Processing method for miniaturization of water lily plants

Abstract

The invention discloses a water lily plant miniaturization treatment method which comprises the following steps: planting water lily fetal seedlings by using a pot sleeving method, recovering the seedlings with clear water for 15-20 days, applying paclobutrazol once every 6-9 days until new root systems grow out and the root systems and soil are staggered, and applying a 0.08-0.15 mL / L paclobutrazol suspending agent with 25% of effective components for three times in total. According to the method, the leaf area and the flower stem of the water lily can be obviously reduced, the flowering number is not influenced, and the plant growth condition is good. The method is easy to operate and convenient to use, can be widely applied to small-sized cultivation of water lily plants, and lays a foundation for production of small-sized water lily potted flowers suitable for family gardening.

Description

Technical Field

[0001] The present invention relates to the field of plant miniaturization, and specifically relates to a method for treating the miniaturization of water lily plants. Background Art

[0002] Water lily is a perennial aquatic herbaceous flower of the genus Nymphaea in the family Nymphaeaceae. It has rich species and high market benefits.

[0003] Nowadays, with the steady progress of people's living standards, there is a greater demand for water lilies, which are ornamental plants. However, in most cases, tropical water lilies are commonly found in the landscaping of ponds, fields, and gardens, and they have a relatively large plant type and need to be planted in large containers, making it difficult to meet the needs of home cultivation and viewing, resulting in a situation where "they can only be viewed from afar, not closely observed". Well-known miniature water lilies such as 'Rwanda' and 'Minuta' have high cultivation costs and low ornamental values. There are also some small water lily varieties, but if not restricted, their leaves will grow outside the flowerpots, and the leaves will wither and die if they do not come into contact with water for a long time, which will in turn affect the growth of the flowers and the entire plant. Therefore, there is an urgent need to find a new method to make the plants of large and medium-sized tropical water lily varieties with high ornamental value smaller.

[0004] Plant growth retardants are a type of plant growth regulators that can dwarf the plants of the treated objects, reduce the leaf area, thicken the leaves, deepen the leaf color, and do not affect the flowering of plants. Common plant growth retardants include paclobutrazol (PP 333 ), chlormequat chloride (CCC), mepiquat chloride (DPC), etc.

[0005] Currently, there is a lack of reports on the method of using plant growth retardants to miniaturize water lilies. An effective treatment method is needed to solve the problems of the relatively large plant type of tropical water lilies and the difficulty of home cultivation. Summary of the Invention

[0006] Aiming at the deficiencies of the prior art, the purpose of the present invention is to provide an effective method for treating the miniaturization of water lily plants, and the present invention solves the problems of the relatively large plant type of existing tropical water lilies and the difficulty of home cultivation.

[0007] To achieve the above purpose, the present invention adopts the following technical solutions:

[0008] A method for treating the miniaturization of water lily plants, comprising the following steps:

[0009] (1) Selection of water lily seedlings and containers: Select pest-free tropical water lily viviparous seedlings for planting. The container is divided into an inner container and an outer container. The inner container is a small container with holes, and the diameter of the inner container is smaller than the diameter of the outer container;

[0010] (2) Hardening off: Plant the viviparous seedlings of water lilies in small containers with holes, lay a layer of non-woven fabric at the bottom, put on an outer container outside, fill the container with water, harden off for 15 - 20 days, and apply slow-release fertilizer.

[0011] (3) Agent selection: Select plant growth retardants.

[0012] (4) Agent application: Pour the plant growth retardant along the periphery of the water lily roots, and stir the water in the container to mix evenly; Agent application time: Add the growth retardant of the same concentration every 6 - 9 days, and apply it 3 times in total.

[0013] (5) Daily maintenance: Scoop up the algae every day and remove the withered and old leaves, and add water to the container to the full regularly.

[0014] Preferably, in step (1), water lilies with viviparous characteristics are required, and select viviparous seedlings without flower buds and with leaf height less than 20 cm and plant them with loam.

[0015] Preferably, the selected container is a small container with a diameter of 12 - 15 cm, which is interplanted in a container with an outer diameter of 30 - 35 cm, and the water level is added to the top of the outer container.

[0016] More preferably, a small container with a diameter of 12 cm can be selected, and then put on a container with an outer diameter of 32 cm and a height of 20 cm.

