Non-surgical and non-filling wrinkle-removing and anti-aging skin-generating oil preparation for external use as well as preparation method and application of skin-generating oil preparation

Abstract

The invention belongs to the technical field of medicines, and particularly discloses a non-surgical and non-filling wrinkle-removing and anti-aging skin-generating oil preparation for external use as well as a preparation method and application of the non-surgical and non-filling wrinkle-removing and anti-aging skin-generating oil preparation. The preparation method of the external skin-generating oil preparation comprises the following steps: (1) preparing freeze-dried powder of a skin-generating oil alcohol extract: respectively taking dry powder of gynura procumbens, pericarpium citri reticulatae, astragalus membranaceus, angelica sinensis, frankincense, herba epimedii, scutellaria baicalensis and golden cypress and borneol according to a certain weight part ratio, adding the dry powder and borneol into a container, adding an ethanol solution with the volume fraction of 40-90% into the container, soaking, carrying out flash extraction, and filtering to obtain the freeze-dried powder of the skin-generating oil alcohol extract; and concentrating the filtrate, and freeze-drying. (2) preparing a skin-generating oil preparation: dissolving the skin-generating oil alcohol extract freeze-dried powder and Span 60 in edible vegetable oil to obtain an oil phase; and dropwise adding the oil phase into a water phase obtained by dissolving Tween-60 and glycerol in ultrapure water, stirring, shearing at a high speed, and homogenizing at a high pressure, thereby obtaining the product. The external skin-generating oil preparation disclosed by the invention has the effects of quickly reducing the depth and quantity of wrinkles in a short time.

Description

Technical Field

[0001] The present invention relates to the field of pharmaceutical technology, and particularly relates to a wrinkle-removing and anti-aging external skin-nourishing oil preparation that does not involve surgery or filling, and its preparation method and application. Background Art

[0002] Facial wrinkles are caused by degenerative changes in the skin structure (including reduced function of skin elastic fibers, degradation of dermal matrix, and relaxation of epidermal structure) due to various endogenous and / or exogenous factors stimulating the facial skin tissue, thereby forming visible folds or grooves. From a pathophysiological perspective, the formation of wrinkles is closely related to the reduction, denaturation, and disordered arrangement of collagen fibers (mainly type I and type III collagen) in the dermis, accompanied by the breakage and degradation of elastic fibers. In addition, ultraviolet radiation, oxidative stress, accumulation of advanced glycation end products, fluctuations in endocrine hormone levels (such as reduced estrogen), and environmental factors (such as pollution and dryness) can all accelerate the destruction of the extracellular matrix in skin tissue, inhibit the collagen synthesis ability of fibroblasts, resulting in a decrease in the mechanical support of the skin, and ultimately manifested as the formation and deepening of wrinkles. This process is one of the typical biological markers of skin aging.

[0003] The formation of wrinkles shows significant chronological characteristics and pathophysiological evolution rules. Generally, dynamic wrinkles related to the contraction of the orbicularis oculi muscle appear in the outer canthus (lateral orbital region) since the age of 18, and its formation mechanism involves the accumulation of microdamage to the dermal matrix caused by repeated facial muscle movements; after the age of 35, with the decline of dermal fibroblast activity, degradation of elastic fibers, and prolongation of the epidermal renewal cycle, persistent wrinkles with anatomical specificity are formed: including periorbital radial wrinkles (crow's feet), frontal muscle contraction transverse striations (forehead wrinkles), and annular folds related to the activity of the platysma muscle (neck wrinkles).

[0004] Histological studies have shown that the evolution of wrinkles goes through three stages: initial dynamic wrinkles (imbalance in the ratio of type I collagen / type III collagen), intermediate resting superficial wrinkles (rupture of the elastic fiber network), and late deep static wrinkles caused by flattening of the dermal-epidermal junction. This process is regulated by both intrinsic aging (telomere shortening, increased MMP enzyme activity) and extrinsic factors (photoaging-induced cross-linked collagen deposition). In other words, as the above-mentioned structural lesions evolve, wrinkles gradually change from shallow to deep, from dynamic to static, and finally to full-face wrinkles.

[0005] At present, wrinkle removal mainly includes two major categories: surgical (including facelift / or rhytidectomy, fractional laser therapy, radiofrequency therapy, etc.) and non-surgical methods. Surgical methods need to be implemented in a qualified and professional medical beauty center or the plastic surgery department of a high-level clinical hospital. However, the results often make the skin lines of the subjects appear misaligned, and the facial expressions look unnatural (in other words, the beauty achieved through surgery is not natural beauty). Non-surgical methods generally include (1) neuromuscular regulation (botulinum toxin type A inhibits the release of acetylcholine vesicles), (2) volume filling (construction of a three-dimensional network structure of sodium hyaluronate), (3) regenerative medicine (platelet-rich plasma releases PDGF / TGF-β), and (4) topical application of vitamin E (which can prevent the formation of skin brown pigments and has anti-aging properties; however, when using it, attention needs to be paid to the risk of allergy, and its treatment cycle is relatively long) and other topical preparations. Based on evidence-based medicine, a combined treatment plan can be considered. However, it should be fully understood that complications such as injection-induced myospasm, local induration, peripheral nerve injury, local infection, local aseptic inflammatory reaction, bleeding, and necrotizing fasciitis may occur through injection methods. Obviously, in addition to being extremely costly (basically a waste of money), there is also a possibility of causing local dysfunction, so that the parties pay a tragic and irreparable price of "disfiguring" (more of a loss than a gain).

