Layered monitoring experimental device for simulating influence of antibiotic input on underground water denitrification and microbial sampling method thereof

Abstract

The invention provides a stratified monitoring experimental device for simulating the influence of antibiotic input on underground water denitrification and a microbial sampling method thereof, and belongs to the technical field of underground water pollution control experiments. The layered monitoring experimental device for simulating the influence of antibiotic input on underground water denitrification mainly comprises underground water simulation experimental columns, a water supply system, a simulation underground water sampling system, a microorganism sampling system and a supporting platform, and the underground water simulation experimental columns are all arranged on the supporting platform; the underground water simulation experiment column is connected with a water supply bottle through a silica gel hose and a peristaltic pump; the simulated underground water sampling system comprises a plurality of simulated underground water sampling ports and pore water samplers; the microorganism sampling system comprises a plurality of microorganism sampling ports, a porous support and a sampling swab. The device can simulate the influence of antibiotic input on the denitrification process of underground water, and can monitor water quality and microorganisms in a layered manner. The experimental device is simple in structure, small in occupied area and suitable for being popularized in laboratories.

Description

Technical Field

[0001] The present invention relates to the technical field of groundwater pollution control experiments, and particularly relates to a layered monitoring experimental device for simulating the influence of antibiotic input on groundwater denitrification and a method for sampling microorganisms thereof. Background Art

[0002] In recent years, some studies have shown that there are various pollutants in the groundwater environment. Due to the excessive use of chemical fertilizers and the discharge of industrial wastewater, etc., serious nitrate pollution has occurred in groundwater. In addition to nitrates, antibiotics are typical organic pollutants in groundwater. Since sewage treatment plants cannot completely remove them, antibiotics will enter groundwater through migration pathways such as percolation and leaching.

[0003] Generally speaking, the denitrification process in groundwater is realized by the corresponding microorganisms driving the nitrate degradation process under anaerobic or facultative anaerobic conditions, and the antibacterial and bacteriostatic properties of antibiotics will affect the microorganisms in groundwater. Therefore, correctly understanding the influence of antibiotics on the denitrification process in the shallow aquifer under different input modes and clarifying the influence mechanism of antibiotics on denitrification have important significance and practical value for antibiotic emission management and ecological environment protection.

[0004] At present, there are few studies on column experiments of the groundwater denitrification process, and it is difficult to extract microorganisms in simulated groundwater layer by layer for microscopic analysis. Therefore, it is necessary to provide a layered monitoring experimental device for simulating the influence of antibiotic input on groundwater denitrification and a method for sampling microorganisms thereof to solve the above problems. Summary of the Invention

[0005] The object of the present invention is to overcome the above problems and develop a layered monitoring experimental device for simulating the influence of antibiotic input on groundwater denitrification. This device can simulate the migration and transformation of pollutants such as antibiotics in the groundwater dynamic process and their influence behavior on microbial denitrification. Generally speaking, the results obtained by only setting one experimental column are not accurate enough. This device sets four groundwater simulation columns as experimental groups, which can be used as experimental groups under different conditions and parallel experimental control groups, making the experimental results more accurate.

[0006] To achieve the above experimental objectives, a layered monitoring experimental device for simulating the impact of antibiotic input on groundwater denitrification according to the present invention has the following main technical features: a groundwater simulation experimental column, a water supply system, a simulated groundwater sampling system, a microorganism sampling system, and a support platform. The four groundwater simulation experimental columns are all placed on the support platform. The groundwater simulation experimental column is connected to a water supply water bottle through a silica gel hose and a peristaltic pump, and continuous water inflow is achieved by replacing the water supply water bottle. There are four vertically distributed sampling ports with different intervals on the side of the groundwater simulation experimental column. One of them is a simulated groundwater sampling port, and the other three are microorganism sampling ports. The water supply system includes the water inlets of the groundwater simulation experimental columns, silica gel hoses, water supply water bottles, and peristaltic pumps. Each experimental column is equipped with its own silica gel hose and water supply water bottle, and water is introduced through the same peristaltic pump to ensure the same water inflow rate. The simulated groundwater sampling system includes multiple simulated groundwater sampling ports and pore water samplers. The pore water samplers are horizontally inserted into the simulated groundwater sampling ports. The microorganism sampling system includes multiple microorganism sampling ports, a porous support, and sampling swabs. When the medium in the experimental column is filled to the height of each microorganism sampling port, the porous support is placed, and multiple sampling swabs are placed in the hollow position of the porous support.

