Preparation method and application of plant grafting healing agent containing gleditsin

Abstract

The invention relates to the technical field of plant regulators, in particular to a preparation method and application of a plant grafting healing agent containing gleditsin. The plant growth regulator is prepared from the following raw materials in parts by weight: 0.005-8 parts of a plant growth regulator, 18-138 parts of a polymer film-forming agent, 0.5-10 parts of a tackifier, 0.8-28 parts of a bactericide, 5-62 parts of a nutritional agent and 5-21 parts of a water-retaining agent, the plant growth regulator comprises growth hormone and cytokinin, the mass ratio of the growth hormone to the cytokinin is (1-2): (2-5), and the bactericide is gleditsin. A brand-new plant grafting healing agent is prepared by utilizing gleditsin, so that the healing of a graft union can be well promoted, and the grafting survival rate is greatly improved.

Description

Technical Field

[0001] The present invention relates to the technical field of plant regulators, and more specifically, to a preparation method and application of a plant grafting healer containing gleditsin. Background Art

[0002] Although grafting technology is widely used in the seedling breeding of various plants, the survival rates of different tree species vary. In particular, the healing speed of the grafting interface determines whether the seedlings survive. In actual production, the grafting interface often fails due to infection. To ensure the survival rate of grafting, various chemical substances are used in grafting to promote the rapid healing of grafting wounds. However, they all have their limitations.

[0003] Gleditsia sinensis Lam., also known as Chinese honey locust, belongs to the Leguminosae family and the Gleditsia genus. China is rich in Gleditsia sinensis resources, which are mainly distributed in the Northeast, North China, East China, South China, as well as Sichuan, Guizhou and other places. The pods of Gleditsia sinensis contain more than 30% saponins, which is the woody plant resource with the highest saponin content found so far. In production, the saponins extracted from the pods of Gleditsia sinensis are often called gleditsin (extracted according to the industry standard "LY / T 3151-2019"). Gleditsin is a pentacyclic triterpenoid natural surfactant, which is easily biodegradable, non-toxic and harmless to the human body, has good surface activity, and has anti-inflammatory, antibacterial, dehumidifying and insecticidal effects. The inventor found during the Gleditsia sinensis grafting experiment that gleditsin not only has a strong antibacterial effect, but also is a good surfactant, is more easily biodegradable, and is non-toxic and harmless to the human body and the environment.

[0004] "Preparation and Use Method of a Plant Grafting Healer" with the application number 201210096796.6. This invention consists of Agent A and Agent B. Agent A is composed of Kathon (isothiazolinone), naphthalene acetic acid, and distilled water. Agent B is a superabsorbent resin with a water absorption multiple of 400 - 500 times and a mesh number of 100 - 120. When in use, Agent A and Agent B are combined and applied around the grafting interface. Although this invention reduces costs and is easy to operate, isothiazolinone is a potentially acutely toxic chemical substance, corrosive, irritating to the skin and eyes, toxic to fish, and cannot be poured into lakes, rivers, ponds, or public waters. There is even a view that it is a neurotoxin with cytotoxicity and neurotoxicity. It is treated as a dangerous good in production. If the operation is improper during grafting, it will cause harm to the human body and the environment. In addition, there are many types of superabsorbent resins, generally divided into two categories. One is acrylamide - acrylate copolymer cross - linked substances (such as polyacrylamide, sodium polyacrylate, potassium polyacrylate, ammonium polyacrylate, etc.); the other is starch - grafted acrylate copolymer cross - linked substances (starch - grafted acrylate). This invention does not specifically state which one is used. If acrylamide - acrylate copolymer cross - linked substances are used, their water absorption performance is relatively poor and they are toxic substances, easily endangering the human body and the environment. Summary of the Invention

[0005] The present invention provides a preparation method and application of a plant grafting healer containing saponin. A brand - new plant grafting healer is prepared by using saponin, which can well promote the healing of the grafting interface and greatly improve the grafting survival rate.

[0006] In the first aspect, the present invention provides a plant grafting healer containing saponin, which is prepared from raw materials with the following weight components: 0.005 - 8 parts of a plant growth regulator, 18 - 138 parts of a high - molecular film - forming agent, 0.5 - 10 parts of a tackifier, 0.8 - 28 parts of a bactericide, 5 - 62 parts of a nutrient agent, 5 - 21 parts of a water - retaining agent. The plant growth regulator includes a growth hormone and a cytokinin, and the mass ratio of the growth hormone to the cytokinin is 1 - 2:2 - 5. The bactericide is saponin.

[0007] Preferably, the high - molecular film - forming agent is polyvinyl alcohol.

