Mild moisturizing and soothing composition and preparation method thereof

Abstract

The invention discloses a mild moisturizing and soothing composition and a preparation method thereof, and belongs to the technical field of children cosmetics. The mild moisturizing and soothing composition is prepared from the following raw materials in parts by weight: 1 to 2 parts of metagen, 0.2 to 0.5 part of curcumin, 5 to 8 parts of periodate oxidized sodium alginate, 1 to 2 parts of chamomile extract, 1 to 2 parts of dipotassium glycyrrhizinate, 1 to 2 parts of bisabolol, 8 to 10 parts of witch hazel extract and 1 to 2 parts of xanthan gum. The mild moisturizing and soothing composition has the effects of updating and repairing cuticles of children, and the moisturizing durability of the skin of the children is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of children's moisturizing cosmetics, and particularly relates to a mild moisturizing and soothing composition and a preparation method thereof. Background Art

[0002] There are significant differences in the skin structure, composition, and function between infants and children and adults. The skin surface of adults and adolescents has an acidic pH value (pH < 5), and this acidic environment provides protection against microorganisms. However, in newborns, especially premature infants, the skin surface pH value tends to be neutral, which significantly reduces the skin's defense against excessive microbial growth.

[0003] The skin of infants is delicate, thin, and tender. Their stratum corneum is thinner than that of adults, and the epidermis and dermis are also relatively thinner. They have a lower skin lipid content but a higher water content, so they are more sensitive to foreign substances, vulnerable to irritation or damage by foreign substances, and more prone to skin trauma and percutaneous drug toxicity. The immature development of the epidermal barrier leads to skin dryness and may also promote an increase in transepidermal water loss, indicating that the epidermal barrier function has not yet fully developed. In addition, the immune function and excretory function of infants are not yet fully mature. Summary of the Invention

[0004] As described in the above prior art, one of the purposes of the present invention is to provide a hypoglycemic composition containing matsutake extract and polysaccharide of dendrobium officinale, and this mild moisturizing and soothing composition has the effect of renewing and repairing the stratum corneum of children, improving the persistence of skin moisturization in children.

[0005] Another purpose of the present invention is to provide a preparation method of the mild moisturizing and soothing composition. This method has simple steps and can industrially produce the mild moisturizing and soothing composition.

[0006] Another purpose of the present invention is to provide a children's face cream.

[0007] One of the purposes of the present invention is achieved by the following technical solution:

[0008] A mild moisturizing and soothing composition, the raw materials by weight include: postbiotics 1 - 2 parts, curcumin 0.2 - 0.5 parts, periodate-oxidized sodium alginate 5 - 8 parts, chamomile extract 1 - 2 parts, dipotassium glycyrrhizinate 1 - 2 parts, bisabolol 1 - 2 parts, witch hazel extract 8 - 10 parts, xanthan gum 1 - 2 parts.

[0009] Further, the raw materials by weight include: postbiotics 1 part, curcumin 0.5 part, periodate-oxidized sodium alginate 8 parts, chamomile extract 1 part, dipotassium glycyrrhizinate 1 part, bisabolol 1 part, witch hazel extract 8 parts, xanthan gum 2 parts.

[0010] Further, the postbiotics are one or two of antimicrobial peptides and short-chain fatty acids.

[0011] Further, the preparation method of the periodate-oxidized sodium alginate is as follows:

[0012] S1. Prepare a sodium alginate solution, suspend the sodium alginate in 50 mL of absolute ethanol to form a suspension; dissolve sodium periodate in 50 mL of deionized water to form a sodium periodate solution, add the sodium periodate solution to the sodium alginate solution, and the molar ratio of sodium periodate to sodium alginate monomer units is 1:1, and react in the dark at room temperature for 4 hours;

[0013] S2. Add ethylene glycol equimolar to the sodium periodate, stir for 1 hour to terminate the reaction, and obtain a reaction mixture;

[0014] S3. Precipitation and purification: Pour the reaction mixture into absolute ethanol, and the volume ratio of the reaction mixture to absolute ethanol is 1:5 to precipitate; after suction filtration, dialyze with distilled water for 24 hours to remove unreacted sodium periodate and small molecule impurities of ethylene glycol; freeze-dry the dialyzed liquid to obtain polyaldehyde sodium alginate, that is, the periodate-oxidized sodium alginate.

