Brassica rapa throat lozenges as well as preparation method and application thereof

Abstract

The invention provides brassica rapa throat lozenges and a preparation method and application thereof, and the brassica rapa throat lozenges comprise the following raw materials in parts by mass: 12-20 parts of brassica rapa juice; 6-10 parts of platycodon grandiflorum; 10 to 14 parts of scaphium scaphigerum; 8 to 12 parts of fructus momordicae; 8 to 12 parts of folium eriobotryae; 1-5 parts of licorice root; 8 to 12 parts of lily; 36-44 parts of brown rock sugar; 26 to 36 parts of corn syrup; 6 to 14 parts of lemon juice; and 126 to 134 parts of distilled water. According to the brassica rapa throat lozenge, the brown rock sugar, the corn syrup and the lemon juice are added, the taste and sense of the brassica rapa throat lozenge can be improved, and the brassica rapa throat lozenge prepared from the brassica rapa juice, the platycodon grandiflorum, the scaphium scaphigerum, the momordica grosvenori, the folium eriobotryae, the liquorice and the lily can have an obvious treatment effect on lung qi deficiency.

Description

Technical Field

[0001] The invention relates to the technical field of throat lozenges, and in particular to a Qiamagu throat lozenge and a preparation method and application thereof. Background Art

[0002] Deficiency of Lung Qi is a pathological change caused by the deficiency of lung qi and weakened function, which leads to poor breathing and dysfunction of the defense function. It is also a weak symptom manifested by the weakened lung function and the dysfunction of its main qi and defense function. Therefore, deficiency of lung qi refers to the state of weak lung qi, which can be caused by disease or sub-health state. Deficiency of lung qi syndrome can be caused by overwork, chronic cough, summer heat and serious illness, which damages lung qi, or spleen deficiency cannot ascend clear qi to the lung, water and grain essence and qi are insufficient, and the lung is not nourished, resulting in insufficient lung qi and weakened functional activity, forming deficiency of lung qi syndrome. The clinical manifestations of deficiency of lung qi syndrome are cough, wheezing, shortness of breath, clear and thin sputum, low and timid voice, fear of wind and spontaneous sweating, susceptibility to external pathogens, cough, fatigue, shortness of breath, pale complexion, fatigue, pale tongue with white fur, weak pulse, etc. Deficiency of lung qi syndrome is common in diseases such as chronic bronchitis, bronchiectasis, emphysema and cor pulmonale in Western medicine. Lung Qi deficiency syndrome is mostly caused by long-term consumption of lung Qi or insufficient lung Qi production. As the disease worsens, it may develop into spleen deficiency syndrome, kidney deficiency syndrome, lung and kidney deficiency, etc. Therefore, treatment should be started as early as possible in the lung Qi deficiency stage.

[0003] Qiamagu, also known as turnip and turnip, is the root of Brassicarapa L. in the cruciferous family. It is a popular food and medicine product of Xinjiang Uygur residents with a long history. Its medicinal part is the root, and the function and treatment of the seed turnip are similar to those of the root. Literature report [Wang Chengyang, Li Zegeng. Theoretical, clinical and experimental research on lung diseases based on lung qi deficiency syndrome [J]. Chinese Journal of Clinical Health Care, 2013, 16 (5): 552-555]. Its efficacy is recorded in the ancient "Book of Songs", "Er Ya", "Food Therapy Materia Medica" and "Compendium of Materia Medica". It has the effects of lowering blood sugar, opening the chest and relieving qi, strengthening the stomach and digestion, detoxifying, and improving immunity.

[0004] Among the existing technologies, the throat lozenges prepared by Chamagu have a poor taste and cannot treat lung qi deficiency. Summary of the invention

[0005] Based on this, the purpose of the present invention is to provide a Qiamagu throat lozenge and a preparation method and application thereof to solve the deficiencies in the above-mentioned prior art.

[0006] In a first aspect, the present invention provides a Qiamagu throat lozenge, wherein the raw materials of the Qiamagu throat lozenge are calculated by weight and include:

[0007] Chamagu juice, 12-20 servings;

[0008] Platycodon grandiflorum, 6-10 portions;

[0009] Semen Sterculiae Lychnophorae, 10 - 14 parts;

[0010] Fructus Momordicae Grosvenori, 8 - 12 parts;

[0011] Folium Eriobotryae Japonicae, 8 - 12 parts;

[0012] Radix Glycyrrhizae, 1 - 5 parts;

[0013] Bulbus Lilii, 8 - 12 parts;

[0014] Yellow rock sugar, 36 - 44 parts;

[0015] Corn syrup, 26 - 36 parts;

[0016] Lemon juice, 6 - 14 parts;

[0017] Distilled water, 126 - 134 parts.

