Biological stimulant containing edible vegetable oil extract and capable of resisting salt and alkali and increasing yield as well as application and use method of biological stimulant

By using a biostimulator composition containing high-active carbon-containing organic matter and nitrogen, phosphorus and potassium elements containing edible vegetable oil extracts, the problem of limited stress resistance and yield improvement effects in the prior art during the planting and growth of saline-alkali land was solved, significant improvement in saline-alkali resistance and yield resistance, and improved soil traits of saline-alkali land.

CN120208726APending Publication Date: 2025-06-27CHONGQING SUPER SCI & TECH DEV CO LTD
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Patent Information

Application Number
CN202510177661.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-02-18
Publication Date
2025-06-27

AI Technical Summary

Technical Problem

Existing biostimulators have limited and unstable effects in improving stress resistance and yield of crops during saline-alkali land cultivation and growth.

Method used

A highly active carbon-containing organic substance containing edible vegetable oil extracts, combined with nitrogen, phosphorus and potassium, is prepared to prepare a biostimulator that resists saline and alkali and increases yield. The biostimulator forms an efficient biostimulator composition by extracting oleic acid, linoleic acid, pallitic acid, polyphenols and sterols in edible vegetable oil, and adding urea nitrogen, potassium dihydrogen phosphate and potassium elements.

Benefits of technology

It significantly improves the stress resistance and yield of various crops during the planting and growth of saline-alkali land, improves the soil traits of saline-alkali land, reduces soil pH and salt content, and is natural, green, low-cost and efficient, and is suitable for a variety of common crops.

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Abstract

The invention discloses a saline-alkali-resistant and yield-increasing biological stimulant containing an edible vegetable oil extract as well as application and a use method of the biological stimulant. The biological stimulant comprises the following components in percentage by weight: 5-20% of a high-activity carbonaceous organic matter, 10-60% of nitrogen, 10-30% of phosphorus and 10-30% of potassium. The high-activity carbon-containing organic matter is extracted from edible vegetable oil; the high-activity carbon-containing organic matter comprises the following components in percentage by weight: 15-50% of oleic acid, 5-25% of linoleic acid, 5-20% of palmitic acid, 2-8% of polyphenol and 5-20% of sterol. According to the invention, the stress resistance and yield of crops planted in saline-alkali soil can be obviously and stably improved.
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Description

Technical Field

[0001] The present invention relates to the technical field of saline-alkali soil biostimulants, and in particular to a biostimulant containing edible vegetable oil extract for resisting saline-alkali and increasing yield, its application, and usage method. Background Art

[0002] Saline-alkali soil, also known as saline sodic soil, is land containing high concentrations of soluble salts and alkaline substances, and these soil conditions have an adverse impact on crop growth. According to the global saline soil distribution map released by the Food and Agriculture Organization of the United Nations (FAO) in 2021, the global area of saline soil has exceeded 833 million hectares (accounting for 8.7% of the total area of the earth), and most of it is distributed in arid and semi-arid regions of Africa, Asia, and Latin America.

[0003] The main characteristics of saline-alkali soil include: poor soil aeration, poor water permeability, poor heat and moisture retention, low enzyme activity and microbial activity, poor organic matter content, and poor fertility. High concentrations of salts have a direct toxic effect on plants, resulting in reduced water absorption and nutrient utilization ability of plant roots; the high pH value of the soil disrupts the acid-base balance and biochemical metabolic functions of plant cells; in addition, in some areas, saline-alkali soil even makes it impossible for most plants to grow, causing ecological degradation and seriously affecting the ecological environment.

[0004] Currently, the technologies for improving saline-alkali soil mainly include engineering, agronomic, chemical, and biological methods. Engineering and agronomic methods include irrigation for salt leaching, deep plowing, land leveling, surface covering, etc.; chemical methods mainly use chemical substances to reduce soil salinity and control alkalinity; while biological improvement methods focus on improving soil structure, enhancing soil fertility and water and fertilizer utilization efficiency by means of beneficial microorganisms and supplementing organic matter.

