Reaction device and method for sequentially reacting reagents

Abstract

The embodiment of the invention belongs to the technical field of biological detection, and relates to a reaction device and a reagent sequential reaction method, the reaction device comprises a main body and a reaction chamber, the reaction chamber comprises a reaction cavity, and the reaction cavity is used for reagent reaction; the plurality of reagent storage chambers are arranged on the main body and are provided with reagent storage cavities communicated with the reaction cavity and the external environment; the driving part is movably connected to the reaction chamber and located in the reaction cavity, the shape and size of the driving part are matched with those of the reaction cavity, and the driving part is used for moving in the reaction cavity and generating air pressure so as to drive liquid in the reagent storage cavity to flow into or out of the reaction cavity; and the unlocking piece is connected to the main body and is used for controlling the plurality of reagent storage cavities to be communicated with or blocked from the external environment in sequence so as to load the reagents in sequence. By controlling the unlocking piece, communication and blocking of the reagent storage cavity can be controlled, then by means of pressure generated by movement of the driving piece, reagents are sequentially introduced into the reaction cavity for reaction, no valve needs to be additionally arranged, and cost is reduced.

Description

Technical Field

[0001] The present application relates to the field of biological detection technologies, and particularly relates to a reaction device and a method for sequential reaction of reagents. Background Art

[0002] When performing in vitro diagnostic tests, it is usually necessary to use a reaction device to mix reagents, so as to obtain a final diagnostic result through reactions between various reagents. For example, when performing nucleic acid purification in molecular diagnosis, it is necessary to contact and react various reagents with the analyte in a certain order. The reaction device usually includes a microfluidic chip, which is similar to a chemical and biological laboratory integrating biochemical reactions on a small chip, and can integrate some reaction steps required in chemical and biological reactions, such as reaction, culture, separation, sorting, and detection, etc. on a microchip. Structurally, the reaction device generally consists of a reagent storage chamber, a microchannel, a first air vent, and a reaction and detection chamber.

[0003] When it is necessary to load reagents in a certain order, it is mainly achieved by valves in the microfluidic chip, such as a rotary valve. When a power source is applied to the fluid, the reagents will sequentially pass through different valves, thereby achieving sequential loading of the reagents. However, adding an external valve to the microfluidic chip will increase its cost, and will result in poor yield and reliability of the microfluidic chip. Among them, the rotary valve requires precise manufacturing to ensure its flatness and roundness, resulting in a low yield. At the same time, the reaction device will generate waste liquid after reacting the analyte and reagents. In the reaction device of the prior art, a waste liquid pool needs to be provided to accommodate the waste liquid. In summary, the reaction device in the prior art needs to add structures such as valves and waste liquid pools, resulting in high costs, low space utilization, and low portability. Summary of the Invention

[0004] The technical problem to be solved by the embodiments of the present application is the problem that the existing reaction device has high costs, low space utilization, and low portability.

[0005] To solve the above technical problems, the embodiments of the present application adopt the following solutions:

[0006] A reaction device, comprising:

[0007] A main body;

[0008] A reaction chamber, arranged in the main body, the reaction chamber includes a reaction cavity communicating with the external environment, and the reaction cavity is used for the reaction of the reagents;

[0009] A plurality of reagent storage chambers, the reagent storage chambers are arranged in the main body, and are provided with reagent storage cavities communicating with the reaction cavity and the external environment, and the reagent storage cavities are used for accommodating the reagents;

[0010] A driving member, which is movably connected to the reaction chamber, located in the reaction cavity, and is adapted in shape and size to the reaction cavity, for moving in the reaction cavity and generating air pressure in the reaction cavity to drive the liquid in the reagent storage cavity to flow into or out of the reaction cavity;

[0011] An unlocking member, which is connected to the main body and is used to control a plurality of the reagent storage cavities to communicate with or be blocked from the external environment in sequence, so as to load reagents in sequence.

[0012] Further, the main body is provided with a plurality of first air-permeable holes, and each of the reagent storage cavities communicates with the external environment through one of the first air-permeable holes;

[0013] The unlocking member can move on the main body to control the plurality of first air-permeable holes to communicate with or be blocked in sequence.

[0014] Further, the main body is provided with an unlocking groove, and at least one end of the unlocking groove in its length direction penetrates the main body;

[0015] A plurality of the first air-permeable holes are all arranged in the unlocking groove and are distributed along the length direction of the unlocking groove;

[0016] The unlocking member is a slider, which is arranged in the unlocking groove and can reciprocate along the length direction of the unlocking groove to make the first air-permeable holes communicate with or be blocked.

[0017] Further, the unlocking member is provided with a plurality of second air-permeable holes, and the diameter of the second air-permeable holes is smaller than the distance between adjacent first air-permeable holes. When the second air-permeable holes communicate with the first air-permeable holes, the corresponding reagent storage cavity communicates with the external environment.

[0018] Further, the first air-permeable holes are provided with hydrophobic breathable membranes.

