An anti-aging repair composition containing algae and its application

Abstract

The present application provides an algae-containing anti-aging repair composition and its application, wherein the anti-aging repair composition includes cyanobacteria, tripeptides and bacillus fermentation products, and the mass ratio of the cyanobacteria, tripeptides and bacillus fermentation products is 0.01~0.5:0.1~1:0.2~2. The composition obtained by compounding cyanobacteria, tripeptides and bacillus fermentation products in the present application has a synergistic effect in anti-aging, especially in promoting collagen secretion. When cyanobacteria is replaced by other algae or bacillus fermentation products are replaced by other fungal fermentation products, the efficacy of the composition disclosed in the present application cannot be achieved. At the same time, the composition provided by the present invention also has multiple functions such as barrier repair, anti-oxidation, anti-inflammatory, promotion of cell-to-cell interaction, and promotion of collagen synthesis and inhibition of collagen degradation.

Description

Technical Field

[0001] The present invention relates to the technical field of daily chemical products, and in particular to an algae-containing anti-aging and repairing composition and application thereof. Background Art

[0002] Aging is categorized into intrinsic and extrinsic causes, with photoaging being the most important exogenous form. Numerous anti-aging products have been developed, employing ingredients such as acetyl hexapeptide-8, which has anti-wrinkle properties, peptides that promote collagen synthesis, and phosphatidylcholine, which promotes the production of the extracellular matrix, to achieve these effects.

[0003] In addition to anti-aging, whether the product has repair and other effects is also a concern of consumers. Summary of the Invention

[0004] In view of this, the technical problem to be solved by the present invention is to provide an anti-aging and repairing composition containing algae and its application, wherein the composition has good anti-aging and repairing effects.

[0005] The present application provides an algae-containing anti-aging repair composition, comprising cyanobacteria, a tripeptide and a Bacillus fermentation product, wherein the mass ratio of the cyanobacteria, the tripeptide and the Bacillus fermentation product is 0.01~0.5:0.1~1:0.2~2.

[0006] This application uses a combination of cyanobacteria, tripeptides and Bacillus fermentation products, which produce a synergistic effect and have good anti-aging, repair, antioxidant, anti-inflammatory, promotion of intercellular interaction, and promotion of collagen synthesis and inhibition of collagen degradation.

[0007] Cyanobacteria, also known as CYANOBACTERIA, can mimic the mechanisms of action of endogenous growth factors, such as vascular endothelial growth factor (VEGF), promoting epidermal repair and dermal microvascular repair. This application does not specifically limit the cyanobacteria described; commercially available cyanobacteria that can be used as a cosmetic ingredient are sufficient. In some specific implementations, the cyanobacteria can be a pulverized product of Phormidium uncinatum, with accession number CCAP1462 / 7.

[0008] In some specific implementations, the cyanobacteria can be prepared according to the following method:

[0009] (1) Cultivating cyanobacteria under white light illumination;

[0010] (2) crushing the cultured cyanobacterial biomass and filtering it to obtain a supernatant;

[0011] (3) The supernatant is mixed with α-hydroxy acid salt and maltodextrin, and the mixture is homogenized, filtered, and dried to obtain cyanobacteria powder.

[0012] Specifically, the present application first sows cyanobacteria in the exponential growth stage and cultures them at 25-26° C. under white light illumination, preferably for 10-20 days, to obtain cyanobacterial biomass.

[0013] After culturing to obtain the cyanobacterial biomass, it is filtered, washed, and then crushed. The crushed cyanobacterial biomass is filtered again to obtain a supernatant. Specifically, the present application first filters the cyanobacterial biomass through a 50 μm to 70 μm, preferably 60 μm, sieve, then washes it in water, crushes it under a pressure of 200 bar to 500 bar, preferably 300 bar to 400 bar, and then filters the crushed biomass through a 0.1 μm to 0.5 μm, preferably 0.2 μm to 0.3 μm membrane to obtain a supernatant.

[0014] After obtaining the supernatant, it is mixed with an α-hydroxy acid salt and maltodextrin, homogenized and filtered again, and the supernatant is dried to obtain cyanobacteria powder. Specifically, the α-hydroxy acid salt can be a lactate, such as calcium lactate or magnesium lactate, preferably a combination of calcium lactate and magnesium lactate, and its mass content in the cyanobacteria powder is 0.5% to 1% and 1% to 1.5%, respectively. The mass content of the maltodextrin in the cyanobacteria powder is 5% to 7%. The supernatant is mixed with the α-hydroxy acid salt and maltodextrin, homogenized and filtered again through a 0.1μm to 0.5μm filter, preferably a 0.2μm to 0.3μm filter, and the pH value of the obtained supernatant is adjusted to 6 to 7, preferably 6.5, and the supernatant is dried to obtain cyanobacteria powder.