[0007] Preferably, the upper and lower parts of the groundwater simulation column are both designed with openings. Nylon nets or gauzes can be placed at the water inlet and outlet to prevent the medium from blocking the water inlet and outlet. The top water outlets of the four experimental columns are respectively connected to a water outlet water bottle through silica gel hoses to collect the effluent of the experimental columns. The water outlet water bottle can be equipped with an aluminum foil sampling bag to collect gas samples. The bottom water inlet is connected to the water supply system.

[0008] Preferably, the groundwater simulation columns are arranged side by side on the support platform. There are four vertically arranged sampling ports in the four directions of the front, back, left, and right on the side wall of each experimental column. The distance from the center of the sampling port to the bottom of the experimental column gradually increases from bottom to top. One of them is a simulated groundwater sampling port, which is designed with an opening; the other three are microorganism sampling ports. Each microorganism sampling port is equipped with a threaded cap, and there is a cylinder in the center of the threaded cap, which can just block the vertical sampling port space.

[0009] Preferably, the water supply system includes the water inlets of the groundwater simulation experimental columns, silica gel hoses, water supply water bottles, and peristaltic pumps. Each experimental column is equipped with its own silica gel hose and water supply water bottle, and water is introduced through the same peristaltic pump to ensure the same water inflow rate. The water supply water bottle can be equipped with an air bag to balance the air pressure in the bottle.

[0010] Preferably, the simulated groundwater sampling system includes several simulated groundwater sampling ports and pore water samplers. The pore water samplers are vertically inserted and placed in the simulated groundwater sampling ports, and the simulated groundwater sampling ports can be sealed and wrapped with a sealing film.

[0011] Preferably, the microbial sampling system includes several microbial sampling ports, a porous support, and sampling swabs. The porous support consists of two circular plates with an outer diameter slightly smaller than the inner diameter of the groundwater simulation experimental column, with a thickness of 3 mm. The circular plates are evenly distributed with small holes with a diameter of 2 mm. The two circular plates are supported by four small cylinders to form the porous support. When the medium in the experimental column is filled to the height of each microbial sampling port, the porous support is placed. The vertical columnar microbial sampling ports correspond one by one to the hollow positions of the two circular plates of the porous support. A nylon mesh or gauze is placed on the upper part of the porous support to prevent the medium from sinking due to gravity. Multiple sampling swabs are placed in the hollow position of the porous support. The sampling swabs have flocked tips to ensure the microbial sampling volume. After a period of time in the groundwater simulation experimental column, the threaded cap is opened and the samples are taken out.

[0012] A method for simulating the impact of antibiotic input on groundwater denitrification and its microbial sampling, characterized in that: using the device for simulating the impact of antibiotic input on groundwater denitrification as described above, including the following steps:

[0013] First step, prepare the water body required for the experiment and inject it into the water supply water bottle for standby.

[0014] Second step, fill the groundwater simulation experimental column with a suitable medium, place the porous support and sampling swabs, vertically insert the pore water sampler, and seal the simulated groundwater sampling port.

[0015] Third step, connect the groundwater simulation experimental column to the water supply system and the water outlet water bottle to make the device in a sealed state.

[0016] Fourth step, pass pure water into the groundwater simulation experimental column through a peristaltic pump, calculate the porosity in the groundwater simulation experimental column, and calculate the required flow rate for the experiment.

[0017] Fifth step, simulate the groundwater denitrification system: pass the water body required for the experiment into the groundwater simulation experimental column through a peristaltic pump and continuously run for the required duration of the experiment.