[0018] Compared with the prior art, the beneficial effects of the present invention are as follows: By adding yellow rock sugar, corn syrup and lemon juice, the taste and sensory properties of the Cichorium intybus throat lozenge can be improved, and the Cichorium intybus throat lozenge prepared from Cichorium intybus juice, Platycodon grandiflorum, Semen Sterculiae Lychnophorae, Fructus Momordicae Grosvenori, Folium Eriobotryae Japonicae, Radix Glycyrrhizae and Bulbus Lilii can significantly treat lung qi deficiency.

[0019] Further, the mass ratio of the Cichorium intybus juice to the distilled water is 8:65.

[0020] Further, the pH value of the distilled water is 6.6.

[0021] In a second aspect, the present invention also provides a method for preparing a Cichorium intybus throat lozenge for preparing the above-mentioned Cichorium intybus throat lozenge, and the method includes:

[0022] Performing a first mixing and boiling of the Cichorium intybus juice with Platycodon grandiflorum, Semen Sterculiae Lychnophorae, Fructus Momordicae Grosvenori, Folium Eriobotryae Japonicae, Radix Glycyrrhizae and Bulbus Lilii to obtain a mixed liquid;

[0023] Performing a second mixing and boiling of the yellow rock sugar, corn syrup, distilled water and lemon juice until concentrated, and adding the mixed liquid when the temperature is reduced to a first preset temperature, and continuously stirring and heating to a second preset temperature to obtain a syrup;

[0024] Pouring the syrup into a mold for cooling and shaping to obtain the Cichorium intybus throat lozenge.

[0025] Further, the first preset temperature is 124 °C and the second preset temperature is 140 °C.

[0026] Further, before the step of performing a first mixing and boiling of the Cichorium intybus juice with Platycodon grandiflorum, Semen Sterculiae Lychnophorae, Fructus Momordicae Grosvenori, Folium Eriobotryae Japonicae, Radix Glycyrrhizae and Bulbus Lilii to obtain a mixed liquid, the method further includes:

[0027] Wash, peel and chop the Cichorium glandulosum Boiss. in sequence, and juice the chopped Cichorium glandulosum Boiss. to obtain Cichorium glandulosum Boiss. puree;

[0028] Filter the Cichorium glandulosum Boiss. puree with a single layer of gauze to obtain the Cichorium glandulosum Boiss. juice.

[0029] Furthermore, the temperature of the first mixed boiling and the second mixed boiling is 136 - 144 °C.

[0030] Thirdly, the present invention also provides an application of the Cichorium glandulosum Boiss. throat lozenge as described above in the preparation of a medicament for treating lung qi deficiency. Description of the Drawings

[0031] Figure 1 It is a physical diagram of the Cichorium glandulosum Boiss. throat lozenge in the embodiment of the present invention;

[0032] Figure 2 It is the influence of the addition amount of Cichorium glandulosum Boiss. juice on the sensory score of the Cichorium glandulosum Boiss. throat lozenge in the embodiment of the present invention;

[0033] Figure 3 It is the influence of the addition amount of yellow rock sugar on the sensory score of the Cichorium glandulosum Boiss. throat lozenge in the embodiment of the present invention;

[0034] Figure 4 It is the influence of the addition amount of corn syrup on the sensory score of the Cichorium glandulosum Boiss. throat lozenge in the embodiment of the present invention;

[0035] Figure 5 It is the influence of the addition amount of water on the sensory score of the Cichorium glandulosum Boiss. throat lozenge in the embodiment of the present invention;

[0036] Figure 6 It is the influence of different firing temperatures of corn syrup on the sensory score of the Cichorium glandulosum Boiss. throat lozenge in the embodiment of the present invention;

[0037] Figure 7 It is the influence of the addition amount of lemon juice on the sensory score of the Cichorium glandulosum Boiss. throat lozenge in the embodiment of the present invention;

[0038] Figure 8 It is the response surface diagram and contour diagram of the influence of the interaction between yellow rock sugar and fresh Cichorium glandulosum Boiss. juice on the sensory score of the test throat lozenge in the present invention;

[0039] Figure 9 It is the response surface diagram and contour diagram of the influence of the interaction between fresh Cichorium glandulosum Boiss. juice and boiling temperature on the sensory score of the test throat lozenge in the present invention;

[0040] Figure 10 It is a schematic diagram of the influence of the Cichorium glandulosum Boiss. throat lozenge on the food intake of mice;

[0041] Figure 11Schematic diagram of the effect of Cichorium glandulosum lozenges on the body weight of mice and the effect of Cichorium glandulosum lozenges on the water content of mouse feces;

[0042] Figure 12 Schematic diagram of the effect of Cichorium glandulosum lozenges on tumor necrosis factor α (TNF-α) and interleukin 1β (IL-1β) in mice and the effect of Cichorium glandulosum lozenges on the water intake of mice;

[0043] Figure 13 Effect of Cichorium glandulosum lozenges on the organ index of mice.

