Low-protein adsorption surface material and preparation method thereof

Abstract

The invention relates to the technical field of biology, in particular to a low-protein adsorption surface material and a preparation method thereof. The invention provides a preparation method of a low-protein adsorption surface material. The preparation method comprises the following steps: (1) carrying out low-temperature plasma treatment on the surface of a to-be-treated material; (2) coating the surface of a to-be-treated material with the low-protein adsorption dissolving solution; the low-protein adsorption dissolving solution comprises a substance A, a substance B and a photoinitiator, the substance A is at least one of compounds containing at least one hydroxyl group; the substance B is a polyester polymer with a polyether chain segment; (3) carrying out ultraviolet polymerization on the surface of the to-be-treated material coated in the step (2); (4) the surface of the to-be-treated material obtained in the step (3) is subjected to high-energy electron beam irradiation; the material obtained in the scheme can keep low adsorption of protein and polypeptide in a low-temperature environment for a long time.

Description

Technical Field

[0001] The present invention relates to the field of biotechnology, and particularly to a low-protein adsorption surface material and a preparation method thereof. Background Art

[0002] In the fields of life science research and biomedical experiments, the processing and storage of samples are of great importance. Experimental consumables made of traditional polypropylene (PP) and polystyrene (PS) materials are prone to non-specific adsorption of proteins in biological samples due to the presence of polar groups on the material surface. This physical adsorption phenomenon not only significantly reduces the sensitivity of molecular detection, but may also lead to the following chain reactions: 1) irreversible sample loss in micro-sample experiments; 2) non-specific binding of cell surface proteins during cell culture / separation; 3) change in the surface charge distribution of cell membranes, resulting in a decrease in cell recovery rate; 4) interference with cell signal transduction pathways, affecting subsequent functional research.

[0003] Current commercial low-protein adsorption solutions mainly rely on two major technical routes:

[0004] Surface modification technology: A hydrophilic coating is constructed on the surface of PP / PS substrates through silanization treatment, and chemical modification is used to reduce the contact angle of the material surface. However, this technology has defects in coating stability - long-term contact with organic solvents or repeated freezing and thawing will cause the precipitation of siloxane molecules, resulting in secondary contamination of samples and poor durability.

[0005] Fluoropolymer solution: Fluorine-containing materials such as polytetrafluoroethylene (PTFE) are injection-molded, and the passive anti-adsorption is achieved by the unique low surface energy characteristics of fluorine atoms. However, this process requires high-temperature pyrolysis of fluorine-containing monomers, generates hydrogen fluoride gas during the production process, and the raw material cost is higher than that of traditional plastics. Summary of the Invention

[0006] Therefore, the technical problem to be solved by the present invention is to provide a low-protein adsorption surface material and a preparation method thereof, and the prepared low-protein adsorption surface material has strong durability, no risk of gas evolution, is non-toxic, and is easy to produce.

[0007] To this end, the present invention provides the following technical solutions:

[0008] The present invention provides a preparation method of a low-protein adsorption surface material, comprising:

[0009] (1) subjecting the surface of the material to be treated to low-temperature plasma treatment;

[0010] (2), coat the surface of the material to be treated with a low-protein adsorption solution; the low-protein adsorption solution includes substance A, substance B and a photoinitiator; substance A is at least one of compounds containing at least one hydroxyl group; substance B is a polyester polymer including a polyether segment;

[0011] (3), perform ultraviolet polymerization on the surface of the coated material to be treated in step (2);

[0012] (4), irradiate the surface of the material to be treated obtained in step (3) with a high-energy electron beam.

[0013] Optionally, the irradiation dose of the high-energy electron beam irradiation is 15 - 40 KGy, substance A is at least one of polyvinyl alcohol, polyvinylpyrrolidone, polyethylene glycol, and the molecular weight of the polyethylene glycol is 2000 - 35000.

[0014] Optionally, the molecular weight of the polyethylene glycol is 3000 - 30000.

[0015] Optionally, the polyester polymer including a polyether segment is at least one of polyethylene glycol diacrylate and poly(ethylene glycol) methyl ether methacrylate.

[0016] Optionally, the photoinitiator includes at least one of photoinitiator 369, photoinitiator 907, and Irgacure 2959.