[0015] The second object of the present invention is achieved by the following technical solution:

[0016] A preparation method of a mild moisturizing and soothing composition, comprising the following steps:

[0017] S1. Add the xanthan gum to an appropriate amount of deionized water, stir until completely dissolved to form a colloidal solution;

[0018] S2. Add the postbiotics, curcumin, chamomile extract, dipotassium glycyrrhizinate, bisabolol and witch hazel extract in sequence, and stir evenly;

[0019] S3. Add the periodate-oxidized sodium alginate and stir until completely dispersed;

[0020] S4. Use a pH regulator to adjust the pH value of the mixed solution to 4.5 - 5.5;

[0021] S5. At room temperature, use a homogenizer to homogenize at a speed of 3000 - 3500 r / min for 3 - 5 minutes; after homogenization, cool to room temperature and then package to obtain the mild moisturizing and soothing composition.

[0022] The third object of the present invention is to provide a children's cream containing the mild moisturizing and soothing composition.

[0023] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0024] A mild moisturizing and soothing composition provided by the present invention adds postbiotics to regulate the microecological balance on the surface of children's skin and reduce the dependence on the stability of live probiotics. Curcumin is added. It has a mild nature and is not likely to irritate the skin. It is suitable for people with fragile skin, as well as skin that is prone to sensitivity, inflammation, and dullness. Curcumin can increase the expression of AQP3 and the transport of glycerol in cultured human keratinocytes, can increase the content of aquaporin 3 (AQP3) in the skin, promote the transport of water from the body to the epidermis, enhance the hydration of the skin, and reduce the loss of moisture on the surface of children's skin. Witch hazel extract is added, which can promote the tight connection of stratum corneum cells, enhance the integrity of the stratum corneum, and promote the repair of the stratum corneum. And it contains acidic components, which can regulate the pH value of the skin surface and keep it in an acidic state, thereby enhancing the skin barrier function. Xanthan gum can adsorb and release hydrogen ions through its polysaccharide structure, and can effectively regulate the pH value of the skin surface. This regulatory effect helps to maintain the acidic environment of the skin, thereby enhancing the skin barrier function. Chamomile extract, dipotassium glycyrrhizinate, and bisabolol are all components with mild nature, anti-inflammatory and soothing properties, and are suitable for sensitive skin and children's skin. They can work synergistically to reduce the possible irritation caused by witch hazel extract, while enhancing the repair and soothing effects of the skin. Oxidized sodium alginate contains aldehyde groups and can react with amino groups. Components in skin tissue fluid (such as amino acids, proteins, etc.) contain amino groups and can react with the aldehyde groups in oxidized sodium alginate to form a hydrogel with a three-dimensional network structure, which has the property of continuous moisturization. Detailed implementation manners

[0025] Next, in combination with specific embodiments, the present invention will be further described. It should be noted that, on the premise of no conflict, the following-described embodiments or technical features can be arbitrarily combined to form new embodiments.

[0026] The antimicrobial peptide was purchased from Yeasen Biotech Co., Ltd.;

[0027] The short-chain fatty acid was purchased from Apexbio Technology Co., Ltd.

[0028] Example 1

[0029] This example provides a mild moisturizing and soothing composition. The raw materials in parts by weight include: 1 part of postbiotic antimicrobial peptide, 0.5 part of curcumin, 8 parts of periodate-oxidized sodium alginate, 1 part of chamomile extract, 1 part of dipotassium glycyrrhizinate, 1 part of bisabolol, 8 parts of witch hazel extract, and 2 parts of xanthan gum.

