Application of 5-azacytosine as plant growth regulator and regulation and control method
By spraying 5-azacytosine nucleoside solution, the phototropic growth of cucumber seedlings was inhibited, and the problem of irregular plant types during seedling cultivation was solved, the seedling efficiency and plant uniformity were improved, and management costs were reduced.
Patent Information
- Application Number
- CN202510347861.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-24
- Publication Date
- 2025-06-27
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
During the seedling cultivation process, the plant shape is uneven due to photon growth, which affects the uniformity and quality of the plant, increases management costs and limits the plant density of the seedling bed.
5-azacytosine nucleoside is used as a plant growth regulator. The solution is sprayed to inhibit the phototropic growth of cucumber seedlings, help the seedlings adapt to irregular light conditions, and promote the seedlings to grow upright and evenly.
It effectively inhibits the bending degree of cucumber seedlings toward light, improves the seedling cultivation efficiency, reduces the cost of manual adjustment, and promotes the neatness and uniformity of cucumber plant types.
Smart Images
Figure CN120203055A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of vegetable seedling raising, and particularly to the application of 5-azacytidine as a plant growth regulator and a regulation method. Background Art
[0002] The demand for cucumber (Cucumis Sativus L.) has been continuously increasing globally due to its refreshing taste and rich nutrition. To meet this demand, it is crucial to improve the cucumber seedling raising efficiency and achieve industrialization. The uneven plant type of cucumber seedlings during the seedling raising process is mainly due to the external environment of uneven light source distribution in the greenhouse or seedling raising room. In these controlled environments, the light usually comes from a single direction or a specific angle, prompting the seedlings to grow towards the light source to optimize photosynthesis. However, this phototropism will cause the seedlings to grow bent during the seedling raising stage, affecting the uniformity and quality of the plants, increasing the management cost, and limiting the planting density of the seedbed. When regulating the plant type of cucumber, it is possible to inhibit the phototropism of cucumber seedlings, prompt the seedlings to grow upright and uniformly, thereby improving the seedling raising efficiency, reducing the management cost, and promoting the industrial development of cucumber seedling raising.
[0003] However, the common methods for regulating the cucumber plant type at present include: setting multi-angle light sources in the seedling raising environment to provide uniform light, or manually adjusting the positions of the seedlings regularly to ensure uniform growth of the plants. However, these methods are time-consuming and laborious, not only increasing the labor intensity during the seedling raising process, but also increasing the production cost and limiting the efficiency of large-scale seedling raising.
[0004] Therefore, finding a more efficient and labor-saving plant type regulation method is of great significance for optimizing the cucumber seedling raising process. Summary of the Invention
[0005] The purpose of the present invention is to provide the application of 5-azacytidine as a plant growth regulator and a regulation method. The 5-azacytidine can inhibit the phototropic growth of plants, help the seedlings adapt to irregular light conditions, prompt the seedlings to grow upright and uniformly, and reduce the manual adjustment cost, thereby solving the problem of uneven plant type caused by phototropism during the seedling raising process.
[0006] In order to achieve the above invention purpose, the present invention provides the following technical solutions:
[0007] The present invention provides the application of 5-azacytidine as a plant growth regulator.
[0008] Preferably, the effective concentration of the 5-azacytidine is 150 - 250 μM.
[0009] Preferably, the plant regulated by the 5-azacytidine as a plant growth regulator is cucumber.
[0010] The present invention also provides the use of 5-azacytidine in the preparation of a product having the function of regulating plant architecture.
[0011] Preferably, the plant is cucumber; the dosage forms of the product include solution, spray and granule.
[0012] The present invention also provides a method for regulating plant architecture, comprising the following steps:
[0013] (1) Prepare a 5-azacytidine solution to obtain a stock solution;
[0014] (2) Dilute the stock solution obtained in step (1) to obtain a dilution;
[0015] (3) Uniformly spray the dilution obtained in step (2) onto the plant seedlings, and carry out seedling management after spraying.
[0016] Preferably, the concentration of the stock solution in step (1) is 150 - 250 mM.
[0017] Preferably, the dilution method in step (2) is to dilute the stock solution and water at a volume ratio of 1:800 - 1200.