[0015] Tripeptides, especially palmitoyl-modified tripeptides, mainly act on the dermis, promote the production of extracellular matrix of fibroblasts, such as collagen, glycosaminoglycans, etc., and inhibit collagen degradation, which has a good effect on improving skin aging caused by photoaging, such as collagen loss and damage. The present application has no special restrictions on the tripeptide, and preferably a palmitoyl-modified tripeptide, such as palmitoyl tripeptide-5. Palmitoyl tripeptide-5, the English name is Palmitoyl Tripeptide-5, also written as Pal-KTTKS, CAS No. 623172-55-4, promotes collagen production by activating cell signaling pathways, significantly reduces wrinkles, improves skin firmness and radiance, and shows potential in wound healing and tissue repair. The present application has no special restrictions on the source of the palmitoyl tripeptide-5, and it can be purchased on the market.

[0016] Skin stem cells are the basis for maintaining skin function and repairing tissue damage, and the endogenous stress factor cortisol will accelerate the damage to skin stem cells. Bacillus fermentation products can reduce the stem cell damage caused by cortisol, thereby maintaining skin function and repairing tissue damage. This application has no special restrictions on the Bacillus fermentation products, and any cosmetic raw materials purchased on the market can be used. In some specific implementations, the Bacillus fermentation product contains active ingredients such as γ-polyglutamic acid and / or metabolic small molecule polyamines, and the total content of the active ingredients is not less than 0.1%.

[0017] In some specific implementations, the mass ratio of the cyanobacteria, tripeptide and Bacillus fermentation product is 0.01-0.25:0.1-0.6:0.2-1. In some specific implementations, the mass ratio of the cyanobacteria, tripeptide and Bacillus fermentation product is 0.05-0.25:0.6:1. In some specific implementations, the mass ratio of the cyanobacteria, tripeptide and Bacillus fermentation product is 0.25:0.6:1. In some specific implementations, the mass ratio of the cyanobacteria, tripeptide and Bacillus fermentation product is 0.01:0.13:0.22. In some specific implementations, the mass ratio of the cyanobacteria, tripeptide and Bacillus fermentation product is 0.05:0.6:1.

[0018] The composition obtained by compounding cyanobacteria, tripeptides and bacillus fermentation products in the present application has a synergistic effect in anti-aging, especially in promoting collagen secretion. However, when cyanobacteria is replaced by other algae or bacillus fermentation products are replaced by other fungal fermentation products, the efficacy of the composition disclosed in the present application cannot be achieved. Specifically, the composition provided by the present application can significantly increase the secretion of type I collagen. In addition, the composition obtained by compounding cyanobacteria, tripeptides and bacillus fermentation products in the present application can promote cell migration and has the effect of repairing skin. The composition provided by the present application can significantly increase the expression of genes related to keratinocyte differentiation and barrier-related pathways, antioxidant / inflammation inhibition and cell interaction, and the increase is greater than 150, with statistical difference, much higher than the comparative example group. At the same time, the extracellular matrix metalloproteinase MMP3 is significantly reduced. The composition provided by the present application can significantly increase the expression of human primary fibroblast growth factor and cell-related genes, and the increase is greater than 150, with statistical difference, much higher than the comparative example group. Therefore, the composition provided by the present invention has multiple functions such as barrier repair, anti-oxidation, anti-inflammation, upregulation of cell-to-cell interactions such as integrin and growth factor to promote healing (scratch test) and repair.

[0019] The present invention also provides a product, comprising: the anti-aging repair composition described in the above technical solution.

[0020] In some embodiments of the present invention, the above products include but are not limited to personal care products, medicines, etc. Those skilled in the art will appreciate that, in addition to the composition described in the above technical solution, other excipients may also be included, such as pharmaceutical excipients or excipients for personal care products.

[0021] In some embodiments of the present invention, the personal care product includes: a basic care product and / or a makeup product.

