A composition for preventing and alleviating acute drunkenness

Abstract

The present application relates to a kind of compositions for preventing and relieving acute drunkenness, which is prepared from the following raw materials by weight: 30 parts of Gegen, 10 parts of Chengma, 10 parts of Zhupencao, 15 parts of Zexie, 15 parts of Baishu, 30 parts of Fuling, 15 parts of Xianhecao, 15 parts of Gaoliangjiang and 30 parts of Baimaogen.The composition provided by the present application is a multi-target and multi-path protective process for treating acute alcoholism, involving anti-inflammatory, anti-allergic, promoting protein synthesis, immunosuppression, anti-shock, regulating blood lipids and cholesterol, participating in alcohol decomposition and metabolic pathways; affecting transcription factors such as SP1, ESR1, RELA (P65), NF-κb1 and STAT3, which can not only prevent acute drunkenness, but also reduce the harm of alcohol metabolism after drunkenness, including hyperlipidemia, dementia, hypercholesterolemia, fatty liver, cognitive impairment, cardiovascular and cerebrovascular diseases, etc. In addition, it can also protect the cardiovascular, nervous and skeletal systems after drunkenness, regulate immunity and enhance the immune capacity after drunkenness.

Description

Technical Field

[0001] This invention relates to the field of medical technology, and specifically to a composition for preventing and alleviating acute intoxication. Background Technology

[0002] Acute alcohol intoxication (AAI), commonly known as drunkenness, has become one of the most common types of poisoning in the emergency department.

[0003] Modern medicine primarily treats alcohol-induced alcoholic beverage (AAI) with symptomatic and supportive care, including fluid replacement, maintaining airway patency, preserving circulatory function, and maintaining water, electrolyte, and acid-base balance. In addition, interventions are often made with alcohol metabolism promoters, wakefulness enhancers, and sedatives. For example, modern clinical medicine commonly uses metadoxine to promote ethanol metabolism in the body, combined with naloxone hydrochloride to alleviate the damage of ethanol to the central nervous system; however, it has no clear effect on the gastrointestinal, cardiac, cerebral, and renal damage caused by AAI. Proton pump inhibitors, such as omeprazole, can only reduce the stimulation of the gastrointestinal tract by gastric acid secretion and have many adverse reactions. However, studies have shown that metadoxine has potential teratogenicity and can cause reproductive toxicity; the safety of frequent or long-term use of metadoxine for hangover relief requires further verification. Naloxone has a strong wake-inducing effect and can relieve clinical symptoms in a short time. However, studies show that adverse reactions are mainly cardiovascular (such as arrhythmia and hypertension). Discontinuation of naloxone can cause drowsiness in alcohol-poisoned patients, and some patients may also experience mood abnormalities and blood pressure abnormalities. Omeprazole is primarily metabolized through the liver, thus having a higher potential for liver toxicity. Its side effects also include common gastrointestinal infections, arrhythmias, insomnia, drowsiness, tinnitus, headache, dizziness, mental abnormalities, skin rashes, blood system damage, and damage to the endocrine and reproductive systems. Because factors such as age, gender, liver function, and genetic background can affect alcohol metabolism efficiency, different patients respond significantly to the same treatment regimen. For example, some patients may experience more severe poisoning symptoms due to acetaldehyde dehydrogenase gene defects, making standardized treatment difficult to meet individual needs. Furthermore, even after treatment, many patients still experience residual neurological and digestive discomfort symptoms such as dizziness, headache, nausea, and vomiting. These problems not only affect the quality of the patient's recovery but may also further increase the burden on the liver and delay the overall recovery process. Modern medicine lacks effective means of preventing acute alcohol poisoning.

[0004] In recent years, numerous clinical studies and animal experiments have demonstrated that traditional Chinese medicine (TCM) exhibits unique advantages in the prevention and treatment of acute alcohol poisoning (AAI). For example, CN104147173A discloses a TCM composition for treating acute alcohol poisoning, made from the following raw materials in parts by weight: rhubarb 5-15 parts, kudzu root 10-15 parts, hawthorn 10-15 parts, artemisia capillaris 5-15 parts, bupleurum 3-10 parts, hyacinth bean 10-30 parts, polygala tenuifolia 5-15 parts, and jujube seed 10-15 parts. This composition has the functions of detoxification, liver tonification, and heart and mind calming, and can effectively relieve acute alcohol poisoning and the continued damaging effects of residual alcohol on the body. CN105362384A discloses a TCM composition for relieving hangovers and its application, made from the following raw materials in parts by weight: Japanese raisin tree fruit 3-7 parts, kudzu flower 1-4 parts, magnolia bark flower 0.5-2 parts, and licorice root 1-5 parts. The treatment results were as follows: After taking traditional Chinese medicine, most patients experienced significant improvement in symptoms such as dizziness, nausea, vomiting, thirst, abdominal distension, and restlessness within one hour of drinking, and these symptoms basically disappeared within two hours, with a total effective rate of 100%. Traditional supportive therapies primarily intervene after poisoning occurs, but cannot fundamentally reduce alcohol absorption or accelerate its metabolism, thus failing to effectively prevent and alleviate intoxication.

[0005] Furthermore, on the one hand, there are differences in understanding among those skilled in the art; on the other hand, the applicant studied a large number of documents and patents when making this invention, but due to space limitations, not all details and contents were listed in detail. However, this does not mean that the present invention does not possess the features of these prior art. On the contrary, the present invention already possesses all the features of the prior art, and the applicant reserves the right to add relevant prior art to the background art. Summary of the Invention

[0006] After entering the human body, ethanol is mainly absorbed through the stomach (approximately 20%-30%) and intestines (approximately 70%-80%). After ingestion, ethanol typically enters the bloodstream within 5-10 minutes and reaches peak concentration within 20-60 minutes. Ethanol is distributed throughout all water-containing tissues and fluids in the body, including the brain and alveoli. Approximately 90% of ethanol is metabolized in the liver: first, it is converted to acetaldehyde by alcohol dehydrogenase, and then acetaldehyde is further metabolized to acetic acid by acetaldehyde dehydrogenase. Acetic acid can be completely broken down into carbon dioxide and water in the liver, and can also participate in the body's energy metabolism through other metabolic pathways. The carbon dioxide produced during metabolism is mainly excreted through respiration. In addition, approximately 10% of ethanol is not metabolized and is directly excreted through the kidneys in urine; ethanol and its metabolites may also be excreted through sweat and other routes. Therefore, key measures for preventing and treating acute alcohol poisoning include: protecting the gastrointestinal barrier to slow alcohol absorption; protecting liver function and increasing acetaldehyde dehydrogenase activity, thereby enhancing alcohol metabolism and detoxification; strengthening diuresis to promote alcohol excretion; and minimizing potential adverse reactions from medications. These measures help to effectively reduce the toxic effects of alcohol on the body.