[0006] Therefore, in order to prevent countless subjects, including women and men, from enduring endless pain (such as "silicone face") for the sake of short-term satisfaction, we need to find a method that neither relies on surgery nor requires filling, but instead uses a safe topical application method, and can significantly reduce the depth and number of facial wrinkles within a short period, gradually improve the skin condition (including water content, smoothness, texture, and elasticity, etc.), and significantly reduce the skin age, presenting a more youthful and healthy state. Summary of the Invention

[0007] Regarding the problem in the above-mentioned existing technologies of the lack of non-surgical and non-filling topical effective drugs for wrinkle removal and anti-aging:

[0008] The first object of the present invention is to provide a non-surgical and non-filling topical skin-nourishing oil preparation that can remove wrinkles and resist aging in a short period. The topical skin-nourishing oil preparation is obtained by substantially adjusting the early topical skin-nourishing oil formula (CN202410557338.0) of the inventor's team in order to solve the problems of wrinkles and old skin age.

[0009] The second object of the present invention is to provide a preparation method of the above-mentioned topical skin-nourishing oil preparation for wrinkle removal and anti-aging.

[0010] The third object of the present invention is to provide the medical use of the above-mentioned topical skin-nourishing oil preparation for wrinkle removal and anti-aging.

[0011] The inventive concept of the present invention is as follows:

[0012] Regarding the TCM diagnosis and treatment of facial wrinkles (facial manifestations of human skin aging), many doctors hold different views and have not yet reached a consensus. Based on the characteristic manifestations of facial wrinkles, the inventors have combined the strengths of many doctors and believe that it can be caused by the decline of Yangming pulse, the decline of the three Yang pulses, and the exhaustion of Yang Qi. Yangming has more blood and more Qi, and when it is exhausted, the blood and Qi cannot fill the pores, so the face wrinkles and muscles are exhausted; the three Yang pulses all go up to the head, so the exhaustion of the three Yang leads to deficiency of essence and blood, and blood is the mother of Qi. If the blood is deficient for a long time, Qi and blood will be deficient, and they cannot moisten the pores on the head and face, so wrinkles will appear. Considering the prevention and treatment of Yangming pulse deficiency, it is advisable to use spleen-strengthening and Qi-invigorating drugs while supplemented with products that lift and relieve muscles. The treatment is to invigorate Qi and build the middle, relieve muscles and smooth wrinkles. Medicinal materials such as ginseng, astragalus, licorice, white peony, cimicifuga, kudzu root, angelica, etc.; for the prevention and treatment of Sanyang pulse deficiency and Yang Qi exhaustion, the treatment is to replenish Yin and Yang, Qi and blood, nourish muscles and skin and remove wrinkles. Medicinal materials such as Rehmannia root, yam, cornus officinalis, Poria, angelica, wolfberry, Eucommia bark, Cuscuta australis, etc.

[0013] Combined with the fact that "Huangdi Neijing" points out that "meridians are the reason why we can decide life and death, treat all kinds of diseases, and adjust deficiency and excess, they must be unobstructed", the inventors considered the treatment principle of "invigorating qi and activating blood circulation, removing blood stasis and unblocking meridians", based on dredging local qi and blood meridians, improving the microcirculation of facial skin, improving the hypoxia of skin tissue, supplementing the nutrients needed by its cells, promoting cell metabolic activity, and restoring the mechanical properties of skin tissue, thereby effectively removing wrinkles and other skin aging manifestations.

[0014] In order to achieve the first purpose, the present invention adopts the following technical measures:

[0015] A skin-regenerating oil preparation for external use for wrinkle removal and anti-aging, the skin-regenerating oil preparation is a water-oil mixed preparation of skin-regenerating oil alcohol extract, and the solute of the skin-regenerating oil preparation is a freeze-dried powder of skin-regenerating oil alcohol extract; wherein the content of skin-regenerating oil alcohol extract in the water-oil mixed preparation of skin-regenerating oil alcohol extract is not less than 0.5mg / ml and not more than 5mg / ml, and preferably the content is not less than 1mg / ml and not more than 3mg / ml. The skin-regenerating oil alcohol extract is prepared by alcohol extraction of 9 raw materials in the following weight proportions (preferably, prepared by soaking in an ethanol solution with a volume fraction of 40%-90% for 2-3h): 20-50 weight parts of flat chrysanthemum radix notoginseng, 20-50 weight parts of dried tangerine peel, 5-15 weight parts of astragalus, 5-15 weight parts of angelica, 5-15 weight parts of frankincense, 5-15 weight parts of epimedium, 5-15 weight parts of scutellaria, 5-15 weight parts of phellodendron, and 3-8 weight parts of borneol.