[0019] Further, the inner side wall of the unlocking groove is provided with a fixing groove;

[0020] A fixing protrusion is arranged on the part of the unlocking member facing the inner side wall of the unlocking groove, and the fixing protrusion is located in the fixing groove and cooperates with the fixing groove to limit the unlocking member to move only along the length direction of the unlocking groove.

[0021] Further, the main body is provided with a plurality of communication grooves, and both ends of the communication grooves communicate with the reaction cavity and one of the reagent storage cavities respectively;

[0022] The communication groove includes a flow-through part and a buffer part. The buffer part is located between the reaction cavity and the reagent storage cavity, and the flow area of the buffer part is larger than the flow area of the flow-through part.

[0023] Further, the reaction device further includes a sealing cover, and the reagent storage chamber includes a first reagent storage chamber and a second reagent storage chamber;

[0024] A part of the first reagent storage chamber extends beyond the main body and is provided with a sealing channel. The sealing channel communicates the reagent storage cavity and the external environment. The sealing cover is sleeved on the part of the first reagent storage chamber that extends beyond the main body and seals the sealing channel.

[0025] Further, the reaction device further includes a sealing plate. The reagent storage chamber and the reaction chamber arch away from the main body to respectively form a reagent storage cavity and a reaction cavity. The sealing plate is connected to one side of the main body opposite to the reagent storage chamber and the reaction chamber for sealing the reagent storage cavity and the reaction cavity; and / or

[0026] The driving member includes a piston portion and a piston rod connected to each other. The piston portion is located in the reaction cavity, and the length of the piston rod is greater than the length of the reaction cavity.

[0027] Correspondingly, the present application also provides a method for sequential reaction of reagents, which is applied to the sequential reaction device according to any one of the embodiments. The method includes:

[0028] A. Move the unlocking member to communicate the reagent storage cavity storing the reagent with the external environment;

[0029] B. Drive the driving member in a direction away from the reaction cavity to control the reagent in the reagent storage cavity communicated with the external environment to flow into the reaction cavity;

[0030] C. Repeat steps A and B to enable the reagents in all reagent storage cavities to flow into the reaction cavity in sequence for reaction;

[0031] D. Drive the driving member in the direction of the reaction cavity to enable the mixed reagent to return to the reagent storage cavity.

[0032] Compared with the prior art, the embodiments of the present application mainly have the following beneficial effects:

[0033] The present application can control the movement of the unlocking member to communicate a part of the reagent storage cavity with the external environment, and then control the movement of the driving member in the reaction cavity to realize the inflow or outflow of the reagent into or out of the reaction cavity. By controlling the unlocking member, the present application can sequentially communicate the reagent storage cavities, and thus can add the reagents into the reaction cavity in sequence for reaction without additionally adding devices such as "valves". Therefore, the present application reduces the production cost of the reaction device. At the same time, the reagent storage cavity undertakes the function of accommodating the mixed reagent, and there is no need to set up a waste liquid pool, reducing the volume of the reaction device. Description of the Drawings

[0034] To more clearly illustrate the solutions in the present application or the prior art, the following will give a brief introduction to the drawings required for the description of the embodiments or the prior art. Obviously, the drawings in the following description are some embodiments of the present application. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0035] Figure 1 is a schematic structural diagram of the reaction device according to an embodiment of the present application;

[0036] Figure 2 is another schematic structural diagram of the reaction device according to an embodiment of the present application;

[0037] Figure 3 is another schematic structural diagram of the reaction device according to an embodiment of the present application.

[0038] Reference numerals:

[0039] reaction chamber 10, reagent storage chamber 20, first reagent storage chamber 21, second reagent storage chamber 22, seal 30, hydrophobic breathable membrane 40, main body 100, first ventilation hole 110, first sub-reagent storage cavity 121, second sub-reagent storage cavity 122, third sub-reagent storage cavity 123, fourth sub-reagent storage cavity 124, fifth sub-reagent storage cavity 125, communication groove 130, buffer portion 131, circulation portion 132, unlocking groove 140, driving member 200, piston portion 210, piston rod 220, unlocking member 300, second ventilation hole 310, sealing cover 400, sealing plate 500. Detailed implementation manners

[0040] The following will clearly and completely describe the technical solutions in the embodiments of the present application with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only some embodiments of the present application, rather than all embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative efforts belong to the scope of protection of the present application. In addition, it should be understood that the specific implementation manners described herein are only used to illustrate and explain the present application, and are not used to limit the present application.

[0041] In the present application, unless otherwise stated, the orientation terms such as "upper" and "lower" generally refer to the upper and lower in the actual use or working state of the device, specifically the drawing direction in the drawings; and "inner" and "outer" refer to the outline of the device. In addition, in the description of the present application, the term "including" means "including but not limited to". The terms first, second, third, etc. are only used as labels and do not impose numerical requirements or establish an order.

