Kit for screening macromolecular drug targets

Abstract

The invention discloses a macromolecular drug target screening kit, and belongs to the technical field of medical instruments, the macromolecular drug target screening kit comprises a kit body, a plurality of detection modules are installed in the kit body, each detection module comprises a detection plate, the detection plate is provided with a plurality of grooves, detection chips are installed in the grooves, and the detection chips are connected with the detection modules. The height of the highest position of the top of the detection chip is smaller than the height of the opening position of the top of the groove, and a fence is further arranged on the surface of the detection plate and used for defining a closed area to be contained in all the grooves; a liquid inlet pipe is installed at one end of the fence, a liquid outlet pipe is installed at the other end of the fence, valves are installed on the liquid inlet pipe and the liquid outlet pipe, and the height of the end, close to the liquid inlet pipe, of the surface of the detection plate is larger than that of the end, close to the liquid outlet pipe, of the surface of the detection plate, so that the detection plate forms a slope. The detection chip is arranged in the groove, and the top of the detection chip is lower than the groove, so that liquid flowing through the detection plate can be stored in the groove, and the chip can be in full contact with the liquid.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and particularly relates to a kit for screening macromolecular drug targets. Background Art

[0002] Drug target screening is a key step in drug research and development. A drug target refers to the binding site of a drug in the body, and these sites are usually biological macromolecules, such as gene sites, receptors, enzymes, ion channels, nucleic acids, etc. Drugs exert their therapeutic effects by binding to these targets. Drug target screening is a crucial link in the drug research and development process. Through modern technical means such as high-throughput screening and virtual screening, drug molecules that bind to specific targets can be quickly screened out from a large number of candidate compounds. This not only improves the efficiency of drug research and development but also greatly shortens the time from the laboratory to the clinic for new drugs.

[0003] Traditional drug target screening operations are complex. Currently, the main products for target screening are human whole protein chips. Proteins obtained through genetic engineering are listed on the chips. After connecting the chips to a computer and contacting the proteins on the chips with drug reagents, the corresponding drug targets are obtained through high-throughput screening. For the convenience of experiments, all protein chips are integrated into a kit. Since the stability of the protein chips needs to be ensured, the chips are usually protected in a sealed form in the kit. However, this method is not convenient for cleaning after the experiment, and when the chips are in contact with drugs during the experiment, it is also impossible to ensure that the drugs fully contact the chips, thus bringing inconvenience to existing experiments. Summary of the Invention

[0004] The purpose of the present invention is to provide a kit for screening macromolecular drug targets to solve the problems of inconvenient experimental operation and difficult thorough cleaning of the traditional screening kit, which has the advantages that the chips are in more sufficient contact with the liquid medicine and it is convenient to clean after the experiment under the sealed condition of the kit.

[0005] To achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0006] A kit for screening macromolecular drug targets includes a box body. A number of detection modules are installed in the box body. Each detection module includes a detection plate. A number of grooves are opened on the detection plate. Detection chips are installed in the grooves. The height of the highest position at the top of the detection chips is less than the height of the opening position at the top of the grooves. A fence is also provided on the surface of the detection plate for enclosing a closed area including all the grooves;

[0007] One end of the fence is installed with an inlet pipe, and the other end is installed with an outlet pipe. Valves are installed on both the inlet pipe and the outlet pipe. The height of one end of the detection plate close to the inlet pipe is greater than that of the end close to the outlet pipe, so that the detection plate forms a slope.

[0008] Preferably, communication holes are provided between each column of grooves along the direction from the inlet pipe to the outlet pipe. Baffles are installed in the communication holes. The baffles are rotatably installed on the communication holes and are used to control the aperture size of the communication holes blocked by rotating the baffles.

[0009] Preferably, the baffle is installed on a rotating shaft. One end of the rotating shaft passes through the box body and is installed with a gear disk. A moving plate is installed below the gear disk. A rack meshing with the gear disk is installed on the moving plate. The rotation of the baffle is controlled by pushing the moving plate to move.

[0010] Preferably, both the gear disk and the moving plate are installed inside a control box outside the box body. One end of the moving plate passes through the control box and is installed with a control knob.

[0011] Preferably, a slide rail is further provided on the inner wall of the control box, and the moving plate is slidably connected to the slide rail.

[0012] Preferably, the tooth grooves on the gear disk are arranged on the circumference of the gear disk, so that after the rack contacts all the tooth grooves during the movement of the moving plate, the rotation amplitude of the rotating shaft can make the baffle change from not blocking to completely blocking the communication hole.

[0013] Preferably, the vertical cross-section of the communication hole is a fan-shaped ring structure, the baffle is a fan-shaped plate, and the center of the fan-shaped plate coincides with the center of the fan-shaped ring.

