A culture observation device for paeonia experiment

Abstract

The present application relates to the technical field of peony disease resistance experiment, and particularly relates to a culture observation device for peony disease resistance experiment, which comprises a casing and a culture cover, and further comprises a ventilation and sterilization mechanism, a light adjustment mechanism and an automatic taking mechanism; the culture cover is provided with a flow port at the top for air passing through, and the bottom of the culture cover is provided with a through hole for air discharging; the ventilation and sterilization mechanism is arranged at the top of the casing, and comprises a first fixed disc, a rotating disc, a sealing cover and a first rotating driver; the first fixed disc is arranged at the top of the casing. The vertical laminar flow design of the ventilation and sterilization mechanism, in which air is taken in at the top of the sealing cover and discharged through the bottom of the first fixed disc, can reduce airflow disturbance and mechanical damage to peonies, and air only enters through the air vents arranged at the top of the sealing cover, thereby avoiding cross contamination caused by disorderly flow.

Description

Technical Field

[0001] This invention relates to the field of experimental technology for peony disease resistance, specifically to a culture and observation device for peony disease resistance experiments. Background Technology

[0002] In the field of peony disease resistance research, cultivation and observation devices are indispensable key equipment. However, existing peony cultivation and observation devices still have some shortcomings in design and function, especially in terms of ventilation and ease of operation. These problems limit experimental efficiency and data accuracy to some extent.

[0003] Specifically, traditional peony cultivation and observation devices often lack efficient ventilation systems. As a plant highly sensitive to its growing environment, the disease resistance of peonies is closely related to the ventilation conditions of that environment. Poor ventilation not only affects the normal growth and development of peonies but may also exacerbate the occurrence and spread of diseases. However, existing devices often overlook this point, resulting in the inability to provide suitable ventilation conditions for peonies during experiments, thus affecting the accuracy of experimental results.

[0004] Chinese Patent Publication No. CN117016259B discloses a cultivation and observation device based on vegetable disease resistance experiments. The device includes a cultivation and observation box, a vegetable cultivation tray, a root and stem observation mechanism, an auxiliary lifting mechanism, and a pathogen spraying mechanism. This invention provides a cultivation and observation device for vegetable disease resistance experiments. When it is necessary to observe the condition of the rear side of the vegetable root and stem after pathogen inoculation, an image of the rear root and stem area of ​​the vegetable is captured by a camera located between the support ring and the connecting ring, and the captured image is displayed on a monitor. Furthermore, if the vegetable tilts after pathogen inoculation, the distance between the vegetable and three inclined reference plates on the upper surface of the connecting ring can be observed. If the distance between the vegetable and one of the inclined reference plates deviates from the distance of the other two inclined reference plates, it indicates that the vegetable is tilting, thus facilitating the experimenter's judgment on whether the vegetable has tilted after pathogen inoculation.

[0005] However, in practical use, the aforementioned structure relies on natural convection or simple mechanical fans for ventilation, resulting in uncontrollable airflow direction. This can easily lead to the spread of pathogens with the airflow, causing cross-contamination between samples. Furthermore, the lack of filtration and sterilization modules during the ventilation process allows external microorganisms to easily invade, and internal pathogens may contaminate the laboratory environment, thereby affecting the accuracy of the experiments. Summary of the Invention

[0006] To address the aforementioned issues, a cultivation and observation device for peony disease resistance experiments is provided. Through a ventilation and sterilization mechanism, the problems of mechanical damage to peonies caused by airflow disturbance and cross-contamination caused by disordered airflow are solved.

[0007] To address the problems of existing technologies, this invention provides a cultivation and observation device for peony disease resistance experiments, comprising a casing and a cultivation hood. The device further includes a ventilation and sterilization mechanism, a light adjustment mechanism, and an automatic handling mechanism. The top of the cultivation hood has an air inlet for air passage, and the bottom of the hood has a through-hole for air exhaust. The ventilation and sterilization mechanism is located on the top of the casing and includes a first fixed plate, a rotating plate, a sealing cover, and a first rotation driver. The first fixed plate is located on the top of the casing, and its top has multiple first exhaust ports for gas exhaust, which correspond to the positions of the through-holes at the bottom of the cultivation hood. The rotating plate is rotatably mounted on the top of the casing and positioned above the first fixed plate. The rotating disk has multiple second outlets corresponding to the outlet positions on its top, and multiple placement slots for placing culture hoods on its top. A sealing cover is slidably mounted on the top of the rotating disk, and multiple ventilation openings corresponding to the second outlet positions are located on its top. A first rotary drive is located on the top of the housing and below the first fixed disk, and its output end is fixedly connected to the rotating disk. A light adjustment mechanism is located on the top of the housing and above the sealing cover, and is used to heat the culture hoods inside the sealing cover. An automatic retrieval mechanism is located on the top of the housing and beside the light adjustment mechanism, and is used to lift the sealing cover and detach it from the outside of the rotating disk.

