A method for cultivating termite fungus garden

Abstract

The present invention relates to the technical field of biomimetic cultivation of termite-derived Albizia var. albuminii, and more particularly to a method for cultivating a termite-derived Albizia var. albuminii nursery. The cultivation method comprises the steps of establishing a culture chamber, preparing wood-decaying fungus strains, preparing ant-eating inoculated wood, preparing culture soil for the nursery, preparing wooden culture cups, collecting Albizia var. albuminii strains, cultivating termite nests, and cultivating the nursery. The present invention utilizes wooden culture cups, an intelligently controlled culture chamber, and liquid strains prepared by collecting Albizia var. albuminii spore powder for inoculation to complete the cultivation of the termite-derived Albizia var. albuminii nursery. This method can effectively improve the success rate of cultivating the termite-derived Albizia var. albuminii nursery, shorten the cultivation cycle, reduce labor costs, and is simple and easy to operate. The method represents a key technology for an important step in the biomimetic cultivation of termite-derived Albizia var. albuminii.

Description

Technical Field

[0001] The invention relates to the technical field of bionic cultivation of termite albuminus, and in particular to a cultivation method of a termite albuminus fungus garden. Background Art

[0002] Termitomyces spp. is a rare wild edible mushroom that lives in symbiosis with termites (such as Macrotermes). Its growth depends on the specific ecological environment of the mushroom bed within the termite nest. Termites build the mushroom bed by decomposing organic matter such as lignocellulose, while Termitomyces spp. relies on termite metabolic products and the microenvironment within the bed to grow.

[0003] Currently, the biomimetic cultivation technology of the termite-Altissima symbiotic system is still in the exploratory stage. In existing technologies, researchers have attempted to cultivate Altissima by artificially simulating the termite nest environment, but there are the following key drawbacks:

[0004] The culture container materials are not environmentally friendly: Existing solutions mostly use plastic containers as termite culture nests, which are difficult to degrade naturally. After planting in the field, the reproductive termites (king ants and queen ants) need to be removed and re-nested, which prolongs the fruiting cycle of the Alstonia albuminosa, and the plastic residues pollute the soil environment.

[0005] Inadequate container functionality: While paper cups are somewhat biodegradable as an alternative, they offer poor thermal insulation and moisture retention, making it difficult to maintain the stable microenvironment required for a fungus nursery. This results in inefficient termite nesting and a prolonged incubation period. Furthermore, paper cups are easily damaged by soil moisture, hindering the preservation, migration, and large-scale transportation of fungus nurseries.

[0006] Low inoculation rate of ant nests: The existing technology uses liquid fungus from the fruiting bodies of Alstonia albuminosa to inoculate ant nests. The fungus cultivation operation is complicated and the inoculation rate of ant nests is low. It fails to effectively simulate the efficient symbiotic mode of termites and Alstonia albuminosa in the wild, resulting in low survival rate of the mushroom garden and poor mushroom stability.

[0007] In response to the above problems, it is urgent to develop an artificial cultivation method that takes into account environmental protection, functionality and large-scale production potential. Summary of the Invention

[0008] In view of the shortcomings of the existing technology, the present invention utilizes wooden culture cups and intelligent control culture chambers to complete the cultivation of termite Albizia albuminosa mushroom gardens.

[0009] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0010] The present invention provides a method for cultivating a termite capsicum fungus garden, comprising the following steps:

[0011] Cultivation room establishment: Build an intelligent control cultivation room equipped with control equipment, monitoring equipment and intelligent control system box;

[0012] Preparation of wood-decaying fungus strains: extracting wood-decaying fungus fruiting bodies from dead wood, sterilizing, purifying and culturing to obtain wood-decaying fungus strains, and then diluting to obtain wood-decaying fungus liquid strains;

[0013] Preparation of ant-eating inoculated wood: Process dead wood into 2-3 cm long and 1-2 cm thick wood strips, inoculate the wood strips with liquid spawn of wood-decaying fungi, and then seal and preserve them to obtain ant-eating inoculated wood;

[0014] Preparation of culture soil for mushroom nursery: Collect clay with a pH value of 5.5-6.5, sterilize and adjust the humidity of the clay to 30%-40% to obtain nursery culture soil;

[0015] Preparation of wooden culture cup: Use dry wood with a diameter of 6-8 cm to process it into a wooden culture cup. After sterilization, inoculate the inside of the wooden culture cup with liquid spawn of wood-rotting fungi;

[0016] Collection of Albizia albuminosa strains: Collect Albizia albuminosa fruiting bodies, disinfect and dry them, and then collect Albizia albuminosa spore powder;

[0017] Termite nest cultivation: Place the paired termite breeding ants in a wooden culture cup filled with nursery culture soil and ant food inoculated wood. After sealing, control the environmental parameters of the culture room to complete the cultivation of the termite nest and the differentiation of larvae.

