Pathological tissue slice quality control method and device

Abstract

The invention provides a pathological tissue section quality control method and device. The method comprises the following steps: S1, obtaining a wax block and a pathological section; s2, assigning the same unique identification codes to the wax blocks and the pathological sections corresponding to the wax blocks, and storing the wax blocks and the pathological sections in a sample library; s3, pathological sections and wax blocks to be subjected to quality inspection are selected from the constructed sample library; s4, identifying the unique identification codes of the pathological section and the wax block to be subjected to quality inspection; s5, when the unique identification code identification results of the pathological section to be subjected to quality inspection and the wax block are the same, entering step S6, otherwise, defining the pathological section to be subjected to quality inspection and the wax block to be subjected to quality inspection as abnormal samples; s6, respectively scanning the pathological section to be subjected to quality inspection and the wax block to obtain a pathological section scanning result and a wax block scanning result; and S7, comparing a pathological section scanning result with a wax block scanning result by using AI, and performing evaluation. According to the quality control method provided by the invention, the information consistency of the wax block and the pathological section and the section quality are improved, and the quality control efficiency is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of medical pathological data processing, and particularly relates to a pathological tissue section quality control method and device. Background Art

[0002] A wax block is a method of fixing and embedding biological tissue samples in a waxy matrix. The general process is as follows: Tissue blocks taken from the human body or animal body are immersed in a chemical fixative to fix the cell components; then the fixed tissue is dehydrated and impregnated with wax and embedded in a solidified wax block. Wax block making technology is widely used in histopathology, cytology and immunohistochemical analysis. A pathological section is a thin slice specimen cut from a wax block, and thin sections with different thicknesses ranging from 0.5 to 5 microns are obtained by cutting the wax block. After sectioning, staining is carried out, and common staining methods include HE staining, special staining, etc. Pathological sections can clearly present the structural characteristics of cells and tissues and are used for disease diagnosis. Therefore, pathological section analysis is one of the important means for pathologists to diagnose diseases.

[0003] During the process of pathological analysis and research, the quality of pathological sections is very important. Once there are quality problems with pathological sections, it will, at the very least, increase the difficulty of the case analysis process, and at the worst, lead to a decline or even loss of the accuracy of the case analysis results. In the prior art, for example, patent document CN111337495A provides a whole-process quality control device for pathological section images, including: a glass slide detection unit, a scanning layer selection unit, and an image quality scoring unit, which are electrically connected in sequence; the glass slide detection unit is used to detect and calculate the current glass slide information for the scanning layer selection unit to automatically select or specifically increase the scanning layer for scanning. After the scanning is completed, the image quality scoring unit introduces the CTF pointer as an imaging quality control evaluation technology to evaluate the quality of the whole slide. Another example is patent document CN114511547A, which provides a method, device, equipment, and storage medium for quality control of pathological section images. The method includes obtaining a pathological section image and a pathological section report; the pathological section image is associated with a section image category; the pathological section report includes section image classification information; obtaining a preset section image quality control model corresponding to the section image category, and inputting the pathological section image into the preset section image quality control model to obtain a first image quality control result corresponding to the pathological section image; obtaining a preset section image recognition model, and inputting the pathological section image into the preset section image recognition model to obtain a section image classification feature corresponding to the pathological section image; determining a second image quality control result corresponding to the pathological section image according to the section image classification information and the section image classification feature; and determining a section image quality control result corresponding to the pathological section image according to the first image quality control result and the second image quality control result. It can be seen that in the above technologies, quality control is carried out during the sectioning process, and there is no involvement in information verification and quality control of section quality.

[0004] In fact, the above-mentioned conventional pathological section quality control mostly relies on manual inspection. This quality inspection method has a large human interference factor and low quality inspection efficiency. Therefore, there is an urgent need for an efficient pathological section quality inspection technology. Summary of the Invention

[0005] Aiming at the deficiencies of the prior art, the purpose of the present invention is to provide a method and device for quality control of pathological tissue sections, which improves the consistency of the information of wax blocks and pathological sections and the section quality, and improves the quality control efficiency.

