Blood cell separation single sampling device

Abstract

The invention relates to the technical field of medical instruments, and discloses a blood cell separation single sampling device which comprises a first collecting bag and a second collecting bag, the first collecting bag is connected with a first input pipe and a first output pipe, the second collecting bag is connected with a second input pipe and a second output pipe, the first output pipe is communicated with the second input pipe, and the second output pipe is communicated with the second output pipe. The second output tube is connected with a leukocyte filter, the leukocyte filter is connected with a blood drawing tube, and the blood drawing tube and the second collecting bag are connected with a third input tube; control valves are arranged on the first output pipe, the second input pipe and the third input pipe; the control valve comprises a seat body, a vertical through hole is formed in the seat body, a sliding hole is further formed in the seat body, a sliding rod is arranged in the sliding hole in a sliding mode, a spring is connected between the sliding rod and the seat body, a pushing block is arranged on the sliding rod, an arc-shaped rotary valve plate is rotationally arranged on the outer side of the sliding rod, and the edge of the rotary valve plate abuts against the pushing block. The problem that a large number of erythrocytes are lost due to the fact that erythrocytes are easily mixed during leukocyte separation at present can be solved.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and particularly relates to a blood cell separation and apheresis device. Background Art

[0002] Leukemia originates from the malignant clonal proliferation of acquired early hematopoietic stem cells, generating a large number of leukemia cells that cannot differentiate and mature, accumulating in the bone marrow, increasing the bone marrow pressure, rupturing the bone marrow sinusoid barrier, and allowing a large number of immature leukemia cells to enter the blood circulation and infiltrate visceral tissues. During the interaction with vascular endothelial cells, soluble cytokines are released. Leukemia cells can also migrate around blood vessels, embolizing organs such as the brain, lungs, kidneys, and gastrointestinal tract, and can cause coagulopathy and tumor lysis syndrome, etc., thus increasing the early mortality rate of patients and the incidence and recurrence rate of extramedullary leukemia. Therefore, the American Society for Apheresis (ASFA) defines leukemia with a peripheral blood white blood cell (WBC) count > 100×10 / L as hyperleukocytic leukemia, and recommends leukostasis caused by abnormally elevated white blood cells in leukemia patients as a Class I indication for therapeutic leukapheresis. When the WBC count > 100×10%L, leukocyte reduction treatment should be urgently performed using a blood component separator to reduce the damage caused by leukemia cells to patients.

[0003] Leukapheresis is to send human peripheral blood into an apheresis instrument through an external connecting blood vessel, and the instrument centrifugally separates and collects to remove the white blood cells therein, while the remaining other blood components are returned to the human body through the circuit. However, when separating white blood cells, a certain amount of red blood cells are often mixed, resulting in a large loss of some red blood cells. Summary of the Invention

[0004] The present invention aims to provide a blood cell separation and apheresis device to solve the problem that when separating white blood cells currently, it is easy to mix red blood cells, resulting in a large loss of red blood cells.

[0005] To achieve the above object, the present invention adopts the following technical solution: A blood cell separation and apheresis device includes a first collection bag and a second collection bag. The first collection bag is connected with a first input tube and a first output tube, the second collection bag is connected with a second input tube and a second output tube, the first output tube is communicated with the second input tube, the second output tube is connected with a white blood cell filter, the white blood cell filter is connected with a blood vessel, and a third input tube is connected between the blood vessel and the second collection bag; control valves are provided on the first output tube, the second input tube, and the third input tube, and the position where the second input tube is connected to the second collection bag is above the position where the second output tube is connected to the second collection bag;

[0006] The control valve includes a seat body. A vertical through hole for the first output pipe, the second input pipe or the third input pipe to pass through is provided in the middle of the seat body. A sliding hole perpendicular to the through hole is also provided on the seat body. A sliding rod is slidably arranged in the sliding hole. One end of the sliding rod is located inside the through hole and the other end is located outside the seat body. A spring is connected between the sliding rod and the seat body. A pushing block is fixedly arranged on the sliding rod. A rotary valve plate is rotatably arranged on the outer side of the sliding rod. The rotary valve plate is arc-shaped, and the edge of the rotary valve plate abuts against the pushing block.