[0016] Further, the preparation method of the freeze-dried powder of the ethanol extract of Shengyu Oil is as follows: Take the dry powders of the 8 raw medicinal materials of Shengyu Oil and borneol together in a container according to a certain weight part ratio, then add an ethanol solution with a volume fraction of 40%-90% to the container, soak for 2-3 hours, perform flash extraction 3-5 times, 5 minutes each time, concentrate the filtrate, and freeze-dry to obtain the freeze-dried powder of the ethanol extract of Shengyu Oil.

[0017] To achieve the second object, the present invention adopts the following technical measures:

[0018] A preparation method of the above-mentioned anti-wrinkle and anti-aging external Shengyu Oil preparation, the steps are as follows:

[0019] (1) Prepare the freeze-dried powder of the ethanol extract of Shengyu Oil: Take 500 g of 9 raw materials of Shengyu Oil according to a certain weight part ratio (the weight parts of each raw material are: Gynura procumbens 20-50 weight parts, tangerine peel 20-50 weight parts, astragalus membranaceus 5-15 weight parts, angelica sinensis 5-15 weight parts, frankincense 5-15 weight parts, epimedium 5-15 weight parts, scutellaria baicalensis 5-15 weight parts, phellodendron amurense 5-15 weight parts, borneol 3-8 weight parts). Except for borneol, the rest of the drugs are ground fine (passed through a 200-mesh sieve) and placed in a 10 L stainless steel barrel, add 3 L-7 L of ethanol with a volume fraction of 40%-90%, soak for 2-3 hours, perform flash extraction 3-5 times, 5 minutes each time, and quickly concentrate the filtered supernatant through a multi-stage flash evaporator, and then obtain the freeze-dried powder of the ethanol extract of Shengyu Oil through freeze-drying (the yield is 10.6%-12.9%).

[0020] (2) Prepare the Shengyu Oil preparation: Dissolve the freeze-dried powder of the ethanol extract of Shengyu Oil and Span 60 in edible vegetable oil to prepare an oil phase, and according to the volume ratio of the oil phase to the water phase of 1:10-30, drop the oil phase into the water phase prepared by dissolving Tween 60 and glycerol in ultrapure water, stir and then perform high-speed shearing and high-pressure homogenization to obtain; the zeta potential and particle size distribution of the obtained water-oil mixed preparation of the ethanol extract of Shengyu Oil are uniform; its particle size distribution is 58-1990 nm, and there is no multiple scattering phenomenon, and the average particle size is 438±21 nm; the average zeta potential is -50 mV to -40 mV. Among them, the edible vegetable oil is selected from at least one of olive oil, coconut oil and camellia oil. Preferably, the steps for preparing the Shengyu Oil preparation in step (2) are:

[0021] 1) Freeze-dry the ethanol extract powder of Shengfu oil and dissolve Span 60 in edible vegetable oil to prepare an oil phase; the final concentration of Span 60 is 0.5%-5% (W / V; g / ml); 2) Dissolve Tween 60 and glycerol in ultrapure water to prepare an aqueous phase; the final concentration of Tween 60 is 1%-3% (V / V); the final concentration of glycerol is 1%-10% (V / V); 3) Under the condition of a constant temperature water bath at 60°C, drop the oil phase prepared in step 1) into the aqueous phase prepared in step 2), and stir for 3 min under the condition of 3000 r / min; the volume ratio of the oil phase to the aqueous phase is 1:10-30; 4) High-speed shear for 2 min under the condition of 10000 r / min, and then high-pressure homogenize 6 times under the condition of 1000 bar to obtain the water-oil mixed preparation of the ethanol extract of Shengfu oil.

[0022] To achieve the third object of the present invention, the present invention also adopts the following technical measures:

[0023] The application of the above-mentioned external Shengfu oil preparation or the external Shengfu oil preparation prepared by the above method in the preparation of anti-wrinkle and anti-aging external drugs, especially in the preparation of non-surgical and non-filling anti-wrinkle and anti-aging external drugs. The anti-wrinkle and anti-aging type is for those with older age and / or poor skin quality.

[0024] The formulation principle of each traditional Chinese medicine raw material of the present invention is as follows:

[0025] Gynura procumbens, tangerine peel, astragalus, etc. tonify qi and promote qi movement, serving as the monarch drugs; angelica, frankincense, epimedium, etc. nourish blood and replenish essence, serving as the minister drugs; scutellaria baicalensis, phellodendron amurense, etc. cool the blood and dispel pathogenic factors to prevent stasis, serving as the assistant drugs; borneol and vegetable oil (using its viscosity to cover the skin, maintaining moisture in the form of an oil film and preventing dryness), serving as the envoy drugs. All the drugs work together to improve the microcirculation of facial skin, enable nutrients and oxygen to reach skin tissue cells, promote cell metabolic activity, restore the mechanical properties of skin tissue, thereby effectively reducing the depth and number of facial wrinkles, improving the skin state (including water content, smoothness, texture, elasticity, etc.), reducing the skin age, and finally achieving anti-wrinkle and anti-aging in a non-surgical and non-filling manner.