[0042] In this application, "and / or" describes the association relationship of associated objects, indicating that there can be three relationships. For example, A and / or B can represent: A exists alone, A and B exist simultaneously, or B exists alone. Here, A and B can be singular or plural.

[0043] In this application, "at least one" means one or more, and "a plurality" means two or more. "At least one kind", "at least one of the following (items)" or similar expressions refer to any combination of these items, including any combination of single item(s) or plural item(s). For example, "at least one of a, b, or c", or "at least one of a, b, and c" can both represent: a, b, c, a - b (i.e., a and b), a - c, b - c, or a - b - c, where a, b, and c can be single or plural respectively.

[0044] The various embodiments of this application may exist in the form of a range; it should be understood that the description in the form of a range is only for convenience and brevity, and should not be construed as a rigid limitation on the scope of this application; therefore, it should be considered that the range description has specifically disclosed all possible sub - ranges and single values within that range. For example, it should be considered that the range description from 1 to 6 has specifically disclosed sub - ranges such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6, etc., and single numbers within the range, such as 1, 2, 3, 4, 5, and 6, and this applies regardless of the range. Additionally, whenever a numerical range is indicated herein, it means including any cited number (fraction or integer) within the indicated range. The straight line with an arrow in the figure is a schematic line of the emission line of thermal radiation.

[0045] Please refer to Figures 1 to 3 , an embodiment of this application provides a reaction device, including a main body 100, a driving member 200, an unlocking member 300, a reaction chamber 10, and a plurality of reagent storage chambers 20, where: The reaction chamber 10 is arranged in the main body 100, and the reaction chamber 10 includes a reaction cavity (not shown in the figure) communicating with the external environment, and the reaction cavity is used for reagent reaction; The reagent storage chamber 20 is arranged in the main body 100 and is provided with a reagent storage cavity communicating with the reaction cavity and the external environment, and the reagent storage cavity is used for accommodating reagents; The driving member 200 is movably connected to the reaction chamber 10, located in the reaction cavity, and its shape and size are adapted to the reaction cavity, and is used for moving in the reaction cavity and generating air pressure in the reaction cavity to drive the liquid in the reagent storage cavity to flow into or out of the reaction chamber; The unlocking member 300 is connected to the main body 100 and is used to control the plurality of reagent storage cavities to be sequentially communicated with or blocked from the external environment to load reagents in sequence.

[0046] In this embodiment, when the unlocking member 300 controls a certain reagent storage cavity to be in a closed state, since the driving member 200 is located in the reaction cavity, the space formed by the reagent storage cavity and the reaction cavity is in a closed environment at this time. Under the action of air pressure, the liquid in the reagent storage cavity cannot flow into the reaction cavity from the reagent storage cavity. When the unlocking member 300 controls a certain reagent storage cavity to communicate with the external environment, controlling the movement of the driving member 200 at this time will cause a negative pressure or a positive pressure in the reaction cavity. When the driving member 200 moves in a direction away from the reaction cavity, the space formed by the reaction cavity and the reagent storage cavity becomes larger, and its pressure decreases. The external atmospheric pressure will drive the reagent in the reagent storage cavity to move into the reaction cavity.

[0047] Therefore, the present application can control the movement of the unlocking member 300 to make a part of the reagent storage cavity communicate with the external environment, and then control the driving member 200 to move in a direction away from the reaction cavity, thereby generating a negative pressure in the reaction cavity. At this time, due to the inflow of external gas, the reagent stored in the reagent storage cavity that communicates with the external environment will flow into the reaction cavity. After the reagent reaction is completed, the driving member 200 can also be controlled to move in the direction of the reaction cavity, so that the mixed reagent in the reaction cavity moves into the reagent storage cavity that communicates with the external environment.

[0048] By controlling the unlocking member 300, this embodiment can sequentially connect the reagent storage cavities, so that it is possible to sequentially add reagents into the reaction cavity for reaction without additionally adding devices such as "valves". Therefore, the present application reduces the production cost of the reaction device. At the same time, the reagent storage cavity serves to accommodate the mixed reagent, eliminating the need to set up a waste liquid pool and reducing the volume of the reaction device.

[0049] It can be understood that the shape and size of the driving member 200 being adapted to the reaction cavity should be understood as: the driving member 200 can fill the reaction cavity. When the driving member 200 blocks the reaction cavity, the reaction cavity is only connected to the reagent storage cavity and cannot communicate with the external environment. The connection between the reagent storage chamber 20 and the reaction cavity can be achieved by structures such as opening through holes on the main body 100 and adding pipes.

[0050] Furthermore, please refer to Figures 1 to 3 , the main body 100 is provided with a plurality of first air vents 110, and each reagent storage cavity communicates with the external environment through a first air vent 110; the unlocking member 300 can move on the main body 100 to control the plurality of first air vents 110 to be sequentially connected or blocked.