[0007] The principle and advantages of this solution are as follows: When blood cell separation and apheresis are required, after centrifuging venous blood to separate white blood cells, the white blood cells are introduced into the first collection bag. At this time, the white blood cells in the first collection bag still contain a relatively large amount of red blood cells and are a suspension of white blood cells and red blood cells. After this part of the suspension stands and layers in the first collection bag, the white blood cells and red blood cells located at the lower part of the suspension are introduced into the second collection bag through the second input pipe. After the suspension in the second collection bag stands and layers, it is introduced into a white blood cell filter for filtering of white blood cells. The filtered red blood cells then return to the human body through a blood drainage tube.

[0008] When filtering the suspension in the second collection bag, when the white blood cells are about to enter the second output pipe, part of the red blood cells are re-introduced into the second collection bag, causing the white blood cells to overflow and flow back to the first output pipe through the second input pipe, and finally discharged through the first output pipe. After all the white blood cells in the second collection bag are drained, the remaining red blood cells are filtered again by the white blood cell filter and then led back to the human body through the blood drainage tube, thereby separating and apherizing the buffy coat layer in multiple stages and effectively avoiding a large amount of loss of red blood cells during the white blood cell apheresis process.

[0009] The control valve is used to control the flow rates of the first output pipe, the second input pipe and the third input pipe. Since the rotary valve plate is arc-shaped, during the process of rotating the rotary valve plate, different parts of the rotary valve plate push the pushing block to move, causing the sliding rod to slide in the sliding hole. The end of the sliding rod squeezes the first output pipe, the second input pipe or the third input pipe to different degrees, so as to achieve the purpose of controlling the flow rate and ensure the efficiency and effect of the apheresis process. At the same time, since the rotary valve plate is arc-shaped, when the rotary valve plate rotates and moves away from the pushing block, the sliding rod loses the pushing force of the rotary valve plate and thus quickly automatically resets under the action of the spring to quickly squeeze the first output pipe, the second input pipe and the third input pipe, so as to quickly close the pipes when the separation of white blood cells and red blood cells is completed, thereby largely avoiding a large amount of loss of red blood cells.

[0010] Preferably, as an improvement, there are two groups of rotary valve plates, and the two groups of rotary valve plates are distributed in an S shape.

[0011] Through the above solution, when a group of rotary valve plates rotate past the thrust block, the slide rod quickly moves under the action of the spring, causing the thrust block to quickly abut against the other group of rotary valve plates. In this way, the next time it is necessary to open the first output pipe, the second input pipe, and the third input pipe, only by continuously rotating the rotary valve plate in the same direction can the other group of valve plates push the slide rod away from the first output pipe, the second input pipe, and the third input pipe. Thus, during adjustment, only a one-way operation on the rotary valve plate is required, making the operation simpler and more convenient.

[0012] Preferably, as an improvement, a ratchet wheel is fixedly provided on the rotary valve plate, a ratchet pawl is swingably provided on the outside of the ratchet wheel, the ratchet pawl is connected with a return spring, and the ratchet pawl abuts between the ratchet teeth of the ratchet wheel.

[0013] Through the above solution, the setting of the ratchet wheel and ratchet pawl makes the rotary valve plate rotatable only in one direction, which can prevent the rotary valve plate from rotating in the reverse direction and pushing the thrust block after passing over the thrust block, and can avoid the situation that the slide rod retracts after closing the first output pipe, the second input pipe, and the third input pipe and accidentally opens them.

[0014] Preferably, as an improvement, an extrusion plate is fixedly provided at one end of the slide rod close to the through hole, and a protrusion is provided on the side of the extrusion plate facing away from the slide rod.

[0015] Through the above solution, the setting of the extrusion plate can facilitate the extrusion of the slide rod on the first output pipe, the second input pipe, and the third input pipe, and improve the extrusion effect on the first output pipe, the second input pipe, and the third input pipe. The setting of the protrusion increases the closing effect when extruding the first output pipe, the second input pipe, and the third input pipe, and improves the separation effect of white blood cells and red blood cells.

