Rabbit lumbar intervertebral disc degeneration modeling device and method

Abstract

The invention discloses a rabbit lumbar intervertebral disc degeneration molding device which comprises a base, two supports which are oppositely arranged at intervals are fixedly arranged on the base, screw rods which are vertically arranged are rotatably installed on the supports, sliding blocks are in threaded connection with the screw rods, the sliding blocks are slidably connected to the supports in the vertical direction, and the sliding blocks are in threaded connection with the supports. A bearing plate arranged in the horizontal direction is rotationally installed between the sliding blocks of the two supports, a binding structure is arranged on the upper side face of the bearing plate, elastic pieces are connected between the bearing plate and the supports, and the bearing plate is kept in the horizontal direction under the action of the elastic pieces. And a driving structure capable of simultaneously driving the two screw rods to rotate is arranged on the base. The invention further discloses a rabbit lumbar intervertebral disc degeneration modeling method using the device. The invention aims to provide the rabbit lumbar intervertebral disc degeneration molding device and method which are simple and convenient to operate.

Description

Technical Field

[0001] The present invention relates to the technical field of medical experimental techniques, and particularly to a rabbit lumbar intervertebral disc degeneration modeling device and method. Background Art

[0002] The intervertebral disc is composed of structures such as the nucleus pulposus tissue, annulus fibrosus, and cartilage endplate. There is a high positive correlation between intervertebral disc degeneration and low back pain. Intervertebral disc degeneration refers to the process of gradual deterioration and damage of the structure and function of the intervertebral disc. In the early stage of intervertebral disc degeneration, the extracellular matrix homeostasis in the nucleus pulposus is damaged due to a series of reasons, the hydration is reduced, the compressive capacity is weakened, the annulus fibrosus bears too much load and generates cracks. As the degeneration progresses, the cracks increase, and some nerves and blood vessels grow into the annulus fibrosus from the damaged cracks, and the nucleus pulposus tissue protrudes from the damaged annulus fibrosus. The abnormal local microenvironment in the degenerated intervertebral disc will stimulate the pain-sensing nerves to generate discogenic pain, and the sciatic nerve will also be compressed by the protruding nucleus pulposus tissue, causing the patient to have radiating pain and numbness in the lower body. Currently, for the clinical treatment of intervertebral disc degeneration, such as minimally invasive nucleus pulposus resection, etc., it mainly focuses on relieving pain and cannot cure the disease.

[0003] With the development of tissue engineering, it provides new possibilities for the treatment and regeneration of intervertebral disc degeneration. Selecting a suitable animal intervertebral disc degeneration model can better simulate human degeneration and explore its treatment methods. The degeneration model induced by annulus fibrosus puncture can largely simulate human intervertebral disc herniation. Rabbits have been widely used in intervertebral disc degeneration research due to their low price, fast growth cycle, easy positioning of lumbar intervertebral discs, and simple degeneration induction. However, the tools used in the existing rabbit lumbar intervertebral disc modeling surgery process are relatively complicated, and the process is also rather cumbersome, which is not conducive to popularization and application. Summary of the Invention

[0004] The technical problem to be solved by the present invention is to provide a rabbit lumbar intervertebral disc degeneration modeling device and method with simple operation.

[0005] The rabbit lumbar intervertebral disc degeneration modeling device in the present invention includes a base, two opposite and spaced-apart brackets are fixedly provided on the base, a vertically arranged screw rod is rotatably installed on the brackets, a slider is threadedly connected to the screw rod, the slider is vertically slidably connected to the brackets, a horizontally arranged bearing plate is rotatably installed between the sliders of the two brackets, a binding structure is provided on the upper side of the bearing plate, an elastic member is connected between the bearing plate and the brackets, the bearing plate is kept horizontally arranged under the action of the elastic member, and a driving structure capable of simultaneously driving the two screw rods to rotate is provided on the base.

[0006] The rabbit lumbar intervertebral disc degeneration modeling device in the present invention, wherein the bracket includes two opposite and spaced-apart support plates, both of the two support plates are arranged vertically, a top plate is fixedly connected between the upper ends of the two support plates, the screw rod is located between the two support plates, the upper end of the screw rod is rotatably installed on the top plate, and the slider is slidably connected vertically between the two support plates.

[0007] The rabbit lumbar intervertebral disc degeneration modeling device in the present invention, wherein the base is of a box structure, the base includes a box body and a box cover covering the box body, the box cover is located above the box body, the upper side surface of the box cover is fixedly provided with the bracket, the lower end of the screw rod passes through the box cover and extends into the box body, the lower end of the screw rod is rotatably installed on the box body, and the driving structure is installed on the box body.

[0008] The rabbit lumbar intervertebral disc degeneration modeling device in the present invention, wherein the driving structure includes a worm and two worm wheels, the worm is rotatably installed horizontally in the box body, the arrangement direction of the worm is the same as the direction of the line connecting the centers of the two screw rods, the two worm wheels are respectively fixedly sleeved on the two screw rods, both of the two worm wheels are located in the box body, and both of the two worm wheels are meshed with the worm.

[0009] The rabbit lumbar intervertebral disc degeneration modeling device in the present invention, wherein both ends of the bearing plate are respectively located on opposite sides of the bracket, elastic members are respectively connected between both ends of the bearing plate and the bracket, the elastic member includes an adjustment frame, opposite ends of the adjustment frame are respectively inserted with an adjustment rod, the two adjustment rods are respectively connected to the adjustment frame by threads with opposite helix directions, springs are respectively connected to the two adjustment rods, the spring on one adjustment rod is connected to the bearing plate, and the spring on the other adjustment rod is connected to the bracket.

[0010] The rabbit lumbar intervertebral disc degeneration modeling device in the present invention, wherein the binding structure is a first binding band and a second binding band, and both ends of the first binding band and the second binding band are respectively detachably connected to the upper side surface of the bearing plate.