[0008] Preferably, the ventilation and sterilization mechanism further includes a filter box, a multi-stage filter plate, and an air inlet pipe; the filter box is located on top of the sealing cover, and an opening is provided on the top of the filter box; the multi-stage filter plate is slidably located on top of the filter box; multiple air inlets are located outside the filter box, and each of the multiple air inlets is connected to a ventilation opening on the top of the sealing cover.

[0009] Preferably, the ventilation and sterilization mechanism further includes a negative pressure generator, a sterilization box, and ultraviolet lamps; a discharge chamber is provided on the top of the first fixed plate; the sterilization box is disposed inside the first fixed plate and connected to the negative pressure generator, and a processing chamber for gas recovery is provided inside the sterilization box; there are multiple negative pressure generators, which are respectively disposed inside the first fixed plate, and the multiple negative pressure generators are respectively connected to the discharge port provided on the top of the first fixed plate; there are multiple ultraviolet lamps, which are respectively disposed inside the sterilization box.

[0010] Preferably, the ventilation and sterilization mechanism further includes a drainage pipe and an exhaust fan; the drainage pipe has a pair and is respectively disposed outside the first fixed plate, and the drainage pipe is connected to the sterilization box; the exhaust fan has a pair and is respectively disposed on the top of the casing and is connected to the drainage pipe.

[0011] Preferably, the illumination adjustment mechanism includes a first linear driver, a lead screw, a sliding plate, a limiting block, and a displacement illumination block; the first linear driver is disposed on the top of the housing and located beside the exhaust fan; the lead screw is rotatably disposed on the top of the housing and connected to the output end of the first linear driver; the sliding plate is slidably disposed on the top of the housing and threadedly connected to the lead screw; there are multiple limiting blocks, each disposed on the top of the housing, and each limiting block has a groove on its top; there are multiple displacement illumination blocks, each slidably disposed on the top of the limiting blocks.

[0012] Preferably, the illumination adjustment mechanism further includes illumination tubes and connecting rods; there are multiple illumination tubes respectively arranged on one side of the displacement illumination block, and the illumination direction of the multiple illumination tubes is all facing the sealing cover; the connecting rod is rotatably arranged on the top of the displacement illumination block, one end of the connecting rod is rotatably connected to the displacement illumination block, and the other end of the connecting rod is rotatably connected to the sliding plate.

[0013] Preferably, the light adjustment mechanism further includes a limiting rod; the limiting rod has a pair and is respectively disposed on the top of the housing, and the sliding plate and the limiting rod are fitted with a clearance.

[0014] Preferably, the automatic picking mechanism includes a second linear actuator, a threaded rod, mounting blocks, and a displacement block; the second linear actuator is disposed on the top of the housing and located beside the rotating disk; the threaded rod is rotatably disposed on the top of the housing and connected to the output end of the second linear actuator; there is a pair of mounting blocks, each disposed on the top of the housing, and both mounting blocks are located beside the second linear actuator; the displacement block is slidably disposed between the pair of mounting blocks and threadedly connected to the threaded rod.

[0015] Preferably, the automatic retrieval mechanism further includes a telescopic cylinder and an ejector block; the telescopic cylinder has a pair and is respectively disposed on the top of the displacement block; the ejector block is disposed at the output end of the telescopic cylinder and is T-shaped.

[0016] Preferably, the automatic retrieval mechanism further includes a connecting plate; the connecting plate is disposed outside the sealing cover and is fixedly connected to the ejector block.

[0017] The advantages of this invention compared to the prior art are:

[0018] 1. This invention, by setting up a ventilation and sterilization mechanism, adopts a vertical laminar flow design with air intake at the top of the sealed cover and exhaust through the bottom of the first fixed plate. This reduces airflow disturbance and prevents mechanical damage to the peony. Furthermore, air enters only through the ventilation opening at the top of the sealed cover, avoiding cross-contamination caused by disordered flow.