[0044] The following specific embodiments will further illustrate the present invention in conjunction with the above-mentioned drawings. Specific Embodiment

[0045] To facilitate the understanding of the present invention, the present invention will be described more comprehensively with reference to the relevant drawings. Several embodiments of the present invention are given in the drawings. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, these embodiments are provided to make the disclosure of the present invention more thorough and comprehensive.

[0046] It should be noted that when an element is referred to as "fixed on" another element, it can be directly on the other element or there may also be an intermediate element. When an element is considered to be "connected" to another element, it can be directly connected to the other element or there may be an intermediate element at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only.

[0047] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the technical field to which the present invention belongs. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more of the related listed items.

[0048] Embodiment 1

[0049] The first embodiment of the present invention provides a Cichorium glandulosum lozenge. The raw materials of the Cichorium glandulosum lozenge, by mass, include 16 parts of Cichorium glandulosum juice, 8 parts of Platycodon grandiflorum, 12 parts of Sterculia lychnophora, 10 parts of Momordica grosvenori, 10 parts of Eriobotrya japonica leaves, 3 parts of Glycyrrhiza uralensis, 10 parts of Lilium brownii, 40 parts of yellow rock sugar, 32 parts of corn syrup, 10 parts of lemon juice, and 130 parts of distilled water.

[0050] It should be noted that the mass ratio of Cichorium glandulosum juice to distilled water is 8:65, and the pH value of distilled water is 6.6.

[0051] In specific implementation, first wash, peel and chop the Cichorium glandulosum Boiss. var. sativum, and then juice the chopped Cichorium glandulosum Boiss. var. sativum to obtain Cichorium glandulosum Boiss. var. sativum puree; filter the Cichorium glandulosum Boiss. var. sativum puree with a single-layer gauze to obtain the Cichorium glandulosum Boiss. var. sativum juice. Thus, fresh Cichorium glandulosum Boiss. var. sativum juice can be obtained.

[0052] The process method for preparing the throat lozenges includes steps S1 to S3:

[0053] S1, Mix 16 g of Cichorium glandulosum Boiss. var. sativum juice with 8 g of Platycodon grandiflorum, 12 g of Sterculia lychnophora, 10 g of Momordica grosvenori, 10 g of Eriobotrya japonica, 3 g of Glycyrrhiza uralensis and 10 g of Lilium brownii var. viridulum for the first-time mixed boiling to obtain a mixed liquid;

[0054] S2, Mix 40 g of yellow rock sugar, 32 g of corn syrup, 130 g of distilled water and 10 g of lemon juice for the second-time mixed boiling until concentrated, add the mixed liquid when the temperature is reduced to the first preset temperature, and continuously stir and heat up to the second preset temperature to obtain syrup;

[0055] S3, Pour the syrup into a mold for cooling and forming to obtain Cichorium glandulosum Boiss. var. sativum throat lozenges.

[0056] The obtained throat lozenges are specifically as Figure 1 shown.

[0057] In this embodiment, the temperatures for the first-time mixed boiling and the second-time mixed boiling are both 140 °C, the first preset temperature is 124 °C, and the second preset temperature is 140 °C.

[0058] Embodiment 2

[0059] The difference between the second embodiment and the first embodiment of the present invention is that:

[0060] The Cichorium glandulosum Boiss. var. sativum juice is 12 g, the yellow rock sugar is 36 g, the corn syrup is 28 g, the distilled water is 126 g, and the lemon juice is 6 g. The temperatures for the first-time mixed boiling and the second-time mixed boiling are both 136 °C.

[0061] Embodiment 3

[0062] The difference between the third embodiment and the above embodiments of the present invention is that:

[0063] The Cichorium glandulosum Boiss. var. sativum juice is 14 g, the yellow rock sugar is 38 g, the corn syrup is 30 g, the distilled water is 128 g, and the lemon juice is 8 g. The temperatures for the first-time mixed boiling and the second-time mixed boiling are both 148 °C.

[0064] Embodiment 4

[0065] The difference between the fourth embodiment and the above embodiments of the present invention is that:

[0066] The amount of Cichorium glandulosum juice is 18 g, the amount of yellow rock sugar is 42 g, the amount of corn syrup is 34 g, the amount of distilled water is 132 g, and the amount of lemon juice is 12 g. The temperature for both the first and second mixed boiling processes is 142 °C.

[0067] Example 5

[0068] The difference between the fifth embodiment of the present invention and the above embodiments lies in:

[0069] The amount of Cichorium glandulosum juice is 20 g, the amount of yellow rock sugar is 44 g, the amount of corn syrup is 36 g, the amount of distilled water is 134 g, and the amount of lemon juice is 14 g. The temperature for both the first and second mixed boiling processes is 144 °C.