[0011] The rabbit lumbar intervertebral disc degeneration modeling method using the above device in the present invention includes the following steps:

[0012] Step 1: Remove the elastic member from between the bearing plate and the bracket, rotate the worm to adjust the height of the bearing plate, after the height of the bearing plate reaches the requirement, rotate the adjustment frame relative to the two adjustment rods to adjust the length of the elastic member, make the length of the elastic member adapt to the height of the bearing plate, connect the elastic member between the bearing plate and the bracket, and under the action of the elastic member, the bearing plate is arranged horizontally.

[0013] Step 2: Use drugs to anesthetize the rabbit, then place the rabbit on the upper side of the support board in a side-lying manner, and use the first binding belt and the second binding belt to fix the rabbit on the support board, so that the first binding belt and the second binding belt are located on both sides of the rabbit's lumbar spine respectively.

[0014] Step 3: Shave and disinfect the rabbit's lumbar spine, then lay a sterile towel with holes on the rabbit, and make sure the holes of the sterile towel correspond to the rabbit's lumbar spine.

[0015] Step 4: Open the skin, fascia, and muscles of the rabbit's lumbar spine corresponding to the hole of the sterile towel layer by layer through the paramedian approach until the lumbar transverse process of the corresponding segment is exposed.

[0016] Step 5: Use a puncture needle with a scale mark to puncture the lumbar intervertebral disc at the angle between the transverse process and the head of the spine. If there is a clear sense of missing, the lumbar intervertebral disc is punctured. Then rotate the support plate from horizontal to inclined and then back to horizontal. During this process, use an X-ray machine to image the puncture site of the rabbit at different angles to determine the puncture position of the puncture needle. If the puncture position does not meet the requirements, correct the puncture position of the puncture needle until it meets the requirements. Continue to insert the puncture needle into the intervertebral disc. During this process, use an X-ray machine to image the puncture site of the rabbit again to determine the depth of the puncture needle into the intervertebral disc. When the depth of the puncture into the intervertebral disc reaches 5 mm, stop puncturing. At this time, rotate the needle 360 ​​degrees 5 times and stay for 30 seconds.

[0017] Step 6: Remove the puncture needle and close the incision layer by layer.

[0018] Step 7: Remove the first and second binding bands, then transfer the rabbit to the rabbit cage and continue feeding.

[0019] Step 8: Verify the intervertebral disc degeneration of the rabbit within the preset time. When the observed indicators of the rabbit's intervertebral disc show obvious changes, the puncture causes intervertebral disc degeneration and the model is successful.

[0020] In the rabbit lumbar disc degeneration modeling method of the present invention, in the step eight, after a preset time, the rabbit's lumbar spine is imaged by an X-ray machine, and the observation index is the gap between two adjacent vertebrae of the punctured intervertebral disc. When the gap becomes narrower than the initial state, the puncture causes intervertebral disc degeneration and the modeling is successful.

[0021] The method for modeling rabbit lumbar intervertebral disc degeneration in the present invention. In step eight, after a preset time, magnetic resonance imaging is performed on the lumbar spine of the rabbit. The observation index is the T2-weighted image of the punctured intervertebral disc. The T2-weighted image of the intervertebral disc of a normal rabbit is a high signal. If the T2-weighted image of the punctured intervertebral disc is a low signal, then the puncture causes intervertebral disc degeneration and the modeling is successful.

[0022] The method for modeling rabbit lumbar intervertebral disc degeneration in the present invention. In step eight, after a preset time, the rabbit is sacrificed, the punctured intervertebral disc is dissected, placed in 4% paraformaldehyde for fixation for 24 hours, and then decalcified in 15% ethylenediaminetetraacetic acid (EDTA) for 30 days. After dehydration and clearing of the decalcified intervertebral disc, it is immersed in molten paraffin for embedding. Sections are made of the embedded intervertebral disc, and the thickness of the sections does not exceed 5 μm.

[0023] Hematoxylin and eosin (HE) staining is performed on the sectioned intervertebral disc. The observation index is the histological structure of the stained intervertebral disc. If the histological structure is damaged, then the puncture causes intervertebral disc degeneration and the modeling is successful, and / or

[0024] Safranin-fast green staining is performed on the sectioned intervertebral disc. The observation indexes are the histological structure, proteoglycan content, and type II collagen content of the stained intervertebral disc. If the histological structure is damaged and the proteoglycan content and type II collagen content decrease, then the puncture causes intervertebral disc degeneration and the modeling is successful.

[0025] The difference between the device and method for modeling rabbit lumbar intervertebral disc degeneration in the present invention and the prior art is that when performing puncture modeling on the lumbar intervertebral disc of a rabbit in the present invention, the rabbit can be fixed on the bearing plate, and the height of the bearing plate can also be adjusted according to actual needs. The bearing plate is kept in the horizontal direction by the action of an elastic member. When puncturing the rabbit, it is necessary to perform imaging on the puncture site of the rabbit through an X-ray machine to observe whether the puncture needle is aligned with the intervertebral disc to be punctured. If not, the puncture position of the puncture needle is corrected until it is aligned. When the X-ray machine performs imaging on the puncture site, the bearing plate can be rotated to allow the X-ray machine to perform imaging on the puncture site of the rabbit at different angles, so as to accurately determine the position of the puncture needle. After the puncture position is determined, subsequent puncture operations can be carried out. After the puncture operation is completed, the fixation of the rabbit is released, and the rabbit is removed from the bearing plate. Finally, the degeneration of the intervertebral disc of the rabbit within the preset time is verified. It can be seen that, compared with the existing rabbit lumbar intervertebral disc modeling surgery, the operation of the present invention is more convenient.

