Sheet-like structure and method for using same

Abstract

The present invention pertains to a sheet-like structure that has a porous structure layer in which at least a part of a flow path having a porous structure through which a fluid can flow is exposed on the surface, and a support layer, and that is characterized in that the flow path has a separation part that separates adjacent flow paths and is capable of blocking the flow of the fluid in the flow path.

Description

Technical Field

[0001] The present invention relates to a sheet-like structure and a method of using the same. Background Art

[0002] In daily life and clinical settings, the development of inspection devices that enable simple and rapid diagnosis is underway. As an inspection device, a pregnancy test drug is cited as a representative example. If a test liquid containing a target substance such as an antigen is introduced into the inspection device, the test liquid flows through a flow path inside the inspection device. In this case, a labeling medium such as an antibody previously added to the flow path reacts with the target substance in the test liquid to develop color, and the presence of the target substance can be confirmed.

[0003] An inspection chip, which is an example of an inspection device, is sometimes referred to as "μ-PADs (microfluidic paper-based analytical devices)" and has a number of advantages such as (1) being inexpensive, (2) having no pump, (3) not requiring a large-scale device, and (4) being easy to discard, and research for improvement has been conducted worldwide.

[0004] Various substances have been reported as inspection chips (inspection devices). For example, for the purpose of sufficiently ensuring the reaction time between a phosphorus-based pesticide as a specimen and acetylcholine esterase (AChE) that reacts with the phosphorus-based pesticide, and providing a device with a stronger color development intensity and higher accuracy in the color reaction, an inspection device in which a liquid absorption pad (paper disk) is provided between layers of multiple paper chips forming the inspection device has been proposed (see Non-Patent Document 1). This inspection device utilizes the flow delay of the liquid generated by the liquid absorption pad to ensure the reaction time between the specimen and AChE. In addition, for the purpose of significantly suppressing color unevenness, an inspection chip in which a three-dimensional flow path is formed in a single sheet-like raw material has been proposed (see Patent Document 1).

[0005] Prior Art Documents

[0006] Patent Documents

[0007] Patent Document 1: Japanese Unexamined Patent Application Publication No. 2021-175970

[0008] Non-Patent Documents

[0009] Non-Patent Document 1: Quoc Trung Hua et al., analytical sciences, April, 2019, Vol. 35, p393-399 Summary of the Invention

[0010] Problems to be Solved by the Invention

[0011] However, in the inspection device including the technology described in the above Non-Patent Document 1, due to the flow of the liquid in which the primary reaction (the reaction between the specimen and AChE) is not sufficiently carried out being observed, and multiple base materials having flow paths formed thereon being bonded with double-sided tape, it is necessary to fill the gap in the thickness portion of the double-sided tape generated between the base materials, and there are concerns about complex assembly and processing. In addition, in the inspection chip described in the above Patent Document 1, the flow rate of the liquid is fast, and the reaction time in the primary reaction cannot be sufficiently ensured. Therefore, it is necessary to pre-mix the specimen and the inspection reagent (for example, antigen and antibody), and add them after the reaction. At this time, there is room for improvement in terms of the disposal of the container used for mixing and reaction, the contamination of the specimen, and simplicity, etc.

[0012] The subject of the present invention is to solve the above various problems in the past and achieve the following purposes. That is, the object of the present invention is to provide a sheet-like structure that can sufficiently ensure the reaction time and improve the accuracy of quantitative analysis.

[0013] Method for solving the problem

[0014] As a method for solving the above problem, it is as follows.

[0015] <1> A sheet-like structure, characterized by having:

[0016] A porous structure layer in which at least a part of the flow path of the porous structure capable of flowing a fluid is exposed on the surface, and a support layer,

[0017] The above flow path has a separation portion that separates adjacent above flow paths and can block the flow of the above fluid in the above flow path.

[0018] <2> The sheet-like structure according to the above <1>,

[0019] The above separation portion is a cut portion that cuts the above flow path in a direction crossing the flow direction of the above fluid in the above flow path.

[0020] <3> The sheet-like structure according to the above <1> or <2>,

[0021] At least a part of the above flow path exposed on the surface is a fluid receiving portion.

[0022] <4> The sheet-like structure according to the above <3>,

[0023] The above separation portion separates the above fluid receiving portion and the above flow path adjacent to the above fluid receiving portion, and can block the flow of the above fluid received by the above fluid receiving portion to the above flow path adjacent to the above fluid receiving portion.

[0024] <5> The sheet-like structure according to the above <3> or <4>,

[0025] The above support layer is disposed in a region of the above porous structure layer that does not lie in the above fluid receiving portion.

[0026] <6> The sheet-like structure according to any one of <1> to <5> above,

[0027] The above support layer is water-impermeable.

[0028] <7> The sheet-like structure according to any one of <1> to <6> above,

[0029] The exposed shape of the above flow path exposed on one surface in the above porous structure layer is different from the exposed shape of the above flow path exposed on the other surface.

[0030] <8> The sheet-like structure according to any one of <3> to <5> above,

[0031] At least a part of the above flow path exposed on the surface is a detection portion, and the detection portion is disposed at a position different from the above fluid receiving portion in the above flow path.

[0032] <9> A method of using a sheet-like structure, characterized in that,

[0033] It is a method of using the sheet-like structure according to any one of <1> to <8> above,

[0034] With the separation portion as a reference, the first flow path on one side receives the fluid, and the fluid received by the first flow path does not flow into the second flow path on the other side with the separation portion as a reference. In the separation portion, the first flow path and the second flow path are separated to block the flow of the fluid.

[0035] After a certain period of time has passed, in the separation portion, the first flow path and the second flow path are brought into contact, and the fluid is allowed to flow between the first flow path and the second flow path.

[0036] <10> The method of using a sheet-like structure according to <9> above,

[0037] The first flow path is a fluid receiving portion, and a detection portion is disposed in a part of the second flow path.

[0038] Effects of the Invention

[0039] According to the present invention, it is possible to provide a sheet-like structure that sufficiently ensures the reaction time and can improve the accuracy of quantitative analysis. Description of the Drawings

[0040] Figure 1ASchematic perspective view of the sheet-like structure of the first mode.

[0041] Figure 1B Is for Figure 1A Schematic cross-sectional view when the sheet-like structure is cut along the line a-a'.

[0042] Figure 2A Explanatory drawing for explaining the function of the separation part.

[0043] Figure 2B Explanatory drawing for explaining other functions of the separation part.

[0044] Figure 3A Explanatory drawing for explaining an example of the distribution of the support layer.

[0045] Figure 3B Explanatory drawing for explaining another example of the distribution of the support layer.

[0046] Figure 3C Explanatory drawing for explaining yet another example of the distribution of the support layer.

[0047] Figure 4A Schematic cross-sectional view of the sheet-like structure of the second mode.

[0048] Figure 4B Is for observing from the α direction Figure 4A Schematic plan view of the porous structure layer.

[0049] Figure 4C Is for observing from the β direction Figure 4A Schematic plan view of the porous structure layer.

[0050] Figure 5A Schematic cross-sectional view of the sheet-like structure of the third mode.

[0051] Figure 5B Is for observing from the α direction Figure 5A Schematic plan view of the porous structure layer.

[0052] Figure 5C Is for observing from the β direction Figure 5A Schematic plan view of the porous structure layer.

[0053] Figure 6A Schematic cross-sectional view of the sheet-like structure of the fourth mode.

[0054] Figure 6B Is for observing from the α direction Figure 6A Schematic plan view of the porous structure layer.

[0055] Figure 6C Is for observing from the β direction Figure 6ASchematic plan view of the porous structure layer

[0056] Figure 7A Schematic plan view of the porous structure layer in the sheet-like structure of the fifth mode as viewed from the α direction.