[0018] Mushroom nursery cultivation: Prepare the spore powder of Alstonia albuminosa into liquid fungus and inoculate it into the termite nest. Adjust the temperature and humidity to cultivate for 25-35 days. After forming a termite-alstonia albuminosa symbiotic fungus nursery, transplant it into the wild for planting.

[0019] Furthermore, the cultivation room is set up including: the control equipment includes an air energy temperature control device, a humidifier, a fumigator and a bio-light strip;

[0020] The monitoring equipment includes a temperature and humidity sensor, a light sensor and a carbon dioxide sensor;

[0021] The information monitored by the temperature and humidity sensors, light sensors, and carbon dioxide sensors is transmitted to the intelligent control system box, and the intelligent control system box regulates the air temperature control device, humidifier, fumigator, and biological light strip according to the received monitoring information.

[0022] Furthermore, the wood-rotting fungus strain is prepared as follows:

[0023] The mother species of wood-decaying fungi are isolated from dead wood of Yunnan pine, fir or oak, and purified after surface disinfection to obtain purified strains;

[0024] After culturing the purified strain in a culture medium for 14 days, the culture medium was transferred to a shake flask for shaking culture for 5-8 days at room temperature of 22°C-26°C. The culture medium formula comprises: 20 grams of potato starch, 10 grams of corn flour, 10 grams of wheat flour, 5 grams of mung bean flour, 5 grams of yeast extract powder, 5 grams of peptone, 5 grams of potassium dihydrogen phosphate, 5 grams of magnesium sulfate, 20 grams of glucose, 3 grams of trehalose, 11 grams of vitamin B1, 1 gram of kinetin, and 10 grams of agar.

[0025] After the mycelium forms primordium, it is diluted with sterile water to form liquid culture of wood-decaying fungus.

[0026] Furthermore, the preparation of the ant-eating inoculated wood is specifically as follows:

[0027] Cut dry wood of Yunnan pine, fir or oak into strips 2-3 cm long and 1-2 cm thick;

[0028] Soak the wood strips in clean water for 36-52 hours and then inoculate them with liquid spawn of wood-decaying fungi. After packaging, store them at room temperature for 20 days to make ant-eating inoculated wood.

[0029] Furthermore, the mushroom garden culture soil is prepared specifically by sterilizing red clay or yellow clay at a high temperature of 121° C. and adjusting the soil moisture to 30%-40% to obtain the mushroom garden culture soil.

[0030] Furthermore, the production of the wooden culture cup includes:

[0031] Process dry wood of Yunnan pine, fir or oak with a diameter of 6-8 cm into 10 cm long wood segments, and then turn them into wooden cups with an outer diameter of 6-8 cm, an inner diameter of 5-7 cm and a depth of 8 cm;

[0032] The wooden cup was sterilized by high pressure sterilization at 121° C. and 0.15 MPa to obtain a wooden culture cup.

[0033] Furthermore, the collection of the Albizia albuminosa strains is specifically as follows: the sterilized Albizia albuminosa fruiting bodies are placed in a sealed glass container for drying, and the Albizia albuminosa spore powder is collected and stored.

[0034] Furthermore, the termite nest cultivation includes:

[0035] Fill the wooden culture cups with nursery culture soil and ant food inoculated wood in layers from bottom to top, and place a pair of wingless breeding ants in each cup;

[0036] Seal and fix the wooden culture cup in a light-proof manner, and poke 3-5 ventilation holes in the seal;

[0037] The temperature, humidity, light intensity and carbon dioxide concentration in the culture room are regulated in stages to cultivate termite nests.

[0038] Furthermore, the temperature, humidity, light intensity and carbon dioxide concentration of the culture room are regulated in stages to cultivate the termite nests.