[0026] The present invention will be further described below with reference to the accompanying drawings. Description of the Drawings

[0027] Figure 1 This is the front view of the rabbit lumbar intervertebral disc degeneration modeling device in the present invention;

[0028] Figure 2 It is along Figure 1 The sectional view taken along line A-A in;

[0029] Figure 3 This is the left view of the rabbit lumbar intervertebral disc degeneration modeling device in the present invention;

[0030] Figure 4 It is along Figure 3 The sectional view taken along line B-B in;

[0031] Figure 5 This is the top view of the rabbit lumbar intervertebral disc degeneration modeling device in the present invention;

[0032] Figure 6 This is the schematic diagram of the rabbit after being anesthetized and the lumbar region being shaved in the modeling method of the present invention;

[0033] Figure 7 This is the schematic diagram of the rabbit's lumbar region being disinfected and covered with sterile towels in the modeling method of the present invention;

[0034] Figure 8 This is the schematic diagram of the rabbit's lumbar region being incised and the lumbar transverse processes being exposed in the modeling method of the present invention;

[0035] Figure 9 This is the schematic diagram of a puncture needle being inserted into a lumbar intervertebral disc of a rabbit in the modeling method of the present invention;

[0036] Figure 10 This is the schematic diagram of three lumbar intervertebral discs of a rabbit being respectively inserted with puncture needles in the modeling method of the present invention;

[0037] Figure 11 This is the schematic diagram of the rabbit after the incision is closed in the modeling method of the present invention;

[0038] Figure 12 This is the schematic diagram of a puncture needle being punctured at the angle between the transverse process and the head end direction of the spine in the modeling method of the present invention;

[0039] Figure 13 This is the X-ray diagram of the puncture needle being inserted into the rabbit's lumbar intervertebral disc in the modeling method of the present invention;

[0040] Figure 14 It is the X-ray diagram of the rabbit's lumbar intervertebral disc 8 weeks after puncture in the modeling method of the present invention;

[0041] Figure 15 This is the MRI (magnetic resonance imaging) diagram of the rabbit's lumbar intervertebral disc 8 weeks after puncture in the modeling method of the present invention.

[0042] In the figure:

[0043] 1. Bracket, 2. Support plate, 3. Worm, 4. Handle, 5. Box cover, 6. Base, 7. Box body, 8. Bearing seat, 9. Rotating shaft, 10. Hook-and-loop fastener, 11. Bearing plate, 12. First binding strap, 13. Support ear, 14. Spring, 15. Adjusting rod, 16. Adjusting frame, 17. Connecting plate, 18. Chute, 19. Slide block, 20. Worm gear, 21. Screw rod, 22. Top plate, 23. Puncture needle, 24. Vertebral body, 25. Intervertebral disc, 26. Transverse process, 27. Sterile towel, 28. Hole in the sterile towel, 29. Second binding strap. Specific embodiments

[0044] As Figure 1 shown and in combination with Figures 2 - 5 shown, the rabbit lumbar intervertebral disc degeneration modeling device in the present invention includes a base 6. Two opposite and spaced brackets 1 are fixedly provided on the base 6. A vertically arranged screw rod 21 is rotatably installed on the brackets 1. A slide block 19 is threadedly connected to the screw rod 21. The slide block 19 is vertically slidably connected to the brackets 1. A horizontally arranged bearing plate 11 is rotatably installed between the slide blocks 19 of the two brackets 1. A binding structure is provided on the upper side of the bearing plate 11. An elastic member is connected between the bearing plate 11 and the brackets 1. The bearing plate 11 is kept horizontally arranged under the action of the elastic member. A driving structure capable of simultaneously driving the two screw rods 21 to rotate is provided on the base 6.

[0045] As Figure 2 、 3 shown, the rabbit lumbar intervertebral disc degeneration modeling device in the present invention, wherein the bracket 1 includes two opposite and spaced support plates 2. Both support plates 2 are vertically arranged. A top plate 22 is fixedly connected between the upper ends of the two support plates 2. The screw rod 21 is located between the two support plates 2. The upper end of the screw rod 21 is rotatably installed on the top plate 22. The slide block 19 is vertically slidably connected between the two support plates 2.

[0046] The structures of the two brackets 1 are exactly the same. A screw rod 21 and a slide block 19 are installed on each bracket 1. The upper end of the screw rod 21 is rotatably installed on the top plate 22 through a bearing, so that the screw rod 21 is rotatably installed on the bracket 1. The way that the slide block 19 is vertically slidably connected between the two support plates 2 is: As Figure 2 、 4As shown, on the two opposite sides of the two support plates 2, vertical sliding grooves 18 are respectively provided. On the slider 19, two oppositely arranged sliding protrusions (not shown in the figure) are fixedly provided. The two sliding protrusions are respectively slidably connected in the sliding grooves 18 of the two support plates 2. In this way, the slider 19 can slide vertically along the sliding groove 18 through the sliding protrusions, that is, the purpose of vertically slidably connecting the slider 19 to the bracket 1 is achieved. The screw 21 and the slider 19 together form a lead screw-nut pair. When the screw 21 rotates, since the slider 19 is slidably connected to the bracket 1, the slider 19 will not rotate with the screw 21, but slide vertically along the bracket 1. In the present invention, when the two screws 21 rotate in the same direction at the same time, the two sliders 19 can rise or fall simultaneously.

[0047] The way the bearing plate 11 is rotatably installed between the two sliders 19 is as follows: As Figure 4 shown and in combination with Figure 1 、 5 shown, on the left and right opposite sides of the bearing plate 11, rotating shafts 9 are respectively fixedly provided. On the two sliders 19, bearing seats 8 are respectively fixedly provided. The two rotating shafts 9 are respectively rotatably installed in the two bearing seats 8. In this way, the bearing plate 11 is rotatably installed between the two sliders 19 through the rotating shafts 9 and the bearing seats 8. As described above, when the two screws 21 rotate in the same direction at the same time, the two sliders 19 can rise or fall simultaneously, then the bearing plate 11 rotatably installed between the two sliders 19 can also rise or fall synchronously with the two sliders 19.

[0048] As Figure 2 、 4 shown, in the rabbit lumbar intervertebral disc degeneration modeling device of the present invention, the base 6 is of a box structure. The base 6 includes a box body 7 and a box cover 5 covering the box body 7. The box cover 5 is located above the box body 7. The upper side surface of the box cover 5 is fixedly provided with the bracket 1. The lower end of the screw 21 passes through the box cover 5 and extends into the box body 7. The lower end of the screw 21 is rotatably installed on the box body 7. The driving structure is installed on the box body 7.

