Preparation method and application of fructus evodiae extract carbon dots capable of resisting aging and rapidly detecting pH and performing cell fluorescence imaging
By using Evergosa extract as a carbon source and synthesized by one-step hydrothermal method, the carbon dots of Evergosa extract in the prior art were solved, and the problem of insufficient multifunctionality of anti-aging, rapid detection of pH and cell fluorescence imaging in the prior art was achieved, and efficient and multifunctional carbon dot materials were achieved.
Patent Information
- Application Number
- CN202510358231.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-25
- Publication Date
- 2025-06-24
AI Technical Summary
Existing carbon dot materials have shortcomings in anti-aging and rapid detection of pH and cell fluorescence imaging, making it difficult to achieve versatility and efficiency.
Evergosa extract was used as the carbon source to synthesize carbon dots of Evergosa extract with anti-aging, rapid pH detection and cell fluorescence imaging through green and simple one-step hydrothermal method.
It realizes the versatility of carbon dots of Evergosa extract, has the ability to anti-aging, rapid detection of pH and cell fluorescence imaging, and has good biocompatibility and water solubility.
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Figure CN120189366A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the field of carbon dots, and specifically relates to a preparation method and application of evodia rutaecarpa extract carbon dots with anti-aging properties and the ability to rapidly detect pH and perform cell fluorescence imaging. Background Art
[0002] Carbon dots (CDs) are a new member of the nanomaterial family, with excellent photostability, good biocompatibility, low toxicity, eco-friendliness, simple green synthesis, and easy surface modification, etc., which have attracted great interest in the biomedical field. The typical size of CDs is less than 10 nm, and the particle size generally distributes between 2 - 8 nm, with an internal sp 2 and external sp 3 hybrid structure, and its lattice spacing is composed of carbon, oxygen, and surface functional groups. These surface functional groups endow CDs with many properties, including hydrophilicity, electron transfer properties, etc. The photoluminescence of CDs also changes with the structure, surface groups, defects, and environment, making its research and application in the fields of bioimaging, biosensing, drug delivery, and treatment develop rapidly. The raw materials for preparing CDs are widely sourced, such as citric acid, polyethyleneimine, glucose, amino acids, etc., and various carbon-containing substances have the potential to be prepared into carbon nanodots.
[0003] In recent years, traditional Chinese medicinal herbs have received extensive attention from researchers because of their wide sources, large yields, and almost no harm. More importantly, Chinese herbal medicines are rich in active ingredients and have multiple pharmacological effects, which not only meet the urgent need for large-scale production but also can be sustainably applied. In previous reports, some Chinese medicines have been used as raw materials for preparing functional CDs due to their unique curative effects. Summary of the Invention
[0004] In order to solve the above problems, the present invention provides a preparation method and application of evodia rutaecarpa extract carbon dots with anti-aging properties and the ability to rapidly detect pH and perform cell fluorescence imaging.
[0005] An application of evodia rutaecarpa extract carbon dots of the present invention, wherein the evodia rutaecarpa extract carbon dots are used for preparing anti-aging drugs.
[0006] Further, the mass concentration of the evodia rutaecarpa extract carbon dots is 25 - 100 μg / mL.
[0007] An application of evodia rutaecarpa extract carbon dots of the present invention, wherein the evodia rutaecarpa extract carbon dots are used for preparing pH detection preparations or reagents for cell fluorescence imaging.
[0008] Further, the mass concentration of the evodia rutaecarpa extract carbon dots is 25 - 100 μg / mL.
[0009] A kit containing the preparation for detecting pH or the reagent for cell fluorescence imaging of the present invention.
[0010] A preparation method of evodia rutaecarpa extract carbon dots with anti-aging effect and capable of rapidly detecting pH and performing cell fluorescence imaging according to the present invention, which is carried out as follows:
[0011] Take the evodia rutaecarpa extract, add water and stir to mix evenly, then transfer it to a polytetrafluoroethylene-lined autoclave. Under the condition of a temperature of 160 - 180 °C, react for 7 - 10 h. After cooling, filter to obtain the evodia rutaecarpa extract carbon dots with anti-aging effect and capable of rapidly detecting pH and performing cell fluorescence imaging.
