Cosmetic base oil capable of efficiently enriching whitening active ingredients and preparation method of cosmetic base oil

Abstract

The embodiment of the invention discloses cosmetic base oil capable of efficiently enriching whitening active ingredients and a preparation method of the cosmetic base oil, relates to the technical field of cosmetics, and aims at solving the technical problem that the preparation efficiency of existing cosmetic base oil is relatively low. According to the cosmetic base oil capable of efficiently enriching the whitening active ingredients, the cosmetic base oil comprises plant effective ingredients and a surface active agent, and the use amount of the surface active agent is 1%-5% of the mass of the plant effective ingredients; the plant effective component comprises a plant raw material and a plant oil material liquid in a weight ratio of (1: 4)-(1: 10).

Description

Technical Field

[0001] The present application relates to the technical field of cosmetics, and particularly relates to a cosmetic base oil for efficiently enriching whitening active ingredients and a preparation method thereof. Background Art

[0002] The skin, which consists of the epidermis and the dermis, is the outermost layer that isolates the external environment from the human body. In the epidermis, melanocytes affect human skin color by producing melanin. Melanin can reduce oxidative stress and ultraviolet damage, so it has a protective effect on the skin. However, excessive melanin production can lead to pigmentation diseases, such as melasma, freckles, melanosis, etc. Skin whitening refers to using natural or synthetic substances to lighten the skin color or provide a uniform skin color by reducing the melanin content in the skin. There are mainly two ways to whiten the skin. The first way is to promote the absorption, metabolism or excretion of melanin, and there are methods such as inhibiting the proliferation of melanocytes, reducing melanin intermediates and promoting the exfoliation of the stratum corneum. The second way is to inhibit the activity of enzymes related to melanin synthesis. In the process of melanin formation, three enzymes mainly play a role, namely tyrosinase, TRP-1, and TRP-2. Among them, tyrosinase plays a key role in regulating melanin synthesis. In recent years, tyrosinase has become an important target for developing whitening skin care products. Common whitening agents include kojic acid, hydroquinone, and hydroquinone, etc. However, these chemically synthesized whitening agents may cause some side effects such as dermatitis, erythema, and swelling, and cause permanent damage to skin cells. Therefore, with the gradual increase of people's environmental awareness and safety concerns about synthetic chemical components, natural plant active ingredients have gradually been favored in cosmetic formulations, such as resveratrol, hydroxytyrosol, and arbutin, etc. How to efficiently obtain these active ingredients has become a research hotspot for developing whitening cosmetics at home and abroad.

[0003] Skin lipids are mainly composed of sebum secreted by sebaceous glands and lipids produced by stratum corneum cells. Since vegetable oils are similar in composition to the lipids that make up the skin structure, they can be used as important basic raw materials for cosmetics. Lipids are mainly divided into vegetable oils, animal oils, mineral oils, and synthetic oils. Vegetable oils are often used in cosmetics due to their natural source and mild properties. The application of vegetable oils in cosmetics has a history of thousands of years. The ancients often used vegetable oils to nourish their hair and made creams with olive oil, beeswax, etc. to apply on the skin to achieve the effect of nourishing the skin with oil. Oil-based skin care products are easier to penetrate into the skin than water-based skin care products, supplement the fatty acids needed by the skin, and improve the skin's moisturizing ability. The rich unsaturated fatty acids, tocopherols, sterols, polyphenols and other trace components contained in vegetable oils make them have the effect of nourishing the skin. In cosmetics, vegetable oils are usually used as base oils and can be directly applied to the face. The most common use is to dilute essential oils. There have been relevant research reports that many vegetable oils such as argan oil, rosehip oil, cactus seed oil, etc. have tyrosinase inhibitory effects, indicating the potential use of vegetable oils as functional raw materials in personal care and cosmetic preparations with whitening effects.

