Preparation method of acarus killing plant combination liquid

Abstract

The invention discloses a preparation method of a mite-killing plant combined liquid. The method comprises the following steps: crushing fingered citron, lotus leaves, folium artemisiae argyi, thyme, liquorice, cortex moutan and lavender; the crushed raw materials are mixed according to a certain proportion and then extracted, and then the acarus killing plant combination liquid with the concentration of 5-20% is prepared according to the proportion. The acarus killing plant combination liquid prepared by the scheme has a good acarus killing effect, the acarus killing death rate of each embodiment at different time points basically presents a rising trend along with the increase of the concentration of the combination liquid, the acarus killing rate reaches 90% or above within 72 hours, and the acarus killing plant combination liquid reaches the standard of commercially available products. Compared with single fingered citron, lotus leaf, folium artemisiae argyi, thyme, liquorice, cortex moutan and lavender extracting solutions, the combined solution in the scheme has a remarkable mite killing effect, and data show that all the raw materials have a synergistic effect. Meanwhile, the acarus killing plant combination liquid prepared by the scheme is high in use safety and simple in preparation process, and has wide market prospects and important practical significance.

Description

Technical Field

[0001] The present invention relates to the field of acaricidal liquids, and more specifically, to a preparation method of a plant combination acaricidal liquid. Background Art

[0002] Mites are common microorganisms that widely exist in household environments such as beds, sofas, carpets, etc., as well as on the surface of human skin. The breeding of mites can cause many health problems, such as skin allergies, itching, swelling, and respiratory diseases such as asthma, seriously affecting people's quality of life.

[0003] At present, there are various types of acaricidal products on the market, mainly including chemically synthesized acaricides and some physical acaricidal methods. Although chemically synthesized acaricides have relatively significant acaricidal effects, they often have certain toxicity and are prone to pose potential threats to human health, especially for sensitive populations such as the elderly, children, and pregnant women. Moreover, some chemically synthesized acaricides may cause environmental pollution and damage the ecological balance. Physical acaricidal methods such as high-temperature exposure and ultraviolet irradiation are relatively safe, but they are inconvenient to operate and the acaricidal effects are not thorough and lasting enough. Therefore, developing a natural, safe, efficient, and environmentally friendly plant combination acaricidal liquid has broad market prospects and important practical significance. Summary of the Invention

[0004] The purpose of the present invention is to provide a preparation method of a plant combination acaricidal liquid to solve the technical problems existing in the above background art.

[0005] The technical solution of the present invention provides a preparation method of a plant combination acaricidal liquid, including the following steps:

[0006] S1: Put the dried bergamot, lotus leaf, mugwort leaf, thyme, licorice root, moutan bark, and lavender into a traditional Chinese medicine grinder respectively, and grind them into powders with a particle size of 100 - 120 mesh.

[0007] S2: Mix the powders of each raw material in a certain proportion; fully mix the organic solvent and the mixed raw material powders, and use the organic solvent to soak and extract them in a closed container.

[0008] S3: After the soaking extraction is completed, connect the closed container to a heating reflux device, slowly raise the temperature to the state where the solvent is slightly boiling, control the temperature between 60 - 80°C, and keep refluxing and extracting for 2 - 4 hours.

[0009] S4: After the heating reflux extraction is completed, quickly filter the extract through double-layer gauze to remove larger particles of residues, such as plant fibers and unbroken cell tissues. Then, the initially filtered extract is further filtered through a vacuum filtration device using a microporous membrane with a pore size of 0.22 - 0.45 μm to obtain a plant combination extract.

[0010] S5: Prepare the above plant combined extract into a mite - removing plant combined liquid with a concentration of 5 - 20% according to a ratio.

[0011] In a preferred embodiment, each raw material in S1 is dried to more than 95%, and the rotation speed of the pulverizer is 2000 - 3000 r / min.

[0012] In a preferred embodiment, the organic solvent in S2 is a mixture of ethanol and glycerol. The volume fraction of ethanol is 50 - 60%, the volume fraction of glycerol is 20 - 30%, and glycerol accounts for 5 - 10% of the total volume of the solution.