[0012] Furthermore, the preparation method of the evodia rutaecarpa extract is as follows:
[0013] Take evodia rutaecarpa fruits, wash, remove impurities, crush and sieve to obtain evodia rutaecarpa powder; dissolve it in water, and add ethanol with a volume percentage content of 70% for extraction according to the ratio of solid to liquid of 1:10. After extraction, concentrate and dry to obtain the evodia rutaecarpa extract; among them, the extraction conditions are extracting 3 times at room temperature for 4 hours each time.
[0014] Furthermore, the mass concentration of the prepared evodia rutaecarpa extract carbon dots with anti-aging effect and capable of rapidly detecting pH and performing cell fluorescence imaging is 25 - 100 μg / mL.
[0015] The present invention has the following beneficial effects:
[0016] The present invention uses the traditional Chinese medicine - evodia rutaecarpa as the precursor for the development of functionalized CDs, uses the evodia rutaecarpa extract as the carbon source, and synthesizes ultra-small-sized evodia rutaecarpa extract carbon nanodots by a green and simple one-step hydrothermal method. The evodia rutaecarpa extract carbon dots have a uniform particle size distribution and rich surface groups, have good water solubility and biocompatibility, and can achieve the purposes of efficient skin whitening and anti-aging through the effects of antioxidant and inhibiting melanin synthesis; at the same time, because of the fluorescence characteristics of the carbon dots, they can be used as fluorescence probes to rapidly detect pH and can enter cells for cell fluorescence imaging. Description of the Drawings
[0017] Figure 1 TEM image of the prepared evodia rutaecarpa extract carbon dots;
[0018] Figure 2 Particle size distribution diagram of the evodia rutaecarpa extract carbon dots;
[0019] Figure 3 Fluorescence spectrum diagram of the evodia rutaecarpa extract carbon dots under different excitation wavelengths;
[0020] Figure 4UV-Vis absorption spectra of Evodia rutaecarpa extract and carbon dots of Evodia rutaecarpa extract (Illustration: Photos of carbon dots of Evodia rutaecarpa extract under daylight lamp (left) and 365 nm UV lamp (right));
[0021] Figure 5 Infrared spectrum of carbon dots of Evodia rutaecarpa extract;
[0022] Figure 6 X-ray photoelectron spectroscopy of carbon dots of Evodia rutaecarpa extract;
[0023] Figure 7 Schematic diagram of the peak fitting results of C1s of carbon dots of Evodia rutaecarpa extract;
[0024] Figure 8 Schematic diagram of the peak fitting results of O1s of carbon dots of Evodia rutaecarpa extract;
[0025] Figure 9 Fluorescence decay curve of carbon dots of Evodia rutaecarpa extract;
[0026] Figure 10 Relationship between the fluorescence intensity of carbon dots of Evodia rutaecarpa extract and different pH values;
[0027] Figure 11 Fluorescence intensity diagram of carbon dots of Evodia rutaecarpa extract under the alternating conditions of pH = 3 and pH = 10 with cyclic switching;
[0028] Figure 12 Diagram of the change in the viability of Hacat cells treated with carbon dots of Evodia rutaecarpa extract;
[0029] Figure 13 Diagram of the change in the viability of B16-F10 cells treated with carbon dots of Evodia rutaecarpa extract;
[0030] Figure 14 Fluorescence microscope image of carbon dots of Evodia rutaecarpa extract and cells after 6 h. Detailed implementation mode
[0031] To make the objectives, technical solutions and advantages of the embodiments of the present invention clearer and more understandable, the spirit of the content disclosed in the present invention will be described in detail. After any person skilled in the art in the technical field to which the present invention pertains understands the embodiments of the content of the present invention, they can make changes and modifications based on the technology taught by the content of the present invention, and it does not deviate from the spirit and scope of the content of the present invention.
[0032] The illustrative embodiments of the present invention and their descriptions are used to explain the present invention, but do not limit the present invention.
[0033] Example 1 Preparation of carbon dots of Evodia rutaecarpa extract
[0034] The carbon dots of Evodia rutaecarpa extract were prepared by a one-step hydrothermal synthesis method. Precisely weigh 30 mg of the Evodia rutaecarpa extract powder, place it in a beaker containing 10 mL of distilled water, add a magnetic stir bar, and stir on a magnetic stirrer for 10 min to fully dissolve the Evodia rutaecarpa extract powder, making the solution concentration 3.0 mg / mL. Then transfer the solution to a polytetrafluoroethylene-lined autoclave (15 mL) and heat it in an oven at 170 °C for 8 h. After the reaction time is reached, place the autoclave in cold water and cool it rapidly (20 min). Suction out the solution in the liner, and perform rough filtration using a 0.22 μm polyethersulfone membrane to remove larger aggregates and precipitates, obtaining the corresponding pale yellow carbon dot solution of Evodia rutaecarpa extract, and store it at 4 °C.