[0007] In view of the shortcomings of the prior art, the first aspect of the present invention provides a composition for preventing and relieving acute drunkenness, the composition being prepared from the following raw materials in parts by weight: 30 parts of kudzu flower, 10 parts of cimicifuga rhizome, 10 parts of creeping sedum, 15 parts of alisma rhizome, 15 parts of atractylodes rhizome, 30 parts of poria cocos, 15 parts of agrimony, 15 parts of galangal, and 30 parts of imperata root.

[0008] According to a preferred embodiment, Atractylodes macrocephala is stir-fried Atractylodes macrocephala and / or processed Atractylodes macrocephala.

[0009] A second aspect of the present invention provides a method for preparing a composition for preventing and alleviating acute intoxication, comprising the following steps:

[0010] Except for Atractylodes macrocephala, the raw medicinal materials in the prescribed amount are soaked in water and then decocted. The decoction is collected and cooled, and then Atractylodes macrocephala enzymatic hydrolysate is added to obtain the composition.

[0011] According to a preferred embodiment, the amount of clean water used is 1.5 times that of the raw medicinal materials.

[0012] According to a preferred embodiment, the soaking time is 2 hours.

[0013] According to a preferred embodiment, the simmering time is 30 minutes.

[0014] According to a preferred embodiment, the medicinal liquid is collected and cooled to 40-50°C before adding Atractylodes macrocephala enzymatic hydrolysate.

[0015] According to a preferred embodiment, the Atractylodes macrocephala enzymatic hydrolysate includes a first Atractylodes macrocephala enzymatic hydrolysate prepared using stir-fried Atractylodes macrocephala and a second Atractylodes macrocephala enzymatic hydrolysate prepared using roasted Atractylodes macrocephala.

[0016] According to a preferred embodiment, the first Atractylodes macrocephala enzymatic hydrolysate is prepared by the following steps:

[0017] After stir-frying Atractylodes macrocephala, it is added to the soaking water and decocted to obtain the first Atractylodes macrocephala liquid.

[0018] Cool the first Atractylodes macrocephala decoction after decoction.

[0019] Enzymatic hydrolysis was carried out by adding cellulase, protease and amylase in a mass ratio of 1.5:1:2;

[0020] The first Atractylodes macrocephala enzymatic hydrolysate was obtained by reflux extraction and purification.

[0021] According to a preferred embodiment, the second Atractylodes macrocephala enzymatic hydrolysate is prepared by the following steps:

[0022] After being honey-fried, Atractylodes macrocephala was added to the soaking water and decocted to obtain the second Atractylodes macrocephala liquid.

[0023] Cool the second Atractylodes macrocephala decoction after decocting;

[0024] Enzymatic hydrolysis was carried out by adding cellulase, protease and amylase in a mass ratio of 1.5:1:2;

[0025] The second Atractylodes macrocephala enzymatic hydrolysate was obtained by reflux extraction and purification.

[0026] According to a preferred embodiment, in the step of preparing the first or second Atractylodes macrocephala extract, the decoction temperature is 70°C.

[0027] According to a preferred embodiment, in the step of preparing the first or second Atractylodes macrocephala extract, the decoction time is 15 minutes.

[0028] According to a preferred embodiment, the first and second Atractylodes macrocephala extracts after decoction are cooled to 40-50°C before enzymatic hydrolysis.

[0029] A third aspect of this invention provides the application of the compositions provided in the first aspect and the methods for preparing the compositions provided in the second aspect of this invention in the preparation of substances that prolong the latency period of intoxication and / or shorten the sobering-up time. Preferably, the substance that prolongs the latency period of intoxication and / or shortens the sobering-up time can be a drug.

[0030] A fourth aspect of the present invention provides the use of the compositions provided in the first aspect of the present invention and the method for preparing the compositions provided in the second aspect of the present invention in the preparation of substances for treating inflammatory diseases. Preferably, the substance for treating inflammatory diseases can be a drug.

[0031] The fifth aspect of the present invention provides the application of the composition provided in the first aspect of the present invention and the method for preparing the composition provided in the second aspect of the present invention in reducing the release level of inflammatory factors.

[0032] According to a preferred embodiment, the inflammatory factors include one or more of interleukin-6, tumor necrosis factor-α, and interleukin-10.

[0033] The sixth aspect of the present invention provides the application of the composition provided in the first aspect of the present invention and the method for preparing the composition provided in the second aspect of the present invention in inhibiting the activation of the transcription activator 3 signaling pathway.

[0034] The seventh aspect of this invention provides the use of the compositions provided in the first aspect of this invention and the method for preparing the compositions provided in the second aspect of this invention in the preparation of a substance for treating liver injury. Preferably, the substance for treating liver injury can be a drug.

[0035] The eighth aspect of the present invention provides the use of the composition provided in the first aspect of the present invention and the method for preparing the composition provided in the second aspect of the present invention in the preparation of a substance for treating gastric mucosal injury. Preferably, the substance for treating gastric mucosal injury can be a drug. Attached Figure Description

[0036] Figure 1 Venn diagram of 19 possible targets of action of the traditional Chinese medicine hangover remedy provided by this invention for treating acute alcohol poisoning;

[0037] Figure 2 The gene ontology analysis results provided by this invention;

[0038] Figure 3 The results of MCODE components extracted via protein interaction networks provided by this invention are shown.

[0039] Figure 4 The enrichment analysis results in DisGeNET provided by this invention;

[0040] Figure 5 The enrichment analysis results in PaGeneBase provided by this invention;

[0041] Figure 6 The enrichment analysis results of TRRUST provided by this invention;

[0042] Figure 7 Statistical results of mouse activity over time provided by this invention;

[0043] Figure 8 The observation results of the behavioral state of mice provided by the present invention;

[0044] Figure 9Statistical results of mouse blood biochemical indicators provided by this invention;

[0045] Figure 10 Statistical results of blood biochemical indicators in mice subjected to acute poisoning experiments for 15 days, as provided by this invention;

[0046] Figure 11 The release levels of IL-6 in the serum of each group of mice provided by this invention;

[0047] Figure 12 The release levels of TNF-α in the serum of each group of mice provided by this invention;

[0048] Figure 13 The release levels of IL-10 in the serum of each group of mice provided by this invention;

[0049] Figure 14 The expression of STAT3 protein in each group of mouse liver tissues provided in this invention was detected by Western blotting.

[0050] Figure 15 The pathological scores of the mouse liver tissues provided by this invention;

[0051] Figure 16 These are pathological tissue sections of mouse liver tissue provided by this invention.

[0052] Figure 17 The pathological scores of gastric mucosal injury in each group of mice provided by this invention;

[0053] Figure 18 Pathological sections of the gastric mucosa of each group of mice provided for this invention. Detailed Implementation

[0054] The present invention will now be described in detail with reference to the accompanying drawings and specific embodiments. It should be understood that these descriptions are exemplary only and are not intended to limit the scope of this disclosure. In the following detailed description, numerous specific details are set forth to provide a thorough understanding of the embodiments of this disclosure for ease of explanation. However, it will be apparent that one or more embodiments may be practiced without these specific details. Furthermore, descriptions of well-known structures and techniques are omitted in the following description to avoid unnecessarily obscuring the concepts of this disclosure.