[0070] On the basis of single-factor experiments, factors that have a greater impact on the sensory score of the experimental throat lozenges are selected, namely the addition amount of fresh Cichorium glandulosum juice, the addition amount of yellow rock sugar, and the firing temperature for the Box-Benhnken response surface experiment. The factors and levels are shown in Table 1:

[0071] Table 1 Response surface factor level table

[0072]

[0073] On the basis of referring to GB / T23823-2009 "Classification of Candies", a sensory scoring standard for Cichorium glandulosum throat lozenges is designed, as shown in Table 2. The sensory scoring is judged by 8 trained evaluators from four aspects: the appearance, smell and taste, tissue state, and color of the experimental throat lozenges. Table 2:

[0074] Table 2 Sensory scoring table for Cichorium glandulosum throat lozenges

[0075]

[0076] The influence of the addition amount of fresh Cichorium glandulosum juice on the sensory score of the experimental throat lozenges can be seen in Figure 2 , from Figure 2 it can be known that the sensory score of the experimental throat lozenges first increases and then decreases with the increase of the addition amount of fresh Cichorium glandulosum juice. When the addition amount of fresh Cichorium glandulosum juice in the experimental throat lozenges is 16 g, the sensory score is the highest at 80. It may be because when the addition amount of fresh Cichorium glandulosum juice increases, the taste of Cichorium glandulosum is stronger and it is more difficult for the hard candy to form; when the addition amount of fresh Cichorium glandulosum juice decreases, the taste of Cichorium glandulosum is lighter. Considering comprehensively, the most suitable addition amount of fresh Cichorium glandulosum juice is 16 g.

[0077] The influence of the addition amount of yellow rock sugar on the sensory score of the experimental throat lozenges can be seen in Figure 3 , from Figure 3It can be seen that the sensory score of the experimental throat lozenges first increases and then decreases with the addition amount of yellow rock sugar. When the addition amount of yellow rock sugar in the experimental throat lozenges is 40 g, the sensory score is the highest at 80. This may be because when the addition amount of yellow rock sugar increases, the sweetness is greater; when the addition amount of yellow rock sugar decreases, the acidity is greater.

[0078] The influence of the addition amount of corn syrup on the sensory score of the experimental throat lozenges is shown in Figure 4 , from Figure 4 It can be seen that the sensory score of the experimental throat lozenges first increases and then decreases with the addition amount of corn syrup. When the addition amount of corn syrup in the experimental throat lozenges is 32 g, the sensory score is the highest at 80. This may be because when the addition amount of corn syrup increases, the ductility of the throat lozenges is better and the hardness is worse; when the addition amount of corn syrup decreases, the surface of the throat lozenges is relatively rough, affecting the taste.

[0079] The influence of the addition amount of water on the sensory score of the experimental throat lozenges is shown in Figure 5 , from Figure 5 It can be seen that the sensory score of the experimental throat lozenges first increases and then decreases with the addition amount of water. When the addition amount of water in the experimental throat lozenges is 130 g, the sensory score is the highest at 80. This may be because when the addition amount of water increases, the water content of the throat lozenges is slightly larger and the surface is relatively sticky; when the addition amount of water decreases, it is more difficult for the yellow rock sugar to melt, and the texture of the throat lozenges has a granular feeling.

[0080] The influence of the firing temperature on the sensory score of the experimental throat lozenges is shown in Figure 6 , from Figure 6 It can be seen that the sensory score of the experimental throat lozenges first increases and then decreases with the increase of the firing temperature. When the firing temperature of the experimental throat lozenges is 140 °C, the sensory score is the highest at 80. This may be because when the firing temperature increases, the syrup is prone to burning and the throat lozenges have a burnt smell; when the firing temperature decreases, the hardness of the throat lozenges is smaller and the surface is sticky.

[0081] The influence of the addition amount of lemon juice on the sensory score of the experimental throat lozenges is shown in Figure 7 , from Figure 7 It can be seen that the sensory score of the experimental throat lozenges first increases and then decreases with the addition amount of lemon juice. When the addition amount of lemon juice in the experimental throat lozenges is 10 g, the sensory score is the highest at 80. This may be because when the addition amount of lemon juice increases, the throat lozenges are more sour; when the addition amount of lemon juice decreases, the throat lozenges are sweeter.

[0082] Using the Box-Behnken experimental design in Design-Expert software, with the sensory score as the response value, the Box-Behnken experimental design was carried out, and the results are shown in Table 3. The regression fitting was performed on the results in Table 3, and the multiple regression equation of the sensory score with the addition amount of yellow rock sugar (A), the addition amount of fresh Qiemagu juice (B), and the boiling temperature (C) is: Y = 85.40 + 0.375×A - 1×B - 0.125×C + 1.25×AB + 0.5×AC + 0.25×BC - 1.2×A² - 1.95×B² - 4.7×C². The results of the variance analysis are shown in Table 4.

[0083] Table 3

[0084]

[0085] Table 4

[0086]

[0087]

[0088] It should be noted that the correlation coefficient R of the model 2 = 0.9529, the adjusted determination coefficient R 2 adj = 0.8923, P < 0.05, indicating that the model or the investigated factors have a significant influence, and P < 0.01 indicates that the model or the reference factors have a highly significant influence.