[0026] Compared with the prior art, the present invention has the following advantages and effects:

[0027] The research results show that after the skin-friendly oil preparation of the present invention is administered transdermally, it has the following advantages and effects: (1) It can be simply applied externally, with extremely simple operation and no skin damage (this point is incomparable to surgical filling), without worrying about ulceration within the skin like exogenous substances or autologous filling, which may harm the facial image; (2) The drug cost is low, without irritation, no toxic side effects, and there is no health threat (including deformation, infection, etc.) to the face caused by filling the skin tissue; (3) For people with older age and / or poorer skin quality, the repair effect is good. In the short term (within 15 days), the depth and number of facial wrinkles of the subjects are significantly reduced, the skin condition (including water content, smoothness, texture, and elasticity, etc.) is gradually improved, and their skin age is significantly reduced from large to small, presenting a more youthful state; (4) Comparing the touch before and after anti-wrinkle and anti-aging, in addition to the reduction in the depth and number of wrinkles, the feeling of silkiness and elasticity is enhanced after touching with the hand; (5) In terms of the use effect, it can completely replace the daily cumbersome moisturizing lotions, creams, and facial masks, and achieve a truly three-in-one anti-wrinkle, anti-aging, and skin-friendly oil preparation. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 It is a characterization diagram of the particle size and potential of the skin-friendly oil preparation prepared in Examples 1 and 2.

[0029] Figure 2 It is a diagram showing the influence of the skin-friendly oil preparation prepared in Example 1 on facial wrinkles and skin quality.

[0030] Figure 3 It is a diagram showing the influence of the skin-friendly oil preparation prepared in Example 1 on facial wrinkles and skin quality.

[0031] Figure 4 It is a diagram showing the influence of the skin-friendly oil preparation prepared in Example 1 on facial wrinkles and skin quality.

[0032] Figure 5 It is a diagram showing the influence of the skin-friendly oil preparation prepared in Example 1 on facial wrinkles and skin quality.

[0033] Figure 6 It is a diagram showing the influence of the skin-friendly oil preparation prepared in Example 2 on facial wrinkles and skin quality.

[0034] Figure 7 It is a diagram showing the influence of the skin-friendly oil preparation prepared in Example 2 on facial wrinkles and skin quality. DETAILED DESCRIPTION OF THE INVENTION

[0035] Hereinafter, the applicant will further elaborate on the technical solutions and applications of the present invention in combination with specific examples. It should be understood that the following content should not limit the protection scope of the present invention in any way.

[0036] In the embodiments of the present invention, the dry powders of 8 Chinese herbal medicine materials such as Gynura procumbens (Lour.) Merr., Pericarpium Citri Reticulatae, Astragalus membranaceus (Fisch.) Bge., Angelica sinensis (Oliv.) Diels, Epimedium brevicornu Maxim., Olibanum, Scutellaria baicalensis Georgi, and Phellodendron amurense Rupr. are all passed through a 200-mesh sieve.

[0037] Example 1: Preparation and Characterization of Skin-Healing Oil Preparation

[0038] 1) Preparation of Skin-Healing Oil Preparation

[0039] Preparation of freeze-dried powder of alcohol extract of skin-healing oil: Take 356 g of raw materials of skin-healing oil (100 g each of Gynura procumbens (Lour.) Merr. and Pericarpium Citri Reticulatae, 30 g each of dry powders of Astragalus membranaceus (Fisch.) Bge., Angelica sinensis (Oliv.) Diels, Olibanum, and Epimedium brevicornu Maxim., 10 g each of dry powders of Scutellaria baicalensis Georgi and Phellodendron amurense Rupr., and 16 g of borneol), stir evenly, place in a 5 L stainless steel bucket, add 4 L of 90 v / v% ethanol, soak for 2 h, perform flash extraction 3 times, 5 min each time, combine the supernatant liquids filtered three times, quickly concentrate through a multi-stage flash evaporator, and then obtain the freeze-dried powder of alcohol extract of skin-healing oil (37.8 g) through freeze-drying.

[0040] Formulation of skin-healing oil preparation: ① Prepare the oil phase by dissolving the freeze-dried powder of alcohol extract of skin-healing oil and Span 60 in olive oil, and the final concentration of Span 60 is 5% (W / V). ② Prepare the water phase by dissolving Tween 60 and glycerol in ultrapure water, the final concentration of Tween 60 is 3% (V / V), and the final concentration of glycerol is 1% (V / V). ③ Under the condition of a constant temperature water bath at 60 °C, drop the oil phase into the water phase, stir at 3000 r / min for 3 min, and the volume ratio of the oil phase to the water phase is 1:10. ④ Perform high-speed shearing at 10000 r / min for 2 min, and then perform high-pressure homogenization 6 times under the condition of 1000 bar to obtain the water-oil mixed preparation of alcohol extract of skin-healing oil, simply recorded as skin-healing oil preparation, and the content of alcohol extract of skin-healing oil in the obtained skin-healing oil preparation is equal to 3 mg / ml.