[0055] All terms used herein (including technical and scientific terms) have the meanings commonly understood by those skilled in the art, unless otherwise defined. It should be noted that the terms used herein are to be interpreted in a manner consistent with the context of this specification, and not in an idealized or overly rigid way.

[0056] In the following embodiments, various processes and methods not described in detail are conventional methods or techniques in the art.

[0057] The pharmacology of this invention is as follows:

[0058] Kudzu flower: This product is sweet, pungent, and cool in nature, and enters the spleen and stomach meridians. It is mainly used to treat thirst and irritability caused by alcohol, headache and dizziness, abdominal distension, vomiting and acid reflux, loss of appetite, hematemesis, and hemorrhage.

[0059] Cimicifuga rhizome: This herb is pungent and slightly sweet in taste, and slightly cold in nature. It enters the lung, spleen, stomach, and large intestine meridians. Cimicifuga rhizome has the effects of releasing exterior pathogens and promoting rash eruption, clearing heat and detoxifying, and raising yang qi. In addition, cimicifuga rhizome and its active ingredients can inhibit the cytokine storm induced by chronic-on-acute liver failure and reduce liver necrosis.

[0060] Sedum sarmentosum: This herb is sweet and bland in taste, cool in nature, and enters the liver, gallbladder, and small intestine meridians. Its main functions are to promote diuresis and relieve jaundice, clear heat and detoxify, and invigorate blood circulation and remove blood stasis.

[0061] Alisma plantago-aquatica: This herb is sweet and bland in taste, cold in nature, and enters the kidney and bladder meridians. It has the functions of promoting diuresis and eliminating dampness, relieving heat, and reducing turbidity and lipids. Its cold nature allows it to clear heat toxins from the body and improve symptoms of febrile diseases, such as thirst, yellow urine, dizziness, vertigo, and weakness in the lower back and knees. It can lower total cholesterol and blood lipids, and has a good therapeutic effect on hyperlipidemia caused by alcohol consumption. Simultaneously, it has an anti-platelet aggregation effect, which helps prevent cardiovascular diseases induced by post-alcohol inflammation. Furthermore, it has certain anti-inflammatory and antibacterial effects, and can assist in the treatment of infections following alcohol-induced damage to the gastrointestinal mucosa.

[0062] Atractylodes macrocephala: This herb is bitter and sweet in taste, warm in nature, and enters the spleen and stomach meridians. Atractylodes macrocephala can invigorate the spleen and replenish qi, enhance spleen and stomach function, and promote digestion; it dries dampness and promotes diuresis, helping to improve damp-heat alcohol toxicity and promote alcohol excretion through urine; it tonifies the middle jiao and replenishes qi, relieving weakness and fatigue after drinking. Atractylodes macrocephala has two processing methods: honey-processed and stir-fried. The similarity is that both retain the basic effects of invigorating the spleen and replenishing qi, drying dampness and promoting diuresis. The difference is:

[0063] Roasted Atractylodes macrocephala: Roasted with honey. Honey is sweet and mild, and has the effects of tonifying the middle energizer, moistening dryness, relieving pain, and detoxifying. After being roasted with honey, Roasted Atractylodes macrocephala has enhanced effects of tonifying the spleen and replenishing qi, and focuses on treating spleen and stomach weakness and insufficient qi, such as fatigue, loss of appetite, abdominal distension, and loose stools. It is more suitable for those with spleen and stomach deficiency and cold.

[0064] Stir-fried Atractylodes macrocephala: After being stir-fried with loess or wheat bran, Atractylodes macrocephala's spleen-strengthening and dampness-drying effects are enhanced. It is often used for spleen deficiency with dampness and water retention, such as edema and phlegm caused by spleen deficiency. Moreover, its warming and drying properties are relatively mild after stir-frying, so it can also be used for those with dampness and a tendency to transform into heat.

[0065] Poria cocos: This herb is sweet, bland, and neutral in nature, and enters the heart, lung, spleen, and kidney meridians. It has the effects of promoting diuresis and eliminating dampness, strengthening the spleen and stomach, and calming the mind and soothing the nerves. Poria cocos polysaccharides have the function of protecting the gastrointestinal mucosa, regulating the spleen and stomach, and relieving vomiting, poor appetite, and indigestion after drinking alcohol; promoting water metabolism and facilitating the excretion of alcohol through urine; it can also relieve restlessness, insomnia, and excessive dreaming, and help improve the excessive inhibition or overactivity of the cerebral cortex after drinking alcohol, thus improving normal sleep quality. In addition, it also has the effects of enhancing immune function and lowering blood sugar.

[0066] Agrimony: This herb is bitter and astringent in taste, neutral in nature, and enters the lung, heart, liver, spleen, and large intestine meridians. Its therapeutic effects mainly include: astringent and hemostatic, anti-diarrheal and antibacterial, tonifying and strengthening, anti-inflammatory and detoxifying, and lowering blood pressure. It can be used for gastrointestinal mucosal bleeding caused by alcohol stimulation, diarrhea and dysentery caused by bacteria, and to relieve symptoms such as weakness after drinking. It also has a vasodilatory effect, which helps relieve symptoms of elevated blood pressure after drinking.

[0067] Galangal: This herb is pungent and hot in nature, and enters the spleen, stomach, and lung meridians. Its main functions are to warm the stomach and stop vomiting, and relieve pain. Galangal can harmonize the stomach and stop vomiting, relieve symptoms such as stomach pain and indigestion caused by alcohol, inhibit ulcer formation, and improve gastrointestinal function.

[0068] Imperata cylindrica root: sweet in taste and cold in nature. It enters the lung, stomach, and bladder meridians, and has the effects of clearing heat and detoxifying, promoting urination and relieving strangury, cooling the blood and stopping bleeding, and reducing internal heat and moistening the lungs. It effectively relieves symptoms such as fever, dry mouth and tongue, and sore throat caused by alcohol-induced damp-heat, and promotes urine production and excretion; it also reduces gastrointestinal mucosal bleeding, inflammation, and pain caused by alcohol. In addition, Imperata cylindrica root also enhances immunity, has antibacterial properties, and lowers blood lipids, blood sugar, and blood pressure.

[0069] Example 1

[0070] This embodiment provides a composition, particularly a composition for preventing and relieving acute intoxication. The composition is prepared from the following raw materials in parts by weight: 30 parts of kudzu flower, 10 parts of cimicifuga rhizome, 10 parts of sedum sarmentosum, 15 parts of alisma rhizome, 15 parts of atractylodes rhizome, 30 parts of poria cocos, 15 parts of agrimony rhizome, 15 parts of galangal root, and 30 parts of imperata rhizome. In this embodiment, parts by weight are expressed in grams. The atractylodes rhizome in this embodiment is either stir-fried or processed; comparative example 5 uses raw atractylodes rhizome.