[0017] Optionally, in the low-protein adsorption solution, substance A is 0.5 - 5 parts by weight, substance B is 0.25 - 2.5 parts by weight, the photoinitiator is 0.025 - 0.25 parts by weight, and deionized water is 92.25 - 99.25 parts by weight.

[0018] Optionally, the conditions for the ultraviolet polymerization are to perform ultraviolet polymerization at room temperature, the ultraviolet wavelength is 254 nm or 365 nm, and the polymerization time is 10 min - 60 min.

[0019] Optionally, the material to be treated in step (1) is selected from at least one of polystyrene, polypropylene, polycarbonate, and polyethylene terephthalate.

[0020] Optionally, the low-temperature plasma in step (1) is one or more of oxygen, nitrogen, and argon;

[0021] And / or, the treatment power of the plasma treatment in step (1) is 500 W - 2000 W, and the treatment time is 200 s - 2000 s.

[0022] The present invention provides a low-protein adsorption surface material prepared by the preparation method of the low-protein adsorption surface material described above.

[0023] The technical solution of the present invention has the following advantages:

[0024] 1. A preparation method of a low-protein adsorption surface material provided by the present invention includes: (1) subjecting the surface of the material to be treated to low-temperature plasma treatment; (2) coating the surface of the material to be treated with a low-protein adsorption solution; the low-protein adsorption solution includes substance A, substance B and a photoinitiator; substance A is at least one of compounds containing at least one hydroxyl group; substance B is a polyester polymer including a polyether segment; (3) subjecting the surface of the material to be treated coated in step (2) to ultraviolet polymerization; (4) irradiating the surface of the material to be treated obtained in step (3) with a high-energy electron beam; in the above solution, first, the surface of the material to be treated is subjected to low-temperature plasma treatment to reduce the surface energy of the material, making the solution easier to spread and adhere on the surface, presenting good flatness and reducing the dosage of the solution. Then, a solution containing substance A which is at least one of compounds containing at least one hydroxyl group, a polyester polymer with a polyether segment and a photoinitiator is coated on the surface of the material to be treated after low-temperature plasma treatment. Through ultraviolet polymerization, the photoinitiator is activated to generate free radicals, and the free radicals form active centers with the monomers of substance A and substance B, causing the macromolecules (substance B) of the surface coating to undergo self-crosslinking to form chemical covalent bonds, forming a crosslinked three-dimensional network, and substance A enters this network through this effect and is bonded by hydrogen bonds. Then, the free radicals propagate through vinyl groups (derived from substance B), and further form a crosslinked network. Finally, a 3D network hydrogel is formed through free radical chain polymerization. Finally, the surface of the material to be treated is irradiated with a high-energy electron beam, causing substance A molecules to generate free radicals, and the free radicals undergo self-crosslinking, resulting in a higher crosslinking density and forming a denser network structure, which is manifested as a lower swelling rate and higher strength, making the obtained material surface more suitable for long-term storage of protein and polypeptide samples, avoiding the adsorption of protein and polypeptide samples on the material surface, and can maintain low adsorption of protein and polypeptide under long-term low-temperature environment.

[0025] 2. A preparation method of a low-protein adsorption surface material provided by the present invention, the irradiation dose of the high-energy electron beam irradiation is 15 - 40 KGy. In this dose range, it mainly ensures the sterility level and the excitation of free radicals of substance A. Too high is likely to cause the product to turn yellow, and too low cannot ensure the sterility level.

[0026] 3. A preparation method of a low-protein adsorption surface material provided by the present invention, the molecular weight of the polyethylene glycol is 3000 - 30000. By controlling the molecular weight of the polyethylene glycol within the above range, protein adsorption can be significantly resisted. Detailed implementation manners

[0027] The following embodiments are provided to better understand the present invention further. It is not limited to the described best mode, and does not limit the content and protection scope of the present invention. Any product that is the same or similar to the present invention obtained by anyone under the inspiration of the present invention or by combining the features of the present invention with other prior art features falls within the protection scope of the present invention.

[0028] For those not specifying specific experimental steps or conditions in the embodiments, the operations or conditions of the conventional experimental steps described in the literature in this field can be followed. For the reagents or instruments not indicating the manufacturer, they are all conventional reagent products that can be obtained through commercial purchase.