[0030] In this example, the extraction and application of postbiotics: Postbiotics (such as short-chain fatty acids, antimicrobial peptides, etc.) produced by probiotic fermentation can directly act on the skin, regulate the microecological balance, and reduce the dependence on the stability of live probiotics.

[0031] In this embodiment, curcumin can regulate aquaporin, increase the content of aquaporin 3 (AQP3) in the skin, promote the transport of water from the body to the epidermis, and enhance the hydration of the skin.

[0032] It can activate the aryl hydrocarbon receptor (AhR) in keratinocytes, induce the expression of various epidermal differentiation genes, accelerate terminal differentiation, and increase the thickness of the stratum corneum, thereby enhancing the physical barrier of the skin.

[0033] Terminal differentiation: Terminal differentiation refers to the process by which cells reach their final functional state during development. In the epidermis, terminal differentiation involves the gradual transformation of keratinocytes into corneocytes and ultimately the formation of the stratum corneum. Accelerating terminal differentiation helps to form a mature stratum corneum more quickly and enhance the physical barrier function of the skin.

[0034] Increase in stratum corneum thickness: The stratum corneum is the outermost layer of the skin, mainly composed of dead corneocytes, which plays a role in protecting the skin from external damage. Increasing the thickness of the stratum corneum can enhance the barrier function of the skin, reduce water loss, and prevent the penetration of external irritants. By inducing the expression of epidermal differentiation genes and accelerating terminal differentiation, the formation and thickening of the stratum corneum can be promoted.

[0035] In this embodiment, the components in witch hazel extract can promote the tight junctions of stratum corneum cells, enhance the integrity of the stratum corneum, and promote the repair of the stratum corneum; by enhancing the water-locking ability of the stratum corneum, witch hazel extract can reduce water loss and keep the skin moisturized; witch hazel extract can protect Langerhans cells in the skin and enhance the immune function of the skin. Certain components in witch hazel can promote the division and renewal of keratinocytes, thus accelerating the repair of damaged skin.

[0036] In this embodiment, chamomile extract, dipotassium glycyrrhizinate, and bisabolol are all mild, anti-inflammatory, and soothing components, suitable for sensitive skin and children's skin. They can act synergistically to reduce the possible irritation caused by witch hazel extract, while enhancing the repair and soothing effects of the skin.

[0037] In this embodiment, the acidic components in witch hazel extract are as follows:

[0038] 1. Tannic acid: Witch hazel extract contains a relatively high concentration of tannic acid, which is a natural polyphenolic compound with antioxidant and anti-inflammatory properties. Tannic acid can regulate the pH value of the skin surface, keeping it acidic, thereby enhancing the barrier function of the skin.

[0039] 2. Gallic acid: Witch hazel extract also contains gallic acid, which is an organic acid with antibacterial and anti-inflammatory effects. Gallic acid can further regulate the acidic environment of the skin, helping to maintain the healthy state of the skin.

[0040] 3. Other organic acids: Witch hazel extract may also contain other organic acids, such as caffeic acid, oxalic acid, etc. These components also help to regulate the pH value of the skin.

[0041] Mechanism of action of witch hazel extract

[0042] 1. Regulate the skin pH value: The acidic components in witch hazel extract can lower the pH value of the skin surface, keeping it in an acidic state. This acidic environment helps to maintain the skin's barrier function and inhibit the growth of harmful microorganisms.

[0043] 2. Enhance the skin barrier function: By regulating the acidic environment of the skin, witch hazel extract can promote the formation of the stratum corneum and enhance the skin's barrier function.

[0044] 3. Anti-inflammatory and soothing effects: Components such as tannic acid and gallic acid have anti-inflammatory and soothing effects, which can reduce skin redness and itching.

[0045] In this embodiment, xanthan gum is a high molecular polysaccharide with strong water absorption and water retention capabilities. It can form a transparent film on the skin surface, which can lock in the skin's moisture, reduce water evaporation, and thus keep the skin moisturized.