[0041] 2) Characterization of Particle Size and Zeta Potential of Skin-Healing Oil Preparation

[0042] When measuring the particle size of the skin-healing oil preparation, dilute the sample with ultrapure water so that its count rate is between 200 and 300 Kcps (1,000 counts per second), and then measure the particle size and polydispersity index with a laser particle size analyzer, scattering angle 90 °, temperature 25 °C; when measuring the zeta potential, adjust the conductivity of the skin-healing oil preparation to 50 S / cm with 0.9% (w / w) sodium chloride solution. As Figure 1 shown in (A, B) below, the particle size distribution of the skin-healing oil preparation prepared in Example 1 is in the range of 141 - 1290 nm, its average particle size is about 396 nm, and there is no multiple scattering phenomenon; moreover, its zeta potential is evenly distributed, with an average of -47.8 mV.

[0043] Example 2: Preparation and Characterization of Skin-Healing Oil Preparation

[0044] 1) Preparation of Skin-Generating Oil Preparation

[0045] Preparation of freeze-dried powder of ethanol extract of Skin-Generating Oil: Take 515 g of raw materials of Skin-Generating Oil (100 g each of Gynura procumbens and dried tangerine peel, 75 g each of dry powders of astragalus membranaceus, angelica sinensis, and frankincense, 25 g each of dry powders of epimedium brevicornum, scutellaria baicalensis, and phellodendron amurense, 15 g of borneol), stir evenly and place in a 5 L stainless steel bucket, add 4 L of 40 v / v% ethanol, soak for 3 h, perform flash extraction 5 times, 5 min each time, combine the supernatant liquids filtered five times and quickly concentrate through a multi-stage flash evaporator, and then obtain the freeze-dried powder of ethanol extract of Skin-Generating Oil (66.4 g) through freeze-drying.

[0046] Formulation of Skin-Generating Oil Preparation: ① Prepare the oil phase by dissolving the freeze-dried powder of ethanol extract of Skin-Generating Oil and span 60 in olive oil, and the final concentration of span 60 is 0.5% (W / V). ② Prepare the aqueous phase by dissolving tween 60 and glycerol in ultrapure water, the final concentration of tween 60 is 1% (V / V), and the final concentration of glycerol is 10% (V / V). ③ Under the condition of a constant temperature water bath at 60 °C, drop the oil phase into the aqueous phase, stir at 3000 r / min for 3 min, and the volume ratio of the oil phase to the aqueous phase is 1:30. ④ Perform high-speed shearing at 10000 r / min for 2 min, and then perform high-pressure homogenization 6 times at 1000 bar to obtain the water-oil mixed preparation of ethanol extract of Skin-Generating Oil, briefly recorded as Skin-Generating Oil Preparation. The content of the ethanol extract of Skin-Generating Oil in the obtained Skin-Generating Oil Preparation is equal to 1 mg / ml.

[0047] 2) Characterization of Particle Size and Zeta Potential of Skin-Generating Oil Preparation

[0048] When measuring the particle size of the Skin-Generating Oil Preparation, dilute the sample with ultrapure water so that its count rate is between 200 - 300 Kcps (1,000 counts per second), and then measure the particle size and polydispersity index with a laser particle size analyzer, scattering angle 90°, temperature 25 °C; when measuring the zeta potential, adjust the conductivity of the Skin-Generating Oil solution to 50 S / cm with 0.9% (w / w) sodium chloride solution. As Figure 1 shown in (C, D) below, the particle size distribution of the Skin-Generating Oil Preparation prepared in Example 2 is in the range of 58 - 1990 nm, the average particle size is about 458.7 nm, and there is no multiple scattering phenomenon; moreover, its zeta potential is evenly distributed, with an average of -44.2 mV.

[0049] Effect of the Skin-Generating Oil Preparation Prepared in Example 1 on Facial Wrinkles and Skin Quality in Example 3

[0050] A 72-year-old rural female subject has particularly dry skin due to years of working in the fields. She often feels tight and uncomfortable, especially when the climate changes or after a day of hard work. Her skin is relatively loose and dull, with wrinkles of different thicknesses and depths on her face, especially in areas with frequent facial expressions such as around the eyes and mouth. When touched, it feels obviously dry and rough.

[0051] Before evaluation: Wash the facial skin with warm water, then dry it with a towel. Then, drip 2 - 3 pumps (about 0.5 - 1 mL) of the skin-nourishing oil preparation prepared in Example 1 into the palm of the hand, and quickly and evenly spread it on the facial skin; once in the morning and once in the evening every day. To visually observe the usage effect of the skin care product, before using the product, as well as on the 5th, 10th, and 15th days of use, use a mobile phone (Apple iPhone 13, without beauty function) to take high-definition photos and the "PERFECT" intelligent AI skin detection system (https: / / www.perfectcorp.com / zh-cn / business / showcase / skincare / hd-diagnostics) to detect the skin condition for comparative analysis of the changes in facial skin.

[0052] Evaluation results: After using the product for a period of time, the subject felt that the discomfort such as dryness and roughness on the face improved, and it felt smoother and smoother when touched. The AI detection system showed that after using it for a period of time (5 days, 10 days, 15 days): (1) The skin health index gradually increased, and its skin age also changed from "68 years old", through "67 years old", "65 years old", to "64 years old" ( Figure 2 the upper two paragraphs of data); (2) Comparing Figure 2 the following two paragraphs of figures (the figure in the 4th paragraph is an enlarged view of the left corner of the eye area in the 3rd paragraph), it can be seen that for the skin wrinkles, whether it is the thickness, length, depth of the lines, or the number of wrinkles, they have significantly become narrower, shorter, shallower and fewer; (3) The facial skin texture of the subject has significantly become more delicate, the pores have shrunk, and the skin feels smoother.