[0005] In recent years, the concept of saline-alkali soil improvement has shifted from "adapting the land to the crop" to "adapting the crop to the land", promoting agricultural development by breeding salt-tolerant crops and improving the salt tolerance of plants. This new concept not only reduces the technical difficulty and economic cost of the improvement process, but also enables more crops to grow normally and achieve a good harvest in saline-alkali soil, enhancing the agricultural production potential and economic development in saline-alkali areas.

[0006] Methods for improving the saline-alkali resistance of crops include using organic fertilizers, microbial fertilizers, and biostimulants, etc. A biostimulant is a substance or microorganism that can promote nutrient absorption by crops, improve nutrient utilization efficiency, enhance their resistance to abiotic stresses, or improve the quality and yield of crops when applied to seeds, plants, or the rhizosphere. Biostimulants do not provide nutritional components but achieve the effects of facilitating plant growth, stress resistance, and yield increase by regulating the physiological processes of plants. Biostimulants can be well-defined single molecules or mixtures with undefined properties and structures. Common biostimulant components include humic acid, seaweed extracts, chitosan oligosaccharides, and proteins / polypeptides / amino acids, etc. Patent CN114315483A discloses a humic acid water-soluble fertilizer containing seaweed extracts and its effects on crops in saline-alkali land. Patent CN102613193A provides the use of chitosan oligosaccharides for crop resistance to saline-alkali acid stress. Patent CN106631398B discloses a water-soluble soil conditioner package specifically for saline-alkali land, using biostimulants, which are one or more soluble biostimulants selected from humic acid substances, complex organic substances, beneficial chemical elements, inorganic minerals (including phosphites), seaweed extracts, chitin and chitosan derivatives, antitranspirants, or free amino acids. However, these conventional biostimulants have limited ability to improve the saline-alkali resistance and growth promotion of crops, and the effects are unstable. Summary of the Invention

[0007] The technical problem to be solved by the present invention is to provide a biostimulant containing edible vegetable oil extract for saline-alkali resistance and yield increase, and its application and usage method, which can significantly and stably improve the stress resistance and yield of various crops during the planting and growth processes in saline-alkali land.

[0008] To solve the above technical problems, in the first aspect, the biostimulant containing edible vegetable oil extract for saline-alkali resistance and yield increase provided by the present invention adopts the following technical solution: A biostimulant containing edible vegetable oil extract for saline-alkali resistance and yield increase, comprising the following components and their contents: 5-20% of highly active carbonaceous organic matter, 10-60% of nitrogen, 10-30% of phosphorus, and 10-30% of potassium; The highly active carbonaceous organic matter is obtained by extracting from edible vegetable oil; The highly active carbonaceous organic matter comprises the following components and their contents: 15-50% of oleic acid, 5-25% of linoleic acid, 5-20% of palmitic acid, 2-8% of polyphenols, and 5-20% of sterols. These components and contents are used as the judgment criteria for product quality.

[0009] By adopting the above technical solution, the highly active carbon-containing organic substances extracted from edible vegetable oil are prepared by adding the essential nutrient elements nitrogen, phosphorus and potassium for plant growth according to the above ratio. Through a large number of experiments, it is verified that the above highly active carbon-containing organic substances extracted from edible vegetable oil have a plant biostimulant effect, enabling the nitrogen, phosphorus and potassium in the composition to more fully and effectively provide important nutrients for crop growth. At the same time, experiments prove that the above highly active carbon-containing organic substances can improve the stress resistance of crops, especially the salt and alkali tolerance of crops, can promote the growth process and increase the yield of crops in saline-alkali land planting, and can improve the soil properties of saline-alkali land through the planting process. The above biostimulant composition belongs to natural green, efficient, low-cost, quick-acting and conducive to sustainable development. By acting on the crops themselves, it improves the salt and alkali tolerance of crops, enables the wish of "adapting to the land with seeds" to be implemented and is applicable to a variety of common crops. It becomes a reality that more crops can be planted and harvested in saline-alkali land, effectively enhancing the agricultural production potential and economic development in saline-alkali areas. Moreover, the planting process using the above biostimulant composition can also reduce the soil pH, reduce the soil salt content, and improve the properties of saline-alkali land soil, which has important agricultural production value and ecological environment significance. Its simple, low-cost and efficient characteristics make it have a wide application prospect in agricultural production in saline-alkali areas and have the significance of ecological environment protection.