[0057] Figure 7B Schematic plan view of the porous structure layer in the sheet-like structure of the fifth mode as viewed from the β direction.

[0058] Figure 8A Schematic plan view of the porous structure layer in the sheet-like structure of the sixth mode as viewed from the α direction.

[0059] Figure 8B Schematic plan view of the porous structure layer in the sheet-like structure of the sixth mode as viewed from the β direction.

[0060] Figure 9A Schematic plan view of the porous structure layer in the sheet-like structure of the seventh mode as viewed from the α direction.

[0061] Figure 9B Schematic plan view of the porous structure layer in the sheet-like structure of the seventh mode as viewed from the β direction.

[0062] Figure 10A Schematic plan view of the porous structure layer in the sheet-like structure of the eighth mode as viewed from the α direction.

[0063] Figure 10B Schematic plan view of the porous structure layer in the sheet-like structure of the eighth mode as viewed from the β direction.

[0064] Figure 11A Schematic plan view of the porous structure layer in the sheet-like structure of the ninth mode as viewed from the α direction.

[0065] Figure 11B Schematic plan view of the porous structure layer in the sheet-like structure of the ninth mode as viewed from the β direction.

[0066] Figure 12A Schematic plan view of the porous structure layer in the sheet-like structure of the tenth mode as viewed from the α direction.

[0067] Figure 12B Schematic plan view of the porous structure layer in the sheet-like structure of the tenth mode as viewed from the β direction.

[0068] Figure 13A Explanatory drawing for explaining the specific flow path shape in the embodiment.

[0069] Figure 13BIt is an explanatory diagram for explaining a specific flow path shape in the embodiment.

[0070] Figure 13C It is an explanatory diagram for explaining a missing flow path shape in the embodiment.

[0071] Figure 13D It is an explanatory diagram for explaining a missing flow path shape in the embodiment.

[0072] Figure 14A It is an explanatory diagram for explaining a single-sided or double-sided printed flow path in the embodiment.

[0073] Figure 14B It is for the single-sided printed flow path Figure 14A The cross-sectional photograph when cut along the f-f' line.

[0074] Figure 14C It is for the double-sided printed flow path Figure 14A The cross-sectional photograph when cut along the f-f' line.

[0075] Figure 15 It is a schematic cross-sectional view for explaining the porous structure layer in the embodiment.

[0076] Figure 16 It is a schematic cross-sectional view for explaining the sheet-like structure in the embodiment.

[0077] Figure 17 It is a schematic cross-sectional view for explaining the sheet-like structure in the embodiment.

[0078] Figure 18A It is a graph showing the measurement results of quantitative analysis in the embodiment.

[0079] Figure 18B It is a graph showing the measurement results of quantitative analysis in the embodiment. Detailed Description of the Invention

[0080] (Sheet-like Structure)

[0081] The sheet-like structure of the present invention is characterized in that it has a porous structure layer in which at least a part of the flow path capable of flowing a fluid is exposed on the surface, and a support layer,

[0082] The above flow path has a separation part that separates adjacent above flow paths and can block the flow of the above fluid in the above flow path.

[0083] Hereinafter, the present invention will be described in detail based on several embodiments, without being limited by any of the following descriptions.

[0084] <First Mode>

[0085] Figure 1ASchematic perspective view of the sheet-like structure of the first mode. Figure 1B For Figure 1A Schematic cross-sectional view when the sheet-like structure is cut along the line a-a'.

[0086] The sheet-like structure 11 has a porous structure layer 101, a support layer 301, and a separation part X. Details will be described later. The sheet-like structure 11 has such a configuration that it can block the flow of fluid in the flow path of the porous structure layer 101. For example, it can sufficiently ensure the reaction time between the specimen and the reaction reagent.

[0087] - Porous structure layer -

[0088] In the porous structure layer 101 of the sheet-like structure 11, non-flow paths Y are provided in the flow paths A, B, C formed by the porous structure through which fluid can flow due to capillary action, etc., and in the regions other than the flow paths A - C. The flow path C is connected to either of the flow paths A and B, and fluid can flow in the order of flow path A, flow path C, flow path B, or flow path B, flow path C, flow path A. For example, Figure 1B As shown, when the fluid is received by the flow path A, the fluid flows in the order of flow path A, flow path C, flow path B.

[0089] The flow path of the porous structure may be formed in such a way that at least a part of it is exposed on the surface of the porous structure layer, and it may be formed in such a way that it is exposed on the entire surface of the porous structure layer. In addition, in the above-mentioned porous structure layer, the porous structure (flow path) may be formed only in a part of the porous structure layer or may be formed throughout the whole.

[0090] In the above-mentioned porous structure layer, at least a part of the flow path exposed on the surface is preferably a fluid receiving part. For example, Figure 1B In the shown porous structure layer, the flow path A for introducing fluid can be set as the fluid receiving part. In addition, in the above-mentioned porous structure layer, at least a part of the flow path exposed on the surface is preferably a detection part, and the detection part is arranged at a position different from the fluid receiving part in the flow path. For example, Figure 1B In the shown porous structure layer, the flow path B can be set as the detection part.

[0091] Here, the "fluid" is not particularly limited as long as it can flow into the flow path of the porous structure due to capillary action, etc., and can be appropriately selected according to the purpose. When the sheet-like structure of the present invention is applied to an inspection device, for example, a solution containing a specimen and a reaction reagent that reacts with the specimen can be cited.

[0092] The viscosity of the fluid, if it is a viscosity at which the fluid can flow through the flow path of the porous structure, is not particularly limited and can be appropriately adjusted according to the purpose.

[0093] Here, the "porous structure" refers to a structure having a plurality of connected pores. Generally, it is sometimes referred to as a co-continuous structure or a monolithic structure. The pores that the porous structure can connect continuously expand three-dimensionally, and the fluid infiltrates (i.e., capillary action).

[0094] The cross-sectional shape of the pores in the porous structure can be appropriately set in consideration of physical properties such as the viscosity of the fluid. For example, a substantially circular shape, a substantially elliptical shape, a substantially polygonal shape, etc. can be cited. The size of the pores in the porous structure is not particularly limited and can be appropriately selected according to the purpose. The cross-sectional shape and size of the pores can be obtained from, for example, cross-sectional photographs taken by a scanning electron microscope (SEM) or the like.

[0095] The porosity in the porous structure can be appropriately set in consideration of physical properties such as the viscosity of the liquid. As a method for measuring the porosity, there is no particular limitation. For example, a method of filling unsaturated fatty acid (commercially available butter) in the porous structure, applying osmium staining, cutting out the internal cross-sectional structure using FIB, and using a scanning electron microscope (SEM) or the like to measure the porosity can be cited.

[0096] Regarding the distribution of the pores in the porous structure, if the fluid can flow through, it can be appropriately set in consideration of physical properties such as the viscosity of the fluid, and it is preferably uniformly distributed in the flow path region.

[0097] Regarding the shape of the flow paths A to C when viewed from above, if the fluid can flow through, there is no particular limitation and it can be appropriately selected according to the purpose. For example, a circular shape, an elliptical shape, a square shape, a rectangular shape, etc. can be cited.

[0098] Regarding the diameter of the flow paths A to B, there is no particular limitation and it can be appropriately selected according to the purpose. For example, it can be set to 3 mm or more and 10 mm or less. Regarding the flow path width of the flow path C, there is no particular limitation and it can be appropriately selected according to the purpose. For example, it can be set to 1 mm or more and 5 mm or less.

[0099] Regarding the material M of the flow paths A to C, if it has a porous structure through which the fluid can flow, there is no particular limitation and it can be appropriately selected according to the purpose. For example, paper such as filter paper, non-woven fabric, nitrocellulose, polypropylene, etc. can be cited. Among these, from the viewpoints of simplicity and low cost, filter paper is more preferred.