[0053] Effect of the skin-nourishing oil preparation prepared in Example 1 on facial wrinkles and skin quality

[0054] A 69-year-old female patient has engaged in various occupations such as waste collection, nursing home caregiver, and farming, and has been exposed to wind and sun all year round. Her face is dry, rough, has a sense of tightness, and has many wrinkles (mainly concentrated in the areas around the two corners of the eyes and the forehead; of different thicknesses and depths, showing obvious unevenness).

[0055] Before evaluation: Moisten the facial skin with warm water, then blot it dry with a towel. Next, drip 2 - 3 pumps (about 0.5 - 1 mL) of the skin - nourishing oil preparation prepared in Example 1 onto the palm of your hand, and quickly and evenly spread it on the facial skin; do this once in the morning and once in the evening every day. To visually observe the usage effect of the skin care product, before using the product, as well as on the 5th, 10th, and 15th days of use, use a mobile phone (Apple iPhone 13, without beauty function) to take high - definition photos and the "PERFECT" intelligent AI skin detection system (https: / / www.perfectcorp.com / zh - cn / business / showcase / skincare / hd - diagnostics) to detect the skin condition for comparative analysis of facial skin changes.

[0056] Evaluation results: After using the product for a period of time, the subjects felt that the discomfort such as dryness and roughness on the face improved, and it felt smoother and smoother to the touch. The AI detection system showed that after using it for a period of time (5 days, 10 days, 15 days): (1) The skin health index gradually increased, and its skin age also changed from "76 years old", through "68 years old", "67 years old", to "66 years old" ( Figure 3 for the upper two paragraphs of data); (2) Comparing Figure 3 the following two paragraphs of figures (the figure in the 4th paragraph is an enlarged view of the area around the right - hand corner of the eye in the 3rd paragraph), it can be seen that for skin wrinkles, whether in terms of the thickness, length, depth of the wrinkles, or the number of wrinkles, they have significantly become narrower, shorter, shallower, and fewer; (3) The facial skin texture of the subjects has significantly become more delicate, the pores have shrunk, and its touch is smoother.

[0057] Effect of the skin - nourishing oil preparation prepared in Example 1 on facial wrinkles and skin quality in Example 5

[0058] A 59 - year - old female subject often had dry and hard facial skin, and facial wrinkles (mainly concentrated in the areas of both cheeks and the corners of the mouth) were very obvious. Usually, skin care products such as Dabao and Yuemeijing were applied to the face, but the self - perceived effect was average.

[0059] Before evaluation: Moisten the facial skin with warm water, then blot it dry with a towel. Next, drip 2 to 3 pumps (about 0.5 - 1 mL) of the skin-nourishing oil preparation prepared in Example 1 onto the palm of your hand, and quickly spread it evenly over the facial skin; do this once in the morning and once in the evening every day. To visually observe the effects of using the skin care product, before using the product and on the 5th, 10th, and 15th days of use, use a mobile phone (Apple iPhone 13 without beauty function) to take high-definition photos and the "PERFECT" intelligent AI skin detection system (https: / / www.perfectcorp.com / zh-cn / business / showcase / skincare / hd-diagnostics) to detect the skin condition for comparative analysis of facial skin changes.

[0060] Evaluation results: After using the product for a period of time, the subjects felt that the discomfort such as dryness and roughness of the face improved, and it felt smoother and smoother. The AI detection system showed that after using it for a period of time (5 days, 10 days, 15 days): (1) The skin health index gradually increased, and its skin age also changed from "65 years old", through "64 years old" and "62 years old", to "55 years old" ( Figure 4 for the upper two paragraphs of data); (2) Comparing Figure 4 the following two figures (the figure in the 4th paragraph is an enlarged view of the area near the corner of the mouth on the right cheek in the 3rd paragraph), it can be seen that for skin wrinkles, whether in terms of the thickness, length, depth of the wrinkles, or the number of wrinkles, they have significantly become narrower, shorter, shallower, and fewer; (3) The facial skin texture of the subjects has significantly become more delicate, and its touch is more mellow.

[0061] Effect of the skin-nourishing oil preparation prepared in Example 1 on facial wrinkles and skin quality in Example 6

[0062] A 70-year-old rural female subject rarely uses skin care products. Her facial skin shows a dry state, lacking the due moisture and luster, with a rough touch, and a dull complexion (her complexion shows an uneven state, accompanied by dullness, freckles or pigmentation).

[0063] Before evaluation: Moisten the facial skin with warm water, then blot it dry with a towel. Next, drip 2 to 3 pumps (about 0.5 - 1 mL) of the skin-nourishing oil preparation prepared in Example 1 onto the palm of your hand, and quickly spread it evenly over the facial skin; do this once in the morning and once in the evening every day. To visually observe the effects of the skin care product, before using the product and on the 5th, 10th, and 15th days of use, use a mobile phone (Apple iPhone 13, without beauty function) to take high-definition photos and the "PERFECT" intelligent AI skin detection system (https: / / www.perfectcorp.com / zh-cn / business / showcase / skincare / hd-diagnostics) to detect the skin condition for comparative analysis of facial skin changes.