[0035] 1. Physicochemical property characterization of carbon dots of Evodia rutaecarpa extract
[0036] Instruments: Transmission electron microscope (TEM) images were obtained using a Tecnai F20 electron microscope; ultraviolet-visible absorption spectra were obtained using a UV-2550 ultraviolet-visible spectrophotometer; fluorescence spectra were obtained using an F-2700 fluorescence spectrophotometer; infrared spectra were obtained using a Vector33 Fourier transform infrared spectrometer; fluorescence lifetime and quantum yield were obtained using an FLS 920 steady-state / transient fluorescence spectrometer; X-ray photoelectron spectra were obtained using an ESCALAB 250 X-ray photoelectron spectrometer.
[0037] Experimental results: The prepared carbon dots of Evodia rutaecarpa extract had a particle size concentrated at 2.86 ± 0.65 nm, with a uniform size distribution ( Figure 1-2 ), and had obvious excitation-dependent characteristics. The optimal excitation wavelength was at 370 nm, and the optimal emission wavelength was 438 nm ( Figure 3 ). The obtained carbon dots of Evodia rutaecarpa extract were a pale yellow liquid and had strong blue-green fluorescence under irradiation with a 365 nm ultraviolet lamp. As Figure 4 shown in the ultraviolet absorption spectrum, the Evodia rutaecarpa extract solution had ultraviolet absorption peaks at 274 nm and 314 nm, while the carbon dot solution of Evodia rutaecarpa extract had only one ultraviolet absorption peak at 278 nm. As Figure 5 shown in the infrared spectrum diagram, the carbon dots of Evodia rutaecarpa extract had absorption bonds at 3380, 2930, 2871, 1610, 1100 - 1000 cm-1, which were attributed to the vibrations and rotations of O-H, C-H, C=O, and C-O. X-ray photoelectron spectroscopy (XPS) was used to further characterize and analyze the elemental composition and functional groups of the carbon dots of Evodia rutaecarpa extract. As Figures 6-8As shown, two main peaks appeared in the XPS spectrum of Evodia rutaecarpa extract carbon dots at 284.5 and 532.4 eV, corresponding to the C1s and O1s elements respectively; the C 1s band had three peaks at 287.9, 285.5 and 284.0 eV, which belonged to the carbon peaks related to C=O, C-O, C-C / C=C respectively; the O 1s band had a peak between 528 - 535 eV, related to C=O and C-O-H / C-O-C. We also used a steady-state / transient fluorescence spectrometer to measure the fluorescence lifetime and quantum yield of Evodia rutaecarpa extract carbon dots. Fitting with a second-order decay exponential function gave 2 fluorescence lifetimes (τ), indicating that GS-CDs had 2 fluorescence centers, with different radiative transition processes for luminescence, and there might be luminescence mechanisms such as surface states and edge states (including band-edge luminescence), carbon core states and molecular states. The average fluorescence lifetime of Evodia rutaecarpa extract carbon dots was 2.70 ns, and the quantum yield was 34.81%( Figure 9 ).
[0038] 2. Fluorescent response of Evodia rutaecarpa extract carbon dots to pH
[0039] To adjust the pH of Evodia rutaecarpa extract carbon dots (concentration 100 μg / mL), the Evodia rutaecarpa extract carbon dot solution was treated with sodium hydroxide (0.1 M) or hydrochloric acid (0.1 M) solution, and the final pH values were 3.2, 4.5, 5.7, 6.8, 8.1, 9.3 and 10.4 respectively. Subsequently, the changes in fluorescence intensity with the best excitation wavelength at 370 nm and the best emission wavelength at 438 nm were recorded.
[0040] Experimental results: As Figure 10 shown in Figure 11, Evodia rutaecarpa extract carbon dots can be used as a fluorescent probe for rapid pH detection.