[0051] In this embodiment, the unlocking member 300 can be structures such as a sealing plug or a sealing clip. However, if the unlocking member 300 is a structure such as a sealing plug or a sealing clip, when the unlocking member 300 controls the occlusion of the reagent storage cavity or its communication with the external environment, a pre-tightening force will inevitably be generated. This pre-tightening force may cause the deformation of the first ventilation hole 110 or the clamping deformation of the main body 100, thereby reducing the service life of the reaction device. Moreover, since the first ventilation hole 110 is usually small to prevent the reagent stored in the reagent storage cavity from flowing out, if the unlocking member 300 is a sealing plug at this time, the volume of the unlocking member 300 is small, which is not conducive to grasping, resulting in low operation efficiency of the reaction device.

[0052] In summary, the unlocking member 300 of this embodiment adopts a moving manner on the main body 100, and the unlocking member 300 itself is used to block the first ventilation hole 110, thereby realizing the control of the occlusion of the reagent storage cavity or its communication with the external environment. This structure will not cause the deformation of the first ventilation hole 110 and the main body 100, and can also ensure that the volume of the unlocking member 300 is sufficient for grasping. In summary, this embodiment can improve the service life and operation efficiency of the reaction device.

[0053] Further, please refer to Figures 1 to 3 , the main body 100 is provided with an unlocking groove 140, and at least one end of the unlocking groove 140 in its length direction penetrates the main body 100; a plurality of first ventilation holes 110 are all arranged in the unlocking groove 140 and are distributed along the length direction of the unlocking groove 140; the unlocking member 300 is a slider arranged in the unlocking groove 140 and can reciprocate along the length direction of the unlocking groove 140 to connect or block the first ventilation holes 110.

[0054] It can be understood that there can be multiple unlocking members 300, and the multiple unlocking members 300 can all slide independently on the unlocking groove 140, thereby realizing the independent control of multiple reagent storage cavities. A sealing member 30 can also be included between the unlocking member 300 and the unlocking groove 140 or the waterproof breathable film 40, so as to prevent liquid from leaking from the sealing groove 140 through the sealing member 30. The sealing member 30 can be structures such as a sealing film, double-sided tape or plastic plate.

[0055] Further, please refer to Figures 1 to 3 , the main body 100 is provided with an unlocking groove 140, and at least one end of the unlocking groove 140 in its length direction penetrates the main body 100; a plurality of first ventilation holes 110 are all arranged in the unlocking groove 140 and are distributed along the length direction of the unlocking groove 140; the unlocking member 300 is a slider arranged in the unlocking groove 140 and can reciprocate along the length direction of the unlocking groove 140 to connect or block the first ventilation holes 110.

[0056] Further, please refer to Figures 1 to 3, the unlocking member 300 is provided with a plurality of second ventilation holes 310, the diameter of the second ventilation holes 310 is A, the distance between adjacent first ventilation holes 110 is B, and B is greater than A. When the second ventilation holes 310 are used to communicate with the first ventilation holes 110, the reagent storage cavity communicates with the external environment.

[0057] When using the unlocking member 300 to control the communication and occlusion of the first ventilation holes 110, if it is necessary to simultaneously control two or more non-adjacent first ventilation holes 110 to be in a communicating state, it is usually necessary to use two independent unlocking members 300 to achieve. And in this application, by providing a plurality of second ventilation holes 310, it is thus possible to achieve the communication state of multiple reagent storage cavities that originally required multiple independent unlocking members 300 with only one unlocking member 300, reducing the operation difficulty of the reaction device. At the same time, if the unlocking member 300 is not provided with a plurality of second ventilation holes 310, it is necessary to move the unlocking member 300 a relatively long distance so that the unlocking member 300 no longer covers the first ventilation holes 110 in order to make two first ventilation holes 110 in a communicating or occluding state. However, because the unlocking member 300 is provided with a plurality of second ventilation holes 310, it is only necessary to move a relatively short distance, and only by making the second ventilation holes 310 communicate with the first ventilation holes 110 can two first ventilation holes 110 be in a communicating or occluding state. In summary, this embodiment can improve the operation efficiency of the reaction device.

[0058] Further, please refer to Figure 3 , a hydrophobic breathable membrane 40 is provided in the first ventilation hole 110. During the actual working process, when it is necessary to push the mixed reagent in the reaction cavity back into the reagent storage cavity, it may occur that the reagent flows out from the first ventilation hole 110 or the reagent leaks from the first ventilation hole 110 during transportation, which may lead to environmental pollution. To avoid this situation, in this embodiment, a hydrophobic breathable membrane 40 is provided at the first ventilation hole 110, and this hydrophobic breathable membrane 40 only allows gas to pass through, so it can prevent the mixed reagent from overflowing from the first ventilation hole 110, thereby avoiding reagent pollution of the environment.