[0006] To achieve the above purpose, the present invention adopts the following technical solutions:

[0007] In the first aspect, the present invention provides a method for quality control of pathological tissue sections, including the following steps:

[0008] S1. Obtain wax blocks and pathological sections;

[0009] S2. Assign the same unique identification code to the wax block and its corresponding pathological section, and store them in the sample library;

[0010] S3. Select the pathological section and wax block to be quality inspected in the sample library constructed in step S2;

[0011] S4. Identify the unique identification codes of the pathological section and wax block to be quality inspected in step S3;

[0012] S5. When the identification results of the unique identification codes of the pathological section and wax block to be quality inspected are the same, proceed to step S6; otherwise, define the pathological section and wax block to be quality inspected as abnormal samples;

[0013] S6. Scan the pathological section and wax block to be quality inspected respectively to obtain the pathological section scan result and wax block scan result;

[0014] S7. Use AI to compare the pathological section scan result with the wax block scan result, and evaluate the pathological section to be quality inspected based on the comparison result.

[0015] As an optimization of the technical solution of the present invention, in step S2, the unique identification code is used to store identity information and sample data, the identity information at least includes an identity serial number, and the sample data at least includes one or more of sample source, preparation time, and sample content.

[0016] As an optimization of the technical solution of the present invention, step S5 further includes:

[0017] After the pathological section and wax block to be quality inspected are defined as abnormal samples, store the abnormal samples in the abnormal area of the sample library for storing all abnormal samples, and after completing the quality inspection of all wax blocks and their corresponding pathological sections in the sample library, traverse the unique identification codes of all abnormal samples in the abnormal area, and use the wax block and pathological section with the same unique identification code as a new sample and perform step S6 processing.

[0018] As an optimization of the technical solution of the present invention, step S5 further includes:

[0019] When the unique identification codes of all abnormal samples in the abnormal area are not the same, discard the pathological sections in the above abnormal samples, re-slice the wax blocks in the above abnormal samples for preparation, and execute step S2.

[0020] As an optimization of the technical solution of the present invention, step S5 further includes:

[0021] When there are two or more wax blocks with the same unique identification code in the identification results, discard the corresponding wax blocks and pathological sections.

[0022] As an optimization of the technical solution of the present invention, in step S7, comparing the pathological section scanning result with the wax block scanning result includes:

[0023] Comparing the shapes of the pathological section scanning result and the wax block scanning result.

[0024] As an optimization of the technical solution of the present invention, evaluating the pathological section to be inspected based on the comparison result specifically includes:

[0025] When the shape of the section scanning result is different from that of the wax block scanning result, discard the pathological section to be inspected and prepare a new section;

[0026] When the pathological section to be inspected has incomplete sectioning, deduct 1 point of A from the pathological section to be inspected;

[0027] When the pathological section to be inspected has uneven thickness, deduct 2 points of A from the pathological section to be inspected;

[0028] When the pathological section to be inspected has knife marks, cracks, or vibration marks, deduct 3 points of A from the pathological section to be inspected;

[0029] When the pathological section to be inspected has wrinkles or folds, deduct 4 points of A from the pathological section to be inspected;

[0030] When the pathological section to be inspected has contaminants, deduct 5 points of A from the pathological section to be inspected;

[0031] When the pathological section to be inspected has air bubbles or glue overflow, deduct 6 points of A from the pathological section to be inspected;

[0032] When the pathological section to be inspected has poor transparency and unclear structure, deduct 7 points of A from the pathological section to be inspected;

[0033] When the pathological section to be inspected has loose sectioning or improper mounting, deduct 8 points of A from the pathological section to be inspected;

[0034] When the pathological section to be inspected is untidy and the number is unclear, deduct 9 points of A from the pathological section to be inspected;

[0035] When the pathological section to be inspected has any one of over-dark or over-light nuclear or cytoplasmic staining, deduct 10 points of A from the pathological section to be inspected;

[0036] When the pathological section to be inspected has mislabeled or misretrieved tissue sections, deduct11 Processing of scores;

[0037] Calculate the total score SUM, where A i ≥ 0, and when the corresponding score deduction processing is not performed on the pathological section to be quality inspected, when calculating the total score SUM, the corresponding value of A i is 0;

[0038] Compare the total score SUM with a preset evaluation form to obtain an evaluation result.