[0049] In order to further enhance the stability of the screw 21, the lower end of the screw 21 is rotatably installed on the bottom wall of the box body 7 through a bearing.

[0050] In the rabbit lumbar intervertebral disc degeneration modeling device of the present invention, the driving structure includes a worm 3 and two worm wheels 20. The worm 3 is rotatably installed horizontally in the box body 7. The arrangement direction of the worm 3 is the same as the direction of the line connecting the centers of the two screws 21. The two worm wheels 20 are respectively fixedly sleeved on the two screws 21. The two worm wheels 20 are both located in the box body 7. The two worm wheels 20 are both meshed with the worm 3.

[0051] Both ends of the worm 3 are rotatably mounted on two opposite side walls of the box body 7 through bearings, and one end of the worm 3 extends outside the box body 7 and is fixedly connected to a handle 4. In this way, the worm 3 can be conveniently rotated by rotating the handle 4. When the worm 3 rotates, the two worm wheels 20 meshing with the worm 3 rotate in the same direction at the same time. Since the two worm wheels 20 are respectively fixedly sleeved on the two screw rods 21, the two screw rods 21 can rotate in the same direction at the same time. Specifically, when the worm 3 rotates forward, the two worm wheels 20 rotate forward at the same time, and the two screw rods 21 also rotate forward at the same time. Then the two sliders 19 drive the bearing plate 11 to move upward; on the contrary, when the worm 3 rotates reversely, the two worm wheels 20 rotate reversely at the same time, and the two screw rods 21 also rotate reversely at the same time. Then the two sliders 19 drive the bearing plate 11 to move downward.

[0052] As Figures 1 - 5 shown, in the rabbit lumbar intervertebral disc degeneration modeling device of the present invention, both ends of the bearing plate 11 are respectively located on opposite sides of the bracket 1. Elastic members are respectively connected between both ends of the bearing plate 11 and the bracket 1. The elastic member includes an adjustment frame 16. Opposite ends of the adjustment frame 16 are respectively penetrated by an adjustment rod 15. The two adjustment rods 15 are respectively connected to the adjustment frame 16 by threads with opposite helix directions. Springs 14 are respectively connected to the two adjustment rods 15. The spring 14 on one adjustment rod 15 is connected to the bearing plate 11, and the spring 14 on the other adjustment rod 15 is connected to the bracket 1. When the spring 14 on one adjustment rod 15 is connected to the bearing plate 11, an ear 13 is fixedly arranged on the bearing plate 11, and the spring 14 is connected to the ear 13; when the spring 14 on the other adjustment rod 15 is connected to the bracket 1, a connecting plate 17 is fixedly arranged on the bracket 1, and another ear 13 is fixedly arranged on the connecting plate 17, and the spring 14 is connected to the other ear 13.

[0053] As Figure 5 shown, the bearing plate 11 is a rectangular flat plate. Rotating shafts 9 are respectively fixedly arranged in the middle of the two short sides of the bearing plate 11. In this way, the two long side ends of the bearing plate 11 are respectively located on opposite sides of each bracket 1. It can also be said that the two long side ends of the bearing plate 11 being respectively located on opposite sides of each bracket 1 means that the two long side ends of the bearing plate 11 are respectively located on opposite sides of the vertical plane where the center lines of the two brackets 1 are located. For each bracket 1, elastic members are respectively connected between the upper end of the bracket 1 and the two long side ends of the bearing plate 11, and the two elastic members are respectively symmetrically arranged on opposite sides of the bracket 1.

[0054] In the present invention, the structures of the four elastic members are completely the same. The upper ends of the four elastic members are connected to the support 1 at equal heights, and the lower ends are respectively connected to the four corners of the bearing plate 11. In this way, in the initial state, the lengths of the four elastic members are equal, and the two springs 14 in each elastic member are in a proper stretched state. Since the lengths of the four elastic members are equal, the pulling forces of the four elastic members are also equal. Thus, the bearing plate 11 is arranged horizontally, which achieves the above-mentioned purpose of keeping the bearing plate 11 arranged horizontally under the action of the elastic members.

[0055] In the present invention, before adjusting the height of the bearing plate 11, it is necessary to remove the four elastic members from between the bearing plate 11 and the support 1, and then rotate the worm 3 to adjust the height of the bearing plate 11. After the height of the bearing plate 11 meets the requirements, adjust the lengths of the four elastic members to make them adapt to the height of the bearing plate 11. Here, "adapt" means that after adjusting the four elastic members to a certain equal length, then connect the four elastic members to between the bearing plate 11 and the support 1 again. At this time, the lengths of the four elastic members are equal, and the two springs 14 in each elastic member are in a proper stretched state. Since the lengths of the four elastic members are equal, the pulling forces of the four elastic members are also equal. Thus, the bearing plate 11 is arranged horizontally.

[0056] The length adjustment of the elastic member is achieved in the following way: After the elastic member is removed from between the bearing plate 11 and the support 1, rotate the adjustment frame 16 relative to the two adjustment rods 15. Since the two adjustment rods 15 are both threadedly connected to the adjustment frame 16 and have opposite thread directions, the two adjustment rods 15 move into or out of the adjustment frame 16 simultaneously, making the elastic member shorter or longer.

