Periodontitis H2S detection tooth socket based on MOF microporous adsorption effect and visual probe

Abstract

The invention relates to the technical field of oral medicine detection, in particular to a periodontitis H2S detection tooth socket based on an MOF micropore adsorption effect and a visual probe, which comprises a tooth socket and a detection layer coated on the inner surface of the tooth socket, h2S gas generated by periodontal pathogenic bacteria is efficiently adsorbed by utilizing the high specific surface area and microporous adsorption effect of the MOF material, the concentration of H2S can be preliminarily judged through naked eye observation, meanwhile, low-concentration H2S can be accurately detected in combination with a surface enhanced Raman scattering technology of AuBps, the detection sensitivity and accuracy are improved, and the existing research shows that the H2S detection method has the advantages that the H2S detection method is easy to implement, and the application prospect is wide. Periodontal pathogenic bacteria generate hydrogen sulfide (H2S), and the concentration of the hydrogen sulfide (H2S) is related to the severity of periodontitis and can be used as a detection index, so that through the detection tooth socket disclosed by the invention, rapid and high-sensitivity detection of periodontitis is realized, household or daily monitoring requirements are met, the oral health management level is improved, early discovery and intervention of periodontal diseases are facilitated, and the periodontal diseases are prevented from being damaged. And scientific and refined levels of oral health management are improved.

Description

Technical Field

[0001] The present invention relates to the technical field of oral medicine detection, and particularly to a periodontitis H2S detection dental appliance based on the microporous adsorption effect of MOF and a visual probe. Background Art

[0002] With the improvement of health awareness, the public's attention to oral health has increased significantly, especially the urgent need for early detection and treatment of common diseases such as periodontitis.

[0003] Periodontitis may not only cause oral problems such as gingival bleeding and tooth loosening, but also exacerbate systemic diseases such as diabetes and cardiovascular diseases, seriously endangering the health of patients. As a common chronic oral disease, early diagnosis is crucial for the treatment effect. However, the existing diagnosis of periodontitis mainly relies on clinical examinations, which are complex and require professional medical equipment, making it difficult to meet the needs of home or daily monitoring. In addition, due to the uneven distribution of medical resources, it is inconvenient for some patients to seek medical treatment.

[0004] Therefore, in view of the problems of requiring professional medical equipment, being difficult to meet the needs of home or daily monitoring, and the inconvenience of seeking medical treatment for some patients, a periodontitis H2S detection dental appliance based on the microporous adsorption effect of MOF and a visual probe can be designed. Summary of the Invention

[0005] In order to overcome the problems of requiring professional medical equipment, being difficult to meet the needs of home or daily monitoring, and the inconvenience of seeking medical treatment for some patients.

[0006] The technical solution of the present invention is: a periodontitis H2S detection dental appliance based on the microporous adsorption effect of MOF and a visual probe, including a dental appliance and a detection layer coated on the inner surface of the dental appliance.

[0007] Preferably, the dental appliance is made of TPE material.

[0008] Preferably, the detection layer is a mixed solution of N,N - diethyl - p - phenylenediamine, gold nanobipyramids, and γ - CD - MOF - Pluronics.

[0009] The present invention also provides a preparation method for a periodontitis H2S detection dental appliance based on the microporous adsorption effect of MOF and a visual probe, and the steps are as follows:

[0010] S1: Use TPE material to 3D print the dental appliance according to the model;

[0011] S2: Synthesize γ - CD - MOF using γ - cyclodextrin and potassium hydroxide, wash it with methanol and polyethylene glycol multiple times to form uniform nanoscale MOF crystals, and then add polyoxyethylene - polyoxypropylene - polyoxyethylene block copolymer to synthesize γ - CD - MOF - Pluronics;

[0012] S3: Mix N,N - diethyl - p - phenylenediamine with gold nanobipyramids, and then add them into the above - mentioned γ - CD - MOF - Pluronics solution to form a composite detection material;

[0013] S4: Coat the inner surface of the dental appliance with the composite detection material to form a detection layer.

[0014] Preferably, its usage method is as follows:

[0015] (1) The user wears the detection dental appliance for 2 hours. The detection layer on the inner surface of the dental appliance adsorbs periodontal pathogenic bacteria to produce H2S;

[0016] (2) The probe in the detection layer will react with H2S, resulting in an obvious color change, from bright yellow to pink - purple or blue - purple. The user can visually observe and preliminarily judge the risk of periodontitis;

[0017] (3) If a more accurate judgment or concentration is desired, use a Raman spectrometer to scan the detection sample, and perform precise quantitative detection of H2S through the Raman signal enhanced by AuBps;

[0018] (4) The user removes the dental appliance, takes a photo of its inner side and uploads it to the self - developed mini - program "Gingival Cloud Butler". This mini - program records and analyzes the data, creates a health dynamic change, and at the same time, the results will be sent to the corresponding attending doctor. After understanding all the data, the doctor will give customized health advice.