[0100] The non-flow path Y is the region other than the flow paths A to C in the porous structure layer, that is, the region where no fluid flow is found.

[0101] As the material M' for the non-flow path Y, if no fluid flow is detected, there is no particular limitation and it can be appropriately selected according to the purpose. For example, it can be obtained by impregnating the above-mentioned material M with a hydrophobic material. As this hydrophobic material, from the viewpoint of the ease of manufacturing the porous structure layer, the melting point is preferably 90 °C or lower. For example, wax or a wax-containing composition can be cited. This hydrophobic material can be appropriately blended with viscosity-adjusting components such as resins, dispersion aids, fillers, etc.

[0102] When the above-mentioned flow path is impregnated with a hydrophobic material for the material M, the hydrophobic material is preferably heated to melt it. The heating temperature at this time can be appropriately set in consideration of the melting points of the hydrophobic material and the viscosity-adjusting component.

[0103] The viscosity at the time of melting of the hydrophobic material can be appropriately set in a manner that can be impregnated into the porous structure layer, considering the average thickness, basis weight (density), etc. of the porous structure layer as desired.

[0104] When the above-mentioned flow path is impregnated with a hydrophobic material for the material M, the impregnation rate of the hydrophobic material with respect to the material M is preferably in the range of 14% or more and 32% or less.

[0105] By manufacturing the porous structure layer in such a way that the impregnation rate becomes 14% or more, the flow path wall surface (the interface between the material M and the material M') becomes sufficiently uniform. For example, the fluid flow from the flow path to the flow path can be made smoother. In addition, by manufacturing the porous structure layer in such a way that the impregnation rate becomes 32% or less, it is possible to sufficiently avoid adverse conditions such as blockage when the hydrophobic material is impregnated into the material M, and more surely obtain a porous structure layer having a desired flow path structure.

[0106] Here, the "impregnation rate" refers to the impregnation rate related to the material M' in the region formed by the material M' throughout the entire thickness direction in the porous structure layer. In addition, the above impregnation rate can be regarded as 100% for the material M' obtained by impregnating the material M in a hydrophobic material heated in such a way as to have a sufficiently low viscosity (for example, heated to 120 °C) and leaving it at the temperature for a sufficient time (for example, 3 minutes). The adjustment of the above impregnation rate can be carried out, for example, by adjusting the amount of the hydrophobic material impregnated (the thickness of the hydrophobic film, etc.).

[0107] As a method for measuring the impregnation rate of the hydrophobic material with respect to the material M, there is no particular limitation and it can be appropriately selected according to the purpose. For example, the following methods can be cited.

[0108] [Method for measuring impregnation rate]

[0109] Cut the filter paper into an appropriate size. After drying at 120 °C for 3 minutes, measure the dry mass M0 (g). Then, immerse the filter paper in a hydrophobic material and leave it at 120 °C for 3 minutes. Clamp the impregnated filter paper with the same type of filter paper and a glass slide, and leave it at 120 °C for 1 minute under a load of 100 gf to remove the excess hydrophobic material. Then, measure the mass M1 (g) of the filter paper, and calculate the maximum impregnation amount Pmax (g / m 2 ) according to the following formula.

[0110] Formula ··· Pmax (g / m 2 ) = (M1 - M0) × 1000

[0111] There is no particular limitation on the viscosity of the above-mentioned hydrophobic material, and it can be appropriately selected according to the purpose. For example, from the viewpoint of sufficiently avoiding adverse conditions such as clogging during impregnation of material M, at 140 °C and a shear rate of 3000 s -1 , the viscosity is preferably 100 mPa·s or less, more preferably 50 mPa·s or less, and further preferably 30 mPa·s or less. There is no particular limitation on the measurement of this viscosity. For example, a rheometer (e.g., trade name: AR-G2 rheometer, manufactured by TA instrument) can be used for measurement.

[0112] In order to make the flow of the liquid easily visible, material M' is preferably colored, and can be white or transparent, or can be uncolored. The coloring of material M' can be achieved, for example, by impregnating a coloring agent into material M in addition to the hydrophobic material. As such a coloring agent, for example, pigments represented by carbon black (black pigment) can be cited, and hydrophobic ones are preferred. In addition, as this coloring agent, a coloring agent that does not cause adverse effects on the reagents used for inspections and the like is preferably selected.

[0113] The shape of the above-mentioned porous structure layer 101 in plan view is not particularly limited and can be appropriately selected according to the purpose. For example, a rectangle, a substantially circular shape, a substantially elliptical shape, a substantially rectangular shape, etc. can be cited.

[0114] - Separation part -

[0115] In the porous structure layer 101 of the above-mentioned sheet-like structure 11, in the flow paths in the porous structure layer 101, a separation part is provided to separate adjacent flow paths and block the flow of the fluid in the flow paths. In addition, the above-mentioned separation part preferably separates the above-mentioned fluid receiving part and the above-mentioned flow path adjacent to the above-mentioned fluid receiving part, and can block the flow of the above-mentioned fluid received by the above-mentioned fluid receiving part to the above-mentioned flow path adjacent to the above-mentioned fluid receiving part. More specifically, for example, when Figure 1A and Figure 1BWhen the flow path A in the [description] is a fluid receiving part, the above-mentioned separation part preferably separates the flow path A and the flow path C adjacent to the flow path A, and can block the flow of the above-mentioned fluid received by the flow path A onto the flow path C.

[0116] Here, "separating adjacent flow paths to block the flow of fluid in the flow path" means that the porous structure forming the flow path is separated with the separation part as a reference, so that the state where the flow of fluid is not manifested is achieved.

[0117] The position where the above-mentioned separation part is arranged is not particularly limited as long as it can separate adjacent flow paths, and it is preferably between the above-mentioned fluid receiving part and the above-mentioned detection part, and in the area excluding the fluid receiving part and the detection part.

[0118] In addition, the number of the above-mentioned separation parts is not particularly limited and can be appropriately set according to the purpose. By increasing the number of the separation parts, multiple reactions (multi-stage reactions) can be carried out within a single sheet-like structure.

[0119] As the above-mentioned separation part, as long as it can separate adjacent flow paths, there is no particular limitation, and it can be appropriately selected according to the purpose, and a cutting part is preferred. The cutting part is as Figure 1A and Figure 1B shown, and is an area that cuts the flow path in a direction crossing the flow direction of the fluid in the above-mentioned flow path. Here, the "direction crossing the flow direction of the fluid" is not particularly limited. For example, Figure 1A as shown, it can be a right angle direction with respect to the flow direction of the fluid, or can be a substantially right angle direction.

[0120] In addition, as a method where the above-mentioned separation part is not a cutting part, for example, the porous structure can be stretched in the up-down direction or the left-right direction ( Figure 1A the left-right direction in [description]), and the area where the porous structures in the porous structure layer are separated from each other is set as the separation part. At this time, the support body described later is preferably a material (such as an elastic member) that can be stretched in the up-down direction or the left-right direction.

[0121] When the above-mentioned separation part is a cutting part, it is preferably that the cutting part only exists in the porous structure layer in the sheet-like structure, and does not exist in the support body layer described later. In other words, it is preferably arranged in such a way that when the sheet-like structure is separated with the cutting part as a reference, the porous structure layer is separated and the support body layer is not separated. The support body layer can be in a state of being directly connected without separation, so that the support body layer functions as a hinge to make the separated flow paths contact again and continue the flow of fluid.

[0122] Here, Figure 2A and Figure 2B are used to specifically illustrate the function of the above-mentioned separation part.