[0019] 2. This invention, through the setting of a light adjustment mechanism, addresses the low light intensity required during the peony seedling stage. At this stage, multiple movable light blocks are positioned relatively far apart, and the light temperature of the lamps is adjusted via a temperature controller to achieve low-light operation. However, when the peony matures into a bulbous plant, its growth characteristics become wider and taller, requiring higher light intensity. At this stage, multiple movable light blocks are positioned relatively close together, and the light temperature of the lamps is again adjusted via a temperature controller to achieve high-light operation. This simulates natural light to adapt to the different growth stages of the peony.

[0020] 3. This invention enables researchers to quickly remove the culture cover by setting up an automatic retrieval mechanism. Attached Figure Description

[0021] Figure 1 This is a first-view three-dimensional structural diagram of a peony disease resistance experiment cultivation and observation device according to the present invention.

[0022] Figure 2 This is a partial cross-sectional three-dimensional structural diagram of a peony disease resistance experiment cultivation and observation device according to the present invention.

[0023] Figure 3 This is a top view of a cultivation and observation device for peony disease resistance experiments according to the present invention.

[0024] Figure 4 This is a three-dimensional structural diagram of the ventilation and sterilization mechanism of a cultivation and observation device for peony disease resistance experiments according to the present invention.

[0025] Figure 5 This is a partial cross-sectional three-dimensional structural diagram of the ventilation and sterilization mechanism of a cultivation and observation device for peony disease resistance experiments according to the present invention.

[0026] Figure 6 yes Figure 5 Enlarged structural diagram at point A in the middle.

[0027] Figure 7 yes Figure 5 Enlarged structural diagram at point B in the middle.

[0028] Figure 8 This is a three-dimensional structural diagram of the sterilization box of a peony disease resistance experiment cultivation and observation device according to the present invention.

[0029] Figure 9 This is a three-dimensional structural diagram of the light adjustment mechanism of a cultivation and observation device for peony disease resistance experiment according to the present invention.

[0030] Figure 10 This is a three-dimensional structural diagram of a cultivation and observation device for peony disease resistance experiment in which the displacement light block is relatively far away from the object.

[0031] Figure 11This is a three-dimensional structural diagram of the automatic retrieval mechanism of a peony disease resistance experiment cultivation and observation device in the retrieval state.

[0032] The diagram is labeled as follows: 1. Housing; 2. Culture hood; 21. Through-hole; 3. Ventilation and sterilization mechanism; 31. First fixed plate; 311. First outlet; 32. Rotary plate; 322. Second outlet; 33. Sealing cover; 331. Ventilation port; 34. First rotary actuator; 35. Filter box; 36. Multi-stage filter plate; 37. Air inlet pipe; 38. Negative pressure generator; 39. Sterilization box; 391. Ultraviolet lamp; 392. 393. Drainage pipe; 4. Exhaust fan; 5. Lighting adjustment mechanism; 41. First linear actuator; 42. Lead screw; 43. Sliding plate; 44. Limiting block; 45. Displacement lighting block; 46. Lighting tube; 47. Connecting rod; 48. Limiting rod; 5. Automatic retrieval mechanism; 51. Second linear actuator; 52. Threaded rod; 53. Mounting block; 54. Displacement block; 55. Telescopic cylinder; 56. Ejection block; 57. Connecting plate. Detailed Implementation

[0033] To further understand the features, technical means, and specific objectives and functions achieved by the present invention, the present invention will be described in further detail below with reference to the accompanying drawings and specific embodiments.