[0057] The bearing plate 11 is in a horizontal direction in the initial state. When the bearing plate 11 is rotated in the order of rotating from the horizontal direction to the inclined direction and then restored to the horizontal direction, for the convenience of description, the two long side ends of the bearing plate 11 are respectively denoted as the first long side end and the second long side end. There are two situations for the bearing plate 11 to be rotated from the horizontal direction to the inclined direction and then restored to the horizontal direction: The first situation is to rotate the first long side end downward and the second long side end upward at the same time, so that the bearing plate 11 is rotated from the horizontal direction to the inclined direction, and then the bearing plate 11 is rotated in the reverse direction to restore it to the horizontal direction; The second situation is to rotate the second long side end downward and the first long side end upward at the same time, so that the bearing plate 11 is rotated from the horizontal direction to the inclined direction, and then the bearing plate 11 is rotated in the reverse direction to restore it to the horizontal direction. Since the action principles of the elastic members in the above two situations are the same, the following only uses the first situation to illustrate how the elastic member works during the rotation of the bearing plate 11: When the first long side end is rotated downward and the second long side end is rotated upward at the same time, so that the bearing plate 11 is rotated from the horizontal direction to the inclined direction, the elastic member connected between the first long side end and the upper end of the bracket 1 elongates (that is, the spring 14 in the elastic member elongates to make the elastic member elongate), that is, the tension of the elastic member becomes larger, and the elastic member connected between the second long side end and the upper end of the bracket 1 shortens until it returns to the natural state (that is, the spring 14 in the elastic member shortens until it returns to the natural state to make the elastic member shorten until it returns to the natural state), that is, the tension of the elastic member becomes smaller until it becomes zero. Then, when the bearing plate 11 is restored from the inclined direction to the horizontal direction, the bearing plate 11 is rotated in the reverse direction, that is, the first long side end of the bearing plate 11 is rotated upward and the second long side end is rotated downward at the same time. At this time, the elastic member connected between the first long side end and the upper end of the bracket 1 shortens (that is, the spring 14 in the elastic member shortens to make the elastic member shorten), that is, the tension of the elastic member becomes smaller, and the elastic member connected between the second long side end and the upper end of the bracket 1 gradually elongates from the natural state (that is, the spring 14 in the elastic member gradually elongates from the natural state to make the elastic member gradually elongate from the natural state), that is, the tension of the elastic member becomes larger. When the bearing plate 11 is rotated to the horizontal direction, the tension of the elastic member connected between the first long side end and the upper end of the bracket 1 is equal to the tension of the elastic member connected between the second long side end and the upper end of the bracket 1. At this time, the bearing plate 11 is no longer rotated, so the bearing plate 11 is kept in the horizontal direction under the action of the elastic member.

[0058] As Figures 1 - 5 shown, in the rabbit lumbar intervertebral disc degeneration modeling device of the present invention, the binding structure is the first binding band 12 and the second binding band 29. The two ends of the first binding band 12 and the second binding band 29 are respectively detachably connected to the upper side surface of the bearing plate 11 through the magic tape 10. The first binding band 12 and the second binding band 29 are spaced apart by a certain distance. The first binding band 12 is used to bind the buttocks position of the rabbit, and the second binding band 29 is used to bind the shoulders of the rabbit.

[0059] like Figures 6 - 15 As shown, combined with Figures 1 - 5 As shown, the rabbit lumbar intervertebral disc degeneration modeling method using the above device in the present invention comprises the following steps:

[0060] Step 1: Remove the elastic member from between the carrier plate 11 and the bracket 1, rotate the worm 3 to adjust the height of the carrier plate 11, and when the height of the carrier plate 11 reaches the required height, rotate the adjustment frame 16 relative to the two adjustment rods 15 to adjust the length of the elastic member so that the length of the elastic member is adapted to the height of the carrier plate 11, connect the elastic member between the carrier plate 11 and the bracket 1, and under the action of the elastic member, the carrier plate 11 is arranged in the horizontal direction, as shown in FIG. Figures 1 - 5 As shown,

[0061] Step 2: Anesthetize the rabbit with drugs (such as sodium pentobarbital), then place the rabbit on the upper side of the support board 11 in a side-lying manner, and use the first binding belt 12 and the second binding belt 29 to fix the rabbit on the support board 11, so that the first binding belt 12 and the second binding belt 29 are respectively located on both sides of the rabbit's lumbar vertebrae, such as Figure 6 As shown,

[0062] Step 3: Shave the rabbit's lumbar spine and disinfect it, then lay a sterile towel 27 with holes 28 on the rabbit, and make the holes 28 of the sterile towel 27 correspond to the rabbit's lumbar spine, as shown in FIG. Figure 6 , 7 As shown,

[0063] Step 4: Open the skin, fascia and muscle of the rabbit's lumbar vertebrae corresponding to the hole 28 of the sterile towel 27 layer by layer through the paramedian approach until the lumbar transverse process 26 of the corresponding segment is exposed. Figure 8 As shown,

[0064] Step 5: If Figure 12 As shown, a puncture needle 23 with a scale mark is used to puncture the lumbar intervertebral disc 25 at the angle between the transverse process 26 and the head end of the spine. If there is an obvious sense of missing, it means that the lumbar intervertebral disc 25 has been punctured (the puncture depth is less than 5 mm at this time). Then, the carrier plate 11 is rotated in the order of turning from the horizontal direction to the inclined direction and then returning to the horizontal direction. During this process, the puncture site of the rabbit is imaged at different angles by an X-ray machine to determine the puncture position of the puncture needle 23. If the puncture position does not meet the requirements, the puncture position of the puncture needle 23 is corrected until the puncture position of the puncture needle 23 meets the requirements, and the puncture needle 23 continues to be inserted into the intervertebral disc 25. During this process, the puncture site of the rabbit is imaged again by an X-ray machine to determine the depth of the puncture needle 23 inserted into the intervertebral disc 25. After the depth of the puncture into the intervertebral disc 25 reaches 5 mm, the puncture is stopped. At this time, the needle head is rotated 360 degrees 5 times and stayed for 30 seconds. Figure 9 ,10 As shown

[0065] Step Six: Pull out the puncture needle 23 and close the incision layer by layer, as Figure 11 shown

[0066] Step Seven: Remove the first binding band 12 and the second binding band 29, and then transfer the rabbit to a rabbit cage for continued feeding.

[0067] Step Eight: Verify the degeneration condition of the rabbit's intervertebral disc 25 within the preset time. When obvious changes occur in the observation indexes of the rabbit's intervertebral disc 25, the puncture causes degeneration of the intervertebral disc 25 and the model establishment is successful.