[0046] Xanthan gum has a certain buffering capacity and can regulate the pH value of the skin surface. It helps to maintain the acidic environment (usually 4.5 - 6.0) on the skin surface by adsorbing and releasing hydrogen ions (H+) or hydroxide ions (OH-). This acidic environment helps to maintain the skin's barrier function, inhibit the growth of harmful microorganisms, and at the same time promote the normal metabolism and repair of skin cells.

[0047] Xanthan gum can effectively regulate the pH value of the skin surface by adsorbing and releasing hydrogen ions through its polysaccharide structure. This regulatory effect helps to maintain the acidic environment of the skin, thereby enhancing the skin's barrier function.

[0048] In this embodiment, periodate (such as sodium periodate) can oxidize the vicinal dihydroxy groups in sodium alginate into aldehyde groups. This oxidation process can be completed during the preparation stage. The resulting oxidized sodium alginate contains aldehyde groups and can react with amino groups. Components in skin tissue fluid (such as amino acids, proteins, etc.) contain primary amine groups and can undergo a Schiff base reaction with the aldehyde groups in oxidized sodium alginate. The pH value of the skin surface is usually between 4.5 - 6.5, which provides a suitable acidic environment for the Schiff base reaction.

[0049] In this embodiment, the preparation method of sodium alginate oxidized by periodate is as follows:

[0050] S1. Prepare a sodium alginate solution by suspending sodium alginate in 50 mL of absolute ethanol to form a suspension; dissolve sodium periodate in 50 mL of deionized water to form a sodium periodate solution, add the sodium periodate solution to the sodium alginate solution, and the molar ratio of sodium periodate to the monomer unit of sodium alginate is 1:1. React in the dark at room temperature for 4 hours;

[0051] S2. Add ethylene glycol in an amount equimolar to sodium periodate and stir for 1 hour to terminate the reaction, obtaining a reaction mixture;

[0052] S3. Precipitation and purification: Pour the reaction mixture into absolute ethanol, with the volume ratio of the reaction mixture to absolute ethanol being 1:5, to precipitate; after suction filtration, dialyze with distilled water for 24 hours to remove unreacted sodium periodate and small molecule impurities such as ethylene glycol; freeze-dry the dialyzed liquid to obtain polyaldehyde sodium alginate, that is, sodium alginate oxidized by periodate.

[0053] This embodiment also provides a preparation method of a mild moisturizing and soothing composition, including the following steps:

[0054] S1. Add xanthan gum to an appropriate amount of deionized water and stir until completely dissolved to form a colloidal solution;

[0055] S2. Add postbiotics antibacterial peptide, curcumin, chamomile extract, dipotassium glycyrrhizinate, bisabolol, and witch hazel extract in sequence and stir evenly;

[0056] S3. Add sodium alginate oxidized by periodate and stir until completely dispersed;

[0057] S4. Use a pH regulator to adjust the pH value of the mixed solution to 4.5 - 5.5;

[0058] S5. At room temperature, use a homogenizer to homogenize at a speed of 3000 - 3500 r / min for 3 - 5 minutes; after homogenization, cool to room temperature and then package to obtain a mild moisturizing and soothing composition.

[0059] Example 2

[0060] This embodiment provides a mild moisturizing and soothing composition, and the raw materials by weight include: 2 parts of postbiotics antibacterial peptide, 0.3 part of curcumin, 5 parts of sodium alginate oxidized by periodate, 1.5 parts of chamomile extract, 1.5 parts of dipotassium glycyrrhizinate, 1.5 parts of bisabolol, 9 parts of witch hazel extract, and 1 part of xanthan gum.

[0061] In this embodiment, the preparation method of sodium alginate oxidized by periodate is as follows:

[0062] S1. Prepare a sodium alginate solution by suspending sodium alginate in 50 mL of absolute ethanol to form a suspension; dissolve sodium periodate in 50 mL of deionized water to form a sodium periodate solution, and add the sodium periodate solution to the sodium alginate solution. The molar ratio of sodium periodate to the monomer unit of sodium alginate is 1:1, and react in the dark at room temperature for 4 hours.