[0039] The termite nest was placed in an incubation room with a temperature of 25°C, a humidity of 65%, a light intensity of 1000-1500 Lux, and a carbon dioxide concentration of 800-1000 ppm for 8 days until the queen ant laid eggs;

[0040] After spawning, adjust the culture room temperature to 25-26°C, humidity to 50%-60%, light intensity to 1500-2000 Lux, and carbon dioxide concentration to 1000-1200 ppm, and continue incubation for 18-24 days;

[0041] After the eggs hatch into young ants, adjust the temperature of the culture room to 26℃-27℃, humidity 50-60%, light intensity 2000-2500Lux, and carbon dioxide concentration 1200-1500ppm. Continue to cultivate for 35 days. The young ants molt and grow into nymphs, and the nymphs differentiate into worker ants and soldier ants. The termite nest cultivation is completed.

[0042] Furthermore, the mushroom garden cultivation is specifically as follows:

[0043] Mix the spore powder of Alternaria albuminosa with sterile water at a mass ratio of 1:1000 and inject it onto the surface of the termite nest.

[0044] The culture room temperature was regulated at 27-28°C, humidity at 50%-60%, light intensity at 2000-2500 Lux, and carbon dioxide concentration at 1200-1500 ppm. Culture was continued for 28-33 days until the mycelium formed symbiotic white balls.

[0045] Compared with the prior art, the present invention has the following advantages:

[0046] The present invention cultivates a termite chicken mushroom garden by using an intelligent control system, which can accurately and intelligently control the spatial temperature, humidity, light and CO2 concentration of termites in different growth and breeding stages, accelerate the termite egg-laying, hatching, ant colony growth and nesting processes, and reduce manual management costs; the invention utilizes forest residues to make culture cups for termite cultivation, which has good heat preservation and moisture retention effects and is closer to the wild living environment of termite cultivation, thereby improving the termite cultivation survival rate and shortening the mushroom garden cultivation time, while effectively utilizing forest residues and reducing the risks of forest fires; the invention provides termites with easily digestible and absorbable ant food for inoculation, which is beneficial to the survival and growth of the ant colony; the invention uses a technology for collecting chicken mushroom spore powder for inoculation, which is simple to operate, not easily infected by miscellaneous bacteria, and has a high success rate for inoculating ant nests; after the wooden chicken mushroom culture cups for termites are planted in the wild, the wooden culture cups can directly become termite food, thereby reducing the re-nesting process and shortening the fruiting time of the chicken mushroom. This method is low-cost, easy to operate, has a short cycle and a high success rate. It shortens the cultivation cycle of the chicken mushroom garden from 10 months with existing cultivation technology to 4 months, increases the survival rate from about 40% to about 90%, and shortens the time for mushroom fruiting after wild cultivation in the garden from 4 years to 3 years. BRIEF DESCRIPTION OF THE DRAWINGS

[0047] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following is a brief introduction to the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0048] Figure 1 A diagram of a culture chamber in an embodiment of the present invention is shown;

[0049] Figure 2 Shows a diagram of a temperature and humidity sensor in an embodiment of the present invention;

[0050] Figure 3 A diagram of a carbon dioxide sensor according to an embodiment of the present invention is shown;

[0051] Figure 4 A diagram of a light sensor according to an embodiment of the present invention is shown;

[0052] Figure 5 Shows a box diagram of an intelligent control system in an embodiment of the present invention;

[0053] Figure 6 The wooden culture cup in Example 1 of the present invention is shown;

[0054] Figure 7 The plastic culture cup in Example 2 of the present invention is shown;

[0055] Figure 8The paper culture cup in Example 3 of the present invention is shown. DETAILED DESCRIPTION

[0056] To make the objectives, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention are clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention. Example 1:

[0057] S1: Cultivation room establishment: the establishment area is 60m 2 Unit culture room (such as Figure 1 As shown in the figure, the culture room is a clean room with a length of 10m, a width of 6m and a height of 3m. It is equipped with multi-layer carbon steel culture racks with a spacing of 60cm between layers and a spacing of 60cm between each row of culture racks. Control equipment is installed, including one set of air energy temperature control device, one set of humidifier, one set of fumigator and one set of biological light strip. Monitoring equipment is also installed, including temperature and humidity sensors (such as Figure 2 As shown), carbon dioxide sensor (as Figure 3 As shown), light sensor (as Figure 4 As shown); and intelligent control system box (as Figure 5 The intelligent control system box is used to control the temperature, humidity, carbon dioxide concentration and light intensity in the culture room.