[0010] Optionally, the process for extracting and preparing the highly active carbon-containing organic substances from edible vegetable oil includes the following steps: heating the edible vegetable oil to 240°C - 260°C, collecting the evaporated carbon-containing organic substances under a low-pressure environment of 2 - 3 mmHg, and then obtaining the highly active carbon-containing organic substances through cooling by a condenser.

[0011] By adopting the above technical solution, through the above method and its parameters, the above highly active carbon-containing organic substances are extracted from edible vegetable oil.

[0012] Optionally, the edible vegetable oil is one or several of corn oil, soybean oil, peanut oil, sunflower oil, rapeseed oil, olive oil, coconut oil, grape seed oil and rice bran oil.

[0013] By adopting the above technical solution, by using the above edible vegetable oil as the extraction raw material, the above highly active carbon-containing organic substances can be extracted.

[0014] In the second aspect, the application of the biostimulant for resisting salt and alkali and increasing yield containing the extract of edible vegetable oil adopts the following technical solution: Optionally, it is directly applied to seeds, seedlings and the leaves of crops at all stages of the growth process.

[0015] By adopting the above technical solutions, the above biological stimulant composition can directly act on the crop itself and can act on each growth stage of the crop, so that the crop can adapt to saline-alkali land throughout the growth period, without spending a large amount of time and economic costs in the early stage of planting to improve the soil condition, improving the planting efficiency, and effectively enhancing the agricultural production potential and economic development in saline-alkali areas.

[0016] Optionally, the crops suitable for planting in saline-alkali land include rice, cotton, wheat, corn, beans, fruits and melons, green leafy vegetables, apples, peaches, pears, berries, cruciferous vegetables, forage grasses and Chinese medicinal materials.

[0017] By adopting the above technical solutions, the above biological stimulant can be applied to the planting of various crops in saline-alkali land and increase the yield, having broad-spectrum applicability to target crops.

[0018] In the third aspect, the application of the biological stimulant containing edible vegetable oil extract for resisting saline-alkali and increasing yield adopts the following technical solutions: A method for using a biological stimulant containing edible vegetable oil extract for resisting saline-alkali and increasing yield: including seed dressing method, foliar spraying method and / or sprinkler irrigation and drip irrigation method; Seed dressing method: After diluting the said biological stimulant with water, evenly spray it on the surface of the seeds, and sow after drying. Foliar spraying method: After the seeds emerge, dilute the said biological stimulant with water, and then spray it on the leaves with a sprayer until the leaves are wet to the extent that the droplets are about to fall but have not fallen. Sprinkler irrigation and drip irrigation method: During the growth period of the crop, apply it by sprinkler irrigation or drip irrigation.

[0019] By adopting the above technical solutions, by spraying and stirring the seeds, spraying the leaves of the crop, and applying it by sprinkler irrigation or drip irrigation, the above biological stimulant can play its role, and the operation is simple and convenient, making it have a wide application prospect in agricultural production in saline-alkali areas.

[0020] Optionally, in the seed dressing method and the foliar spraying method, the biological stimulant and water are both prepared in the ratio of (10 - 20) g : (10 - 20) kg.

[0021] By adopting the above technical solutions, preparing and applying according to the above ratio makes the concentration of the biological stimulant in the irrigation solution moderate, and the effect of enhancing the stress resistance and yield of the crop is relatively better.

[0022] Optionally, in the sprinkler irrigation and drip irrigation method, the dosage of the biological stimulant for each mu of crop is 50 - 100 g.