[0059] It is understandable that the material of the hydrophobic and breathable membrane 40 includes ePTFE. The installation methods of the hydrophobic and breathable membrane 40 include methods such as double-sided tape, ultrasonic welding, and laser welding. The pore size range of the hydrophobic and breathable membrane 40 is 0.01μm to 50μm. Within this range, the hydrophobic and breathable membrane 40 can ensure a good isolation effect on liquids while improving the gas permeability. The pore size range of the hydrophobic and breathable membrane 40 can be any value between 0.01μm, 1μm, 2μm, 3μm, 4μm, 5μm, 6μm, 7μm, 8μm, 9μm, 10μm, 11μm, 12μm, 13μm, 14μm, 15μm, 16μm, 17μm, 18μm, 19μm, 20μm, 21μm, 22μm, 23μm, 24μm, 25μm, 26μm, 27μm, 28μm, 29μm, 30μm, 31μm, 32μm, 33μm, 34μm, 35μm, 36μm, 37μm, 38μm, 39μm, 40μm, 41μm, 42μm, 43μm, 44μm, 45μm, 46μm, 47μm, 48μm, 49μm, 50μm or the range formed by any two values.

[0060] Further, please refer to Figure 3 , a fixing groove is provided on the inner side wall of the unlocking groove 140; a fixing protrusion is provided on the unlocking member 300 at the part facing the inner side wall of the unlocking groove 140. The fixing protrusion is located in the fixing groove and cooperates with the fixing groove to limit the unlocking member 300 to move only along the length direction of the unlocking groove 140.

[0061] To prevent the unlocking member 300 from falling off from the unlocking groove 140, in this embodiment, through the clamping connection of the fixing protrusion and the fixing groove, the unlocking member 300 can be restricted in the unlocking groove 140, and only allows the unlocking member 300 to reciprocate along the Y direction in the figure.

[0062] Further, please refer to Figure 1 , the main body 100 is provided with a plurality of communication grooves 130. The two ends of the communication grooves 130 are respectively communicated with the reaction chamber and a reagent storage chamber; the communication grooves 130 include a flow-through part 132 and a buffer part 131. The buffer part 131 is located between the reaction chamber and the reagent storage chamber, and the flow area of the buffer part 131 is larger than the flow area of the flow-through part 132.

[0063] The flow area should be understood as the area through which the reagent flows when passing through this part, or it can also be understood as the groove diameter of the communication groove 130. During the use of the reagent, due to insufficient airtightness of the reaction device, the reagent in the reagent storage cavity may still flow into the reaction cavity when it is stationary. In this case, it is easy to cause the mixed reagent to not match the expected type, thereby resulting in a diagnostic error. Therefore, in this embodiment, a buffer portion 131 is provided, and the flow area of the buffer portion 131 is larger than that of the flow-through portion 132. When the reagent flows from the flow-through portion 132 to the buffer portion 131, it needs to fill the buffer portion 131 before it can continue to flow into the reaction cavity, thereby avoiding the reagent flowing into the reaction cavity before the reaction and affecting the diagnostic result.

[0064] It can be understood that the flow-through portion 132 can also be bent and arranged on the main body 100, thereby increasing the resistance of the reagent during flow through multiple bends, and further avoiding the reagent flowing directly into the reaction cavity before the reaction.

[0065] Furthermore, please refer to Figures 1 to 3 , the reaction device further includes a sealing cover 400, and the reagent storage chamber 20 includes a first reagent storage chamber 21 and a second reagent storage chamber 22; a part of the first reagent storage chamber 21 extends beyond the main body 100 and is provided with a sealing channel, and the sealing channel communicates the reagent storage cavity with the external environment. The sealing cover 400 is sleeved on the part of the first reagent storage chamber 21 that extends beyond the main body 100 and the sealing channel.

[0066] During the actual working process, in addition to storing the reagent for detection, the reagent storage chamber 20 also needs to be convenient for the user to add the object to be detected. Therefore, in this embodiment, the reagent storage chamber 20 is divided into a first reagent storage chamber 21 and a second reagent storage chamber 22, and a part of the first reagent storage chamber 21 extends beyond the main body 100, thereby facilitating the setting of the sealing cover 400 to seal the first reagent storage cavity through the sealing cover 400. It should be noted that there can be multiple second reagent storage chambers 22, and the capacities of the second reagent storage chambers 22 are different. For example Figure 3 the two second reagent storage chambers 22 marked in

[0067] have different heights protruding from the main body 100, and accordingly, the reagent capacities that can be loaded are different. When it is necessary to add the object to be detected, the sealing cover 400 can be opened, and then the object to be detected can be added to the first reagent storage cavity. When preparing to pump the object to be detected in the first reagent storage cavity into the reaction cavity, the first air-permeable hole 110 corresponding to the first reagent storage cavity can be opened, or the sealing channel can be kept communicating with the external environment.

[0068] Furthermore, please refer to Figure 3, the reaction device further includes a sealing plate 500. The reagent storage chamber 20 and the reaction chamber 10 arch away from the main body 100 to form a reagent storage cavity and a reaction cavity respectively. The sealing plate 500 is connected to one side of the main body 100 opposite to the reagent storage chamber 20 and the reaction chamber 10 for sealing the reagent storage cavity and the reaction cavity.