[0064] Evaluation results: After using the product for a period of time, the subjects felt that the discomfort such as dryness and roughness on the face improved, and it felt smoother and smoother to the touch. The AI detection system showed that after using it for a period of time (5 days, 10 days, 15 days): (1) The skin health index gradually increased, and its skin age also changed from "68 years old", "67 years old", "66 years old" to "66 years old" ( Figure 5 for the upper two paragraphs of data); (2) Comparing Figure 5 the following two paragraphs of pictures (the picture in the 4th paragraph is an enlarged picture of the area around the right eye corner in the 3rd paragraph), it can be seen that for the facial skin wrinkles, both in terms of the thickness, length, depth of the wrinkles, and the number of wrinkles, they have significantly become narrower, shorter, shallower and fewer; (3) The facial skin texture of the subjects has significantly become more delicate, and its gloss has also gradually improved.

[0065] Effect of the skin-nourishing oil preparation prepared in Example 2 of Example 7 on facial wrinkles and skin quality

[0066] A 33-year-old female subject devotes most of her energy to her children and neglects her own skin care. Her face is dry, rough, and the skin is dull; there are short fine lines on the face and at the outer corners of the eyes; she has used many anti-aging products on the market before, but the effects were not good.

[0067] Before evaluation: Moisten the facial skin with warm water, then blot it dry with a towel. Next, drip 2 or 3 pumps (about 0.5 - 1 mL) of the skin-nourishing oil preparation prepared in Example 2 onto the palm of the hand, and quickly and evenly spread it on the facial skin. Do this once in the morning and once in the evening every day, and stop using other skin care products during this period. To visually observe the effects of the skin care product, before using the product and on the 9th, 13th, and 15th days of use, use a mobile phone (Apple iPhone 11 without beauty function) to take high-definition photos and the "PERFECT" intelligent AI skin detection system (https: / / www.perfectcorp.com / zh-cn / business / showcase / skincare / hd-diagnostics) to detect the skin condition for comparative analysis of facial skin changes.

[0068] Evaluation results: After using the product for a period of time, the subjects felt that the discomfort such as dryness and roughness on the face improved, and it felt smoother and smoother to the touch. The AI detection system showed that after using it for a period of time (9 days, 13 days, 15 days): (1) The skin health index gradually increased, and the skin age also changed from "27 years old", through "24 years old", "22 years old", to "21 years old" ( Figure 6 ); (2) The facial skin texture of the subjects became significantly finer, the pores shrank, and the touch became smoother. (3) The facial dullness of the subjects gradually improved, and they looked fairer and brighter.

[0069] Effect of the skin-nourishing oil preparation prepared in Example 2 on facial wrinkles and skin quality in Example 8

[0070] A 39-year-old female subject has been working on a construction site all year round, exposed to wind and sun. She feels that her face is dry, her pores are a bit large, her skin color is dull, and wrinkles are visible (mainly concentrated in the areas of the two outer corners of the eyes and the forehead; short fine lines), etc. She usually uses basic hydrating skin care products, but the effect is minimal.

[0071] Before evaluation: Moisten the facial skin with warm water, then blot it dry with a towel. Next, drip 2 or 3 pumps (about 0.5 - 1 mL) of the skin-nourishing oil preparation prepared in Example 2 onto the palm of the hand, and quickly and evenly spread it on the facial skin. Do this once in the morning and once in the evening every day. To visually observe the effects of the skin care product, before using the product and on the 9th, 13th, and 15th days of use, use a mobile phone (Honor 80 SE without using beauty function) to take high-definition photos and the "PERFECT" intelligent AI skin detection system (https: / / www.perfectcorp.com / zh-cn / business / showcase / skincare / hd-diagnostics) to detect the skin condition for comparative analysis of facial skin changes.

[0072] Evaluation results: After using the product for a period of time, the subjects felt that the discomfort such as dryness and roughness of the face improved. The AI detection system showed that after using it for a period of time (9 days, 13 days, 15 days): (1) The skin health index gradually increased, and its skin age also changed from "38 years old" to "30 years old" through "34 years old" and "32 years old"( Figure 7 ); (2) It can be seen that the eye wrinkles on the skin become flatter and fewer; (3) The facial skin texture of the subjects becomes finer, the pores shrink, and the skin color becomes brighter.