[0063] S2. Add ethylene glycol in an equimolar amount to sodium periodate, and stir for 1 hour to terminate the reaction, obtaining a reaction mixture.

[0064] S3. Precipitation and purification: Pour the reaction mixture into absolute ethanol, with the volume ratio of the reaction mixture to absolute ethanol being 1:5, to precipitate; after suction filtration, dialyze with distilled water for 24 hours to remove unreacted sodium periodate and small molecule impurities such as ethylene glycol; freeze-dry the dialyzed liquid to obtain polyaldehyde sodium alginate, namely sodium alginate oxidized by periodate.

[0065] This example also provides a preparation method of a mild moisturizing and soothing composition, including the following steps:

[0066] S1. Add xanthan gum to an appropriate amount of deionized water, and stir until completely dissolved to form a colloidal solution.

[0067] S2. Sequentially add postbiotic antibacterial peptide, curcumin, chamomile extract, dipotassium glycyrrhizinate, bisabolol, and witch hazel extract, and stir evenly.

[0068] S3. Add sodium alginate oxidized by periodate, and stir until completely dispersed.

[0069] S4. Use a pH regulator to adjust the pH value of the mixed solution to 4.5 - 5.5.

[0070] S5. At room temperature, use a homogenizer to homogenize at a speed of 3000 - 3500 r / min for 3 - 5 minutes; after homogenization, cool to room temperature and then package to obtain a mild moisturizing and soothing composition.

[0071] Example 3

[0072] The mild moisturizing and soothing composition provided in this example includes the following raw materials by weight: 1.5 parts of postbiotic short-chain fatty acid, 0.2 part of curcumin, 7 parts of sodium alginate oxidized by periodate, 2 parts of chamomile extract, 2 parts of dipotassium glycyrrhizinate, 2 parts of bisabolol, 10 parts of witch hazel extract, and 1.5 parts of xanthan gum.

[0073] In this example, the preparation method of sodium alginate oxidized by periodate is as follows:

[0074] S1. Prepare a sodium alginate solution by suspending sodium alginate in 50 mL of absolute ethanol to form a suspension. Dissolve sodium periodate in 50 mL of deionized water to form a sodium periodate solution. Add the sodium periodate solution to the sodium alginate solution. The molar ratio of sodium periodate to the monomer unit of sodium alginate is 1:1. React in the dark at room temperature for 4 hours.

[0075] S2. Add ethylene glycol in an equimolar amount to sodium periodate and stir for 1 hour to terminate the reaction, obtaining a reaction mixture.

[0076] S3. Precipitation and purification: Pour the reaction mixture into absolute ethanol. The volume ratio of the reaction mixture to absolute ethanol is 1:5 to precipitate. After suction filtration, dialyze with distilled water for 24 hours to remove unreacted sodium periodate and small molecule impurities such as ethylene glycol. Freeze-dry the dialyzed liquid to obtain polyaldehyde sodium alginate, that is, sodium alginate oxidized by periodate.

[0077] This example also provides a preparation method of a mild moisturizing and soothing composition, including the following steps:

[0078] S1. Add xanthan gum to an appropriate amount of deionized water and stir until completely dissolved to form a colloidal solution.

[0079] S2. Sequentially add postbiotics short-chain fatty acids, curcumin, chamomile extract, dipotassium glycyrrhizinate, bisabolol, and witch hazel extract and stir evenly.

[0080] S3. Add sodium alginate oxidized by periodate and stir until completely dispersed.

[0081] S4. Use a pH regulator to adjust the pH value of the mixture to 4.5 - 5.5.

[0082] S5. At room temperature, use a homogenizer to homogenize at a speed of 3000 - 3500 r / min for 3 - 5 minutes. After homogenization, cool to room temperature and then package to obtain a mild moisturizing and soothing composition.

