Eye drops for treating eye diseases and preparation method thereof

Abstract

The invention discloses eye drops for treating eye diseases and a preparation method of the eye drops, and relates to the technical field of traditional Chinese medicine preparations. The traditional Chinese medicine extracting solution is an extracting solution of mother-of-pearl, flos eriocauli, herba equiseti hiemalis, bat dung, flos buddlejae, semen celosiae and borneol. The invention aims to provide the eye drops for treating the eye diseases and the preparation method thereof, the eye drops have good prevention and treatment effects on various eye diseases, and the problems that in the prior art, many impurity residues exist, effective substances are difficult to permeate, and the use effect is poor are solved.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine preparations, and in particular, to an eye drop for treating eye diseases and a preparation method thereof. Background Art

[0002] The existing eye drops generally have the following defects: containing preservatives such as benzalkonium chloride, which damage corneal epithelial cells after long-term use; chemically synthesized drugs (such as antibiotics) are prone to drug resistance; in the existing patent (CN101797331A, a traditional Chinese medicine patch for preventing and treating ophthalmic diseases), the prepared eye medicine liquid is immersed in sterile non-woven fabric to make an eye patch for use, but it has a lot of impurity residues, may cause ocular surface irritation after long-term use, and the effective substances are difficult to penetrate during the use of the patch, resulting in poor use effects. Summary of the Invention

[0003] The purpose of the present invention is to provide an eye drop for treating eye diseases and a preparation method thereof, which have good preventive and therapeutic effects on various eye diseases, and solve the problems of a lot of impurity residues, difficult penetration of effective substances, and poor use effects in the prior art.

[0004] The embodiments of the present invention are achieved through the following technical solutions:

[0005] An eye drop for treating eye diseases, comprising a traditional Chinese medicine extract and injection water; the traditional Chinese medicine extract is an extract of nacre, Eriocaulon buergerianum, Equisetum hiemale, vespertilio feces, Buddleja officinalis, Celosia argentea, and borneol.

[0006] The traditional Chinese medicine extract comprises the following components in parts by mass:

[0007] 40 - 60 parts of nacre, 24 - 36 parts of Eriocaulon buergerianum, 24 - 36 parts of Equisetum hiemale, 24 - 36 parts of vespertilio feces, 24 - 36 parts of Buddleja officinalis, 24 - 36 parts of Celosia argentea, and 32 - 48 parts of borneol;

[0008] The injection water is 960 - 1440 parts.

[0009] Further, the traditional Chinese medicine extract is composed of the following raw materials in parts by mass:

[0010] 50 parts of nacre, 30 parts of Eriocaulon buergerianum, 30 parts of Equisetum hiemale, 30 parts of vespertilio feces, 30 parts of Buddleja officinalis, 30 parts of Celosia argentea, and 40 parts of borneol;

[0011] The injection water is 1200 parts.

[0012] A preparation method of an eye drop for treating eye diseases, comprising the following steps:

[0013] S1. Wash the prepared raw materials, and then soak Eriocaulon buergerianum, Equisetum hiemale, vespertilio feces, Buddleja officinalis, and Celosia argentea in the injection water;

[0014] S2. Boil the soaked medicinal material mixture in step S1 and conduct preliminary filtration to obtain a preliminary filtrate;

[0015] S3. Add injection water to the medicinal materials after filtration in step S2, boil them, and conduct secondary filtration to obtain a secondary filtrate;

[0016] S4. Combine the preliminary filtrate and the secondary filtrate, and then conduct tertiary filtration to obtain a tertiary filtrate;

[0017] S5. Crush nacre to obtain nacre powder, and add it to the tertiary filtrate and stir to obtain a mixture;

[0018] S6. Let the mixture obtained in step S5 stand still, and then conduct filtration;

[0019] S7. Repeat step S6 and gradually increase the filtration mesh number to obtain a quaternary filtrate;

[0020] S8. Dissolve borneol in an ethanol solution, add it to the quaternary filtrate and let it stand still;

[0021] S9. Extract the clear part in the middle of the liquid obtained in step S8, and fill it into an eye drop bottle and seal it;

[0022] Further, the masses of Eriocaulon buergerianum, Equisetum hiemale, Vespertilio superans, Buddleja officinalis, and Celosia argentea are 3 g respectively; the mass of nacre is 5 g; the mass of borneol is 4 g; the injection water is 120 ml.

[0023] Further, the preliminary filtration uses 80 meshes; the secondary filtration uses 80 meshes; the tertiary filtration uses 120 meshes.

[0024] Further, in step S7, when gradually increasing the filtration mesh number, 120 meshes, 180 meshes, and 200 meshes are used respectively.

[0025] Further, the standing time is 24 h.