Claims

1. An external skin-nourishing oil preparation for removing wrinkles and anti-aging, characterized in that, The skin-healing oil preparation is an aqueous-oil mixed preparation of the ethanol extract of skin-healing oil, and the content of the ethanol extract of skin-healing oil in the aqueous-oil mixed preparation of the ethanol extract of skin-healing oil is not less than 0.5 mg / ml and not more than 5 mg / ml; The preparation method of the skin-healing oil preparation is as follows: Dissolve the freeze-dried powder of the ethanol extract of skin-healing oil and Span 60 in edible vegetable oil to prepare an oil phase, and drop the oil phase into the aqueous phase prepared by dissolving Tween 60 and glycerol in ultrapure water according to the volume ratio of the oil phase to the aqueous phase of 1:10 - 30, stir, and then perform high-speed shearing and high-pressure homogenization to obtain the aqueous-oil mixed preparation of the ethanol extract of skin-healing oil; The preparation method of the freeze-dried powder of the ethanol extract of skin-healing oil is as follows: Take the dry powders of 8 raw material herbs of skin-healing oil and borneol together in a certain weight ratio and add them to a container, then add an ethanol solution with a volume fraction of 40% - 90% to the container, soak for 2 - 3 h, then perform flash extraction, concentrate the filtrate, and freeze-dry to obtain the freeze-dried powder of the ethanol extract of skin-healing oil; The 8 raw material herbs of skin-healing oil are Gynura procumbens (Lour.) Merr., Citrus reticulata Blanco, Astragalus membranaceus (Fisch.) Bunge, Angelica sinensis (Oliv.) Diels, Boswellia carterii Birdw., Epimedium brevicornu Maxim., Scutellaria baicalensis Georgi, and Phellodendron amurense Rupr.; The weight ratios of each raw material herb and borneol are as follows: 20 - 50 parts by weight of Gynura procumbens (Lour.) Merr., 20 - 50 parts by weight of Citrus reticulata Blanco, 5 - 15 parts by weight of Astragalus membranaceus (Fisch.) Bunge, 5 - 15 parts by weight of Angelica sinensis (Oliv.) Diels, 5 - 15 parts by weight of Boswellia carterii Birdw., 5 - 15 parts by weight of Epimedium brevicornu Maxim., 5 - 15 parts by weight of Scutellaria baicalensis Georgi, 5 - 15 parts by weight of Phellodendron amurense Rupr., and 3 - 8 parts by weight of borneol.

2. The external skin healing oil preparation according to claim 1, wherein The content of the ethanol extract of skin-healing oil in the aqueous-oil mixed preparation of the ethanol extract of skin-healing oil is not less than 1 mg / ml and not more than 3 mg / ml.

3. The external skin-healing oil preparation according to claim 1, characterized in that, The edible vegetable oil is selected from at least one of olive oil, coconut oil, and camellia oil.

4. The external skin healing oil preparation according to claim 1, wherein The relationship between the total mass of the 9 raw materials of skin-healing oil and the dosage of the ethanol solution with a volume fraction of 40% - 90% is 500 g : (3 - 7) L.

5. The external skin-healing oil preparation according to claim 1, wherein The dry powders of the 8 herbs of Gynura procumbens (Lour.) Merr., Citrus reticulata Blanco, Astragalus membranaceus (Fisch.) Bunge, Angelica sinensis (Oliv.) Diels, Boswellia carterii Birdw., Epimedium brevicornu Maxim., Scutellaria baicalensis Georgi, and Phellodendron amurense Rupr. all pass through a 200-mesh sieve.

6. The preparation method of the external skin-healing oil preparation according to any one of claims 1 to 5, characterized in that, The preparation method of the skin-healing oil preparation is as follows: (1) Prepare the freeze-dried powder of the ethanol extract of skin-healing oil: Take the dry powders of 8 raw material herbs of skin-healing oil and borneol together in a certain weight ratio and add them to a container, then add an ethanol solution with a volume fraction of 40% - 90% to the container, soak for 2 - 3 h, perform flash extraction 3 - 5 times, 5 min each time, concentrate the filtrate, and freeze-dry to obtain the freeze-dried powder of the ethanol extract of skin-healing oil; (2) Prepare the skin-healing oil preparation: 1) Dissolve the freeze-dried powder of the ethanol extract of skin-healing oil and Span 60 in edible vegetable oil to prepare an oil phase; the final concentration of Span 60 is 0.5% - 5% W / V; 2) Dissolve Tween 60 and glycerol in ultrapure water to prepare an aqueous phase; the final concentration of Tween 60 is 1% - 3% V / V; the final concentration of glycerol is 1% - 10% V / V; 3) Under the condition of a constant temperature water bath at 60 °C, drop the oil phase prepared in step 1) into the aqueous phase prepared in step 2), and stir at 3000 r / min for 3 min; the volume ratio of the oil phase to the aqueous phase is 1:10 - 30; 4) High shear for 2 min under the condition of 10,000 r / min, and then high-pressure homogenize 6 times under the condition of 1000 bar to obtain the water-oil mixed preparation of the ethanol extract of raw skin oil.

7. The preparation method according to claim 6, wherein The zeta potential and particle size distribution of the obtained water-oil mixed preparation of the ethanol extract of raw skin oil are uniform. Its particle size distribution is 58 - 1990 nm, and there is no multiple scattering phenomenon. The average particle size is 438 ± 21 nm; the average zeta potential is -50 mV to -40 mV.

8. Use of the external raw skin oil preparation according to any one of claims 1 - 5 or the external raw skin oil preparation prepared by the method according to any one of claims 6 - 7 in the preparation of an external anti-wrinkle and anti-aging drug, especially in the preparation of an external anti-wrinkle and anti-aging drug without surgery and non-filling.

9. The application according to claim 8, wherein The anti-wrinkle and anti-aging type is for those with relatively older age and / or poor skin quality.

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