[0022] In some embodiments of the present invention, the cosmetic products include but are not limited to:

[0023] (1) Foundation makeup:

[0024] Liquid foundation / cream: used to even out skin tone and cover blemishes;

[0025] BB Cream / CC Cream: A lightweight makeup base that combines skincare and finishing touches.

[0026] Concealer / pen: for covering local areas such as acne, dark circles, etc.

[0027] Loose powder / face powder: sets makeup and reduces facial shine.

[0028] (2) Eye makeup:

[0029] Eyeshadow: adds color and depth to the eyes;

[0030] Eyeliner pencil / liquid / gel: outline the eyeliner to make the eyes look more vivid;

[0031] Mascara: lengthens and thickens eyelashes, adds depth to eyes;

[0032] Eyebrow pencil / eyebrow powder / eyebrow gel: fill in the gaps in the eyebrows and shape the ideal eyebrows.

[0033] (3) Cheek makeup:

[0034] Blush: Add natural redness to cheeks and enhance complexion;

[0035] Contouring cake / stick: Use shading techniques to make the facial contours more three-dimensional;

[0036] (4) Lip makeup:

[0037] Lipstick / Lip Glaze / Lip Gloss: Change or emphasize the color of the lips;

[0038] Lip liner: Draw a clear lip boundary to prevent lipstick from overflowing.

[0039] (5) Multifunctional cosmetics:

[0040] Highlighter stick / liquid / powder: Brighten the high points of the face (such as the bridge of the nose and cheekbones) to create a radiant effect.

[0041] In addition, there are products designed specifically for special occasions, such as waterproof and sweat-proof eyeliner and long-lasting lipstick.

[0042] In some embodiments of the present invention, the basic care products include but are not limited to:

[0043] Facial Cleanser / Face Wash: Gently removes dirt, oil and makeup residue from the face;

[0044] Makeup remover oil / makeup remover water / makeup remover cream: specially designed to completely remove makeup, especially waterproof makeup;

[0045] Toner / Lotion: Use after cleansing to further cleanse residue on the skin surface, replenish moisture to the skin, restore the skin's pH balance, and lay a good foundation for the absorption of subsequent skin care products;

[0046] Essence: Contains high concentrations of active ingredients, providing deep nourishment and repair for specific skin problems (such as anti-aging, moisturizing, whitening, etc.);

[0047] Eye cream: specially designed for the sensitive area around the eyes, it can reduce fine lines, dark circles and tighten the skin around the eyes. The texture is usually light and easy to absorb.

[0048] Day / night lotion or cream: has the functions of protecting, repairing and nourishing, promoting cell regeneration, etc., which can provide the skin with necessary moisture and lock in the replenished moisture;

[0049] Sunscreen: used for UV protection, prevention of photoaging, etc.;

[0050] Facial masks: Provide extra nourishment and care to the skin, such as hydration, pore cleansing or brightening.

[0051] This application uses a combination of cyanobacteria, tripeptides and Bacillus fermentation products, which produce a synergistic effect and have good anti-aging, repair, antioxidant, anti-inflammatory and other effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0052] Figure 1 This is a statistical chart of in vitro type I collagen test results;

[0053] Figure 2 The cell scratch test results provided for Experimental Example 2. DETAILED DESCRIPTION

[0054] The present invention provides an algae-containing anti-aging and repairing composition and its application. Those skilled in the art can refer to the disclosure herein and appropriately modify the process parameters to achieve the desired effect. The methods and applications of the present invention have been described through preferred embodiments. It is apparent that those skilled in the art can modify, alter, and combine the methods and applications herein to implement and apply the technology of the present invention without departing from the disclosure, spirit, and scope of the present invention.

[0055] The present application provides an algae-containing anti-aging repair composition, comprising cyanobacteria, a tripeptide and a Bacillus fermentation product, wherein the mass ratio of the cyanobacteria, the tripeptide and the Bacillus fermentation product is 0.01~0.5:0.1~1:0.2~2.

[0056] This application uses a combination of cyanobacteria, tripeptides and Bacillus fermentation products, which produce a synergistic effect and have good anti-aging, repair, antioxidant, anti-inflammatory, promotion of intercellular interaction, and promotion of collagen synthesis and inhibition of collagen degradation.