[0016] Preferably, as an improvement, the extrusion plate is wavy, and an extrusion groove matching the shape of the extrusion plate is provided on the side of the through hole facing the extrusion plate.

[0017] Through the above solution, the extrusion plate is set to be wavy. When the extrusion plate extrudes the first output pipe, the second input pipe, and the third input pipe, it can cause the pipe to bend, which can quickly and effectively slow down the flow of liquid in the pipe, achieve the purpose of quickly closing the pipe, and improve the effect of leukapheresis.

[0018] Preferably, as an improvement, a lead screw is rotatably provided on the seat body, two sliding sleeves are sleeved on and threadedly connected to the lead screw, the sliding sleeves are slidably connected to the seat body, and clamping plates are fixedly provided on the sliding sleeves.

[0019] Through the above solution, during use, by rotating the lead screw, the two sliding sleeves can slide closer on the lead screw, thereby driving their respective clamping plates to move closer, so that the pipe can be clamped on the patient's clothing or external equipment to fix the pipe for apheresis treatment.

[0020] Preferably, as an improvement, a runner is fixedly provided at the end of the lead screw, and anti-slip lines are provided on the runner.

[0021] Through the above solution, the provided wheel disc and anti-slip lines can make the adjustment operation of the lead screw more convenient, and the practicability is better. Description of the Drawings

[0022] Figure 1 It is a schematic structural diagram of an embodiment of the present invention.

[0023] Figure 2 It is a structural diagram of a control valve, showing the state when the seat body is partially cut open.

[0024] Figure 3 It is Figure 2 an enlarged view of a partial area A in Detailed Embodiments

[0025] The following is a further detailed description through specific embodiments, but the embodiments of the present invention are not limited thereto. Unless otherwise specified, the technical means used in the following embodiments are conventional means well-known to those skilled in the art; the experimental methods used are all conventional methods; the materials, reagents, etc. used can all be obtained from commercial channels.

[0026] The reference numerals in the accompanying drawings of the specification include: the first collection bag 1, the second collection bag 2, the first input pipe 3, the first output pipe 4, the second input pipe 5, the second output pipe 6, the leukocyte filter 7, the blood drawing tube 8, the third input pipe 9, the control valve 10, the seat body 11, the through hole 12, the sliding hole 13, the sliding rod 14, the pushing block 15, the rotary valve plate 16, the ratchet wheel 17, the ratchet pawl 18, the lead screw 19, the sliding sleeve 20, the clamping plate 21, the runner 22, the pressing plate 23, and the pressing groove 24.

[0027] The embodiment is Figure 1 shown as follows: A blood cell separation and apheresis device includes a first collection bag 1 and a second collection bag 2. The first collection bag 1 is connected with a first input pipe 3 and a first output pipe 4. The second collection bag 2 is connected with a second input pipe 5 and a second output pipe 6. The first output pipe 4 is communicated with the second input pipe 5. The second output pipe 6 is connected with a leukocyte filter 7. The consumable for removing leukocytes in the leukocyte filter 7 adopts the separation adsorption and replacement treatment kit of Fresenius Kabi brand, and its model is P1YA White Blood Cell Set. The leukocyte filter 7 is connected with a blood drawing tube 8. A third input pipe 9 is connected between the blood drawing tube 8 and the second collection bag 2. Control valves 10 are provided on the first output pipe 4, the second input pipe 5, and the third input pipe 9, and the position where the second input pipe 5 is connected to the second collection bag 2 is above the position where the second output pipe 6 is connected to the second collection bag 2.