[0123] Figure 2A This is an explanatory diagram for an example of the function of the separation part. Specifically, it shows a diagram of the state where the sheet-like structure 11 shown is bent by about 45° with the separation part X as a reference. Figure 1A The figure showing the state where the sheet-like structure 11 shown is bent by about 45° with the separation part X as a reference. Figure 2B This is another explanatory diagram for an example of the function of the separation part. Specifically, it shows a diagram of the state where the sheet-like structure 11 shown is bent by about 180° with the separation part X as a reference. Additionally, as described above, Figure 1A The figure showing the state where the sheet-like structure 11 shown is bent by about 180° with the separation part X as a reference. Also, as described above, Figure 2A and Figure 2B In either state of

[0124] Even in either state of Figure 2A and Figure 2B the porous structure in the porous structure layer 101 is separated, that is, adjacent flow paths are separated, and the flow of the fluid in this flow path is blocked. More specifically, for example, when the flow path A is a fluid receiving part, through this separation part, the flow path A and the flow path C adjacent to this flow path A are separated, and the flow of the above-mentioned fluid received by this flow path A to this flow path C is blocked.

[0125] By having such a configuration, the above-mentioned sheet-like structure 11 can prevent the flow of the fluid in the flow path and allow the fluid to sufficiently stay in the fluid receiving part. In addition, as described above, the support layer 301 is not separated by the separation part and functions as a hinge. Therefore, after allowing the fluid to stay for a predetermined time, it is possible to, as Figure 1B shown, make the separated flow paths contact again and continue the flow of the fluid.

[0126] For example, when using this sheet-like structure 11 as an inspection device, it is possible to ensure sufficient reaction time between the specimen and the reagent (such as antigen and antibody, etc.) that reacts with the specimen by introducing the specimen and the reagent into the fluid receiving part of the sheet-like structure in the state of Figure 2A or Figure 2B . In addition, after the reaction is completed (or after a predetermined time has passed), it is possible to, as Figure 1B shown, make the separated flow paths contact again and continue the flow of the specimen and the reagent.

[0127] In addition, by having such a configuration, the above-mentioned sheet-like structure 11 can rectify the fluid received by the fluid receiving part. Specifically, it is possible to separate the flow path once through the separation part, thereby preventing the fluid from being biased in the flow path and the fluid receiving part due to the degree of fluid reception.

[0128] As the angle at which the above-mentioned sheet-like structure is bent with the separation part X as a reference, if it is an angle at which the flow path is separated and the flow of fluid is blocked, there is no particular limitation, and it can be appropriately set according to the purpose.

[0129] -Support layer-

[0130] The above-mentioned sheet-like structure 11 has a support layer 301.

[0131] As described above, the support layer 301 can function as a hinge of the sheet-like structure 11 to bring the separated flow paths into contact again and continue the flow of fluid. In addition, the support layer has the function of enhancing the physical strength of the sheet-like structure itself.

[0132] Here, use Figures 3A - 3C to specifically illustrate the distribution of the above-mentioned support layer. Figure 3A It is an explanatory diagram for an example of explaining the distribution of the support layer, Figure 3B It is an explanatory diagram for another example of explaining the distribution of the support layer, Figure 3C It is an explanatory diagram for still another example of explaining the distribution of the support layer.

[0133] The support layer in the above-mentioned sheet-like structure can be disposed in the region of the above-mentioned porous structure layer that is not located in the fluid receiving portion. For example, Figure 1A and Figure 1B as shown, it can be disposed only on the side of the surface where fluid is not introduced into the fluid receiving portion, Figure 3A as shown, it can be disposed on both surfaces of the porous structure layer. Further, the support layer is Figure 3B and Figure 3C as shown, it can be disposed so as to cover the exposed flow path or a part of the flow path. In addition, when the support layer is disposed on both surfaces of the porous structure layer, particularly on the side of the surface where fluid is introduced into the fluid receiving portion, it is preferably disposed so as not to overlap with the above-mentioned separation part.

[0134] The above-mentioned sheet-like structure has such a configuration that it can prevent contamination of the fluid in the flow path. For example, when the fluid is a specimen and a reagent (such as an antigen and an antibody, etc.), it can prevent reaction hindrance caused by contamination, and is suitable.

[0135] The above-mentioned support layer is preferably water-impermeable. As the material of the water-impermeable support layer, there is no particular limitation, and it can be appropriately set according to the purpose. For example, polypropylene, etc. can be cited.

[0136] As the size, structure and shape of the above-mentioned support layer, if it can cover the flow path in the porous structure layer, there is no particular limitation, and it can be appropriately set according to the purpose.

[0137] The above-mentioned support layer can use commercially available products. As such commercially available products, for example, those with the trade name 660-PF (manufactured by Nichiban Co., Ltd.) etc. can be cited.

[0138] There is no particular limitation on the average thickness of the sheet-like structure 11 of the first mode, and it can be appropriately selected according to the purpose. For example, it can be set to be 100 μm or more and 300 μm or less. This average thickness can be measured using a thickness gauge with the trade name id-c112bs (manufactured by Mitutoyo) etc.

[0139] There is no particular limitation on the size of the sheet-like structure 11 of the first mode, and it can be appropriately selected according to the purpose.

[0140] <Second mode>

[0141] The above-mentioned porous structure layer can be such that the exposed shape of the above-mentioned flow path exposed on one surface in the porous structure layer is different from the exposed shape of the above-mentioned flow path exposed on the other surface. In other words, the above-mentioned porous structure layer can have two or more layers with different flow path shapes.

[0142] Here, regarding the sheet-like structure 12 of the second mode, use Figures 4A - 4C , and specifically explain. Figure 4A is a schematic cross-sectional view of the sheet-like structure of the second mode, Figure 4B is a schematic plan view when observing the Figure 4A porous structure layer from the α direction, Figure 4C is a schematic plan view when observing the Figure 4A porous structure layer (that is, the first porous structure layer 102 and the second porous structure layer 202 after removing the support layer 301 from the sheet-like structure 12 of the second mode) from the β direction. In addition, Figure 4A is when cutting with the Figure 4B and Figure 4C indicated b-b' line, it is a schematic cross-sectional view. Further, Figure 4B and Figure 4C omit the illustration of the separation part X.

[0143] In the first porous structure layer 102 of the sheet-like structure 12 of the second mode, non-flow paths Y are provided in the flow path A, the flow path B, and the region other than the flow path A and the flow path B. The flow path A and the flow path B are isolated in the first porous structure layer 102.

[0144] In the second porous structure layer 202 of the sheet-like structure 12 of the second mode, non-flow paths Y are provided in the flow paths C, D, and E, and in regions other than the flow paths C, D, and E. The flow path E is connected to both the flow path C and the flow path D, and a fluid can flow in the order of the flow path C, the flow path E, and the flow path D, or in the order of the flow path D, the flow path E, and the flow path C.

[0145] In the sheet-like structure 12 of the second mode, a support layer 301 is provided on the surface on the side of the second porous structure layer 202.

[0146] In addition, there is no spacer between the first porous structure layer 102 and the second porous structure layer 202, and they are adjacent to each other.

[0147] In the sheet-like structure 12 of the second mode, the flow path A of the first porous structure layer 102 is adjacent to the flow path C of the second porous structure layer 202, and in addition, the flow path B of the first porous structure layer 102 is adjacent to the flow path D of the second porous structure layer 202. That is, in the sheet-like structure 12 of the second mode, they are adjacent and connected in the order of the flow path A, the flow path C, the flow path E, the flow path D, and the flow path B, or in the order of the flow path B, the flow path D, the flow path E, the flow path C, and the flow path A.

[0148] In the sheet-like structure 12 of the second mode, a separation part X is formed so as to block the flow path C and the flow path E.

[0149] In addition, Figure 4A When impregnating the hydrophobic material from the first porous structure layer 102, the front end region is depicted as a dotted line as an imaginary line. Similarly, when impregnating the hydrophobic material from the side of the second porous structure layer 202, the front end region is depicted as a dotted line as an imaginary line. This imaginary line is also described in the same way in other drawings.