[0071] This embodiment provides the aforementioned traditional Chinese medicine formula for preventing and alleviating acute alcohol intoxication, in which kudzu flower and cimicifuga are the principal herbs. Kudzu flower relieves alcohol intoxication and invigorates the spleen, effectively treating symptoms such as fever, thirst, headache, dizziness, nausea, acid reflux, and loss of appetite caused by alcohol poisoning. It also has heat-clearing, detoxifying, and diuretic effects, helping to treat symptoms such as diarrhea caused by alcohol-induced intestinal inflammation and promoting the excretion of alcohol through urine. Cimicifuga, in the process of acute alcohol poisoning, can clear heat and detoxify, effectively inhibiting acute liver and gastrointestinal damage caused by alcohol, and has a certain analgesic effect on post-drinking headaches. The combination of kudzu flower and cimicifuga forms a sobering and detoxifying group; one primarily detoxifies alcohol, and the other primarily disperses alcohol toxins, synergistically enhancing the sobering effect and eliminating alcohol toxins from both internal and external sources.

[0072] In this formula, Sedum sarmentosum, Alisma plantago-aquatica, Atractylodes macrocephala, and Poria cocos are all used as assistant herbs. Sedum sarmentosum can alleviate symptoms such as fever, thirst, sore throat, damp-heat in the liver and gallbladder, and jaundice caused by alcohol; it also has a certain relieving effect on headaches and swelling caused by vasodilation. In addition, Sedum sarmentosum also has liver-protecting, transaminase-lowering, pancreatitis-improving, immune-regulating, anti-fatigue, and antioxidant effects. Agrimonia pilosa, Alpinia officinarum, and Imperata cylindrica are all used as adjuvant herbs in this formula. Alisma plantago-aquatica, Poria cocos, and Imperata cylindrica work together to form the spleen-strengthening and dampness-eliminating group, enhancing the effects of clearing heat and dampness, promoting diuresis and detoxification, promoting the excretion of alcohol and its metabolites, and reducing the burden on the liver. Fried / processed Atractylodes macrocephala and Alpinia officinarum work together to form the middle-warming and stomach-harmonizing group, focusing on strengthening the spleen and stomach, warming the middle and dispelling cold, restoring the spleen and stomach's digestive function, resisting the damage of alcohol to the spleen and stomach, relieving gastrointestinal discomfort symptoms after drinking, and balancing cold and heat. The combination of Sedum sarmentosum and Agrimonia pilosa forms a liver-protecting and deficiency-tonifying group. While detoxifying, it also helps to strengthen the body and maintain a good state during the alcohol detoxification process, preventing excessive depletion of vital energy.

[0073] Example 2

[0074] This embodiment provides the analysis process and results of the target points corresponding to the composition provided in Example 1.

[0075] The analysis process in this embodiment is as follows:

[0076] 1. The compounds contained in each herb in the hangover remedy provided in Example 1 were obtained using the TCMSP database. ADME screening was performed on the obtained compounds, where ADME refers to absorption, distribution, metabolism, and excretion. The ADME screening criteria were: oral bioavailability (OB) ≥ 30% and drug-likeness (DL) ≥ 0.18 as key indicators to screen out the effective components of the hangover remedy. Determination of the hangover remedy components and targets: 42 protein targets corresponding to the effective components (compounds) were screened using the STITCH database.

[0077] 2. The correspondence between the components of the hangover remedy provided in Example 1 and their targets was determined. Using the GeneCards database, targets related to alcohol poisoning were collected, merged, and screened. The predicted targets of the hangover remedy components were cross-referenced with relevant targets of acute alcohol poisoning to obtain 19 possible targets for the traditional Chinese medicine hangover remedy in treating acute alcohol poisoning. The results are as follows: Figure 1 As shown. According to this embodiment, when searching the GeneCards database, it is preferable to use "Acute alcoholIntoxication" or "Acute Ethanol Intoxication" as keywords.

[0078] Furthermore, the Metascape and String websites were used to analyze the 19 overlapping target points, and the results are as follows: Figure 2 As shown, Figure A presents the enrichment results for the gene ontology pathway; Figure B presents the enrichment results for the functional pathway. Figures A and B indicate that the 19 targets clustered in the steroid metabolism, organic hydroxy compound metabolism, cholesterol metabolism, and alcohol metabolism pathways, respectively. Figure 2 The results showed that the main mechanisms of action of the hangover remedy provided in Example 1 were the steroid metabolism pathway, which has anti-inflammatory, anti-allergic, protein synthesis-promoting, immunosuppressive, and anti-shock effects; the cholesterol metabolism pathway, which involves stabilizing cell membranes and regulating blood lipids; and the alcohol metabolism pathway, which increases acetaldehyde dehydrogenase.

[0079] Analysis results using the Metascape website, such as Figure 3 As shown, Figure A is the protein interaction network; Figure B is the MCODE component extracted from the protein interaction network. Figure 3 The results showed that the core genes clustered in the steroid pathway and the cholesterol and alcohol metabolism pathways involved in regulating blood lipids.

[0080] In DisGeNET, disease analysis and prediction were performed on 19 targets of hangover remedies for acute alcohol poisoning. The results are as follows: Figure 4 As shown. Figure 4 This indicates that the 19 targets of the hangover remedy provided in Example 1 for treating acute alcohol poisoning are mainly concentrated in the treatment of hyperlipidemia, dementia, hypercholesterolemia, fatty liver, cognitive impairment, cardiovascular and cerebrovascular diseases, etc.

[0081] The results of the target organ analysis in PaGeneBase in this embodiment are as follows: Figure 5 As shown, the results indicate that the liver is the primary target organ for the antidote to acute alcohol poisoning.

[0082] The enrichment analysis results of TRUST are as follows: Figure 6As shown, the transcription factors affected by the hangover remedy provided in Example 1 mainly include SP1, ESR1, RELA (P65), NF-κb1 (P50), and STAT3. Specifically, in the cardiovascular system, ESR1 can lower total cholesterol, increase high-density lipoprotein to regulate dyslipidemia, reduce the formation of oxygen free radicals to combat oxidation, and dilate blood vessels to promote carbon monoxide excretion. On the other hand, ESR1 has a protective effect on the central nervous system: it has a protective effect against various types of nerve damage, including brain injury, oxygen-glucose deprivation, mitochondrial toxins, and glutamate receptor activation, and it regulates synaptic plasticity. In addition, ESR1 has a regulatory effect on pancreatic islet function; for example, it can regulate insulin secretion by pancreatic β cells through direct and indirect effects; ESR1 can also regulate the metabolic processes of bone resorption and bone formation. In a mouse model of Parkinson's disease, inhibiting SP1 expression significantly suppressed the production of inflammatory cytokines, reduced the apoptosis rate, and inhibited the activity of pro-apoptotic regulators (P<0.05); the levels of inflammatory factors TNF-α, IL-1β, and IL-6 were significantly reduced; NF-κB and STAT3 are classic inflammation-related pathways, and inhibiting their activation helps to alleviate widespread inflammatory factor damage and the body's stress response.