[0023] Optionally, for severely saline-alkali land with sodium salt content ≥ 0.6% and pH ≥ 9.50, slaked lime (calcium hydroxide) is used before sowing. The dosage is 20 - 50 kg / mu, which is evenly spread on the ground surface and then plowed and mixed, or 20 - 50 kg / mu of slaked lime is mixed with 50 - 100 tons of water for irrigation.

[0024] By adopting the above technical solution, for saline-alkali land with a relatively high degree of salinity, the soil adaptability is adjusted, and the soil is treated with slaked lime, so that the biostimulant can play a better role.

[0025] Optionally, it can be used in each growth period of the crop. It is preferably sprayed on the leaf surface during the seedling stage, after transplanting, before flowering, after flowering, and during the fruit swelling stage.

[0026] Optionally, after the first application, it is applied again at intervals of at least 10 - 14 days. The biostimulant is applied 1 - 3 times during the entire growth period of the crop, and the concentration of each application can be the same as or different from that of the first application.

[0027] In summary, the present invention includes at least one of the following beneficial technical effects: 1. The above biostimulant composition is natural, green, efficient, low-cost, quick-acting and conducive to sustainable development. By acting on the crop itself, it improves the crop's salt-alkali tolerance, enabling the implementation of the wish of "adapting crops to the land" and being applicable to a variety of common crops. It becomes a reality that more crops can be planted and harvested in saline-alkali land, effectively enhancing the agricultural production potential and economic development in saline-alkali areas; 2. The planting process using the above biostimulant composition can also reduce the soil pH and soil salt content simultaneously, improve the properties of saline-alkali soil, and has important agricultural production value and ecological environment significance. Its simple, low-cost and efficient characteristics make it have a wide application prospect in agricultural production in saline-alkali areas and have the significance of ecological environment protection; 3. The above biostimulant composition can directly act on the crop itself and act on each growth period of the crop, so that the crop can tolerate saline-alkali land throughout the growth period, without spending a large amount of time and economic cost in the early stage of planting to improve the soil condition, improving the planting efficiency, and effectively enhancing the agricultural production potential and economic development in saline-alkali areas. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 It is a schematic diagram for showing the influence of foliar spraying of the biostimulant in the present invention on the growth of rice in saline-alkali land.

[0029] Figure 2 It is a schematic diagram for showing the influence of foliar spraying of the biostimulant in the present invention on the growth of Astragalus membranaceus in saline-alkali land.

[0030] Figure 3It is a schematic diagram for showing the influence of foliar spraying of the biostimulant on the growth of alfalfa in saline-alkali land in the present invention. Detailed implementation mode

[0031] To better understand the essence of the present invention, the implementation scheme of the present invention will be described in detail below in combination with embodiments. However, those skilled in the art will understand that the following embodiments are only used to illustrate the present invention and should not be regarded as limiting the scope of the present invention. The specific conditions not specified in the embodiments are carried out according to conventional conditions or the conditions recommended by the manufacturer. The reagents or instruments not specified in the manufacturer are all conventional products that can be obtained through commercial purchase.

[0032] 1. Active ingredients of the biostimulant: The biostimulant is a substance or microorganism that can promote crop nutrient absorption, improve nutrient utilization efficiency, enhance its resistance to abiotic stress, or improve crop quality and yield when used on seeds, plants or rhizosphere.

[0033] The active ingredients of the biostimulant provided by the present invention are derived from edible vegetable oil extracts. The edible vegetable oil is one or several of corn oil, soybean oil, peanut oil, sunflower oil, rapeseed oil, olive oil, coconut oil, grape seed oil and rice bran oil. The effective active ingredients are highly active small molecule carbon-containing organic substances, including oleic acid, linoleic acid, palmitic acid, polyphenols and sterols. It also contains unknown biostimulant components, which cannot be inferred and listed by current knowledge, but their activity is also very important. Purchasing pure products of the above-known active ingredients and mixing them is not sufficient to replicate all the activities of vegetable oil extracts.