[0014] Preferably, the detection module further includes a water tank. The water tank is arranged at the bottom of the detection plate. An electric heating tube is installed inside the water tank. Both the water tank and the detection plate are wrapped by a heat insulation layer.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] 1. By arranging the detection chip in the groove and the top being lower than the groove, the groove can be used to store the liquid flowing through the detection plate, so that the chip can fully contact the liquid. Whether it is the reagent for detection or the cleaning liquid for cleaning, it can fully contact the chip, improving the detection or cleaning effect; and the surface of the detection plate is set as a slope, so that the liquid can quickly flow from the inlet pipe to the outlet pipe, increasing the flow rate of the liquid. By combining the increased flow rate with the liquid storage in the groove, while improving the experimental efficiency, it ensures the full contact between the chip and the liquid.

[0017] 2. A communication hole is also provided between the grooves in the present invention to guide the flow direction of the liquid, enabling the liquid passing through the surface of the detection plate to quickly contact each detection chip. A baffle is provided on the communication hole, facilitating the user to control the aperture size of the communication hole from outside the box to achieve flow control.

[0018] 3. A water tank is also provided below the detection plate of the present invention to control the temperature of the detection plate using the water temperature. Since the specific heat capacity of water is relatively large, the temperature fluctuation is small, and it is relatively stable when controlling the temperature. Therefore, when the detection plate conducts a target screening experiment, a constant temperature environment can be used according to requirements. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the overall structure of the drug target screening kit of the present invention.

[0020] Figure 2 It is a schematic diagram of the distribution structure of the detection module of the present invention in the box body.

[0021] Figure 3 It is a schematic diagram of the structure of the detection module of the present invention.

[0022] Figure 4 It is a schematic diagram of the cross-sectional structure of the detection module of the present invention.

[0023] Figure 5 It is a schematic diagram of the cooperation structure between the communication hole and the baffle of the present invention.

[0024] Figure 6 It is a schematic diagram of the internal structure of the control box of the present invention.

[0025] In the figure: 1. Box body, 2. Liquid inlet pipe, 3. Control box, 4. Control dial, 5. External transmission line, 6. Detection module, 7. Detection plate, 8. Groove, 9. Detection chip, 10. Water tank, 11. Heat insulation layer, 12. Electric heating tube, 13. Fence, 14. Liquid outlet pipe, 15. Rotating shaft, 16. Communication hole, 17. Baffle, 18. Gear disc, 19. Moving plate, 20. Rack, 21. Slide rail. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0026] In order to make the technical means, creative features, achieved purposes and effects of the present invention easy to understand, the present invention will be further described below in conjunction with specific embodiments.

[0027] As shown in Figure 1 and Figure 2As shown, a kit for screening macromolecular drug targets is disclosed, which includes a box body 1. A number of detection modules 6 are installed in the box body 1. The detection modules 6 are used to contact with drugs and output detection data to a computer. An external transmission line 5 is provided on one side of the box body 1 for inserting into the computer input interface to receive the data of the detection module 6. Since there are many types of proteins, in order to adapt to various targets, a plurality of detection modules 6 are provided. Each detection module 6 includes a detection plate 7. A number of grooves 8 are formed in the detection plate 7. A detection chip 9 is installed in the groove 8. The height of the highest position at the top of the detection chip 9 is less than the height of the opening position at the top of the groove 8. A fence 13 is also provided on the surface of the detection plate 7 for enclosing a closed area including all the grooves 8.

[0028] As Figure 3 and Figure 4 shown, the detection chip 9 is lower than the groove 8, so that liquid can be stored in the groove 8. For example, when the detection chip 9 is covered with protein, the detection chip 9 needs to be immersed in the protein diluent for incubation. Since the height of the groove 8 is higher than that of the detection chip 9, the diluent can be stored to provide an incubation environment. After incubation, during the detection experiment, the detection liquid can also be stored in the groove 8 to fully contact with the detection chip 9, reducing experimental errors. The provided fence 13 is used to enclose and store the excess liquid.