[0004] Among vegetable oils, in addition to unsaturated fatty acids such as oleic acid and linoleic acid having an inhibitory effect on melanin production, trace components, especially tocopherols, play an important antioxidant role. Vegetable oils usually have good solubility for fat-soluble components, so the content of water-soluble polyphenolic whitening active components is limited. Therefore, if such polyphenolic components can be enriched in vegetable oils without affecting their own quality, it is an effective method to solve the current problem of making safe and green minimalist cosmetic formulations. In addition to special vegetable oils (such as argan oil, sweet almond oil, etc.), traditional cosmetic base oils can also be obtained by plant maceration oil or solvent extraction methods. These methods have many operation steps, long time consumption, low extraction efficiency, and the solubility of target active components in vegetable oils is limited. The oxidation stability of vegetable oils is not high, resulting in limitations in their practical applications in daily chemical formulations. Summary of the Invention

[0005] The main purpose of this application is to provide a cosmetic base oil for efficiently enriching whitening active components and its preparation method, aiming to solve the technical problem of the low preparation efficiency of existing cosmetic base oils.

[0006] To achieve the above purpose, the technical solutions adopted in the embodiments of this application are as follows:

[0007] In the first aspect, the embodiments of this application provide a cosmetic base oil for efficiently enriching whitening active components. The cosmetic base oil includes plant active ingredients and a surfactant, and the dosage of the surfactant is 1% - 5% of the mass of the plant active ingredients; the plant active ingredients include a plant raw material and a vegetable oil liquid in a weight ratio of 1:4 - 1:10.

[0008] In some alternative embodiments of the present application, the vegetable oil refers to virgin oil extracted from fruits, seeds or germ of plants or its refined oil.

[0009] In some alternative embodiments of the present application, the surfactant refers to a substance with fixed hydrophilic and lipophilic groups that can be oriented on the surface of the solution and can significantly reduce the surface tension.

[0010] In some alternative embodiments of the present application, the surfactant is soy lecithin or glycerol monooleate.

[0011] In some alternative embodiments of the present application, the plant raw material refers to at least one of glabrous licorice and its processing by-products, and olive processing by-products; the olive processing by-products include olive leaves and / or olive pomace.

[0012] In a second aspect, embodiments of the present application provide: A method for preparing a cosmetic base oil for highly enriching whitening active ingredients as described above, comprising the following steps:

[0013] Mix the vegetable oil and the surfactant, heat and stir until the surfactant is completely dissolved, then add the plant raw material powder, and perform cell wall breaking extraction using a physical field strengthening technology, and centrifuge to collect the supernatant to obtain a cosmetic base oil for highly enriching whitening active ingredients.

[0014] In some alternative embodiments of the present application, the plant raw material powder refers to the plant raw material powder obtained by drying fresh plant leaves in an oven at 40°C, and then crushing the dried leaves through a pulverizer and passing through a 60-mesh standard sieve.

[0015] In some alternative embodiments of the present application, the stirring temperature for heating and stirring is 30-60°C, the stirring speed is 400-1000 r / min, and the stirring time is 0.5-12 h.

[0016] In some alternative embodiments of the present application, the addition amount of the surfactant is 1%-5%, and the ratio of the plant raw material to the vegetable oil is 1:4-1:10.

[0017] In some alternative embodiments of the present application, the physical field strengthening technology includes ultrasonic technology or microwave technology. When the physical field strengthening technology is ultrasonic technology, the conditions for cell wall breaking extraction are ultrasonic power of 300-500 W and treatment time of 20-55 min.

[0018] Compared with the prior art, the beneficial effects of the present application are:

[0019] This application proposes and elaborates in detail an innovative technology for preparing cosmetic base oil. The remarkable feature of this technology is that it does not use any organic solvents at all. Through a carefully designed process flow, this method has successfully extracted and enriched polyphenolic whitening active ingredients from a variety of natural plant sources, thus ensuring that the active ingredients contained in the cosmetic base oil are completely derived from natural plant extracts. During the preparation process, this application particularly focuses on the proportional relationship between the plant raw materials of the cosmetic base oil and the vegetable oil liquor. After repeated experiments and optimizations, the optimal weight ratio has finally been determined, that is, the weight ratio of the plant raw materials to the vegetable oil liquor is 1:4 to 1:10. In addition, in order to further improve the extraction performance of the cosmetic base oil, this application adds an appropriate amount of surfactant to promote the formation of associated colloids in the base oil, and the addition amount is controlled between 1% and 5%. The extraction efficiency is enhanced through ultrasonic cavitation effect or microwave heating. Another highlight of this application is the full utilization of the by-products generated during the processing of natural plant raw materials, such as olive leaves and olive pomace. These by-products are reused in this application, thus significantly improving the comprehensive utilization rate of raw material resources and environmental protection benefits. Finally, the cosmetic base oil prepared through this application not only contains richer whitening active ingredients, but also performs excellently in maintaining product stability, especially in terms of oxidation stability. Compared with the base oil prepared by traditional methods, the product prepared by this application exhibits more excellent performance. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a comparison chart of the total polyphenol content between the new cosmetic base oil involved in the embodiment of this application and other vegetable oils;

