Personal care composition

Abstract

Disclosed is a personal care composition comprising a carboxymethyl cysteine compound and coffee seed oil, wherein the weight ratio of carboxymethyl cysteine compound to coffee seed oil is from 1: 200 to 12: 1.

Description

Technical Field

[0001] The present invention relates to a personal care composition comprising a carboxymethylcysteine compound and coffee seed oil. Surprisingly, such a composition is capable of providing a synergistic antioxidant effect. Background Art

[0002] Human skin can provide physicochemical protection against environmental damage through its barrier function, mechanical strength, and water impermeability. It is often exposed to harmful environments and extreme conditions such as ultraviolet radiation, blue light, pollution, etc. Such harmful environmental exposures may lead to the overproduction of reactive oxygen species (ROS), resulting in oxidative stress conditions. Oxidative stress can affect our skin in many ways, including signs of premature aging such as wrinkles, fine lines, and uneven skin tone.

[0003] Thioredoxin reductase 1 (TXNRD1) is a redox enzyme encoded by the TXNRD1 gene in humans and is a member of the antioxidant thioredoxin system. TXNRD1 plays an important role in oxidative stress control. It reduces and activates thioredoxin, which is a redox enzyme containing a dithiol-disulfide active site. Thioredoxin binds to ROS before they can damage cells and thus protects cells from oxidative stress.

[0004] The inventors have recognized the need to develop personal care compositions that can improve the gene expression of TXNRD1. Therefore, the inventors have developed a personal care composition comprising a carboxymethylcysteine compound and coffee seed oil. Surprisingly, it has been found that by combining the carboxymethylcysteine compound with coffee seed oil, and when the weight ratio of the carboxymethylcysteine compound to coffee seed oil is from 1:200 to 12:1, the expression of the TXNRD1 gene is synergistically increased. Summary of the Invention

[0005] In a first aspect, the present invention relates to a personal care composition comprising a carboxymethylcysteine compound and coffee seed oil, wherein the weight ratio of the carboxymethylcysteine compound to coffee seed oil is from 1:200 to 12:1.

[0006] In a second aspect, the present invention relates to a method of providing antioxidant and / or anti-aging benefits, reducing oxidative stress, and / or upregulating the expression of the thioredoxin reductase 1 gene, the method comprising the step of topically applying the composition of the present invention to the skin.

[0007] In a third aspect, the present invention relates to the use of the composition of the present invention for providing antioxidant and / or anti-aging benefits; reducing oxidative stress; and / or upregulating the expression of the thioredoxin reductase 1 gene.

[0008] All other aspects of the present invention will become more readily apparent by considering the following detailed description and examples. Detailed Description of the Invention

[0010] Unless in the examples or otherwise explicitly indicated, all numbers in this specification indicating amounts of materials or reaction conditions, physical properties of materials, and / or uses may optionally be understood to be modified by the word "about".

[0011] Unless otherwise specified, all amounts are by weight of the composition.

[0012] It should be noted that when specifying any range of values, any particular upper value can be associated with any particular lower value.

[0013] For the avoidance of doubt, the word "comprising" is intended to mean "including" but not necessarily "consisting of" or "composed of". In other words, the listed steps or options need not be exhaustive.

[0014] The disclosure of the present invention as found herein is considered to cover all embodiments as found in the claims, as they cross-reference each other, regardless of the fact that the claims may be found to be without cross-reference or redundant.

[0015] In the case of disclosing features regarding a particular aspect of the present invention (e.g., the composition of the present invention), with the necessary modifications, such disclosure is also considered applicable to any other aspect of the present invention (e.g., the method of the present invention).