[0034] 2. Content of known components of the biostimulant composition: The biostimulant composition of the present invention is prepared by using 5-20% of highly active carbon-containing organic substances derived from edible vegetable oil extracts, adding 10-60% of urea nitrogen (N) which is an essential nutrient element for plants, 10-30% of phosphorus (P2O5) provided by potassium dihydrogen phosphate, and 10-30% of potassium (K2O).

[0035] The highly active carbon-containing organic substances include but are not limited to the following components and their contents (the percentages are all mass percentages): oleic acid 15-50%, linoleic acid 5-25%, palmitic acid 5-20%, polyphenols 2-8%, sterols 5-20%. The contents of these components are the quality judgment indexes of carbon-containing organic substances. For severely saline-alkali land with sodium salt ≥ 0.6% and pH ≥ 9.50, before sowing, slaked lime (calcium hydroxide, Ca(OH)2) is used, with a dosage of 20-50 kg per mu of slaked lime spread on the ground surface and then plowed and mixed, or 20-50 kg per mu of slaked lime is mixed with 50-100 tons of water for irrigation.

[0036] 3. Target Crops: The biostimulant of the present invention is applicable to various crops cultivated in saline-alkali land, preferably rice, cotton, millet, wheat, corn, peanuts, fruits, green leafy vegetables, legumes, apples, peaches, pears, berries, cruciferous vegetables, various forages, Chinese medicinal materials, etc. The Chinese medicinal materials include, but are not limited to, Astragalus membranaceus and Medicago sativa.

[0037] 4. Application Methods: Seed Dressing: Use 10 - 20 g of the biostimulant, add 10 - 20 kg of water, spray and wet the seed surface, stir evenly, and sow after drying.

[0038] Foliar Spraying: Use 10 - 20 g of the biostimulant, add 10 - 20 kg of water, and spray on the leaves through a manual backpack sprayer.

[0039] Sprinkler Irrigation or Drip Irrigation: During the growth period of the crops, apply by sprinkler irrigation or drip irrigation, with an application rate of 50 - 100 g per mu.

[0040] 5. Application Period: It can be used in all growth stages of the crops. Preferably, conduct foliar spraying during the seedling stage, after transplanting, before flowering, after flower withering, and during fruit swelling. After the first application, it can be reapplied every 10 - 14 days or longer. The biostimulant can be applied 1 - 3 times during the entire growth period of the crops, and the concentration of each application can be the same as or different from the first application.

[0041] 6. Mechanism of Action: It includes nutritional and functional aspects, providing organic matter and nutrients of nitrogen, phosphorus, and potassium; having a plant biostimulant effect, enhancing the stress resistance of crops, especially the ability to resist saline-alkali, promoting crop growth, shortening the growth period, and increasing yield, and can improve the soil properties of saline-alkali land during the planting process.

[0042] Example 1: A biostimulant for resisting saline-alkali and increasing yield containing edible vegetable oil extract, including the following components and their contents (percentages are all by mass): 10% of highly active carbon-containing organic matter, 46.0% of urea nitrogen (N), 18% of phosphorus (P2O5) provided by potassium dihydrogen phosphate, and 11% of potassium (K2O).

[0043] The highly active carbon-containing organic matter is obtained by extracting from edible vegetable oil. The extraction process includes the following steps: Heat corn oil to 250°C, collect the evaporated carbon-containing organic substances under a low-pressure environment of 3 mmHg, and obtain the highly active carbon-containing organic matter after cooling through a condenser; The highly active carbon-containing organic matter includes the following components and their contents (percentages are all by mass): 35% of oleic acid, 20% of linoleic acid, 12% of palmitic acid, 3.5% of polyphenols, and 10% of sterols. The contents of these components serve as the current product quality judgment criteria.

[0044] The target crop is rice.