[0013] In a preferred embodiment, the weight of the organic solvent in S2 is 8 - 12 times the weight of the raw material powder.

[0014] In a preferred embodiment, the extraction temperature in S2 is 25 - 35 °C, the soaking time is 24 - 48 h, and stirring is carried out every 4 - 6 h.

[0015] In a preferred embodiment, the raw material powders are mixed according to the following weight ratio: bergamot: lotus leaf: mugwort leaf: thyme: licorice: moutan bark: lavender = (1 - 3):(1 - 3):(1 - 3):(1 - 3):(1 - 2):(1 - 2):(1 - 3).

[0016] In a preferred embodiment, the raw material powders are mixed according to the following weight ratio: bergamot: lotus leaf: mugwort leaf: thyme: licorice: moutan bark: lavender = 1:1:1:1:1:1:1.

[0017] The beneficial effects of the technical solution of the present invention are as follows:

[0018] The mite - removing plant combined liquid prepared by this solution has a good mite - removing effect. As the concentration of the combined liquid increases, the mite mortality rate at different time points in each example basically shows an upward trend, and the mite - removing rate reaches more than 90% after 72 hours, meeting the standards of commercially available products. Compared with the single extracts of bergamot, lotus leaf, mugwort leaf, thyme, licorice, moutan bark, and lavender, the combined liquid of this solution has a significant mite - removing effect, and it can be seen from the data that there is a synergistic effect among the raw materials. At the same time, the mite - removing plant combined liquid prepared by this solution has high use safety and a simple preparation process, and has broad market prospects and important practical significance. Detailed implementation manners

[0019] The present invention will be further described in detail below. The embodiments of the present invention are given for the convenience of illustration and description, and are not exhaustive or limit the present invention to the disclosed form. Many modifications and variations are obvious to those of ordinary skill in the art. The embodiments are selected and described to better illustrate the principles and practical applications of the present invention, and enable those of ordinary skill in the art to understand the present invention and thus design various embodiments with various modifications suitable for specific purposes.

[0020] The technical solution of the present invention provides a preparation method of a mite-removing plant combination liquid, including the following steps:

[0021] S1: Put the dried bergamot, lotus leaf, mugwort leaf, thyme, licorice root, cortex moutan, and lavender into a traditional Chinese medicine grinder respectively, and grind them into powders with a particle size of 100-120 meshes; each raw material in S1 is dried to more than 95%, and the rotation speed of the grinder is 2000-3000 r / min.

[0022] S2: Mix the powders of each raw material in a certain proportion; fully mix the organic solvent and the mixed raw material powders, and use the organic solvent to soak and extract in a closed container; the organic solvent is a mixture of ethanol and glycerol, the volume fraction of ethanol is 50-60%, the volume fraction of glycerol is 20-30%, and glycerol accounts for 5-10% of the total volume of the solution. In S2, the weight of the organic solvent is 8-12 times the weight of the raw material powders. The extraction temperature in S2 is 25-35°C, the soaking time is 24-48 h, and stirring is carried out every 4-6 h to promote the full contact between the solvent and the raw materials and improve the extraction efficiency.

[0023] S3: After the soaking extraction is completed, connect the closed container to a heating reflux device, slowly raise the temperature to the state where the solvent is slightly boiling, control the temperature between 60-80°C, and maintain reflux extraction for 2-4 h; during the reflux process, the solvent continuously evaporates and condenses in the condenser and then refluxes into the extraction container to form a cycle, so that the active ingredients in the plant cells can be more fully dissolved in the solvent. At the same time, in order to prevent the occurrence of bumping phenomena, an appropriate amount of zeolite or magnetic stirrer can be added to the container, and the stirring device is turned on to make the extraction process more stable and uniform.

[0024] S4: After the heating reflux extraction is completed, quickly filter the extraction liquid through double-layer gauze to remove larger particles of residues, such as plant fibers and unbroken cell tissues. The extraction liquid after preliminary filtration is then finely filtered through a pressure filtration device using a microporous membrane with a pore size of 0.22-0.45 μm to obtain a plant extraction liquid.