[0057] The composition obtained by compounding cyanobacteria, tripeptides and Bacillus fermentation products in this application has a synergistic effect in anti-aging, especially in promoting collagen secretion. However, when cyanobacteria is replaced by other algae or Bacillus fermentation products are replaced by other fungal fermentation products, the efficacy of the composition disclosed in this application cannot be achieved. Specifically, the composition provided by this application can significantly increase the secretion of type I collagen. In addition, the composition obtained by compounding cyanobacteria, tripeptides and Bacillus fermentation products in this application can promote cell migration and have the effect of repairing skin. The composition provided by this application can significantly increase the expression of genes related to keratinocyte differentiation and barrier-related pathways, antioxidant / inflammation inhibition, and intercellular interactions, and the increase is greater than 150, with statistical significance, far higher than the control group. At the same time, the extracellular matrix metalloproteinase MMP3 is significantly reduced. The composition provided by this application can significantly increase the expression of human primary fibroblast growth factor and intercellular related genes, and the increase is greater than 150, with statistical significance, far higher than the comparative example group. Therefore, the composition provided by the present invention has multiple functions such as barrier repair, anti-oxidation, anti-inflammation, upregulation of cell-to-cell interactions such as integrin and growth factor to promote healing (scratch test) and repair.

[0058] The present application is further described below with reference to the following embodiments.

[0059] In the following examples, cyanobacteria were prepared according to the following steps:

[0060] (1) Using Phormidium uncinatum (accession number CCAP1462 / 7) as the raw material, a 250-20,000 ml pre-culture was performed by continuous seeding during the exponential growth phase of the cyanobacteria culture. The culture temperature was 25-26°C, white light was used for illumination, and a 130 L transparent plastic tube was used as a culture dish. After 10-20 days of culture, the cyanobacterial biomass was obtained;

[0061] (2) The cyanobacterial biomass was filtered through a 60 μm sieve, washed, and crushed several times under a pressure of 400 bar. The ground cyanobacterial biomass was filtered through a 0.2 μm membrane to obtain the supernatant;

[0062] (3) The supernatant is mixed with 5%-7% of maltodextrin, 1%-1.5% of magnesium lactate, and 0.5%-1% of calcium lactate based on the total weight of the supernatant, and homogenized to obtain a uniform solution. The solution is filtered through a 0.2 μm sterile filter, the pH is adjusted to 6.5, and the solution is dried to obtain cyanobacteria powder.

[0063] The tripeptide was palmitoyl tripeptide-5, a commercial product provided by READLINE Biotechnology Co., Ltd.

[0064] Bacillus fermentation products were commercially available and provided by Guangzhou Roundex Chemical Co., Ltd.

[0065] Iranian algae extract is a commercially available product provided by Lica Biotech Group;

[0066] The fermentation lysate of bifid yeast was a commercial product provided by Guangzhou Yanfu Biotechnology Co., Ltd.

[0067] Examples 1-2, Comparative Examples 1-5

[0068] The raw materials were mixed according to the formulation shown in Table 1 to obtain a composition.

[0069] Table 1 Formulas of compositions provided in Examples and Comparative Examples

[0070]

[0071] Test Example 1

[0072] 1. Cell Seeding

[0073] Fibroblasts were selected and, after cell recovery, seeded into 6-well plates and incubated overnight in a CO2 incubator (37°C, 5% CO2).

[0074] 2. Test Grouping

[0075] The experiment set up a blank control group BC, a negative control group NC, a positive control group PC, and a sample group, with three replicate wells in each group. The grouping is shown in Table 2. In Table 2, the test sample in each group was diluted with DMEM culture medium (Gibco) to a final concentration that met the dosage requirements in Table 2.

[0076] Table 2 Test grouping in Experiment 1

[0077]

[0078] 3.H2O2 stimulation

[0079] According to the test grouping, when the cell plating rate in the 6-well plate reached 40%-50%, all groups except the blank control group were stimulated with H2O2, and the induction time was 2 hours.

[0080] 4. Medication

[0081] After the induction time, the drugs were administered according to the test groups, with 3 replicates per group. 2 mL of culture medium was added to each well of the blank control group and the negative control group, 2 mL of culture medium containing quercetin was added to each well of the positive control group, and 2 mL of culture medium containing the corresponding concentration of the test sample was added to each well of the sample group. After the administration was completed, the 6-well plate was placed in a CO2 incubator (37°C, 5% CO2) and cultured for 24 hours. Repeat the above stimulation and administration steps 3 times.