[0016] The carboxymethylcysteine compound refers to a compound selected from carboxymethylcysteine, a salt of carboxymethylcysteine, an ester of carboxymethylcysteine, an amide of carboxymethylcysteine, or a mixture thereof. Preferably, the carboxymethylcysteine compound includes carboxymethylcysteine, an ester of carboxymethylcysteine, and / or a salt of carboxymethylcysteine. More preferably, the carboxymethylcysteine compound includes carboxymethylcysteine and / or a salt of carboxymethylcysteine. Even more preferably, the carboxymethylcysteine compound contains a salt of carboxymethylcysteine. Still even more preferably, the carboxymethylcysteine compound contains lysine carboxymethylcysteine salt, and most preferably, the carboxymethylcysteine compound is lysine carboxymethylcysteine salt.

[0017] Preferably, the carboxymethylcysteine compound is present in an amount of at least 0.00001%, more preferably at least 0.0001%, even more preferably at least 0.001%, still even more preferably at least 0.01%, and most preferably at least 0.1% by weight of the composition. Preferably, the carboxymethylcysteine compound is present in an amount of not more than 10%, more preferably not more than 5%, even more preferably not more than 3%, still even more preferably not more than 1%, and most preferably not more than 0.5% by weight of the composition.

[0018] Preferably, the lysine carboxymethylcysteine salt is present in an amount of not more than 10%, more preferably not more than 5%, even more preferably not more than 3%, still even more preferably not more than 1%, and most preferably not more than 0.5% by weight of the composition. Preferably, the lysine carboxymethylcysteine salt is present in an amount of at least 0.00001%, more preferably at least 0.0001%, even more preferably at least 0.001%, still even more preferably at least 0.01%, and most preferably at least 0.1% by weight of the composition.

[0019] Coffee seed oil is an oil extracted from the beans of certain coffee species. It can be extracted from unroasted coffee beans or roasted coffee beans. Coffee seed oil is rich in saturated fatty acids, unsaturated fatty acids and tocopherols. Due to these properties, coffee seed oil is sometimes used in skin care products. The composition of coffee seed oil varies according to the manufacturing method and coffee species. For example, unroasted coffee seed oil may contain higher levels of fatty acids, vitamins and caffeine.

[0020] Koffee’UP from Givaudan TM is an oil from roasted Arabica coffee seeds. It is manufactured by drying spent coffee grounds and then by supercritical CO2 extraction. Koffee’UP TM is alleged to protect the skin and help the skin stay hydrated.

[0021] The composition of the present invention contains coffee seed oil. Preferably, the coffee seed oil is extracted from roasted coffee seeds, more preferably from roasted coffee seeds selected from: Arabica coffee, Coffea canephora Pierre and Coffea liberica Bull ex Hiern, and most preferably from roasted coffee seeds from Arabica coffee. Preferably, the coffee seed oil is obtained by supercritical fluid extraction, more preferably by supercritical CO2 extraction.

[0022] Preferably, the coffee seed oil is used in the composition in an amount of 0.00001% to 5% by weight of the composition, more preferably in an amount of 0.0001% to 2% by weight of the composition, even more preferably in an amount of 0.02% to 0.2% by weight of the composition.

[0023] The weight ratio of the carboxymethylcysteine compound to the coffee seed oil is from 1:200 to 12:1, more preferably from 1:50 to 12:1, even more preferably from 1:12 to 3:1, still even more preferably from 1:6 to 1.5:1, and most preferably from 4:1 to 1:1.

[0024] For clarity, the amount of the extract in the present invention refers to the amount of the components extracted from the plant and does not include the weight of the extraction solvent.

[0025] Preferably, the composition contains a vitamin B3 compound (including derivatives of vitamin B3). The vitamin B3 compound includes nicotinic acid, niacin, nicotinamide or a mixture thereof. The most preferred vitamin B3 compound is nicotinamide. The amount of the vitamin B3 compound can be 0.1 to 10% by weight, preferably 0.5 to 5% by weight of the composition.