[0034] See Figures 1-4As shown, a peony disease resistance experiment cultivation and observation device includes a housing 1 and a cultivation hood 2. The cultivation and observation device also includes a ventilation and sterilization mechanism 3, a light adjustment mechanism 4, and an automatic handling mechanism 5. The top of the cultivation hood 2 has an air inlet for air passage, and the bottom of the cultivation hood 2 has a through-hole 21 for air exhaust. The ventilation and sterilization mechanism 3 is located on the top of the housing 1 and includes a first fixed plate 31, a rotating plate 32, a sealing cover 33, and a first rotation driver 34. The first fixed plate 31 is located on the top of the housing 1, and the top of the first fixed plate 31 has multiple first exhaust outlets 311 for gas exhaust, which correspond to the positions of the through-hole 21 at the bottom of the cultivation hood 2. The rotating plate 32 is rotatably mounted on the top of the housing 1 and located above the first fixed plate 31, and the top of the rotating plate 32 is... The rotating disk 32 has multiple second outlets 322 corresponding to the outlet positions, and multiple placement slots for placing the culture hood 2 are provided on the top of the rotating disk 32. The sealing cover 33 is slidably disposed on the top of the rotating disk 32, and multiple ventilation openings 331 corresponding to the positions of the second outlets 322 are provided on the top of the sealing cover 33. The first rotary drive 34 is disposed on the top of the housing 1 and located below the first fixed disk 31, and the output end of the first rotary drive 34 is fixedly connected to the rotating disk 32. The light adjustment mechanism 4 is disposed on the top of the housing 1 and located above the sealing cover 33, and the light adjustment mechanism 4 is used to provide light heating for the culture hood 2 inside the sealing cover 33. The automatic pick-up mechanism 5 is disposed on the top of the housing 1 and located beside the light adjustment mechanism 4, and the automatic pick-up mechanism 5 is used to lift the sealing cover 33 and remove it from the outside of the rotating disk 32.

[0035] The first fixed plate 31, the rotating plate 32, and the sealing cover 33 are all made of transparent glass, which will not affect the observation of the peonies placed inside the culture hood 2. First, the peonies to be cultured are placed inside the culture hood 2. Then, the sealing cover 33 is lifted by the automatic pick-up mechanism 5, maintaining a certain distance between the culture hood 2 and the rotating plate 32. Next, the peonies inside the culture hood 2 are slid into the placement slot opened on the top plate of the rotating plate 32. At this point, the installation of the culture hood 2 and the peonies is complete. The first fixed plate 31 is fixedly connected to the top of the housing 1, and the sealing cover 33 will not rotate with the rotating plate 32 under the drive of the first rotary drive 34. In the initial state, the second outlet 322 opened on the top of the rotating plate 32 does not correspond to the position of the first outlet 311 opened on the top of the first fixed plate 31. (Instruction manual attached) Figure 5The diagram illustrates the corresponding positions of the through hole, the second outlet 322, and the vent 331. When ventilation of the culture chamber 2 inside the sealed cover 33 is required, the first rotary driver 34 is activated, causing the rotating disk 32 to rotate. Airflow is achieved when the second outlet 322 at the top of the rotating disk 32 aligns perfectly with the vent 331 at the top of the sealed cover 33. This vertical laminar flow design, with air intake at the top of the sealed cover 33 and exhaust through the bottom of the first fixed disk 31, aims to reduce mechanical damage to the peony caused by airflow disturbance. It simulates airflow dynamics in the natural environment while ensuring that experimental isolation requirements are met. Furthermore, air enters only through the vent 331 at the top of the sealed cover 33, effectively avoiding cross-contamination caused by disordered flow.

[0036] See Figures 4-7 As shown, the ventilation and sterilization mechanism 3 also includes a filter box 35, a multi-stage filter plate 36, and an air inlet pipe 37; the filter box 35 is located on the top of the sealing cover 33, and an opening is provided on the top of the filter box 35; the multi-stage filter plate 36 is slidably located on the top of the filter box 35; the air inlet pipe 37 is located outside the filter box 35 and there are multiple air inlet pipes 37, and the multiple air inlet pipes 37 are respectively connected to the ventilation openings 331 opened on the top of the sealing cover 33.

[0037] When the second exhaust is opened at the top of the rotating disk 32 and corresponds to the position of the vent 331 opened at the top of the sealing cover 33, air can flow into the interior of the sealing cover 33 through the multi-stage filter plate 36 and the air inlet pipe 37. The multi-stage filter plate 36 is preferably made of HEPA filter material. The multi-stage filter plate 36 can refresh the air and block the invasion of external pathogens or the spread of internal pathogens.

[0038] See Figures 5-7 As shown, the ventilation and sterilization mechanism 3 also includes a negative pressure generator 38, a sterilization box 39, and an ultraviolet lamp 391; the top of the first fixed plate 31 has an exhaust chamber; the sterilization box 39 is disposed inside the first fixed plate 31 and is connected to the negative pressure generator 38, and the inside of the sterilization box 39 has a processing chamber for gas recovery; there are multiple negative pressure generators 38, which are respectively disposed inside the first fixed plate 31, and the multiple negative pressure generators 38 are respectively connected to the exhaust port opened at the top of the first fixed plate 31; there are multiple ultraviolet lamps 391, which are respectively disposed inside the sterilization box 39.