[0082] 5. Immunofluorescence detection of type Ⅰ collagen (COLⅠ)

[0083] After incubation, the cells were washed and fixed with 4% paraformaldehyde for 30 minutes before immunofluorescence detection. The cells were photographed under a microscope and the images were collected and analyzed.

[0084] 6. Calculation of promotion rate

[0085] The type I collagen enhancement rate was calculated according to the following formula:

[0086] Improvement rate (%) = 100% × (sample group - negative control group) / negative control group;

[0087] 7. Statistical Analysis of Results

[0088] Comparisons between groups were performed using two-tailed t-tests. A p < 0.05 was considered significant.

[0089] The results are shown in Table 3 and Figure 1 , Table 3 is the statistical table of in vitro type I collagen test results, Figure 1 This is a statistical chart of the in vitro type I collagen test results.

[0090] Table 3 Statistics of in vitro type I collagen test results

[0091]

[0092] Note: The relative integrated optical density (IOD) value reflects the content of Collagen I. When statistically analyzed using the t-test method, significant differences compared with the BC group are indicated by #, p < 0.05 is indicated by #, and p < 0.01 is indicated by ##. Significant differences compared with the NC group are indicated by *, p < 0.05 is indicated by *, and p < 0.01 is indicated by **.

[0093] From Table 3 and Figure 1 It can be seen that:

[0094] Compared with the BC group, the NC group had a significantly decreased amount of type 1 collagen secretion, indicating that the stimulation conditions in this test were effective. Compared with the NC group, the PC group had a significantly increased amount of type 1 collagen secretion, indicating that the positive control in this test was effective.

[0095] According to the synergy calculation formula q=E A+B / (E A +E B -E A ×E B ) were calculated, and compared with Comparative Examples 1 and 2, Example 1 had a q of 1.25. Compared with Comparative Examples 5 and 2, Example 2 had a q of 1.14. This means that at this test concentration, the combination of the tripeptide, the Bacillus fermentation product, and the cyanobacteria produced a synergistic effect.

[0096] In addition, Comparative Example 3 used an extract of Pseudomonas aeruginosa in place of cyanobacteria, and Comparative Example 4 used a fermentation lysate of Bifida yeast in place of a fermentation product of Bacillus. A t-test (two-tailed statistical analysis) showed that the improvement rate of Comparative Example 3 compared with Example 1 was significantly different (p = 0.015, p < 0.05), and that of Comparative Example 4 compared with Example 1 was significantly different (p = 0.000, p < 0.01).

[0097] The above results mean that cyanobacteria, tripeptides and Bacillus fermentation products produced synergistic effects at the tested concentrations, and that the above effects could not be achieved by replacing the cyanobacteria or fermentation products in the above combination with the Iranian algae extract or the bifid yeast fermentation product lysate, respectively.

[0098] Test Example 2

[0099] The cell scratch test is widely used to evaluate the repair and cell migration promoting effects of raw materials in vitro.

[0100] 1. Cell culture: Immortalized keratinocytes HaCaT were used, the cells were revived, incubated in a CO2 incubator (37°C, 5% CO2), and the cells were plated for 24 hours.

[0101] 2. Experimental groups: The blank control NT was not treated, and the others were as shown in Table 4. In Table 4, the test samples of each group were prepared by diluting the corresponding composition with DMEM culture medium (Gibco) to make the final concentration meet the dosage concentration required in Table 4.

[0102] Table 4 Test groups for Experiment 2

[0103]

[0104] 3. Scratch treatment: scratch the cells and add the sample to be tested. Take photos and record them 0, 6, and 24 hours after adding the sample.

[0105] 4. Results see Figure 2 , Figure 2 The cell scratch test results provided for Experimental Example 2. Figure 2 It can be seen that at 24 hours, Example 3 can promote cell migration, which is better than Comparative Example 6, which means that cyanobacteria, tripeptides and Bacillus fermentation products have the effect of promoting cell migration and repairing skin under the test conditions.

[0106] Test Example 3

[0107] 1. Cell culture: Human keratinocytes (NHEKs) were seeded into 24-well plates and cultured in culture medium at 37°C and 5% CO2 for 24 hours.

[0108] 2. Test groups: The blank control NT was not treated, and the others were treated according to Table 5. In Table 5, the test samples of each group were prepared by diluting the corresponding composition with DMEM culture medium (Gibco) to make the final concentration meet the dosage concentration required in Table 5.