[0029] The present invention provides a method for preparing a low-protein adsorption surface material, comprising:

[0030] (1) subjecting the surface of the material to be treated to low-temperature plasma treatment;

[0031] (2) coating the surface of the material to be treated with a low-protein adsorption solution; the low-protein adsorption solution comprises substance A, substance B, and a photoinitiator; substance A is at least one of compounds containing at least one hydroxyl group; substance B is a polyester polymer comprising a polyether segment;

[0032] (3) subjecting the surface of the coated material to be treated in step (2) to ultraviolet polymerization;

[0033] (4) irradiating the surface of the material to be treated obtained in step (3) with high-energy electron beams.

[0034] In the above method, first, the surface of the material to be treated is subjected to low-temperature plasma treatment to reduce the surface energy of the material, making the solution easier to spread and adhere on the surface, presenting good flatness and reducing the amount of the solution used. Then, a solution containing substance A (at least one of compounds containing at least one hydroxyl group), a polyester polymer with a polyether segment, and a photoinitiator is coated on the surface of the material to be treated after low-temperature plasma treatment. Through ultraviolet polymerization, the photoinitiator is activated to generate free radicals, and the free radicals form active centers with the monomers of substance A and substance B, causing the macromolecules (substance B) of the surface coating to undergo self-crosslinking to form chemical covalent bonds, forming a crosslinked three-dimensional network, and substance A enters this network through hydrogen bonding. Then, the free radicals propagate through vinyl groups (derived from substance B), and further form a crosslinked network. Finally, a 3D network hydrogel is formed through free radical chain polymerization. Finally, high-energy electron beam irradiation is used to cause substance A molecules to generate free radicals, and the free radicals undergo self-crosslinking, resulting in a higher crosslinking density and forming a denser network structure, which is manifested as a lower swelling rate and higher strength, making the obtained material surface more suitable for the long-term storage of protein and polypeptide samples.

[0035] In some embodiments, the irradiation dose of the high-energy electron beam irradiation is 15 - 40 KGy. For example, the irradiation dose of the high-energy electron beam irradiation can be any value among 15, 18, 20, 23, 25, 30, 32, 35, 37, 40 KGy or the range value between any two values.

[0036] In some embodiments, the substance A is at least one of polyvinyl alcohol, polyvinylpyrrolidone, and polyethylene glycol;

[0037] In a preferred embodiment, the molecular weight of the polyethylene glycol is 2000 - 35000; in a more preferred embodiment, the molecular weight of the polyethylene glycol is 3000 - 30000.

[0038] In some embodiments, the polyester polymer of the polyether segment is at least one of polyethylene glycol diacrylate and poly(ethylene glycol) methyl ether methacrylate.

[0039] In some embodiments, the photoinitiator includes at least one of photoinitiator 369, photoinitiator 907, and Irgacure 2959.

[0040] In some embodiments, in the low-protein adsorption solution, the substance A is 0.5 - 5 parts by weight, the substance B is 0.25 - 2.5 parts by weight, the photoinitiator is 0.025 - 0.25 parts by weight, and deionized water is 92.25 - 99.25 parts by weight.

[0041] In some embodiments, the conditions for ultraviolet polymerization are as follows: ultraviolet polymerization is carried out at room temperature, the ultraviolet wavelength is 254 nm or 365 nm, and the polymerization time is 10 min - 60 min. In some embodiments, the polymerization time can be any value among 10, 15, 20, 25, 30, 35, 40, 45, 50, 60 min or the range value between any two values.

[0042] In some embodiments, the material to be treated in step (1) is selected from at least one of polystyrene, polypropylene, polycarbonate, and polyethylene terephthalate.

[0043] In some embodiments, the low-temperature plasma in step (1) is one or more of oxygen, nitrogen, and argon;

[0044] And / or, the processing power of the plasma treatment in step (1) is 500W - 2000W, and the processing time is 200s - 2000s. In some embodiments, the processing power of the plasma treatment can be any value among 500, 600, 700, 800, 900, 1000, 1100, 1200, 1300, 1400, 1500, 1600, 1700, 1800, 1900, 2000W or the range value between any two values. The processing time can be any value among 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1100, 1200, 1300, 1400, 1500, 1600, 1700, 1800, 1900, 2000s or the range value between any two values.

[0045] In an embodiment of the present invention, a low-protein adsorption surface material prepared by the preparation method of the low-protein adsorption surface material is provided.