[0025] S5: Configure the above-mentioned plant combination extraction liquid into a mite-removing plant combination liquid with a concentration of 5-20%.

[0026] In S2, the raw material powders are mixed according to the following weight ratios: bergamot: lotus leaf: mugwort leaf: thyme: liquorice: moutan bark: lavender = (1-3):(1-3):(1-3):(1-3):(1-2):(1-2):(1-3).

[0027] Example 1

[0028] The technical solution of the present invention provides a method for preparing a mite-removing plant combination liquid, comprising the following steps:

[0029] S1: The dried bergamot, lotus leaf, mugwort leaf, thyme, liquorice, moutan bark, and lavender are respectively put into a traditional Chinese medicine grinder and ground into powders with a particle size of 100-120 meshes;

[0030] S2: The powders of the raw materials are mixed in a certain ratio; the organic solvent and the mixed raw material powders are fully mixed, and the mixture is soaked and extracted with the organic solvent in a closed container;

[0031] S3: After the soaking extraction is completed, the closed container is connected to a heating reflux device, and the temperature is slowly raised to the state of slight boiling of the solvent, the temperature is controlled between 60-80 °C, and the reflux extraction is maintained for 2-4 h;

[0032] S4: After the heating reflux extraction is completed, the extract is preliminarily filtered through double-layer gauze while it is hot to remove larger particles of residues, such as plant fibers and unbroken cell tissues. The preliminarily filtered extract is then finely filtered through a pressure-reducing suction filtration device using a microporous membrane with a pore size of 0.22-0.45 μm to obtain a plant extract.

[0033] S5: The above-mentioned plant combination extract is respectively formulated into mite-removing plant combination liquids with concentrations of 5%, 10%, and 20%.

[0034] In S1, each raw material is dried to more than 95%, and the rotation speed of the grinder is 2500 r / min.

[0035] In S2, the organic solvent is a mixture of ethanol and glycerol. The volume fraction of ethanol is 55%, the volume fraction of glycerol is 25%, and glycerol accounts for 5% of the total volume of the solution. The weight of the organic solvent in S2 is 10 times the weight of the raw material powders. The extraction temperature in S2 is 30 °C, the soaking time is 36 h, and stirring is carried out once every 5 h.

[0036] The raw material powders are mixed according to the following weight ratios: bergamot: lotus leaf: mugwort leaf: thyme: liquorice: moutan bark: lavender = 1:1:1:1:1:1:1.

[0037] Example 2;

[0038] The difference from Example 1 is that the ratio of fingered citron: lotus leaf: mugwort leaf: thyme: liquorice root: moutan bark: lavender is 1:3:2:2:1:1:2, and the mite-removing plant combined solution with concentrations of 5%, 10% and 20% is respectively prepared.

[0039] Example 3;

[0040] The difference from Example 1 is that the ratio of fingered citron: lotus leaf: mugwort leaf: thyme: liquorice root: moutan bark: lavender is 2:2:3:1:2:1:1, and the mite-removing plant combined solution with concentrations of 5%, 10% and 20% is respectively prepared.

[0041] Example 4;

[0042] The difference from Example 1 is that the ratio of fingered citron: lotus leaf: mugwort leaf: thyme: liquorice root: moutan bark: lavender is 3:1:1:3:1:2:2, and the mite-removing plant combined solution with concentrations of 5%, 10% and 20% is respectively prepared.

[0043] Example 5;

[0044] The difference from Example 1 is that the ratio of fingered citron: lotus leaf: mugwort leaf: thyme: liquorice root: moutan bark: lavender is 1:2:3:2:2:1:3, and the mite-removing plant combined solution with concentrations of 5%, 10% and 20% is respectively prepared.

[0045] Comparative Example 1

[0046] Use a single fingered citron extract as the mite-removing plant combined solution.

[0047] Comparative Example 2

[0048] Use a single lotus leaf extract as the mite-removing plant combined solution.

[0049] Comparative Example 3

[0050] Use a single mugwort leaf extract as the mite-removing plant combined solution.