[0019] The beneficial effects of the present invention: Utilize the high specific surface area and microporous adsorption effect of the MOF material to efficiently adsorb the H2S gas produced by periodontal pathogenic bacteria. Select N,N - diethyl - p - phenylenediamine as the probe. After it covalently bonds with H2S to form a methylene blue - like complex, an obvious color change will occur, and the H2S concentration can be preliminarily judged by visual observation. At the same time, combined with the surface - enhanced Raman scattering technology of AuBps, low - concentration H2S can be accurately detected, improving the detection sensitivity and accuracy. The detection results can be recorded and analyzed through the supporting mini - program "Gingival Cloud Butler" to provide intuitive health feedback for users. Existing research shows that periodontal pathogenic bacteria produce hydrogen sulfide (H2S), and its concentration is related to the severity of periodontitis and can be used as a detection index. Therefore, through the detection dental appliance of the present invention, rapid and highly sensitive detection of periodontitis can be achieved, meeting the needs of home or daily monitoring, improving the level of oral health management, contributing to the early detection and intervention of periodontal diseases, and enhancing the scientific and refined level of oral health management. Description of the Drawings

[0020] Figure 1 Shown is the first three - dimensional structure schematic diagram of the periodontitis H2S detection dental appliance of the present invention based on the MOF microporous adsorption effect and visual probe;

[0021] Figure 2 The following is a schematic diagram of the periodontitis H2S detection dental appliance based on the MOF microporous adsorption effect and visualization probe of the present invention in different states. Among them, A is a schematic diagram of the 3D printed dental appliance finished product, B is the unused dental appliance, C is the dental appliance used by a user without periodontitis, and D is the dental appliance used by a user with local periodontitis.

[0022] Explanation of reference numerals: 1. Dental appliance; 2. Detection layer. Specific embodiments

[0023] The present invention will be further described below in conjunction with the drawings and embodiments.

[0024] As used herein, the term "gold nanobipyramid" is abbreviated as AuBps.

[0025] Please refer to Figure 1 - Figure 2 , the present invention provides an embodiment: a periodontitis H2S detection dental appliance based on the MOF microporous adsorption effect and visualization probe, including a dental appliance 1 and a detection layer 2 coated on the inner surface of the dental appliance 1.

[0026] Preferably, the dental appliance 1 is made of TPE material.

[0027] Preferably, the detection layer 2 is a mixed solution of N,N-diethyl-p-phenylenediamine, gold nanobipyramids, and γ-CD-MOF-Pluronics.

[0028] The present invention also provides a preparation method for a periodontitis H2S detection dental appliance based on the MOF microporous adsorption effect and visualization probe, and the steps are as follows:

[0029] S1: Use TPE material to 3D print the dental appliance 1 according to the model;

[0030] S2: Synthesize γ-CD-MOF using γ-cyclodextrin and potassium hydroxide, and wash it with methanol and polyethylene glycol multiple times to form uniform nanoscale MOF crystals, and then add polyoxyethylene-polyoxypropylene-polyoxyethylene block copolymer to synthesize γ-CD-MOF-Pluronics;

[0031] S3: Mix N,N-diethyl-p-phenylenediamine with gold nanobipyramids, and then add it to the above γ-CD-MOF-Pluronics solution to form a composite detection material;

[0032] S4: Coat the composite detection material on the inner surface of the dental appliance 1 to form the detection layer 2.

[0033] Preferably, its usage method is as follows:

[0034] (1) The user wears the detection dental appliance for 2 hours, and the detection layer 2 on the inner surface of the dental appliance 1 adsorbs periodontal pathogenic bacteria to produce H2S;

[0035] (2) The probe in the detection layer 2 will react with H2S, resulting in an obvious color change from bright yellow to pink-purple or blue-purple. The user can visually observe and preliminarily judge the periodontitis risk;

[0036] (3) If a more accurate judgment or concentration is desired, use a Raman spectrometer to scan the detection sample, and perform precise quantitative detection of H2S through the Raman signal enhanced by AuBps;

[0037] (4) The user removes the dental appliance 1, takes a photo of its inner side and uploads it to the self-developed mini-program "Gingival Cloud Butler". This mini-program records and analyzes the data, creates a health dynamic change, and at the same time the results will be sent to the corresponding attending doctor. After understanding all the data, the doctor will give customized health advice.

[0038] Example 1

[0039] (1) Use TPE material to 3D print the dental appliance 1 according to the model;

[0040] (2) Mix 648 mg of γ-CD and 224 mg of KOH in 20 mL of pure water, and pre-add 12 mL of MeOH to make a mother liquor. Then seal the mixture and place it in a glass container. Ultrasonically process the mixed solution for 5 minutes to obtain a clear solution. Then, quickly add 32 mL of methanol and 256 mg of polyethylene glycol to the reaction solution, heat the solution at 50 °C for 10 minutes. After 60 minutes, separate and collect the nanoscale MOF crystals, wash them twice with ethanol, and vacuum dry them overnight at 50 °C. Immerse the γ-CD-MOFs in a 5% (v / v) Pluronic ethyl acetate solution at room temperature for 33 hours, wash the material with fresh ethyl acetate, and then filter three times. Finally, dry it overnight in a vacuum oven at 50 °C;

[0041] (3) After mixing N,N-diethyl-p-phenylenediamine with AuBps, add the above solution, and then coat it on the inner surface of the dental appliance 1.