[0083] Example 3

[0084] This embodiment provides animal experiments to verify the efficacy of the hangover remedy provided in Example 1.

[0085] The steps for preparing an animal model are as follows:

[0086] Thirty-six male SPF-grade C57 / 6J mice with an average body weight of (23±2) g were selected. After acclimatization, the mice were randomly divided into 6 groups of 5–6 mice each.

[0087] An acute liver injury model was established by administering alcohol via gavage at a dose of 3g / kg twice. The amount of pure alcohol (calculated at 25g / animal) is 25g × 3g / 1000g = 0.075g, which is equivalent to 0.075 / 0.56 / 0.8 = 0.1674ml of 56° Erguotou (g / ml).

[0088] Hangover remedy: For a 60kg adult, drink 150ml at a time, which is 2.5ml / kg. For mice, it is 9 times that of humans, which is 22.5ml / kg. 22.5ml × 25g / 1000g = 0.5625ml. The original formula is 150ml decocted to 50ml. Administer 0.187ml by gavage. After accounting for losses, the gavage dose is 0.2ml each time.

[0089] Grouping is performed as follows:

[0090] A. Acute shock group: 0.2 ml / animal was administered via gavage with normal saline, and 30 min later, 0.34 ml / animal was administered via gavage with 56° Erguotou (0.1674 ml twice).

[0091] B. Acute shock + traditional Chinese medicine group: 0.2 ml / animal was administered of traditional Chinese medicine hangover remedy by gavage, and 30 minutes later, 0.34 ml / animal was administered of 56° Erguotou (0.1674 ml twice).

[0092] C. Acute shock + Metadoxine group: 0.2 ml / animal was administered metadoxine solution by gavage, and 30 min later, 0.34 ml / animal was administered 56° Erguotou liquor by gavage (0.1674 ml twice).

[0093] D. Control group: administered physiological saline twice by gavage.

[0094] E. Acute poisoning group: Traditional Chinese medicine was administered by gavage, 0.2 ml once a day for 15 consecutive days.

[0095] F. Acute poisoning control group: 0.2 ml of normal saline was administered by gavage once a day for 15 consecutive days.

[0096] After treating the mice, this embodiment used the "righting reflex" test to determine the state of intoxication and sobering up, observing the mice's behavior and recording their intoxication and sobering states at different time points (0.5h, 1h, 2h, 3h, 4h, 5h, 6h) to determine the intervention effect of the antidote on intoxicated mice. The recorded intoxication and sobering states of each group were then analyzed... Figure 7 The corresponding relationships shown are converted into scores for analysis. Figure A shows the score relationship between intoxication and sober states; Figure B shows the scores of mice at different time points. Intoxication in mice is defined as the loss of the righting reflex: if a mouse remains in a back-down position for more than 30 seconds, the righting reflex is considered to be lost, indicating intoxication.

[0097] The statistical results of the activity ability and activity status of mice in different treatment groups over time are as follows: Figure 8 As shown. Figure 8To compare the status of mice in the acute shock group, acute shock + traditional Chinese medicine group, acute shock + metadoxine group, and control group, Figure A shows the horizontal axis at different time points and the vertical axis as activity level (i.e., scores corresponding to intoxication and sober states). The activity level of mice in the acute shock group was initially low (score approximately 1), gradually increasing over time, but still significantly lower than the control group. The activity level of mice in the acute shock + traditional Chinese medicine group was initially high (score approximately 2), gradually increasing over time, reaching near normal by 6 hours. Compared to the acute shock + metadoxine group, the acute shock + traditional Chinese medicine group showed higher activity levels and recovered faster over time, indicating that the hangover remedy significantly prolonged the latency period of intoxication and shortened the sobering-up time. Figures B through D show the activity status of mice in the acute shock group, acute shock + traditional Chinese medicine group, acute shock + metadoxine group, and control group at 2 hours, 4 hours, and 6 hours (the control group is not shown).

[0098] After observing the behavioral status of the mice, their blood biochemical indicators were tested. Specifically, after the experiment, blood was collected, serum was separated, and the expression of liver function and blood lipids in the serum was detected. The results are as follows: Figure 9 As shown in the figure, in the acute shock group, alanine aminotransferase (ALT, Figure A) and aspartate aminotransferase (AST, Figure B) were significantly elevated; compared with the acute shock group, ALT and AST were significantly decreased in the acute shock + traditional Chinese medicine group and the acute shock + metadoxine group. Furthermore, the decrease in ALT and AST in the acute shock + traditional Chinese medicine group was greater than that in the acute shock + metadoxine group. In the acute shock group, triglycerides (TG, Figure C) and total cholesterol (TC, Figure D) were elevated; compared with the acute shock group, TG and TC were significantly decreased in the acute shock + traditional Chinese medicine group and the acute shock + metadoxine group.

[0099] This embodiment also included the detection of blood biochemical parameters in mice subjected to acute poisoning experiments after 15 days, and the results are as follows: Figure 10 As shown. Figure 10 The results showed no significant difference in AST (Figure B) between the control group and the acute poisoning group, but the acute poisoning group reduced ALT in mice compared to the control group (p<0.05) (Figure A). There were no significant differences in TG (Figure D) and TC (Figure C) between the control group and the acute poisoning group. Therefore, the traditional Chinese medicine formula for relieving hangovers provided in Example 1 has no significant evidence of toxicity to the body and is a safe hangover remedy.

[0100] In this embodiment, serum samples were also collected from each group of mice. The release levels of the inflammatory factors interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and interleukin-10 (IL-10) in the serum were detected using a Luminex liquid chromatography-mass spectrometry (LC-MS) chip. The results are as follows: Figures 11-13 As shown. Figure 11 The results showed that, compared with the control group, the serum IL-6 release level in the acute shock group was significantly increased. Compared with the acute shock group, the serum IL-6 release level in the acute shock + traditional Chinese medicine group was significantly decreased. Meanwhile, compared with the acute shock + traditional Chinese medicine group, the serum IL-6 release level in the acute shock + metadoxine group was significantly increased. There were statistically significant differences in IL-6 release levels between the acute shock + traditional Chinese medicine group and the acute shock group, as well as between the acute shock + metadoxine group (p<0.05). Figure 12 The results showed that, compared with the control group, the serum TNF-α release level in mice in the acute shock group was significantly increased. Compared with the acute shock group, the serum TNF-α release level in mice in the acute shock + traditional Chinese medicine group was significantly decreased. There was a statistically significant difference in TNF-α release levels between the acute shock + traditional Chinese medicine group and the acute shock group (p<0.05). Figure 13 The results showed that, compared with the control group, the serum IL-10 release level in mice in the acute shock group was significantly increased. Compared with the acute shock group, the serum IL-10 release level in mice in the acute shock + traditional Chinese medicine group was significantly decreased. There was a statistically significant difference in IL-10 release levels between the acute shock + traditional Chinese medicine group and the acute shock group (p<0.05).