[0028] As Figure 2 andFigure 3 As shown in Figure 3 , the control valve 10 includes a seat body 11. A vertical through hole 12 is provided in the middle of the seat body 11 for the first output pipe 4, the second input pipe 5 or the third input pipe 9 to pass through. A sliding hole 13 perpendicular to the through hole 12 is also provided on the seat body 11. A sliding rod 14 is slidably arranged in the sliding hole 13. One end of the sliding rod 14 is located inside the through hole 12 and the other end is located outside the seat body 11. A spring is connected between the sliding rod 14 and the seat body 11. A pushing block 15 is fixedly arranged on the sliding rod 14. A rotary valve plate 16 is rotatably arranged on the outer side of the sliding rod 14. The rotary valve plate 16 is arc-shaped, and the edge of the rotary valve plate 16 abuts against the pushing block 15.

[0029] There are two groups of rotary valve plates 16, and the two groups of rotary valve plates 16 are distributed in an S shape.

[0030] A ratchet 17 is fixedly arranged on the rotary valve plate 16. A pawl 18 is swingably arranged on the outer side of the ratchet 17. The pawl 18 is connected with a return spring, and the pawl 18 abuts between the ratchet teeth of the ratchet 17.

[0031] One end of the sliding rod 14 close to the through hole 12 is fixedly provided with a pressing plate 23, and a protrusion is provided on the side of the pressing plate 23 facing away from the sliding rod 14.

[0032] The pressing plate 23 is wavy, and a pressing groove 24 matching the shape of the pressing plate 23 is provided on the side of the through hole 12 facing the pressing plate 23.

[0033] A lead screw 19 is rotatably arranged on the seat body 11. Two sliding sleeves 20 are sleeved and threadedly connected on the lead screw 19. The sliding sleeves 20 are slidably connected to the seat body 11, and clamping plates 21 are fixedly arranged on the sliding sleeves 20.

[0034] A runner 22 is fixedly arranged at the end of the lead screw 19, and anti-slip patterns are provided on the runner 22.

[0035] The specific implementation process is as follows:

[0036] When using this device for leukapheresis, after separating leukocytes from venous blood through an existing centrifugal device, the leukocytes are introduced into the first collection bag 1 through the first input pipe 3. At this time, the leukocytes in the first collection bag 1 still contain a relatively large amount of red blood cells, which is a suspension of leukocytes and red blood cells. After this part of the suspension is allowed to stand and layer in the first collection bag 1, the control valve 10 on the second input pipe 5 is opened, and the leukocytes and red blood cells located at the lower part of the suspension are introduced into the second collection bag 2 through the second input pipe 5. The remaining upper-layer liquid is discharged through the first output pipe 4 and enters the second collection bag 2. After the suspension in the second collection bag 2 is allowed to stand and layer, it is introduced into the leukocyte filter 7 for filtering of leukocytes. The filtered red blood cells return to the human body through the blood lead-in pipe 8. The leukocyte filter 7 in this embodiment is an existing technology, and the structure and principle will not be elaborated here. In actual application, a control valve 10 can also be provided on the second output pipe 6 to control the discharge of the liquid in the second collection bag 2.

[0037] When filtering the suspension in the second collection bag 2, when the white blood cells are about to enter the second output tube 6, the control valve 10 on the third input tube 9 is opened, and part of the red blood cells in the blood drawing tube 8 are re-input into the second collection bag 2 through the third input tube 9, so that the white blood cells in the second collection bag 2 overflow and flow back to the first output tube 4 through the second input tube 5, and are finally discharged through the first output tube 4. After the white blood cells in the second collection bag 2 are exhausted, the remaining red blood cells are filtered again by the white blood cell filter 7 and then drawn back into the human body by the blood drawing tube 8, so as to separate and apheresis the buffy coat in multiple stages, effectively avoiding a large amount of loss of red blood cells during the white blood cell apheresis process.