[0046] Photoinitiator 369, photoinitiator 907, and Irgacure 2959 are purchased from Ciba.

[0047] The room temperature is 10 - 30°C, including but not limited to 10, 15, 20, 25, 30°C.

[0048] Example 1

[0049] This example provides a preparation method of a low-protein adsorption surface material, including the following steps:

[0050] (1), Plasma treatment

[0051] Select an ep tube made of polypropylene as the material, use argon as the plasma gas, and under a processing power of 1000W, the processing time is 1000s.

[0052] (2), Preparation of the low-protein adsorption solution

[0053] The formula of the low-protein adsorption solution is as follows:

[0054] Substance A: 20g of polyvinyl alcohol;

[0055] Substance B: 10g of poly(ethylene glycol) methyl ether methacrylate;

[0056] Photoinitiator: 1g of photoinitiator 369;

[0057] 969g of deionized water.

[0058] According to the formula, put substance A, substance B monomer, and deionized water into a beaker, add a photoinitiator according to the formula, and stir with a magnetic electric stirrer at room temperature for 30 min to mix evenly.

[0059] (3), Coating preparation

[0060] Spray the low-protein adsorption solution in step (2) on the surface of the material to be treated in step (1), and the spraying conditions are 0.2 ml / min, 0.5 psi, and the spraying power is 4 w 。

[0061] (4), UV polymerization

[0062] Irradiate the material in step (3) at room temperature with a UV wavelength of 254 nm for a polymerization time of 30 min.

[0063] (5), Electron beam irradiation

[0064] Irradiate the material in step (4) with a high-energy electron beam at an irradiation dose of 30 kGy.

[0065] Example 2

[0066] This example provides a method for preparing a low-protein adsorption surface material, including the following steps:

[0067] (1), Plasma treatment

[0068] Select an ep tube made of polypropylene as the material, use argon as the plasma gas, and treat it at a treatment power of 1000 W for a treatment time of 1000 s.

[0069] (2), Preparation of low-protein adsorption solution

[0070] The formula of the low-protein adsorption solution is:

[0071] Substance A: 20 g of polyvinylpyrrolidone;

[0072] Substance B: 10 g of poly(ethylene glycol) methyl ether methacrylate;

[0073] Photoinitiator: 1 g of photoinitiator 369;

[0074] 969 g of deionized water.

[0075] According to the formula, put substance A, substance B monomer, and deionized water into a beaker, add a photoinitiator according to the formula, and stir with a magnetic electric stirrer at room temperature for 30 min to mix evenly.

[0076] (3), Coating preparation

[0077] Spray the low-protein adsorption solution in step (2) on the surface of the material to be treated in step (1). The spraying conditions are 0.2 ml / min, 0.5 psi, and the spraying power is 4 W. 。

[0078] (4), UV polymerization

[0079] Irradiate the material in step (3) at room temperature with a UV wavelength of 254 nm for a polymerization time of 30 min.

[0080] (5), Electron beam irradiation

[0081] Irradiate the material in step (4) with a high-energy electron beam at an irradiation dose of 30 kGy.

[0082] Example 3

[0083] This example provides a method for preparing a low-protein adsorption surface material, which includes the following steps:

[0084] (1), Plasma treatment

[0085] Select an EP tube made of polypropylene as the material, use argon as the plasma gas, and treat it at a treatment power of 1000 W for a treatment time of 1000 s.

[0086] (2), Preparation of low-protein adsorption solution

[0087] The formula of the low-protein adsorption solution is:

[0088] Substance A: 20 g of polyethylene glycol (molecular weight 3000);

[0089] Substance B: 10 g of poly(ethylene glycol) methyl ether methacrylate;

[0090] Photoinitiator: 1 g of photoinitiator 369;

[0091] 969 g of deionized water.

[0092] According to the formula, place Substance A, Substance B monomer, and deionized water in a beaker, add the photoinitiator according to the formula, and stir with a magnetic electric stirrer at room temperature for 30 min to mix evenly.

[0093] (3), Coating preparation

[0094] Spray the low-protein adsorption solution in step (2) on the surface of the material to be treated in step (1). The spraying conditions are 0.2 ml / min, 0.5 psi, and the spraying power is 4 W 。

[0095] (4), UV polymerization

[0096] The materials in step (3) are polymerized at room temperature with an ultraviolet wavelength of 254 nm for 30 min.