[0083] Comparative Example 1

[0084] Different from Example 1, this comparative example provides a mild moisturizing and soothing composition. The raw materials by weight include: 1 part of postbiotic antibacterial peptide, 0.5 part of curcumin, 8 parts of sodium alginate oxidized by periodate, 8 parts of witch hazel extract, and 2 parts of xanthan gum.

[0085] Comparative Example 2

[0086] Different from Example 1, this comparative example provides a mild moisturizing and soothing composition, and the raw materials by weight include: 1 part of postbiotic antibacterial peptide, 0.5 part of curcumin, 8 parts of sodium alginate, 1 part of chamomile extract, 1 part of dipotassium glycyrrhizinate, 1 part of bisabolol, 8 parts of witch hazel extract, and 2 parts of xanthan gum.

[0087] Comparative Example 3

[0088] Different from Example 1, this comparative example provides a mild moisturizing and soothing composition, and the raw materials by weight include: 1 part of postbiotic antibacterial peptide, 0.5 part of curcumin, 8 parts of periodate-oxidized sodium alginate, and 2 parts of xanthan gum.

[0089] Experimental Example 1

[0090] Animal test:

[0091] 1. Sample preparation: Immerse the mild moisturizing and soothing compositions of Examples 1-3 in 0.9% sodium chloride injection to prepare the test sample leaching solution, and then take 0.5 ml of the test sample leaching solution and moisten the gauze dressing patch.

[0092] 2. Twenty-seven New Zealand rabbits are randomly assigned to Examples 1-3, with 9 rabbits in each group. For each of the three types of tests, namely one-time intact skin irritation, one-time damaged skin irritation, and multiple-time intact skin irritation, there are three rabbits in each group. The back skin of each rabbit in each group is divided into two areas, which are used to test the sample and the control sample respectively, that is, Comparative Example 1 is to be conducted on all 27 New Zealand rabbits.

[0093] 3. Application: Apply the dressing patch to the corresponding area of the back skin of the New Zealand rabbit, and cover the applied area with a semi-occlusive bandage.

[0094] 4. Observation time: After applying the dressing patch for 4 hours, remove the patch, and observe the skin erythema and edema and other reaction conditions 3 hours later. The results are shown in Table 1.

[0095] 5. Result determination: Score according to the degree of skin erythema and edema to judge the irritation level of the product.

[0096] Evaluation criteria for skin irritation test

[0097] Non-irritating: The skin shows no obvious erythema, edema or other abnormal reactions.

[0098] Mild irritation: The skin shows slight erythema or mild edema, but the reaction is reversible.

[0099] Moderate irritation: The skin shows obvious erythema and moderate edema.

[0100] Severe irritation: The skin shows severe erythema, severe edema, and may even be accompanied by ulcers or bleeding.

[0101] Skin irritation tests are used to detect the irritating or corrosive effects of products on the skin. Since children's skin is delicate and their barrier function is relatively poor, the requirements for skin irritation tests are more stringent. Skin irritation tests usually include different tests such as a single full-thickness skin irritation, a single damaged skin irritation, and multiple full-thickness skin irritations. Strict criteria: For children's products, it is usually required that the product be non-irritating or mildly irritating in skin irritation tests.

[0102] Table 1

[0103]

[0104] As can be seen from Table 1, when the mild moisturizing and soothing compositions of Examples 1-3 were subjected to a single full-thickness skin irritation test and multiple full-thickness skin irritation tests, they were all non-irritating, indicating that the mild moisturizing and soothing compositions of the present invention are suitable for long-term use. And the single damaged skin irritation was mildly irritating, indicating that the mild moisturizing and soothing compositions of the present invention are suitable for children and meet the requirements of children's cosmetics; the mild moisturizing and soothing composition provided in Comparative Example 1 had moderate irritation. Compared with Examples 1-3, it shows that chamomile extract, dipotassium glycyrrhizinate, and bisabolol are all mild-natured, anti-inflammatory and soothing ingredients, which can act synergistically to reduce the possible irritation caused by witch hazel extract and are suitable for sensitive skin and children's skin.