[0089] According to the regression equation, the response surface diagram and the contour diagram were drawn to analyze the influence of the interaction between any two of the three factors of yellow rock sugar, fresh Qiemagu juice, and low-gluten flour on the response value. The significance was judged from the 3D diagram according to the steepness of the formed plane slope: the steeper the slope, the more significant the interaction of the factors; if the slope is gentle, the interaction is not significant. The contour diagram indicates that within the same elliptical area, the ellipse indicates a significant interaction, and conversely, being close to a circle indicates that the interaction is not obvious and can be ignored. Figure 8 and Figure 9 are the response surface diagram and the contour diagram of the interaction between the factors.

[0090] From Figure 8 it can be seen that Figure 8 in which A represents fresh Qiemagu juice, B represents yellow rock sugar. When the boiling temperature is 140 °C, the response surface has a certain slope and the contour line is an ellipse, indicating that the interaction between the addition amounts of yellow rock sugar and Qiemagu juice has an obvious influence on the sensory score. It shows that the influence on the sensory score of the test product is that the addition amount of Qiemagu juice > the addition amount of yellow rock sugar.

[0091] From Figure 9 it can be seen that Figure 9In it, A represents fresh Cichorium glandulosum juice, and C represents the boiling temperature. When the addition amount of Cichorium glandulosum juice is 16 g, the response surface has a certain slope and the contour line is elliptical, indicating that the firing temperature and the addition amount of yellow rock sugar have an impact on the sensory score interaction but not significantly, indicating that the factor with a greater impact on the sensory score of the experimental product is the addition amount of yellow rock sugar > the firing temperature.

[0092] It can be seen from Figure 3 that when the addition amount of yellow rock sugar is 40 g, the response surface has a certain slope, which is Figure 8 , Figure 9 smaller than that, and the contour line is elliptical, indicating that the firing temperature and the addition amount of Cichorium glandulosum juice have an impact on the sensory score interaction but not significantly, indicating that the factor with a greater impact on the sensory score of the experimental product is the addition amount of Cichorium glandulosum juice > the firing temperature.

[0093] Example Six

[0094] The sixth embodiment of the present invention provides an application of Cichorium glandulosum lozenges in the preparation of drugs for treating lung qi deficiency and a bioactivity study of Cichorium glandulosum lozenges.

[0095] The purpose is to explore the effect of Cichorium glandulosum lozenges on improving lung qi deficiency in mice. Methods: NK mice were randomly divided into a blank group and a control group, with 7 mice in the blank group and 28 mice in the control group. Except for the blank group, the control group was used to replicate the lung qi deficiency model by the improved sawdust smoking method. After successful modeling, the modeled rats were divided into a model group, a high-dose group of Cichorium glandulosum lozenges (0.938 g / kg), a low-dose group of Cichorium glandulosum lozenges (0.469 g / kg), and a positive control group (dexamethasone tablets, 1 mg / kg, with water as the solvent), with 7 mice in each group, ensuring sufficient water and feed. Starting from 7 days after modeling, except for the blank group and the model group, the mice in the other drug groups were intragastrically administered the corresponding medicinal liquid at 0.2 ml / 10 g, and the mice in the blank group and the model group were intragastrically administered an equal volume of distilled water, once a day, for 28 consecutive days. The body weight, food intake, water intake, fecal water content, anal temperature, organ weight, nitric oxide content, tumor necrosis factor a (TNF-A), and interleukin 1β (IL-1β) content of the mice in each group were detected, and the organ index was calculated.

[0096] The main instruments include: mouse interleukin-1β (IL-1β) ELISA detection kit (Quanzhou Ruixin Biotechnology Co., Ltd., RX203063M), mouse tumor necrosis factor α (TNF-α) ELISA detection kit (Quanzhou Ruixin Biotechnology Co., Ltd., RX202412M), nitric oxide (NO) content determination kit (Nanjing Jiancheng Bioengineering Institute), ultrasonic oscillator (Kunshan, KQ3200E), rotary evaporator (Zhengzhou, R-1001VN), constant temperature magnetic stirrer (DF-101S), desktop refrigerated high-speed centrifuge (GENIUS, 16K-R), electronic analytical balance (ES-E2208), cylindrical fumigation box (height 21.8 cm, diameter 23.5 cm).

[0097] The main drugs and reagents include: Chamagu freeze-dried tablets, dexamethasone tablets (Nanguo Pharmaceutical Co., Ltd., Tianjin, China, Sinopharm H20043915, specification 30 mg × 50 tablets, batch number: 1902001), petroleum ether and ethanol (Xilong Chemical Co., Ltd.), chloroform (Sinopharm Chemical Reagent Co., Ltd.), and Jinsheng brand cigarettes (tar content 10 mg, smoke nicotine content 1.0 mg).