[0097] (5), Electron beam irradiation

[0098] The materials in step (4) are irradiated with high-energy electron beam with an irradiation dose of 30 kGy.

[0099] Example 4

[0100] The difference between this example and Example 2 is that the ultraviolet polymerization time in step (4) is 1 min; the irradiation dose in step (5) is 20 kGy.

[0101] Example 5

[0102] The difference between this example and Example 3 is that the molecular weight of polyethylene glycol is 5000.

[0103] Example 6

[0104] The difference between this example and Example 3 is that the molecular weight of polyethylene glycol is 30000.

[0105] Example 7

[0106] The difference between this example and Example 3 is that the molecular weight of polyethylene glycol is 2000.

[0107] Example 8

[0108] The difference between this example and Example 3 is that the molecular weight of polyethylene glycol is 35000.

[0109] Example 9

[0110] This example provides a preparation method of a low-protein adsorption surface material, including the following steps:

[0111] (1), Plasma treatment

[0112] Select an EP tube made of polypropylene as the material, use argon as the plasma gas, and treat it at a processing power of 1000 W for 1000 s.

[0113] (2), Preparation of low-protein adsorption solution

[0114] The formula of the low-protein adsorption solution is as follows:

[0115] Substance A: 5 g of polyethylene glycol (molecular weight of 3000);

[0116] Substance B: 25 g of polyethylene glycol diacrylate;

[0117] Photoinitiator: 0.25 g of photoinitiator 907;

[0118] The deionized water is 969.75 g.

[0119] According to the formula, put substance A, substance B monomer, and deionized water into a beaker, add the photoinitiator according to the formula, and stir with a magnetic electric stirrer at room temperature for 30 min to mix evenly.

[0120] (3) Coating preparation

[0121] Spray the low-protein adsorption solution in step (2) on the surface of the material to be treated in step (1). The spraying conditions are 0.2 ml / min, 0.5 psi, and the spraying power is 4 w. 。

[0122] (4) UV polymerization

[0123] Keep the material in step (3) at room temperature, with a UV wavelength of 365 nm and a polymerization time of 60 min.

[0124] (5) Electron beam irradiation

[0125] Irradiate the material in step (4) with high-energy electron beam, and the irradiation dose is 40 kGy.

[0126] Example 10

[0127] This example provides a method for preparing a low-protein adsorption surface material, which includes the following steps:

[0128] (1) Plasma treatment

[0129] Select an ep tube made of polypropylene as the material, use argon as the plasma gas, and treat it at a processing power of 1000 W for 1000 s.

[0130] (2) Preparation of low-protein adsorption solution

[0131] The formula of the low-protein adsorption solution is as follows:

[0132] Substance A: 50 g of polyvinyl alcohol;

[0133] Substance B: 2.5 g of polyethylene glycol diacrylate;

[0134] Photoinitiator: 2.5 g of photoinitiator 907;

[0135] The deionized water is 945 g.

[0136] According to the formula, put substance A, substance B monomer, and deionized water into a beaker, add the photoinitiator according to the formula, and stir with a magnetic electric stirrer at room temperature for 30 min to mix evenly.

[0137] (3) Coating preparation

[0138] Spray the low-protein adsorption solution in step (2) on the surface of the material to be treated in step (1), with the spraying conditions being 0.2 ml / min, 0.5 psi, and the spraying power being 4 w 。

[0139] (4), Ultraviolet polymerization

[0140] Keep the material in step (3) at room temperature, with an ultraviolet wavelength of 365 nm and a polymerization time of 10 min.

[0141] (5), Electron beam irradiation

[0142] Irradiate the material in step (4) with high-energy electron beam, and the irradiation dose is 25 kGy.

[0143] Comparative example 1

[0144] The difference between this example and Example 3 is that step (1) is omitted, polyethylene glycol in step (2) is replaced with glycerol in equal mass, the ultraviolet polymerization time in step (4) is 120 min; the irradiation dose in step (5) is 50 kGy.

[0145] Comparative example 2

[0146] The difference between this comparative example and Example 1 is that steps (1), (2) and (3) are omitted; the ultraviolet polymerization time in step (4) is 20 min; the irradiation dose in step (5) is 15 kGy.

[0147] Comparative example 3

[0148] The difference between this comparative example and Example 1 is that step (5) is omitted.