[0021] Figure 2 It is the total ion current chromatogram of liquid chromatography-mass spectrometry of polyphenolic whitening active ingredients obtained from the new cosmetic base oil involved in the embodiment of this application and extracted by the traditional solvent method; in the figure, A - the new cosmetic base oil obtained by the green preparation method, B - the extraction solution obtained by the traditional organic solvent extraction method;

[0022] Figure 3 It is an impact diagram of the new cosmetic base oil involved in the embodiment of this application and other cosmetic base oils on the skin irritation of New Zealand rabbits; in the figure, a - low dose (0.1 g), b - high dose (0.5 g), A - camellia seed oil, B - new cosmetic base oil, C - olive oil. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0023] It should be understood that the specific embodiments described herein are only used to explain this application and are not used to limit this application.

[0024] The skin consists of the epidermis and the dermis and serves as a barrier between the human body and the external environment. Melanocytes in the epidermis produce melanin, which protects the skin, but excessive amounts can lead to pigmentation disorders. Skin whitening aims to reduce the melanin concentration and even out skin tone. Whitening methods include promoting the absorption, metabolism, or excretion of melanin, as well as inhibiting the activity of synthetic enzymes. Tyrosinase is the key synthetic enzyme and is the focus of the development of whitening products. Chemical whitening agents may cause side effects, so natural plant components such as resveratrol, hydroxytyrosol, and arbutin are more popular.

[0025] Skin lipids are secreted by sebaceous glands and produced by stratum corneum cells. Vegetable oils, due to their similarity to skin lipids, are commonly used in cosmetics. Vegetable oils have a long history, can nourish the skin, and improve its moisturizing ability. They contain unsaturated fatty acids, tocopherols, etc., which have nourishing and antioxidant effects. As base oils, vegetable oils can dilute essential oils, and some vegetable oils themselves have whitening effects.

[0026] Unsaturated fatty acids and trace components such as tocopherols in vegetable oils have an inhibitory effect on melanogenesis and have antioxidant effects. The preparation process of traditional cosmetic base oils is complex, inefficient, and has poor stability.

[0027] Based on the above technical problems, this application will use natural vegetable oils as solvents to directly extract polyphenolic whitening active ingredients from plant materials, and based on the polarity phase paradox, by adding a certain amount of surfactant to the vegetable oil to regulate the self-assembly generation of nano-scale associated colloids, so as to enrich more polyphenolic whitening active ingredients in the vegetable oil, and greatly improve the oxidative stability of the oil. This innovative method not only effectively solves the problems of solvent residue safety and oil oxidative stability, but also ultrasonic or microwave extraction technology can assist in regulating the generation of associated colloids, greatly improving the extraction efficiency of polyphenolic whitening active ingredients.

[0028] Specifically, the cosmetic base oil described in this application includes plant active ingredients and surfactants, and the dosage of the surfactant is 1% - 5% of the mass of the plant active ingredients; the plant active ingredients include a plant raw material and a vegetable oil liquid with a weight ratio of 1:4 - 1:10.