[0026] The technical solution of the embodiment of the present invention has at least the following advantages and beneficial effects:

[0027] An eye drop for treating eye diseases and its preparation method according to the present invention provide a traditional Chinese medicine compound preparation without preservatives and chemical additives through a formula of pure traditional Chinese medicine. Its medicinal properties are mild and it is not easy to produce drug resistance. The prescription therein is derived from the theory of clearing the liver and improving eyesight in "Yinhai Jingwei". Mother-of-pearl: Sweet, cold, non-toxic in nature, belonging to the heart and liver meridians, with the effects of calming the mind and relieving uneasiness, removing nebula and improving eyesight; Vespertilio feces: Cold in nature, pungent in taste, slightly toxic, belonging to the liver meridian, with the effects of clearing the liver and improving eyesight, dispersing stasis and reducing accumulation, swelling and pain of the eyes, nourishing essence and blood, and treating internal and external nebulae; Buddleja officinalis: Slightly cold in nature, belonging to the liver meridian, with the effects of clearing the liver and improving eyesight, dispersing stasis and reducing accumulation, swelling and pain of the eyes, and removing internal and external nebulae; Eriocaulon buergerianum: Sweet, flat, slightly toxic in nature, belonging to the heart, liver, lung and stomach meridians, with the effects of dispersing wind-heat and removing nebula and improving eyesight; Equisetum hiemale: Sweet, flat in nature, belonging to the liver and lung meridians, with the effects of dispersing wind-heat, tearing in the wind, and removing nebula and improving eyesight; Celosia argentea: Bitter, slightly cold in nature, belonging to the liver meridian, with the effects of removing liver fire, clearing heat and drying dampness, swelling and pain of the eyes, nebula in the eyes, blurred vision, and removing nebula and improving eyesight; Borneol: Pungent, bitter, slightly cold in nature, belonging to the heart, spleen and lung meridians, with the effects of resuscitating the consciousness, clearing heat and relieving pain, and promoting tissue regeneration and relieving swelling.

[0028] After modern pharmacodynamic screening and optimization, calcined mother-of-pearl is rich in calcium carbonate and amino acids, and it has been experimentally confirmed that it can increase the migration speed of corneal epithelial cells; Vespertilio feces contains vitamin A derivatives, and its retinol content detected by HPLC reaches 1.2 mg / g, which can promote the regeneration of retinal photoreceptor cells; the silicate component in Equisetum hiemale can physically cover the damaged cornea and reduce external stimulation; the flavonoids in Buddleja officinalis can increase the activity of corneal antioxidant enzymes; the polysaccharide of Eriocaulon buergerianum is used to regulate the osmotic pressure of tears, which is superior to artificial tears; through the synergistic effect of multiple components, anti-inflammatory, repair, nutrition and other effects are achieved; and the combination of borneol + alkaloids of Celosia argentea forms a broad-spectrum antibacterial combination to form a natural anti-corrosion system; the core advantage of the present invention is to combine the "overall regulation" characteristics of traditional Chinese medicine with modern preparation technology, completely avoiding the risk of chemical additives while ensuring the curative effect, and providing a safer long-term medication option for the treatment of ocular surface diseases. Detailed implementation manners

[0029] To make the objectives, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are some, but not all, of the embodiments of the present invention.

[0030] All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the scope of protection of the present invention.

[0031] Example 1

[0032] An eye drop for treating eye diseases is composed of the following raw materials: the masses of Eriocaulon buergerianum, Equisetum hiemale, Vespertilio superans, Buddleja officinalis, and Celosia argentea are each 2.4 g; the mass of nacre is 4 g; the mass of borneol is 3.2 g; and the injection water is 96 ml.

[0033] A preparation method of an eye drop for treating eye diseases includes the following steps:

[0034] S1. Wash the prepared raw materials, and then soak Eriocaulon buergerianum, Equisetum hiemale, Vespertilio superans, Buddleja officinalis, and Celosia argentea in 48 ml of injection water.

[0035] S2. Boil the soaked medicinal material mixture in step S1, and preliminarily filter it through a 80-mesh sieve to obtain a preliminary filtrate.

[0036] S3. Add another 48 ml of injection water to the medicinal materials after filtration in step S2, boil them, and perform secondary filtration through a 80-mesh sieve to obtain a secondary filtrate.

[0037] S4. Combine the preliminary filtrate and the secondary filtrate, and then perform tertiary filtration through a 120-mesh sieve to obtain a tertiary filtrate.

[0038] S5. Crush nacre to obtain pearl powder, and add it to the tertiary filtrate and stir to obtain a mixed solution.

[0039] S6. Let the mixed solution obtained in step S5 stand for 24 h, and then filter it.

[0040] S7. Repeat step S6, and gradually increase the filtration mesh number in sequence according to 120-mesh, 180-mesh, and 200-mesh to obtain a quaternary filtrate.

[0041] S8. Dissolve borneol in an ethanol solution, add it to the quaternary filtrate, and let it stand for 24 h.