[0039] When the second exhaust port on the top of the rotating disc 32 corresponds to the vent 331 on the top of the sealing cover 33, the negative pressure generator 38 located inside the first fixed disc 31 is activated. The air pressure difference drives external air through the filter box 35 and the multi-stage filter plate 36, forming a directional airflow path from the outside to the inside of the sealing cover 33 and then out from the first exhaust port 311, effectively avoiding cross-infection. When air intake is not needed, simply turn off the negative pressure generator 38 to stop air intake. When exhaust is needed, the rotating disc 32 rotates until the second exhaust port on the top of the rotating disc 32 no longer coincides with the vent 331 on the top of the sealing cover 33. Then, the negative pressure generator 38 continues to start and exhausts the air inside the culture cover 2 into the sterilization box 39. Then, the ultraviolet lamp 391 sterilizes the harmful components in the air. By adjusting the air intake of the negative pressure generator 38, the amount of air entering can be indirectly affected. The negative pressure generator 38 is preferably a laboratory-grade model with precise pressure control, low noise, and corrosion resistance, such as the KNF model negative pressure generator 38 or the GAST model negative pressure generator 38.

[0040] See Figure 7 and Figure 8 As shown, the ventilation and sterilization mechanism 3 also includes a drainage pipe 392 and an exhaust fan 393; the drainage pipe 392 has a pair and is respectively disposed outside the first fixed plate 31, and the drainage pipe 392 is connected to the sterilization box 39; the exhaust fan 393 has a pair and is respectively disposed on the top of the housing 1 and is connected to the drainage pipe 392.

[0041] After the harmful gas has stayed inside the sterilization box 39 for a period of time, the exhaust fan 393 starts and discharges the sterilized air through the drainage pipe 392, thus achieving sterilization of the air after ventilation and harmless discharge.

[0042] See Figure 9 and Figure 10 As shown, the illumination adjustment mechanism 4 includes a first linear actuator 41, a lead screw 42, a sliding plate 43, a limiting block 44, and a displacement illumination block 45. The first linear actuator 41 is disposed on the top of the housing 1 and located beside the exhaust fan 393. The lead screw 42 is rotatably disposed on the top of the housing 1 and connected to the output end of the first linear actuator 41. The sliding plate 43 is slidably disposed on the top of the housing 1 and threadedly connected to the lead screw 42. There are multiple limiting blocks 44, which are respectively disposed on the top of the housing 1, and each of the multiple limiting blocks 44 has a sliding groove on its top. There are multiple displacement illumination blocks 45, which are respectively slidably disposed on the top of the limiting blocks 44.

[0043] When the sealing cover 33 needs to be illuminated, the first linear driver 41 is started and drives the lead screw 42 to rotate. When the lead screw 42 rotates, it can drive the sliding plate 43 to rise. When the sliding plate 43 falls, it can drive multiple displacement illumination blocks 45 to move relatively close to each other along the limiting block 44.

[0044] See Figure 9 and Figure 10 As shown, the illumination adjustment mechanism 4 also includes illumination tubes 46 and connecting rods 47; there are multiple illumination tubes 46, which are respectively arranged on one side of the displacement illumination block 45, and the illumination direction of the multiple illumination tubes 46 is all facing the sealing cover 33; the connecting rod 47 is rotatably arranged on the top of the displacement illumination block 45, one end of the connecting rod 47 is rotatably connected to the displacement illumination block 45, and the other end of the connecting rod 47 is rotatably connected to the sliding plate 43.