[0058] The carbon dioxide sensor is used to monitor the carbon dioxide concentration information in the culture room and transmit it to the intelligent control system box. Depending on whether the carbon dioxide concentration information reaches the preset concentration range, if it is not within the preset concentration range, a control command will be sent to the fumigator to regulate the carbon dioxide concentration in the culture room. Specifically, the preset concentration range of carbon dioxide is 800-1500ppm.

[0059] The temperature and humidity control sensor is used to monitor the temperature and humidity information in the culture room and transmit it to the intelligent control system box. If the temperature and / or humidity in the culture room are not within the preset range, the control command will be sent to the air energy temperature control device and / or humidifier to regulate the temperature and humidity in the culture room. Specifically, the temperature of the culture room is controlled at 23-28°C and the humidity is 60%.

[0060] The light sensor is used to monitor the light information in the culture chamber and transmit it to the intelligent control system box. If the light intensity in the culture chamber is not within the preset concentration range, the control command will be sent to the bio-light strip to regulate the light intensity in the culture chamber. Specifically, the light intensity is 1000-2500Lux.

[0061] S2: Preparation of Wood-Destroying Fungus Cultures: Wood-destroying fungus mother cultures are collected from wild rotting woods such as Yunnan pine, fir, and oak. After disinfection, the culture is extracted and purified on a sterile operating table. Culture is performed in test tubes using culture medium for 14 days, then transferred to shake flasks and shaken for 7 days at a controlled temperature of 25°C. After primordia are formed, the culture is diluted with sterile water to form a liquid culture medium. Culture medium formula: 20g potato starch, 10g corn flour, 10g wheat flour, 5g mung bean flour, 5g yeast extract powder, 5g peptone, 5g potassium dihydrogen phosphate, 5g magnesium sulfate, 20g glucose, 3g trehalose, 11g vitamin B1, 1g kinetin, and 10g agar.

[0062] S3: Preparation of Ant-Eating Inoculated Wood: Dry wood of Yunnan pine, fir, or oak is collected and cut into logs approximately 2-3 cm thick. The logs are then split into strips 2-3 cm long and 1-2 cm thick. The wood is then soaked in clean water for 48 hours, inoculated with liquid spawn of wood-decaying fungi, and placed in a plastic bag and stored at room temperature for 20 days to prepare the Ant-Eating Inoculated Wood.

[0063] S4: Preparation of culture soil for mushroom nursery: Collect clean red clay or yellow clay with a pH value of 5.5-6.5, sterilize it at high temperature and add water to adjust the soil moisture to 35% to obtain nursery culture soil.

[0064] S5: Preparation of wooden culture cups: Collect dry wood (such as Yunnan pine, fir, oak, etc.) with a diameter of about 6-8 cm, cut it into 10 cm long sections with a cutter or electric saw, and use a wood turning machine to process it into a wooden cup with an outer diameter of about 6-8 cm, an inner diameter of 5-7 cm, and a depth of about 8 cm. After sterilizing the wooden cups at high temperature and high pressure, the wooden culture cups (such as Figure 6 As shown), the interior of the wooden culture cup is inoculated with the corresponding liquid strain of wood-rotting fungi.

[0065] S6: Collection of Albizia albuminosa strains: Select excellent varieties of Albizia albuminosa fruiting bodies with strong growth, sterilize them, place them in a glass container with a length of 1m, a width of 0.4m and a height of 0.5m, absorb moisture with a desiccant, collect the spore powder emitted by the fruiting body with a scalpel, store it in a 25mL storage cup, and place the cup in a 3℃ refrigerator for later use.