[0149] Experimental example 1 Performance test

[0150] 1) Add 200 ul of the blocking reagent ROTI Block to a black ELISA plate and block it at 37 °C for 2 h;

[0151] 2) Dilute the fluorescently labeled immunoglobulin IgG-FITC (20 mg / mL) with PBS in a gradient to a final concentration of 1 ug / mL and store it in a low-protein adsorption tube. Hereinafter, this solution is called the dilution solution.

[0152] 3) Let the dilution solution equilibrate at room temperature for about 20 min, and take 100 uL of the dilution solution and add it to the black ELISA plate that has been blocked in advance; Read the fluorescence value at A495 of the black ELISA plate with an enzyme-linked immunosorbent assay reader, and this value is the fluorescence value a0 of the initial concentration.

[0153] 4) Take out the low-protein adsorption container and add 500 μL of diluent to it. Place it at 4°C (seal it with a sealing film and wrap it with aluminum foil).

[0154] 5) After 24 h, take 100 μL of the adsorbed liquid and transfer it to a pre-blocked black ELISA plate to read the fluorescence value a at A495. n .

[0155] Protein recovery rate = a n / a0 × 100%.

[0156] Detect the low-protein adsorption containers obtained in the examples and comparative examples using the above method. The detection results are as follows:

[0157] Table 1

[0158]

[0159]

[0160] Table 2

[0161]

[0162] As can be seen from the above table, the protein recovery rates of the low-protein adsorption surface materials prepared in Examples 1-10 of the present invention are significantly higher than those of Comparative Examples 1-3. Among them:

[0163] When comparing Examples 1-10 with Comparative Example 1, it is found that the protein recovery rates of Examples 1-10 are significantly higher than those of Comparative Example 1, indicating that a high protein recovery rate can be obtained through the scheme of the present invention. In Comparative Example 1, since the step of first treating the surface of the material to be treated by low-temperature plasma is omitted, the surface energy of the material to be treated is affected, which in turn affects the spreading and attachment of Substance A, Substance B, and the photoinitiator on the material surface, and further affects the formation of a network structure on the material surface, ultimately affecting the low-protein adsorption performance of the material and resulting in a low protein recovery rate.

[0164] When comparing Examples 1-10 with Comparative Example 2, it is found that the protein recovery rates of Examples 1-10 are significantly higher than those of Comparative Example 2, indicating that a high protein recovery rate can be obtained through the scheme of the present invention. In Comparative Example 2, Substance A and Substance B are omitted. Even if the surface of the material to be treated is irradiated with ultraviolet light and high-energy electron beams, a network structure cannot be formed on the surface of the material to be treated, ultimately affecting the low-protein adsorption performance of the material and resulting in a low protein recovery rate.

[0165] Comparing Example 1 with Comparative Example 3, it was found that the protein recovery rate of Example 1 was significantly higher than that of Comparative Example 3, indicating that in the method of the present invention, the surface of the material to be treated is first treated by low-temperature plasma, and then Substance A, Substance B and a photoinitiator are added. Under ultraviolet irradiation, Substance A, Substance B and the photoinitiator form a 3D network hydrogel. By using high-energy electron beam irradiation, free radicals are generated in the Substance A molecules in the 3D network hydrogel, and the free radicals undergo self-crosslinking, resulting in a higher crosslinking density and forming a denser network structure, which is manifested as a lower swelling rate and higher strength, making the obtained material surface more suitable for long-term storage of protein and polypeptide samples, maintaining low adsorption and high protein recovery rate. In Comparative Example 3, the high-energy electron beam irradiation was omitted, and the 3D network hydrogel could no longer generate free radicals through Substance A molecules to form a denser network structure. Proteins and polypeptides were easily adsorbed on the material surface, and the protein recovery rate was significantly reduced.

[0166] Furthermore, through comparison in the examples, it was found that the protein recovery rates of Examples 3, 5, and 6 were significantly higher than those of other examples, indicating that when Substance A is selected as polyethylene glycol 3000 - 30000, the anti-protein adsorption performance of the material can be significantly improved, and the protein recovery rate can be significantly increased.

[0167] Experimental Example 2

[0168] Performed according to the method of Experimental Example 1, after placing at 4°C for 1 week, 4 weeks, 8 weeks, and 16 weeks in step 4) respectively, measure the fluorescence value a with step 5) n , and calculate the protein recovery rate. The results are shown in the following table.