[0150] Moreover, the flow paths A, B, C, D, E, and the non-flow path Y are formed of the material M or the material M' described in the items of the above <First Mode>.

[0151] Figure 4A As shown, in the sheet-like structure 12 of the second mode, they are adjacent in the order of the flow path A, the flow path C, the flow path E, the flow path D, and the flow path B, or in the order of the flow path B, the flow path D, the flow path E, the flow path C, and the flow path A, and are connected. In other words, in the sheet-like structure 12 of the second mode, for example, when a fluid is dropped onto the flow path A, the fluid passes through capillary action or the like, and is configured to flow through the flow path A, the flow path C, the flow path E, and the flow path D in this order, and finally flow through the flow path B. In this case, the flow path A can be set as a fluid receiving part, and the flow path B can be set as a detection part.

[0152] In the above-mentioned sheet-like structure, the ratio (t2 / t1) of the average thickness (t2) of the second porous structure layer to the average thickness (t1) of the first porous structure layer is preferably 0.56 or more and 2.2 or less. By manufacturing the sheet-like structure such that the ratio of the average thickness (t2 / t1) is 0.56 or more and 2.2 or less, it is possible to sufficiently avoid defective conditions such as blockage when the hydrophobic material is impregnated into the material M. For example, the flow velocity and / or velocity stability of the liquid from the flow path to the flow path can be effectively improved. Considering the same view, the ratio of the average thickness (t2 / t1) exceeds 1.0, that is, the average thickness (t2) of the second porous structure layer is more preferably larger than the average thickness (t1) of the first porous structure layer, further preferably 1.3 or more, and particularly preferably 1.8 or more. In addition, the ratio of the average thickness (t2 / t1) is not particularly limited and can be set to 3.0 or less.

[0153] The average thickness of the sheet-like structure 12 of the second method is not particularly limited and can be appropriately selected according to the purpose. For example, it can be set to 100 μm or more and 300 μm or less. This average thickness can be measured using a thickness gauge with the trade name id-c112bs (manufactured by Mitutoyo) or the like.

[0154] Regarding the size of the sheet-like structure 12 of the second method, there is no particular limitation and it can be appropriately selected according to the purpose.

[0155] <Third Method>

[0156] Regarding the sheet-like structure 13 of the third method, use Figures 5A - 5C to specifically describe. Figure 5A is a schematic cross-sectional view of the sheet-like structure of the third method, Figure 5B is a schematic plan view when observing the porous structure layer from the α direction Figure 5A and Figure 5C is a schematic plan view when observing the porous structure layer from the β direction Figure 5A . In addition, Figures 5A - 5C In the sheet-like structure 13 shown, the flow path structure of the sheet-like structure is different from that of the sheet-like structure of the second method. Other than this, it is the same as the sheet-like structure 12 of the second method. In addition, Figure 5A is a schematic cross-sectional view when cutting along the c-c' line shown by Figure 5B and Figure 5C . Further, Figure 5B and Figure 5C omit the illustration of the separation part X.

[0157] Figure 5B ​​​​​​​As shown, in the sheet-like structure 13 of the third mode, in the first porous structure layer 103, there are provided a flow path A, a flow path B, a flow path F connected to the flow path A, and a non-flow path Y which is a part other than the flow path F. The flow path A and the flow path B are isolated in the first porous structure layer 103 in the sheet-like structure 13 of the third mode.

[0158] Figure 5C As shown, in the second porous structure layer 203 of the sheet-like structure 13 of the third mode, a non-flow path Y is provided as a part other than the flow path D, the flow path E, the flow path D and the flow path E, and the flow path E is connected to the flow path D.

[0159] In the sheet-like structure 13 of the third mode, a support layer 301 is provided on the surface on the side of the second porous structure layer 203.

[0160] In the sheet-like structure 13 of the third mode, the flow path B in the first porous structure layer 103 is adjacent to the flow path D in the second porous structure layer 203, and the flow path F in the first porous structure layer 103 is connected to the flow path E in the second porous structure layer 203. That is, in the sheet-like structure 13 of the third mode, they are adjacent and connected in the order of the flow path A, the flow path F, the flow path E, the flow path D, the flow path B, or in the order of the flow path B, the flow path D, the flow path E, the flow path F, the flow path A.

[0161] In the sheet-like structure 13 of the third mode, a separation part X is formed in such a way as to block the flow path F and the flow path E.

[0162] Moreover, the flow path A, the flow path B, the flow path D, the flow path E, the flow path F, and the non-flow path Y are formed of the material M or the material M' described in the item of the <first mode>.

[0163] Figure 5A As shown, in the sheet-like structure 13 of the third mode, for example, when a fluid is dropped into the flow path A, the liquid is configured to flow through the flow path A, the flow path F, the flow path E, the flow path D in this order by capillary action or the like, and finally flow into the flow path B. In this case, the flow path A can be set as a fluid receiving part, and the flow path B can be set as a detection part.

[0164] Except for the above, the description of the matters common to the <first mode> is omitted.

[0165] <Fourth mode>

[0166] Regarding the sheet-like structure 14 of the fourth mode, use Figures 6A - 6C , and specifically explain. Figure 6A is a schematic cross-sectional view of the sheet-like structure of the fourth mode, Figure 6B is from the α direction Figure 6A a schematic plan view when observing the porous structure layerFigure 6C A schematic plan view when observing the porous structure layer from the β direction. In addition, Figure 6A In the sheet-like structure 14 shown, the flow path structure of the sheet-like structure is different from that of the sheet-like structure of the second mode. Other than that, it is the same as the sheet-like structure 12 of the second mode. In addition, Figures 6A - 6C A schematic cross-sectional view when cutting along the d-d' line shown by Figure 6A and Figure 6B and Figure 6C .

[0167] Figure 6B As shown, in the sheet-like structure 14 of the fourth mode, in the first porous structure layer 104, as flow path B, and in the part other than flow path B, a non-flow path Y is provided, and flow path A is not provided.

[0168] Figure 6C As shown, in the sheet-like structure 14 of the fourth mode, in the second porous structure layer 204, as flow paths A, D, E, A, E, and in the part other than flow paths D and A, a non-flow path Y is provided. This flow path E is connected to either of this flow path D and this flow path A.

[0169] In the sheet-like structure 14 of the fourth mode, a support layer 301 is provided on the surface on the side of the second porous structure layer 204.

[0170] In the sheet-like structure 14 of the fourth mode, flow path D of the first porous structure layer 104 is adjacent to flow path B of the second porous structure layer 204. That is, in the sheet-like structure 14 of the fourth mode, they are adjacent and connected in the order of flow path A, flow path E, flow path D, flow path B, or in the order of flow path B, flow path D, flow path E, flow path A.

[0171] In the sheet-like structure 14 of the fourth mode, a separation part X is formed in such a way as to block this flow path A and this flow path E.

[0172] Moreover, flow path A, flow path B, flow path D, flow path E, and non-flow path Y are formed of the material M or material M' described in the item of the above <First Mode>.

[0173] Figure 6A As shown, in the sheet-like structure 14 of the fourth mode, for example, when a fluid is dropped into flow path A, the liquid constitutes a mode of flowing through flow path A, flow path E, and flow path D in this order and finally flowing into flow path B through capillary action or the like. In this case, this flow path A can be set as a fluid receiving part, and flow path B can be set as a detection part.

[0174] Except for the above, for matters common to <First Mode>, the description is omitted.

[0175] <Fifth Mode>

[0176] Regarding the sheet-like structure of the fifth mode, use Figures 7A - 7B , and specifically describe it. Figure 7A FIG. is a schematic plan view of the porous structure layer in the sheet-like structure of the fifth mode when viewed from the α direction, Figure 7B FIG. is a schematic plan view of the porous structure layer in the sheet-like structure of the fifth mode when viewed from the β direction.