[0008] Preferably, the water - retaining agent is a starch - grafted acrylic acid synthetic cross - linked superabsorbent resin, which is prepared by reacting starch with vinyl monomers under the action of an initiator or through radiation initiation.

[0009] Preferably, the growth hormone is one or more of indoleacetic acid and α - naphthalene acetic acid.

[0010] Preferably, the cytokinin is one or more of isopentenyl adenine, abscisic acid, and jasmonic acid substances.

[0011] Preferably, the tackifier is one or more of hydroxypropyl cellulose, hydroxyethyl cellulose, carboxymethyl cellulose, ethylene glycol, and β-1,4-glucanase.

[0012] In a second aspect, the present invention provides a method for preparing a plant grafting healing agent containing saponin, comprising the following steps:

[0013] (1) Dissolve alcohol and growth hormone to obtain a mixed solution;

[0014] (2) Put the mixed solution, distilled water, cytokinin, high molecular film-forming agent, tackifier, bactericide, nutrient, and water retention agent into a beaker, stir evenly, and let stand for 15 - 25 min to obtain the plant grafting healing agent.

[0015] In a third aspect, the present invention provides an application of a plant grafting healing agent containing saponin, and the plant grafting healing agent containing saponin is applied to plant wound healing.

[0016] Preferably,

[0017] In summary, the present invention has the following beneficial effects:

[0018] 1. The saponin in the present invention is a natural bactericide, which has a particularly good inhibitory and eliminating effect on a variety of sensitive bacteria and fungi, and has good surface activity, which can avoid the infection of the grafting interface and promote the fitting of the grafting interface.

[0019] 2. The starch-grafted acrylic acid synthetic cross-linked superabsorbent resin in the present invention: refers to a water-absorbing starch-grafted copolymer resin prepared by reacting starch with vinyl monomers under the action of an initiator or through radiation initiation. Compared with other superabsorbent resins such as polyacrylamide and sodium polyacrylate, the starch-grafted acrylic acid synthetic cross-linked superabsorbent resin has the advantages of reusability, biodegradability, high hydrophilicity of hydroxyl groups in glucose units, deformable structures of a large number of highly chemically reactive functional group polymers, relatively simple synthesis process, and low product cost, and can be used as a water retention agent for seedling grafting.

[0020] 3. Polyvinyl alcohol in the present invention is a very safe polymer organic substance, which is non-toxic and has no side effects on both humans and plants, has good biocompatibility, is a commonly used safe adhesive and film-forming agent, can make the grafting interface fit better, and forms a film at the grafting interface to better protect the grafting interface.

[0021] 4. Gibberellin in the present invention is a broad-spectrum plant hormone, which can promote the healing of the grafting interface. Indoleacetic acid is a broad-spectrum hormone reagent that stimulates plant growth and can promote the healing of the grafting interface.

[0022] 5. The α-naphthylacetic acid in the present invention is a broad-spectrum plant growth regulator that promotes cell division and growth metabolism, can promote the activity of the cambium, thereby promoting wound healing and improving the survival rate of grafting, and can be transported to the action site along with the nutrient flow to promote the growth of grafted seedlings. 75% alcohol is used to dissolve α-naphthylacetic acid.

[0023] 6. The plant grafting healing agent containing saponin prepared in the present invention is used for sterilizing the plant grafting interface, the plant growth regulator, and the water to be in place simultaneously, and has functions such as disinfection, sterilization, water retention, water absorption, and preventing infection, thereby promoting the rapid healing of the grafting interface, improving the survival rate of grafting, and the reagents used are all harmless to the human body and the plant body, and are easily biodegradable and will not cause pollution to the environment.

[0024] 7. The plant grafting healing agent containing saponin prepared in the present invention is flexible and convenient to use in plants. The product has low production cost, good effect, and simple application, and can be widely applied to the grafting of most plants such as Gleditsia sinensis, Camellia oleifera, Sapindus mukorossi, and Idesia polycarpa.

[0025] It should be understood that the above general description and the following detailed description are only exemplary and explanatory, and cannot limit the protection scope of the present invention. Detailed implementation mode

[0026] The present invention will be further described in detail below in conjunction with the embodiments. It should be specially noted that: for those not specifying specific conditions in the following embodiments, they are carried out according to conventional conditions or the conditions recommended by the manufacturer. Except for special instructions, the raw materials used in the following embodiments can all be obtained from ordinary commercial sources.