[0045] Usage method: 10 g of the above biological stimulant is mixed with 20 kg of water and sprayed on the leaf surface 3 times with a knapsack sprayer, with a spraying interval of 30 days each time.

[0046] Performance test: In Kashgar City, Xinjiang Uygur Autonomous Region, the pH of the paddy field soil is 8.50, the salt content is 2.30 g / kg, the soil organic matter is 23.7 g / kg, the available nitrogen is 143 mg / kg, the available phosphorus is 26 mg / kg, and the available potassium is 186 mg / kg. In April 2024, rice seeds were soaked in prochloraz for 48 hours, and then washed with clean water and soaked and germinated. The seedlings were transplanted and planted when they grew to the three-leaf and one-heart stage.

[0047] Normal medicine and fertilizer management: On May 9, the base fertilizer diammonium phosphate (18-46-0) was applied at a rate of 20 kg / mu. On May 25, the top dressing urea + compound fertilizer (38-26-40) 5 + 5 kg / mu was applied. On June 1, a water-soluble fertilizer (effective content: amino acid 10%, humic acid 5%) was mixed with water at a rate of 10 kg / mu and flowed into the field. On June 9, the returning green fertilizer urea + compound fertilizer (N-P5O2-K2O: 38-26-40) was applied at a rate of 5 + 5 kg / mu by broadcasting. On June 23, the panicle fertilizer urea + compound fertilizer (N-P5O2-K2O: 38-26-40) was applied at a rate of 5 + 15 kg / mu by broadcasting. On July 16, the panicle fertilizer potassium fertilizer (K2O: 36) + urea was applied at a rate of 8 + 5 kg / mu by broadcasting. Other field management measures include: On June 15, a herbicide mixture, Daojiajun (effective content: 3% pyriminobac-methyl + 20% cyhalofop-butyl + 2% penoxsulam) and Kuolaoban (effective content: 5% MCPA + 33% bentazone) were sprayed at a rate of 150 mL / mu. On July 10, the paddy field was supplementary sprayed with herbicides, 40 mL / mu of 30% cyhalofop-butyl + 180 mL / mu of 34% propanil + 60 mL / mu of 10% clomazone + 60 mL / mu of 50% butachlor.

[0048] Example 1 group: 2.2 mu of land. On the basis of normal medicine and fertilizer management, a biological stimulant containing edible vegetable oil extract for anti-salinity and yield increase was applied according to Example 1.

[0049] Control group 1: 3 mu of land, with normal medicine and fertilizer management, and no biological stimulant was applied.

[0050] The effective panicle number, grains per panicle, seed setting rate and 1000-grain weight of the Example 1 group and the Control group 1 were measured and estimated, and the results are shown in Table 1.

[0051] Table 1:

[0052] As can be seen from Table 1, the effective panicle number, grains per panicle, seed setting rate and 1000-grain weight in the Example 1 group all increased compared with those in the Control 1 group. The estimated yield per mu was 762.3 kg / mu, showing a 15.1% increase compared with 662.1 kg / mu in the control group, and the difference was very significant (P≤0.01). At the same time, combined with Figure 1 It can be seen that the growth and yield of rice using the biostimulant of the present application are significantly better than those of rice without using the biostimulant of the present application.

[0053] Example 2: A biostimulant containing an edible vegetable oil extract for combating salinity and increasing yield, which is different from Example 1 in that the target crop is Astragalus membranaceus.

[0054] Performance test: In July 2024, in the saline-alkali land of Beimen Village, Tuoketuo County, Inner Mongolia, with a pH of 9.53 and a soil salt content of 0.35%, when planting Astragalus membranaceus, there were phenomena of weak growth, withered leaves and rotten roots.

[0055] Example 2 group: Apply the biostimulant containing an edible vegetable oil extract for combating salinity and increasing yield to Astragalus membranaceus.

[0056] Control 2 group: Do not apply the biostimulant containing an edible vegetable oil extract for combating salinity and increasing yield to Astragalus membranaceus.