[0068] As Figure 6 shown, in the above Step Two, when fixing the rabbit on the bearing plate 11 with the first binding band 12 and the second binding band 29, first fix one end of the first binding band 12 and the second binding band 29 on the bearing plate 11 on one side of the rabbit's back through the magic tape 10 respectively. Then, wind the other ends of the first binding band 12 and the second binding band 29 from above the rabbit to one side of the rabbit's abdomen. And the first binding band 12 binds the rabbit's buttocks position, and the second binding band 29 binds the rabbit's shoulders. Then, fix the other ends of the first binding band 12 and the second binding band 29 on the bearing plate 11 on one side of the rabbit's abdomen through the magic tape 10 respectively. In this way, the rabbit is fixed on the bearing plate 11, and the first binding band 12 and the second binding band 29 are respectively located on both sides of the rabbit's lumbar spine.

[0069] In the above Step Three, the sterile towel 27 is a prior art, and a hole 28 is opened thereon for surgical use, that is, the operator can perform a modeling operation on the rabbit's lumbar spine part exposed by the hole 28 through surgical instruments.

[0070] In the above Step Four, the corresponding segment refers to the lumbar vertebra 24 corresponding to the hole 28 of the sterile towel 27, that is, until the transverse process 26 of the corresponding segment of the lumbar vertebra is exposed, it can also be said that until the transverse process 26 of the lumbar vertebra 24 corresponding to the hole 28 of the sterile towel 27 is exposed.

[0071] In the above Step Five, as Figure 9 shown, one puncture needle 23 can be used to puncture only one lumbar intervertebral disc 25 of the rabbit. Of course, multiple puncture needles 23 can also be used to puncture multiple lumbar intervertebral discs 25 of the rabbit respectively. As Figure 10 shown, three puncture needles 23 are used to puncture three lumbar intervertebral discs 25 of the rabbit respectively.

[0072] In the above Step Five, as Figure 12As shown, to clearly illustrate how to insert the puncture needle 23 at the angle between the transverse process 26 and the cephalic direction of the spine (in the figure, C is the direction of the transverse process 26, D is the cephalic direction of the spine, and the angle between the two directions is α. Since the transverse process 26 extends towards the head of the rabbit, an angle α can be formed between the direction of the transverse process 26 and the cephalic direction of the spine), the incision at the lumbar spine part of the rabbit should be made larger than that of a normal operation to enable a clear display. The reason for inserting the puncture needle 23 through the transverse process 26 and the cephalic direction of the spine is that the incision of a normal operation is relatively small and will not directly expose the intervertebral disc 25, but only expose the transverse process 26 of the lumbar spine. Therefore, the puncture needle 23 is inserted based on the direction of the transverse process 26 and the cephalic direction of the spine, and the puncture position can be accurately determined with the aid of an X-ray machine.

[0073] In the above step five, the puncture needle 23 is a puncture needle 23 with scale markings, so that the depth of the puncture needle 23 inserted into the intervertebral disc 25 can be determined through the image formed by the X-ray machine (i.e., the X-ray diagram). When setting the scale markings on the puncture needle 23, a scale marking can be set every 1 millimeter from the tip towards the root direction, and the scale markings can be set as annular protrusions arranged circumferentially along the puncture needle 23, which can be clearly shown through the image formed by the X-ray machine, and thus the insertion depth of the puncture needle 23 can be accurately determined. When the scale marking 5 mm away from the tip contacts the outer wall of the intervertebral disc 25, it can be determined that the insertion depth of the puncture needle 23 into the intervertebral disc 25 is 5 mm. Of course, for the convenience of inserting the puncture needle 23 into the intervertebral disc 25 and pulling it out from the intervertebral disc 25, the annular protrusions do not protrude too much from the puncture needle 23, but only protrude slightly and can be shown through the image formed by the X-ray machine. When setting the scale markings, a single annular protrusion can also be set only at the position 5 mm away from the tip of the puncture needle 23. In this way, when this annular protrusion contacts the outer wall of the intervertebral disc 25, it can be determined that the insertion depth of the puncture needle 23 into the intervertebral disc 25 is 5 mm.

[0074] In the above step five, when determining the position of the puncture needle 23, the puncture part of the rabbit is imaged at different angles by the X-ray machine, so that the images at different angles can be compared to more accurately determine the position of the puncture needle 23. Similarly, when imaging the puncture part of the rabbit by the X-ray machine to determine the depth of the puncture needle 23 inserted into the intervertebral disc 25, the carrier plate 11 can also be rotated in the order from the horizontal direction to the inclined direction and then back to the horizontal direction to image the puncture part at different angles, so as to compare the images at different angles, thereby more accurately determining the depth of the puncture needle 23 inserted into the intervertebral disc 25.

[0075] The method for modeling rabbit lumbar intervertebral disc degeneration in the present invention. In step eight, after a preset time, the lumbar spine of the rabbit is imaged by an X-ray machine. The observation index is the gap between two adjacent vertebral bodies 24 of the punctured intervertebral disc 25. When the gap becomes narrower compared to the initial state, the puncture causes degeneration of the intervertebral disc 25 and the modeling is successful. The preset time is set to 8 weeks. After this 8-week preset time, the lumbar spine of the rabbit is imaged by an X-ray machine, and the X-ray image formed is as shown in Figure 14 shown. The position F in the figure is the gap between two adjacent vertebral bodies 24 of the normal intervertebral disc 25, and the position E is the gap between two adjacent vertebral bodies 24 of the punctured intervertebral disc 25. Compared with the gap at position F, the gap at position E is significantly narrowed.

[0076] The method for modeling rabbit lumbar intervertebral disc degeneration in the present invention. In step eight, after a preset time, the lumbar spine of the rabbit is imaged by a nuclear magnetic resonance device. The observation index is the T2-weighted image of the punctured intervertebral disc 25. The T2-weighted image of the normal rabbit intervertebral disc 25 is a high signal. If the T2-weighted image of the punctured intervertebral disc 25 is a low signal, the puncture causes degeneration of the intervertebral disc 25 and the modeling is successful. The preset time is set to 8 weeks. After this 8-week preset time, magnetic resonance imaging (MRI) of the lumbar spine of the rabbit is performed using a nuclear magnetic resonance device, and the obtained MRI image is as shown in Figure 15 shown. The position H in the figure is the normal intervertebral disc 25, showing a high signal, which means that the gap between two adjacent vertebral bodies 24 of the normal intervertebral disc 25 is larger, while the position G is the punctured intervertebral disc 25, showing a low signal, which means that the gap between two adjacent vertebral bodies 24 of the punctured intervertebral disc 25 is smaller.