[0042] S9. Extract the clear part in the middle of the liquid obtained in step S8, and fill it into an eye drop bottle and seal it.

[0043] Example 2

[0044] An eye drop for treating eye diseases is composed of the following raw materials: the masses of Eriocaulon buergerianum, Equisetum hiemale, Vespertilio superans, Buddleja officinalis, and Celosia argentea are each 3 g; the mass of nacre is 5 g; the mass of borneol is 4 g; and the injection water is 120 ml.

[0045] A preparation method of an eye drop for treating eye diseases includes the following steps:

[0046] S1. Wash the prepared raw materials, and then soak Eriocaulon buergerianum, Equisetum hiemale, Vespertilio superans, Buddleja officinalis, and Celosia argentea in 60 ml of injection water.

[0047] S2. Boil the soaked medicinal material mixture in step S1, and conduct a preliminary filtration with 80 meshes to obtain a preliminary filtrate;

[0048] S3. Add 60 ml of injection water to the medicinal materials after filtration in step S2, boil them, and conduct a secondary filtration with 80 meshes to obtain a secondary filtrate;

[0049] S4. Combine the preliminary filtrate and the secondary filtrate, and then conduct a tertiary filtration with 120 meshes to obtain a tertiary filtrate;

[0050] S5. Crush mother-of-pearl to obtain mother-of-pearl powder, and add it to the tertiary filtrate and stir to obtain a mixed solution;

[0051] S6. Let the mixed solution obtained in step S5 stand for 24 h, and then conduct filtration;

[0052] S7. Repeat step S6, and gradually increase the filtration mesh number, increasing successively according to 120 meshes, 180 meshes, and 200 meshes to obtain a fourth filtrate;

[0053] S8. Dissolve borneol in an ethanol solution, add it to the fourth filtrate and let it stand for 24 h;

[0054] S9. Extract the clear part in the middle of the liquid obtained in step S8, and fill it into an eye drop bottle and seal it.

[0055] Example 3

[0056] An eye drop for treating eye diseases, which is composed of the following raw materials: the masses of Eriocaulon buergerianum, Equisetum hiemale, Vespertilio superans, Buddleja officinalis, and Celosia argentea are 3.6 g respectively; the mass of mother-of-pearl is 6 g; the mass of borneol is 4.8 g; the injection water is 144 ml.

[0057] A preparation method of an eye drop for treating eye diseases, comprising the following steps:

[0058] S1. Wash the prepared raw materials, and then add Eriocaulon buergerianum, Equisetum hiemale, Vespertilio superans, Buddleja officinalis, and Celosia argentea to 72 ml of injection water for soaking;

[0059] S2. Boil the soaked medicinal material mixture in step S1, and conduct a preliminary filtration with 80 meshes to obtain a preliminary filtrate;

[0060] S3. Add 72 ml of injection water to the medicinal materials after filtration in step S2, boil them, and conduct a secondary filtration with 80 meshes to obtain a secondary filtrate;

[0061] S4. Combine the preliminary filtrate and the secondary filtrate, and then conduct a tertiary filtration with 120 meshes to obtain a tertiary filtrate;

[0062] S5. Crush nacre to obtain pearl powder, and add it to the filtrate for three times and stir to obtain a mixed solution;

[0063] S6. Let the mixed solution obtained in step S5 stand for 24 h, and then filter it;

[0064] S7. Repeat step S6, and gradually increase the filtration mesh number, increasing in sequence of 120 mesh, 180 mesh and 200 mesh to obtain the filtrate for four times;

[0065] S8. Dissolve borneol in an ethanol solution, add it to the filtrate for four times and let it stand for 24 h;

[0066] S9. Extract the clear part in the middle of the liquid obtained in step S8, and fill it into an eye drop bottle and seal it.

[0067] Experimental modeling

[0068] Experimental animals are experimental rabbits; the environment is controlled at a temperature of 22±2°C, a humidity of 55±5%, and a 12 h light cycle.

[0069] Model 1: Bacterial keratitis

[0070] Modeling method: After anesthesia, use a corneal trephine to make an epithelial defect with a diameter of 3 mm, and inoculate 10 μL of a suspension of Staphylococcus aureus (ATCC 25923) at 5×10 6 CFU / mL;

[0071] Grouping:

[0072] Blank control group: Instill normal saline into the eyes;

[0073] General control group: 0.5% levofloxacin eye drops;

[0074] Experimental group 1: Eye drops of Example 1;

[0075] Experimental group 2: Eye drops of Example 2;

[0076] Experimental group 3: Eye drops of Example 3.