[0169] Table 3. Protein recovery rate

[0170] Group 1 week 4 weeks 8 weeks 16 weeks Example 1 88.5％ 87.8％ 86.3％ 85.2％ Example 3 95.8％ 94.2％ 93.5％ 93.1％ Comparative Example 3 43.9％ 42.5％ 41.1％ 39.8％

[0171] As can be seen from the above table, after the samples were placed in the low-protein adsorption surface material for 1 week, 4 weeks, 8 weeks, and 16 weeks, the protein recovery rates of the low-protein adsorption surface materials prepared in Example 1 and Example 3 of the present invention were significantly higher than those of Comparative Example 3, indicating that the low-protein adsorption surface material of the present invention is suitable for long-term storage of protein and polypeptide samples, and still maintains low protein adsorption during long-term storage at low temperature. In addition, by comparing Example 1 and Example 3, it can be seen that as time prolongs, the decrease amplitude of the protein recovery rate in Example 3 is lower than that in Example 1, indicating that the low-protein adsorption surface material of Example 3 has better performance in maintaining low protein adsorption during long-term low-temperature storage.

[0172] Obviously, the above embodiments are merely examples given for clear illustration and not limitations on the implementation manners. For those of ordinary skill in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to enumerate all implementation manners here. And the obvious changes or modifications derived therefrom still fall within the protection scope of this invention.

Claims

1. A method for preparing a low protein adsorption surface material, characterized in that: include: (1) subjecting the surface of the material to be treated to low-temperature plasma treatment; (2) applying a low protein adsorption solution on the surface of the material to be treated; The low protein adsorption solution comprises substance A, substance B and a photoinitiator; the substance A is at least one of compounds containing at least one hydroxyl group; the substance B is a polyester polymer having a polyether segment; (3) subjecting the surface of the material to be treated coated in step (2) to ultraviolet polymerization; (4) The surface of the material to be treated obtained in step (3) is irradiated with a high-energy electron beam.

2. The method for preparing a low protein adsorption surface material according to claim 1, characterized in that: The irradiation dose of the high energy electron beam irradiation is 15-40KGy; And / or, the substance A is at least one of polyvinyl alcohol, polyvinyl pyrrolidone and polyethylene glycol, and the molecular weight of the polyethylene glycol is 2000-35000.

3. The method for preparing the low protein adsorption surface material according to claim 2, characterized in that: The molecular weight of the polyethylene glycol is 3,000 to 30,000.

4. The method for preparing the low protein adsorption surface material according to any one of claims 1 to 3, characterized in that: The polyester polymer of the polyether segment is at least one of polyethylene glycol diacrylate and poly(ethylene glycol) methyl ether methacrylate.

5. The method for preparing the low protein adsorption surface material according to any one of claims 1 to 4, characterized in that: The photoinitiator includes at least one of photoinitiator 369, photoinitiator 907, and Irgacure 2959.

6. The method for preparing the low protein adsorption surface material according to any one of claims 1 to 5, characterized in that: In the low protein adsorption solution, substance A accounts for 0.5-5 parts by weight, substance B accounts for 0.25-2.5 parts by weight, photoinitiator accounts for 0.025-0.25 parts by weight, and deionized water accounts for 92.25-99.25 parts by weight.

7. The method for preparing the low protein adsorption surface material according to any one of claims 1 to 6, characterized in that: The conditions of the ultraviolet polymerization are: ultraviolet polymerization is carried out at room temperature, the ultraviolet wavelength is 254nm or 365nm, and the polymerization time is 10min-60min.

8. The method for preparing the low protein adsorption surface material according to any one of claims 1 to 7, characterized in that: The material to be treated in step (1) is at least one selected from polystyrene, polypropylene, polycarbonate, and polyethylene terephthalate.

9. The method for preparing the low protein adsorption surface material according to any one of claims 1 to 8, characterized in that: The low-temperature plasma in step (1) is one or more of oxygen, nitrogen, and argon; And / or, the processing power of the plasma treatment in step (1) is 500W-2000W, and the processing time is 200s-2000s.

10. A low protein adsorption surface material prepared by the method for preparing a low protein adsorption surface material according to any one of claims 1 to 9.

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