[0098] The experimental animals were 35 Beijing KM mice aged 4 weeks and weighing 20-25g male mice, which were adaptively raised under normal feeding conditions for 7 days with free access to water and feed.

[0099] Establishment of mouse model Grouping and sample collection: After adaptive feeding, the mice were randomly divided into 7 groups, 7 in each group, to ensure sufficient water and feed: blank group, model group, high-dose Qiamagu lozenges (12g / kg) group, low-dose Qiamagu lozenges (6g / kg) group and positive control group (dexamethasone tablets, 1mg / kg, with water as solvent). Starting from 7 days after modeling, except for the blank group and model group, the other groups were given the above-mentioned drugs 0.2ml / 10g by gavage, and the blank group and model group were gavaged with an equal amount of distilled water, once a day, for 28 days. Except for the blank group, the other mice were smoked in a cylindrical fumigation box with a height of 21.8cm and a diameter of 23.5cm for 20 minutes every day, 2-3 cigarettes each time, twice a day, and the interval between the two times was more than 4h, and smoked three times in the fourth week. After resting for 10 minutes after each fumigation, they were placed in a 16℃ air-conditioned room with cold wind for 30 groups. The blank group mice were also placed in the fumigation box, but not smoked. After the last gavage on the 28th, the mice were fasted for 12 hours and had free access to water. Then, the eyeballs were removed to collect blood, and all mice were killed by dislocating the neck. The organs were separated and frozen at -80°C for later use. A portion of the left lung of all mice was taken and stored in 10% paraformaldehyde solution for histopathological evaluation. Whole blood was taken and placed at room temperature for 1 hour, centrifuged at 3000r / min for 10 minutes at 4°C in a low-temperature high-speed centrifuge to obtain serum, which was frozen at -20°C for later use.

[0100] Detection of water content in mouse feces: During the drug administration period, the feces of rats were cleaned every day; after the last drug administration, the mice were placed in a metabolic cage, and the fresh feces they excreted were collected and weighed on an analytical balance (i.e., the mass of fresh feces); subsequently, the feces were dried for 5 h (at 110 °C), weighed again after cooling (i.e., the mass of dry feces), and the water content of the feces was calculated according to the following formula: Water content = (Mass of fresh feces - Mass of dry feces) / Mass of fresh feces × 100%.

[0101] Statistical method: All data are expressed as (x ± s), and the data statistics are compared using the t-test. The index is calculated using the following formula: Thymus index (spleen index) of the group = 100 × Thymus weight (spleen weight) / Body weight (100 g).

[0102] Effect of Cichorium glandulosum Boiss. var. album Regel throat lozenges on the food intake of mice: According to statistical analysis, when Cichorium glandulosum Boiss. var. album Regel throat lozenges were administered for 7 d, compared with the blank group, the positive control group was significantly decreased (P < 0.05), and the high-dose group of Cichorium glandulosum Boiss. var. album Regel throat lozenges was significantly decreased (P < 0.01). When Cichorium glandulosum Boiss. var. album Regel throat lozenges were administered for 14 d, compared with the blank group, the low-dose group of Cichorium glandulosum Boiss. var. album Regel throat lozenges was significantly decreased (P < 0.05), the model group and the positive control group were significantly decreased (P < 0.01), and the high-dose group of Cichorium glandulosum Boiss. var. album Regel throat lozenges was significantly decreased (P < 0.001); compared with the model group, the high-dose group of throat lozenges was significantly decreased (P < 0.05). When Cichorium glandulosum Boiss. var. album Regel throat lozenges were administered for 21 d, compared with the blank group, the model group, the low-dose group of Cichorium glandulosum Boiss. var. album Regel throat lozenges, and the positive control group were all significantly decreased (P < 0.05), and the high-dose group of Cichorium glandulosum Boiss. var. album Regel throat lozenges was significantly decreased more severely (P < 0.01). When Cichorium glandulosum Boiss. var. album Regel throat lozenges were administered for 28 d, compared with the blank group, the model group, the high-dose group of Cichorium glandulosum Boiss. var. album Regel throat lozenges, and the positive control group were all significantly decreased (P < 0.05), and the low-dose group of Cichorium glandulosum Boiss. var. album Regel throat lozenges was significantly decreased more severely (P < 0.01). According to traditional Chinese medicine theory, deficiency of lung qi refers to insufficient lung function, and people with qi deficiency constitution have insufficient vital qi and should not overeat to avoid consuming and dispersing qi. The high and low dose groups of Cichorium glandulosum Boiss. var. album Regel throat lozenges improve the disease of deficiency of lung qi, and the positive control group also has this effect. As Figure 10 in (a) to (d).