[0029] It should be noted that in the technical solution of this application, the vegetable oil specifically refers to virgin oil extracted from the fruits, seeds or germ of plants by physical or chemical methods, or oil products further refined to remove impurities and impure substances. These oil products usually retain the natural characteristics of the plant raw materials and have a wide range of applications in the fields of food industry, cosmetics manufacturing and biofuel production. Surfactants, as another key component of this application, refer to compounds with distinct hydrophilic and lipophilic groups, which can form an oriented arrangement at the surface or interface of the solution, thereby significantly reducing the surface tension and enhancing the wetting, emulsifying and dispersing abilities of the liquid. This application particularly focuses on two surfactants, soy lecithin and glycerol monooleate, which are highly favored in industrial applications due to their unique physicochemical properties. As for the plant raw materials, this application focuses on at least one of glabrous licorice and its processing by-products, and olive processing by-products; the olive processing by-products include olive leaves and / or olive pomace. These raw materials not only have a long application history in traditional medicine and food fields, but also show their potential health benefits and industrial value in modern scientific research.

[0030] This application further discloses a method for preparing a cosmetic base oil for highly enriching whitening active ingredients, and the steps are as follows:

[0031] First, mix the vegetable oil and the surfactant, and heat and stir until the surfactant is completely dissolved. Subsequently, add the plant raw material powder thereto, and perform wall-breaking extraction on the mixture by using a physical field strengthening technology (such as ultrasonic wave or microwave). Collect the supernatant through centrifugation operation to obtain a cosmetic base oil rich in whitening active ingredients.

[0032] The preparation method of the plant raw material powder is: place the fresh plant leaf material in an oven, perform drying treatment at 40°C, then crush the dried leaves by a crusher, and screen through a 60-mesh standard sieve to finally obtain the required plant raw material powder.

[0033] During the heating and stirring process, the stirring temperature should be controlled within the range of 30 - 60°C, the stirring speed should be maintained at 400 - 1000 r / min, and the stirring time should be maintained between 0.5 - 12 hours.

[0034] The addition amount of the surfactant should be 1% - 5% of the total amount after mixing the vegetable oil and the plant raw material, and the ratio of the plant raw material to the vegetable oil should be 1:4 to 1:10.

[0035] When the physical field strengthening technology is ultrasonic technology, the conditions for wall-breaking extraction are ultrasonic power of 300 - 500 W and treatment time of 20 - 55 min.

[0036] For the convenience of those skilled in the art to understand the technical solution of this application, the following further elaborates on the technical solution of this application in combination with specific embodiments:

[0037] Example 1

[0038] Step 1: Take 25 g of camellia seed oil, add 1.5 g of soy lecithin, and stir at 40°C and 500 r / min until completely dissolved;

[0039] Step 2: Add 5 g of dried olive leaves to Step 1 and stir evenly;

[0040] Step 3: Place the material liquid in an ultrasonic reactor for extraction. Set the ultrasonic intensity to 450 W, the ultrasonic time to 35 min, set the alarm temperature to 70°C, and set a water bath during the ultrasonic process to ensure that the temperature does not exceed the alarm temperature. After the extraction is completed, centrifuge at 10000 rpm for 10 min, and take the supernatant, which is the cosmetic base oil.

[0041] Example 2

[0042] Step 1: Take 25 g of camellia seed oil, add 0.25 g of soy lecithin, and stir at 40°C and 500 r / min until completely dissolved;

[0043] Step 2: Add 1.7 g of dried olive leaves to Step 1 and stir evenly;

[0044] Step 3: Place the material liquid in an ultrasonic reactor for ultrasonic treatment. Set the ultrasonic intensity to 450 W, the ultrasonic time to 10 min, set the alarm temperature to 70°C, and set a water bath during the ultrasonic process to ensure that the temperature does not exceed the alarm temperature. After the extraction is completed, centrifuge at 10000 rpm for 10 min, and take the supernatant, which is the cosmetic base oil.

[0045] Example 3

[0046] Step 1: Take 25 g of camellia seed oil, add 2 g of glycerol monooleate, and stir at 40°C and 500 r / min until completely dissolved;

[0047] Step 2: Add 2.5 g of dried olive leaves to Step 1 and stir evenly;

[0048] Step 3: Place the material liquid in an ultrasonic reactor for ultrasonic treatment. Set the ultrasonic intensity to 300 W, the ultrasonic time to 35 min, set the alarm temperature to 70°C, and set a water bath during the ultrasonic process to ensure that the temperature does not exceed the alarm temperature. After the extraction is completed, centrifuge at 10000 rpm for 10 min, and take the supernatant, which is the cosmetic base oil.