[0017] When planting in step (2), lay a layer of non-woven fabric in the container with holes, so that it will neither affect soil and water loss during water change nor affect the growth of roots during the growth process.

[0018] Add slow-release fertilizer in the early stage of hardening off. The hardening off time is 15 - 20 days. When new roots grow out and the roots are intertwined with the soil, start to apply the plant growth retardant.

[0019] Preferably, the plant growth retardant in step (3) is paclobutrazol or chlormequat chloride.

[0020] More preferably, the paclobutrazol used is a paclobutrazol suspension with an active ingredient of 25%.

[0021] Preferably, in step (4), add 0.08 - 0.15 ml of paclobutrazol suspension per liter of water. When applying, keep a certain distance from the roots. If it is too close, it is easy to damage the roots. At least keep a distance of 5 cm. After pouring, stir the water in the container evenly.

[0022] Preferably, in step (4), the application time of paclobutrazol is to add the paclobutrazol suspension of the same concentration every 6 days, and apply it three times in total.

[0023] Preferably, during daily maintenance in step (5), dead leaves, rotten leaves and algae in the container should be removed in time, and water should be added to fill the container every 3 days.

[0024] Preferably, the tropical water lily varieties are 'Dober', 'Islamda', 'Black Beauty', 'Small Flower Water Lily', 'Zilag', 'Thai Purple', 'Sugar Rain', 'Blue Bird', 'Lindsay Wood', 'Small Blue Flower', 'Josephine', 'Ruby', 'Paul Orchid', etc.

[0025] A method for treating water lily plants to be miniaturized provided by the present invention has the following beneficial effects compared with the prior art: The present invention can significantly reduce the leaf area and flower stem of water lilies, while not affecting the number of flowers, and the growth condition of the plants is good. The operation of the present invention is simple and convenient to use, and can be widely applied to the cultivation of miniaturized water lily plants, laying a foundation for the production of small-sized water lily potted flowers suitable for home gardening. Description of the Drawings

[0026] Figure 1 Effects of different concentrations of CCC and PP 333 , on the crown width, leaf area, flower branch height, flower branch thickness, flower height, flower diameter, number of flowers, number of leaves, and leaf-flower ratio of tropical water lily 'Dober' under treatments at intervals of 6 days and 9 days.

[0027] Figure 2 Potted plant diagram of 'Dober' under treatment with plant growth retardants.

[0028] Figure 3 Diagram of the maximum length of the whole plant of 'Dober' under treatment with plant growth retardants.

[0029] Figure 4 Effects of different concentrations of CCC and PP 333 , on the crown width, leaf area, flower branch height, flower branch thickness, flower height, flower diameter, number of flowers, number of leaves, and leaf-flower ratio of tropical water lily 'Islamda' under treatments at intervals of 6 days and 9 days.

[0030] Figure 5 Potted plant diagram of 'Islamda' under treatment with plant growth retardants.

[0031] Figure 6 Diagram of the maximum length of the whole plant of 'Islamda' under treatment with plant growth retardants. Detailed Embodiments

[0032] To better illustrate the present invention and facilitate understanding of its technical solutions, the following are typical but non-limiting examples of the present invention. Reagents or equipment not indicating the manufacturer are regarded as conventional products that can be purchased on the market.

[0033] Example 1

[0034] A method for miniaturizing water lily plants is carried out according to the following steps:

[0035] (1) Selection of water lily seedlings and containers: Select viviparous seedlings of tropical water lily 'Dober' without pests and diseases, with consistent growth and no flower buds for planting. Plant 1 seedling per pot. Select a small perforated flower pot with a diameter of 12 cm for the inner layer, and a flower pot with an outer diameter of 32 cm and a height of 20 cm for the outer layer.