[0045] A temperature controller and a temperature sensor are installed on the top of the displacement lighting block 45. The temperature controller is used to adjust the illumination temperature of the illumination tube 46, and the temperature sensor is used to display and detect the illumination temperature of the illumination tube 46. The temperature sensor and the temperature controller are electrically connected. When the sliding plate 43 rises, it can pull multiple rotatably connected displacement lighting blocks 45 and illumination tubes 46 towards the sealing cover 33 via the connecting rod 47. Then, the illumination tubes 46 are activated and illuminate the cultivation cover 2 inside the sealing cover 33. During the peony seedling stage, low light intensity is required. At this time, the multiple displacement lighting blocks 45 are relatively far apart, and the illumination temperature of the illumination tubes 46 is adjusted by the temperature controller to achieve low light operation. When the peony reaches the pearl stage, its growth characteristics are wide spacing and high height, requiring high light intensity. At this time, the multiple displacement lighting blocks 45 will be relatively close, and the illumination temperature of the illumination tubes 46 is adjusted by the temperature controller to achieve high light operation. This simulates natural light to adapt to different growth stages of the peony.

[0046] See Figure 9 and Figure 10 As shown, the illumination adjustment mechanism 4 also includes a limiting rod 48; the limiting rod 48 has a pair and is respectively disposed on the top of the housing 1, and the sliding plate 43 and the limiting rod 48 are fitted with a clearance.

[0047] When the sliding plate 43 moves, it can move stably along the limit rod 48, which improves the stability of the sliding plate 43.

[0048] See Figure 11As shown, the automatic picking mechanism 5 includes a second linear actuator 51, a threaded rod 52, a mounting block 53, and a displacement block 54. The second linear actuator 51 is disposed on the top of the housing 1 and located beside the rotating disk 32. The threaded rod 52 is rotatably disposed on the top of the housing 1 and connected to the output end of the second linear actuator 51. There is a pair of mounting blocks 53, which are respectively disposed on the top of the housing 1, and both of the mounting blocks 53 are located beside the second linear actuator 51. The displacement block 54 is slidably disposed between the pair of mounting blocks 53 and threadedly connected to the threaded rod 52.

[0049] When it is necessary to remove the peony inside the culture hood 2, the second linear actuator 51 is first started and drives the threaded rod 52 to rotate. When the threaded rod 52 rotates, it can drive the displacement block 54 to move.

[0050] See Figure 11 As shown, the automatic picking mechanism 5 also includes a telescopic cylinder 55 and an ejector block 56; the telescopic cylinder 55 has a pair and is respectively disposed on the top of the displacement block 54; the ejector block 56 is disposed at the output end of the telescopic cylinder 55 and the ejector block 56 is T-shaped.

[0051] When the displacement block 54 moves, it can drive the mounting block 53 and the telescopic cylinder 55 to move synchronously. At this time, the telescopic cylinder 55 will lift the ejector block 56.

[0052] See Figure 11 As shown, the automatic retrieval mechanism 5 also includes a connecting plate 57; the connecting plate 57 is disposed outside the sealing cover 33, and the connecting plate 57 is fixedly connected to the ejector block 56.

[0053] When the ejector block 56 is lifted, it can drive the connecting plate 57 to rise synchronously. When the connecting plate 57 rises, it can drive the sealing cover 33 to rise synchronously until the sealing cover 33 is separated from the outside of the rotating disk 32. Then the displacement block 54 moves away from the rotating disk 32 along the mounting block 53. At this time, the experimenter can take out the culture cover 2.