[0177] Figure 7A and Figure 7B As shown in Figure 4B and Figure 4C the same. In the sheet-like structure of the fifth mode, the flow path D provided in the second porous structure layer 205 has an annular structure and has a structure in which a non-flow path Y1 is formed inside it. In addition,

[0178] As the annular flow path D, it can have any contour shape such as a circle, an ellipse, or a rectangle. When the sheet-like structure is viewed from above, it preferably has a contour shape substantially consistent with that of the flow path B. In addition, the non-flow path Y formed inside the flow path D preferably has a shape that reduces the contour shape of the flow path D when the sheet-like structure is viewed from above.

[0179] <Sixth mode>

[0180] Regarding the sheet-like structure of the sixth mode, use Figures 8A - 8B , and specifically describe it. Figure 8A FIG. is a schematic plan view of the porous structure layer in the sheet-like structure of the sixth mode when viewed from the α direction, Figure 8B FIG. is a schematic plan view of the porous structure layer in the sheet-like structure of the sixth mode when viewed from the β direction.

[0181] Figure 8A and Figure 8B As shown in Figure 8B the second porous structure layer 206 of the sheet-like structure of the sixth mode has a structure in which multiple flow paths E ( Figure 7A in Figure 7B two flow paths E1 and E2) are provided. In addition,

[0182] <Seventh mode>

[0183] Regarding the sheet-like structure of the seventh mode, use Figures 9A - 9B , and specifically describe it. Figure 9A FIG. is a schematic plan view of the porous structure layer in the sheet-like structure of the seventh mode when viewed from the α direction, Figure 9BIt is a schematic plan view when observing the porous structure layer in the sheet-like structure of the seventh mode from the β direction.

[0184] Figure 9A and Figure 9B As shown, in the sheet-like structure of the seventh mode, there are combined Figure 7A , Figure 7B , Figure 8A , and Figure 8B the shown flow path shapes.

[0185] <Eighth Mode>

[0186] Regarding the sheet-like structure of the eighth mode, use Figures 10A - 10B to specifically explain. Figure 10A It is a schematic plan view when observing the porous structure layer in the sheet-like structure of the eighth mode from the α direction, Figure 10B and it is a schematic plan view when observing the porous structure layer in the sheet-like structure of the eighth mode from the β direction.

[0187] Figure 10A and Figure 10B As shown, in the sheet-like structure of the eighth mode, the second porous structure layer 208 has a structure with E3 flow paths (E3 in addition to E1 and E2), and other than that, it is substantially the same as Figure 9B . At this time, Figure 10B in the shown sheet-like structure, the way of opposing the three E flow paths to each other is connected to the D flow path.

[0188] <Ninth Mode>

[0189] Regarding the sheet-like structure of the ninth mode, use Figures 11A - 11B to specifically explain. Figure 11A It is a schematic plan view when observing the porous structure layer in the sheet-like structure of the ninth mode from the α direction, Figure 11B and it is a schematic plan view when observing the porous structure layer in the sheet-like structure of the ninth mode from the β direction.

[0190] Figure 11A and Figure 11B As shown, in the sheet-like structure of the ninth mode, the E3 flow path has a structure that branches into two (E31 and E32) and is connected to the D flow path, and other than that, it is substantially the same as Figure 10B .

[0191] <Tenth Mode>

[0192] Regarding the sheet-like structure of the tenth mode, use Figures 12A - 12B to specifically explain. Figure 12A It is a schematic plan view when observing the porous structure layer in the sheet-like structure of the tenth mode from the α direction, Figure 12BIt is a schematic plan view when observing the porous structure layer in the sheet-like structure of the 10th mode from the β direction.

[0193] Figure 12A and Figure 12B As shown, in the sheet-like structure of the 10th mode, in addition to the flow paths E1 and E2, it has a structure with the roots of the flow path E2 (E3 and E4). Other than this, it is substantially the same as Figure 11B At this time, Figure 12B In the sheet-like structure shown, the 4 flow paths E are opposed to each other and connected to the flow path D.

[0194] Figures 8A - 12B In the sheet-like structure shown, the number of the flow paths E (and the flow path F) is preferably 4 or less, more preferably 3 or less, and further preferably 2, from the viewpoint of suppressing the increase in the liquid amount. In addition, the number of connections between the flow path D and the flow path E is preferably 4 or less, more preferably 3 or less, and further preferably 2.

[0195] Preferably, at least 2 of the multiple flow paths E are opposed to each other and connected to the above-mentioned flow path D. In addition, at least 2 of the multiple flow paths E preferably have substantially the same shape.

[0196] For example, the above-mentioned sheet-like structure can be manufactured by forming a predetermined part (such as the flow path B) on a sheet-like raw material to produce the first porous structure layer, and at the same time forming a predetermined part (such as the flow path D) on another sheet-like raw material to produce the second porous structure layer, and then laminating them. Or, the above-mentioned sheet-like structure can be manufactured by forming a predetermined part on a part of a single sheet-like raw material to produce the first porous structure layer, and at the same time forming a predetermined part on another part of the single sheet-like raw material to produce the second porous structure layer, and then folding the single sheet-like raw material while adjusting the positions of the first porous structure layer and the second porous structure layer.

[0197] The sheet-like structure in the present invention is preferably manufactured by forming the first porous structure layer on one side of a single sheet-like raw material and forming the second porous structure layer on the other side. Such a sheet-like structure (1) formed by respectively forming the first porous structure layer and the second porous structure layer on both sides of a single sheet-like raw material can avoid the procedures and costs of lamination (or folding), (2) the liquid can surely flow between the first porous structure layer and the second porous structure layer due to capillary action, and (3) no fixtures for holding the lamination (or folding) of the sheet-like raw materials are required, so it has various advantages such as being easy to discard.

[0198] As a specific manufacturing method of the sheet-like structure, for example, it can be manufactured by using the following method.

[0199] [An Example of a Method for Manufacturing a Sheet Structure]

[0200] First, a hydrophobic material, a colorant, and a resin are mixed, for example, at a temperature of 100 °C or higher and 140 °C or lower to prepare a WAX ink. The WAX ink is coated on a substrate such as a polyethylene terephthalate film to produce an ink ribbon. Next, a thermal transfer printer (for example, trade name: Respuri R412v-ex, manufactured by Sato Holdings Co., Ltd.) can be used to print a specific flow path shape on offset paper, forming a missing flow path pattern in the printed portion of the ink ribbon. After fixing the ink ribbon with the missing flow path pattern on both the front and back of the filter paper, a thermal laminating material (for example, trade name: GL535ML, manufactured by GBG Co., Ltd.) set at a predetermined temperature and linear velocity is used to transfer and penetrate the WAX ink into the filter paper, forming a three-dimensional flow path to produce a sheet structure.

[0201] Here, there are no particular restrictions on the filter paper, and it can be appropriately set according to the purpose. For example, a filter paper with an average thickness of 310 μm, a basis weight of 94 g / m 3 , and a CFR (Capillary flow rate) of 13.9 sec / 4 cm can be used.

[0202] Here, the "predetermined temperature and linear velocity" refers to the conditions that enable the penetration and transfer of the WAX ink with respect to the filter paper, and there are no particular restrictions. During transfer, it can be at 85 °C and a linear velocity of 10 mm / sec, and during penetration, it can be at 85 °C and a linear velocity of 5 mm / sec.

[0203] (Method of Using the Sheet Structure)

[0204] The method of using the sheet structure of the present invention is characterized in that, with the separation portion as a reference, a first flow path on one side receives a fluid, and the fluid received by the first flow path does not flow into a second flow path on the other side with the separation portion as a reference. In the separation portion, the first flow path and the second flow path are separated to block the flow of the fluid. After a certain period of time has passed, in the separation portion, the first flow path and the second flow path are brought into contact, and the fluid is allowed to flow between the first flow path and the second flow path.