[0041] 3. Study the cytotoxicity of Evodia rutaecarpa extract carbon dots
[0042] In this example, the Cell Counting Kit-8 method was used to detect the changes in cell viability after evodia extract carbon dots acted on human immortalized epidermal Hacat cells (RPMI-1640 medium) and mouse melanoma B16-F10 cells (DMEM medium) for 48 h. Cells in the logarithmic growth phase were taken, digested and resuspended, and then seeded into 96-well plates. The volume of cell suspension in each well was 90 μL, and they were cultured in a cell incubator for about 6 - 12 h. After the cells were completely adherent, 10 μL of evodia extract carbon dot solutions with different concentrations were added, and the final concentrations acting on the cells in each well were 0, 25, 50, 75, and 100 μg / mL (6 replicates were set for each concentration), and then they were continued to be cultured in the incubator. After 48 h of co-incubation of evodia extract carbon dots and cells, 10 μL of CCK-8 was added to each well and reacted in the incubator for about 1 h, and the optical density (OD) at 450 nm was measured with an enzyme-linked immunosorbent assay (ELISA) reader. The cell viability was calculated based on the OD values of the experimental group and the control group.
[0043] Experimental results: As Figure 12 shown, evodia extract carbon dots had almost no inhibitory effect on Hacat cells, indicating its good biocompatibility and negligible toxic and side effects. As Figure 13 shown, evodia extract carbon dots could significantly inhibit the growth of mouse melanoma B16-F10 cells.
[0044] 4. Cellular fluorescence imaging of evodia extract carbon dots
[0045] In this example, a fluorescence inverted microscope was used to take cellular fluorescence imaging pictures of the uptake of evodia extract carbon dots in cells. A round microscope coverslip was placed in a 6-well cell culture plate, and 2 mL of cell suspension, 1×10 5 cells / well, was added and cultured in the incubator for 24 h to enable the cells to adhere to the coverslip and grow stably. The supernatant medium was aspirated, and the medium containing evodia extract carbon dots was used to replace it, with a final concentration of 100 μg / mL. After co-incubation with the cells for 6 h, the supernatant was aspirated, and the cells were carefully washed 3 times with PBS without blowing down the adherent cells. Next, 1 mL of 4% paraformaldehyde solution was added to fix the cell morphology for 15 min, and the cells were carefully washed 3 times with PBS. A glass slide was prepared, and 10 μL of anti-fluorescence quenching mounting medium was dropped on the slide. The side with cells was buckled on the anti-fluorescence quenching mounting medium on the slide to make a cell smear, which was stored in the dark and could be used for taking cellular fluorescence imaging pictures.
[0046] Experimental results: As Figure 14 shown, evodia extract carbon dots could be used as a cellular fluorescence probe to observe and track their entry into cells.
[0047] 5. Human safety test (human patch test)
[0048] The human skin closed patch test was carried out on the evodia rutaecarpa extract carbon dot solution to evaluate its potential skin irritation. The test method referred to the human skin patch test in the "Technical Specifications for Cosmetics Safety" (2015 edition). Thirty volunteers aged between 18 and 60 years old were selected as the test subjects. Several layers of gauze were cut into about 1 cm 2 in size. 50 μL of the 100 μg / mL evodia rutaecarpa extract carbon dot solution was dropped onto the gauze, and it was applied to the inner forearm skin of the volunteers. A layer of plastic film was covered on the outside to make it evenly adhere to the skin, and it was fixed with a bandage for 24 h. The negative control was a blank control (without placing any substance). At 30 min (after the indentation disappeared), 24 h, and 48 h after removing the patch tester, the local skin reactions were observed according to the standard in Table 2, and the observation results were recorded. The patch test results are shown in Table 2.
[0049] As can be seen from Table 2, the test results were all negative reactions, and no adverse reactions occurred in the human skin of the subjects, indicating that the evodia rutaecarpa extract carbon dots are safe for humans and have no irritant reactions.