[0049] Comparative Example 1

[0050] The preparation method of Comparative Example 1 was similar to that of Example 1. The difference from Example 1 was that the ultrasonic conditions used were an ultrasonic intensity of 200 W and an ultrasonic time of 20 min.

[0051] Comparative Example 2

[0052] The difference between the preparation method of Comparative Example 2 and Example 1 was that no surfactant was added.

[0053] Comparative Example 3

[0054] The preparation method of Comparative Example 3 was similar to that of Example 1. The difference from Example 1 was that 1 g of dried olive leaf material was added.

[0055] Comparative Example 4

[0056] The difference between the preparation method of Comparative Example 4 and Example 1 was that the extraction method was magnetic stirring. Stir with a magnetic stirrer at 1000 rpm for 12 h, centrifuge at 10000 rpm for 10 min after stirring, and take the supernatant.

[0057] Test Example 1: Total amount of polyphenolic whitening active ingredients

[0058] 1. Test objects: Cosmetic base oils obtained from Examples 1 to 3 and Comparative Examples 1 to 4.

[0059] 2. Test method: Weigh 1 g of the cosmetic base oils obtained from Examples 1 to 3 and Comparative Examples 1 to 4 respectively, add them to 1 mL of n-hexane and 1 mL of methanol aqueous solution (80:20, V / V), vortex for 3 min, centrifuge for 5 min, take the supernatant, repeat the extraction twice, wash with n-hexane to obtain 3 mL of extract. Then determine the total content by the Folin-Ciocalteu method, and the final result is expressed as mg gallic acid equivalent (mg GAE) / kg oil.

[0060] In addition, the polyphenol contents in different vegetable oils were collected by literature review and compared with Example 1. The polyphenolic whitening active ingredients in the extract obtained by extracting the dried olive leaf material with the traditional organic solvent extraction method (60% methanol water) and the novel cosmetic base oil obtained in Example 1 were compared by UPLC-MS analysis. The chromatographic conditions were a C18 chromatographic column (4.6 mm × 250 mm, 5 μm); mobile phase A was 0.1% acetic acid water, B was acetonitrile, and the gradient elution program was: 0.0 - 25.0 min, 95% A, 5% B; 25.0 - 30.0 min, 65% A, 35% B. The flow rate was 1 mL / min, and the injection volume was 10 μL.

[0061] 3. Test results

[0062] The experimental results are shown in Table 1:

[0063] Total analysis of polyphenolic whitening active ingredients in Table 1

[0064]

[0065] As shown in Table 1, the addition of surfactant increased the extraction rate of polyphenolic whitening active ingredients in vegetable oil. The total amount of polyphenolic whitening active ingredients in Example 1 was 420.36 mg GAE / kg, while in Comparative Example 2 without surfactant, the total amount was 25.01 mg GAE / kg. Similarly, the type and addition amount of surfactant had a significant impact on the total amount of polyphenolic whitening active ingredients. When the surfactant was soy lecithin and the addition amount was 1.5 g, the total amount was the highest. When the addition amount of soy lecithin was 0.25 g, the total amount was 100.15 mg GAE / kg. When glyceryl monooleate was used as the surfactant and 2 g was added, the total amount of polyphenolic whitening active ingredients was only 35.08 mg GAE / kg. In addition, when the addition ratio of plant raw materials, extraction method, and ultrasonic conditions were changed, it would also have a significant impact on the total amount of polyphenolic whitening active ingredients. Comparing the novel cosmetic base oil obtained from Example 1 with the highest total amount of polyphenolic whitening active ingredients with the total polyphenol amounts of other vegetable oils, the results were as Figure 1 shown. The polyphenol content of the prepared novel cosmetic base oil was higher than that of other vegetable oils. In the UPLC-MS analysis, the total ion chromatograms of polyphenols in the organic solvent extract and the cosmetic base oil prepared in Example 1 were as Figure 2 shown. Compared with the traditional organic solvent (B), more polyphenol components were detected in the cosmetic base oil (A), indicating that the present invention was suitable for preparing cosmetic base oils rich in polyphenolic whitening active ingredients.