[0018] Preferably, the number of spraying times in step (3) is 3 - 4 times, the spraying frequency is 10 - 15 h / time, and the spraying dosage is 2 - 4 ml / time / plant; the plant is cucumber.
[0019] Preferably, the above-ground part of the plant seedlings is sprayed during spraying in step (3).
[0020] The beneficial effects of the present invention compared with the prior art are as follows:
[0021] The present invention provides the use of 5-azacytidine as a plant growth regulator. As a DNA methyltransferase inhibitor, 5-azacytidine can significantly inhibit the degree of phototropic bending of cucumber. By spraying 5-azacytidine during the cucumber seedling stage, the present invention adjusts the architecture of cucumber and improves the seedling raising efficiency of cucumber seedlings. The method for regulating plant architecture provided by the present invention has the advantages of simple operation and cost saving in cultivation. The present invention provides an efficient solution for the industrial development of cucumber seedling raising, expands a new direction for the application research of methylation inhibitors in plant architecture regulation, and provides a new method for efficient cucumber seedling raising. Description of the Drawings
[0022] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the accompanying drawings required in the embodiments. Obviously, the accompanying drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other accompanying drawings can also be obtained based on these drawings.
[0023] Figure 1 It is a schematic diagram for measuring angles, where angle 1 represents the epicotyl angle, angle 2 represents the first leaf cutting angle, angle 3 represents the second leaf cutting angle, angle 4 represents the first petiole angle, and angle 5 represents the second petiole angle;
[0024] Figure 2 It is a physical picture of the phototropic growth phenotype of cucumber seedlings after spraying 0 μM and 200 μM of 5-azacytidine nucleoside solution;
[0025] Figure 3 It is the statistical result of the epicotyl angle data of cucumber seedlings after spraying 0 μM and 200 μM of 5-azacytidine nucleoside solution;
[0026] Figure 4 It is the statistical result of the first leaf cutting angle data of cucumber seedlings after spraying 0 μM and 200 μM of 5-azacytidine nucleoside solution;
[0027] Figure 5 It is the statistical result of the second leaf cutting angle data of cucumber seedlings after spraying 0 μM and 200 μM of 5-azacytidine nucleoside solution;
[0028] Figure 6 It is the statistical result of the first petiole angle data of cucumber seedlings after spraying 0 μM and 200 μM of 5-azacytidine nucleoside solution;
[0029] Figure 7 It is the statistical result of the second petiole angle data of cucumber seedlings after spraying 0 μM and 200 μM of 5-azacytidine nucleoside solution.
[0030] In addition, Figures 3 to 7 the treatment time in is calculated since the first spraying treatment. Detailed implementation manners
[0031] Now, various exemplary implementation manners of the present invention will be described in detail. This detailed description should not be considered as a limitation of the present invention, but should be understood as a more detailed description of certain aspects, characteristics, and implementation schemes of the present invention.
[0032] It should be understood that the terms used in this invention are only for describing specific embodiments and are not intended to limit the invention. Additionally, for the numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Each intermediate value within any stated value or stated range, as well as each smaller range between any other stated value or intermediate value within the stated range, is also included in this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.
[0033] Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Although this invention only describes preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of this invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials related to those documents. In case of conflict with any incorporated document, the content of this specification shall prevail.
[0034] Without departing from the scope or spirit of this invention, various improvements and changes can be made to the specific embodiments of the description of this invention, which are obvious to those skilled in the art. Other embodiments obtained from the description of this invention are obvious to those skilled in the art. The description and examples of this invention are merely exemplary.
[0035] Regarding "comprising", "including", "having", "containing", etc. used herein, they are all open-ended terms, meaning including but not limited to.
[0036] This invention provides the use of 5-azacytidine as a plant growth regulator.
[0037] In this invention, the effective concentration of the 5-azacytidine is preferably 150 - 250 μM, more preferably 160 - 240 μM, still more preferably 180 - 220 μM, and even more preferably 200 μM; the plant regulated by the 5-azacytidine as a plant growth regulator is preferably cucumber.
[0038] This invention also provides the use of 5-azacytidine in the preparation of a product having the function of regulating plant plant type. In this use, the plant is preferably cucumber; the dosage forms of the product preferably include solution, spray, and granule.