[0069] If the reagent storage chamber 20 and the reaction chamber 10 are connected to the main body 100 in a connected manner, during the process of assembling the reaction device, multiple assembling processes are required, which results in low production efficiency of the reaction device. In this embodiment, the reagent storage chamber 20, the reaction chamber 10 and the main body 100 are integrally formed. The reagent storage cavity and the reaction cavity are formed by arching, and then the above cavities are sealed by a sealing plate 500. In the assembling process, the sealing plate 500 needs to be connected to the main body 100. Therefore, this embodiment can effectively improve the installation efficiency of the reaction device. It can be understood that when the reaction device further includes a flow channel 130, the sealing plate 500 seals the flow channel 130 at the same time. When the reaction chamber 10 or the reagent storage chamber 20 in the reaction device is not formed by arching of the main body 100, the sealing plate 500 may not seal the reaction cavity or the reagent storage cavity formed by this structure.

[0070] Further, please refer to Figures 1 to 3 , the reaction chamber 10 can be located in the middle of the main body 100, and the reagent storage chambers 20 are evenly and symmetrically distributed on both sides of the reaction chamber 10. This design can avoid the reaction device from being deformed due to large unilateral force, and at the same time, it is also convenient for the user to grasp stably.

[0071] Further, please refer to Figures 1 to 3 , the driving member 200 includes a piston portion 210 and a piston rod 220 connected to each other. The piston portion 210 is located in the reaction cavity, and the length of the piston rod 220 is greater than the length of the reaction cavity.

[0072] At this time, the piston rod 220 can be conveniently grasped to control the movement of the piston portion 210 in the reaction cavity, so as to realize the control of the reagent. It can be understood that the piston portion 210 can be an elastic member, and it is in interference fit with the reaction cavity, thereby improving the airtightness of the reaction device.

[0073] Correspondingly, this embodiment provides a method for sequential reaction of reagents, which is applied to the sequential reaction device of any one of the above embodiments. The method includes:

[0074] A. Move the unlocking member 300 to communicate the reagent storage cavity storing the reagent with the external environment;

[0075] B. Drive the driving member 200 in a direction away from the reaction cavity to control the reagent in the reagent storage cavity communicated with the external environment to flow into the reaction cavity;

[0076] C. Repeat steps A and B to enable the reagents in all reagent storage chambers to flow into the reaction chamber in sequence for reaction;

[0077] D. Drive the driving member 200 in the direction towards the reaction chamber to cause the mixed reagent to return to the reagent storage chamber.

[0078] Through the above method, in this embodiment, various reagents can be sequentially added to the reaction chamber for reaction, realizing the sequential loading of reagents. It can be understood that during the process of loading reagents in this embodiment, multiple reagents in multiple reagent storage chambers can be loaded at one time, or only the reagents in one reagent storage chamber can be loaded. At the same time, step D of this application can be executed once after each execution of step B, or only executed once after the execution of step C.

[0079] It can be understood that since the produced reaction device may already store reagents in the reagent storage chamber, or does not store reagents in the reagent storage chamber and needs to add reagents according to actual requirements subsequently. Therefore, during the process of realizing the sequential reaction of reagents using the above method, the following steps can also be carried out before step A: adding multiple reagents to multiple reagent storage chambers respectively, or confirming that each reagent storage chamber already stores each reagent. After the reagents react in the reaction chamber, the reaction mixture can be retracted into each reagent storage chamber by controlling the unlocking member 300 and the driving member 200, thereby avoiding insufficient space in the reaction chamber.

[0080] When this method is used for nucleic acid purification, this application has six reagent storage chambers, including five second reagent storage chambers and one first reagent storage chamber (located in the first reagent storage room 21). The five second reagent storage chambers are respectively: the first sub-reagent storage chamber 121, the second sub-reagent storage chamber 122, the third sub-reagent storage chamber 123, the fourth sub-reagent storage chamber 124, and the fifth sub-reagent storage chamber 125. Among them, lysis buffer is pre-encapsulated in the first reagent storage chamber; proteinase K solution is pre-encapsulated in the first sub-reagent storage chamber 121; magnetic bead solution is pre-encapsulated in the second sub-reagent storage chamber 122; the first washing solution is pre-encapsulated in the third sub-reagent storage chamber 123; the second washing solution is pre-encapsulated in the fourth sub-reagent storage chamber 124; elution buffer is pre-encapsulated in the fifth sub-reagent storage chamber 125; lysis buffer is pre-encapsulated in the first reagent storage chamber. In the initial state, each reagent storage chamber is in a closed state.