[0018] Sixth step, testing of water samples, gas samples, and microbial samples: During the process of simulating the impact of antibiotics on groundwater denitrification, water samples can be taken out through the pore water sampler, gas samples can be collected through the aluminum foil sampling bag connected to the water outlet water bottle, and microbial samples can be taken out through the microbial sampling port; all sampling operations can be carried out at different time nodes, and then the required indicators of the water samples, gas samples, and microbial samples are determined.

[0019] Advantages and effects of the present invention:

[0020] First, the "Experimental Device for Stratified Monitoring of the Influence of Simulated Antibiotic Input on Groundwater Denitrification and Its Microbial Sampling Method" of the present invention realizes the simulation of the groundwater denitrification system; four groundwater simulation experimental columns are set as the experimental group, making the experimental results more accurate; the groundwater simulation experimental columns can take groundwater samples in layers;

[0021] Second, according to the actual situation and different pollution modes of antibiotics entering groundwater, various antibiotic input concentrations and frequencies can be set for the four groundwater simulation experimental columns;

[0022] Third, aiming at the difficulty of sampling groundwater microorganisms, a porous support is designed and placed inside the experimental column, which can sample microorganisms in layers during the experiment, and multiple sampling swabs are placed, greatly increasing the amount of microbial sampling;

[0023] Fourth, this device is placed on a support platform, with a compact and distinct structure. The four experimental columns can be removed and moved individually, and experimental factors such as flow rate and light can be controlled. Therefore, it has good popularization and application value. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Attached Figure 1 —— Schematic diagram of the structural principle of the present invention

[0025] Attached Figure 2 —— Schematic diagram of the porous support

[0026] Attached Figure 3 —— Schematic diagram of the operation of the device of the present invention

[0027] The corresponding part names for each label in the figure are as follows: 1 - water outlet, 2 - groundwater simulation experimental column, 3 - simulated groundwater sampling port, 4 - microbial sampling port, 5 - water inlet, 6 - porous support, 7 - small hole, 8 - water supply water bottle, 9 - peristaltic pump, 10 - medium, 11 - silica gel hose, 12 - water outlet water bottle, 13 - support platform. DETAILED DESCRIPTION OF THE INVENTION

[0028] The present invention provides an experimental device for stratified monitoring of the influence of simulated antibiotic input on groundwater denitrification and its microbial sampling method. This device sets four groundwater simulation experimental columns as the experimental group. The groundwater simulation experimental columns are all placed on a support platform. The groundwater simulation experimental columns are connected to a water supply water bottle through a silica gel hose and a peristaltic pump, and continuous water inlet is achieved by replacing the water supply water bottle; there are four vertical columns of sampling ports with different intervals on the side of the groundwater simulation experimental column. One column is the simulated groundwater sampling port, and three columns are microbial sampling ports. A pore water sampler is used to take water samples in layers at different heights of the simulated groundwater sampling ports in the groundwater simulation experimental column, and a porous support and sampling swabs are used to collect microbial samples. By analyzing these samples, the influence and mechanism of different antibiotic input modes on groundwater denitrification are further determined.

[0029] The present invention will be further described below in conjunction with the accompanying drawings.

[0030] As Figures 1 - 3 shown, an experimental device for layered monitoring of the impact of simulated antibiotic input on groundwater denitrification and its microbial sampling method of the present invention includes a groundwater simulation experimental column 2, a simulated groundwater sampling port 3, a microbial sampling port 4, a support platform 13, a porous support 6, a water supply water bottle 8, a peristaltic pump 9, a silica gel hose 11, and an effluent water bottle 12. The groundwater simulation experimental column 2 is placed on the support platform 13. The inlets 5 of the respective groundwater simulation experimental columns 2 are connected to the water supply water bottle 8 through the silica gel hose 11 and the peristaltic pump 9; the outlet 1 of the groundwater simulation experimental column 2 is connected to the effluent water bottle 12 through the silica gel hose 11; experimental water bodies can be injected into the column through the water supply water bottle 8 and the peristaltic pump 9, and run for a certain experimental period in this way to simulate the denitrification process in groundwater.

