 will drive the pulleys 26 on the other two sides to rotate together. At this time, the round rod 28 fixed to the lower surface of a set of pulleys 26 is in a rotating state, and the stirring rod 29 fixed to the lower surface of the round rod 28 will stir the sample during rotation. The stirring action of the stirring rod 29 can make the different components in the sample contact and interact more fully with the surface of the filter membrane 25. Moreover, stirring can generate local flow and pressure changes inside the sample, cooperate with the extrusion force of the plunger 13 to form a more complex flow field, provide additional power for the sample to pass through the filter membrane 25, help overcome the resistance of the filter membrane 25, make the liquid pass through the filter membrane 25 faster, improve the filtration speed, and thus accelerate the separation process.

[0033] A servo motor 15 is fixedly installed on the upper surface of the connecting plate two 12. The output shaft of the servo motor 15 is fixedly connected to the leftmost pulley 26 in a set of pulleys 26. A connecting column 27 is fixedly connected to the upper surface of each pulley 26. The set of connecting columns 27 are all fixedly connected to the connecting plate two 12. A round rod 28 is fixedly connected to the upper surface of each stirring rod 29. The set of round rods 28 are respectively fixedly connected to the set of pulleys 26. Two limiting rods 23 are fixedly connected to the upper surface of the lower set of filter meshes 21 in each pair of filter meshes 21. Each pair of limiting rods 23 are respectively fixedly connected to a set of threaded rings 19. Two rubber plugs 24 are fixedly connected to the upper surface of the lower set of filter meshes 21 in each pair of filter meshes 21. Each pair of rubber plugs 24 are respectively movably sleeved on the upper set of filter meshes 21 in each pair of filter meshes 21. After preliminary separation, by starting the servo motor two 17, its output shaft will drive the fixed fixed disk 5 to rotate. The fixed disk 5 is rotatably arranged on the protective sleeve 6. When the fixed disk 5 rotates, a set of separation columns 9 fixed to it by bolts 16 will also rotate accordingly. The plunger 13 sleeved on the upper end of the separation column 9 will also rotate accordingly to ensure the sealing performance of the separation column 9 during the rotation process. After being extruded by the plunger 13, although most substances have been preliminarily separated according to the characteristics of the filter membrane 25, there may still be some substances with similar particle sizes or properties that are not completely separated. Centrifugal separation utilizes the centrifugal force field to further separate substances with different densities, sizes or shapes under the action of centrifugal force. When the raw material needs to be taken out, the valve 8 is opened, and an electrical signal is sent to the solenoid valve 7 through the control system to make it discharge materials. The overall separation effect based on centrifugal force complements the principle of separation by the filter membrane 25, and can separate various components in the mixed system more quickly and effectively, greatly improving the overall separation efficiency. The integrated operation greatly saves space and time.

[0034] Working principle:

[0035] In the first step, during use, the peristaltic pump 2 is used to control the sample in the storage tank 3 to be transported, and the sample is transported to the separation column 9 for separation. The sample will first flow through the connecting tube 20 and be initially separated by the filter membrane 25 set between the two filter screens 21. The liquid and small molecules in the sample pass through the filter membrane 25, while the exocysts are trapped on the upper surface of the filter membrane 25. In order to improve the separation efficiency, by starting the cylinder 4, its piston will drive the fixed connecting plate 10 to move. When the connecting plate 10 moves, it will drive a group of plungers 13 set below to move together. A group of plungers 13 continues to move through a group of threaded rings 19 and a group of connecting tubes 20 for sleeve connection. During the sleeve connection process, the plunger 13 will squeeze the sample in the connecting tube 20. Through the squeezing of the plunger 13, the sample can be more fully contacted with the filter membrane 25. For some samples with high viscosity or containing more impurities, the filtration speed relying solely on gravity is extremely slow, and the piston squeezing can increase the pressure difference so that the liquid overcomes the filter. The resistance of the membrane 25 is enhanced, thereby significantly accelerating the filtration process and saving the time required for separation. The filter membrane 25 is fixed with filter screens 21 on both the upper and lower sides, so that the filter membrane 25 will not be damaged due to excessive air pressure. When the filter membrane 25 needs to be replaced, the fixed connecting pipe 20 and the two limit rods 23 are driven to rotate together by rotating the threaded ring 19. At this time, the threaded ring 19 and the separation column 9 are opened, and the two filter screens 21 can be taken out, and the filter screen 21 located on the upper side of the two filter screens 21 is moved upward, and the filter membrane 25 is taken out for replacement. After cleaning, the replaced filter membrane 25 is placed between the two filter screens 21, and the upper filter screen 21 is moved downward to be sleeved and fixed with the two rubber plugs 24 fixed on the lower filter screen 21, so that the two filter screens 21 are in a tightly fitted state. The filter membrane 25 is fixed by the two filter screens 21 and plays a role in buffering and dispersing pressure, reducing the tearing and damage of the filter membrane 25 caused by air pressure, thereby extending the service life of the filter membrane 25.

[0036] In the second step, during the extrusion process of the plunger 13, the output shaft of the servo motor 15 is started to drive the fixed connecting column 27 to rotate. During the rotation of the connecting column 27, the fixed pulley 26 below it will be driven to rotate together. The pulley 26 will drive the transmission belt 14 to rotate. The rotation of the transmission belt 14 will drive the pulleys 26 on the other two sides to rotate together. At this time, the round rod 28 fixed on the lower surface of a group of pulleys 26 is in a rotating state, and the stirring rod 29 fixed on the lower surface of the round rod 28 will stir the sample when rotating. The stirring action of the stirring rod 29 can make the different components in the sample contact and interact with the surface of the filter membrane 25 more fully, and the stirring can produce local flow and pressure changes inside the sample, which cooperate with the extrusion force of the plunger 13 to form a more complex flow field, providing additional power for the sample to pass through the filter membrane 25, helping to overcome the resistance of the filter membrane 25, allowing the liquid to pass through the filter membrane 25 faster, increasing the filtration speed, and thus accelerating the separation process.