[0077] The method for modeling rabbit lumbar intervertebral disc degeneration in the present invention. In step eight, after a preset time, the rabbit is sacrificed, and the punctured intervertebral disc 25 is dissected and fixed in 4% paraformaldehyde for 24 hours. Then it is decalcified in 15% ethylenediaminetetraacetic acid (EDTA) for 30 days. After dehydration and clearing of the decalcified intervertebral disc 25, it is immersed in molten paraffin for embedding. Sections are made of the embedded intervertebral disc 25, and the thickness of the sections does not exceed 5 μm.

[0078] The sections of the intervertebral disc 25 after sectioning are stained with hematoxylin and eosin (HE). The observation index is the histological structure of the stained intervertebral disc 25. If the histological structure is damaged, the puncture causes degeneration of the intervertebral disc 25 and the modeling is successful, and / or

[0079] The sections of the intervertebral disc 25 after sectioning are stained with safranin-fast green. The observation indexes are the histological structure, proteoglycan content, and type II collagen content of the stained intervertebral disc 25. If the histological structure is damaged and the proteoglycan content and type II collagen content decrease, the puncture causes degeneration of the intervertebral disc 25 and the modeling is successful.

[0080] In the above step eight, the preset time is set to 8 weeks. After this 8-week preset time, the sliced intervertebral disc 25 is subjected to HE staining to obtain a first staining image. From the first staining image, it can be concluded that the histological structure of the punctured intervertebral disc 25 has been damaged. Of course, for comparison, the normal intervertebral disc 25 can also be subjected to HE staining (the staining method is the same as that of the punctured intervertebral disc 25, which will not be elaborated), to obtain a second staining image. From the second staining image, it can be concluded that the histological structure of the normal intervertebral disc 25 has not been damaged.

[0081] In the above step eight, the preset time is set to 8 weeks. After this 8-week preset time, the sliced intervertebral disc 25 is subjected to safranin-fast green staining and a first staining image is obtained. From the first staining image, it can be concluded that the histological structure of the punctured intervertebral disc 25 has been damaged, and the contents of proteoglycan and type II collagen have decreased. Of course, for comparison, the normal intervertebral disc 25 can also be subjected to safranin-fast green staining (the staining method is the same as that of the punctured intervertebral disc 25, which will not be elaborated), to obtain a second staining image. From the second staining image, it can be concluded that the histological structure of the normal intervertebral disc 25 has not been damaged, and the contents of proteoglycan and type II collagen have not decreased.

[0082] The preset time in the above step eight can also be set to 4 weeks. In addition, in the above step five, an X-ray machine can be used to image the puncture site of the rabbit to determine the puncture position of the puncture needle 23 and correct it. After the puncture needle 23 is inserted into the intervertebral disc 25, the X-ray machine can also be used to image the puncture site to check whether the puncture position of the puncture needle 23 is accurate, such as Figure 13 is the X-ray image of the puncture needle 23 inserted into the lumbar intervertebral disc 25 of the rabbit.

[0083] The differences between the rabbit lumbar intervertebral disc degeneration modeling device and method of the present invention and the prior art are that when puncturing and modeling the lumbar intervertebral disc 25 of a rabbit, the present invention can fix the rabbit on the bearing plate 11, and can also adjust the height of the bearing plate 11 according to actual needs. The bearing plate 11 is kept in the horizontal direction by the action of the elastic member. When puncturing the rabbit, it is necessary to use an X-ray machine to image the puncture site of the rabbit to observe whether the puncture needle 23 is aligned with the intervertebral disc 25 to be punctured. If not, the puncture position of the puncture needle 23 is corrected until it is aligned. When the X-ray machine images the puncture site, the bearing plate 11 can be rotated to allow the X-ray machine to image the puncture site of the rabbit at different angles, so as to accurately determine the position of the puncture needle 23. After the puncture position is determined, subsequent puncture operations can be carried out. After the puncture operation is completed, the fixation of the rabbit is released, and the rabbit can be removed from the bearing plate 11. Finally, the degeneration of the intervertebral disc 25 of the rabbit within the preset time is verified. It can be seen that compared with the existing rabbit lumbar intervertebral disc 25 modeling surgery, the operation of the present invention is more convenient.

[0084] It should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "front", "rear", "left", "right", "middle", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation of the present invention.

[0085] In the description of the present invention, it should be noted that unless otherwise clearly specified and limited, the terms "connected" and "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0086] The above-described embodiments are only used to describe the preferred embodiments of the present invention, and do not limit the scope of the present invention. Without departing from the design spirit of the present invention, various deformations and improvements made by those of ordinary skill in the art to the technical solutions of the present invention should fall within the protection scope determined by the claims of the present invention.

Claims

1. A rabbit lumbar disc degeneration modeling device, characterized in that: The invention comprises a base, on which two brackets which are arranged opposite to each other and at intervals are fixedly provided, on which a screw rod arranged vertically is rotatably installed, on which a slider is threadedly connected, and which is slidably connected to the bracket along a vertical direction, and between which a bearing plate arranged horizontally is rotatably installed, and a binding structure is provided on the upper side surface of the bearing plate, and an elastic member is connected between the bearing plate and the bracket, and the bearing plate is kept in a horizontal arrangement under the action of the elastic member, and a driving structure which can drive the two screw rods to rotate at the same time is provided on the base.

2. The rabbit lumbar intervertebral disc degeneration modeling device according to claim 1, characterized in that: The bracket includes two supporting plates that are opposite and spaced apart, the two supporting plates are arranged vertically, a top plate is fixedly connected between the upper ends of the two supporting plates, the screw rod is located between the two supporting plates, the upper end of the screw rod is rotatably mounted on the top plate, and the slider is vertically slidably connected between the two supporting plates.