[0026] Preferably, the composition contains a glutamic acid source selected from the group consisting of glutamine, glutamine esters, glutamic acid, pyroglutamic acid, salts and mixtures thereof. More preferably, the composition contains pyroglutamic acid and / or a salt of pyroglutamic acid. Even more preferably, the composition contains the sodium salt of pyroglutamic acid. Preferably, the amount of the glutamic acid source present is 0.0001 - 10% by weight of the composition, more preferably 0.001 - 6% by weight of the composition, even more preferably 0.01 - 3% by weight.

[0027] The composition may optionally contain a whitening pigment. The whitening pigment is usually particles of a high refractive index material. For example, the refractive index of the whitening pigment can be greater than 1.3, more preferably greater than 1.8, and most preferably 2.0 - 2.7. Examples of such whitening pigments are those containing bismuth oxychloride, boron nitride, barium sulfate, mica, silica, titanium dioxide, zirconium oxide, aluminum oxide, zinc oxide or combinations thereof. More preferred whitening pigments are particles containing titanium dioxide, zinc oxide, zirconium oxide, mica, iron oxide or combinations thereof. Even more preferred whitening pigments are particles containing zinc oxide, zirconium oxide, titanium dioxide or combinations thereof because these materials have particularly high refractive indices. Still even more preferably, the whitening pigment is selected from titanium dioxide, zinc oxide or a mixture thereof, and the most preferred whitening pigment is titanium dioxide. The average diameter of the whitening pigment is usually 15 nm - 1 μm, more preferably 35 nm - 800 nm, even more preferably 50 nm - 500 nm, still even more preferably 100 - 300 nm. The amount of the whitening pigment can be 0.1 - 15% by weight of the composition, preferably 0.5 - 5% by weight.

[0028] Preferably, the composition comprises a polyol. The polyol may be selected from glycerol, propylene glycol, dipropylene glycol, polypropylene glycol, polyethylene glycol, sorbitol, hydroxypropyl sorbitol, hexylene glycol, 1,3 - butanediol, isopentylene glycol, ethoxylated glycerol, propoxylated glycerol, or mixtures thereof. The most preferred polyol is glycerol, also known as glycerin. The amount of the polyol may be 0.1 - 20% by weight of the composition, preferably 0.5 - 15% by weight, more preferably 2 - 10% by weight.

[0029] Preferably, the composition comprises an emollient material. Suitable emollient materials include silicones, hydrocarbons, triglycerides, or mixtures thereof. These silicones can be organic, silicone - containing, or fluorine - containing, volatile or non - volatile, polar or non - polar. Hydrocarbons may include mineral oil, petrolatum, and polyalpha - olefins. Examples of preferred volatile hydrocarbons include polydecane, such as isododecane and isodecane (e.g., Permethyl - 99A available from Presperse Inc.), and C7 - C8 to C12 - C15 isoparaffins (e.g., the Isopar series available from Exxon Chemicals). Exemplary triglycerides are, but not limited to, sunflower oil, cottonseed oil, canola oil, soybean oil, castor oil, borage oil, olive oil, shea butter, jojoba oil, and mixtures thereof. Glycerol monoesters and glycerol diesters may also be used. Particularly preferred are glycerol monostearate and glycerol distearate.

[0030] Preferably, the composition comprises a humectant. Particularly preferred humectants include petrolatum, aquaporin - modulating actives, oat kernel flour, substituted ureas such as hydroxyethyl urea, hyaluronic acid and / or its precursor N - acetylglucosamine, hyaluronic acid and / or its precursor N - acetylglucosamine, or mixtures thereof.

[0031] Some compositions may comprise a thickening agent. These may be selected from celluloses, natural gums, and acrylic polymers, but are not limited to these types of thickening agents. Cellulose - based ones include sodium carboxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl methyl cellulose, and combinations thereof. Suitable gums include xanthan gum, pectin, karaya gum, agar, alginate gums, and combinations thereof. Acrylic thickening agents are homopolymers and copolymers of acrylic acid and methacrylic acid, including carbomers, such as Carbopol 1382, Carbopol 982, Ultrez, Aqua SF - 1, and Aqua SF - 2 available from Lubrizol Corporation. The amount of the thickening agent may be 0.01 - 3% by weight of the active polymer (excluding solvents or water) in the composition.