[0031] There are a total of four columns of vertically distributed sampling ports with different spacing distances on the side of the groundwater simulation experimental column 2, one of which is a simulated groundwater sampling port 3, and three are microbial sampling ports 4. Each microbial sampling port 4 is equipped with a threaded cap, and there is a cylinder in the center of the threaded cap, which can just block the vertical sampling port space.

[0032] In the present invention, the water supply system includes the inlets 5 of the respective groundwater simulation experimental columns 2, the silica gel hose 11, the water supply water bottle 8, and the peristaltic pump 9. Each experimental column is equipped with its own silica gel hose 11 and water supply water bottle 8, and water is introduced through the same peristaltic pump 9 to ensure the same water inlet rate. The water supply water bottle can be equipped with an air bag to balance the air pressure in the bottle.

[0033] In the present invention, the simulated groundwater sampling system includes a number of simulated groundwater sampling ports 3 and a pore water sampler. The pore water sampler is vertically inserted and placed in the simulated groundwater sampling port 3, and the simulated groundwater sampling port 3 can be sealed and wrapped with a sealing film.

[0034] In the present invention, the microbial sampling system includes a number of microbial sampling ports 4, a porous support 6, and sampling swabs. The porous support is composed of two circular plates with an outer diameter slightly smaller than the inner diameter of the column and a thickness of 3 mm. The circular plates are evenly distributed with small holes 7 with a diameter of 2 mm. The two circular plates are supported by four small cylinders to form a porous support. When the medium 10 in the experimental column is filled to the height of each microbial sampling port 4, the porous support 6 is placed. The vertical columnar sampling ports for microorganisms correspond to the hollow positions of the two circular plates of the porous support 6 one by one, and a nylon mesh or gauze is placed on the upper part of the porous support 6 to prevent the medium 10 from sinking due to gravity, and multiple sampling swabs are placed in the hollow position of the porous support. The sampling swabs are flocked heads to ensure the amount of microbial sampling, and the threaded cap is opened and taken out after the experimental column has run for a certain time.

[0035] In the present invention, a layered monitoring experimental device for simulating the influence of antibiotic input on groundwater denitrification and its microbial sampling method include the following steps;

[0036] In the first step, prepare the water body required for the experiment and inject it into the water supply water bottle 8 for standby;

[0037] In the second step, fill the groundwater simulation experimental column 2 with a suitable medium 10, place the porous support 6 and the sampling swab, insert the pore water sampler, and seal the groundwater sampling port.

[0038] In the third step, connect the groundwater simulation experimental column 2 with the water supply system and the water outlet water bottle 12 to make the device in a sealed state.

[0039] In the fourth step, introduce pure water into the groundwater simulation experimental column through a peristaltic pump, calculate the porosity in the groundwater simulation experimental column, and calculate the required flow rate for the experiment.

[0039] In the fifth step, simulate the groundwater denitrification system: introduce the water body required for the experiment into the column by the peristaltic pump 9 and continuously run for the required duration of the experiment.

[0040] In the sixth step, test the water samples, gas samples, and microbial samples: during the process of simulating the influence of antibiotics on groundwater denitrification, the water samples can be taken out through the pore water sampler, the gas samples can be collected through the aluminum foil sampling bag connected to the water outlet water bottle 12, and the microbial samples can be taken out through the microbial sampling port 4; all sampling operations can be carried out at different time nodes, and then the required indicators of the water samples, gas samples, and microbial samples are determined.

[0041] The above has described the specific implementation manners of the present invention in detail. In actual production and life, without departing from the principle of the present invention, those skilled in the art can make corresponding improvements, and these improvements are all regarded as the protection scope of the present invention.