[0057] Measure and estimate the fresh root weight per plant and yield per mu of Astragalus membranaceus in the Example 2 group and the Control 2 group. The results are shown in Table 2.

[0058] Table 2:

[0059] As can be seen from Table 2, compared with the Control 2 group, the fresh root weight per plant in the Example 2 group increased by 23.87%, and the yield per mu increased by 24.83%. And combined with Figure 2 , Figure 2 In the upper left figure in the middle is the situation before use, the upper right figure is the situation 10 days after use, the lower left figure is the situation before harvest, and the lower right figure is the situation after harvest. From Figure 2 It can be seen that after using the biostimulant of the present application, the leaves of Astragalus membranaceus turned green within 10 days, and the roots grew strong; when harvesting the underground part of the plants on November 8, 2024, the underground part of Astragalus membranaceus in the Example 2 group was significantly thicker and had more branches than that in the Control 2 group.

[0060] Example 3: A biostimulant containing an edible vegetable oil extract for combating salinity and increasing yield, which is different from Example 1 in that the target crop is Medicago sativa.

[0061] Performance test: On July 16, 2024, at the alfalfa planting base in Longshengzhuang, Fengzhen City, Inner Mongolia, the soil had a pH of 9.25 and a salt content of 0.31%.

[0062] Example Group 3: For 20 mu of alfalfa in the flowering stage, it was foliar sprayed once with a biostimulant for saline-alkali resistance and yield increase containing edible vegetable oil extract, with a dosage of 10 g / mu.

[0063] Control Group 3: Alfalfa in the flowering stage was not treated with the biostimulant for saline-alkali resistance and yield increase containing edible vegetable oil extract.

[0064] On September 13, 2024, about two months later, the alfalfa was harvested for yield measurement and sampled for inspection. The plant height and protein content were measured, and at the same time, soil samples were collected for trait analysis. The analysis results are shown in Table 3.

[0065] Table 3:

[0066] Combined with Table 3, it can be seen that the average plant height of alfalfa in Control Group 3 was 62 cm, while the average plant height of alfalfa in Example Group 3 was 76 cm, an increase of 22.58%. The yield of alfalfa in Control Group 3 was 950 kg / mu, and the yield in Example Group 3 was 1230 kg / mu, with a yield increase of 29.5%. The protein content of alfalfa in Control Group 3 was 15.60%, and the protein content of alfalfa in Example Group 3 was 19.62%, an increase of 25.77%. And combined with Figure 3 , Figure 3 in which the left side shows the non-use situation and the right side shows the situation after use. From Figure 3 it can be seen that the alfalfa without using the biostimulant of the present application had yellowish leaves, grew sparsely, had more weeds, and was relatively shorter; while the alfalfa using the biostimulant of the present application had dark green leaves, grew densely, had fewer weeds, and was relatively taller. Further, it can also be seen from Table 3 that the soil traits of Example Group 3 were improved, the pH value decreased from 9.25 of the soil in Control Group 3 to 9.08, and the soil salt content decreased from 0.31% of the control group to 0.27%.

[0067] In summary, the following conclusions can be drawn: The biostimulant composition of the present invention can effectively improve the stress resistance and yield of crops in saline-alkali land, and improve the quality and nutrient content of agricultural products. Crops using the biostimulant composition of the present invention are easy to grow normally in saline-alkali land, enabling "planting to suit the land", making it a reality that more crops can be planted and harvested in saline-alkali land, and reducing the soil pH and soil salt content during the planting process, improving the traits of saline-alkali land soil, which has important agricultural production value and ecological environment significance. Its simple, low-cost, and high-efficiency characteristics make it have broad application prospects in agricultural production in saline-alkali areas and have the significance of ecological environment protection.

[0068] The above are all preferred embodiments of the present invention, and the protection scope of the present invention is not limited thereby. Therefore, all equivalent changes made according to the products, methods, and principles of the present invention shall be covered within the protection scope of the present invention.