[0066] S7: Cultivation of termite nests: Pour the prepared culture soil into the wooden culture cup to 1 / 3 of the cup, then add 3-4 pieces of ant food inoculation wood, and continue to add culture soil to 2 / 3 of the cup; capture the breeding termites cultured with Alstonia albuminosa, remove their wings and pair them up, put one pair of mated ants (one female and one male) into each cup and let them bury themselves in the soil to build a nest, seal the cup mouth with a 10cm×10cm black plastic film and a rubber band, and use a needle to poke 3-5 small holes in the middle of the film for ventilation. Place the termite nest in a culture room with a temperature of 25℃, a humidity of 65%, a light intensity of 1000-1500Lux, and a carbon dioxide concentration of 800-1000ppm for incubation for 8 days until the queen ant lays eggs; after laying eggs, adjust the temperature of the culture room to 25℃-26℃, the humidity of 50%-60%, the light intensity of 1500-2000Lux, and the carbon dioxide concentration of 1000-1200ppm, and continue incubation for 18-24 days; after the eggs hatch into young ants, adjust the temperature of the culture room to 26℃-27℃, the humidity of 50-60%, the light intensity of 2000-2500Lux, and the carbon dioxide concentration of 1200-1500ppm, and continue incubation for 35 days. The young ants molt and grow into nymphs, and the nymphs differentiate into worker ants and soldier ants, and the termite nest cultivation is completed.

[0067] S8: Cultivation of mushroom garden: When the worker ants in the termite nest begin to eat the inoculated wood, mix the spore powder of Alstonia albuminosa with sterile water at a mass ratio of 1:1000 to prepare a liquid fungus, and inject it into the termite nest with a syringe. Adjust the temperature of the culture room to 27℃-28℃, the humidity to 50%-60%, the light intensity to 2000-2500Lux, and the carbon dioxide concentration to 1200-1500ppm. The humidity of the culture soil in the cup is controlled between 30-40%. Continue to cultivate for about 30 days. When small white balls of Alstonia albuminosa grow on the termite nest, the symbiotic relationship between termites and Alstonia albuminosa is established, and the termite Alstonia albuminosa garden is successfully cultivated and can be transplanted into the wild for planting. Example 2:

[0068] The termite capsicum fungus garden was cultivated according to the method of Example 1, except that the operation process of S5, S6 and S8 was operated in the following manner:

[0069] S5: Make a plastic culture cup: Use a plastic cup with a diameter of 8 cm and a height of about 10 cm as the culture cup (such as Figure 7 shown).

[0070] S6: Collection of Albizia albuminosa spores: Collect high-quality Albizia albuminosa fruiting bodies from the field. After disinfection, cut the caps into 3 mm × 3 mm pieces. Use an inoculation hook to inoculate these pieces onto a test tube culture medium with a pH of 5.5-6.5 (the culture medium formula is 1000 mL of sterile water, 60 g of starch, 60 g of glucose, 6 g of potassium dihydrogen phosphate, 6 g of magnesium sulfate, 6 g of yeast extract powder, 3 g of glutamic acid, 10 g of agar powder, 5 g of beef extract, and 1 g of vitamin B1). The culture medium is then incubated in a light-shielded incubator at 26°C. After 90 days of incubation, white spherical cells will grow on the culture medium. These cells will be purified to form a solid culture. The solid culture and culture medium are placed in a sterilized Erlenmeyer flask at a mass ratio of 1:30. The mixture is stirred on a magnetic stirrer at 130 rpm for 5-8 days to form a liquid culture for later use.

[0071] S8: Cultivate the mushroom bed: Use a syringe to inject the prepared liquid spawn of Albizia albuminosa onto the ant nest. Continue to cultivate in the incubation room for 60 days to form a termite Albizia albuminosa bed, which can be transplanted into the wild for cultivation. Example 3:

[0072] The termite capsicum fungus garden was cultivated according to the method of Example 1, except that the operation process of S5, S6 and S8 was operated in the following manner:

[0073] S5 Make a paper culture cup: Use a paper cup with a diameter of 8 cm and a height of 10 cm as the culture cup (such as Figure 8 shown).

[0074] S6 Collection of Albizia albuminosa spores: High-quality Albizia albuminosa fruiting bodies were collected from the field. After disinfection, the caps were cut into 3 mm × 3 mm pieces. These were inoculated using an inoculating hook onto a test tube culture medium with a pH of 5.5-6.5 (the culture medium formula is 1000 mL of sterile water, 60 g of starch, 60 g of glucose, 6 g of potassium dihydrogen phosphate, 6 g of magnesium sulfate, 6 g of yeast extract powder, 3 g of glutamic acid, 10 g of agar powder, 5 g of beef extract, and 1 g of vitamin B1). The culture medium was then incubated in a light-shielded incubator maintained at 26°C. After 90 days of incubation, white spherical cells grew on the culture medium. These cells were purified to form a solid culture medium. The solid culture medium was placed in a sterilized Erlenmeyer flask at a mass ratio of 1:30 to the culture medium. The mixture was stirred on a magnetic stirrer at 130 rpm for 5-8 days to form a liquid culture medium for later use.