[0032] In addition, the composition of the present invention may further comprise 0.5 to 10% by weight of a chelating agent such as tetrasodium ethylenediaminetetraacetate (EDTA), EHDP or a mixture thereof; a light-shielding agent and a pearlescent agent such as ethylene glycol distearate, titanium dioxide or Lytron 621 (styrene / acrylic ester copolymer); all of which can be used to enhance the appearance or properties of the product.

[0033] The composition may contain water in an amount of 10 to 96% by weight, more preferably 25 to 92% by weight, even more preferably 42 to 88% by weight, and most preferably 55 to 82% by weight, based on the weight of the composition.

[0034] Preferably, when measured at 20 °C at a relatively high shear rate of about 20 s -1 -1, the viscosity of the composition is at least 10 mPa·s, more preferably 30 to 10,000 mPa·s, even more preferably 50 to 5,000 mPa·s, and most preferably 100 to 2,000 mPa·s.

[0035] Preferably, the composition is an emulsion, more preferably an oil-in-water emulsion. Preferably, the composition is a fluid liquid at 25 °C and atmospheric pressure.

[0036] Preferably, the personal care composition is a skin care composition. A skin care composition refers to a composition suitable for topical application to human skin, including leave-on and rinse-off products, but preferably leave-on compositions. The term "leave-on" as used herein with respect to the compositions of the present invention refers to a composition applied to the skin or rubbed on the skin and left on the skin. The term "rinse-off" as used herein with respect to the compositions of the present invention refers to a skin cleanser applied to the skin or rubbed on the skin and rinsed off substantially immediately after application. As used herein, the term "skin" includes the skin on the face, neck, chest, abdomen, back, arms, underarms, hands and legs. Preferably, "skin" is meant to include the skin on the face and underarms, and more preferably, skin is meant to include the skin on the face except for the lips and eyelids. The composition is particularly preferably a moisturizer rather than a cosmetic product.

[0037] Preferably, the composition is a topical composition. Preferably, the composition can be in the form of a cream, lotion, ointment, solution, suspension, emulsion, paste, gel, powder, foundation, liquid foundation, wax-based foundation or spray. More preferably, the composition can be formulated in the form of a cream, lotion, ointment, emulsion, gel or spray.

[0038] Preferably, the use is non-therapeutic. Preferably, the method is non-therapeutic. The term non-therapeutic generally refers to being for cosmetic purposes rather than for curative or therapeutic purposes.

[0039] The following examples are provided to facilitate understanding of the present invention. These examples are not intended to limit the scope of the claims. Detailed implementation mode

[0040] Example

[0041] Material

[0042]

[0043] *The *Koffee’UP used in the example TM (Arabica coffee seed oil) was obtained from Givaudan France SAS. Used to prepare Koffee’UP TM The Arabica coffee seeds are not obtained from India.

[0044] Example 1

[0045] This example demonstrates the synergistic upregulation of gene expression by combining carboxymethylcysteine compounds and coffee seed oil.

[0046] Normal human epidermal keratinocytes (NHEK, Archgene Biotechnology, Shanghai, China, batch number Lot: NHS-2021-006) were incubated in medium with or without the active ingredient for 24 hours. After incubation, total RNA of each NHEK was extracted using the Qiagen Mini RNeasy kit (Qiagen, Cat.74106) according to the manufacturer's protocol.