[0066] Test Example 2: Tyrosinase inhibition rate

[0067] 1. Test objects: Cosmetic base oils obtained from Examples 1 to 3 and Comparative Examples 1 to 4.

[0068] 2. Test method: Weigh 2 g of the cosmetic base oils obtained from Examples 1 to 3 and Comparative Examples 1 to 4 respectively, add them to 2 mL of n-hexane and 2 mL of methanol aqueous solution (80:20, V / V), vortex for 3 min, centrifuge for 5 min, take the supernatant, repeat the extraction twice, and wash with n-hexane to obtain 6 mL of extract. The extract was dried by rotary evaporator, resuspended with 1 mL of PBS buffer, centrifuged at 5000 rpm for 5 min, and take the supernatant. Take 40 μL of the sample in a 96-well plate, add 40 μL of tyrosinase solution (310 u / mL) and 80 μL of PBS buffer (pH 6.8), incubate at 25 °C for 10 min, then add 40 μL of L-tyrosine solution (2.5 mM), incubate for 30 min, and measure the absorbance at 475 nm.

[0069] Inhibition rate of tyrosinase activity (%) = (1 - (A 样 - A 空 ) / A 对 ) × 100

[0070] Wherein, A 样 refers to the absorbance of the mixture containing the sample, tyrosinase and L-tyrosine solution; A 空 refers to the absorbance of the mixture containing the sample and tyrosinase; A 对 refers to the absorbance of the mixture without the sample.

[0071] 3. Test results

[0072] The test results are shown in Table 2 as follows:

[0073] Table 2 Tyrosinase inhibition rate

[0074]

[0075] As shown in Table 2, the sample has an inhibitory effect on tyrosinase activity in vitro and is related to the total amount of polyphenolic whitening active ingredients. The more the total amount, the stronger the inhibitory effect on tyrosinase activity.

[0076] Test Example Three: Inhibition of melanogenesis in B16F10 cells

[0077] 1. Test object: The cosmetic base oils obtained in Examples 1 to 3 and Comparative Examples 1 to 4

[0078] 2. Test method: B16F10 cells grow in a 25 cm 2 culture flask containing DMEM complete medium, where the complete medium contains 10% fetal bovine serum and 1% penicillin / streptomycin, and is placed in a humidified incubator at 37 °C with 5% CO2. When the cells grow to 80% density, the cells are collected by trypsin-EDTA digestive solution, the cell concentration is adjusted to 0.5 × 10 5 / mL, and inoculated into a 6-well plate, 2 mL of cell suspension per well. After the cells are incubated overnight until completely adherent, the cell supernatant is discarded, and the complete medium with a sample concentration of 10 mg / mL is added and cultured for 72 h. After the sample treatment, the cells are collected by digestive solution, washed with PBS buffer and centrifuged, and the washing is repeated 2 times to obtain cell precipitate. 500 μL of sodium hydroxide solution containing 10% DMSO is added to the precipitate, and heated at 60 °C for 1 h to fully lyse the cells and dissolve the melanin. After centrifugation, the supernatant is collected, and the absorbance of the supernatant at 450 nm is measured.

[0079] Inhibition rate of melanogenesis % = (1 - A / A0) × 100

[0080] Among them, A refers to the absorbance of the sample solution, and A0 refers to the absorbance of the solution without the sample solution.

[0081] 3. Test results

[0082] The test results are shown in Table 3:

[0083] Table 3 Melanin production inhibition rate

[0084]

[0085] As shown in Table 3, polyphenolic whitening active ingredients can regulate melanin production in B16F10 cells, and with the increase of the concentration of the active ingredient, the inhibitory effect on melanin production is enhanced, which verifies the whitening effect of the prepared new cosmetic base oil.

[0086] Test Example Four: Skin irritation

[0087] 1. Test objects: The cosmetic base oil obtained in Example 1, commercially available camellia seed oil and olive oil for cosmetics.