[0036] (2) Seedling acclimation: Plant the viviparous seedlings of water lily in a small perforated flower pot with a diameter of 12 cm. Lay a layer of non-woven fabric at the bottom of the pot, and put it in a container with an outer diameter of 32 cm and a height of 20 cm. Fill the container with water and acclimate the seedlings for 15 days. Apply a dozen slow-release fertilizers in the early stage. The slow-release fertilizers are provided by Hainan Lianhua Ecological Culture Co., Ltd. The fertilizer formula: ammonium nitrogen 6.23%, nitrate nitrogen 1.26%, urea 1.95%, other water-soluble nitrogen 0.3%, non-water-soluble nitrogen 2.26%, total nitrogen 12%, available phosphorus 20%, soluble potassium 8%, calcium (Ca) 5%, sulfur (S) 5%, boron (B) 0.07%, copper (Cu) 0.11%, iron (Fe) 0.4%, manganese (Mn) 0.39%, zinc (Zn) 0.22%, chloride ion content not exceeding 2.84%.

[0037] (3) Chemical treatment: Select plant growth retardants chlormequat chloride (CCC, denoted as treatment A, active ingredient 50%. Aqueous solution, Sichuan Runer Technology Co., Ltd.), paclobutrazol (PP 333 , denoted as treatment B, active ingredient 25%. Suspension, Shandong Zouping Pesticide Co., Ltd.). The concentrations of chlormequat chloride CCC are 0.04 ml·L -1 (A1), 0.10 ml·L -1 (A2), 0.16 ml·L -1 (A3), and the concentrations of paclobutrazol PP 333 are 0.04 ml·L -1 (B1), 0.08 ml·L -1 (B2), 0.12 ml·L -1 (B3), and the blank control (ACK) is not treated with chemicals. Both chemicals are treated with two application intervals, 6 days (denoted as 6) and 9 days (denoted as 9) respectively. During the entire test period, a total of three treatments are carried out, and each treatment is repeated with 5 pots.

[0038] (4) Chemical application: Pour the plant growth retardant along the periphery of the water lily roots, and stir the water in the pot to mix evenly.

[0039] (5) Daily maintenance: Scoop up the algae every day and remove the withered and old leaves, and add water regularly until the pot is full.

[0040] (6) Data recording: After processing, the phenotypic indicators were measured at regular intervals for a total of 4 times. The first three measurements were taken at 7-day intervals, and the fourth measurement was taken at a 30-day interval.

[0041] It can be seen from Figure 1 that the plants treated with the plant growth retardant showed obvious changes in the size of the crown diameter compared with the control group. The growth trends of each treatment group gradually slowed down and tended to be stable at different measurement times. On Day 7 after the retardant treatment, PP 333 had a slight advantage over CCC in maintaining the number of flowers and the flower-leaf ratio. On Day 21, the experimental materials absorbed the retardant more fully compared with the previous stage, and the growth gradually slowed down. At the same time, the smallest leaf area L-B 3-6 was reduced by 62.53 cm 2 compared with the control. Among all the indicators, the most significant changes in the flower diameter were observed in L-A 3-6, L-A 3-9, L-B 3-6, and L-B 3-9, which were reduced by 44.43%, 34.44%, 50.94%, and 48.91% compared with the control group (ACK). The highest flower-leaf ratios were found in L-B 2-6, L-B3-6, and L-B1-9. On Day 51, except for ACK without retardant treatment, which continued to show an increasing trend, the growth of each treatment group slowed down and tended to be stable. On Day 51, compared with the control group, the flower branch height, flower branch thickness, flower height, and flower diameter of L-B3-6, which showed the best miniaturization effect among the treatment groups, were reduced by 61.98%, 44.74%, 59.41%, and 63.08%. The highest numbers of flowers and leaves were in the control group (ACK), and there were no differences in the flower-leaf ratios among the groups.

[0042] The measurement results of the maximum length and biomass of the whole plant of 'Duobei' are shown in Table 1. After treatment with the retardant, the maximum length of the whole plant was significantly inhibited. The growth potential of the control group ACK was the strongest, and the inhibitory effect of L-B 3-6 was the strongest, reaching 60.50%. In the weighing of biomass, the fresh and dry weight values of the control group were the highest, and PP 333 fresh and dry < CCC fresh and dry.

[0043] Table 1 Effects of different concentrations of CCC and PP 333 on the maximum length and biomass of the whole plant of tropical water lily 'Duobei' under treatments at 6-day and 9-day intervals

[0044]

[0045] Note: Different lowercase letters only represent significant differences at the 0.05 level (P < 0.05) among different treatments in the same column (the same below).