[0054] Working principle: Initially, the second outlet 322 at the top of the rotating disk 32 does not correspond to the first outlet 311 at the top of the first fixed disk 31. When ventilation of the culture hood 2 inside the sealing cover 33 is required, the first rotary driver 34 is activated to rotate the rotating disk 32. When the second outlet 322 at the top of the rotating disk 32 is fully aligned with the vent 331 at the top of the sealing cover 33, air can enter through the top of the sealing cover 33 and exit through the bottom of the first fixed disk 31, achieving a vertical laminar flow design. This aims to reduce mechanical damage to the peony caused by airflow disturbance while meeting experimental isolation requirements. Air enters through the vent 331 at the top of the sealing cover 33, passes through the multi-stage filter plate 36 (preferably HEPA filter material) and the air inlet pipe 37, and flows into the interior of the sealing cover 33, effectively avoiding cross-contamination caused by disordered flow. At this time, the negative pressure generator 38 inside the first fixed plate 31 starts, and the air pressure difference drives the outside air to enter through the filter box 35 and the multi-stage filter plate 36, forming a directional airflow path. The air enters the sealed cover 33 from the outside and then exits from the first outlet 311, effectively avoiding cross-contamination. When air intake is not needed, the negative pressure generator 38 can be turned off to stop air intake. When exhaust is needed, the rotating plate 32 continues to rotate until the second outlet 322 and the vent 331 no longer coincide. The negative pressure generator 38 then starts again and exhausts the air inside the culture hood 2 into the sterilization box 39, where the ultraviolet lamp 391 sterilizes harmful components in the air. The amount of air entering can be indirectly controlled by adjusting the air intake of the negative pressure generator 38. The negative pressure generator 38 is preferably a laboratory-grade KNF negative pressure generator 38 model with precise pressure control, low noise, and corrosion resistance. After sterilization, the exhaust fan 393 starts and exhausts the sterilized air through the drainage pipe 392, achieving harmless emission. When the sealing cover 33 needs to be illuminated, the first linear actuator 41 is activated to rotate the lead screw 42, which in turn drives the sliding plate 43 to rise or fall. The sliding plate 43 pulls multiple displacement illumination blocks 45 and illumination lamps 46 towards or away from the sealing cover 33 via the connecting rod 47. The top of the displacement illumination block 45 is equipped with a temperature controller and a temperature sensor to adjust and display the illumination temperature of the illumination lamps 46. At different growth stages of the peony, by adjusting the relative position and illumination temperature of the displacement illumination blocks 45, natural light is simulated to meet the growth needs of the peony. The sliding plate 43 moves stably along the limiting rod 48, improving movement stability. When it is necessary to remove the peony inside the cultivation cover 2, the second linear actuator 51 is activated to rotate the threaded rod 52, which in turn drives the displacement block 54 to move. The displacement block 54 drives the mounting block 53 and the telescopic cylinder 55 to move synchronously. The telescopic cylinder 55 lifts the ejector block 56, which in turn drives the connecting plate 57 and the sealing cover 33 to rise synchronously. Until the sealing cover 33 is detached from the outside of the rotating disk 32, the displacement block 54 moves away from the rotating disk 32 along the mounting block 53. At this time, the experimenter can remove the culture cover 2.

[0055] The above embodiments only illustrate one or more implementations of the present invention, and their descriptions are relatively specific and detailed, but they should not be construed as limiting the scope of the present invention. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these all fall within the protection scope of the present invention. Therefore, the protection scope of the present invention should be determined by the appended claims.

Claims

1. A culture and observation device for peony disease resistance experiment, comprising a casing (1) and a culture cover (2), characterized in that, The cultivation and observation device also includes a ventilation and sterilization mechanism (3), a light adjustment mechanism (4), and an automatic retrieval mechanism (5); The top of the culture hood (2) is provided with an outlet for air to pass through, and the bottom of the culture hood (2) is provided with a through-hole (21) for air to be discharged. The ventilation and sterilization mechanism (3) is located on the top of the housing (1). The ventilation and sterilization mechanism (3) includes a first fixed plate (31), a rotating plate (32), a sealing cover (33), and a first rotating drive (34). The first fixed plate (31) is set on the top of the casing (1), and the top of the first fixed plate (31) is provided with multiple first outlets (311) for gas discharge. The multiple first outlets (311) correspond to the positions of the through-holes (21) opened at the bottom of the culture hood (2). The rotating disk (32) is rotatably mounted on the top of the housing (1) and located above the first fixed disk (31). The top of the rotating disk (32) is provided with multiple second outlets (322) corresponding to the positions of the first outlets. The top of the rotating disk (32) is provided with multiple placement slots for placing the culture hood (2). The sealing cover (33) is slidably disposed on the top of the rotating disk (32), and the top of the sealing cover (33) is provided with multiple ventilation openings (331) corresponding to the position of the second outlet (322). The first rotary driver (34) is located on the top of the housing (1) and below the first fixed disk (31), and the output end of the first rotary driver (34) is fixedly connected to the rotary disk (32); The light adjustment mechanism (4) is set on the top of the housing (1) and above the sealing cover (33). The light adjustment mechanism (4) is used to heat the culture container (2) inside the sealing cover (33) by light. The automatic retrieval mechanism (5) is located on the top of the housing (1) and next to the light adjustment mechanism (4), and the automatic retrieval mechanism (5) is used to lift the sealing cover (33) and disengage it from the outside of the rotating disk (32). The ventilation and sterilization mechanism (3) also includes a filter box (35), a multi-stage filter plate (36), and an air inlet pipe (37); the filter box (35) is located on the top of the sealing cover (33), and an opening is provided on the top of the filter box (35); the multi-stage filter plate (36) is slidably located on the top of the filter box (35); the air inlet pipe (37) is located outside the filter box (35) and there are multiple pipes, and the multiple air inlet pipes (37) are respectively connected to the ventilation opening (331) opened on the top of the sealing cover (33); The ventilation and sterilization mechanism (3) also includes a negative pressure generator (38), a sterilization box (39), and an ultraviolet lamp (391); the top of the first fixed plate (31) is provided with an exhaust chamber; the sterilization box (39) is located inside the first fixed plate (31) and is connected to the negative pressure generator (38), and the inside of the sterilization box (39) is provided with a processing chamber for gas recovery; there are multiple negative pressure generators (38) and they are respectively located inside the first fixed plate (31), and the multiple negative pressure generators (38) are respectively connected to the first exhaust port opened at the top of the first fixed plate (31); there are multiple ultraviolet lamps (391) and they are respectively located inside the sterilization box (39); The ventilation and sterilization mechanism (3) also includes a drainage pipe (392) and an exhaust fan (393); the drainage pipe (392) has a pair and is respectively located outside the first fixed plate (31), and the drainage pipe (392) is connected to the sterilization box (39); the exhaust fan (393) has a pair and is respectively located on the top of the housing (1) and is connected to the drainage pipe (392).