[0027] Embodiment

[0028] Embodiment 1

[0029] A plant grafting healing agent containing saponin is prepared from raw materials with the following weight components: 0.5 part of plant growth regulator, 18 parts of polymer film-forming agent, 0.5 part of tackifier, 0.8 part of bactericide, 5 parts of nutrient agent, 5 parts of water-retaining agent. The plant growth regulator includes growth hormone and cytokinin, and the mass ratio of the growth hormone to the cytokinin is 1:2. The bactericide is saponin; the polymer film-forming agent is polyvinyl alcohol; the water-retaining agent is starch-grafted acrylic acid synthetic cross-linked superabsorbent resin, and the starch-grafted acrylic acid synthetic cross-linked superabsorbent resin is prepared by reacting starch with vinyl monomer under the action of an initiator or through radiation initiation; the growth hormone is a mixture of indoleacetic acid and α-naphthylacetic acid in a mass ratio of 1:2; the cytokinin is isopentenyladenine; the tackifier is hydroxypropyl cellulose.

[0030] A preparation method of a plant grafting healing agent containing saponin includes the following steps:

[0031] (1) Dissolve alcohol and growth hormone to obtain a mixed solution;

[0032] (2) Put the mixed solution, distilled water, cytokinin, polymer film-forming agent, thickener, bactericide, nutrient agent and water retention agent into a beaker, stir evenly, and let stand for 15 minutes to obtain a plant grafting healing agent.

[0033] Example 2

[0034] A plant grafting healing agent containing saponin is prepared from raw materials with the following weight components: 1.2 parts of plant growth regulator, 25 parts of polymer film-forming agent, 2.5 parts of thickener, 2.3 parts of bactericide, 15 parts of nutrient agent, 8 parts of water retention agent. The plant growth regulator includes growth hormone and cytokinin, and the mass ratio of growth hormone to cytokinin is 1:3. The bactericide is saponin; the polymer film-forming agent is polyvinyl alcohol; the water retention agent is starch-grafted acrylic acid synthetic cross-linked superabsorbent resin, which is prepared by reacting starch with vinyl monomer under the action of an initiator or through radiation initiation; the growth hormone is indoleacetic acid and α-naphthylacetic acid mixed in a mass ratio of 2:1; the cytokinin is isopentenyl adenine; the thickener is hydroxypropyl cellulose.

[0035] A preparation method of a plant grafting healing agent containing saponin includes the following steps:

[0036] (1) Dissolve alcohol and growth hormone to obtain a mixed solution;

[0037] (2) Put the mixed solution, distilled water, cytokinin, polymer film-forming agent, thickener, bactericide, nutrient agent and water retention agent into a beaker, stir evenly, and let stand for 15 minutes to obtain a plant grafting healing agent.

[0038] Example 3

[0039] A plant grafting healing agent containing saponin is prepared from raw materials with the following weight components: 5.1 parts of plant growth regulator, 48 parts of polymer film-forming agent, 6.3 parts of thickener, 12.5 parts of bactericide, 31 parts of nutrient agent, 16 parts of water retention agent. The plant growth regulator includes growth hormone and cytokinin, and the mass ratio of growth hormone to cytokinin is 1:4. The bactericide is saponin; the polymer film-forming agent is polyvinyl alcohol; the water retention agent is starch-grafted acrylic acid synthetic cross-linked superabsorbent resin, which is prepared by reacting starch with vinyl monomer under the action of an initiator or through radiation initiation; the growth hormone is indoleacetic acid and α-naphthylacetic acid mixed in a mass ratio of 1:2; the cytokinin is isopentenyl adenine; the thickener is hydroxypropyl cellulose.

[0040] A preparation method of a plant grafting healing agent containing saponin includes the following steps:

[0041] (1) Dissolve alcohol and growth hormone to obtain a mixed solution;

[0042] (2) Put the mixed solution, distilled water, cytokinin, polymer film-forming agent, thickening agent, bactericide, nutrient agent and water-retaining agent into a beaker, stir evenly, and obtain a plant grafting healing agent after standing for 20 min.

[0043] Example 4

[0044] A plant grafting healing agent containing saponin is prepared from raw materials with the following weight components: 8 parts of plant growth regulator, 138 parts of polymer film-forming agent, 10 parts of thickening agent, 28 parts of bactericide, 62 parts of nutrient agent, 21 parts of water-retaining agent. The plant growth regulator includes growth hormone and cytokinin, and the mass ratio of growth hormone to cytokinin is 1:5. The bactericide is saponin; the polymer film-forming agent is polyvinyl alcohol; the water-retaining agent is starch-grafted acrylic acid synthetic cross-linked superabsorbent resin, which is prepared by reacting starch with vinyl monomer under the action of an initiator or through radiation initiation; the growth hormone is indoleacetic acid and α-naphthylacetic acid mixed in a mass ratio of 1:3; the cytokinin is isopentenyladenine; the thickening agent is hydroxypropyl cellulose.