[0046] The two retardants each have their own advantages in regulating the miniaturization of 'Duobei'. CCC is easy to control, and PP 333The treatment effect is good, and the treatment effect at an interval of 6 days is more significant than that at an interval of 9 days. Phenotypic treatment effect: L-B 3-6 > L-B 3-9 > L-B 2-6 > L-B 2-9 > L-B 1-6 > L-B 1-9 > L-A 3-6 > L-A 3-9 > L-A 2-6 > L-A 2-9 > L-A 1-6 > > L-A1-9 > CK.

[0047] Example 2

[0048] A treatment method for miniaturizing water lily plants is carried out according to the following steps:

[0049] (1) Selection of water lily seedlings and containers: Select viviparous seedlings of tropical water lily 'Islamda' without pests and diseases, with consistent growth and no flower buds for planting. Plant 1 seedling per pot. Select a small flower pot with a diameter of 12 cm and holes inside, and an outer flower pot with an outer diameter of 32 cm and a height of 20 cm.

[0050] (2) Seedling acclimation: Plant the viviparous water lily seedlings in a small flower pot with a diameter of 12 cm and holes. Lay a layer of non-woven fabric at the bottom of the pot, and put it in an outer container with an outer diameter of 32 cm and a height of 20 cm. Fill the container with water and acclimate the seedlings for 15 days. Apply a dozen slow-release fertilizers in the early stage. The slow-release fertilizers are provided by Hainan Lianhua Ecological Culture Co., Ltd. Fertilizer formula: ammonium nitrogen 6.23%, nitrate nitrogen 1.26%, urea 1.95%, other water-soluble nitrogen 0.3%, non-water-soluble nitrogen 2.26%, total nitrogen 12%, available phosphorus 20%, soluble potassium 8%, calcium (Ca) 5%, sulfur (S) 5%, boron (B) 0.07%, copper (Cu) 0.11%, iron (Fe) 0.4%, manganese (Mn) 0.39%, zinc (Zn) 0.22%, chloride ion content not exceeding 2.84%.

[0051] (3) Chemical treatment: Select plant growth retardants chlormequat chloride (CCC, denoted as treatment A, active ingredient 50%. Aqueous solution, provided by Sichuan Runer Technology Co., Ltd.), paclobutrazol (PP 333 , denoted as treatment B, active ingredient 25%. Suspension, provided by Shandong Zouping Pesticide Co., Ltd.). The concentrations of chlormequat chloride CCC are 0.04 ml·L -1 (A1), 0.10 ml·L -1 (A 2), 0.16 ml·L -1 (A 3), and the concentrations of paclobutrazol PP 333 are 0.04 ml·L -1 (B1), 0.08 ml·L -1 (B 2), 0.12 ml·L -1(B 3), the blank control (BCK) was not treated with any agent. Both agents were treated with two application intervals, 6 days (denoted as 6) and 9 days (denoted as 9) respectively. During the entire experimental period, the treatment was carried out three times in total, with 5 pots replicated for each treatment.

[0052] (4) Agent application: Pour the plant growth retardant along the perimeter of the water lily roots and stir the water in the pot to mix evenly. (5) Daily maintenance: Scoop out the algae every day and remove the withered and old leaves, and add water regularly until the pot is full.

[0053] (6) Data recording: Measure the phenotypic indicators at regular intervals after treatment, a total of 4 times. The first three times were at an interval of 7 days, and the fourth time was at an interval of 30 days.

[0054] It can be seen from Figure 4 that the 'Islamda' plants treated with the plant growth retardant showed obvious changes in the size of the crown diameter compared with the control group. The L-A 3-6 and L-B 3-6 of 'Islamda' were reduced by 52.95% and 51.80% respectively compared with the control group. The leaf area on Day 14 increased significantly compared with the previous period, but the growth trend of each treatment group was controlled under the action of the retardant. Among them, the overall growth trend of BCK without retardant treatment was stronger. The treatment effect on the height of the flower branch was the most significant at the same time, and it generally showed a downward trend with the increase of concentration under the treatment of the two retardants. From Day 21 to Day 51, the growth potential and flowering indicators of the treatment group gradually flattened compared with the previous period, and the effect of L-B 3-6 was the greatest.