[0039] This invention also provides a method for regulating plant plant type, including the following steps:
[0040] (1) Prepare a 5-azacytidine solution to obtain a stock solution;
[0041] (2) Dilute the mother liquor obtained in step (1) to obtain a dilution solution;
[0042] (3) Uniformly spray the dilution solution obtained in step (2) onto the plant seedlings, and perform seedling raising management after spraying.
[0043] Specifically as follows:
[0044] In the present invention, the preparation method of the mother liquor in step (1) is preferably: dissolving the 5-azacytidine in dimethyl sulfoxide (DMSO) to obtain a 5-azacytidine solution; the mass ratio of the 5-azacytidine to DMSO is preferably 1:18 - 30, more preferably 1:20 - 25, and even more preferably 1:22 - 24; that is, 15 - 23 g of DMSO is used to dissolve every 1 g of 5-azacytidine; the concentration of the mother liquor is preferably 150 - 250 mM, more preferably 160 - 240 mM, even more preferably 180 - 220 mM, and still more preferably 200 mM; the dilution method in step (2) is preferably diluting the mother liquor and water according to a volume ratio of 1:800 - 1200, more preferably according to a volume ratio of 1:900 - 1100, and even more preferably according to a volume ratio of 1:1000; the number of spraying times in step (3) is preferably 3 - 4 times; the spraying frequency is preferably 10 - 15 h / time, more preferably 12 - 14 h / time, and even more preferably 13 h / time; the spraying dosage is preferably 2 - 4 ml / time / plant, more preferably 3 ml / time / plant; the plant is preferably cucumber, and the variety of the cucumber is preferably the European greenhouse type cucumber C1717; when spraying, it is preferably to spray the above-ground part of the plant seedlings; the temperature of the seedling raising management is preferably 20 - 28 °C, more preferably 22 - 26 °C, even more preferably 24 - 25 °C; the humidity of the seedling raising management is preferably 60 - 70%, more preferably 62 - 68%, and even more preferably 64 - 66%; the light intensity of the seedling raising management is preferably 10000 - 15000 Lux, more preferably 12000 - 14000 Lux, and even more preferably 13000 Lux.
[0045] Example 1
[0046] A method for regulating plant architecture, the specific steps are as follows:
[0047] (1) Using 5-azacytidine as the solute and DMSO as the solvent, mix them according to the mass ratio of solute: solvent = 1:22.5 to obtain a mother liquor with a concentration of 200 mM; store it in an environment of -20 °C.
[0048] (2) Dilute the above-obtained mother liquor with water at a volume ratio of 1:1000, and mix well to obtain a dilution;
[0049] (3) Uniformly spray the dilution obtained in step (2) onto the above-ground parts of cucumber seedlings of European greenhouse cucumber C1717 that have grown for 20 days at a frequency of 12 h / time and 3 ml / time / plant, and spray a total of 3 times. After spraying, place the cucumber seedlings in a unilateral light environment with a temperature of 28 °C, a humidity of 70%, and a light intensity of 12000 Lux for cultivation to obtain cucumber plants.
[0050] Example 2
[0051] A method for regulating plant plant type, the specific steps are as follows:
[0052] (1) Use 5-azacytidine as the solute and DMSO as the solvent, and mix them at a mass ratio of solute: solvent = 1:18 to obtain a mother liquor with a concentration of 250 mM; store it in an environment of -20 °C;
[0053] (2) Dilute the above-obtained mother liquor with water at a volume ratio of 1:1000, and mix well to obtain a dilution;
[0054] (3) Uniformly spray the dilution obtained in step (2) onto the above-ground parts of cucumber seedlings that have grown for 20 days at a frequency of 15 h / time and 2 ml / time / plant, and spray a total of 3 times. After spraying, place the cucumber seedlings in a unilateral light environment with a temperature of 20 °C, a humidity of 60%, and a light intensity of 10000 Lux for cultivation to obtain cucumber plants.