[0047] The extracted RNA was quantified using a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, US), and reverse transcription was performed using ABScript III RT Mast Mix (Cat. BK20428, ABclonal) according to the manufacturer's protocol to generate template cDNA. The gene encoding the TXNRD1 (thioredoxin reductase 1) gene was amplified using RT-PCR reagents (Universal SYBR Green Fast qPCR Mix kit (Cat. RK21203, ABclonal)) on an ABI Vi7 Real-Time PCR system (Applied Biosystems, Thermo fisher scientific, Carlsbad, CA, USA). Tyrosine 3-monooxygenase / tryptophan 5-monooxygenase activation protein Zeta (YWHAZ) was selected as the housekeeping gene, and all data on the relative expression of the target gene were normalized to YWHAZ. The fold change in expression was calculated relative to a blank control (medium without active substance). All experiments were performed at least three times, and Table 1 shows the fold change in TXNRD1 gene expression.

[0048] Table 1

[0049]

[0050] #: Based on the level of active substance in the medium.

[0051] a: Significantly better than (p < 0.05) any of the individual active substances of the same level.

[0052] As shown in Table 1, it was surprisingly found that the fold change when lysine carboxymethyl cysteine and coffee seed oil were combined at a specific ratio was significantly higher than the product of the fold changes of lysine carboxymethyl cysteine alone and coffee seed oil alone. By combining lysine carboxymethyl cysteine and coffee seed oil within the scope of the present invention, the expression of TXNRD1 was synergistically increased. In contrast, when lysine carboxymethyl cysteine and coffee seed oil were combined outside the scope of the present invention, the expression of the TXNRD1 gene was not increased.

Claims

1. A personal care composition comprising a carboxymethylcysteine compound and coffee seed oil, wherein the weight ratio of the carboxymethylcysteine compound to the coffee seed oil is from 1:200 to 12:

1.

2. The composition according to claim 1, wherein the carboxymethylcysteine compound comprises carboxymethylcysteine, an ester of carboxymethylcysteine, and / or a salt of carboxymethylcysteine.

3. The composition according to claim 2, wherein the carboxymethylcysteine compound comprises a salt of carboxymethylcysteine, preferably the carboxymethylcysteine compound comprises lysine carboxymethylcysteine salt.

4. The composition according to any one of the preceding claims, wherein the carboxymethylcysteine compound is present in an amount of at least 0.00001% by weight and not more than 10% by weight based on the weight of the composition, preferably the carboxymethylcysteine compound is present in an amount of at least 0.01% by weight and not more than 3% by weight based on the weight of the composition.

5. The composition according to any one of the preceding claims, wherein the coffee seed oil is extracted from roasted coffee seeds, more preferably from roasted coffee seeds selected from: Arabica coffee, Coffea canephora Pierre ex A. Froehner, and Coffea liberica Bull ex Hiern, most preferably from roasted coffee seeds of Arabica coffee.

6. The composition according to any one of the preceding claims, wherein the amount of the coffee seed oil is from 0.00001% by weight to 5% by weight of the composition, preferably from 0.02% by weight to 0.2% by weight of the composition.

7. The composition according to any one of the preceding claims, wherein the weight ratio of the carboxymethylcysteine compound to the coffee seed oil is from 1:50 to 12:1, preferably 1:12 to 3:

1.

8. The composition according to any one of the preceding claims, wherein the composition is an emulsion, preferably an oil-in-water emulsion.

9. The composition according to any one of the preceding claims, wherein the composition is a fluid liquid at 25°C and atmospheric pressure.

10. The composition according to any one of the preceding claims, wherein the composition comprises water in an amount of from 10% by weight to 96% by weight based on the weight of the composition, preferably from 42% by weight to 88% by weight based on the weight of the composition.

11. A method of providing antioxidant and / or anti-aging benefits, reducing oxidative stress and / or upregulating the expression of the thioredoxin reductase 1 gene, the method comprising the step of topically applying the composition according to any one of the preceding claims to the skin.

12. Use of the composition according to any one of the preceding claims 1-10 for providing antioxidant and / or anti-aging benefits, reducing oxidative stress and / or upregulating the expression of the thioredoxin reductase 1 gene.

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