[0081] The method for nucleic acid purification is as follows:

[0082] Add the sample to be detected into the first reagent storage chamber to mix the sample to be detected with the lysis buffer to form a first mixture;

[0083] Control the driving member 200 to move in the direction away from the reaction chamber, and a negative pressure is generated in the reaction chamber, so that the first mixture in the first reagent storage chamber flows into the reaction chamber;

[0084] Control the unlocking member 300 to communicate the first sub-reagent storage chamber 121 with the outside;

[0085] Control the driving member 200 to move away from the reaction chamber, creating a negative pressure in the reaction chamber, so that the proteinase K solution in the first sub-reagent storage chamber 121 flows into the reaction chamber, causing the first mixture to mix with the proteinase K solution;

[0086] Control the unlocking member 300 to occlude the first sub-reagent storage chamber 121;

[0087] Control the temperature of the main chamber to rise, causing the first mixture and the proteinase K solution in the reaction chamber to lyse and form a second mixture;

[0088] Control the driving member 200 to move towards the inside of the reaction chamber, causing the second mixture to retreat into the first reagent storage chamber, and control the unlocking member 300 to occlude the first reagent storage chamber;

[0089] Control the unlocking member 300 to communicate the second sub-reagent storage chamber 122 with the outside;

[0090] Control the driving member 200 to move away from the reaction chamber, creating a negative pressure in the reaction chamber, so that the magnetic bead solution in the second sub-reagent storage chamber 122 flows into the reaction chamber;

[0091] Attach a magnet to the outer wall of the reaction chamber 10, causing the magnetic beads in the magnetic bead solution to adsorb to the inner wall of the reaction chamber;

[0092] Control the driving member 200 to move towards the inside of the reaction chamber, causing the magnetic bead solution without magnetic beads to retreat into the second sub-reagent storage chamber 122, and control the unlocking member 300 to occlude the second sub-reagent storage chamber 122;

[0093] Control the unlocking member 300 to communicate the first reagent storage chamber with the outside;

[0094] Control the driving member 200 to move away from the reaction chamber, causing the second mixture to flow into the reaction chamber, and remove the magnet on the outer wall of the reaction chamber 10, so that the second mixture is fully mixed with the magnetic beads;

[0095] Attach the magnet to the outer wall of the reaction chamber 10;

[0096] Control the driving member 200 to move towards the inside of the reaction chamber, causing the second mixture to retreat into the first reagent storage chamber, and control the unlocking member 300 to occlude the first reagent storage chamber;

[0097] Control the unlocking member 300 to communicate the third sub-reagent storage chamber 123 with the external environment, and then control the driving member 200 to move away from the reaction chamber, so that the first washing liquid washes the magnetic beads;

[0098] Control the driving member 200 to move towards the reaction chamber, so that the first washing liquid retreats to the third sub-reagent storage chamber 123, and control the unlocking member 300 to block the third sub-reagent storage chamber 123;

[0099] Control the unlocking member 300 to communicate the fourth sub-reagent storage chamber 124 with the external environment, and then control the driving member 200 to move away from the reaction chamber, so that the second washing liquid washes the magnetic beads;

[0100] Control the driving member 200 to move towards the reaction chamber, so that the mixed liquid of the second washing liquid retreats to the fourth sub-reagent storage chamber 124, and control the unlocking member 300 to block the fourth sub-reagent storage chamber 124;

[0101] Control the unlocking member 300 to communicate the fifth sub-reagent storage chamber 125 with the external environment, and then control the driving member 200 to move away from the reaction chamber, so that the eluent enters the reaction chamber for elution;

[0102] Control the driving member 200 to move towards the reaction chamber, so that the eluent in the reaction chamber returns to the fifth sub-reagent storage chamber 125, and the nucleic acid purification solution in the fifth sub-reagent storage chamber 125 is obtained.

[0103] It can be understood that the above-mentioned method of fully mixing the second mixed liquid with the magnetic beads includes repeatedly moving the driving member 200, so that the second mixed liquid in the reaction chamber repeatedly flushes the magnetic beads; ultrasonic oscillation, vortex oscillation, that is, shaking and mixing, etc. When the reaction chamber is heated, its temperature can be 70 °C.

[0104] Furthermore, since the driving member 200 can move along the length direction of the reaction chamber, if the magnetic beads are adsorbed to the side wall of the reaction chamber under the action of the magnet, the magnetic beads may be pushed away from the preset position by the driving member 200, resulting in the loss of the adsorption effect of the magnetic beads, and further reducing the nucleic acid purification effect. Preferably, along the length direction of the reaction chamber, the reaction chamber has a bottom wall; the magnet is adsorbed to the bottom wall of the reaction chamber, and the magnetic beads in the magnetic bead liquid are adsorbed to the bottom wall of the reaction chamber. At this time, during the movement of the driving member 200, the magnetic beads in this embodiment are carried by the bottom wall, and the displacement of the magnetic beads caused by the pushing of the driving member 200 can be avoided, thereby improving the nucleic acid purification effect

[0105] Obviously, the embodiments described above are only a part of the embodiments of this application, rather than all embodiments. The preferred embodiments of this application are shown in the drawings, but do not limit the patent scope of this application. This application can be implemented in many different forms. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosed content of this application more thorough and comprehensive. Although this application has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions described in the foregoing specific embodiments, or perform equivalent replacements on some of the technical features. Any equivalent structures made by using the content of this application's specification and drawings, directly or indirectly applied in other related technical fields, are equally within the scope of patent protection of this application.