[0045] A preparation method of a plant grafting healing agent containing saponin includes the following steps:

[0046] (1) Dissolve alcohol and growth hormone to obtain a mixed solution;

[0047] (2) Put the mixed solution, distilled water, cytokinin, polymer film-forming agent, thickening agent, bactericide, nutrient agent and water-retaining agent into a beaker, stir evenly, and obtain a plant grafting healing agent after standing for 25 min.

[0048] Comparative Example 1

[0049] Replace the healing agent with the commercially available Sichuan Guoguang Hutu Smearing Agent.

[0050] Comparative Example 2

[0051] Replace the healing agent with 1.8% Xinjunan acetate from Xi'an Jiake Agrochemical Co., Ltd.

[0052] Comparative Example 3

[0053] Replace the healing agent with the commercially available methylthionaphthylacetic acid.

[0054] Table 1 shows the treatment effects of different healing agents in Examples 1-4 and Comparative Examples 1-3 on the wounds of Camellia oleifera

[0055]

[0056]

[0057] Table 2 shows the treatment effects of different wound healing agents on the wounds of Sapindus mukorossi in Examples 1-4 and Comparative Examples 1-3.

[0058]

[0059] Table 3 shows the treatment effects of different wound healing agents on the wounds of Idesia polycarpa in Examples 1-4 and Comparative Examples 1-3.

[0060]

[0061] Table 4 shows the treatment effects of different wound healing agents on the wounds of Gleditsia sinensis in Examples 1-4 and Comparative Examples 1-3.

[0062]

[0063]

[0064] As can be seen from Tables 1-4, the plant grafting wound healing agent containing gleditsin of the present invention is applied to grafting of most plants such as Gleditsia sinensis, Camellia oleifera, Sapindus mukorossi, and Idesia polycarpa, and has good healing effects on trunk wounds.

[0065] As described above, the above are only exemplary specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto. Any changes or substitutions that can be easily thought of by those skilled in the art within the technical scope disclosed by the present invention should be covered within the protection scope of the present invention. Therefore, the protection scope of the present invention should be subject to the protection scope of the claims.

Claims

1. A plant grafting healing agent containing saponin, characterized in that: The invention is prepared from the following raw materials with weight components: 0.005-8 parts of plant growth regulator, 18-138 parts of polymer film-forming agent, 0.5-10 parts of viscosity enhancer, 0.8-28 parts of fungicide, 5-62 parts of nutrient agent and 5-21 parts of water retaining agent. The plant growth regulator comprises growth hormone and cytokinin, the mass ratio of growth hormone to cytokinin is 1-2:2-5, and the fungicide is saponin.

2. The plant grafting healing agent containing saponin according to claim 1, characterized in that: The polymer film-forming agent is polyvinyl alcohol.

3. The plant grafting healing agent containing saponin according to claim 1, characterized in that: The water-retaining agent is a cross-linked super absorbent resin synthesized by starch grafting acrylic acid. The cross-linked super absorbent resin synthesized by starch grafting acrylic acid is prepared by reacting starch and vinyl monomer under the action of an initiator or through radiation initiation.

4. The plant grafting healing agent containing saponin according to claim 1, characterized in that: Growth hormone is one or more of indoleacetic acid and α-naphthylacetic acid.

5. The plant grafting healing agent containing saponin according to claim 1, characterized in that: The cytokinin is one or more of isopentenyl adenine, abscisic acid and jasmonic acid.

6. The plant grafting healing agent containing saponin according to claim 1, characterized in that: The viscosity increasing agent is one or more of hydroxypropyl cellulose, hydroxyethyl cellulose, methyl cellulose, ethylene glycol and β-1,4-glucanase.

7. The plant grafting healing agent containing saponin according to claim 1, characterized in that: The nutrient agent is one or both of gibberellin and vitamin C.

8. The method for preparing the plant grafting healing agent containing saponin according to any one of claims 1 to 7, characterized in that: The following steps are involved: (1) dissolving alcohol and growth hormone to prepare a mixed solution; (2) The mixed solution, distilled water, cytokinin, polymer film-forming agent, viscosity enhancer, fungicide, nutrient and water-retaining agent are placed in a beaker and stirred evenly. The mixture is allowed to stand for 15-25 minutes to obtain a plant grafting healing agent.

9. The use of the plant grafting healing agent containing saponin according to claim 1, characterized in that: The plant grafting healing agent containing saponin is used in plant wound healing.

10. The use of the plant grafting healing agent containing saponin according to claim 9, characterized in that: The specific method of applying the plant grafting healing agent containing saponin is: when the plant scion is grafted onto the rootstock, the plant grafting healing agent containing saponin is applied around the grafting site, and then the grafting site is bound with a plastic film strip.

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