[0055] Example 3
[0056] A method for regulating plant plant type, the specific steps are as follows:
[0057] (1) Use 5-azacytidine as the solute and DMSO as the solvent, and mix them at a mass ratio of solute: solvent = 1:30 to obtain a mother liquor with a concentration of 150 mM; store it in an environment of -20 °C;
[0058] (2) Dilute the above-obtained mother liquor with water at a volume ratio of 1:1000, and mix well to obtain a dilution;
[0059] (3) Uniformly spray the dilution obtained in step (2) onto the above-ground parts of cucumber seedlings that have grown for 20 days at a frequency of 10 h / time and 4 ml / time / plant, and spray a total of 4 times. After spraying, place the cucumber seedlings in a unilateral light environment with a temperature of 25 °C, a humidity of 65%, and a light intensity of 15000 Lux for cultivation to obtain cucumber plants.
[0060] Comparative Example 1
[0061] The cucumber seedlings were treated according to the method in Example 1, except that the 5-azacytidine nucleoside solution with a concentration of 200 μM was replaced with a 200 μM DMSO solution.
[0062] Test Example 1 Evaluation of the spraying effect of 5-azacytidine nucleoside solution
[0063] Cucumber seedlings were treated according to the methods in Example 1 and Comparative Example 1 respectively. Three replicates were set for each treatment.
[0064] After spraying three times, according to Figure 1 the marked angles, a protractor was used to accurately measure the deflection angles of cucumbers to explore the phototropic growth phenotype of cucumber seedlings. 12 cucumber seedlings were measured for each replicate. GraphPad Prism 9.0 was used to analyze and plot the data. To further compare the differences between the spraying treatment and the control, an independent samples T-test was used to statistically analyze the cucumber deflection angle data of the 0 μM 5-azacytidine nucleoside solution (Comparative Example 1) and 200 μM 5-azacytidine nucleoside (Example 1). The results are as Figures 3 to 7 shown.
[0065] The results showed that the cucumber seedlings in Comparative Example 1 sprayed with DMSO reacted more rapidly to the poor culture environment, and significant differences (p < 0.05) were observed in all 5 angles measured at 12 h; over time, the degree of angle change gradually decreased. The cucumber seedlings sprayed with the 200 μM 5-azacytidine nucleoside solution provided by the present invention reacted more gently to the poor culture environment. It can be seen that 5-azacytidine nucleoside can effectively regulate the plant type of cucumber seedlings.
[0066] From the above examples, it can be seen that the present invention provides a plant growth regulator capable of effectively regulating the plant type of cucumber seedlings. The plant growth regulator is 5-azacytidine nucleoside; the 5-azacytidine nucleoside can inhibit the phototropic growth of plants, help seedlings adapt to irregular light conditions, promote the upright and uniform growth of seedlings, reduce the cost of manual adjustment, and thus solve the problem of uneven plant type caused by phototropism during the seedling raising process.
[0067] The above is only the preferred embodiment of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and refinements can be made, and these improvements and refinements should also be regarded as the protection scope of the present invention.
Claims
Application of 1.5-azacytidine nucleoside as a plant growth regulator.
2. The use according to claim 1, characterized in that: The effective concentration of the 5-azacytidine nucleoside is 150-250 μM.
3. The use according to claim 1 or 2, characterized in that: The plant regulated by the 5-azacytidine nucleoside as a plant growth regulator is cucumber. Application of 4.5-azacytidine nucleoside in the preparation of products having the effect of regulating plant type.
5. The use according to claim 4, characterized in that: The plant is cucumber; the dosage forms of the product include solution, spray and granule.
6. A method for regulating plant type, characterized in that: The steps include: (1) preparing a 5-azacytidine solution to obtain a mother solution; (2) diluting the mother solution obtained in step (1) to obtain a diluted solution; (3) spraying the diluted solution obtained in step (2) evenly onto the plant seedlings, and performing seedling management after spraying.
7. The method for regulating plant type according to claim 6, characterized in that: The concentration of the mother solution in step (1) is 150-250 mM.
8. The method for regulating plant type according to claim 6, characterized in that: The dilution method in step (2) is to dilute the mother liquor with water in a volume ratio of 1:800 to 1200.
9. The method for regulating plant type according to claim 6, characterized in that: The number of spraying in step (3) is 3 to 4 times, the frequency of spraying is 10 to 15 hours per time, and the amount of spraying is 2 to 4 ml per time per plant; and the plant is cucumber.
10. The method for regulating plant architecture according to any one of claims 6 to 9, characterized in that: In step (3), the spraying is applied to the above-ground part of the plant seedlings.