[0039] In a second aspect, the present invention also hopes to provide a pathological tissue section quality control device, including a preparation module, a sample library, and a quality inspection device;

[0040] The preparation module is configured to: obtain a wax block and a pathological section, and assign the same unique identification code to the wax block and its corresponding pathological section;

[0041] The sample library is configured to: store wax blocks and pathological sections with unique identification codes;

[0042] The quality inspection device is configured to: identify the unique identification codes of the pathological section to be quality inspected and the wax block in the selected sample library. When the identification results of the unique identification codes of the pathological section to be quality inspected and the wax block are different, define the pathological section to be quality inspected and the wax block as abnormal samples. When the identification results of the unique identification codes of the pathological section to be quality inspected and the wax block are the same, scan the pathological section to be quality inspected and the wax block respectively to obtain a pathological section scan result and a wax block scan result, and use AI to compare the pathological section scan result with the wax block scan result, and evaluate the pathological section to be quality inspected based on the comparison result.

[0043] As a preference of the technical solution of the present invention, comparing the pathological section scan result with the wax block scan result includes: comparing the shapes of the pathological section scan result and the wax block scan result.

[0044] As a preference of the technical solution of the present invention, evaluating the pathological section to be quality inspected based on the comparison result includes:

[0045] When the shape of the section scan result is different from the shape of the wax block scan result, discard the pathological section to be quality inspected and re-prepare the section;

[0046] When the pathological section to be quality inspected has incomplete sections, perform a processing of deducting A1 points on the pathological section to be quality inspected;

[0047] When the pathological section to be quality inspected has uneven thickness, perform a processing of deducting A2 points on the pathological section to be quality inspected;

[0048] When there are knife marks, cracks, or tremor marks on the pathological section to be quality inspected, a penalty of 3 points of A is imposed on the pathological section to be quality inspected;

[0049] When there are wrinkles or folds on the pathological section to be quality inspected, a penalty of 4 points of A is imposed on the pathological section to be quality inspected;

[0050] When there are contaminants on the pathological section to be quality inspected, a penalty of 5 points of A is imposed on the pathological section to be quality inspected;

[0051] When there are air bubbles or glue overflow on the pathological section to be quality inspected, a penalty of 6 points of A is imposed on the pathological section to be quality inspected;

[0052] When the pathological section to be quality inspected has poor transparency and unclear structure, a penalty of 7 points of A is imposed on the pathological section to be quality inspected;

[0053] When the pathological section to be quality inspected is loose or improperly mounted, a penalty of 8 points of A is imposed on the pathological section to be quality inspected;

[0054] When the pathological section to be quality inspected is not clean or the number is not clear, a penalty of 9 points of A is imposed on the pathological section to be quality inspected;

[0055] When any one of the nucleus or cytoplasm of the pathological section to be quality inspected is overstained or understained, a penalty of A 10 points is imposed on the pathological section to be quality inspected;

[0056] When the wrong tissue section is pasted or fished for the pathological section to be quality inspected, a penalty of A 11 points is imposed on the pathological section to be quality inspected;

[0057] Calculate the total score SUM, wherein, A i ≥0, and when the corresponding score deduction process is not performed on the pathological section to be quality inspected, when calculating the total score SUM, the value of the corresponding A i is 0;

[0058] Compare the total score SUM with the preset evaluation form to obtain the evaluation result.

[0059] Compared with the prior art, the present invention has the following beneficial effects:

[0060] The pathological tissue section quality control method and device provided by the present application ensure the correspondence between the wax block and the pathological section through the identification of the unique identification code. Then, through the comparison between the section scanning result and the wax block scanning result and subsequent evaluation, the quality of the pathological section is controlled, realizing intelligent pathological section quality control and improving the efficiency and quality of pathological section quality control. Brief Description of the Drawings

[0061] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required in the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0062] Figure 1 It is a flowchart of the pathological tissue section quality control method provided by the embodiment of the present application;

[0063] Figure 2 It is a schematic diagram of a feasible deduction strategy provided by the embodiment of the present application;

[0064] Figure 3 It is a schematic diagram of the HE section quality evaluation obtained by adopting the technical solution of the present application;

[0065] Figure 4 It is a statistical table of the excellent rate of HE-stained sections obtained based on the technical solution of the present application. Detailed Embodiments

[0066] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in combination with the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, rather than all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0067] In this article, the term "include" is intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements not only includes those elements, but also includes other elements not explicitly listed, or elements inherent to this process, method, article or device. Without more limitations, the elements defined by the statement "include..." do not exclude the existence of additional identical elements in the process, method, article or device including the said elements.

[0068] Noun Explanation:

[0069] Wax block: The tissue sample sent by the patient. After a series of operations such as material collection and dehydration in the pathology department, a small tissue block will be made. This tissue block will be embedded in a plastic embedding cassette, and then liquid paraffin will be injected into the embedding cassette. After the liquid paraffin cools and solidifies, a tissue wax block is formed, that is, the wax block mentioned in the present invention.