[0050] Table 1 Grading criteria for skin reactions in the skin closed patch test
[0051]
[0052] Table 2 Human safety test results
[0053]
[0054]
[0055] 6. Antioxidant activity detection
[0056] 6.1 Determination of DPPH free radical scavenging ability
[0057] Weigh 4 mg of DPPH and place it in a 100 mL brown volumetric flask. Add absolute ethanol to the scale and shake well to obtain a DPPH solution with a mass concentration of 0.04 mg / mL. Take 1 mL of the evodia rutaecarpa extract carbon dot solution with different concentrations (0.025, 0.050, 0.075, 0.100 mg / mL) respectively, add 1 mL of the DPPH solution, mix well, and let it stand at room temperature for 30 min. Then centrifuge at 5000 r / min for 10 min. Take the supernatant and measure the absorbance at 517 nm. The DPPH scavenging rate is calculated according to the formula: DPPH scavenging rate (%) = [1 - (A i - A j ) / A c × 100%.
[0058] Ac — Absorbance value of 1 mL absolute ethanol + 1 mL DPPH solution;
[0059] A i — Absorbance value of 1 mL sample solution + 1 mL DPPH solution;
[0060] A j — Absorbance value of 1 mL sample solution + 1 mL absolute ethanol.
[0061] The experimental results are shown in Table 3. The carbon dots of Evodia rutaecarpa extract have strong scavenging ability against DPPH free radicals.
[0062] Table 3 Scavenging rate of carbon dots of Evodia rutaecarpa extract against DPPH free radicals
[0063]
[0064] 6.2 Determination of hydroxyl radical scavenging ability
[0065] Add 0.5 mL of 9 mmol / L FeSO4, 0.5 mL of 9 mmol / L salicylic acid - ethanol solution, 2.5 mL of Evodia rutaecarpa extract carbon dot solution with different concentrations (0.025, 0.050, 0.075, 0.100 mg / mL) and 0.5 mL of 8.8 mmol / L H2O2 solution to the reaction system in sequence, shake well, heat in a water bath at 37 °C for 15 min, and measure the absorbance at 510 nm.
[0066] The hydroxyl radical scavenging rate is calculated according to the formula: ·OH scavenging rate (%) = [1 - (A i - A j ) / A c × 100%.
[0067] A c is the absorbance value of 2% ethanol solution instead of the sample solution,
[0068] A i is the absorbance value of adding the sample solution;
[0069] A j is the absorbance value of using distilled water instead of H2O2 solution.
[0070] The experimental results are shown in Table 4. The carbon dots of Evodia rutaecarpa extract have strong scavenging ability against hydroxyl radicals.
[0071] Table 4 Scavenging rate of carbon dots of Evodia rutaecarpa extract against hydroxyl radicals
[0072]
[0073]
[0074] 7. Whitening Efficacy Detection
[0075] 7.1 Tyrosinase Experiment:
[0076] Solution Preparation: Prepare 1 / 15 mol / L Na2HPO4-KH2PO4 buffer solution (pH 6.8); Take 25000 u tyrosinase and make it into a 1000 u / mL solution with phosphate buffer solution, and store it frozen at -20 °C; Weigh DL-dopa and make it into a 0.15% solution with phosphate buffer solution; Accurately weigh arbutin and make it into an arbutin control solution of 15.00 mg / mL with phosphate buffer solution; Carbon dot solutions of evodia rutaecarpa extract at different concentrations (0.025, 0.050, 0.075, 0.100 mg / mL).
[0077] Experimental Procedure: In a 24-well ELISA plate, add 30 μL (30 u) of 1000 u / mL tyrosinase solution to each well, then add 250 μL of arbutin control solution or carbon dot solutions of evodia rutaecarpa extract at different concentrations respectively, incubate at 37 °C for 10 min, add 500 μL of 0.15% DL-dopa, continue to incubate at 37 °C for 5 min, and immediately measure the absorbance at 490 nm with an ELISA reader. The tyrosinase inhibition rate is calculated according to the formula: Tyrosinase inhibition rate (%) = {[(A - B) - (C - D)] / (A - B)} × 100%.
[0078] A is the absorbance value of adding 250 μL of buffer solution and 30 u of tyrosinase;
[0079] B is the absorbance value of adding only 280 μL of buffer solution;
[0080] C is the absorbance value of adding 250 μL of the medicinal solution and 30 u of tyrosinase;
[0081] D is the absorbance value of adding 250 μL of the medicinal solution and 30 μL of buffer solution.
[0082] The experimental results are shown in Table 5, indicating that the carbon dots of evodia rutaecarpa extract have the effect of inhibiting tyrosinase, and thus have the whitening efficacy.
