Medicament composition for improving thousand grain weight of wheat

Abstract

The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition for increasing the thousand grain weight of wheat. The bactericidal composition is characterized in that effective components of the bactericidal composition are formed by compounding murraya paniculata ketone and tebuconazole, and the mass ratio of murraya paniculata ketone to tebuconazole is (1-40): (15-1). When the murrayone in the bactericidal composition is compounded with other effective components, the bactericidal composition has a synergistic effect on wheat powdery mildew pathogenic bacteria within a certain mass ratio range, the prevention and treatment effect on wheat powdery mildew can be improved, the influence of wheat powdery mildew on wheat thousand seed weight is reduced, and further the thousand seed weight of wheat can be increased. And secondly, when the murrayone in the bactericidal composition is compounded with other effective components, the development of drug resistance of pathogenic bacteria can be effectively delayed, so that the drug resistance risk caused by application of a single effective component can be reduced. In addition, the bactericidal composition can reduce the application amount of the effective components, reduce the prevention and treatment cost, and provide support for development of novel medicaments.

Description

Technical Field

[0001] The present invention belongs to the technical field of pesticides, and specifically relates to a medicament composition for increasing the thousand-grain weight of wheat. Background Art

[0002] Powdery mildew of wheat is a worldwide disease caused by Blumeria graminis f. sp. Tritici, which is a facultative parasite infecting wheat. At the initial stage of the disease, yellowish dots can be seen on the diseased part. As the disease progresses, the dots gradually develop into lesions, with a powdery mildew layer on the surface. When the disease is less severe, the mildew spots are scattered; as the disease worsens, the mildew spots gradually expand into patches, eventually covering the entire leaf; the tissue below and around the lesion turns chlorotic, the diseased leaves turn yellow and wither prematurely. If the stem and leaf sheath are affected, it will cause the whole plant to lodge, the plant is short and weak, the spike is small and has few grains, and the thousand-grain weight decreases significantly, ultimately affecting the wheat yield.

[0003] In agricultural production, planting disease-resistant varieties is the most economical means to control powdery mildew of wheat. However, due to the fast reproduction and mutation rate of the powdery mildew pathogen of wheat, the disease-resistant varieties lose their disease resistance after being widely planted for several years. At present, the most important measure to control powdery mildew of wheat is still chemical control. Commonly used chemical agents include triazole agents such as triadimefon. However, due to the long-term unreasonable use of chemical agents with a single active ingredient, powdery mildew has developed varying degrees of resistance to many existing chemical agents. In order to improve the control effect on powdery mildew, reduce the impact of powdery mildew on the thousand-grain weight of wheat, and at the same time slow down the development rate of pathogen resistance, it is necessary to develop new agents.

[0004] Murrangatin, CAS No.: 19668-69-0, molecular formula: C 15 H 14 O4, derived from plants of the Rutaceae family. Its molecular structural formula is as follows:

[0005]

[0006] CN201510815853.5 discloses the use of a natural compound murrangatin as an agricultural fungicide. In its specification, it is disclosed that murrangatin has control effects on various diseases such as Sclerotinia sclerotiorum of rape, sheath blight of rice, gray mold of strawberry, head blight of wheat, anthracnose of pepper, and early blight of tomato.

[0007] Combining the active ingredients of different pesticides is a convenient way to develop new agents. The inventor found through indoor bioactivity tests that after murrangatin is compounded with metconazole, tebuconazole or prothioconazole, it has a synergistic effect on controlling powdery mildew of wheat within a certain mass ratio range. At present, there are no relevant reports on the compounding of murrangatin with metconazole, tebuconazole or prothioconazole.

[0008] The information disclosed in this background section is only intended to enhance the overall understanding of the present invention and should not be regarded as an admission or any form of implication that this information constitutes prior art already known to those of ordinary skill in the art. Summary of the Invention

[0009] An object of the present invention is to provide a pharmaceutical composition for increasing the thousand-grain weight of wheat. Compared with a single active ingredient, it can improve the control effect on wheat powdery mildew, thereby being beneficial to reducing the impact of powdery mildew on the thousand-grain weight of wheat. At the same time, it can also delay the development of pathogen resistance, and thus can provide support for the development of new pharmaceuticals.

[0010] To achieve the above object, the present invention provides the following technical solutions:

[0011] A first object of the present invention is to provide a bactericidal composition, wherein the active ingredients of the bactericidal composition are compounded by murrangatin and metconazole, tebuconazole or prothioconazole.

[0012] More preferably, the mass ratio of murrangatin to metconazole is 1 - 5:20 - 1.

[0013] More preferably, the mass ratio of murrangatin to tebuconazole is 1 - 40:15 - 1.

[0014] More preferably, the mass ratio of murrangatin to prothioconazole is 1 - 20:8 - 1.

[0015] A second object of the present invention is to provide a fungicide, which comprises the above-mentioned bactericidal composition and other auxiliary components.

[0016] A third object of the present invention is to provide the application of the above-mentioned bactericidal composition in the control of wheat fungal diseases.

[0017] A fourth object of the present invention is to provide the application of the above-mentioned bactericidal composition in the control of wheat powdery mildew.

[0018] A fifth object of the present invention is to provide the application of the above-mentioned bactericidal composition in increasing the thousand-grain weight of wheat.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] (1) When murrangatin in the bactericidal combination of the present invention is compounded with other active ingredients, it has a synergistic effect on the pathogen of wheat powdery mildew within a certain mass ratio range, can improve the control effect on wheat powdery mildew, reduce the impact of wheat powdery mildew on the thousand-grain weight of wheat, and thus helps to increase the thousand-grain weight of wheat.

[0021] (2) When murrangatin is compounded with other active ingredients in the bactericidal composition of the present invention, the development of pathogen drug resistance can be effectively delayed, thereby reducing the drug resistance risk brought about when applying a single active ingredient.