[0101] IL-6 and TNF-α are pro-inflammatory factors, while IL-10 is an anti-inflammatory factor. IL-10 secretion is typically driven by NF-κB or STAT3 signaling pathways activated by pro-inflammatory factors (TNF-α, IL-6, etc.). Early anti-inflammatory treatment can rapidly inhibit the release of pro-inflammatory factors, leading to the interruption of IL-10 induction signaling. The function of IL-10 is to suppress excessive inflammation. When anti-inflammatory treatment effectively controls inflammation, the body reduces IL-10 secretion through negative feedback, avoiding excessive immunosuppression. If anti-inflammatory treatment blocks the inflammatory cascade in the early stages, it may directly inhibit subsequent IL-10 expression. Within 24 hours of alcohol-induced acute inflammatory response, IL-10 levels decreased after anti-inflammatory treatment with the hangover remedy provided in Example 1. This decrease in IL-10 levels reflects the effectiveness of the hangover remedy administered via gavage in treating alcohol-induced inflammatory responses. This is indicated by: weakened pro-inflammatory signals leading to reduced IL-10 induction; and reduced IL-10 induction allowing for rapid control of the inflammatory response, eliminating the need for sustained high levels of IL-10 to balance the immune response. Therefore, Figures 11-13 The results showed that the traditional Chinese medicine hangover remedy in Example 1 successfully blocked the positive feedback loop of inflammation.

[0102] STAT3, a key component of the JAK-STAT signaling pathway, plays a crucial role in inflammatory responses. STAT3 participates in the initiation, amplification, and resolution of inflammatory responses by regulating the expression of inflammatory cytokines, cell proliferation, differentiation, and apoptosis. IL-6 is one of the main activators of STAT3. After binding to its receptor, IL-6 phosphorylates STAT3 via JAK kinase, promoting its nuclear translocation and downstream gene transcription. STAT3 activation further upregulates IL-6 expression, forming a positive feedback loop that amplifies the inflammatory response. TNF-α can indirectly activate STAT3 through the NF-κB pathway, enhancing the inflammatory response. STAT3 can also influence the intensity and duration of the inflammatory response by regulating TNF-α expression. IL-10 is an important downstream target gene of STAT3. STAT3 activation promotes IL-10 expression, exerting an anti-inflammatory effect. IL-10, by activating STAT3, inhibits the expression of pro-inflammatory cytokines (such as IL-6 and TNF-α), promoting the resolution of the inflammatory response.

[0103] In this embodiment, liver tissue was collected from each group of mice, proteins were extracted, and the expression of STAT3 protein was detected by Western blotting. Figure 14 As shown. Figure 14 The results showed that the expression level of phosphorylated-STAT3 (p-STAT3) was significantly increased in liver tissue of the acute shock group; compared with the acute shock group, the expression level of phosphorylated-STAT3 (p-STAT3) was significantly decreased in the acute shock + traditional Chinese medicine group and the acute shock + metadoxine group. The STAT3 signaling pathway plays a dual role in the inflammatory response: on the one hand, it amplifies inflammatory signals by promoting the expression of pro-inflammatory factors (IL-6, TNF-α, etc.); on the other hand, it inhibits excessive inflammatory response by upregulating the expression of anti-inflammatory factors (such as IL-10). p-STAT3 maintains the balance of the inflammatory response and prevents excessive inflammatory damage by regulating the expression of pro-inflammatory factors (such as IL-6, TNF-α) and anti-inflammatory factors (such as IL-10). Combined with Luminex inflammatory factor detection (…),… Figures 11-13 The results of Western blot analysis and WB analysis showed that the traditional Chinese medicine formula for relieving hangovers downregulated IL-6 and TNF-α by inhibiting the activation of the STAT3 signaling pathway; the weakening of pro-inflammatory signals led to a reduction in IL-10 induction. Figure 14 The results showed that the inflammatory response caused by acute alcohol injury was effectively controlled in the early stages in both the acute shock + traditional Chinese medicine group and the acute shock + metadoxine group. Targeting the STAT3 signaling pathway may provide a new strategy for the treatment of inflammatory diseases.

[0104] Furthermore, this embodiment also provides observational studies of liver pathological tissues from each group of mice. Specifically, liver tissue samples were taken from each group of mice, fixed with 4% paraformaldehyde, and after good fixation, the samples were trimmed, dehydrated, embedded, sectioned, stained, and mounted in strict accordance with the unit's pathological experimental testing SOP procedures, and finally the qualified samples were examined under a microscope. The slides were viewed under a microscope (upright white light photographic microscope, Nikon (Japan), Eclipse Ci-L), and the tissue sections were observed in detail at different magnifications. The pathological scoring criteria for the slides in this embodiment ([US] Peter. Mann et al. International Standard for Terminology and Diagnostic Criteria of Pathological Changes in Rats and Mice (INHAND) [M]. Translated by Yang Lifeng, Zhou Xiangmei, and Zhao Deming. Beijing: China Agriculture Press, 2019) are as follows: Level 0 is within the normal range (under the study conditions, considering factors such as the age, sex, and strain of the animal, the tissue is considered normal. Changes under other conditions can be considered abnormal); Level 1 is very slight (the changes just exceed the normal range); Level 2 is slight (lesions can be observed, but are not serious); Level 3 is moderate (lesions are obvious and are likely to be more serious); Level 4 is serious (lesions are very serious / lesions have occupied the entire tissue organ). On the other hand, this embodiment assesses pathological tissue based on conditions such as congestion, ecchymosis, hemorrhage, edema, degeneration, necrosis, hyperplasia, fibrosis, organization, granulation tissue, and inflammatory changes. Typical pathological features are marked with arrows of different colors in the photographed slides. The scores and pathological slide results for each group of mice are as follows: Figure 15 and Figure 16 As shown.

[0105] The pathological scoring of mouse liver tissue was performed based on three dimensions: degeneration, necrosis, and inflammatory cell infiltration. Figure 15 The results showed that the acute impact group had the most severe injury, with a mean score of 2.667; the acute impact + metadoxine group had the second most severe injury, with a mean score of 2.0; the acute impact + traditional Chinese medicine group had a mean injury score of 1.200, which was significantly lower than that of the acute impact group (p<0.05). Figure 15 The results showed that the traditional Chinese medicine hangover remedy had a significant protective effect on the liver.