[0088] 2. Test method: Before the test, the animals were acclimatized in the experimental animal house environment for 3 days. Four New Zealand rabbits were taken, and the hair on both sides of the back spine was removed, and the hair removal area on each side was about 3 cm × 3 cm. After observing for 24 hours, 0.1 g and 0.5 g of the oil sample were applied to the depilated area on one side of a non-damaged rabbit, and the application area was 2.5 cm × 2.5 cm. The skin on the depilated area on the other side was used as a control. It was applied once a day for 14 consecutive days, and the progress of skin erythema and edema was observed every day. To observe the pathological changes in the deep skin tissue, the treated skin was fixed with 4.0% (w / v) paraformaldehyde, embedded in paraffin, sectioned, stained with hematoxylin / eosin, and photographed under an optical microscope to obtain an imaging pathological section.

[0089] 3. Test results

[0090] The potential skin irritation of different topical formulations was evaluated through the dorsal skin irritation experiment of New Zealand rabbits. The appearance of erythema and edema at the application site indicates that the formulation may cause skin irritation. The test results are as Figure 3 shown. Compared with the control area, after 14 days of treatment at low and high doses in the experimental group, there was no swelling and erythema on the back of the animals, and the pathological section results showed that there was no obvious thickening of the epidermal layer and obvious infiltration of inflammatory cells in each experimental group. It shows that the prepared cosmetic base oil has no irritation to the skin of rabbits and can be safely applied to cosmetic formulations.

[0091] The above are only the preferred embodiments of this application, and are not intended to limit this application. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of this application shall be included within the protection scope of this application.

Claims

1. A cosmetic base oil efficiently enriched with whitening active ingredients, characterized in that: The cosmetic base oil includes plant active ingredients and surfactants, wherein the amount of the surfactant is 1% to 5% of the mass of the plant active ingredients; the plant active ingredients include plant raw materials and plant oil liquid in a weight ratio of 1:4 to 1:

10.

2. The cosmetic base oil efficiently enriched with whitening active ingredients according to claim 1, characterized in that: The vegetable oil refers to the virgin oil or refined oil extracted from the fruit, seed or germ of the plant.

3. The cosmetic base oil efficiently enriched with whitening active ingredients according to claim 1, characterized in that: The surfactant refers to a substance having fixed hydrophilic and lipophilic groups, which can be arranged in a directional manner on the surface of a solution and can significantly reduce the surface tension.

4. The cosmetic base oil efficiently enriched with whitening active ingredients according to claim 1, characterized in that: The surfactant is soybean lecithin or glycerol monooleate.

5. The cosmetic base oil efficiently enriched with whitening active ingredients according to claim 1, characterized in that: The plant raw material refers to at least one of Glycyrrhiza glabra and its processing by-products, and olive processing by-products; the olive processing by-products include olive leaves and / or olive pomace.

6. A method for preparing a cosmetic base oil that efficiently enriches whitening active ingredients as claimed in any one of claims 1 to 5, characterized in that: The following steps are involved: The vegetable oil is mixed with the surfactant, heated and stirred until the surfactant is completely dissolved, and then the plant raw material powder is added. The physical field enhancement technology is used for wall-breaking extraction, and the supernatant is collected by centrifugation to obtain a cosmetic base oil that is efficiently enriched with whitening active ingredients.

7. The method for preparing a cosmetic base oil efficiently enriched with whitening active ingredients according to claim 6, characterized in that: The plant raw material powder refers to the plant raw material powder obtained by drying fresh plant leaves in an oven at 40° C., crushing the dried leaves with a grinder and passing through a 60-mesh standard sieve.

8. The cosmetic base oil efficiently enriched with whitening active ingredients according to claim 6, characterized in that: The stirring temperature of the heating stirring is 30-60° C., the stirring speed is 400-1000 r / min, and the stirring time is 0.5-12 h.

9. The cosmetic base oil efficiently enriched with whitening active ingredients according to claim 6, characterized in that: The added amount of the surfactant is 1% to 5%, and the ratio of the plant raw material to the plant oil is 1:4 to 1:

10.

10. The cosmetic base oil efficiently enriched with whitening active ingredients according to claim 6, characterized in that: The physical field enhancement technology includes ultrasonic technology or microwave technology. When the physical field enhancement technology is ultrasonic technology, the conditions for wall-breaking extraction are ultrasonic power of 300 to 500 W and processing time of 20 to 55 minutes.

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