[0070] White slide: The prepared wax block is cut into a wax film with a thickness of only 4 μm on a high-precision slicing machine. After floating the slice, the wax film is attached to a glass slide, which is the white slide.

[0071] Section: After the white slide is dewaxed at high temperature, it continues through the hematoxylin-eosin staining procedure (HE staining). The cell nuclei are stained purple-blue (the color of hematoxylin), and the cytoplasm is stained red (the color of eosin). Finally, it is covered with a coverslip, which is the (HE) section.

[0072] See Figure 1 As shown, this embodiment partially discloses a method for quality control of pathological tissue sections, including the following steps:

[0073] S1. Obtain wax blocks and pathological sections;

[0074] S2. Assign the same unique identification code to the wax block and its corresponding pathological section, and store them in the sample library;

[0075] S3. Select the pathological sections and wax blocks to be quality inspected in the sample library constructed in step S2;

[0076] S4. Identify the unique identification codes of the pathological sections and wax blocks to be quality inspected selected in step S3;

[0077] S5. When the identification results of the unique identification codes of the pathological sections and wax blocks to be quality inspected are the same, proceed to step S6; otherwise, define the pathological sections and wax blocks to be quality inspected as abnormal samples;

[0078] S6. Scan the pathological sections and wax blocks to be quality inspected respectively to obtain the pathological section scan results and wax block scan results;

[0079] S7. Use AI to compare the pathological section scan results with the wax block scan results, and evaluate the pathological sections to be quality inspected based on the comparison results.

[0080] In the above technical solution, improvements are made around the following two links to improve the quality control of pathological sections. First, the strict matching of the information of pathological sections and wax blocks is improved based on the unique identification code, ensuring that the information of pathological sections is error-free; second, the quality of pathological sections is controlled, and unqualified pathological sections are screened out, ensuring the quality of pathological sections and improving the efficiency of section analysis work. It should be emphasized that the process of obtaining wax blocks and pathological sections in step S1 is part of normal medical work and is obtained through the transfer of relevant hospital organizations or departments; the operation of assigning a unique identification code in step S2 can be understood as tagging, which is also a relatively common operation and can be understood as a general operation that those skilled in the art can master.

[0081] In some embodiments, the unique identification code mentioned in step S2 is used to store identity information and sample data. The identity information at least includes an identity serial number, and the sample data at least includes one or more of sample source, preparation time, and sample content. It should be noted that the unique identification code is assigned to the wax block and its corresponding pathological section when the wax block is obtained and the pathological section is prepared from the wax block. Its function is to uniformly represent and identify the wax block and the pathological section, so as to ensure that the wax block and the pathological section used in the quality inspection process are corresponding.

[0082] In some feasible embodiments, step S5 further includes: when the pathological section and the wax block to be quality inspected are defined as abnormal samples, storing the abnormal samples in the abnormal area of the sample library for storing all abnormal samples, and after completing the quality inspection of all wax blocks and their corresponding pathological sections in the sample library, traversing the unique identification codes corresponding to all abnormal samples in the abnormal area, and taking the wax block and the pathological section with the same unique identification code as a new sample and performing step S6 processing.

[0083] In the above technical solution, since the problem of misplacement may occur during the sample storage process, the sample library stores both the pathological sections and wax blocks that pass the quality inspection in step S5 and the pathological sections and wax blocks with quality inspection abnormalities. The samples passing the quality inspection and the samples with quality inspection abnormalities are separately set in the sample library. The samples passing the quality inspection can normally enter the subsequent scanning process. The samples with quality inspection abnormalities may be misjudged with a small probability due to human or external factors. Therefore, all abnormal samples stored in the sample library can be re-inspected and confirmed. If there are pathological sections and wax blocks with the same unique identification code after quality inspection confirmation, they can enter the subsequent scanning process as normal samples. By doing so, the integrity and rationality of the matching work of pathological sections and wax blocks are ensured to the greatest extent. In short, by taking the wax block and the pathological section with the same unique identification code as a new sample, the misplaced samples can be re-paired. On the one hand, the utilization rate of samples during quality inspection is improved, and on the other hand, the reliability of subsequent quality control results is ensured.