[0075] S8: Cultivate the mushroom bed: Use a syringe to inject the prepared liquid spawn of Albizia albuminosa onto the ant nest. Continue to cultivate in the incubation room for about 90 days to form a termite Albizia albuminosa bed, which can be transplanted into the wild for cultivation. Example 4:

[0076] The termite collet mushroom garden was cultivated according to the method of Example 1, except that the operation process of S6 and S8 was operated in the following manner:

[0077] S6: Collection of Albizia albuminosa spores: Collect high-quality Albizia albuminosa fruiting bodies from the field. After disinfection, cut the caps into 3 mm × 3 mm pieces. Use an inoculation hook to inoculate these pieces onto a test tube culture medium with a pH of 5.5-6.5 (the culture medium formula is 1000 mL of sterile water, 60 g of starch, 60 g of glucose, 6 g of potassium dihydrogen phosphate, 6 g of magnesium sulfate, 6 g of yeast extract powder, 3 g of glutamic acid, 10 g of agar powder, 5 g of beef extract, and 1 g of vitamin B1). The culture medium is then incubated in a light-shielded incubator at 26°C. After 90 days of incubation, white spherical cells will grow on the culture medium. These cells will be purified to form a solid culture. The solid culture and culture medium are placed in a sterilized Erlenmeyer flask at a mass ratio of 1:30. The mixture is stirred on a magnetic stirrer at 130 rpm for 5-8 days to form a liquid culture for later use.

[0078] S8: Cultivate the mushroom bed: Use a syringe to inject the prepared liquid spawn of Albizia albuminosa onto the ant nest. Continue to cultivate in the incubation room for 60 days to form a termite Albizia albuminosa bed, which can be transplanted into the wild for cultivation.

[0079] From 2021 to the present, the methods in Examples 1 to 4 were used to carry out termite chicken mushroom nursery cultivation experiments at the test site in Yongsheng County, Lijiang. The nursery emergence time, termite survival rate and mushroom emergence time during the cultivation process were statistically analyzed. The test results are shown in Table 1.

[0080] Table 1

[0081]

[0082] As can be seen from the above table, the cultivation method of the termite albuminus mushroom garden proposed in the present invention shortens the cultivation period of the albuminus mushroom garden from 6-10 months of the existing cultivation technology to 4 months, increases the survival rate from 43.6%-60.9% to 90.1%, and shortens the fruiting time after the mushroom garden is planted in the wild from 4 years to 3 years. It has the advantages of low cost, easy operation, short cycle, and high success rate.

[0083] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims

1. A method for cultivating a termite fungus garden, characterized in that: The following steps are involved: Cultivation room establishment: Build an intelligent control cultivation room equipped with control equipment, monitoring equipment and intelligent control system box; Preparation of wood-decaying fungus strains: extracting wood-decaying fungus fruiting bodies from dead wood, sterilizing, purifying and culturing to obtain wood-decaying fungus strains, and then diluting to obtain wood-decaying fungus liquid strains; Preparation of ant-eating inoculated wood: Cut dry wood of Yunnan pine, fir or oak into strips 2-3 cm long and 1-2 cm thick; soak the strips in clean water for 36-52 hours, then inoculate with liquid spawn of wood-decaying fungi, seal them, and store them at room temperature for 20 days to prepare ant-eating inoculated wood; Preparation of culture soil for mushroom nursery: Collect clay with a pH value of 5.5-6.5, sterilize and adjust the humidity of the clay to 30%-40% to obtain nursery culture soil; Preparation of wooden culture cup: Use dry wood with a diameter of 6-8 cm to process it into a wooden culture cup. After sterilization, inoculate the inside of the wooden culture cup with liquid spawn of wood-rotting fungi; Collection of Albizia albuminosa strains: Collect Albizia albuminosa fruiting bodies, disinfect and dry them, and then collect Albizia albuminosa spore powder; Termite nest cultivation: Paired breeding termites are placed in a wooden culture cup filled with nursery soil and ant food inoculated wood. After sealing, the incubation room environmental parameters are regulated to complete the termite nest cultivation and larval differentiation. The wooden culture cup is filled with nursery soil and ant food inoculated wood in layers from bottom to top, and a pair of wingless breeding ants are placed in each cup. The wooden culture cup is sealed and fixed in a light-proof manner, and 3-5 ventilation holes are punched in the seal. The temperature, humidity, light intensity, and carbon dioxide concentration in the incubation room are regulated in stages to cultivate the termite nest. Mushroom nursery cultivation: The spore powder of Alstonia albuminosa is prepared into a liquid fungus, which is then inoculated into a termite nest. The temperature and humidity are controlled and cultured for 25-35 days to form a termite-alstonia symbiotic fungus nursery, which is then transplanted into the wild for planting. This includes: mixing the spore powder of Alstonia albuminosa with sterile water at a mass ratio of 1:1000, and injecting the mixture onto the surface of the termite nest. The culture room temperature was regulated at 27-28°C, humidity at 50%-60%, light intensity at 2000-2500 Lux, and carbon dioxide concentration at 1200-1500 ppm. Culture was continued for 28-33 days until the mycelium formed symbiotic white balls.