[0083] Table 5 Inhibition Rate of Carbon Dots of Evodia Rutaecarpa Extract on Tyrosinase
[0084]
[0085] 7.2 Experiment on Inhibiting Melanin Synthesis in Melanocytes
[0086] The steps of culturing B16-F10 cells are the same as those in 4.
[0087] Take cells in the logarithmic growth phase, dilute them to an appropriate density and culture them in 5 wells of a 6-well cell culture plate. After culturing in a cell incubator for 24 h, add only 3 mL of cell culture medium to the first well as a control group. Add different concentrations of evodia rutaecarpa extract carbon dots solution (3 mL in total) diluted with the medium to the other 4 wells, so that the final concentrations acting on the cells in each well are 25, 50, 75, and 100 μg / mL respectively, and continue to culture in the incubator. After co-incubating evodia rutaecarpa extract carbon dots with the cells for 48 h, discard the supernatant, wash twice with PBS, digest and centrifuge each component, resuspend with 0.5 mL of PBS, and take 50 μL for cell counting. Centrifuge the remaining cell suspension again, remove the supernatant, add NaOH solution to the precipitate, heat to dissolve the melanin, and immediately measure the absorbance at 490 nm with an enzyme-labeled instrument. The melanin inhibition rate is calculated according to the formula: Melanin inhibition rate (%) = [1 - (A1÷P1) / (A2÷P2)]×100%.
[0088] A1 is the absorbance value of the evodia rutaecarpa extract carbon dots group;
[0089] P1 is the cell density of the evodia rutaecarpa extract carbon dots group;
[0090] A2 is the absorbance value of the control group;
[0091] P2 is the cell density of the control group.
[0092] The experimental results are shown in Table 6, indicating that evodia rutaecarpa extract carbon dots have the effect of inhibiting melanin synthesis, and thus have the efficacy of whitening.
[0093] Table 6 Inhibition rate of evodia rutaecarpa extract carbon dots on melanin
[0094]
Claims
1. An application of Evodia rutaecarpa extract carbon dots, characterized in that The Evodia rutaecarpa extract carbon dots are used for preparing anti-aging drugs.
2. The use of the Evodia rutaecarpa extract carbon dots according to claim 1, characterized in that The mass concentration of the Evodia rutaecarpa extract carbon dots is 25-100 μg / mL.
3. An application of Evodia rutaecarpa extract carbon dots, characterized in that The Evodia rutaecarpa extract carbon dots are used to prepare a preparation for detecting pH, or to prepare a reagent for cell fluorescence imaging.
4. The use of the Evodia rutaecarpa extract carbon dots according to claim 1, characterized in that The mass concentration of the Evodia rutaecarpa extract carbon dots is 25-100 μg / mL.
5. A kit comprising the preparation for detecting pH or the reagent for cell fluorescence imaging according to claim 3 or 4.
6. A method for preparing carbon dots from Evodia rutaecarpa extract having anti-aging properties and capable of rapid pH detection and cell fluorescence imaging, characterized in that It is carried out as follows: Take the Evodia rutaecarpa extract, add water and stir to mix evenly, then transfer to a polytetrafluoroethylene-lined high-pressure reactor, react for 7 to 10 hours at a temperature of 160 to 180° C., cool, and filter to obtain the Evodia rutaecarpa extract carbon dots that have anti-aging properties and can quickly detect pH and perform cell fluorescence imaging.
7. The method for preparing the carbon dots of Evodia rutaecarpa extract having anti-aging effect and capable of rapid pH detection and cell fluorescence imaging according to claim 6, characterized in that The preparation method of the Evodia rutaecarpa extract is as follows: Take Evodia rutaecarpa fruit, wash, remove impurities, crush and sieve to obtain Evodia rutaecarpa powder; add water to dissolve, add 70% ethanol by volume in a ratio of 1:10 between solid and liquid, extract, concentrate and dry to obtain Evodia rutaecarpa extract; wherein the extraction condition is 3 times at room temperature, each time for 4 hours.
8. The method for preparing the carbon dots of Evodia rutaecarpa extract having anti-aging effect and capable of rapid pH detection and cell fluorescence imaging according to claim 6, characterized in that The mass concentration of the prepared Evodia rutaecarpa extract carbon dots, which have anti-aging effects and can quickly detect pH and perform cell fluorescence imaging, is 25-100 μg / mL.