[0106] Representative pathological sections from each group were selected, such as... Figure 16As shown, the scale bar is 100 μm. In the liver tissue of mice in the acute shock + traditional Chinese medicine group, the central vein (black arrow) is visible in the center of the liver lobules, surrounded by hepatocytes (blue arrows) and hepatic sinusoids (yellow arrows) arranged in a roughly radial pattern. The hepatocytes are round and plump; the liver plates are arranged regularly and neatly, and there is no obvious dilation or compression of the hepatic sinusoids; there are no obvious abnormalities in the portal areas between adjacent liver lobules; no obvious inflammatory cell infiltration is observed. In the liver tissue of mice in the acute shock + metadoxine group, numerous hepatocytes with slight vacuolar degeneration (yellow arrows) are visible in the central vein, portal areas, and liver parenchyma, with small, round vacuoles visible in the cytoplasm; many hepatocytes show slight hydropic degeneration, and the cytoplasm is loose and lightly stained; no obvious necrosis or obvious inflammatory cell infiltration is observed. In the liver tissue of mice in the acute shock group, numerous hepatocytes with mild vacuolar degeneration (yellow arrows) were observed around the central vein, portal area, and liver parenchyma. Small, round vacuoles were also visible in the cytoplasm. Occasionally, hepatocyte necrosis (red arrows) and nucleus fragmentation were observed around the blood vessels, and occasional granulocyte infiltration was observed around the cells (blue arrows). Figure 16 The results also show that the traditional Chinese medicine hangover remedy provided in Example 1 has a significant protective effect on the liver.

[0107] In addition, this embodiment also conducted a pathological scoring and observation study of gastric mucosal injury in each group of mice, and the results are as follows: Figure 17 and Figure 18 As shown in the figure. The pathological scores of the gastric tissue of mice in each group were determined based on four dimensions: necrosis, inflammatory cell infiltration, hemorrhage, and gastric gland dilatation. The results are as follows. Figure 17 As shown in the figure. The results indicate that the acute shock + metadoxine group suffered the most severe damage, with a mean score of 3.0; the acute shock group suffered the second most severe damage, with a mean score of 2.0; and the acute shock + traditional Chinese medicine group suffered the least severe damage, with a mean score of 1.200. Therefore, the traditional Chinese medicine hangover remedy provided in Example 1 has a certain protective ability on the gastric mucosa. Representative pathological sections of each group are shown below. Figure 18 As shown.

[0108] Figure 18Pathological sections of gastric mucosa from mice in each group are shown, scale bar = 100 μm. In the acute shock + traditional Chinese medicine group, the gastric mucosa showed minimal epithelial cell necrosis and sloughing (red arrows), with pyknosis, deep staining, and fragmentation of cell nuclei. The lamina propria contained abundant, tightly packed gastric glands, which were tubular glands composed of chief cells and parietal cells. The submucosa was connective tissue. A small amount of lymphocyte infiltration was observed (blue arrows), but no obvious inflammatory cell infiltration was seen. In the acute shock + metadoxine group, the gastric mucosa showed small areas of superficial necrosis (red arrows), loss of epithelial cells and gastric gland structures, slight hemorrhage (yellow arrows), and a small number of erythrocytes with surrounding granulocyte infiltration (blue arrows). The lamina propria contained abundant gastric glands, with slight dilation of the glands (green arrows). The submucosa was connective tissue. In the gastric mucosa tissue of mice in the acute shock group, necrosis and shedding of mucosal epithelial cells were rare in the gastric mucosa layer of the field of view, with condensed, deeply stained, and fragmented cell nuclei; the lamina propria had abundant and tightly arranged gastric glands, which were tubular glands composed of chief cells and parietal cells; the submucosa was connective tissue with a small number of lymphocyte infiltrations (blue arrows); the lamina propria had abundant gastric glands, with slight dilation of gastric glands (green arrows).

[0109] Example 4

[0110] This embodiment provides the preparation method of Atractylodes macrocephala, and process optimization steps such as reflux purification.

[0111] Both stir-fried and roasted Atractylodes macrocephala are Chinese medicinal materials processed from Atractylodes macrocephala using different methods. They differ in their preparation methods and effects, as detailed below:

[0112] (1) Preparation of stir-fried Atractylodes macrocephala

[0113] Select dry, impurity-free, and uniformly sized Atractylodes macrocephala medicinal materials, remove impurities such as fibrous roots, wash and moisten them thoroughly, and then slice them into thin slices with a thickness of 2-4 mm.

[0114] Heat a wok, add an appropriate amount of loess or wheat bran, and heat over medium heat until it smokes. Then add the sliced ​​Atractylodes macrocephala. Stir the slices constantly to ensure they are fully in contact with the loess or wheat bran and heated evenly. The stir-frying time is generally 5-10 minutes, until the surface of the slices turns yellowish-brown, has charred spots, and emits a fragrant aroma. Remove from the wok.

[0115] After stir-frying the Atractylodes macrocephala slices, sift them through a sieve to remove the wheat bran or yellow soil, and let them cool to obtain stir-fried Atractylodes macrocephala.

[0116] (2) Preparation of Atractylodes macrocephala (processed)

[0117] Select high-quality Atractylodes macrocephala, wash and soak it thoroughly, then slice it. Prepare honey; generally, choose high-quality, impurity-free natural honey. If necessary, the honey can be properly refined to make its viscosity and medicinal properties more suitable for the processing requirements.

[0118] Wash and heat a pot, pour in an appropriate amount of refined honey, and heat over low heat. When the honey melts and small, even bubbles appear, add the slices of Atractylodes macrocephala. Gently turn the slices to coat them evenly with honey, and continue heating over low heat. The heating time is usually 10-15 minutes, turning them frequently to prevent them from burning.

[0119] When the Atractylodes macrocephala slices darken in color, become moist and glossy, and are no longer sticky, remove them and air-dry them in a ventilated place or dry them at a low temperature to obtain roasted Atractylodes macrocephala.

[0120] (3) Preparation and purification of the first and second Atractylodes macrocephala enzymatic hydrolysates

[0121] 12g of raw Atractylodes macrocephala was divided into two equal portions and stir-fried or honey-processed separately, using the same preparation methods as (1) and (2). After stir-frying or honey-processing, the portions were added to soaking water and decocted at 70 degrees Celsius for 15 minutes to obtain the first Atractylodes macrocephala liquid and the second Atractylodes macrocephala liquid. The first and second Atractylodes macrocephala liquids were cooled to 40-50℃ respectively, and then cellulase, protease and amylase in a mass ratio of 1.5:1:2 were added for enzymatic hydrolysis. After reflux extraction and purification, the first and second Atractylodes macrocephala enzymatic hydrolysates were obtained.

[0122] Enzymatic hydrolysis of Atractylodes macrocephala using cellulase, protease, and amylase offers numerous benefits, including increasing the extraction rate of active ingredients, enhancing pharmacological activity, improving absorption and utilization, and reducing adverse reactions.

[0123] Example 5

[0124] This embodiment provides a method for preparing the traditional Chinese medicine hangover remedy from Example 1, as follows:

[0125] 30g of kudzu flower, 10g of cimicifuga rhizome, 10g of sedum sarmentosum, 15g of alisma rhizome, 12g of atractylodes macrocephala rhizome, 30g of poria cocos, 15g of agrimony herb, 15g of galangal root, and 30g of imperata root. Except for atractylodes macrocephala rhizome, all other ingredients are soaked separately in 1.5 times their volume of water for 2 hours before being decocted. After decocting for 30 minutes, the liquid is collected, cooled to 40-50℃, and then the first and second atractylodes macrocephala enzymatic hydrolysates are added. This completes the traditional Chinese medicine formula for relieving hangovers.