[0022] (3) The bactericidal composition of the present invention can reduce the application amount of the active ingredient, reduce the control cost, and provide support for the development of new drugs. Detailed implementation manners

[0023] The present invention can be better understood according to the following examples. However, those skilled in the art can easily understand that the content described in the examples is only used to illustrate the present invention and should not and will not limit the present invention described in detail in the claims.

[0024] Embodiment : Indoor bioactivity test after compounding murrangatin

[0025] 1. Test crops: Wheat seeds of "Zhengmai 9023", potted and cultured until the 2-3 true leaf stage, 10 plants per pot, numbered for standby.

[0026] 2. Test agents

[0027] 98% murrangatin technical (Shanghai Yuanye Bio-Technology Co., Ltd.)

[0028] 95% metconazole technical (Shanghai Heteng Fine Chemical Co., Ltd.)

[0029] 97% tebuconazole technical (Shenyang Kechuang Chemical Co., Ltd.)

[0030] 95% prothioconazole technical (Ningxia Yifan Biotechnology Co., Ltd.)

[0031] 3. Test method (refer to NY / T 1156.4-2008 "Pesticide indoor bioassay test guidelines Fungicides Part 4: Test potted method for controlling wheat powdery mildew")

[0032] 3.1 Prepare agents: Dissolve the technical in dimethyl sulfoxide and dilute with 0.1% Tween-80 aqueous solution to prepare single-agent mother liquors respectively. Set multiple groups of ratios, and set 5 series of mass concentration gradients for each single agent and the mixture of each group of ratios;

[0033] 3.2 Inoculation treatment: Evenly shake off the fresh spores of Blumeria graminis f. sp. Tritici produced on the diseased wheat leaves within 24 hours and inoculate them on the test wheat seedlings;

[0034] 3.3 Chemical agent treatment: After 24 h, the liquid medicine was evenly sprayed on the leaves of the tested wheat seedlings until they were all wet. There were 3 pots for each treatment, and a treatment with only 0.1% Tween-80 aqueous solution was set as the blank control. After natural air drying, they were moved to a constant temperature room and continued to be cultured under the conditions of a temperature of 22 °C and a light:dark ratio of 16 h:8 h.

[0035] 3.4 Investigation and calculation: When the disease leaf rate of the blank control reached more than 80%, the disease incidence of each treatment was investigated according to the grading standard in Table 1 below. According to the investigation data, the disease index and control effect of each treatment were calculated. Among them, the grading standard is shown in Table 1.

[0036] Table 1 Disease grade assessment standard

[0037]

[0038] 3.5 Data analysis: DPS software was used for data statistical analysis. The logarithm value of the fungicide concentration was used as x, and the corresponding probit value of the control effect was used as y for linear regression to obtain the virulence regression equation and the virulence EC 50 value of the agent against the target pathogen, and the co-toxicity coefficient (CTC) was calculated according to the Sun Yunpei method.

[0039] 3.6 Efficacy evaluation: According to the calculated co-toxicity coefficient, the synergistic effect of the agent was evaluated. CTC ≤ 80 was antagonistic effect, 80 < CTC < 120 was additive effect, and CTC ≥ 120 was synergistic effect. The results are shown in Tables 2 - 4.

[0040] Table 2 Indoor bioactivity test results of the combination of murrangatin and metconazole against wheat powdery mildew

[0041]

[0042] As can be seen from Table 2, within the mass ratio of 1 - 5:20 - 1, the co-toxicity coefficient of the combination of murrangatin and metconazole against wheat powdery mildew was greater than 120, showing a synergistic effect.

[0043] Table 3 Indoor bioactivity test results of the combination of murrangatin and tebuconazole against wheat powdery mildew

[0044]

[0045] As can be seen from Table 3, within the mass ratio of 1 - 40:15 - 1, the co-toxicity coefficient of the combination of murrangatin and tebuconazole against wheat powdery mildew was greater than 120, showing a synergistic effect.

[0046] Table 4 Indoor bioactivity test results of the combination of murrangatin and prothioconazole against wheat powdery mildew

[0047]

[0048] As can be seen from Table 4, within the mass ratio range of 1 - 20:8 - 1, the co-toxicity coefficient of murrangatin and prothioconazole against Blumeria graminis f. sp. tritici is greater than 120, showing a synergistic effect.

[0049] In summary, when murrangatin in the bactericidal combination of the present invention is compounded with metconazole, tebuconazole or prothioconazole, it has a synergistic effect on the pathogen of Blumeria graminis f. sp. tritici within a certain mass ratio range, can improve the control effect on wheat powdery mildew, reduce the impact of wheat powdery mildew on the thousand-grain weight of wheat, and thus helps to increase the thousand-grain weight of wheat.

[0050] The foregoing description of specific exemplary embodiments of the invention has been presented for purposes of illustration and example. These descriptions are not intended to limit the invention to the precise forms disclosed, and it is apparent that many modifications and variations are possible in light of the above teaching. The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the invention and its practical application, so that those skilled in the art can implement and utilize the various different exemplary embodiments of the invention, as well as various different selections and modifications. The scope of the invention is intended to be defined by the claims and their equivalents.

Claims

1. A bactericidal composition, characterized in that, The active ingredient of the bactericidal composition is composed of murrangatin and tebuconazole, and the mass ratio of murrangatin to tebuconazole is 1-40:15-1.

2. A fungicide, characterized in that, The bactericide comprises the bactericidal composition described in claim 1 and other auxiliary components.

3. Application of the bactericidal composition described in claim 1 in preventing and controlling powdery mildew of wheat.

4. Application of the bactericidal composition described in claim 1 in increasing the thousand-grain weight of wheat.

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