[0109] Table 5 Test groups for Experiment 3

[0110]

[0111] 3. Dosing: Replace the culture medium with the test sample as indicated in Table 5 and incubate for 24 hours. All experiments were performed with an n = 3 cells. At the end of the incubation period, harvest the cells and immediately freeze dry at 80°C.

[0112] 4. Differential expression analysis: TriPure Isolation Reagent was used to extract total RNA from cells collected under each experimental condition, converted to cDNA, and the expression of the target gene was detected by RT-qPCR.

[0113] The PCR (polymerase chain reaction) reaction mixture (final volume 10 μL) was prepared as follows:

[0114] 2.5 μL cDNA template;

[0115] 2 μL primers (forward and reverse), as shown in Table 6;

[0116] 5.5 μL reaction buffer;

[0117] Each experimental group and the untreated group had 3 replicate samples.

[0118] Table 6 Target gene primers

[0119]

[0120] 5. Data Processing: Raw data were analyzed using Microsoft Excel software. Relative gene expression was calculated by subtracting the GAPDH cycle value from the target gene PCR cycle value for each sample. The average of three samples in each group was calculated. The average cycle value of the untreated group was then subtracted from the experimental group's average cycle value to determine relative gene expression. A relative expression greater than 150% was considered statistically significant.

[0121] The results are shown in Tables 7 and 8. Table 7 shows the effects of the test samples on gene expression in human primary keratinocytes, and Table 8 shows the effects of the test samples on gene expression in human primary fibroblasts.

[0122] Table 7 Effects of test samples on gene expression in human primary keratinocytes

[0123]

[0124] Compared with Comparative Examples 7 and 8, Example 4 significantly upregulated the expression of genes involved in human primary keratinocyte differentiation and barrier-related pathways, antioxidant / anti-inflammatory pathways, and cell-cell interaction. The increase was statistically significant, exceeding 150%, and was significantly higher than that of the comparative example group. Furthermore, the expression of the extracellular matrix metalloproteinase MMP3 was significantly reduced. These results suggest that the composition provided by the present invention has the efficacy of barrier repair, antioxidant activity, promotion of cell-cell interaction, and inhibition of cell matrix degradation.

[0125] Table 8 Effects of test samples on gene expression in human primary fibroblasts

[0126]

[0127] Compared with Comparative Examples 7 and 8, Example 4 significantly upregulated the expression of human primary fibroblast growth factor and intercellular interaction genes, with an increase of greater than 150, showing a statistically significant difference and far exceeding that of the comparative example group. These results suggest that the composition provided by the present invention can promote healing (scratch test) and repair by upregulating intercellular interactions such as integrins and growth factors.

[0128] The above are only preferred embodiments of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. An algae-containing anti-aging repair composition, comprising cyanobacteria, a tripeptide, and a Bacillus fermentation product, wherein the mass ratio of the cyanobacteria, the tripeptide, and the Bacillus fermentation product is 0.01-0.5:0.1-1:0.2-2; The cyanobacteria is the cyanobacteria with a deposit number of CCAP 1462 / 7; The blue algae is prepared according to the following method: (1) Cultivating cyanobacteria under white light illumination; (2) crushing the cultured cyanobacterial biomass and filtering it to obtain a supernatant; (3) mixing the supernatant with α-hydroxy acid salt and maltodextrin, and sequentially homogenizing, filtering, and drying to obtain cyanobacteria powder; The tripeptide is palmitoyl tripeptide-5.

2. The anti-aging repair composition according to claim 1, characterized in that The mass ratio of the cyanobacteria, tripeptide and bacillus fermentation product is 0.01-0.25:0.1-0.6:0.2-1.

3. The anti-aging repair composition according to claim 2, characterized in that The mass ratio of the cyanobacteria, tripeptide and bacillus fermentation product is 0.05-0.25:0.6:1 or 0.01:0.13:0.

22.

4. The anti-aging repair composition according to claim 3, characterized in that The mass ratio of the cyanobacteria, tripeptide and bacillus fermentation product is 0.25:0.6:1 or 0.05:0.6:

1.

5. Use of the anti-aging and repairing composition according to any one of claims 1 to 4 in the preparation of anti-aging products or repair products.

6. Use of the anti-aging repair composition according to any one of claims 1 to 4 in the preparation of antioxidant and / or anti-inflammatory products.

7. The product is characterized in that include: The anti-aging repair composition according to any one of claims 1 to 4.

8. The product according to claim 7, characterized in that The product is a personal care product.

Images