[0105] Experimental Example 2

[0106] A children's volunteer group was recruited from the dermatology department and neonatology department of Fujian Children's Hospital. There were 90 infants and children, including 18 infants and 72 children aged 3 to 12 years. They were randomly assigned to Examples 1-3 and Comparative Examples 1-3, with 15 people in each group. Each group of children first underwent TEWL measurement, and then skin moisture content measurement was carried out in different areas of the same body part. This is because the TEWL measurement has less interference on the skin surface, while the capacitance method measurement may cause a slight contact effect on the skin surface, thus affecting the TEWL measurement result. It is recommended to leave a 10-minute interval between the two measurements to allow the skin surface to return to its natural state.

[0107] Determination of skin moisture content (capacitance method)

[0108] Principle: The moisturizing effect is evaluated by measuring the moisture content of the stratum corneum of the skin. This method is based on the dielectric constant difference between water and other substances. The higher the capacitance value, the higher the skin moisture content. It is suitable for evaluating the immediate moisturizing effect before and after the use of children's cosmetics.

[0109] Determination steps

[0110] 1. Prepare the instrument: Use the Corneometer CM825 instrument to ensure that the instrument has been calibrated and is in good condition.

[0111] 2. Clean the skin: Before the test, clean the skin of the subject and avoid using any cosmetics that may affect the test results.

[0112] 3. Place the probe: Gently place the probe of the instrument on the surface of the subject's skin, usually on the facial area.

[0113] 4. Measurement: Temperature: 22°C, Humidity: 50%RH. Turn on the instrument and record the capacitance value. The higher the capacitance value, the higher the skin moisture content. Measure each test point 3 times and take the average to improve accuracy.

[0114] 5. Data recording: Record the capacitance values before and after the test, compare the changes before and after using the cosmetics, and evaluate the moisturizing effect. The results are shown in Table 2.

[0115] 2. Determination of Trans-epidermal Water Loss (TEWL) of the skin

[0116] Principle: Measure the rate of water loss from the inside of the skin through the epidermis. The lower the TEWL value, the better the skin barrier function and the more significant the moisturizing effect. It is applicable to evaluating the long-term impact of children's cosmetics on the skin barrier function, especially to evaluate whether the product can reduce water loss.

[0117] Determination steps

[0118] 1. Prepare the instrument: Use the Tewameter TM300 instrument and ensure that the instrument is calibrated and in good condition.

[0119] 2. Clean the skin: Before the test, clean the skin of the subject and avoid using any skin care products or cosmetics that may affect the test results.

[0120] 3. Place the probe: Gently place the probe of the instrument on the surface of the subject's skin, usually on the facial area.

[0121] 4. Measurement: Turn on the instrument and record the TEWL value. The lower the TEWL value, the better the skin barrier function and the more significant the moisturizing effect. Measure each test point 3 times and take the average to improve accuracy.

[0122] 5. Data recording: Record the TEWL values before and after the test, compare the changes before and after using the cosmetics, and evaluate the moisturizing effect. The results are shown in Table 2.

[0123] Table 2

[0124]

[0125]

[0126] As can be seen from Table 2, compared with Comparative Examples 2-3, during the process of 1h-48h after the use of a mild moisturizing and soothing composition of Examples 1-3, the capacitance value is higher than that before use, and the TEWL value is lower than that before use, indicating that the skin moisture content decreases slightly but is still higher than the initial value, and the skin moisture loss decreases and the loss is slow, indicating that the mild moisturizing and soothing composition of the present invention has a long-lasting and significant moisturizing effect; a mild moisturizing and soothing composition of Comparative Example 1 is comparable to Examples 1-3. The capacitance values of the mild moisturizing and soothing compositions of Comparative Example 2 and Comparative Example 3 are lower than those before use, and the TEWL values are higher than those before use, indicating that the skin moisture content decreases rapidly and the skin moisture loss is rapid, especially in Comparative Example 3. It shows that the oxidized sodium alginate in Comparative Example 2 contains aldehyde groups and can react with amino groups. The components in skin tissue fluid (such as amino acids, proteins, etc.) contain amino groups and can react with the aldehyde groups in oxidized sodium alginate to form a hydrogel with a three-dimensional network structure, which has the performance of continuous moisturization and has a significant impact on the moisturizing effect; Witch hazel extract is added to Comparative Example 2. Witch hazel extract contains tannic acid acidic components, which can adjust the pH value of the skin surface and keep it in an acidic state, thereby enhancing the skin barrier function, and has a more significant impact on the moisturizing effect than oxidized sodium alginate.