[0126] Comparative Example 1

[0127] 10g of kudzu flower, 5g of cimicifuga rhizome, 10g of creeping sedum, 15g of alisma rhizome, 12g of atractylodes rhizome, 30g of poria cocos, 15g of agrimony, 15g of galangal, and 30g of imperata root.

[0128] The dosage of the hangover relief and detoxification group was reduced, specifically the amount of kudzu flower and cimicifuga rhizome.

[0129] Comparative Example 2

[0130] 30g of kudzu flower, 10g of cimicifuga rhizome, 10g of sedum sarmentosum, 5g of alisma rhizome, 12g of atractylodes macrocephala rhizome, 5g of poria cocos, 15g of agrimony, 15g of galangal, and 5g of imperata root.

[0131] The dosage of the spleen-strengthening and dampness-eliminating group was reduced, specifically the amount of Alisma plantago-aquatica, Poria cocos, and Imperata cylindrica.

[0132] Comparative Example 3

[0133] 30g of kudzu flower, 10g of cimicifuga rhizome, 10g of sedum sarmentosum, 15g of alisma rhizome, 6g of atractylodes macrocephala rhizome, 30g of poria cocos, 15g of agrimony, 5g of galangal, and 30g of imperata root.

[0134] The comparative proportion reduced the dosage of the warming and stomach-soothing group, that is, reduced the amount of Atractylodes macrocephala and Alpinia galanga.

[0135] Comparative Example 4

[0136] 30g of kudzu flower, 10g of cimicifuga rhizome, 5g of sedum sarmentosum, 15g of alisma rhizome, 12g of atractylodes macrocephala rhizome, 30g of poria cocos, 5g of agrimony, 15g of galangal, and 30g of imperata root.

[0137] The dosage of the liver-protecting and deficiency-tonifying group was reduced, specifically the amount of Sedum sarmentosum and Agrimonia pilosa.

[0138] Comparative Example 5

[0139] 30g of kudzu flower, 10g of cimicifuga rhizome, 10g of sedum sarmentosum, 15g of alisma rhizome, 12g of raw atractylodes macrocephala rhizome, 30g of poria cocos, 15g of agrimony herb, 15g of galangal root, and 30g of imperata root. Soak each ingredient in 1.5 times its volume of water for 2 hours, then decoct for 30 minutes. The resulting liquid is the correct proportion for this hangover remedy.

[0140] Eighteen male drinkers were selected and administered one dose orally before, during, or after drinking. The duration of intoxication and the improvement of TCM symptoms were observed. The specific results are shown in Table 1.

[0141] Table 1

[0142]

[0143] Continued from table:

[0144]

[0145] It should be noted that the specific embodiments described above are exemplary. Those skilled in the art can devise various solutions inspired by the disclosure of this invention, and these solutions all fall within the scope of this invention and its protection. Those skilled in the art should understand that this specification and its accompanying drawings are illustrative and do not constitute a limitation on the claims. The scope of protection of this invention is defined by the claims and their equivalents. This specification contains multiple inventive concepts; phrases such as "preferredly" and "according to a preferred embodiment" indicate that the corresponding paragraph discloses an independent concept. The applicant reserves the right to file divisional applications based on each inventive concept. Throughout the text, the feature introduced by "preferredly" is only an optional mode and should not be construed as mandatory. Therefore, the applicant reserves the right to abandon or delete relevant preferred features at any time.

Claims

1. A composition for preventing and alleviating acute intoxication, characterized in that, The composition is prepared from the following raw materials in parts by weight: 30 parts of kudzu flower, 10 parts of cimicifuga rhizome, 10 parts of sedum sarmentosum, 15 parts of alisma rhizome, 15 parts of atractylodes macrocephala rhizome, 30 parts of poria cocos, 15 parts of agrimony, 15 parts of galangal, and 30 parts of imperata root.

2. The composition according to claim 1, characterized in that, Atractylodes macrocephala is prepared by stir-frying or roasting.

3. The method for preparing the composition for preventing and alleviating acute intoxication according to claim 1, comprising the following steps: Except for Atractylodes macrocephala, the raw medicinal materials in the prescribed amounts are soaked separately in water, then decocted, the decoction is collected and cooled, and then Atractylodes macrocephala enzymatic hydrolysate is added to obtain the composition. Among them, the Atractylodes macrocephala hydrolysate is the first Atractylodes macrocephala hydrolysate prepared by stir-frying Atractylodes macrocephala and the second Atractylodes macrocephala hydrolysate prepared by roasting Atractylodes macrocephala; The first Atractylodes macrocephala enzymatic hydrolysate was prepared by the following steps: Atractylodes macrocephala was stir-fried and then added to soaking water for decoction to obtain the first Atractylodes macrocephala liquid; the first Atractylodes macrocephala liquid was cooled; cellulase, protease and amylase in a mass ratio of 1.5:1:2 were added for enzymatic hydrolysis; reflux extraction and purification were performed to obtain the first Atractylodes macrocephala enzymatic hydrolysate. The second Atractylodes macrocephala enzymatic hydrolysate was prepared by the following steps: Atractylodes macrocephala was honey-processed and then added to soaking water for decoction to obtain the second Atractylodes macrocephala liquid; the decoction was cooled; cellulase, protease and amylase were added in a mass ratio of 1.5:1:2 for enzymatic hydrolysis; reflux extraction and purification were performed to obtain the second Atractylodes macrocephala enzymatic hydrolysate.

4. The use of the composition according to claim 1 or 2, or the method for preparing the composition according to claim 3, in the preparation of a medicament for prolonging the latency period of intoxication and / or shortening the sobering-up time.

5. The application according to claim 4, wherein, The drug prolongs the latency period of intoxication and / or shortens the sobering time by reducing the release level of inflammatory factors.

6. The application according to claim 5, characterized in that, Inflammatory factors include one or more of interleukin-6, tumor necrosis factor-α, and interleukin-10.

7. The application according to claim 4, wherein, The drug prolongs the latency period of intoxication and / or shortens the sobering time by inhibiting the activation of the transcription activator 3 signaling pathway.

8. The use of the composition according to claim 1 or 2, or the method for preparing the composition according to claim 3, in the preparation of a medicament for treating inflammatory diseases caused by acute alcohol injury.

9. The application according to claim 8, wherein, The drug treats inflammatory diseases caused by acute alcohol injury by reducing the release levels of inflammatory factors.

10. The application according to claim 9, characterized in that, Inflammatory factors include one or more of interleukin-6, tumor necrosis factor-α, and interleukin-10.

11. The application according to claim 8, wherein, The composition treats inflammatory diseases caused by acute alcohol injury by inhibiting the activation of the transcription activator 3 signaling pathway.

12. The use of the composition according to claim 1 or 2, or the method for preparing the composition according to claim 3, in the preparation of a medicament for treating acute liver injury caused by alcohol.

13. The use of the composition according to claim 1 or 2, or the method for preparing the composition according to claim 3, in the preparation of a medicament for treating alcohol-induced gastric mucosal damage.

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