[0051] Comparative Example 4

[0052] Use a single thyme extract as the mite-removing plant combined solution.

[0053] Comparative Example 5

[0054] Use a single liquorice root extract as the mite-removing plant combined solution.

[0055] Comparative Example 6

[0056] Use a single moutan bark extract as the mite-removing plant combined solution.

[0057] Comparative Example 7

[0058] Use a single lavender extract as the mite-removing plant combination liquid.

[0059] Commercially available products

[0060] Use purchased commercially available products as the mite-removing plant combination liquid.

[0061] Mite-removing experiment

[0062] Conduct a mite-removing experiment on the mite-removing plant combination liquids of Examples 1-5 and Comparative Examples 1-8.

[0063] (I) Mite culture and preparation

[0064] Dermatophagoides farinae culture: In a specific incubator, maintain the temperature at 26°C and the relative humidity at 75%. Use fully dried human dander as the food source to culture a large number of Dermatophagoides farinae. During the culture process, regularly check the growth status of the mites to ensure that their quantity is sufficient and their activity is stable.

[0065] Mite collection and counting: Use a soft brush to gently collect the active mites from the incubator and place them in a sterile petri dish. Under the microscope, use an accurate counting method to determine that the number of mites in each petri dish is approximately 1000. Subsequently, place the petri dish containing the mites in a constant temperature and humidity incubator (temperature 26°C, relative humidity 75%) for 30 minutes to allow the mites to adapt to the environment.

[0066] Mite inoculation: Take out 6 cotton cloth pieces with a size of 6 cm × 6 cm. Under the microscope, evenly inoculate the mites onto each cloth piece, with 500 mites inoculated on each piece. After inoculation, place the cloth pieces in new petri dishes for standby.

[0067] All the above test samples have passed strict skin irritation tests and subsequent experiments are carried out after passing.

[0068] The mite-removing grade is judged according to the following criteria: the standard of NY / T 1151.2-2006 "Indoor efficacy test and evaluation of pesticides for hygienic use - Part 2: Acaricides and acarid repellents". Acaricide A level (acaricidal rate = 100%), acaricide B level (acaricidal rate ≥ 95%), acarid repellent A level (acarid repellent rate ≥ 95%), acarid repellent B level (acarid repellent rate ≥ 90%).

[0069] (II) Treatment with the combination liquid

[0070] Use a sprayer to evenly spray the solutions of different groups on the corresponding mite-inoculated cloth pieces. The spraying amount for each cloth piece is strictly controlled at 2 mL to ensure that the surface of the cloth piece is completely wet but there is no obvious liquid accumulation. After spraying, place the cloth pieces in a fume hood to dry naturally for 10 minutes.

[0071] (IV) Mite observation and counting

[0072] Incubator placement: Place the treated cloth pieces in a constant temperature and humidity incubator (temperature 26°C, relative humidity 65%).

[0073] Regular observation: At 24 hours, 48 hours, and 72 hours after treatment, take out the cloth pieces from the incubator, gently transfer the mites on the cloth pieces to a glass slide with forceps, add a drop of physiological saline, and cover with a cover slip.

[0074] Microscope counting: Observe and count the number of surviving mites under the microscope. The magnification of the microscope can be continuously adjusted between 100 - 1000 times to clearly observe the morphology and activity status of the mites. Randomly select 5 fields of view for each cloth piece to count, and take the average value as the number of mites on that cloth piece. Then calculate the mite mortality rate according to the formula: Mite mortality rate = (Number of mites before treatment - Number of mites after treatment) / Number of mites before treatment × 100%.

[0075] Criterion for judging mite survival: Mites are judged to be alive if their limbs can move normally, their body structure is intact, and they have an obvious reaction to slight touch (such as gently touching with a fine needle); conversely, mites with stiff, curled, or broken limbs, shriveled bodies, and no reaction to touch are judged to be dead.