2. The culture and observation device for peony disease resistance experiment according to claim 1, characterized in that, The illumination adjustment mechanism (4) includes a first linear actuator (41), a lead screw (42), a sliding plate (43), a limiting block (44), and a displacement illumination block (45); the first linear actuator (41) is located on the top of the housing (1) and next to the exhaust fan (393); the lead screw (42) is rotatably located on the top of the housing (1) and connected to the output end of the first linear actuator (41); the sliding plate (43) is slidably located on the top of the housing (1) and threadedly connected to the lead screw (42); there are multiple limiting blocks (44) and they are respectively located on the top of the housing (1), and each of the multiple limiting blocks (44) has a sliding groove on its top; there are multiple displacement illumination blocks (45) and they are respectively slidably located on the top of the limiting blocks (44).

3. The culture and observation device for peony disease resistance experiment according to claim 2, characterized in that, The lighting adjustment mechanism (4) also includes a lighting tube (46) and a connecting rod (47); there are multiple lighting tubes (46) respectively arranged on one side of the displacement lighting block (45), and the lighting direction of the multiple lighting tubes (46) is facing the sealing cover (33); the connecting rod (47) is rotatably arranged on the top of the displacement lighting block (45), one end of the connecting rod (47) is rotatably connected to the displacement lighting block (45), and the other end of the connecting rod (47) is rotatably connected to the sliding plate (43).

4. The culture and observation device for peony disease resistance experiment according to claim 2, characterized in that, The illumination adjustment mechanism (4) also includes a limiting rod (48); the limiting rod (48) has a pair and is respectively disposed on the top of the housing (1), and the sliding plate (43) and the limiting rod (48) are fitted with a clearance.

5. The culture and observation device for peony disease resistance experiment according to claim 1, characterized in that, The automatic picking mechanism (5) includes a second linear actuator (51), a threaded rod (52), a mounting block (53), and a displacement block (54); the second linear actuator (51) is located on the top of the housing (1) and beside the rotating disk (32); the threaded rod (52) is rotatably located on the top of the housing (1) and connected to the output end of the second linear actuator (51); there is a pair of mounting blocks (53) and they are respectively located on the top of the housing (1), and both of the mounting blocks (53) are located beside the second linear actuator (51); the displacement block (54) is slidably located between the pair of mounting blocks (53) and threadedly connected to the threaded rod (52).

6. The culture and observation device for peony disease resistance experiment according to claim 5, characterized in that, The automatic retrieval mechanism (5) also includes a telescopic cylinder (55) and an ejector block (56); the telescopic cylinder (55) has a pair and is respectively disposed on the top of the displacement block (54); the ejector block (56) is disposed at the output end of the telescopic cylinder (55) and the ejector block (56) is T-shaped.

7. The culture and observation device for peony disease resistance experiment according to claim 6, characterized in that, The automatic retrieval mechanism (5) also includes a connecting plate (57); the connecting plate (57) is located outside the sealing cover (33), and the connecting plate (57) is fixedly connected to the ejector block (56).

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