[0205] In the method of using the sheet structure, it is preferable that the first flow path is the fluid receiving portion, and a part of the second flow path is provided with the detection portion.

[0206] Hereinafter, Figure 1B , Figures 2A - 2B , a specific description will be given.

[0207] In other words, the above-mentioned "first flow path" is preferably the flow path on the side where the fluid receiving portion for receiving the fluid exists when the flow path is separated with the separation portion as a reference. That is, Figure 1B in Figure 1B , the flow path including flow path A, which preferably exists on the left side in the figure compared with the separation portion X, is preferred.

[0208] In other words, the above-mentioned "second flow path" is preferably the flow path on the side where the detection portion exists, that is, the flow path on the side other than the first flow path side when the flow path is separated with the separation portion as a reference. That is, Figure 1B in Figure 1B , the flow path including flow path B and flow path C, which preferably exists on the right side in the figure compared with the separation portion X, is preferred.

[0209] Here, "in the above-mentioned separation portion, separating the above-mentioned first flow path from the above-mentioned second flow path to block the flow of the above-mentioned fluid", for example, Figure 2A and Figure 2B as shown, it means separating each flow path (porous structure) with the separation portion as a reference.

[0210] The above-mentioned "certain time" can be appropriately set according to the viscosity of the flowing fluid, reaction time such as components contained in the fluid (for example, antigen and antibody), etc.

[0211] Here, "in the above-mentioned separation portion, bringing the above-mentioned first flow path into contact with the above-mentioned second flow path to allow the above-mentioned fluid to flow between the above-mentioned first flow path and the above-mentioned second flow path", for example, it means returning the sheet-like structure in the state of Figure 2A 、 Figure 2B to the state of Figure 1B .

[0212] From the viewpoint of preventing the fluid receiving portion and the detection portion from being adsorbed due to non-flow of the fluid (specimen such as antigen, and reagent such as antibody), etc., a blocking agent can be pre-applied. As such a blocking agent, for example, an aqueous albumin solution, etc. can be cited, and it is preferably appropriately selected according to physical properties such as the type and viscosity of the fluid.

[0213] The sheet-like structure of the present invention can be suitable as an inspection device. As such an inspection device, for example, a pregnancy test drug, an inspection device for a measurement method using the principle of sandwich ELISA method and the principle of chromatography, which is called immunochromatography, etc. can be cited.

[0214] Examples

[0215] Next, examples and comparative examples are listed to more specifically illustrate the present invention, and the present invention is not limited to the following examples.

[0216] <Production of Ink Ribbon>

[0217] The following materials were blended and melt-mixed at 100 °C to prepare a WAX ink.

[0218] · 72.0 parts by mass of paraffin wax (trade name: ParaffinWax-135, manufactured by Nippon Seiro Co., Ltd.) as a hydrophobic material

[0219] · 18.0 parts by mass of synthetic wax (trade name: Diamond Carna (registered trademark) 30, manufactured by Mitsubishi Chemical Corporation) as a hydrophobic material

[0220] · 1.8 parts by mass of carbon black (trade name: MA-100, manufactured by Mitsubishi Chemical Corporation) as a colorant

[0221] · 11.25 parts by mass of resin (trade name: Ultrathene (registered trademark) 722, manufactured by Tosoh Corporation)

[0222] The viscosity of the obtained WAX ink is 23 mPa·s at 140 °C and a shear rate of 3000 s -1 . This viscosity is measured using a rheometer AR-G2 (manufactured by TA instrument).

[0223] The obtained WAX ink is coated on a polyethylene terephthalate film (trade name: lumirror (registered trademark) #6C F531, manufactured by Toray Industries, Inc.) with an average thickness of 6 μm so as to have an average thickness of 5 μm to 12 μm to produce an ink ribbon.

[0224] Using a thermal transfer printer (trade name: Respuri R412v-ex, manufactured by Sato Holdings Corporation), printing is performed on offset paper Figure 13A and Figure 13B the flow path shape shown, thereby forming a missing flow path pattern in the printed portion of the ink ribbon (see Figure 13C and Figure 13D ).

[0225] <Confirmation of flow path formation>

[0226] First, confirm whether the flow path is properly formed.

[0227] Using a printer (trade name: Xerox ColorQube8570, manufactured by XEROX), printing the WAX ink on one side of a filter paper (trade name: Whatman#41) so as to form the flow path pattern shown. Then, in an oven, heating is performed at 120 °C for 2 minutes to allow the WAX ink to penetrate the filter paper and form a single-sided printed flow path. After impregnating the single-sided printed flow path with an aqueous solution of fluorescent ink (fluorescent sign pen, manufactured by ASKUL Corporation), in the same manner as Figure 14A Figure 14A ​Cut at the position corresponding to the f-f' line in [the relevant part], and use a microscope (manufactured by Keyence) to take a cross-sectional photo of the flow path. The result is shown in Figure 14B .

[0228] Similarly, print the flow path pattern shown in Figure 14A on both sides of a filter paper (product name: Whatman #41). Except for this, perform penetration under the same conditions to form a flow path with double-sided printing inside the filter paper. After impregnating the double-sided printed flow path with an aqueous solution of fluorescent ink (fluorescent signature pen, manufactured by ASKUL Corporation), cut at the position corresponding to the f-f' line in Figure 14A [the relevant part], and use a microscope (manufactured by Keyence) to take a cross-sectional photo of the flow path. The result is shown in Figure 14C .

[0229] Figures 14B - 14C In [the relevant part], only the dotted line surrounded part was confirmed to have fluorescent ink, and no leakage of this fluorescent ink was observed in other areas. Therefore, it can be confirmed that the flow path was properly formed. Figure 14B For single-sided printing, that is, WAX ink penetrates from one side of the filter paper, so the formed flow path becomes a shape that expands from the side where the WAX ink penetrates to the opposite side, that is, a conical shape. Figure 14C For double-sided printing, that is, WAX ink penetrates from both sides of the filter paper, and the formed flow path becomes symmetrical and diamond-shaped.

[0230] <Quantitative analysis of paraoxon>

[0231] Paraoxon (or paraoxon) is a kind of phosphorus-based pesticide. When this paraoxon attached to crops and the like enters the body, it hinders the function of acetylcholinesterase (AChE: acetylcholinesterase) related to nerve transmission, and is a nerve poison that causes convulsions, miosis, etc. In this example, a sheet-like structure is used to quantitatively analyze the paraoxon concentration in the sample.

[0232] -Fabrication of the porous structure layer-

[0233] Fix each of the ink tapes with the missing flow path patterns shown in Figure 13C and Figure 13D on the front and back of a filter paper (average thickness 310 μm, basis weight 94 g / m 3 , CFR (Capillary flow rate) 13.9 sec / 4 cm). Then, through a thermal laminating material (product name: GL535ML, manufactured by GBG Corporation) set at a predetermined temperature, transfer and penetrate the WAX ink into the filter paper to form a three-dimensional flow path inside the filter paper, and fabricate a porous structure layer. Here, the "predetermined temperature" is 85 °C with a linear speed of 10 mm / sec during transfer and 85 °C with a linear speed of 5 mm / sec during penetration.

[0234] The obtained porous structure layer has Figure 15 the structure shown. Additionally, Figure 15 is a schematic cross-sectional view when cutting at a position corresponding to the e-e' line shown in Figures 13A - 13D . Here, in the obtained porous structure layer, for convenience, Figure 15 as shown, it is provided with flow path names up to flow paths A to H. Further, the layer having flow path A as the fluid receiving part and flow path B as the detection part is denoted as the first porous structure layer 111, and the layer that is not the first porous structure layer 111 is denoted as the second porous structure layer 211.