Claims

1. A stratified monitoring experimental device for simulating the effect of antibiotic input on groundwater denitrification and a microbial sampling method thereof, characterized in that: The experimental device comprises a groundwater simulation experimental column (2), a water supply system, a simulated groundwater sampling system, a microorganism sampling system, and a supporting platform (13); The four groundwater simulation experiment columns (2) are all placed on a support platform (13). The water inlets (5) of the groundwater simulation experiment columns (2) are connected to the water supply bottle (8) through a silicone hose (11) and a peristaltic pump (9); the water outlets (1) of the groundwater simulation experiment columns (2) are connected to the water outlet bottle (12) through a silicone hose (11). The side of the groundwater simulation experiment column (2) has four rows of vertically distributed sampling ports with different spacing distances, one row of which is a simulated groundwater sampling port (3), and three rows are microbial sampling ports (4). Each microbial sampling port (4) is equipped with a threaded cover, and the center of the threaded cover has a cylinder that can just block the vertical sampling port space; The water supply system comprises a water inlet (5), a silicone hose (11), a water supply bottle (8) and a peristaltic pump (9) of each groundwater simulation experimental column (2). Each experimental column is equipped with its own silicone hose (11) and water supply bottle (8). Water is supplied through the same peristaltic pump (9) to ensure the same water supply rate. The water supply bottle (8) can be equipped with an air bag to balance the air pressure in the bottle. The simulated groundwater sampling system comprises a plurality of simulated groundwater sampling ports (3) and pore water samplers, wherein the pore water samplers are vertically inserted into the simulated groundwater sampling ports (3), and the simulated groundwater sampling ports (3) can be sealed and wrapped with sealing films; The microbial sampling system comprises a plurality of microbial sampling ports (4), a porous support (6) and a sampling swab. The porous support (6) is two circular plates with an outer diameter slightly smaller than the inner diameter of the groundwater simulation test column (2) and a thickness of 3 mm. Small holes (7) with a diameter of 2 mm are evenly distributed on the circular plates. The two circular plates are supported by four small cylinders to form a porous support (6). When the test column is filled with a medium (10) to the height of each microbial sampling port (4), the porous support (6) is placed. The microbial vertical column sampling port corresponds to the hollow positions of the two circular plates of the porous support (6) one by one. A nylon net or gauze is placed on the upper part of the porous support (6) to prevent the medium (10) from sinking due to gravity. A plurality of sampling swabs are placed in the hollow position of the porous support (6). The sampling swabs are flocked heads to ensure the microbial sampling amount. After the groundwater simulation test column (2) has been running for a period of time, the threaded cover is opened and taken out.

2. According to the experimental device for stratified monitoring of the effect of simulated antibiotic input on groundwater denitrification as described in claim 1, it is characterized by: The groundwater simulation experiment columns (2) are all placed on a supporting platform (13), and each experiment column is equipped with its own silicone hose (11) and water supply bottle (8), and water is supplied through the same peristaltic pump (9). The water outlets at the tops of the groundwater simulation experiment columns (2) are connected to the water outlet bottles (12) through the silicone hoses (11) to collect the water discharged from the groundwater simulation experiment columns (2).

3. A stratified monitoring and microbial sampling method for simulating the effect of antibiotic input on groundwater denitrification, characterized in that: The method comprises the following steps: The first step is to prepare the water required for the experiment and inject the water required for the experiment into the water supply bottle (8) for standby use; The second step is to fill the groundwater simulation test column (2) with a suitable medium, place a porous support (6) and a sampling swab, vertically insert the pore water sampler, and seal the simulated groundwater sampling port (3); The third step is to connect the groundwater simulation test column (2) to the water supply system and the water outlet bottle (12) so that the device is in a sealed state; The fourth step is to introduce pure water into the groundwater simulation experimental column through a peristaltic pump, calculate the porosity in the groundwater simulation experimental column, and calculate the flow rate required for the experiment; Step 5, simulating the groundwater denitrification system: the peristaltic pump (9) is used to introduce the water required for the experiment into the groundwater simulation experimental column (2), and the experiment is continuously operated for the required time; Step 6: Testing of water samples, gas samples and microbial samples: In the process of simulating the effect of antibiotics on groundwater denitrification, water samples can be taken out through a pore water sampler, gas samples can be collected through an aluminum foil sampling bag connected to a water outlet bottle (12), and microbial samples can be taken out through a microbial sampling port (4); all sampling operations can be performed at different time points, and then the water samples, gas samples and microbial samples are tested for the required indicators.

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