[0103] Effect of Qiemagugu lozenges on the body weight of mice: When Qiemagugu lozenges were administered for 7 days, compared with the blank group, the body weight of the model group decreased significantly (p < 0.05). The high-dose Qiemagugu lozenge group and its positive control group both showed significant decreases. Among them, the high-dose Qiemagugu lozenge group had a more significant decrease (p < 0.01), while the positive control group had a significant decrease (p < 0.05). When Qiemagugu lozenges were administered for 14 days, compared with the blank group, the body weight of the model group still decreased significantly (p < 0.05). The high-dose Qiemagugu lozenge group had a significant decrease (p < 0.01), while the positive control group had a significant decrease (p < 0.001). Compared with the model group, the positive control group had a significant decrease (p < 0.05). When Qiemagugu lozenges were administered for 21 days, compared with the blank group, the significant decrease in the body weight of the model group was aggravated (p < 0.01). The high-dose Qiemagugu lozenge group and its positive control group both showed significant decreases (p < 0.001). Compared with the model group, both the high-dose Qiemagugu lozenge group and its positive control group had significant decreases (p < 0.01). When Qiemagugu lozenges were administered for 28 days, compared with the blank group, the body weight of the model group decreased significantly (p < 0.01). The high-dose Qiemagugu lozenge group and its positive control group still both showed significant decreases (p < 0.001). Compared with the model group, both the high-dose Qiemagugu lozenge group and its positive control group had significant decreases. Among them, the positive control group had a more significant decrease (p < 0.001), while the high-dose Qiemagugu lozenge group had a significant decrease (p < 0.05). There was no significant difference in the above indicators compared with the low-dose Qiemagugu lozenge group (P > 0.05). Compared with the model group, the high-dose Qiemagugu lozenge group could improve the activity state of mice with the syndrome of lung qi deficiency, and the positive drug group also had such an effect. However, the low-dose Qiemagugu lozenge group did not show any effect on body weight. The results are as Figure 11 shown in (e) to (i) of

[0104] Effect of Qiemagugu lozenges on the water content of mouse feces: According to statistical analysis, compared with the blank group, the high-dose Qiemagugu lozenge group had a significant decrease (p < 0.05), the low-dose Qiemagugu lozenge group had a significant decrease (P < 0.01), and the positive control group had a significant decrease (P < 0.001). Compared with the model group, both the low-dose and high-dose Qiemagugu lozenge groups had significant decreases (P < 0.01), and the positive control group had a more significant decrease (P < 0.001). As shown in Figure 11 (g) of

[0105] Effect of Qiemagugu throat lozenges on tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in mice: After statistical analysis, compared with the blank group, TNF-α and IL-1β in the model group were significantly increased. Among them, p < 0.01 for TNF-α in the model group of mice, and p < 0.05 for IL-1β in the model group of mice. TNF-α in the low-dose group of Qiemagugu throat lozenges was significantly increased (p < 0.05); compared with the model group, TNF-α and IL-1β in the high-dose group of Qiemagugu throat lozenges were significantly decreased (p < 0.05), and IL-1β in the positive control group was significantly decreased (p < 0.05). It shows that the high-dose group of Qiemagugu throat lozenges and its positive control group can improve inflammation, enhance immune ability, and treat the disease of lung qi deficiency. Specifically as Figure 12 in (k) to (l) of

[0106] Effect of Qiemagugu throat lozenges on water intake of mice: When Qiemagugu throat lozenges were given for 7 days, compared with the blank group, the water intake in the model group was significantly decreased, p < 0.05. When Qiemagugu throat lozenges were given for 14 days, compared with the blank group, the low-dose group of Qiemagugu throat lozenges and its positive control group were both significantly decreased and p < 0.05, while the high-dose group of Qiemagugu throat lozenges was significantly more decreased and p < 0.01; compared with the model group, the high-dose group of Qiemagugu throat lozenges was significantly decreased, p < 0.05. When Qiemagugu throat lozenges were given for 21 days, compared with the blank group, the model group and its positive control group were both significantly decreased and p < 0.01, while the low-dose group of Qiemagugu throat lozenges and the high-low-dose group of Qiemagugu throat lozenges were significantly more decreased and p < 0.001; compared with the model group, the high-dose group of Qiemagugu throat lozenges was significantly decreased, p < 0.05. When Qiemagugu throat lozenges were given for 28 days, compared with the blank group, the model group and its positive control group were both significantly decreased and p < 0.05, while the low-dose group of Qiemagugu throat lozenges and the high-low-dose group of Qiemagugu throat lozenges were significantly more decreased and p < 0.001; compared with the model group, the low-dose group of Qiemagugu throat lozenges and the high-low-dose group of Qiemagugu throat lozenges were significantly decreased, p < 0.001. In terms of water intake, compared with the model group, there was no significance in the positive control group from 7 days to 28 days. For lung qi deficiency, the qi in the body is insufficient, and the transportation of water and fluid in the body requires the movement of qi, so the water and fluid are not easily transported. The high-low-dose groups of Qiemagugu throat lozenges can improve the disease of lung qi deficiency, and the positive control group also has this effect. As Figure 12 in (m) to figure (p) of