[0235] - Sealing and Fixing -

[0236] To prevent the adsorption of AChE (manufactured by Sigma - Aldrich) to the filter paper, 2 μL of a 0.8% aqueous solution of albumin (manufactured by Fujifilm Wako Pure Chemical Corporation) as a blocking agent was dropped into the fluid receiving part (flow path A) and dried at 30 °C for 15 minutes.

[0237] Next, in the detection part (flow path B), 2 μL of an aqueous solution of PDDA (poly(diallyldimethylammonium chloride)) 0.25 (w / v%) (manufactured by Sigma - Aldrich) as an immobilizing agent for indoxyl acetate (IDA) was dropped and dried at 30 °C for 15 minutes. Additionally, IDA is a substrate that turns blue when reacting with AChE.

[0238] Next, after dissolving IDA (manufactured by Sigma - Aldrich) in methanol (manufactured by Fujifilm Wako Pure Chemical Corporation), it was diluted pure, the weight ratio of methanol to water was 70 / 30, and a solution with IDA at 40 mM was prepared. 3 μL of this solution was dropped into the detection part (flow path B) and dried at 30 °C for 15 minutes.

[0239] - Fabrication of the Support Layer -

[0240] In such a way as not to cover flow path A which is the fluid receiving part in the obtained porous structure, adhesive tapes as the support layer (product name: 660PF, manufactured by Nichiban Co., Ltd.) were laminated on both sides of this support layer, and lamination was carried out using a manual roller under room temperature conditions. Here, for convenience, Figure 16 as shown, the support layer on the side of the first porous structure layer 111 is designated as 302, and the support layer on the side of the second porous structure layer 211 is designated as 301.

[0241] Next, a separation part X was formed by cutting between the fluid receiving part (flow path A) and the adjacent flow path C with a cutter, and a sheet-like structure 15 was obtained. At this time, the support layer 301 had no cuts. The obtained sheet-like structure 15 had Figure 16 the structure shown.

[0242] The obtained sheet-like structure 15 was fixed with a bending jig at 180° with the separation part X as a reference as shown in Figure 17 .

[0243] -Reaction-

[0244] AChE was adjusted to be 100 U / mL using Tris-HCl (pH 8.0) (manufactured by Nippon Gene Co., Ltd.), 3 μL was dropped into the fluid receiving part (flow path A), and dried at 30 °C for 15 minutes.

[0245] Tris-HCl (pH 8.0) prepared by adding EtOH (manufactured by Fujifilm Wako Pure Chemical Corporation) to be 6 vol% was used to prepare solutions with paraoxon (manufactured by Sigma-Aldrich) concentrations of 0 μg / L, 200 μg / L, 400 μg / L, and 800 μg / L. Each solution was put into the fluid receiving part (flow path A) and reacted at room temperature.

[0246] After standing for 5 minutes, the jig was removed, and the flow path of the sheet-like structure 15 was contacted again (returning to the Figure 16 state), and the flow of the fluid was continued. After standing for 10 minutes, the color development in the detection part B was measured using a Konica Minolta densitometer FD-05 in the L ＊ a ＊ b ＊ color system. The results are shown in Table 1, Figure 18A , and Figure 18B .

[0247] [Table 1]

[0248]

[0249] Compared with the sample in which the sheet-like structure was not bent at the separation part, for the sample in which the sheet-like structure was bent at the separation part, it was confirmed that the L ＊ value increased and the b ＊ value increased. That is, by bending the sheet-like structure at the separation part to completely block the adjacent flow paths, sufficient reaction time between paraoxon and AChE can be ensured, and the accuracy of quantitative analysis can be improved.

[0250] This international application claims the priority based on Japanese Patent Application No. 2022-188860 filed on November 28, 2022, and incorporates the entire content of Japanese Patent Application No. 2022-188860 into this international application.

[0251] Explanation of symbols

[0252] 11 First sheet-like structure of the first mode

[0253] 12 First sheet-like structure of the second mode

[0254] 13 First sheet-like structure of the third mode

[0255] 14 First sheet-like structure of the fourth mode

[0256] 15 First sheet-like structure of the fifth mode

[0257] 16 First sheet-like structure of the sixth mode

[0258] 17 First sheet-like structure of the seventh mode

[0259] 18 First sheet-like structure of the eighth mode

[0260] 19 First sheet-like structure of the ninth mode

[0261] 101 Porous structure layer

[0262] 102 First porous structure layer

[0263] 103 First porous structure layer

[0264] 104 First porous structure layer

[0265] 105 First porous structure layer

[0266] 106 First porous structure layer

[0267] 107 First porous structure layer

[0268] 108 First porous structure layer

[0269] 109 First porous structure layer

[0270] 110 First porous structure layer

[0271] 111 First porous structure layer

[0272] 202 Second porous structure layer

[0273] 203 Second porous structure layer

[0274] 204 Second porous structure layer

[0275] 205 The second porous structure layer

[0276] 206 The second porous structure layer

[0277] 207 The second porous structure layer

[0278] 208 The second porous structure layer

[0279] 209 The second porous structure layer

[0280] 210 The second porous structure layer

[0281] 211 The second porous structure layer

[0282] 301 Support layer

[0283] 302 Support layer

[0284] 303 Support layer

[0285] 304 Support layer

[0286] A flow path

[0287] B flow path

[0288] C flow path

[0289] D flow path

[0290] E flow path

[0291] E1 flow path

[0292] E2 flow path

[0293] E3 flow path

[0294] E31 flow path

[0295] E32 flow path

[0296] E4 flow path

[0297] F flow path

[0298] G flow path

[0299] H flow path

[0300] X1 flow path

[0301] X2 flow path

[0302] Y non-flow path

[0303] Y1 non-flow path

[0304] M material

[0305] M' material

[0306] X separation part

Claims

1. A sheet-like structure, characterized in that, Having: A porous structure layer with at least a part of a flow path of a porous structure capable of flowing a fluid exposed on the surface, and a support layer, The flow path has a separation part that separates adjacent flow paths and can block the flow of the fluid in the flow path.

2. The sheet-like structure according to claim 1, The separation part is a cut part that cuts the flow path in a direction crossing the flow direction of the fluid in the flow path.

3. The sheet-like structure according to claim 1 or 2, At least a part of the flow path exposed on the surface is a fluid receiving part.

4. The sheet-like structure according to claim 3, The separation part separates the fluid receiving part and the flow path adjacent to the fluid receiving part, and can block the flow of the fluid received by the fluid receiving part to the flow path adjacent to the fluid receiving part.

5. The sheet-like structure according to claim 3 or 4, The support layer is disposed in a region of the porous structure layer that is not located in the fluid receiving part.

6. The sheet-like structure according to any one of claims 1 to 5, The support layer is water-impermeable.

7. The sheet-like structure according to any one of claims 1 to 6, The exposed shape of the flow path exposed on one surface in the porous structure layer is different from the exposed shape of the flow path exposed on the other surface.

8. The sheet-like structure according to any one of claims 3 to 5, At least a part of the flow path exposed on the surface is a detection part, and the detection part is disposed at a position different from the fluid receiving part in the flow path.

9. A method of using a sheet-like structure, characterized in that, It is a method of using the sheet-like structure according to any one of claims 1 to 8, With the separation part as a reference, the first flow path on one side receives the fluid, and in the separation part, the first flow path and the second flow path are separated so that the fluid received by the first flow path does not flow into the second flow path on the other side with the separation part as a reference, blocking the flow of the fluid, After a certain period of time has passed, in the separation part, the first flow path and the second flow path are brought into contact, and the fluid is allowed to flow between the first flow path and the second flow path.

10. The method of using a sheet-like structure according to claim 9, The first flow path is a fluid receiving part, and a detection part is disposed in a part of the second flow path.

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