[0037] In the third step, after preliminary separation, by starting the second servo motor 17, its output shaft will drive the fixed fixed disk 5 to rotate. The fixed disk 5 is rotatably arranged on the protective sleeve 6. When the fixed disk 5 rotates, a group of separation columns 9 fixed thereto by bolts 16 will also rotate accordingly. The plunger 13 sleeved on the upper end of the separation column 9 will also rotate accordingly to ensure the sealing performance of the separation column 9 during the rotation process. After being extruded by the plunger 13, although most substances have been preliminarily separated according to the characteristics of the filter membrane 25, there may still be some substances with similar particle sizes or similar properties that have not been completely separated. Centrifugal separation utilizes a centrifugal force field to further separate substances with different densities, sizes, or shapes under the action of centrifugal force. When the raw material needs to be taken out, the valve 8 is opened, and an electrical signal is sent to the solenoid valve 7 through the control system to enable discharging. The separation based on the centrifugal force as a whole complements the principle of separation by the filter membrane 25, and can more quickly and effectively separate various components in the mixed system, greatly improving the overall separation efficiency. The integrated operation greatly saves space and time.

[0038] The embodiments of the present invention are given for purposes of illustration and description, and are not exhaustive or limit the invention to the disclosed form. Many modifications and variations are obvious to those of ordinary skill in the art. The embodiments are chosen and described in order to better illustrate the principles of the invention and its practical application, and to enable those of ordinary skill in the art to understand the invention and design various embodiments with various modifications suitable for a particular purpose.

Claims

1. An automatic separation device for biological extracellular vesicles, comprising a fixing frame (1), characterized in that: A group of material storage tanks (3) for storing raw materials and a group of peristaltic pumps (2) are arranged above the fixed frame (1); a group of separation columns (9) for separation are arranged inside the fixed frame (1); a group of valves (8) are arranged on the lower surface of each of the separation columns (9); a group of solenoid valves (7) for discharging are arranged on the lower surface of each of the valves (8); a group of plungers (13) for squeezing air are arranged above each of the separation columns (9); a stirring rod (29) for stirring is rotatably connected inside each of the plungers (13); and a detection probe (18) is arranged on the annular side surface of each of the separation columns (9); A transmission belt (14) is arranged above a group of the plungers (13), a group of pulleys (26) are movably connected in the transmission belt (14), a threaded ring (19) is threadedly sleeved on the upper end of a group of the separation columns (9), each of the threaded rings (19) is fixedly connected with a connecting pipe (20), two filter screens (21) are arranged below each of the connecting pipes (20), a filter membrane (25) is arranged between each pair of the filter screens (21), a connecting plate 2 (12) is arranged above the transmission belt (14), a fixed column (11) is rotatably connected to the upper surface of the connecting plate 2 (12), a connecting plate 1 (10) is fixedly connected to the upper surface of the fixed column (11), and a group of the plungers (13) are movably sleeved with a group of the connecting pipes (20) respectively.

2. The automatic separation device for biological extracellular vesicles according to claim 1, characterized in that: A protective sleeve (6) is fixedly connected inside the fixing frame (1); Wherein, a servo motor 2 (17) is fixedly installed in the protective sleeve (6).

3. The automatic separation device for biological extracellular vesicles according to claim 2, characterized in that: The upper end of the protective sleeve (6) is rotatably connected to a fixed plate (5); Wherein, the output shaft of the second servo motor (17) is fixedly connected to the fixed disk (5).

4. The automatic separation device for biological extracellular vesicles according to claim 3, characterized in that: A group of bolts (16) are fixedly connected to the upper surface of the fixed plate (5), and a limiting block (22) is fixedly connected to the annular side surface of each separation column (9); Wherein, a group of the limit blocks (22) are respectively movably sleeved with a group of bolts (16).

5. The automatic separation device for biological extracellular vesicles according to claim 1, characterized in that: The upper surface of the fixing frame (1) is fixedly connected with a cylinder (4); Wherein, the piston of the cylinder (4) is fixedly connected to the connecting plate (10).

6. The automatic separation device for biological extracellular vesicles according to claim 1, characterized in that: A servo motor 1 (15) is fixedly mounted on the upper surface of the second connecting plate (12); Wherein, the output shaft of the servo motor 1 (15) is fixedly connected to the leftmost pulley (26) in a group of pulleys (26).

7. The automatic separation device for biological extracellular vesicles according to claim 1, characterized in that: The upper surface of each pulley (26) is fixedly connected with a connecting column (27); Wherein, a group of the connecting columns (27) are all fixedly connected to the second connecting plate (12).

8. The automatic separation device for biological extracellular vesicles according to claim 1, characterized in that: The upper surface of each stirring rod (29) is fixedly connected to a round rod (28); Wherein, a group of round rods (28) are respectively fixedly connected to a group of pulleys (26).

9. The automatic separation device for biological extracellular vesicles according to claim 1, characterized in that: Two limiting rods (23) are fixedly connected to the upper surface of the group of filter screens (21) located at the bottom in each pair of filter screens (21); Wherein, each pair of the limiting rods (23) is respectively fixedly connected to a group of threaded rings (19).

10. The automatic separation device for biological extracellular vesicles according to claim 1, characterized in that: Two rubber plugs (24) are fixedly connected to the upper surface of the group of filter screens (21) located at the bottom in each pair of filter screens (21); Wherein, each pair of rubber stoppers (24) is movably sleeved with a group of filter screens (21) located at the upper side of each pair of filter screens (21).

Images