[0107] Effect of Qiemagu lozenges on visceral indices of mice: After statistical analysis, for the kidney index, compared with the blank group, the high-dose group of Qiemagu lozenges showed a significant decrease compared with the blank group and the model group, p < 0.01. For the spleen index, compared with the blank group, the high-dose group and the low-dose group of Qiemagu lozenges showed a significant decrease, p < 0.05, and the positive control group showed a more significant decrease, p < 0.001; compared with the model group, the positive control group showed a significant decrease, p < 0.001. For the liver index, compared with the blank group, the model group, the low-dose group and the high-dose group of Qiemagu lozenges and its positive control group all showed a significant decrease, among which p < 0.05 for the model group, p < 0.01 for the positive control group, and p < 0.001 for the high-dose group and the low-dose group of Qiemagu lozenges. It shows that the high-dose and low-dose groups of Qiemagu lozenges can improve the disease of lung qi deficiency and enhance the immune ability, and the positive control group also has this effect. Among them, the low-dose group of Qiemagu lozenges only showed this effect in the liver index. As Figure 13 from (q) to (s) in

[0108] In summary, the Qiemagu lozenges in the above embodiments of the present invention can improve the taste and sensory properties of the Qiemagu lozenges by adding yellow rock sugar, corn syrup and lemon juice, and the Qiemagu lozenges prepared from Qiemagu juice, platycodon root, boat-fruited sterculia seed, monk fruit, eriobotrya japonica leaf, licorice root and lily can play an obvious therapeutic role in lung qi deficiency.

[0109] In the description of this specification, the description with reference to terms such as "one embodiment", "some embodiments", "example", "specific example", or "some examples" means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in a suitable manner in any one or more embodiments or examples.

[0110] The above embodiments only represent several implementation manners of the present invention, and the description is relatively specific and detailed, but it should not be construed as a limitation on the scope of the patent of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present invention, several modifications and improvements can still be made, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention should be subject to the appended claims.

Claims

1. A kind of Cichorium glandulosum lozenge, characterized in that, The raw materials of the said Cichorium glandulosum lozenges, by mass parts, include: Cichorium glandulosum juice, 12 - 20 parts; Platycodon grandiflorum, 6 - 10 parts; Sterculia lychnophora, 10 - 14 parts; Momordica grosvenori, 8 - 12 parts; Eriobotrya japonica leaves, 8 - 12 parts; Glycyrrhiza glabra, 1 - 5 parts; Lilium brownii, 8 - 12 parts; Yellow rock sugar, 36 - 44 parts; Corn syrup, 26 - 36 parts; Lemon juice, 6 - 14 parts; Distilled water, 126 - 134 parts.

2. The Qiemagugu throat lozenge according to claim 1, wherein The mass ratio of the said Cichorium glandulosum juice to the said distilled water is 8:

65.

3. The Qiemagugu throat lozenge according to claim 1, characterized in that, The pH value of the said distilled water is 6.

6.

4. A preparation method of the Cichorium glandulosum lozenge, which is used to prepare the Cichorium glandulosum lozenge described in any one of claims 1 to 3, and is characterized in that, The said method includes: First, mix and boil the Cichorium glandulosum juice with Platycodon grandiflorum, Sterculia lychnophora, Momordica grosvenori, Eriobotrya japonica leaves, Glycyrrhiza glabra and Lilium brownii to obtain a mixed liquid; Second, mix and boil the yellow rock sugar, corn syrup, distilled water and lemon juice until concentrated, and when cooled to the first preset temperature, add the said mixed liquid and continuously stir and heat up to the second preset temperature to obtain a syrup; Pour the said syrup into a mold for cooling and shaping to obtain the Cichorium glandulosum lozenges.

5. The preparation method of the Qiemagugu throat lozenge according to claim 4, characterized in that, The first preset temperature is 124°C, and the second preset temperature is 140°C.

6. The preparation method of the Qiemagugu throat lozenge according to claim 4, characterized in that, Before the step of first mixing and boiling the Cichorium glandulosum juice with Platycodon grandiflorum, Sterculia lychnophora, Momordica grosvenori, Eriobotrya japonica leaves, Glycyrrhiza glabra and Lilium brownii to obtain a mixed liquid, the said method further includes: Wash, peel and chop the Cichorium glandulosum in sequence, and juice the chopped Cichorium glandulosum to obtain Cichorium glandulosum paste; Filter the said Cichorium glandulosum paste with a single - layer gauze to obtain the said Cichorium glandulosum juice.

7. The preparation method of the Qiemagugu throat lozenge according to claim 4, characterized in that, The temperature of the first mixing and boiling and the second mixing and boiling is 136 - 144°C.

8. Use of the Cichorium glandulosum lozenges according to claim 1 in the preparation of a medicament for treating deficiency of lung qi.

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