[0038] When adjusting the control valve 10 to control each input tube or output tube, combined with Figure 2 As shown, for example, rotate the rotary valve plate 16 in the counterclockwise direction, so that the rotary valve plate 16 pushes the abutting block 15 to move, thereby moving the slide rod 14 to the right. The left end of the slide rod 14 enters the slide hole 13. At this time, the input tube or output tube is opened, and the state is as Figure 2 presented. The input tube or output tube can input or output liquid at this time. When it is necessary to close the input tube or output tube, for example, when inputting red blood cells into the second collection bag 2 to make the white blood cells overflow and drain out from the second input tube 5, continue to rotate the rotary valve plate 16 in the counterclockwise direction at a small angle, so that the abutting block 15 disengages from the end of the rotary valve plate 16. At this time, the slide rod 14 instantaneously rebounds under the action of the spring, and promotes the slide rod 14 to quickly move leftward to reset. The pressing plate 23 presses against the inner wall of the through hole 12, thereby truncating the input tube or output tube, achieving the purpose of quickly closing the input tube or output tube. The pressing plate 23 cooperates with the pressing groove 24 to bend the input tube or output tube in a wavy shape, thereby effectively slowing down the flow of the liquid and enabling the liquid to quickly stop inputting or outputting.

[0039] When it is necessary to open the input tube or output tube again, rotate the rotary valve plate 16 counterclockwise to make Figure 2 the other set of rotary valve plates 16 on the left in

[0040] contact the abutting block 15 and push the abutting block to move, so that the slide rod 14 enters the slide hole 13 again.

[0041] The above are only embodiments of the present invention, and common general technical solutions and / or characteristics in the solution are not described in detail herein. It should be noted that for those skilled in the art, without departing from the technical solution of the present invention, several modifications and improvements can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the implementation effect of the present invention and the practicality of the patent. The protection scope required by this application shall be subject to the content of its claims, and the specific implementation manners and the like recorded in the specification can be used to interpret the content of the claims.

Claims

1. A blood cell separation and apheresis device, characterized in that: It comprises a first collecting bag and a second collecting bag, wherein the first collecting bag is connected to a first input tube and a first output tube, the second collecting bag is connected to a second input tube and a second output tube, the first output tube is communicated with the second input tube, the second output tube is connected to a leukocyte filter, the leukocyte filter is connected to a blood supply tube, and a third input tube is connected between the blood supply tube and the second collecting bag; control valves are arranged on the first output tube, the second input tube and the third input tube, and the part where the second input tube is connected to the second collecting bag is located above the part where the second output tube is connected to the second collecting bag; The control valve includes a seat body, a vertical through hole is provided in the middle of the seat body for the first output pipe, the second input pipe or the third input pipe to pass through, and a sliding hole perpendicular to the through hole is also provided on the seat body, a sliding rod is slidably provided in the sliding hole, one end of the sliding rod is located in the through hole, and the other end is located outside the seat body, a spring is connected between the sliding rod and the seat body, a push block is fixed on the sliding rod, and a rotating valve plate is rotatably provided on the outer side of the sliding rod, the rotating valve plate is arc-shaped, and the edge of the rotating valve plate is against the push block.

2. A blood cell separation and apheresis device according to claim 1, characterized in that: The rotary valve plates are provided with two groups, and the two groups of rotary valve plates are distributed in an S shape.

3. A blood cell separation and apheresis device according to claim 2, characterized in that: A ratchet is fixedly arranged on the rotary valve plate, a pawl is swingably arranged on the outer side of the ratchet, the pawl is connected with a return spring, and the pawl abuts between the ratchet teeth of the ratchet.

4. A blood cell separation and apheresis device according to claim 3, characterized in that: An extrusion plate is fixedly arranged at one end of the slide bar close to the through hole, and a protrusion is arranged on a side of the extrusion plate away from the slide bar.

5. A blood cell separation and apheresis device according to claim 4, characterized in that: The extrusion plate is wavy in shape, and an extrusion groove matching the shape of the extrusion plate is provided on one side of the through hole facing the extrusion plate.

6. A blood cell separation and apheresis device according to claim 5, characterized in that: A lead screw is rotatably arranged on the seat body, two sliding sleeves are sleeved and threadedly connected on the lead screw, the sliding sleeves are slidably connected to the seat body, and clamping plates are fixed on the sliding sleeves.

7. A blood cell separation and apheresis device according to claim 6, characterized in that: A rotating wheel is fixedly arranged at the end of the lead screw, and an anti-skid pattern is arranged on the rotating wheel.

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