[0055] The measurement results of the maximum length and biomass of the whole 'Islamda' plant are shown in Table 2. The maximum length of the whole plant was significantly inhibited after treatment with the retardant. It was shown that the growth potential of the control group ACK was the strongest, and the inhibitory effect of L-B 3-6 was the strongest, reaching 72.81%. In the weighing of biomass, the fresh and dry weight values of the control group were the highest, and PP 333 Fresh, dry < CCC fresh, dry.

[0056] Table 2 Effects of different concentrations of CCC and PP 333 , on the maximum length and biomass of the whole plant of tropical water lily 'Islamda' under the treatment with an interval of 6 days and 9 days

[0057]

[0058] The two retardants each have their own advantages in the regulation of the miniaturization of 'Islamda'. CCC is easy to control, and PP 333The treatment effect is good, and the treatment effect at an interval of 6 days is more significant than that at an interval of 9 days. Phenotypic treatment effect: L-B 3-6 > L-B 3-9 > L-B 2-6 > L-B 2-9 > L-B 1-6 > L-B 1-9 > L-A 3-6 > L-A 3-9 > L-A 2-6 > L-A 2-9 > L-A 1-6 >> L-A 1-9 > CK.

[0059] The applicant also tested other tropical water lily varieties such as 'Black Beauty', and all showed the same or similar trends, PP 333 The treatment effect is better, and the treatment effect at an interval of 6 days is more significant than that at an interval of 9 days.

[0060] The protection scope of the present invention is not limited to the above embodiments. Without departing from the spirit and scope of the inventive concept, changes and advantages that can be conceived by those skilled in the art are included in the present invention, and the scope of protection is defined by the appended claims.

Claims

1. A method for miniaturizing water lily plants, characterized in that: The following steps are involved: (1) Selection of water lily seedlings and containers: Select tropical water lily viviparous seedlings that are free of pests and diseases for planting. The containers are divided into inner containers and outer containers. The inner container should be a small container with holes, and the diameter of the inner container should be smaller than that of the outer container. (2) Seedling acclimatization: Plant the water lily viviparous seedlings in a small container with holes, cover the bottom with a layer of non-woven fabric, cover the outside with an outer container, fill the container with water, and let the seedlings grow for 15 to 20 days. Apply slow-release fertilizer in the early stage. (3) Agent selection: Select plant growth retardants; (4) Application of the agent: Pour the plant growth retardant around the water lily root system and stir the water in the container to mix well; Application time: Add the same concentration of paclobutrazol once every 6-9 days, for a total of 3 times; (5) Daily maintenance: Collect algae and remove old leaves every day, and add water regularly to fill the container.

2. The method for miniaturizing water lily plants according to claim 1, characterized in that: In the step (1), viviparous seedlings without flower buds and with leaf height less than 20 cm are selected and planted in loam.

3. The method for miniaturizing water lily plants according to claim 1, characterized in that: The inner container in step (1) is a small container with a hole and a diameter of 12-15 cm, and the outer container has an outer diameter of 30-35 cm.

4. The method for miniaturizing water lily plants according to claim 1, characterized in that: The plant growth retardant in step (3) is paclobutrazol or chlormequat chloride.

5. The method for miniaturizing water lily plants according to claim 1, characterized in that: The plant growth retardant in step (3) is paclobutrazol, and the paclobutrazol suspension containing 25% of the active ingredient is used.

6. The method for miniaturizing water lily plants according to claim 1, characterized in that: In the step (4), 0.08-0.15 ml of paclobutrazol suspension is added per liter of water.

7. The method for miniaturizing water lily plants according to claim 1, characterized in that: In the step (4), paclobutrazol is applied once every 6 days.

8. The method for miniaturizing water lily plants according to claim 1, characterized in that: During routine maintenance in step (6), dead leaves, rotten leaves and algae in the container should be removed promptly, and water should be added every 3 days until the container is full.

9. The method for miniaturizing water lily plants according to claim 1, characterized in that: The tropical water lily variety is selected from any one of 'Dobe', 'Islamida', 'Black Beauty', 'Small Flower Water Lily', 'Qilage', 'Thai Purple', 'Sugar Rain', 'Blue Bird', 'Lindsay Wood', 'Little Blue Flower', 'Josephine', 'Ruby', and 'Paul Lan'.

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