[0084] In some feasible embodiments, step S5 further includes: when the unique identification codes corresponding to all abnormal samples in the abnormal area are all different, discarding the pathological sections in the above abnormal samples, re-slicing and preparing the wax blocks in the above abnormal samples, and performing step S2.

[0085] In the above technical solution, during the sample storage process, situations such as missed placement may occur. Therefore, during the re-quality inspection of abnormal samples, there may still be a situation where the unique identification codes of the pathological sections and the wax blocks do not match. In this case, the pathological sections need to be discarded, and new sections need to be cut based on the wax blocks and assigned unique identification codes. Therefore, through this step, invalid pathological sections can be eliminated, and the correspondence between the wax blocks and the pathological sections can be ensured, thereby ensuring the reliability of subsequent quality control results.

[0086] In some feasible embodiments, step S5 further includes: when there are two or more wax blocks with the same unique identification code in the recognition result, the corresponding wax blocks and pathological sections are discarded.

[0087] In the above technical solution, since errors cannot be completely avoided during the assignment of unique identification codes, when such a situation of repeated code assignment occurs, through this step, the situation of confusion in the correspondence between the wax blocks and the pathological sections can be avoided, thereby improving the efficiency of the subsequent quality control process and ensuring the reliability of the subsequent quality control results.

[0088] Obviously, for different situations in the quality inspection and recognition link, different countermeasures are taken in the embodiments of the present invention, ensuring the scientificity and rationality of the recognition link, and thus ensuring the quality of section quality control.

[0089] In some feasible embodiments, the comparison of the pathological section scanning result and the wax block scanning result mentioned in step S7 includes: comparing the shapes of the pathological section scanning result and the wax block scanning result. In the above technical solution, wax block scanning refers to embedding a fixed tissue sample in a wax block and then using a scanner to perform digital scanning on the entire wax block to obtain a high-resolution image of the wax block; section scanning refers to using a high-resolution scanner to scan the entire section to obtain a digital image. The comparison mentioned includes: placing the wax block scanning image and the section scanning image together for comparative analysis, which can help observe the morphological changes of the tissue sample in the intact state and the section state; at the same time, by comparing the two scanning images, pathologists can better analyze the integrity of the tissue structure, cell morphology, tissue arrangement and other characteristics, providing important information for disease diagnosis. The above-mentioned scanning and comparison work can be generally understood with reference to the prior art. It should be emphasized that the comparison of the scanning results using AI technology mentioned above is prior art and can be carried out in any manner mentioned in the prior literature. The present invention does not describe or limit the specific implementation manner or process thereof, as long as those skilled in the art can implement it.

[0090] After the comparison is completed, evaluation is required, specifically including: when the shape of the section scanning result is different from the shape of the wax block scanning result, the pathological section to be quality inspected is discarded and re-made;

[0091] When the pathological section to be quality inspected is incomplete, the pathological section to be quality inspected is processed by deducting A1 point;

[0092] When the pathological section to be quality inspected has uneven thickness, the pathological section to be quality inspected is processed by deducting A2 points;

[0093] When the pathological section to be quality inspected has knife marks, cracks, or vibration marks, the pathological section to be quality inspected is processed by deducting A3 points;

[0094] When the pathological section to be quality inspected has wrinkles or folds, the pathological section to be quality inspected is processed by deducting A4 points;

[0095] When the pathological section to be quality inspected has contaminants, the pathological section to be quality inspected is processed by deducting A5 points;

[0096] When the pathological section to be quality inspected has air bubbles or glue overflow, the pathological section to be quality inspected is processed by deducting A6 points;

[0097] When the pathological section to be quality inspected has poor transparency and unclear structure, the pathological section to be quality inspected is processed by deducting A7 points;

[0098] When the pathological section to be quality inspected is loose or improperly mounted, the pathological section to be quality inspected is processed by deducting A8 points;

[0099] When the pathological section to be quality inspected is untidy and the number is unclear, the pathological section to be quality inspected is processed by deducting A9 points;

[0100] When the pathological section to be quality inspected has any one of over-dark or over-light nuclear or cytoplasmic staining, the pathological section to be quality inspected is processed by deducting A 10 points;

[0101] When the pathological section to be quality inspected has the wrong tissue section pasted or the wrong tissue section fished, the pathological section to be quality inspected is processed by deducting A 11 points;

[0102] Calculate the total score SUM, where, A i ≥0, and when the corresponding score deduction process is not performed on the pathological section to be quality inspected, when calculating the total score SUM, the value of the corresponding A i is 0;

[0103] Compare the total score SUM with the preset evaluation form to obtain the evaluation result.