[0029] As Figure 4 shown, a liquid inlet pipe 2 is installed at one end of the fence 13, and a liquid outlet pipe 14 is installed at the other end. Valves are installed on both the liquid inlet pipe 2 and the liquid outlet pipe 14. The height of one end of the surface of the detection plate 7 close to the liquid inlet pipe 2 is greater than that of the end close to the liquid outlet pipe 14, making the detection plate 7 form a slope. Different liquids are injected into the kit through the liquid inlet pipe 2, such as the diluent for incubating proteins, the detection liquid required for the experiment, and the cleaning liquid after the experiment. In this way, the entire experimental process can be completed without opening the kit, and the detection chip 9 is also protected. The liquid outlet pipe 14 is used to discharge the liquid. Cooperating with the fence 13, it can control the existence time of the liquid on the surface of the detection plate 7. Valves are provided on both the liquid inlet pipe 2 and the liquid outlet pipe 14 to ensure the tightness of the kit and facilitate the control of the liquid discharge time. Since all liquids enter the kit through the liquid inlet pipe 2 and are discharged through the liquid outlet pipe 14 in this kit, the detection plate 7 is set as an inclined slope, which can facilitate the liquid to quickly pass through the surface area of the detection plate 7 and reach the outlet position of the liquid outlet pipe 14, improving the flow rate of the liquid and thus the experimental efficiency.

[0030] As Figures 4 - 6As shown, communication holes 16 are provided between each row of grooves 8 along the direction from the liquid inlet pipe 2 to the liquid outlet pipe 14. A baffle 17 is installed in the communication hole 16. The baffle 17 is rotatably installed on the communication hole 16 and is used to control the aperture size of the communication hole 16 by rotating the baffle 17. As mentioned above, the grooves 8 are used to store liquid. If the liquid only flows into the grooves 8 from the surface of the detection plate 7, the flow direction is uncontrollable and it cannot be guaranteed that the liquid flows into all the grooves 8 evenly. Only when the liquid volume is large and there is a certain depth within the fence 13 can it be ensured that all the grooves 8 are filled with liquid. However, such a large amount of liquid is required, which is not conducive to saving the experimental cost. Therefore, by setting the communication holes 16, as long as the liquid enters the grooves 8 near the liquid inlet pipe 2, it can flow into all the grooves 8 in this row along the slope, without the need to form a certain liquid depth within the fence 13, thus saving the amount of liquid used. During actual experiments, when we need to clean, the cleaning liquid needs to pass through the surface of the detection chip 9 at a high flow rate in order to wash away the residual liquid medicine on the detection chip 9 and in the grooves 8. During the experiment, we need the liquid to flow slowly through the detection chip 9 so that the liquid medicine has enough time to contact the detection chip 9. Therefore, a baffle 17 is provided in the communication hole 16 to control the flow rate of the liquid to meet the requirements of different experimental processes.

[0031] As Figure 5 and Figure 6 shown, the baffle 17 is installed on the rotating shaft 15. One end of the rotating shaft 15 passes through the box body 1 and is provided with a gear disk 18. A moving plate 19 is installed below the gear disk 18. A rack 20 meshing with the gear disk 18 is installed on the moving plate 19. By pushing the moving plate 19 to move, the rotation of the baffle 17 is controlled. The baffle 17 is set to adjust the throughput of the communication hole 16 by rotation. In order to ensure the stability of the rotation of the baffle 17 and facilitate the control of the angle, the gear transmission method is used to convert the lateral thrust of the moving plate 19 into a rotational force. Therefore, the gear disk 18 is installed on the rotating shaft 15, and the rack 20 is installed on the moving plate 19 and meshes with the gear disk 18.

[0032] To facilitate the user to operate the moving plate 19, both the gear disk 18 and the moving plate 19 are installed inside the control box 3 on the outside of the box body 1. One end of the moving plate 19 passes through the control box 3 and is provided with a control knob 4. The control box 3 can provide a dust-proof and waterproof protection effect for the gear disk 18 and the moving plate 19. The control knob 4 is on the outside of the control box 3, which is convenient for the user to operate. The inner wall of the control box 3 is also provided with a slide rail 21. The moving plate 19 is slidably connected to the slide rail 21, and the slide rail 21 can maintain the stability of the movement of the moving plate 19.

[0033] As Figure 5As shown, the tooth groove part on the gear disk 18 is arranged on the circumference of the gear disk 18. After the rack 20 contacts all the tooth grooves during the movement of the moving plate 19, the rotation amplitude of the rotating shaft 15 can cause the baffle 17 to completely block the communication hole 16 from not being blocked. Since the communication hole 16 is only arranged above the rotating shaft 15, the baffle 17 does not need to rotate around the rotating shaft 15. In order to avoid excessive rotation of the baffle 17, the tooth grooves of the gear disk 18 do not need to be completely arranged around the circumference of the gear disk 18. Only a part is arranged, so that even if the rack 20 has completely contacted all the tooth grooves, the baffle 17 will not rotate excessively, reducing the generation of errors.