[0077] Model 2: Dry eye

[0078] Modeling method: Instill 0.2% benzalkonium chloride into the eyes, 3 times a day for 14 consecutive days;

[0079] Grouping:

[0080] Blank control group: Untreated healthy rabbits;

[0081] General control group: Instill normal saline into the eyes after modeling;

[0082] Experimental group 1: Eye drops of Example 1;

[0083] Experimental group 2: Eye drops of Example 2;

[0084] Experimental group 3: Eye drops of Example 3.

[0085] Model 3: Video terminal visual fatigue

[0086] Modeling method: Customize an LED screen (blue light peak 450nm, brightness 800cd / m 2 ), and force the subjects to gaze at it for 6 hours every day for 7 consecutive days;

[0087] Grouping:

[0088] Blank control group: Normal light without intervention;

[0089] General control group: Instill physiological saline after modeling;

[0090] Experimental group 1: Eye drops of Example 1;

[0091] Experimental group 2: Eye drops of Example 2;

[0092] Experimental group 3: Eye drops of Example 3.

[0093] Administration method

[0094] Frequency: 4 times a day (8:00 / 12:00 / 16:00 / 20:00)

[0095] Dose: 1 drop each time (35 μL / eye)

[0096] Course of treatment:

[0097] Keratitis model: Treat for 7 days;

[0098] Dry eye model: Treat for 14 days;

[0099] Visual fatigue model: Administer prophylactic drugs during the treatment period.

[0100] Experimental results

[0101] The eye drops described in Example 1, Example 2, and Example 3 can all effectively inhibit keratitis in Model 1, and the tear film of the experimental rabbits in Model 2 is stable, and the eyes of the experimental rabbits in Model 3 are in good condition;

[0102] The eye drops prepared in other embodiments of the present invention can all effectively treat various eye diseases;

[0103] Moreover, no adverse reactions such as corneal edema / neovascularization occurred in the three groups of examples.

[0104] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. An eye drop for treating eye diseases, characterized in that: The invention comprises a Chinese medicine extract and water for injection; the Chinese medicine extract is an extract of mother-of-pearl, herba schizonepetae, herba equisetifoliae, herba pubescens, herba buddlejae, herba cyperi and borneol.

2. An eye drop for treating eye diseases, characterized in that: The Chinese medicine extract comprises the following components in parts by weight: 40-60 parts of mother-of-pearl, 24-36 parts of Herba Equisetifoliae, 24-36 parts of Psoralea corylifoliae, 24-36 parts of Buddleja buds, 24-36 parts of Atractylodes macrocephala, and 32-48 parts of Borneol; The water for injection is 960-1440 parts.

3. An eye drop for treating eye diseases, characterized in that: The Chinese medicine extract is composed of the following raw materials in parts by weight: 50 parts of mother of pearl, 30 parts of Herba Codonopsis pilosulae, 30 parts of Equisetum arvense, 30 parts of Psoralea corylifolia, 30 parts of Buddleja flos, 30 parts of Atractylodes macrocephala, 40 parts of Borneolum; The water for injection is 1200 parts.

4. A method for preparing the eye drops for treating eye diseases according to any one of claims 1 to 3, characterized in that: The following steps are involved: S1. Wash the prepared raw materials, and then soak the herb, horsetail, night-shining sand, buddleja, and green amaranth in water for injection; S2. The mixed solution of medicinal materials soaked in step S1 is boiled and initially filtered to obtain a preliminary filtrate; S3. Add water for injection again to the filtered medicinal material in step S2 and boil it, and perform secondary filtration to obtain a secondary filtrate; S4. The preliminary filtrate and the secondary filtrate are combined, and then filtered three times to obtain three filtrates; S5. The mother-of-pearl was crushed to obtain pearl powder, and the mixture was added to the filtrate three times and stirred to obtain a mixture; S6. The mixed solution obtained in step S5 is allowed to stand and then filtered; S7 repeat step S6, and gradually increase the filtration mesh number to obtain four filtrates; S8. Dissolve borneol in ethanol solution, add it to the filtrate four times and let it stand; S9. Extract the clear portion of the liquid obtained in step S8 and fill it into an eye drop bottle and seal it; 5. The method for preparing eye drops for treating eye diseases according to claim 4, characterized in that: The masses of the Herba Corydalis, Equisetum arvense, Psoralea corylifolia, Buddleja buddleja and Atractylodes macrocephala are 3 g respectively; the mass of the mother of pearl is 5 g; the mass of the borneol is 4 g; and the water for injection is 120 ml.

6. The method for preparing eye drops for treating eye diseases according to claim 4, characterized in that: The primary filtration uses 80 mesh; the secondary filtration uses 80 mesh; and the tertiary filtration uses 120 mesh.

7. The method for preparing eye drops for treating eye diseases according to claim 4, characterized in that: In step S7, when gradually increasing the filtration mesh number, 120 mesh, 180 mesh and 200 mesh are used respectively.

8. The method for preparing eye drops for treating eye diseases according to claim 4, characterized in that: The standing time is 24 hours.