[0104] It can be understood that the total score SUM is the sum of the corresponding deducted scores. Therefore, when the total score SUM is larger, it indicates that there are more "problems" in the pathological section, and the corresponding evaluation result is worse. By doing so, the evaluation process can be simplified. Moreover, the size of the problems in the pathological section can be assisted in reflecting through the setting of the size of the corresponding deducted scores, improving the efficiency of the evaluation.

[0105] It is feasible that, based on the technical solution described in this embodiment, a deduction strategy as Figure 2 shown can be set. And, in the specific implementation process of this embodiment, the preset evaluation form can be obtained in any form in the prior art. For example: a table formed by corresponding the total score value and the evaluation result through technical experience or big data. Such a table is conventional for those of ordinary skill in the art and can change with the actual needs and quality requirements of pathological research. Therefore, this text does not specifically disclose it here.

[0106] In summary, the pathological tissue section quality control method of this embodiment ensures the correspondence between the wax block and the pathological section through the identification of the unique identification code. Then, through the comparison between the section scanning result and the wax block scanning result and subsequent evaluation, the quality of the pathological section is controlled, realizing intelligent pathological section quality control. Combining Figure 3 and 4 as shown, it can be seen that the solution provided by the present invention improves the efficiency and quality of pathological section quality control.

[0107] Correspondingly, in some embodiments of the present invention, a pathological tissue section quality control device is also disclosed, which is applicable to the above-mentioned pathological tissue section quality control method; the device includes a preparation module, a sample library, and a quality inspection device;

[0108] The preparation module is configured to: obtain a wax block and a pathological section, and assign the same unique identification code to the wax block and its corresponding pathological section;

[0109] The sample library is configured to: store the wax block and the pathological section with unique identification codes;

[0110] The quality inspection device is configured to: identify the unique identification codes of the pathological section to be quality inspected and the wax block in the selected sample library. When the identification results of the unique identification codes of the pathological section to be quality inspected and the wax block are different, the pathological section to be quality inspected and the wax block are defined as abnormal samples. When the identification results of the unique identification codes of the pathological section to be quality inspected and the wax block are the same, the pathological section to be quality inspected and the wax block are respectively scanned to obtain the pathological section scanning result and the wax block scanning result, and the AI is used to compare the pathological section scanning result with the wax block scanning result, and the pathological section to be quality inspected is evaluated based on the comparison result.

[0111] An example of the comparison and evaluation function of the scanning results achieved by the quality inspection equipment is as follows:

[0112] Comparing the pathological section scanning result with the wax block scanning result includes: comparing the shapes of the pathological section scanning result and the wax block scanning result. After the comparison is completed, evaluating the pathological section to be quality inspected based on the comparison result, including:

[0113] When the shape of the section scanning result is different from that of the wax block scanning result, discard the pathological section to be quality inspected and prepare a new section;

[0114] When the pathological section to be quality inspected has incomplete sections, deduct 1 point of A1 from the pathological section to be quality inspected;

[0115] When the pathological section to be quality inspected has uneven thickness, deduct 1 point of A2 from the pathological section to be quality inspected;

[0116] When the pathological section to be quality inspected has knife marks, cracks, or tremor marks, deduct 1 point of A3 from the pathological section to be quality inspected;

[0117] When the pathological section to be quality inspected has wrinkles or folds, deduct 1 point of A4 from the pathological section to be quality inspected;

[0118] When the pathological section to be quality inspected has contaminants, deduct 1 point of A5 from the pathological section to be quality inspected;

[0119] When the pathological section to be quality inspected has air bubbles or glue overflow, deduct 1 point of A6 from the pathological section to be quality inspected;

[0120] When the pathological section to be quality inspected has poor transparency and unclear structure, deduct 1 point of A7 from the pathological section to be quality inspected;

[0121] When the pathological section to be quality inspected has loose sections or improper mounting, deduct 1 point of A8 from the pathological section to be quality inspected;

[0122] When the pathological section to be quality inspected has untidy sections or unclear numbers, deduct 1 point of A9 from the pathological section to be quality inspected;

[0123] When the pathological section to be quality inspected has any one of over-dark or over-light nuclear or cytoplasmic staining, deduct 1 point of A 10 from the pathological section to be quality inspected;

[0124] When the pathological section to be quality inspected has mislabeled or misfished tissue sections, deduct 1 point of A 11 from the pathological section to be quality inspected;

[0125] Calculate the total score SUM, where A i ≥0, and when the corresponding score deduction process is not performed on the pathological section to be quality inspected, when calculating the total score SUM, the corresponding value of A i is 0;

[0126] Compare the total score SUM with a preset evaluation form to obtain an evaluation result.