[0034] As can be seen from the above, the baffle 17 adjusts the area of blocking the communication hole 16 by rotating. In order to ensure a uniform change in the blocking area Figure 5 As shown, the vertical cross-section of the communication hole 16 is a fan-shaped ring structure, and the baffle 17 is a fan-shaped plate. The center of the fan-shaped plate coincides with the center of the fan-shaped ring. By matching the communication hole 16 with a cross-section of a fan-shaped ring and the fan-shaped baffle 17, when the centers are the same, as the baffle 17 rotates around the center, the area of the fan-shaped ring blocked can be increased or decreased uniformly.

[0035] As Figure 3 As shown, the detection module 6 further includes a water tank 10. The water tank 10 is arranged at the bottom of the detection plate 7. An electric heating tube 12 is installed inside the water tank 10. Both the water tank 10 and the detection plate 7 are wrapped by a heat insulation layer 10. Since the specific heat capacity of water is relatively large and water is used as the heat transfer medium with good stability, the water tank 10 is used to control the temperature of the detection plate 7 to adapt to the temperature of the protein binding reaction, which can ensure the stable progress of the experiment. The electric heating tube 12 is used to heat the water in the water tank 10, and the heat insulation layer 10 is used to reduce the rate of temperature loss.

[0036] For those skilled in the art, it is obvious that the present invention is not limited to the details of the above exemplary embodiments, and can be implemented in other specific forms without departing from the spirit or basic characteristics of the present invention. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-limiting. The scope of the present invention is defined by the appended claims rather than the above description. Therefore, all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be included in the present invention. Any reference signs in the claims should not be regarded as limiting the claims involved.

[0037] In addition, it should be understood that although this specification is described according to embodiments, not every embodiment only contains an independent technical solution. This narrative way of the specification is only for clarity. Those skilled in the art should regard the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A kit for screening macromolecular drug targets, comprising a box body (1), wherein a plurality of detection modules (6) are installed in the box body (1), characterized in that: Each of the detection modules (6) comprises a detection board (7), a plurality of grooves (8) are provided on the detection board (7), a detection chip (9) is installed in the groove (8), the height of the highest position of the top of the detection chip (9) is less than the height of the opening position of the top of the groove (8), and a fence (13) is also provided on the surface of the detection board (7) for enclosing a closed area contained in all the grooves (8); A liquid inlet pipe (2) is installed at one end of the fence (13), and a liquid outlet pipe (14) is installed at the other end. Valves are installed on both the liquid inlet pipe (2) and the liquid outlet pipe (14). The height of the end of the surface of the detection plate (7) close to the liquid inlet pipe (2) is greater than that of the end close to the liquid outlet pipe (14), so that the detection plate (7) forms a slope.

2. A kit for screening macromolecular drug targets according to claim 1, characterized in that: A connecting hole (16) is provided between each row of grooves (8) in the direction from the liquid inlet pipe (2) to the liquid outlet pipe (14), and a baffle (17) is installed in the connecting hole (16). The baffle (17) is rotatably installed on the connecting hole (16) and is used to control the aperture size of the connecting hole (16) by rotating the baffle (17).

3. A kit for screening macromolecular drug targets according to claim 2, characterized in that: The baffle (17) is mounted on the rotating shaft (15). One end of the rotating shaft (15) passes through the box body (1) and is mounted with a gear plate (18). A moving plate (19) is mounted below the gear plate (18). A rack (20) meshing with the gear plate (18) is mounted on the moving plate (19). The baffle (17) is controlled to rotate by pushing the moving plate (19).

4. A kit for screening macromolecular drug targets according to claim 3, characterized in that: The gear plate (18) and the moving plate (19) are both installed inside the control box (3) outside the box body (1), and one end of the moving plate (19) passes through the control box (3) and is installed with a control shift block (4).

5. A kit for screening macromolecular drug targets according to claim 3, characterized in that: The inner wall of the control box (3) is also provided with a slide rail (21), and the movable plate (19) is slidably connected to the slide rail (21).

6. A kit for screening macromolecular drug targets according to claim 1, characterized in that: The tooth grooves on the gear plate (18) are arranged on the circumference of the gear plate (18) so that after the rack (20) contacts all the tooth grooves during the movement of the moving plate (19), the rotation amplitude of the rotating shaft (15) can make the baffle plate (17) completely cover the connecting hole (16) from not covering it.

7. A kit for screening macromolecular drug targets according to claim 1 or 2, characterized in that: The vertical cross section of the communication hole (16) is a fan-shaped ring structure, the baffle (17) is a fan-shaped plate, and the center of the fan-shaped plate coincides with the center of the fan ring.

8. A kit for screening macromolecular drug targets according to claim 1, characterized in that: The detection module (6) further comprises a water tank (10), the water tank (10) being arranged at the bottom of the detection plate (7), an electric heating pipe (12) being installed inside the water tank (10), and both the water tank (10) and the detection plate (7) being wrapped by a heat-insulating layer (10).

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