[0042] Experiments show that when the probe N,N-diethyl-p-phenylenediamine and NaHS are in a 1:3 ratio, the color development rate is the fastest and the reaction time is significantly shortened; it can still produce a visible color change under 10 -4 M NaHS, with relatively high sensitivity; the SERS combined with AuBps can achieve precise quantitative detection of H2S with a concentration as low as 10 -7 M.

[0043] Example 2

[0044] (1) The user wears the detection dental appliance prepared in Example 1 for 2 hours. The detection layer 2 on the inner surface of the dental appliance 1 adsorbs periodontal pathogenic bacteria to produce H2S;

[0045] (2) The probe in the detection layer 2 reacts with H2S, resulting in an obvious color change, from bright yellow to pink-purple or blue-purple. The user observes with the naked eye to preliminarily judge the periodontitis risk;

[0046] (3) If a more accurate judgment or concentration is desired, use a Raman spectrometer to scan the detection sample, and perform precise quantitative detection of H2S through the Raman signal enhanced by AuBps;

[0047] (4) The user removes the dental appliance 1, takes a photo of its inner side and uploads it to the independently developed small program "Gingival Cloud Butler". This small program records and analyzes the data, creates a dynamic health record, and at the same time the results will be sent to the corresponding attending doctor. After understanding all the data, the doctor will give customized health advice.

[0048] Through the above steps, by utilizing the high specific surface area and microporous adsorption effect of the MOF material, the H2S gas produced by periodontal pathogenic bacteria is efficiently adsorbed. N,N-diethyl-p-phenylenediamine is selected as the probe, and after it is covalently bonded with H2S to form a methylene blue-like complex, an obvious color change will occur, and the H2S concentration can be preliminarily judged by naked-eye observation. At the same time, combined with the surface-enhanced Raman scattering technology of AuBps, precise detection of low-concentration H2S can be carried out, improving the detection sensitivity and accuracy. Existing research shows that periodontal pathogenic bacteria produce hydrogen sulfide (H2S), and its concentration is related to the severity of periodontitis and can be used as a detection index. Therefore, through the detection dental appliance of the present invention, rapid and highly sensitive detection of periodontitis can be achieved, meeting the needs of home or daily monitoring, improving the level of oral health management, helping to detect and intervene in periodontal diseases at an early stage, and improving the scientific and refined level of oral health management to solve the problems that professional medical equipment is needed, it is difficult to meet the needs of home or daily monitoring, and it is inconvenient for some patients to seek medical treatment.

[0049] The embodiments of the present invention have been described in detail above in conjunction with the accompanying drawings. However, the present invention is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the gist of the present invention.

Claims

1. Periodontitis H2S detection brace based on MOF microporous adsorption effect and visualization probe, characterized by: The invention comprises a dental brace (1) and a detection layer (2) coated on the inner surface of the dental brace (1).

2. The periodontitis H2S detection dental brace based on MOF microporous adsorption effect and visualization probe according to claim 1, characterized in that: The dental cap (1) is made of TPE material.

3. The periodontitis H2S detection dental brace based on MOF microporous adsorption effect and visualization probe according to claim 1, characterized in that: The detection layer (2) is a mixed solution of N,N-diethyl-p-phenylenediamine, gold nanobipyramids and γ-CD-MOF-Pluronics.

4. The periodontitis H2S detection brace based on MOF microporous adsorption effect and visualization probe according to claim 1 is characterized in that: The preparation steps are as follows: S1: Using TPE material to 3D print the braces according to the model (1); S2: γ-CD-MOF was synthesized using γ-cyclodextrin and potassium hydroxide, washed with methanol and polyethylene glycol multiple times to form uniform nano-scale MOF crystals, and then polyoxyethylene-polyoxypropylene-polyoxyethylene block copolymer was added to synthesize γ-CD-MOF-Pluronics; S3: mixing N,N-diethyl-p-phenylenediamine with gold nanobipyramids, and then adding them to the above-mentioned γ-CD-MOF-Pluronics solution to form a composite detection material; S4: Covering the composite detection material on the inner surface of the dental brace (1) to form a detection layer (2).

5. The periodontitis H2S detection dental brace based on MOF microporous adsorption effect and visualization probe according to claim 4, characterized in that: Here’s how to use it: (1) The user wears the detection brace for 2 hours, and the detection layer (2) on the inner surface of the brace (1) absorbs periodontal pathogens to produce H2S; (2) The probe in the detection layer (2) reacts with H2S, producing a significant color change from bright yellow to pink or purple. The user can make a preliminary judgment on the risk of periodontitis by visual observation; (3) If you want to get a more accurate judgment or concentration, use a Raman spectrometer to scan the test sample and accurately and quantitatively detect H2S through the Raman signal enhanced by AuBps; (4) The user takes off the braces (1), takes a photo of the inner side and uploads it to the self-developed mini program "Gum Cloud Manager". The mini program records and analyzes the data to produce dynamic health changes. The results will be delivered to the corresponding attending physician. After understanding all the data, the doctor will give customized health suggestions.

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