Claims

1. A salt-alkali resistant and yield-increasing biostimulant containing edible plant oil extract, characterized in that: It includes the following components and their contents: 5-20% highly active carbon-containing organic matter, 10-60% nitrogen, 10-30% phosphorus and 10-30% potassium; The highly active carbon-containing organic matter is extracted from edible vegetable oil; The highly active carbon-containing organic matter comprises the following components and their contents: 15-50% of oleic acid, 5-25% of linoleic acid, 5-20% of palmitic acid, 2-8% of polyphenols and 5-20% of sterols.

2. The salt-alkali resistant and yield-enhancing biostimulant containing edible plant oil extract according to claim 1, characterized in that: The process of extracting and preparing the high-activity carbon-containing organic matter from edible vegetable oil comprises the following steps: heating the edible vegetable oil to 240°C-260°C, collecting evaporated carbon-containing organic matter under a low pressure environment of 2-3 mmHg, and then cooling it through a condenser to prepare the high-activity carbon-containing organic matter.

3. A salt-alkali resistant and yield-enhancing biostimulant containing edible plant oil extract according to any one of claims 1 or 2, characterized in that: The edible vegetable oil is one or more of corn oil, soybean oil, peanut oil, sunflower oil, rapeseed oil, olive oil, coconut oil, grape seed oil and rice bran oil.

4. An application of a salt-alkali resistant and yield-enhancing biostimulant containing an edible plant oil extract as claimed in any one of claims 1 to 3, characterized in that: Apply directly to seeds, seedlings and leaves at all stages of the growth process.

5. The use of a salt-alkali resistant and yield-increasing biostimulant containing edible plant oil extract according to claim 4, characterized in that: Crops suitable for planting in saline-alkali land include rice, cotton, wheat, corn, beans, melons and fruits, green leafy vegetables, apples, peaches and pears, berries, cabbage vegetables, forage grass and Chinese medicinal materials.

6. A method for using the salt-alkali resistant and yield-enhancing biostimulant containing edible plant oil extracts as claimed in any one of claims 1 to 3, characterized in that: This includes seed dressing, foliar spraying and / or sprinkler and drip irrigation; Seed dressing method: add water to the biostimulant and then spray it evenly on the surface of seeds, dry them and then plant them; Foliar spraying method: after the seeds have germinated, the biostimulant is diluted with water and then sprayed on the leaves with a manual or mechanical sprayer, the leaves are wetted to the extent that the droplets are about to fall but do not fall to the ground; Sprinkler irrigation and drip irrigation: Sprinkler irrigation or drip irrigation is used during the crop growth period.

7. The method for using a salt-alkali resistant and yield-increasing biostimulant containing edible plant oil extract according to claim 6, characterized in that: In the seed dressing method and the foliar spraying method, the biostimulant and water are mixed in a ratio of (10-20) g: (10-20) kg; in the sprinkler irrigation and drip irrigation method, the amount of the biostimulant used per mu of crop is 50-100 g.

8. The method for using a salt-alkali resistant and yield-increasing biostimulant containing edible plant oil extract according to claim 6, characterized in that: For severely saline-alkali land with sodium salt content ≥0.6% and pH ≥9.50, use slaked lime (calcium hydroxide) before sowing, with a dosage of 20-50kg / mu, spread on the surface and then plow and mix, or mix 20-50kg / mu of slaked lime with 50-100 tons of water for irrigation.

9. The method for using a salt-alkali resistant and yield-increasing biostimulant containing edible plant oil extract according to claim 6, characterized in that: It can be used in all growth stages of crops, preferably for foliar spraying during the seedling stage, after transplanting, before flowering, after flowering and during the fruit expansion stage.

10. The method for using the salt-alkali resistant and yield-enhancing biostimulant containing edible plant oil extract according to claim 9, characterized in that: After the first application, apply again at intervals of at least 10-14 days, for a total of 1-3 applications during the entire growth period of the crop. The concentration of each application may be the same as or different from the first application.

Citation Information

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