[0106] Although the embodiments of this application have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, combinations, replacements, and variations can be made to these embodiments without departing from the principles and purposes of this application. The scope of the present invention is defined by the claims and their equivalents.

Claims

1. A reaction device, characterized in that, Comprising: A main body; A reaction chamber, disposed in the main body, the reaction chamber including a reaction cavity communicating with the external environment, the reaction cavity being for reagent reaction; A plurality of reagent storage chambers, the reagent storage chambers being disposed in the main body and having reagent storage cavities communicating with the reaction cavity and the external environment, the reagent storage cavities being for accommodating the reagents; A driving member, movably connected to the reaction chamber, located in the reaction cavity, having a shape and size adapted to the reaction cavity, for moving in the reaction cavity and generating air pressure in the reaction cavity to drive the liquid in the reagent storage cavity to flow into or out of the reaction cavity; An unlocking member, connected to the main body, for controlling the plurality of reagent storage cavities to communicate with or be blocked from the external environment in sequence to load reagents in sequence.

2. The reaction device according to claim 1, characterized in that, The main body is provided with a plurality of first air-permeable holes, and each reagent storage cavity communicates with the external environment through one of the first air-permeable holes; The unlocking member is movable on the main body to control the plurality of first air-permeable holes to communicate with or be blocked from each other in sequence.

3. The reaction device according to claim 2, characterized in that, The main body is provided with an unlocking groove, at least one end of the unlocking groove in its length direction penetrating through the main body; The plurality of first air-permeable holes are all disposed in the unlocking groove and are distributed along the length direction of the unlocking groove; The unlocking member is a slider, disposed in the unlocking groove, capable of reciprocating along the length direction of the unlocking groove to make the first air-permeable holes communicate with or be blocked from each other.

4. The reaction device according to claim 3, characterized in that, The unlocking member is provided with a plurality of second air-permeable holes, the diameter of the second air-permeable holes being smaller than the distance between adjacent first air-permeable holes, and when the second air-permeable holes communicate with the first air-permeable holes, the corresponding reagent storage cavity communicates with the external environment.

5. The reaction device according to claim 3, characterized in that, The inner side wall of the unlocking groove is provided with a fixing groove; A fixing protrusion is provided on the unlocking member at a position facing the inner side wall of the unlocking groove, the fixing protrusion being located in the fixing groove and cooperating with the fixing groove to limit the unlocking member to move only along the length direction of the unlocking groove.

6. The reaction device according to claim 2, characterized in that, The first air-permeable holes are provided with hydrophobic air-permeable membranes.

7. The reaction device according to claim 2, characterized in that, The main body is provided with a plurality of communication grooves, both ends of the communication grooves communicating with the reaction cavity and one of the reagent storage cavities respectively; The communication grooves include a flow-through portion and a buffer portion, the buffer portion being located between the reaction cavity and the reagent storage cavity, the flow area of the buffer portion being larger than the flow area of the flow-through portion.

8. The reaction device according to claim 1, characterized in that, The reaction device further includes a sealing cover, and the reagent storage chambers include a first reagent storage chamber and a second reagent storage chamber; A part of the first reagent storage chamber extends beyond the main body and is provided with a sealing channel, the sealing channel communicating the reagent storage cavity and the external environment, and the sealing cover is sleeved on the part of the first reagent storage chamber extending beyond the main body and seals the sealing channel.

9. The reaction device according to claim 1, characterized in that, The reaction device further includes a sealing plate, the reagent storage chambers and the reaction chamber arch away from the main body to respectively form reagent storage cavities and reaction cavities, and the sealing plate is connected to a surface of the main body opposite to the reagent storage chambers and the reaction chamber for sealing the reagent storage cavities and the reaction cavities; and / or, The driving member includes a piston portion and a piston rod that are connected to each other. The piston portion is located in the reaction chamber, and the length of the piston rod is greater than the length of the reaction chamber.

10. A method for sequential reaction of reagents, characterized in that, Applied to the reaction device according to any one of claims 1 to 9, the method includes: A. Move the unlocking member to communicate the reagent storage chamber storing the reagent with the external environment; B. Drive the driving member in a direction away from the reaction chamber to control the reagent in the reagent storage chamber communicating with the external environment to flow into the reaction chamber; C. Repeat steps A and B so that the reagents in all the reagent storage chambers flow into the reaction chamber in sequence for reaction; D. Drive the driving member in a direction into the reaction chamber so that the mixed reagent returns to the reagent storage chamber.

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