2. The method according to claim 1, characterized in that The cultivation room is set up including: the control equipment includes an air energy temperature control device, a humidifier, a fumigator and a biological light strip; The monitoring equipment includes a temperature and humidity sensor, a light sensor and a carbon dioxide sensor; The information monitored by the temperature and humidity sensors, light sensors and carbon dioxide sensors is transmitted to the intelligent control system box, and the intelligent control system box regulates the air temperature control device, humidifier, fumigator and biological light strip according to the received monitoring information.

3. The method according to claim 1, characterized in that The wood-rotting fungus strain is prepared as follows: The mother species of wood-decaying fungi are isolated from dead wood of Yunnan pine, fir or oak, and purified after surface disinfection to obtain purified strains; After culturing the purified strain in a culture medium for 14 days, the strain was transferred to a shake flask for shaking culture for 5-8 days at room temperature of 22°C-26°C. The culture medium comprises: 20 g potato starch, 10 g corn flour, 10 g wheat flour, 5 g mung bean flour, 5 g yeast extract powder, 5 g peptone, 5 g potassium dihydrogen phosphate, 5 g magnesium sulfate, 20 g glucose, 3 g trehalose, 1 g vitamin B1, 1 g kinetin, and 10 g agar. After the mycelium forms primordium, it is diluted with sterile water to form liquid culture of wood-decaying fungus.

4. The method according to claim 1, wherein The mushroom garden culture soil is prepared specifically as follows: the collected red clay or yellow clay is sterilized at a high temperature of 121° C., and then the soil moisture is adjusted to 30%-40% to obtain the mushroom garden culture soil.

5. The method according to claim 1, wherein The wooden culture cup is made specifically as follows: Processing Yunnan pine, fir or oak dry wood with a diameter of 6-8 cm into 10 cm long wood segments, and further turning them into wooden cups with an outer diameter of 6-8 cm, an inner diameter of 5-7 cm and a depth of 8 cm; The wooden cup was sterilized by high pressure sterilization at 121° C. and 0.15 MPa to obtain a wooden culture cup.

6. The method according to claim 1, characterized in that The specific steps of collecting the strains of Albizia albuminosa are as follows: the sterilized fruiting bodies of Albizia albuminosa are placed in a sealed glass container for drying, and the spore powder of Albizia albuminosa is collected and stored.

7. The method according to claim 1, characterized in that The temperature, humidity, light intensity and carbon dioxide concentration of the culture room are regulated in stages to cultivate termite nests. The termite nest was placed in an incubation room with a temperature of 25°C, a humidity of 65%, a light intensity of 1000-1500 Lux, and a carbon dioxide concentration of 800-1000 ppm for 8 days until the queen ant laid eggs; After spawning, adjust the culture room temperature to 25-26°C, humidity to 50%-60%, light intensity to 1500-2000 Lux, and carbon dioxide concentration to 1000-1200 ppm, and continue incubation for 18-24 days; After the eggs hatch into young ants, adjust the temperature of the culture room to 26℃-27℃, humidity 50-60%, light intensity 2000-2500Lux, and carbon dioxide concentration 1200-1500ppm. Continue to cultivate for 35 days. The young ants molt and grow into nymphs, and the nymphs differentiate into worker ants and soldier ants. The termite nest cultivation is completed.

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