3. The rabbit lumbar intervertebral disc degeneration modeling device according to claim 2, characterized in that: The base is a box-type structure, and the base includes a box body and a box cover covering the box body. The box cover is located above the box body, and the bracket is fixedly provided on the upper side of the box cover. The lower end of the screw rod extends into the box body through the box cover, and the lower end of the screw rod is rotatably mounted on the box body, and the driving structure is mounted on the box body.

4. The rabbit lumbar intervertebral disc degeneration modeling device according to claim 3, characterized in that: The driving structure includes a worm and two worm wheels. The worm is installed in a box body and rotates horizontally. The arrangement direction of the worm is the same as the direction of the line connecting the centers of the two screws. The two worm wheels are fixedly mounted on the two screws respectively. The two worm wheels are both located in the box body and are meshed with the worm.

5. The rabbit lumbar intervertebral disc degeneration modeling device according to claim 4, characterized in that: The two ends of the supporting plate are respectively located on the opposite sides of the bracket, and elastic members are respectively connected between the two ends of the supporting plate and the bracket. The elastic member includes an adjustment frame, and an adjustment rod is respectively inserted at the opposite ends of the adjustment frame. The two adjustment rods are respectively connected to the adjustment frame through threads with opposite rotation directions. The two adjustment rods are respectively connected with springs, the spring on one adjustment rod is connected to the supporting plate, and the spring on the other adjustment rod is connected to the bracket.

6. The rabbit lumbar intervertebral disc degeneration modeling device according to claim 5, characterized in that: The binding structure comprises a first binding strap and a second binding strap, and both ends of the first binding strap and the second binding strap are detachably connected to the upper side surface of the bearing plate.

7. A method for modeling rabbit lumbar intervertebral disc degeneration using the device of claim 6, characterized in that: The following steps are involved: Step 1: Remove the elastic member from between the load-bearing plate and the bracket, rotate the worm to adjust the height of the load-bearing plate, and when the height of the load-bearing plate reaches the required height, rotate the adjustment frame relative to the two adjustment rods to adjust the length of the elastic member so that the length of the elastic member matches the height of the load-bearing plate, connect the elastic member between the load-bearing plate and the bracket, and under the action of the elastic member, the load-bearing plate is arranged in the horizontal direction. Step 2: Use drugs to anesthetize the rabbit, then place the rabbit on the upper side of the support board in a side-lying manner, and use the first binding belt and the second binding belt to fix the rabbit on the support board, so that the first binding belt and the second binding belt are located on both sides of the rabbit's lumbar spine respectively. Step 3: Shave and disinfect the rabbit's lumbar spine, then lay a sterile towel with holes on the rabbit, and make sure the holes of the sterile towel correspond to the rabbit's lumbar spine. Step 4: Open the skin, fascia, and muscles of the rabbit's lumbar spine corresponding to the hole of the sterile towel layer by layer through the paramedian approach until the lumbar transverse process of the corresponding segment is exposed. Step 5: Use a puncture needle with a scale mark to puncture the lumbar intervertebral disc at the angle between the transverse process and the head of the spine. If there is a clear sense of missing, the lumbar intervertebral disc is punctured. Then rotate the support plate from horizontal to inclined and then back to horizontal. During this process, use an X-ray machine to image the puncture site of the rabbit at different angles to determine the puncture position of the puncture needle. If the puncture position does not meet the requirements, correct the puncture position of the puncture needle until it meets the requirements. Continue to insert the puncture needle into the intervertebral disc. During this process, use an X-ray machine to image the puncture site of the rabbit again to determine the depth of the puncture needle into the intervertebral disc. When the depth of the puncture into the intervertebral disc reaches 5 mm, stop puncturing. At this time, rotate the needle 360 ​​degrees 5 times and stay for 30 seconds. Step 6: Remove the puncture needle and close the incision layer by layer. Step 7: Remove the first and second binding bands, then transfer the rabbit to the rabbit cage and continue feeding. Step 8: Verify the intervertebral disc degeneration of the rabbit within the preset time. When the observed indicators of the rabbit's intervertebral disc show obvious changes, the puncture causes intervertebral disc degeneration and the model is successful.

8. The method for modeling rabbit lumbar intervertebral disc degeneration according to claim 7, characterized in that: In step eight, after a preset time, the rabbit's lumbar spine is imaged by an X-ray machine, and the observation index is the gap between two adjacent vertebrae of the punctured intervertebral disc. When the gap becomes narrower than the initial state, the puncture causes intervertebral disc degeneration and the model is successful.

9. The method for modeling rabbit lumbar intervertebral disc degeneration according to claim 7, characterized in that: In step eight, after a preset time, the lumbar spine of the rabbit is imaged by a nuclear magnetic resonance device, and the observation index is the T2-weighted image of the punctured intervertebral disc. The T2-weighted image of the normal rabbit intervertebral disc is a high signal. If the T2-weighted image of the punctured intervertebral disc is a low signal, the puncture causes intervertebral disc degeneration and the model is successful.

10. The method for modeling rabbit lumbar intervertebral disc degeneration according to claim 7, characterized in that: In step eight, after a preset time, the rabbit is killed, the punctured intervertebral disc is dissected, and fixed in 4% paraformaldehyde for 24 hours, and then decalcified in 15% ethylenediaminetetraacetic acid (EDTA) for 30 days, the decalcified intervertebral disc is dehydrated and transparent, and then immersed in melted paraffin for embedding, and the embedded intervertebral disc is sliced, and the thickness of the slice does not exceed 5 μm. The sliced ​​intervertebral disc is stained with hematoxylin and eosin (HE). The observation index is the histological structure of the stained intervertebral disc. If the histological structure is destroyed, the puncture causes intervertebral disc degeneration and the model is successful, and / or The sliced ​​intervertebral disc was stained with safranin-fast green, and the observation indicators were the histological structure, proteoglycan content and type II collagen content of the stained intervertebral disc. If the histological structure was destroyed and the proteoglycan content and type II collagen content decreased, the puncture caused intervertebral disc degeneration and the model was successful.

Images