[0127] It should be noted that the pathological tissue section quality control device of this embodiment described above is applicable to the above-mentioned pathological tissue section quality control method. Therefore, the technical effects of the pathological tissue section quality control device disclosed in this embodiment are the same as those in the foregoing pathological tissue section quality control method, which will not be elaborated here. Moreover, for the technical means not disclosed in this pathological tissue section quality control device, the relevant descriptions in the foregoing pathological tissue section quality control method can be referred to correspondingly, which will not be elaborated here either.

[0128] In this embodiment, for software implementation, part or all of the processes of the embodiment can be completed by instructing relevant hardware through a computer program. When implemented, the above program can be stored in a computer-readable storage medium or transmitted as one or more instructions or codes on a computer-readable storage medium. Specifically, the computer-readable storage medium includes a computer storage medium and a communication medium, where the communication medium includes any medium facilitating the transmission of a computer program from one place to another. The storage medium can be any available medium that a computer can access. The computer-readable storage medium may include, but is not limited to, RAM, ROM, EEPROM, CD-ROM or other optical disc storage, magnetic disk storage medium or other magnetic storage device, or any other medium capable of carrying or storing the desired program code in the form of instructions or data structures and accessible by a computer.

[0129] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included in the protection scope of the present invention.

Claims

1. A method for quality control of pathological tissue sections, characterized in that: The steps include: S1. Obtain wax blocks and pathological sections; S2. Assign the same unique identification code to the wax block and its corresponding pathological section, and store them in the sample library; S3, selecting pathological sections and wax blocks to be inspected from the sample library constructed in step S2; S4, identifying the unique identification codes of the pathological sections and wax blocks to be inspected selected in step S3; S5. When the unique identification codes of the pathological sections and wax blocks to be inspected are the same, proceed to step S6; otherwise, the pathological sections and wax blocks to be inspected are defined as abnormal samples; S6. Scan the pathological sections and wax blocks to be inspected respectively to obtain scanning results of the pathological sections and the wax blocks; S7. Use AI to compare the pathological section scanning results with the wax block scanning results, and evaluate the pathological sections to be inspected based on the comparison results.

2. A pathological tissue section quality control method according to claim 1, characterized in that: In step S2, the unique identification code is used to store identity information and sample data, wherein the identity information includes at least an identity serial number, and the sample data includes at least one or more of sample source, preparation time, and sample content.

3. A pathological tissue section quality control method according to claim 1, characterized in that: Step S5 also includes: When the pathological sections and wax blocks to be inspected are defined as abnormal samples, the abnormal samples are stored in the abnormal area in the sample library for storing all abnormal samples. After the quality inspection of all wax blocks and their corresponding pathological sections in the sample library is completed, the unique identification codes corresponding to all abnormal samples in the abnormal area are traversed, and the wax blocks and pathological sections with the same unique identification codes are taken as new samples and processed in step S6.

4. A pathological tissue section quality control method according to claim 3, characterized in that: Step S5 also includes: When the unique identification codes corresponding to all abnormal samples in the abnormal area are different, the pathological sections in the abnormal samples are discarded, and the wax blocks in the abnormal samples are re-sliced ​​to prepare and execute step S2.

5. A pathological tissue section quality control method according to claim 3, characterized in that: Step S5 also includes: When there are two or more wax blocks with the same unique identification code in the recognition result, the corresponding wax blocks and pathological sections are discarded.

6. A pathological tissue section quality control method according to claim 1, characterized in that: In step S7, comparing the pathological section scanning result with the wax block scanning result includes: Compare the shapes of the pathology section scan results with those of the wax block scan results.