[0076] The mite mortality rates of each group at 24 hours, 48 hours, and 72 hours are shown in Table 1 below:

[0077] Table 1

[0078]

[0079]

[0080] As can be seen from Table 1 above, the mite - removing plant combination liquid prepared by this scheme has a good mite - removing effect. As the concentration of the combination liquid increases, the mite mortality rates of each example at different time points basically show an upward trend. The mite - removing rate at 72 hours reaches more than 90%, meeting the standards of commercially available products. The mite mortality rate of Example 2 is generally high at each concentration and time point. Especially at higher concentrations (10 and 20), the mite mortality rate can reach 100% at 24 hours. At all time points (24, 48, 72 hours) and concentration combinations, the mite mortality rate is very high, fully meeting the A - level standard for acaricides (mite - removing rate = 100%), indicating that the combination liquid formula of this experimental group has strong acaricidal ability.

[0081] Compared with single extracts of bergamot, lotus leaf, mugwort leaf, thyme, licorice, moutan bark, and lavender, the combination liquid of this scheme has a significant mite - removing effect, and it can be seen from the data that there is a synergistic effect among the raw materials. At the same time, the mite - removing plant combination liquid prepared by this scheme has high safety in use and a simple preparation process, with broad market prospects and important practical significance.

[0082] Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all embodiments. All other embodiments obtained by those of ordinary skill in the art and related fields based on the embodiments of the present invention without creative efforts shall fall within the scope of protection of the present invention. Structures, devices, and operation methods not specifically described and explained in the present invention shall be implemented by conventional means in the art unless otherwise specified and limited.

Claims

1. A method for preparing a mite-killing plant combination liquid, characterized in that: The steps include: S1: Put the dried bergamot, lotus leaf, wormwood leaf, thyme, licorice, moutan bark and lavender into a traditional Chinese medicine grinder respectively, and grind them into powder with a particle size of 100-120 mesh; S2: mixing the powders of the raw materials in a certain proportion; fully mixing the organic solvent and the mixed raw material powders, and soaking and extracting them in a closed container with the organic solvent; S3: After the soaking extraction is completed, the sealed container is connected to a heating reflux device, and the temperature is slowly raised to a slightly boiling state of the solvent, and the temperature is controlled between 60-80°C, and the reflux extraction is maintained for 2-4 hours; S4: After the heating reflux extraction is completed, the extract is preliminarily filtered through a double-layer gauze while hot to remove larger particles of residue, such as plant fibers and unbroken cell tissues. The extract after the preliminary filtration is then finely filtered through a vacuum filtration device using a 0.22-0.45 μm microporous filter membrane to obtain a plant combination extract; S5: The above plant combination extract is prepared into a mite-killing plant combination liquid with a concentration of 5-20% according to a certain ratio.

2. The method for preparing a mite-killing plant combination liquid according to claim 1, characterized in that: The raw materials in S1 are dried to more than 95%, and the rotation speed of the pulverizer is 2000-3000r / min.

3. The method for preparing a mite-killing plant combination liquid according to claim 1, characterized in that: The organic solvent in S2 is a mixture of ethanol and glycerol, the volume fraction of ethanol is 50-60%, the volume fraction of glycerol is 20-30%, and the glycerol accounts for 5-10% of the total volume of the solution.

4. The method for preparing a mite-killing plant combination liquid according to claim 1, characterized in that: The weight of the organic solvent in S2 is 8-12 times the weight of the raw material powder.

5. The method for preparing a mite-killing plant combination liquid according to claim 1, characterized in that: The extraction temperature in S2 is 25-35°C, the soaking time is 24-48h, and stirring is performed every 4-6h.

6. The method for preparing a mite-killing plant combination liquid according to claim 1, characterized in that: The raw material powders are mixed according to the following weight ratio: bergamot: lotus leaf: mugwort leaf: thyme: licorice: moutan bark: lavender = (1-3): (1-3): (1-3): (1-3): (1-2): (1-2): (1-3).

7. The method for preparing a mite-killing plant combination liquid according to claim 6, characterized in that: Mix the raw material powders according to the following weight ratio: bergamot: lotus leaf: mugwort leaf: thyme: licorice: Moutan bark: lavender = 1:1:1:1:1:1:1:1.

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