[0127] The above embodiments are only the preferred embodiments of the present invention and cannot be used to limit the scope of protection of the present invention. Any non-substantial changes and substitutions made by those skilled in the art based on the present invention fall within the scope of protection required by the present invention.

Claims

1. A mild moisturizing soothing composition, characterized in that: The raw materials include, by weight: 1-2 parts of postbiotics, 0.2-0.5 parts of curcumin, 5-8 parts of sodium alginate oxidized by periodate, 1-2 parts of chamomile extract, 1-2 parts of dipotassium glycyrrhizinate, 1-2 parts of bisabolol, 8-10 parts of witch hazel extract, and 1-2 parts of xanthan gum.

2. A mild moisturizing soothing composition according to claim 1, characterized in that: The raw materials in parts by weight include: 1 part of postbiotics, 0.5 parts of curcumin, 8 parts of sodium alginate oxidized by periodate, 1 part of chamomile extract, 1 part of dipotassium glycyrrhizate, 1 part of bisabolol, 8 parts of witch hazel extract, and 2 parts of xanthan gum.

3. A mild moisturizing soothing composition according to claim 1, characterized in that: The postbiotics are one or two of antimicrobial peptides and short-chain fatty acids.

4. A mild moisturizing soothing composition according to claim 1, characterized in that: The preparation method of the periodate-oxidized sodium alginate is as follows: S1, preparing a sodium alginate solution, suspending the sodium alginate in 50 mL of anhydrous ethanol to form a suspension; dissolving sodium periodate in 50 mL of deionized water to form a sodium periodate solution, adding the sodium periodate solution to the sodium alginate solution, wherein the molar ratio of sodium periodate to sodium alginate monomer unit is 1:1, and reacting at room temperature in the dark for 4 hours; S2, adding ethylene glycol in an amount equimolar to the sodium periodate, stirring for 1 hour to terminate the reaction, and obtaining a reaction mixture; S3, precipitation and purification: pour the reaction mixture into anhydrous ethanol, the volume ratio of the reaction mixture to anhydrous ethanol is 1:5, and precipitate is precipitated; after filtration, dialyze with distilled water for 24 hours to remove unreacted sodium periodate and ethylene glycol small molecule impurities; freeze-dry the dialyzed liquid to obtain polyaldehyde sodium alginate, i.e., sodium alginate oxidized by periodate.

5. A method for preparing a mild moisturizing soothing composition according to any one of claims 1 to 4, characterized in that: The following steps are involved: S1. Add the xanthan gum into an appropriate amount of deionized water and stir until it is completely dissolved to form a colloidal solution; S2, sequentially adding the postbiotics, the curcumin, the chamomile extract, the dipotassium glycyrrhizinate, the bisabolol and the witch hazel extract, and stirring evenly; S3, adding the sodium alginate oxidized by periodate, and stirring until completely dispersed; S4, using a pH regulator to adjust the pH value of the mixed solution to 4.5-5.5; S5. At room temperature, use a homogenizer to homogenize at a speed of 3000-3500 r / min for 3-5 minutes; after homogenization, cool to room temperature and then package to obtain the mild moisturizing and soothing composition.

6. A children's facial cream, characterized in that: A mild moisturizing and soothing composition comprising any one of claims 1 to 4.

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