7. A pathological tissue section quality control method according to claim 6, characterized in that: The evaluation of the pathological sections to be inspected based on the comparison results specifically includes: When the shape of the slice scanning result is different from that of the wax block scanning result, the pathological slice to be inspected is discarded and a new slice is made; When the pathological section to be inspected is incomplete, the pathological section to be inspected will be deducted A1 points; When the pathological slice to be inspected is uneven in thickness, the pathological slice to be inspected is deducted A2 points; When there are knife marks, cracks, or chatter marks on the pathological slice to be inspected, A3 points will be deducted from the pathological slice to be inspected; When the pathological section to be inspected has wrinkles or folds, the pathological section to be inspected will be deducted A4 points; When there are contaminants in the pathological section to be inspected, the pathological section to be inspected will be deducted A5 points; When there are bubbles or glue overflow in the pathological section to be inspected, the pathological section to be inspected will be deducted A6 points; When the pathological section to be inspected has poor transparency and unclear structure, the pathological section to be inspected will be deducted A7 points; If the pathological sections to be inspected are loose or improperly mounted, A8 points will be deducted from the pathological sections to be inspected; If the pathological sections to be inspected are not neat and the numbers are not clear, the pathological sections to be inspected will be deducted A9 points; When the pathological section to be inspected has any one of the following: too dark or too light nuclear or cytoplasmic staining, the pathological section to be inspected is deducted A 10 Point processing; When the pathological slice to be inspected has a wrong tissue slice or a wrong tissue slice, the pathological slice to be inspected will be deducted A 11 Point processing; Calculate the total score SUM, Among them, A i ≥≥0, and when the pathological section to be inspected has not undergone the corresponding score deduction processing, when calculating the total score SUM, the corresponding A i The value of is 0; Compare the total score SUM with the preset evaluation table to obtain the evaluation result.

8. A pathological tissue section quality control device, characterized in that: Includes preparation modules, sample libraries and quality inspection equipment; The preparation module is configured to: obtain a wax block and a pathological section, and assign the same unique identification code to the wax block and its corresponding pathological section; The sample library is configured to: store wax blocks and pathological sections with unique identification codes; The quality inspection equipment is configured to: identify the unique identification codes of the pathological sections and wax blocks to be inspected in the selected sample library; when the identification results of the unique identification codes of the pathological sections and wax blocks to be inspected are different, define the pathological sections and wax blocks to be inspected as abnormal samples; when the identification results of the unique identification codes of the pathological sections and wax blocks to be inspected are the same, scan the pathological sections and wax blocks to be inspected respectively, obtain the pathological section scanning results and the wax block scanning results, use AI to compare the pathological section scanning results with the wax block scanning results, and evaluate the pathological sections to be inspected based on the comparison results.

9. A pathological tissue section quality control device according to claim 8, characterized in that: Comparing the pathological section scanning result with the wax block scanning result includes: comparing the shapes of the pathological section scanning result with the wax block scanning result.

10. A pathological tissue section quality control device according to claim 8, characterized in that: The evaluation of the pathological sections to be inspected based on the comparison results includes: When the shape of the slice scanning result is different from that of the wax block scanning result, the pathological slice to be inspected is discarded and a new slice is made; When the pathological section to be inspected is incomplete, the pathological section to be inspected will be deducted A1 points; When the pathological slice to be inspected is uneven in thickness, the pathological slice to be inspected is deducted A2 points; When there are knife marks, cracks, or chatter marks on the pathological slice to be inspected, A3 points will be deducted from the pathological slice to be inspected; When the pathological section to be inspected has wrinkles or folds, the pathological section to be inspected will be deducted A4 points; When there are contaminants in the pathological section to be inspected, the pathological section to be inspected will be deducted A5 points; When there are bubbles or glue overflow in the pathological section to be inspected, the pathological section to be inspected will be deducted A6 points; When the pathological section to be inspected has poor transparency and unclear structure, the pathological section to be inspected will be deducted A7 points; If the pathological sections to be inspected are loose or improperly mounted, A8 points will be deducted from the pathological sections to be inspected; If the pathological sections to be inspected are not neat and the numbers are not clear, the pathological sections to be inspected will be deducted A9 points; When the pathological section to be inspected has any one of the following: too dark or too light nuclear or cytoplasmic staining, the pathological section to be inspected is deducted A 10 Point processing; When the pathological slice to be inspected has a wrong tissue slice or a wrong tissue slice, the pathological slice to be inspected will be deducted A 11 Point processing; Calculate the total score SUM, Among them, A i ≥≥0, and when the pathological section to be inspected has not undergone the corresponding score deduction processing, when calculating the total score SUM, the corresponding A i The value of is 0; Compare the total score SUM with the preset evaluation table to obtain the evaluation result.

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